Publications

evidence for the induction of key components of the notch signaling pathway via deltamethrin and azamethiphos treatment in the sea louse caligus rogercresseyi.	the extensive use of organophosphates and pyrethroids in the aquaculture industry has negatively impacted parasite sensitivity to the delousing effects of these antiparasitics, especially among sea lice species. the notch signaling pathway is a positive regulator of abc transporter subfamily c expression and plays a key role in the generation and modulation of pesticide resistance. however, little is known about the molecular mechanisms behind pesticide resistance, partly due to the lack of geno ...	2016	27187362
wolbachia blocks viral genome replication early in infection without a transcriptional response by the endosymbiont or host small rna pathways.	the intracellular endosymbiotic bacterium wolbachia can protect insects against viral infection, and is being introduced into mosquito populations in the wild to block the transmission of arboviruses that infect humans and are a major public health concern. to investigate the mechanisms underlying this antiviral protection, we have developed a new model system combining wolbachia-infected drosophila melanogaster cell culture with the model mosquito-borne semliki forest virus (sfv; togaviridae, a ...	2016	27089431
zika virus strains potentially display different infectious profiles in human neural cells.	the recent zika virus (zikv) epidemic has highlighted the poor knowledge on its physiopathology. recent studies showed that zikv of the asian lineage, responsible for this international outbreak, causes neuropathology in vitro and in vivo. however, two african lineages exist and the virus is currently found circulating in africa. the original african strain was also suggested to be neurovirulent but its laboratory usage has been criticized due to its multiple passages. in this study, we compared ...	2016	27688094
pirna pathway is not required for antiviral defense in drosophila melanogaster.	since its discovery, rna interference has been identified as involved in many different cellular processes, and as a natural antiviral response in plants, nematodes, and insects. in insects, the small interfering rna (sirna) pathway is the major antiviral response. in recent years, the piwi-interacting rna (pirna) pathway also has been implicated in antiviral defense in mosquitoes infected with arboviruses. using drosophila melanogaster and an array of viruses that infect the fruit fly acutely o ...	2016	27357659
yellow fever virus capsid protein is a potent suppressor of rna silencing that binds double-stranded rna.	mosquito-borne flaviviruses, including yellow fever virus (yfv), zika virus (zikv), and west nile virus (wnv), profoundly affect human health. the successful transmission of these viruses to a human host depends on the pathogen's ability to overcome a potentially sterilizing immune response in the vector mosquito. similar to other invertebrate animals and plants, the mosquito's rna silencing pathway comprises its primary antiviral defense. although a diverse range of plant and insect viruses has ...	2016	27849599
genome investigations of vector competence in aedes aegypti to inform novel arbovirus disease control approaches.	dengue (denv), yellow fever, chikungunya, and zika virus transmission to humans by a mosquito host is confounded by both intrinsic and extrinsic variables. besides virulence factors of the individual arboviruses, likelihood of virus transmission is subject to variability in the genome of the primary mosquito vector, aedes aegypti. the "vectorial capacity" of a. aegypti varies depending upon its density, biting rate, and survival rate, as well as its intrinsic ability to acquire, host and transmi ...	2016	27809220
climate change influences on the global potential distribution of the mosquito culex quinquefasciatus, vector of west nile virus and lymphatic filariasis.	rapid emergence of most vector-borne diseases (vbds) may be associated with range expansion of vector populations. culex quinquefasciatus say 1823 is a potential vector of west nile virus, saint louis encephalitis virus, and lymphatic filariasis. we estimated the potential distribution of cx. quinquefasciatus under both current and future climate conditions. the present potential distribution of cx. quinquefasciatus showed high suitability across low-latitude parts of the world, reflecting the c ...	2016	27695107
morphological abnormalities and cell death in the asian citrus psyllid (diaphorina citri) midgut associated with candidatus liberibacter asiaticus.	candidatus liberibacter asiaticus (clas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known as huanglongbing (hlb). clas is transmitted by the asian citrus psyllid (acp) diaphorina citri, in a circulative manner. two major barriers to transmission within the insect are the midgut and the salivary glands. we performed a thorough microscopic analysis within the insect midgut following exposure to clas-infected cit ...	2016	27630042
the strica homolog aacasps16 is involved in apoptosis in the yellow fever vector, aedes albopictus.	caspases are a family of cysteine proteases playing essential roles during apoptosis. seven caspases identified in drosophila were dronc, dredd, strica, dcp-1, decay, drice and damm. among them, strica is an insect-specific caspase containing a long serine- and threonine- rich prodomain, of which function is not yet well studied. here we identified a homolog of strica from aedes albopictus, named as aacasps16. aacasps16 encoded a protein containing a putative serine- and threonine-rich prodomain ...	2016	27351972
mosquito saliva increases endothelial permeability in the skin, immune cell migration, and dengue pathogenesis during antibody-dependent enhancement.	dengue remains the most prevalent arthropod-borne viral disease in humans. while probing for blood vessels, aedes aegypti and ae. albopictus mosquitoes transmit the four serotypes of dengue virus (denv1-4) by injecting virus-containing saliva into the skin. even though arthropod saliva is known to facilitate transmission and modulate host responses to other pathogens, the full impact of mosquito saliva on dengue pathogenesis is still not well understood. inoculating mice lacking the interferon-α ...	2016	27310141
a glance at subgenomic flavivirus rnas and micrornas in flavivirus infections.	the family flaviviridae comprises a wide variety of viruses that are distributed worldwide, some of which are associated with high rates of morbidity and mortality. there are neither vaccines nor antivirals for most flavivirus infections, reinforcing the importance of research on different aspects of the viral life cycle. during infection, cytoplasmic accumulation of rna fragments mainly originating from the 3' utrs, which have been designated subgenomic flavivirus rnas (sfrnas), has been detect ...	2016	27233361
