Publications

sweetgum: an ancient source of beneficial compounds with modern benefits.	sweetgum trees are large, deciduous trees found in asia and north america. sweetgum trees are important resources for medicinal and other beneficial compounds. many of the medicinal properties of sweetgum are derived from the resinous sap that exudes when the outer bark of the tree has been damaged. the sap, known as storax, has been used for centuries to treat common ailments such as skin problems, coughs, and ulcers. more recently, storax has proven to be a strong antimicrobial agent even agai ...	2015	26009686
nmr structure and function of helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm.	the cotton bollworm, helicoverpa armigera, has developed strong resistance to many insecticides. sterol carrier protein-2 (scp-2) is an important non-specific lipid transfer protein in insects and appears to be a potential new target. in order to elucidate the structure and function of helicoverpa armigera scp-2 (hascp-2), nmr spectroscopy, docking simulations, mutagenesis and bioassays were performed. hascp-2 composed of five α-helices and four stranded β-sheets. the folds of α-helices and β-sh ...	2015	26655641
variation in the ribosome interacting loop of the sec61α from giardia lamblia.	the interaction between the ribosome and the endoplasmic reticulum-located sec61 protein translocon is mediated through an arginine residue of sec61α, which is conserved in all prokaryotic and eukaryotic orthologues characterized to date. using in silico approaches we report that instead of arginine, this ribosome-interaction function is most likely discharged by a lysine residue in the protist giardia lamblia. this functional substitution of the r with a k in glsec61α may have taken place to ac ...	2015	26424409
evolutionary history of the photolyase/cryptochrome superfamily in eukaryotes.	photolyases and cryptochromes are evolutionarily related flavoproteins, which however perform distinct physiological functions. photolyases (phr) are evolutionarily ancient enzymes. they are activated by light and repair dna damage caused by uv radiation. although cryptochromes share structural similarity with dna photolyases, they lack dna repair activity. cryptochrome (cry) is one of the key elements of the circadian system in animals. in plants, cry acts as a blue light receptor to entrain ci ...	2015	26352435
no time to waste--the ethical challenges created by crispr: crispr/cas, being an efficient, simple, and cheap technology to edit the genome of any organism, raises many ethical and regulatory issues beyond the use to manipulate human germ line cells.		2015	26450575
the mutualistic side of wolbachia-isopod interactions: wolbachia mediated protection against pathogenic intracellular bacteria.	wolbachia is a vertically transmitted endosymbiont whose radiative success is mainly related to various host reproductive manipulations that led to consider this symbiont as a conflictual reproductive parasite. however, lately, some wolbachia have been shown to act as beneficial symbionts by protecting hosts against a broad range of parasites. still, this protection has been mostly demonstrated in artificial wolbachia-host associations between partners that did not co-evolved together. here, we ...	2015	26733946
ornamental exterior versus therapeutic interior of madagascar periwinkle (catharanthus roseus): the two faces of a versatile herb.	catharanthus roseus (l.) known as madagascar periwinkle (mp) is a legendary medicinal plant mostly because of possessing two invaluable antitumor terpenoid indole alkaloids (tias), vincristine and vinblastine. the plant has also high aesthetic value as an evergreen ornamental that yields prolific blooms of splendid colors. the plant possesses yet another unique characteristic as an amiable experimental host for the maintenance of the smallest bacteria found on earth, the phytoplasmas and spiropl ...	2015	25667940
arthropod vectors and disease transmission: translational aspects.		2015	26583380
bacterial infection and immune responses in lutzomyia longipalpis sand fly larvae midgut.	the midgut microbial community in insect vectors of disease is crucial for an effective immune response against infection with various human and animal pathogens. depending on the aspects of their development, insects can acquire microbes present in soil, water, and plants. sand flies are major vectors of leishmaniasis, and shown to harbor a wide variety of gram-negative and gram-positive bacteria. sand fly larval stages acquire microorganisms from the soil, and the abundance and distribution of ...	2015	26154607
sucupira as a potential plant for arthritis treatment and other diseases.	trees of the genus pterodon, commonly known as "sucupira-branca" or "faveira," are native to central brazil. the pterodon fruits are traditionally used in ethnomedicine as an infusion, in small doses, and at regular time intervals as an antirheumatic, anti-inflammatory, tonic, and depurative agent. the various compounds present in the pterodon class are, generally, water-insoluble and derived from the fusion of high-molecular weight pentacarbonate units. scientific research has shown that the ma ...	2015	26613051