infection pattern and transmission potential of chikungunya virus in two new world laboratory-adapted aedes aegypti strains.	chikungunya virus (chikv) is an emerging mosquito-borne virus belonging to the togaviridae, which is transmitted to humans by aedes aegypti and ae. albopictus. we describe the infection pattern of chikv in two new world ae. aegypti strains, hwe and orl. both mosquito strains were susceptible to the virus but showed different infection patterns in midguts and salivary glands. even though acquisition of a bloodmeal showed moderate levels of apoptosis in midgut tissue, there was no obvious addition ...	2016	27102548
endogenously-expressed nh2-terminus of circumsporozoite protein interferes with sporozoite invasion of mosquito salivary glands.	the circumsporozoite protein is the most abundant polypeptide expressed by sporozoites, the malaria parasite stage capable of infecting humans. sporozoite invasion of mosquito salivary glands prior to transmission is likely mediated by a receptor/ligand-like interaction of the parasites with the target tissues, and the amino (nh2)-terminal portion of csp is involved in this interaction but not the tsr region on the carboxyl (c)-terminus. peptides based on the nh2-terminal domain could compete wi ...	2016	26964736
genomic approaches for understanding dengue: insights from the virus, vector, and host.	the incidence and geographic range of dengue have increased dramatically in recent decades. climate change, rapid urbanization and increased global travel have facilitated the spread of both efficient mosquito vectors and the four dengue virus serotypes between population centers. at the same time, significant advances in genomics approaches have provided insights into host-pathogen interactions, immunogenetics, and viral evolution in both humans and mosquitoes. here, we review these advances an ...	2016	26931545
small rna profiling in dengue virus 2-infected aedes mosquito cells reveals viral pirnas and novel host mirnas.	in aedes mosquitoes, infections with arthropod-borne viruses (arboviruses) trigger or modulate the expression of various classes of viral and host-derived small rnas, including small interfering rnas (sirnas), piwi interacting rnas (pirnas), and micrornas (mirnas). viral sirnas are at the core of the antiviral rna interference machinery, one of the key pathways that limit virus replication in invertebrates. besides sirnas, aedes mosquitoes and cells derived from these insects produce arbovirus-d ...	2016	26914027
transstadial effects of bti on traits of aedes aegypti and infection with dengue virus.	most mosquito control efforts are primarily focused on reducing the adult population size mediated by reductions in the larval population, which should lower risk of disease transmission. although the aim of larviciding is to reduce larval abundance and thus recruitment of adults, nonlethal effects on adults are possible, including transstadial effects on phenotypes of adults such as survival and pathogen infection and transmission. in addition, the mortality induced by control efforts may act i ...	2016	26871951
inhibition of chikungunya virus replication by 1-[(2-methylbenzimidazol-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea(mbzm-n-ibt).	chikungunya virus (chikv) infection is one of the most challenging human arboviral infections with global significance and without any specific antiviral. in this investigation, 1-[(2-methylbenzimidazol-1-yl) methyl]-2-oxo-indolin-3-ylidene] amino] thiourea (mbzm-n-ibt) was synthesised as a molecular hybrid of 2-methyl benzimidazole and isatin-β-thiosemicarbazone and its anti-chikv property was evaluated. the release of infectious virus particles was calculated by plaque assay, expression profil ...	2016	26843462
apoptosis, autophagy and unfolded protein response pathways in arbovirus replication and pathogenesis.	arboviruses are pathogens that widely affect the health of people in different communities around the world. recently, a few successful approaches toward production of effective vaccines against some of these pathogens have been developed, but treatment and prevention of the resulting diseases remain a major health and research concern. the arbovirus infection and replication processes are complex, and many factors are involved in their regulation. apoptosis, autophagy and the unfolded protein r ...	2016	26781343
viral metagenomics on blood-feeding arthropods as a tool for human disease surveillance.	surveillance and monitoring of viral pathogens circulating in humans and wildlife, together with the identification of emerging infectious diseases (eids), are critical for the prediction of future disease outbreaks and epidemics at an early stage. it is advisable to sample a broad range of vertebrates and invertebrates at different temporospatial levels on a regular basis to detect possible candidate viruses at their natural source. however, virus surveillance systems can be expensive, costly i ...	2016	27775568
an updated checklist of mosquito species (diptera: culicidae) from madagascar.	an updated checklist of 235 mosquito species from madagascar is presented. the number of species has increased considerably compared to previous checklists, particularly the last published in 2003 (178 species). this annotated checklist provides concise information on endemism, taxonomic position, developmental stages, larval habitats, distribution, behavior, and vector-borne diseases potentially transmitted. the 235 species belong to 14 genera: aedeomyia (3 species), aedes (35 species), anophel ...	2016	27101839
the insect-specific palm creek virus modulates west nile virus infection in and transmission by australian mosquitoes.	insect-specific viruses do not replicate in vertebrate cells, but persist in mosquito populations and are highly prevalent in nature. these viruses may naturally regulate the transmission of pathogenic vertebrate-infecting arboviruses in co-infected mosquitoes. following the isolation of the first australian insect-specific flavivirus (isf), palm creek virus (pcv), we investigated routes of infection and transmission of this virus in key australian arbovirus vectors and its impact on replication ...	2016	27457250
development and validation of a quantitative, one-step, multiplex, real-time reverse transcriptase pcr assay for detection of dengue and chikungunya viruses.	dengue virus (denv) and chikungunya virus (chikv) are important human pathogens with common transmission vectors and similar clinical presentations. patient care may be impacted by the misdiagnosis of denv and chikv in areas where both viruses cocirculate. in this study, we have developed and validated a one-step multiplex reverse transcriptase pcr (rt-pcr) to simultaneously detect, quantify, and differentiate between four denv serotypes (pan-denv) and chikungunya virus. the assay uses taqman te ...	2016	27098955
vector competence of aedes aegypti and aedes polynesiensis populations from french polynesia for chikungunya virus.	from october 2014 to march 2015, french polynesia experienced for the first time a chikungunya outbreak. two aedes mosquitoes may have contributed to chikungunya virus (chikv) transmission in french polynesia: the worldwide distributed ae. aegypti and the polynesian islands-endemic ae. polynesiensis mosquito.	2016	27144888
vertical transmission of indian ocean lineage of chikungunya virus in aedes aegypti and aedes albopictus mosquitoes.	the re-emergence of chikungunya (chik) fever in thailand has been caused by a novel lineage of chikungunya virus (chikv) termed the indian ocean lineage (iol). the aedes albopictus mosquito is thought to be a primary vector of chik fever in thailand, whereas ae. aegypti acts as a secondary vector of the virus. the vertical transmission is believed to be a primary means to maintain chikv in nature and may be associated with an increased risk of outbreak. therefore, the goal of this study was to a ...	2016	27108077
membrane feeding of dengue patient's blood as a substitute for direct skin feeding in studying aedes-dengue virus interaction.	understanding the interaction between aedes vectors and dengue viruses (denv) has significant implications in determining the transmission dynamics of dengue. the absence of an animal model and ethical concerns regarding direct feeding of mosquitoes on patients has resulted in most infection studies using blood meals spiked with laboratory-cultured denv. data obtained from such studies may not reflect the natural human-mosquito transmission scenario. this study explored the potential of using me ...	2016	27083158
rapid detection strategies for the global threat of zika virus: current state, new hypotheses, and limitations.	the current scenario regarding the widespread zika virus (zikv) has resulted in numerous diagnostic studies, specifically in south america and in locations where there is frequent entry of travelers returning from zikv-affected areas, including pregnant women with or without clinical symptoms of zikv infection. the world health organization, who, announced that millions of cases of zikv are likely to occur in the usa in the near future. this situation has created an alarming public health emerge ...	2016	27822207
characterization of a novel murine model to study zika virus.	the mosquito-borne zika virus (zikv) is responsible for an explosive ongoing outbreak of febrile illness across the americas. zikv was previously thought to cause only a mild, flu-like illness, but during the current outbreak, an association with guillain-barré syndrome and microcephaly in neonates has been detected. a previous study showed that zikv requires murine adaptation to generate reproducible murine disease. in our study, a low-passage cambodian isolate caused disease and mortality in m ...	2016	27022155
epidemiology of emergent madariaga encephalitis in a region with endemic venezuelan equine encephalitis: initial host studies and human cross-sectional study in darien, panama.	neurotropic arboviral infections are an important cause of encephalitis. a zoonotic, vector-borne alphavirus, madariaga virus (madv; formerly known as south american eastern equine encephalitis virus), caused its first documented human outbreak in 2010 in darien, panama, where the genetically similar venezuelan equine encephalitis virus (veev) is endemic. we report the results of a seroprevalence survey of animals and humans, illustrating contrasting features of madv and veev ecology and epidemi ...	2016	27101567
the role of the membrane in the structure and biophysical robustness of the dengue virion envelope.	the dengue virion is surrounded by an envelope of membrane proteins surrounding a lipid bilayer. we have combined the cryoelectron microscopy structures of the membrane proteins (pdb: 3j27) with a lipid bilayer whose composition is based on lipidomics data for insect cell membranes, to obtain a near-atomic resolution computational model of the envelope of the dengue virion. a coarse-grained molecular dynamics simulation on the microsecond timescale enables analysis of key biophysical properties ...	2016	26833387
intra-epidemic evolutionary dynamics of a dengue virus type 1 population reveal mutant spectra that correlate with disease transmission.	dengue virus (denv) is currently the most prevalent mosquito-borne viral pathogen. denvs naturally exist as highly heterogeneous populations. even though the descriptions on denv diversity are plentiful, only a few studies have narrated the dynamics of intra-epidemic virus diversity at a fine scale. such accounts are important to decipher the reciprocal relationship between viral evolutionary dynamics and disease transmission that shape dengue epidemiology. in the current study, we present a mic ...	2016	26940650
zika virus and its implication in transfusion safety.		2016	26969782
transcriptome analysis of the midgut of the chinese oak silkworm antheraea pernyi infected with antheraea pernyi nucleopolyhedrovirus.	the antheraea pernyi nucleopolyhedrovirus (apnpv) is an exclusive pathogen of a. pernyi. the intense interactions between apnpv and a. pernyi cause a series of physiological and pathological changes to a. pernyi. however, no detailed report exists regarding the molecular mechanisms underlying the interactions between apnpv and a. pernyi. in this study, four cdna libraries of the a. pernyi midgut, including two apnpv-infected groups and two control groups, were constructed for transcriptomic anal ...	2016	27820844
prolonged shedding of chikungunya virus in semen and urine: a new perspective for diagnosis and implications for transmission.	we report the presence of chikungunya (chikv) rna in the blood, urine and semen during the acute phase of the disease in an adult with a dual infection with dengue virus type 3. the patient, a 25 yr-old man from salvador, brazil, reported a 6-day duration of high fever, arthralgia, myalgia, headache and photophobia.blood and semen specimens were positive for chikv in the first collected samples; semen and urine specimens were positive for chikv after 30 days of symptoms onset. denv-3 rna was pos ...	2016	27882301
genome sequence of dengue virus 3 from the pythium insidiosum transcriptomes.		2016	27379056
zika virus: new clinical syndromes and its emergence in the western hemisphere.	zika virus (zikv) had remained a relatively obscure flavivirus until a recent series of outbreaks accompanied by unexpectedly severe clinical complications brought this virus into the spotlight as causing an infection of global public health concern. in this review, we discuss the history and epidemiology of zikv infection, recent outbreaks in oceania and the emergence of zikv in the western hemisphere, newly ascribed complications of zikv infection, including guillain-barré syndrome and microce ...	2016	26962217
the global spread of zika virus: is public and media concern justified in regions currently unaffected?	zika virus, an aedes mosquito-borne flavivirus, is fast becoming a worldwide public health concern following its suspected association with over 4000 recent cases of microcephaly among newborn infants in brazil.	2016	27093860
zika virus: the latest newcomer.	since the beginning of this century, humanity has been facing a new emerging, or re-emerging, virus threat almost every year: west nile, influenza a, avian flu, dengue, chikungunya, sars, mers, ebola, and now zika, the latest newcomer. zika virus (zikv), a flavivirus transmitted by aedes mosquitoes, was identified in 1947 in a sentinel monkey in uganda, and later on in humans in nigeria. the virus was mainly confined to the african continent until it was detected in south-east asia the 1980's, t ...	2016	27148186