heme signaling impacts global gene expression, immunity and dengue virus infectivity in aedes aegypti.	blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. heme is a pro-oxidant that influences a variety of cellular processes. we performed a global analysis of heme-regulated aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. we observed an iron- and reactive oxygen species (ros)-independent signaling induced by heme that comprised genes related to redox metabolis ...	2015	26275150
high throughput profiling of the cotton bollworm helicoverpa armigera immunotranscriptome during the fungal and bacterial infections.	innate immunity is essential in defending against invading pathogens in invertebrates. the cotton bollworm, helicoverpa armigera (hübner) is one of the most destructive lepidopteran pests, which causes enormous economic losses in agricultural production worldwide. the components of the immune system are largely unknown in this insect. the application of entomopathogens is considered as an alternative to the chemical insecticides for its control. however, few studies have focused on the molecular ...	2015	26001831
research on bacterial virulence in the developing countries.		2015	25713813
bilateral conjunctivitis in a returned traveller.		2015	25590460
of elic and evolution.		2015	25548134
insect-specific virus discovery: significance for the arbovirus community.	arthropod-borne viruses (arboviruses), especially those transmitted by mosquitoes, are a significant cause of morbidity and mortality in humans and animals worldwide. recent discoveries indicate that mosquitoes are naturally infected with a wide range of other viruses, many within taxa occupied by arboviruses that are considered insect-specific. over the past ten years there has been a dramatic increase in the literature describing novel insect-specific virus detection in mosquitoes, which has p ...	2015	26378568
insect-specific flaviviruses: a systematic review of their discovery, host range, mode of transmission, superinfection exclusion potential and genomic organization.	there has been a dramatic increase in the number of insect-specific flaviviruses (isfs) discovered in the last decade. historically, these viruses have generated limited interest due to their inability to infect vertebrate cells. this viewpoint has changed in recent years because some isfs have been shown to enhance or suppress the replication of medically important flaviviruses in co-infected mosquito cells. additionally, comparative studies between isfs and medically important flaviviruses can ...	2015	25866904
midgut immune responses induced by bacterial infection in the silkworm, bombyx mori.	insect gut epithelial cells produce reactive oxygen species (ros) and antimicrobial peptides (amps) to protect hosts from pathogenic microorganisms. in this study, we evaluate the pathogenicity of pseudomonas aeruginosa and bacillus bombysepticus in the silkworm, bombyx mori. survival curves show that b. bombysepticus is deadly when larval silkworms are infected orally. bacterial infection caused intestinal hydrogen peroxide (h2o2) and nitric oxide (no) levels to increase significantly by 8 and ...	2015	26465135
drosophila innate immunity: regional and functional specialization of prophenoloxidases.	the diversification of immune systems during evolution involves the expansion of particular gene families in given phyla. a better understanding of the metazoan immune system requires an analysis of the logic underlying such immune gene amplification. this analysis is now within reach due to the ease with which we can generate multiple mutations in an organism. in this paper, we analyze the contribution of the three drosophila prophenoloxidases (ppos) to host defense by generating single, double ...	2015	26437768
dengue virus infection of aedes aegypti requires a putative cysteine rich venom protein.	dengue virus (denv) is a mosquito-borne flavivirus that causes serious human disease and mortality worldwide. there is no specific antiviral therapy or vaccine for denv infection. alterations in gene expression during denv infection of the mosquito and the impact of these changes on virus infection are important events to investigate in hopes of creating new treatments and vaccines. we previously identified 203 genes that were ≥5-fold differentially upregulated during flavivirus infection of the ...	2015	26491875
an insight into the functional role of thioredoxin reductase, a selenoprotein, in maintaining normal native microbiota in the gulf coast tick (amblyomma maculatum).	tick selenoproteins have been associated with antioxidant activity in ticks. thioredoxin reductase (trxr), also a selenoprotein, belongs to the pyridine nucleotide-disulphide oxidoreductase family of proteins and is an important antioxidant. molecular interactions between native microbiota and tick hosts have barely been investigated to date. in this study, we determined the functional role of trxr in tick feeding and in maintenance of the native microbial community. trxr transcript levels remai ...	2015	26184979
nuclease tudor-sn is involved in tick dsrna-mediated rna interference and feeding but not in defense against flaviviral or anaplasma phagocytophilum rickettsial infection.	tudor staphylococcal nuclease (tudor-sn) and argonaute (ago) are conserved components of the basic rna interference (rnai) machinery with a variety of functions including immune response and gene regulation. the rnai machinery has been characterized in tick vectors of human and animal diseases but information is not available on the role of tudor-sn in tick rnai and other cellular processes. our hypothesis is that tick tudor-sn is part of the rnai machinery and may be involved in innate immune r ...	2015	26186700