clinical and epidemiological characterization of laboratory-confirmed autochthonous cases of zika virus disease in mexico.	since 2014, autochthonous circulation of zika virus (zikv) in the americas was detected (easter island, chile). in may 2015, brazil confirmed autochthonous --transmission and in october of that year colombia reported their first cases. now more than 52 countries have reported cases, including mexico. to deal with this contingency in mexico, several surveillance systems, in addition to systems for vector-borne diseases were strengthened with the participation of all health institutions. also, th ...	2016	27158557
computational approach towards exploring potential anti-chikungunya activity of selected flavonoids.	chikungunya virus (chikv) is a mosquito-borne alphavirus that causes chikungunya infection in humans. despite the widespread distribution of chikv, no antiviral medication or vaccine is available against this virus. therefore, it is crucial to find an effective compound to combat chikv. we aimed to predict the possible interactions between non-structural protein 3 (nsp) of chikv as one of the most important viral elements in chikv intracellular replication and 3 potential flavonoids using a comp ...	2016	27071308
zika virus infections imported from brazil to portugal, 2015.	zika virus is an emerging arbovirus transmitted by aedes sp. mosquitoes like the dengue and chikungunya viruses. zika virus was until recently considered a mild pathogenic mosquito-borne flavivirus with very few reported benign human infections. in 2007, an epidemic in micronesia initiated the turnover in the epidemiological history of zika virus and more recently, the potential association with congenital microcephaly cases in brazil 2015, still under investigation, led the world health organiz ...	2016	27134823
estimating risks of importation and local transmission of zika virus infection.	background. an international spread of zika virus (zikv) infection has attracted global attention. zikv is conveyed by a mosquito vector, aedes species, which also acts as the vector species of dengue and chikungunya viruses. methods. arrival time of zikv importation (i.e., the time at which the first imported case was diagnosed) in each imported country was collected from publicly available data sources. employing a survival analysis model in which the hazard is an inverse function of the effec ...	2016	27069825
identify-isolate-inform: a tool for initial detection and management of zika virus patients in the emergency department.	first isolated in 1947 from a monkey in the zika forest in uganda, and from mosquitoes in the same forest the following year, zika virus has gained international attention due to concerns for infection in pregnant women potentially causing fetal microcephaly. more than one million people have been infected since the appearance of the virus in brazil in 2015. approximately 80% of infected patients are asymptomatic. an association with microcephaly and other birth defects as well as guillain-barre ...	2016	27330653
recombinant dengue 2 virus ns3 helicase protein enhances antibody and t-cell response of purified inactivated vaccine.	dengue virus purified inactivated vaccines (piv) are highly immunogenic and protective over the short term, but may be poor at inducing cell-mediated immune responses and long-term protection. the dengue nonstructural protein 3 (ns3) is considered the main target for t-cell responses during viral infection. the amino (n)-terminal protease and the carboxy (c)-terminal helicase domains of denv-2 ns3 were expressed in e. coli and analyzed for their immune-potentiating capacity. mice were immunized ...	2016	27035715
zika virus: diagnostics for an emerging pandemic threat.	zika virus (zikv) is anaedesmosquito-borne flavivirus that emerged in brazil in 2015 and then rapidly spread throughout the tropical and subtropical americas. based on clinical criteria alone, zikv cannot be reliably distinguished from infections with other pathogens that cause an undifferentiated systemic febrile illness, including infections with two common arboviruses, dengue virus and chikungunya virus. this minireview details the methods that are available to diagnose zikv infection.	2016	26888897
maternal antibody-mediated disease enhancement in type i interferon-deficient mice leads to lethal disease associated with liver damage.	epidemiological studies have reported that most of the severe dengue cases occur upon a secondary heterologous infection. furthermore, babies born to dengue immune mothers are at greater risk of developing severe disease upon primary infection with a heterologous or homologous dengue virus (denv) serotype when maternal antibodies reach sub-neutralizing concentrations. these observations have been explained by the antibody mediated disease enhancement (ade) phenomenon whereby heterologous antibod ...	2016	27007501
nucleotide composition of the zika virus rna genome and its codon usage.	rna viruses have genomes with a distinct nucleotide composition and codon usage. we present the global characteristics of the rna genome of zika virus (zikv), an emerging pathogen within the flavivirus genus. zikv was first isolated in 1947 in uganda, caused a widespread epidemic in south and central america and the caribbean in 2015 and has recently been associated with microcephaly in newborns.	2016	27278486
pathogenesis of zika virus-associated embryopathy.	a strong causal association has become evident between zika virus (zikv) infection during pregnancy and the occurrence of fetal growth restriction, microcephaly and eye defects. circumstantial evidence is presented in this paper in support of the hypothesis that these effects, as well as the guillain-barré syndrome, are due to an endogenous form of hypervitaminosis a resulting from zikv infection-induced damage to the liver and the spillage of stored vitamin a compounds ("retinoids") into the ma ...	2016	27403405
long-term safety and immunogenicity of a tetravalent live-attenuated dengue vaccine and evaluation of a booster dose administered to healthy thai children.	we evaluated the safety and immunogenicity of two doses of a live-attenuated, tetravalent dengue virus vaccine (f17/pre formulation) and a booster dose in a dengue endemic setting in two studies. seven children (7- to 8-year-olds) were followed for 1 year after dose 2 and then given a booster dose (f17/pre formulation), and followed for four more years (child study). in the infant study, 49 2-year-olds, vaccinated as infants, were followed for approximately 3.5 years after dose 2 and then given ...	2016	27022153
scoping review of the zika virus literature.	the global primary literature on zika virus (zikv) (n = 233 studies and reports, up to march 1, 2016) has been compiled using a scoping review methodology to systematically identify and characterise the literature underpinning this broad topic using methods that are documented, updateable and reproducible. our results indicate that more than half the primary literature on zikv has been published since 2011. the articles mainly covered three topic categories: epidemiology of zikv (surveillance an ...	2016	27244249
quantifying the epidemiological impact of vector control on dengue.		2016	27227829
a pan-dengue virus reverse transcription-insulated isothermal pcr assay intended for point-of-need diagnosis of dengue virus infection by use of the pockit nucleic acid analyzer.	dengue virus (denv) infection is considered a major public health problem in developing tropical countries where the virus is endemic and continues to cause major disease outbreaks every year. here, we describe the development of a novel, inexpensive, and user-friendly diagnostic assay based on a reverse transcription-insulated isothermal pcr (rt-iipcr) method for the detection of all four serotypes of denv in clinical samples. the diagnostic performance of the newly established pan-denv rt-iipc ...	2016	27030492
determinants of arbovirus vertical transmission in mosquitoes.	vertical transmission (vt) and horizontal transmission (ht) of pathogens refer to parental and non-parental chains of host-to-host transmission. combining ht with vt enlarges considerably the range of ecological conditions in which a pathogen can persist, but the factors governing the relative frequency of each transmission mode are poorly understood for pathogens with mixed-mode transmission. elucidating these factors is particularly important for understanding the epidemiology of arthropod-bor ...	2016	27171170
physicians, primary caregivers and topical repellent: all under-utilised resources in stopping dengue virus transmission in affected households.	primary health care facilities frequently manage dengue cases on an ambulatory basis for the duration of the patient's illness. there is a great opportunity for specific messaging, aimed to reduce dengue virus (denv) transmission in and around the home, to be directly targeted toward this high-risk ambulatory patient group, as part of an integrated approach to dengue management. the extent however, to which physicians understand, and can themselves effectively communicate strategies to stop foca ...	2016	27164168
coeloconic sensilla on the antennae of the yellow fever mosquito, aedes aegypti (l.).		2016	5026452
dengue deaths: associated factors and length of hospital stay.	background. dengue continues to pose a public health problem globally. objective. to review factors associated with patients who died from dengue in trinidad. methods. a retrospective case note review of hospitalized patients who died during 2001 to 2010. results. a total of 23 cases were identified: 13 males, 10 females-12 east indians, 9 africans, and 2 unknown. more than half (n = 17) were over 40 years of age with 10 being over 60 years of age; three were children. a falling platelet count w ...	2016	27462472
tourniquet test for dengue diagnosis: systematic review and meta-analysis of diagnostic test accuracy.	dengue fever is a ubiquitous arboviral infection in tropical and sub-tropical regions, whose incidence has increased over recent decades. in the absence of a rapid point of care test, the clinical diagnosis of dengue is complex. the world health organisation has outlined diagnostic criteria for making the diagnosis of dengue infection, which includes the use of the tourniquet test (tt).	2016	27486661
afrocolombian ethnicity, a paradoxical protective factor against dengue.	dengue is a priority public health problem. during epidemics in cuba and haiti, ethnic african descendant population had lower risk of dengue, and the ethnic factor was proposed as a protective one.	2016	27821892
transcript abundance of photorhabdus insect-related (pir) toxin in manduca sexta and galleria mellonella infections.	in this study, we assessed pirab toxin transcription in photorhabdus luminescens laumondii (strain tt01) (enterobacteriaceae) by comparing mrna abundance under in vivo and in vitro conditions. in vivo assays considered both natural and forced infections with two lepidopteran hosts: galleria mellonella and manduca sexta. three portals of entry were utilized for the forced infection assays: (a) integument; (b) the digestive route (via mouth and anus); and (c) the tracheal route (via spiracles). we ...	2016	27690103
developmental pirna profiles of the invasive vector mosquito aedes albopictus.	in eukaryotic organisms, piwi-interacting rnas (pirnas) control the activities of mobile genetic elements and ensure genome maintenance. recent evidence indicates that pirnas are involved in multiple biological pathways, including transcriptional regulation of protein-coding genes, sex determination and even interactions between host and pathogens. aedes albopictus is a major invasive species that transmits a number of viral diseases in humans. ae. albopictus has the largest genome and the highe ...	2016	27686069
kap surveys and dengue control in colombia: disentangling the effect of sociodemographic factors using multiple correspondence analysis.	during the last few decades, several studies have analyzed and described knowledge, attitudes, and practices (kap) of populations regarding dengue. however, few studies have applied geometric data analytic techniques to generate indices from kap domains. results of such analyses have not been used to determine the potential effects of sociodemographic variables on the levels of kap. the objective was to determine the sociodemographic factors related to different levels of kap regarding dengue in ...	2016	27682141
spatial variations in dengue transmission in schools in thailand.	dengue is an important neglected tropical disease, with more than half of the world's population living in dengue endemic areas. good understanding of dengue transmission sites is a critical factor to implement effective vector control measures.	2016	27669170
n6-(2-hydroxyethyl)-adenosine exhibits insecticidal activity against plutella xylostella via adenosine receptors.	the diamondback moth, plutella xylostella, is one of the most important pests of cruciferous crops. we have earlier shown that n6-(2-hydroxyethyl)-adenosine (hea) exhibits insecticidal activity against p. xylostella. in the present study we investigated the possible mechanism of insecticidal action of hea on p. xylostella. hea is a derivative of adenosine, therefore, we speculated whether it acts via p. xylostella adenosine receptor (pxador). we used rnai approach to silence pxador gene and used ...	2016	27668428
evaluating the performance of infectious disease forecasts: a comparison of climate-driven and seasonal dengue forecasts for mexico.	dengue viruses, which infect millions of people per year worldwide, cause large epidemics that strain healthcare systems. despite diverse efforts to develop forecasting tools including autoregressive time series, climate-driven statistical, and mechanistic biological models, little work has been done to understand the contribution of different components to improved prediction. we developed a framework to assess and compare dengue forecasts produced from different types of models and evaluated t ...	2016	27665707