movement of regulatory rna between animal cells.	recent studies suggest that rna can move from one cell to another and regulate genes through specific base-pairing. mechanisms that modify or select rna for secretion from a cell are unclear. secreted rna can be stable enough to be detected in the extracellular environment and can enter the cytosol of distant cells to regulate genes. mechanisms that import rna into the cytosol of an animal cell can enable uptake of rna from many sources including other organisms. this role of rna is akin to that ...	2015	26138457
infectious uveitis.	infectious uveitis is one of the most common and visually devastating causes of uveitis in the us and worldwide. this review provides a summary of the identification, treatment, and complications associated with certain forms of viral, bacterial, fungal, helminthic, and parasitic uveitis. in particular, this article reviews the literature on identification and treatment of acute retinal necrosis due to herpes simplex virus, varicella virus, and cytomegalovirus. while no agreed-upon treatment has ...	2015	26618074
abstracts from the 38th annual meeting of the society of general internal medicine.		2015	25869016
anopheles midgut epithelium evades human complement activity by capturing factor h from the blood meal.	hematophagous vectors strictly require ingesting blood from their hosts to complete their life cycles. exposure of the alimentary canal of these vectors to the host immune effectors necessitates efficient counteractive measures by hematophagous vectors. the anopheles mosquito transmitting the malaria parasite is an example of hematophagous vectors that within seconds can ingest human blood double its weight. the innate immune defense mechanisms, like the complement system, in the human blood sho ...	2015	25679788
developmental effects of ionizing radiation in aedes aegypti.		2015	5080436
prolyl oligopeptidase from the blood fluke schistosoma mansoni: from functional analysis to anti-schistosomal inhibitors.	blood flukes of the genus schistosoma cause schistosomiasis, a parasitic disease that infects over 240 million people worldwide, and for which there is a need to identify new targets for chemotherapeutic interventions. our research is focused on schistosoma mansoni prolyl oligopeptidase (smpop) from the serine peptidase family s9, which has not been investigated in detail in trematodes.	2015	26039195
[effects of 2 antimetabolites on the development of aedes aegypti larvae].		2015	4662010
paratransgenesis: a promising new strategy for mosquito vector control.	the three main mosquito genera, anopheles, aedes and culex, transmit respectively malaria, dengue and lymphatic filariasis. current mosquito control strategies have proved unsuccessful, and there still is a substantial number of morbidity and mortality from these diseases. genetic control methods have now arisen as promising alternative strategies, based on two approaches: the replacement of a vector population by disease-refractory mosquitoes and the release of mosquitoes carrying a lethal gene ...	2015	26104575
aerobic bacterial flora of biotic and abiotic compartments of a hyperendemic zoonotic cutaneous leishmaniasis (zcl) focus.	identification of the microflora of the sand fly gut and the environmental distribution of these bacteria are important components for paratransgenic control of leishmania transmission by sand flies.	2015	25630498
bacterial diversity associated with wild caught anopheles mosquitoes from dak nong province, vietnam using culture and dna fingerprint.	microbiota of anopheles midgut can modulate vector immunity and block plasmodium development. investigation on the bacterial biodiversity in anopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of africa. vietnam is an endemic country for both malaria and bancroftian filariasis whose parasitic agents can be transmitted by the same anopheles species. no information on the microbio ...	2015	25747513
the waal gene mutation compromised the inhabitation of enterobacter sp. ag1 in the mosquito gut environment.	the mosquito gut harbors a variety of bacteria that are dynamically associated with mosquitoes in various contexts. however, little is known about bacterial factors that affect bacterial inhabitation in the gut microbial community. enterobacter sp. ag1 is a predominant gram negative bacterium in the mosquito midgut.	2015	26306887
the dimerization state of the mammalian high mobility group protein at-hook 2 (hmga2).	the mammalian high mobility group protein at-hook 2 (hmga2) is a chromosomal architectural transcription factor involved in cell transformation and oncogenesis. it consists of three positively charged "at-hooks" and a negatively charged c-terminus. sequence analyses, circular dichroism experiments, and gel-filtration studies showed that hmga2, in the native state, does not have a defined secondary or tertiary structure. surprisingly, using combined approaches of 1-ethyl-3-(3-dimethylaminopropyl) ...	2015	26114780
oviposition responses of aedes mosquitoes to bacterial isolates from attractive bamboo infusions.	the mosquitoes aedes aegypti and aedes albopictus are vectors of pathogenic viruses that cause major human illnesses including dengue, yellow fever and chikungunya. both mosquito species are expanding their geographic distributions and now occur worldwide in temperate and tropical climates. collection of eggs in oviposition traps (ovitraps) is commonly used for monitoring and surveillance of container-inhabiting aedes populations by public health agencies charged with managing mosquito-transmitt ...	2015	26399712
the wolbachia wo bacteriophage proteome in the aedes albopictus c/wstr1 cell line: evidence for lytic activity?	wolbachia pipientis (rickettsiales), an obligate intracellular alphaproteobacterium in insects, manipulates host reproduction to maximize invasion of uninfected insect populations. modification of host population structure has potential applications for control of pest species, particularly if wolbachia can be maintained, manipulated, and genetically engineered in vitro. although wolbachia maintains an obligate mutualism with genome stability in nematodes, arthropods can be co-infected with dist ...	2015	26427709
nanoparticles as potential new generation broad spectrum antimicrobial agents.	the rapid emergence of antimicrobial resistant strains to conventional antimicrobial agents has complicated and prolonged infection treatment and increased mortality risk globally. furthermore, some of the conventional antimicrobial agents are unable to cross certain cell membranes thus, restricting treatment of intracellular pathogens. therefore, the disease-causing-organisms tend to persist in these cells. however, the emergence of nanoparticle (np) technology has come with the promising broad ...	2015	26329777
non-specific esterases in several organs of aedes aegypti (l.) during female adult life.		2015	4709581
the genome of diuraphis noxia, a global aphid pest of small grains.	the russian wheat aphid, diuraphis noxia kurdjumov, is one of the most important pests of small grains throughout the temperate regions of the world. this phytotoxic aphid causes severe systemic damage symptoms in wheat, barley, and other small grains as a direct result of the salivary proteins it injects into the plant while feeding.	2015	26044338
mutualism breakdown by amplification of wolbachia genes.	most insect species are associated with vertically transmitted endosymbionts. because of the mode of transmission, the fitness of these symbionts is dependent on the fitness of the hosts. therefore, these endosymbionts need to control their proliferation in order to minimize their cost for the host. the genetic bases and mechanisms of this regulation remain largely undetermined. the maternally inherited bacteria of the genus wolbachia are the most common endosymbionts of insects, providing some ...	2015	25668031
larvicidal activity of few select indigenous plants of north east india against disease vector mosquitoes (diptera: culicidae).	mosquitoes are the vectors of several life threatening diseases like dengue, malaria, japanese encephalitis and lymphatic filariasis, which are widely present in the north-eastern states of india. investigations on five local plants of north-east india, selected on the basis of their use by indigenous communities as fish poison, were carried out to study their mosquito larvicidal potential against anopheles stephensi (malaria vector), stegomyia aegypti (dengue vector) and culex quinquefasciatus ...	2015	25801251
[aedes aegypti larval infestation index and identification of awareness, attitudes and practices related to dengue in tire shops in atlántico, colombia].	objective to identify the awareness, attitudes and practices related to dengue in owners and workers of tire ships, as well as the levels of mosquito infestation in tire shops in atlántico department - colombia. methods we conducted a descriptive study. the variables were described as percentages and measures of central tendency and dispersion. index of larval infestation and containers were calculated in each of the municipalities studied. we visited and inspected 111 tire shops. 26.1 % (29/111 ...	2015	28453051
cold season mortality under natural conditions and subsequent hatching response of aedes (stegomyia) aegypti (diptera: culicidae) eggs in a subtropical city of argentina.	in temperate and subtropical regions, populations of aedes (stegomyia) aegypti (l.) survive unfavorable winter conditions in the egg stage. knowing their survival rates can be of great interest for the health authorities in charge of control activities. in this study, we analyzed the mortality of ae. aegypti eggs exposed to the cold season as well as their hatching patterns under laboratory conditions in the city of resistencia, chaco, argentina. the mortality rate was 48.6%. no statistically si ...	2015	26336247
pre-treatment of stegomyia aegypti mosquitoes with a sublethal dose of imidacloprid impairs behavioural avoidance induced by lemon oil and deet.	the present study was conducted to determine whether imidacloprid can impair the avoidance behaviour of the mosquito stegomyia aegypti. laboratory investigations using a t-maze apparatus showed that st. aegypti mosquitoes present long term avoidance behaviour when they are exposed to repetitive trials with lemon oil and deet. the present study tested the effect of a sublethal dose of imidacloprid on the avoidance behaviour of st. aegypti mosquitoes over a 48 h period. data suggest that 0.5 ng of ...	2015	25155403