predicted structure of a minus-c obp from batocera horsfieldi (hope) suggests an intermediate structure in evolution of obps.	odorant binding proteins (obps) transport hydrophobic odorants from the environment to odorant receptors and play an important role in specific recognition of volatiles. here, we expressed and purified a minus-c obp, bhorobpm2, from batocera horsfieldi, a major pest of popolus, to determine its binding characteristics with 58 candidate volatiles using a fluorescence competition-binding assay. we showed that bhorobpm2 exhibited high binding affinity with chain volatiles and that ligands were sele ...	2016	27659921
potential of camellia sinensis proanthocyanidins-rich fraction for controlling malaria mosquito populations through disruption of larval development.	anopheles arabiensis and a. gambiae (sensu stricto) are the most prolific afrotropical malaria vectors. population control efforts of these two vectors have been hampered by extremely diverse larval breeding sites and widespread resistance to currently available insecticides. control of mosquito larval stages using bioactive compounds of plant origin has the potential to suppress vector populations leading to concomitant reduction in disease transmission rates. in this study, we evaluated the ef ...	2016	27660043
the whole genome sequence of the mediterranean fruit fly, ceratitis capitata (wiedemann), reveals insights into the biology and adaptive evolution of a highly invasive pest species.	the mediterranean fruit fly (medfly), ceratitis capitata, is a major destructive insect pest due to its broad host range, which includes hundreds of fruits and vegetables. it exhibits a unique ability to invade and adapt to ecological niches throughout tropical and subtropical regions of the world, though medfly infestations have been prevented and controlled by the sterile insect technique (sit) as part of integrated pest management programs (ipms). the genetic analysis and manipulation of medf ...	2016	27659211
sit 2.0: 21(st) century genetic technology for the screwworm sterile-insect program.	release of sterile insects, the sterile insect technique (sit), can be an extremely effective and precise method of pest control. a study in bmc biology from the new world screwworm sit program and others shows that modern genetic methods can provide major improvements even to this well-established and highly successful sit program.see research article: https://bmcbiol.biomedcentral.com/articles/10.1186/s12915-016-0296-8.	2016	27643991
gc content heterogeneity transition of conserved noncoding sequences occurred at the emergence of vertebrates.	conserved non-coding sequences (cnss) of eukaryotes are known to be significantly enriched in regulatory sequences. cnss of diverse lineages follow different patterns in abundance, sequence composition, and location. here, we report a thorough analysis of cnss in diverse groups of eukaryotes with respect to gc content heterogeneity. we examined 24 fungi, 19 invertebrates, and 12 non-mammalian vertebrates so as to find lineage specific features of cnss. we found that fungi and invertebrate cnss a ...	2016	28040773
non-mendelian dominant maternal effects caused by crispr/cas9 transgenic components in drosophila melanogaster.	the crispr/cas9 system has revolutionized genomic editing. the cas9 endonuclease targets genomic dna via an experimentally determined guide rna (grna). this results in a double-strand break at the target site. we generated transgenic drosophila melanogaster in which the crispr/cas9 system was used to target a gal4 transgene in vivo to our surprise, progeny whose genomes did not contain crispr/cas9 components were still capable of mutating gal4 sequences. we demonstrate this effect was caused by ...	2016	27638686
smshb, the sh2-containing adaptor protein b of schistosoma mansoni regulates venus kinase receptor signaling pathways.	venus kinase receptors (vkrs) are invertebrate receptor tyrosine kinases (rtks) formed by an extracellular venus fly trap (vft) ligand binding domain associated via a transmembrane domain with an intracellular tyrosine kinase (tk) domain. schistosoma mansoni vkrs, smvkr1 and smvkr2, are both implicated in reproductive activities of the parasite. in this work, we show that the sh2 domain-containing protein smshb is a partner of the phosphorylated form of smvkr1. expression of these proteins in xe ...	2016	27636711
lbd1 of vitellogenin receptor specifically binds to the female-specific storage protein sp1 via lbr1 and lbr3.	storage proteins are the major protein synthesized in the fat body, released into hemolymph and re-sequestered into the fat body before pupation in most insect species. storage proteins are important amino acid and nutrition resources during the non-feeding pupal period and play essential roles for the metamorphosis and oogenesis of insects. the sequestration of storage protein is a selective, specific receptor-mediated process. however, to date, the potential receptor mediating the sequestratio ...	2016	27637099
maintenance of host dna integrity in field-preserved mosquito (diptera: culicidae) blood meals for identification by dna barcoding.	determination of the interactions between hematophagous arthropods and their hosts is a necessary component to understanding the transmission dynamics of arthropod-vectored pathogens. current molecular methods to identify hosts of blood-fed arthropods require the preservation of host dna to serve as an amplification template. during transportation to the laboratory and storage prior to molecular analysis, genetic samples need to be protected from nucleases, and the degradation effects of hydroly ...	2016	27629021
agricultural chemicals: life changer for mosquito vectors in agricultural landscapes?	although many mosquito species develop within agricultural landscapes where they are potentially exposed to agricultural chemicals (fertilizers and pesticides), the effects of these chemicals on mosquito biology remain poorly understood. this study investigated the effects of sublethal concentrations of four agricultural chemicals on the life history traits of anopheles arabiensis and culex quinquefasciatus mosquitoes.	2016	27624456
silencing the olfactory co-receptor rferorco reduces the response to pheromones in the red palm weevil, rhynchophorus ferrugineus.	the red palm weevil (rpw, rhynchophorus ferrugineus), one of the most widespread of all invasive insect pest species, is a major cause of severe damage to economically important palm trees. rpw exhibits behaviors very similar to those of its sympatric species, the asian palm weevil (r. vulneratus), which is restricted geographically to the southern part of southeast asia. although efficient and sustainable control of these pests remains challenging, olfactory-system disruption has been proposed ...	2016	27606688
evolutionary dynamics of abundant stop codon readthrough.	translational stop codon readthrough emerged as a major regulatory mechanism affecting hundreds of genes in animal genomes, based on recent comparative genomics and ribosomal profiling evidence, but its evolutionary properties remain unknown. here, we leverage comparative genomic evidence across 21 anopheles mosquitoes to systematically annotate readthrough genes in the malaria vector anopheles gambiae, and to provide the first study of abundant readthrough evolution, by comparison with 20 droso ...	2016	27604222
the tomato spotted wilt virus genome is processed differentially in its plant host arachis hypogaea and its thrips vector frankliniella fusca.	thrips-transmitted tospoviruses are economically important viruses affecting a wide range of field and horticultural crops worldwide. tomato spotted wilt virus (tswv) is the type member of the tospovirus genus with a broad host range of more than 900 plant species. interactions between these viruses and their plant hosts and insect vectors via rnai pathways are likely a key determinant of pathogenicity. the current investigation, for the first time, compares biogenesis of small rnas between the ...	2016	27656190
rhodopsin 7-the unusual rhodopsin in drosophila.	rhodopsins are the major photopigments in the fruit fly drosophila melanogaster. drosophila express six well-characterized rhodopsins (rh1-rh6) with distinct absorption maxima and expression pattern. in 2000, when the drosophila genome was published, a novel rhodopsin gene was discovered: rhodopsin 7 (rh7). rh7 is highly conserved among the drosophila genus and is also found in other arthropods. phylogenetic trees based on protein sequences suggest that the seven drosophila rhodopsins cluster in ...	2016	27651995
spatiotemporal analysis of dengue fever in nepal from 2010 to 2014.	due to recent emergence, dengue is becoming one of the major public health problems in nepal. the numbers of reported dengue cases in general and the area with reported dengue cases are both continuously increasing in recent years. however, spatiotemporal patterns and clusters of dengue have not been investigated yet. this study aims to fill this gap by analyzing spatiotemporal patterns based on monthly surveillance data aggregated at district.	2016	27549095
the silencing of a 14-3-3ɛ homolog in tenebrio molitor leads to increased antimicrobial activity in hemocyte and reduces larval survivability.	the 14-3-3 family of phosphorylated serine-binding proteins acts as signaling molecules in biological processes such as metabolism, division, differentiation, autophagy, and apoptosis. herein, we report the requirement of 14-3-3ɛ isoform from tenebrio molitor (tm14-3-3ɛ) in the hemocyte antimicrobial activity. the tm14-3-3ɛ transcript is 771 nucleotides in length and encodes a polypeptide of 256 amino acid residues. the protein has the typical 14-3-3 domain, the nuclear export signal (nes) seque ...	2016	27556493
rural-urban comparisons of dengue seroprevalence in malaysia.	each year an estimated 390 million dengue infections occur worldwide. in malaysia, dengue is a growing public health concern but estimate of its disease burden remains uncertain. we compared the urban-rural difference of dengue seroprevalence and determined age-specific dengue seroprevalence in malaysia.	2016	27538986
genome-wide profiling of 24 hr diel rhythmicity in the water flea, daphnia pulex: network analysis reveals rhythmic gene expression and enhances functional gene annotation.	marine and freshwater zooplankton exhibit daily rhythmic patterns of behavior and physiology which may be regulated directly by the light:dark (ld) cycle and/or a molecular circadian clock. one of the best-studied zooplankton taxa, the freshwater crustacean daphnia, has a 24 h diel vertical migration (dvm) behavior whereby the organism travels up and down through the water column daily. dvm plays a critical role in resource tracking and the behavioral avoidance of predators and damaging ultravio ...	2016	27538446
the buzz about honey bee viruses.		2016	27537076
symptomatic dengue disease in five southeast asian countries: epidemiological evidence from a dengue vaccine trial.	dengue incidence has increased globally, but empirical burden estimates are scarce. prospective methods are best-able to capture all severities of disease. cyd14 was an observer-blinded dengue vaccine study conducted in children 2-14 years of age in indonesia, malaysia, thailand, the philippines, and vietnam. the control group received no vaccine and resembled a prospective, observational study. we calculated the rates of dengue according to different laboratory or clinical criteria to make infe ...	2016	27532617
multiple modes of action of the squamocin in the midgut cells of aedes aegypti larvae.	annonaceous acetogenins are botanical compounds with good potential for use as insecticides. in the vector, aedes aegypti (l.) (diptera: culicidae), squamocin (acetogenin) has been reported to be a larvicide and cytotoxic, but the modes of action of this molecule are still poorly understood. this study evaluated the changes in the cell morphology, and in the expression of genes, for autophagy (atg1 and atg8), for membrane ion transporter v-atpase, and for water channel aquaporin-4 (aqp4) in the ...	2016	27532504
nationwide study of factors associated with public's willingness to use home self-test kit for dengue fever in malaysia.	as there is no specific treatment for dengue, early detection and access to proper treatment may lower dengue fatality. therefore, having new techniques for the early detection of dengue fever, such as the use of dengue test kit, is vitally important. the aims of the study were: 1) identify factors associated with acceptance of a home self-test kit for dengue fever if the dengue test is available to the public and 2) find out the characteristics of the test kits that influence the use of the den ...	2016	27520825
traditional medicine practices among community members with diabetes mellitus in northern tanzania: an ethnomedical survey.	diabetes is a growing burden in sub-saharan africa where traditional medicines (tms) remain a primary form of healthcare in many settings. in tanzania, tms are frequently used to treat non-communicable diseases, yet little is known about tm practices for non-communicable diseases like diabetes.	2016	27514380
calculation of the average cost per case of dengue fever in mexico using a micro-costing approach.	the increasing burden of dengue fever (df) in the americas, and the current epidemic in previously unaffected countries, generate major costs for national healthcare systems. there is a need to quantify the average cost per df case. in mexico, few data are available on costs, despite df being endemic in some areas. extrapolations from studies in other countries may prove unreliable and are complicated by the two main mexican healthcare systems (the secretariat of health [ss] and the mexican soci ...	2016	27501146
surveillance, insecticide resistance and control of an invasive aedes aegypti (diptera: culicidae) population in california.	the invasion and subsequent establishment in california of aedes aegypti in 2013 has created new challenges for local mosquito abatement and vector control districts. studies were undertaken to identify effective and economical strategies to monitor the abundance and spread of this mosquito species as well as for its control. overall, bg sentinel (bgs) traps were found to be the most sensitive trap type to measure abundance and spread into new locations. autocidal-gravid-ovitraps (ago-b), when p ...	2016	27158450
dengue serotype immune-interactions and their consequences for vaccine impact predictions.	dengue is one of the most important and wide-spread viral infections affecting human populations. the last few decades have seen a dramatic increase in the global burden of dengue, with the virus now being endemic or near-endemic in over 100 countries world-wide. a recombinant tetravalent vaccine candidate (cyd-tdv) has recently completed phase iii clinical efficacy trials in south east asia and latin america and has been licensed for use in several countries. the trial results showed moderate-t ...	2016	27663790
virus-derived dna drives mosquito vector tolerance to arboviral infection.	mosquitoes develop long-lasting viral infections without substantial deleterious effects, despite high viral loads. this makes mosquitoes efficient vectors for emerging viral diseases with enormous burden on public health. how mosquitoes resist and/or tolerate these viruses is poorly understood. here we show that two species of aedes mosquitoes infected with two arboviruses from distinct families (dengue or chikungunya) generate a viral-derived dna (vdna) that is essential for mosquito survival ...	2016	27580708
transcriptional analysis of the adaptive digestive system of the migratory locust in response to plant defensive protease inhibitors.	herbivorous insects evolved adaptive mechanisms to compensate for the presence of plant defensive protease inhibitors (pi) in their food. the underlying regulatory mechanisms of these compensatory responses remain largely elusive. in the current study, we investigated the initiation of this adaptive response in the migratory locust, locusta migratoria, via microarray analysis of gut tissues. four hours after dietary uptake of pis, 114 and 150 transcripts were respectively found up- or downregula ...	2016	27581362
crygetter: a tool to automate retrieval and analysis of cry protein data.	for many years, the use of chemical agents to control crop pests has been degrading the environment, bringing problems to humans and all living things. an alternative to deal with the pests is the use of biopesticides, biological agents capable of controlling these harmful organisms. one kind of biopesticide is bacillus thuringiensis, a gram-positive bacterium that synthesizes a protein that, when ingested by the pests, kills them and does not harm other species.	2016	27578522
transcriptional differences between diapausing and non-diapausing d. montana females reared under the same photoperiod and temperature.	a wide range of insects living at higher latitudes enter diapause at the end of the warm season, which increases their chances of survival through harsh winter conditions. in this study we used rna sequencing to identify genes involved in adult reproductive diapause in a northern fly species, drosophila montana. both diapausing and non-diapausing flies were reared under a critical day length and temperature, where about half of the emerging females enter diapause enabling us to eliminate the eff ...	2016	27571415
the correlation between dengue incidence and diurnal ranges of temperature of colombo district, sri lanka 2005-2014.	meteorological factors affect dengue transmission. mechanisms of the way in which different diurnal temperatures, ranging around different mean temperatures, influence dengue transmission were published after 2011.	2016	27566717
developmental expression of ecdysone-related genes associated with metamorphic changes during midgut remodeling of silkworm bombyx mori (lepidoptera:bombycidae).	steroid hormone 20-hydroxyecdysone is known as the systemic regulators of insect cells; however, how to impact the fate and function of mature and stem cells is unclear. for the first time, we report ecdysone regulatory cascades in both mature midgut cell and stem cell fractions related to developmental events by using histological, immunohistochemical, biochemical and gene expression analysis methods. ecdysone receptor-b1 (ecr-b1) and ultraspiracle 1 (usp-1) mrnas were detected mainly in mature ...	2016	27620558
using wolbachia releases to estimate aedes aegypti (diptera: culicidae) population size and survival.	mosquitoes carrying the endosymbiont bacterium wolbachia have been deployed in field trials as a biological control intervention due to wolbachia effects on reducing transmission of arboviruses. we performed mark, release and recapture (mrr) experiments using wolbachia as an internal marker with daily collections with bg-traps during the first two weeks of releases in rio de janeiro, brazil. the mrr design allowed us to investigate two critical parameters that determine whether wolbachia would s ...	2016	27479050
dynamic spatiotemporal trends of imported dengue fever in australia.	dengue fever (df) epidemics in australia are caused by infected international travellers and confined to northern queensland where competent vectors exist. recent analyses suggest that global trade and climate change could lead to the re-establishment of ae. aegypti across the country and promote the spread of dengue nationally. this study aimed to describe the dynamic spatiotemporal trends of imported df cases and their origins, identify the current and potential future high-risk regions and lo ...	2016	27460696
opposing roles for dna replication initiator proteins orc1 and cdc6 in control of cyclin e gene transcription.	newly born cells either continue to proliferate or exit the cell division cycle. this decision involves delaying expression of cyclin e that promotes dna replication. orc1, the origin recognition complex (orc) large subunit, is inherited into newly born cells after it binds to condensing chromosomes during the preceding mitosis. we demonstrate that orc1 represses cyclin e gene (ccne1) transcription, an e2f1 activated gene that is also repressed by the retinoblastoma (rb) protein. orc1 binds to r ...	2016	27458800
additive effect of knockdown resistance mutations, s989p, v1016g and f1534c, in a heterozygous genotype conferring pyrethroid resistance in aedes aegypti in thailand.	mutation in the voltage-gated sodium channel gene that results in knockdown resistance (kdr), is a major mechanism of pyrethroid resistance in several mosquito species. in aedes aegypti, v1016g (occurring with and without s989p) and f1534c mutations are common and widely distributed throughout asia. the g1016 allele is known to be associated with resistance to type i and ii pyrethroids. the c1534 allele is primarily associated with resistance to type i pyrethroids and is known to be a recessive ...	2016	27460671
potential for extrinsic incubation temperature to alter interplay between transmission potential and mortality of dengue-infected aedes aegypti.	the extrinsic incubation period is a critical component in the assessment of arboviral transmission potential. it defines the time it takes for a mosquito to become infectious following exposure to an arbovirus. since this is a temporal process, the lifespan of a mosquito is intimately tied to the extrinsic incubation period and thus transmission potential of these viruses. temperature is a known effector of both vector competence (the ability of a vector to transmit a pathogen) and mosquito mor ...	2016	27478382