new state records of mosquitoes for colorado.	the 1967 treatment of the mosquitoes of colorado by harmston and lawson and subsequent publications have recorded 46 culicid species from colorado. as part of a study to create an updated synopsis of the mosquitoes of colorado, adult trapping at numerous localities was conducted in colorado during the summers of 2013 and 2014. this review also included an examination of mosquito specimens in various relevant museum collections. aedes (ochlerotatus) niphadopsis and ae. (och.) spencerii spencerii ...	2015	26181688
insect-pathogen interactions: contribution of viral adaptation to the emergence of vector-borne diseases, the example of chikungunya.	the emergence or re-emergence of vector borne diseases represents a major public health problem. in general, therapeutic or prophylactic treatments along with vaccines are missing or inefficient, emphasizing the need for increased control of vector populations. understanding the interactions of human pathogens with their insect vectors will aid us in our understanding of viral emergence and the dynamics of these events. chikungunya virus (chikv) is a mosquito-borne virus that typically causes in ...	2015	29588001
[influence of the sodium chloride content of larval resting places on the sensitivity of aedes aegypti to d.d.t., in relation to the development of anai papillae].		2015	13536763
[action of development inhibitors on aedes aegypti l. mosquitoes and rhodnius prolixus stal. bugs. iv. the activity of precocenes].		2015	7024772
is there a risk of chikungunya transmission in canada?	an outbreak of chikungunya virus infection is currently underway in the caribbean, central america and south america, and autochthonous (local or indigenous) transmission has occurred in the southeastern united states. the mosquito species known to transmit chikungunya-aedes aegypti and aedes albopictus-are not known to reside in canada at this time. but how comfortable can we be that this situation will continue? here we explore four key conditions that must be met for transmission of chikungun ...	2015	29769915
[updating the larval habitats of aedes aegypti (diptera: culicidae) in camagüey, cuba].	several illnesses of vectorial origin have a great medical and veterinary relevance, due to the adaptations developed by the species involved in their transmission. to support preventive programs with updated ecological information of ae. aegypti populations, an entomological survey was carried out in three health areas of camagüey, cuba, during low and high rain seasons of 2013. for this, we assessed the type and number of positive containers, quantified larvae and pupae in positive containers, ...	2016	29465911
chemical composition, antioxidant, antimicrobial and insecticidal activities of essential oil from a moroccan endemic plant: bubonium imbricatum.	the chemical composition, antioxidant, antimicrobial and insecticidal properties of essential oil obtained by hydro-distillation from bubonium imbricatum cav. have been investigated. the essential oil of aerial parts was analyzed by gc-fid and gc-ms. a total of 63 compounds were identified representing more than 82% of the oil. the major components were cis-chrysanthenyl acetate (31.2%) and thymol isobutyrate (3.4%). the antioxidant activity was evaluated by the dpph and reducing power test. the ...	2016	30475515
lantana montevidensis essential oil: chemical composition and mosquito repellent activity against aedes aegypti.	the essential oil (eo) of lantana montevidensis (spreng.) briq. (l. sellowiana link & otto) was investigated for its chemical composition and mosquito repellent activity. the essential oil obtained by hydrodistillation of aerial plant parts was analyzed by gc-fid and gc-ms. the major constituents were p-elemene (22.0%), β-caryophyllene (20.1%), and germacrene d (9.4%). sesquiterpene hydrocarbons were present.in considerable quantities (78.9%) in the l. montevidensis eo, followed by oxygenated se ...	2016	30475514
toxicity of compounds isolated from white snakeroot (ageratina altissima) to adult and larval yellow fever mosquitoes (aedes aegypti).	botanicals have been the source for several classes of pesticides for mosquitoes. however, increasing resistance to these products and reduced numbers of choices necessitate the search for new active ingredients. ageratina altissima (l.) king & h. rob. or white snakeroot, is found throughout the eastern united states and contains known toxic compounds. we examine chromenes (benzopyrans) and benzofurans isolated fiom this plant for larvicidal and adulticidal activity against the dengue vector aed ...	2016	30475505
preparative capillary gc for characterization of five dracocephalum essential oils from mongolia, and their mosquito larvicidal activity.	the chemical composition and mosquito larvicidal and adulticidal activity of five essential oils (eos), dracocephalum ruyschiana l. (dr), d. foetidum bunge (df), d. moldavica l. (dm), d. fruticulosum steph. ex willd. (dfr) and d. peregrinum l. (dp) were evaluated. simultaneous gc-fid and gc-ms analyses revealed in the eos of dr, df and dm an unidentified compound (1) ([m'122], 5.4%, 57.9% and 74.0%, respectively). therefore, we aimed to isolate compound 1 using preparative capillary gc (pcgc) co ...	2016	30549618
chemical composition and biting deterrent activity of essential oil of tagetes patula (marigold) against aedes aegypti.	the hydrodistilled leaf essential oil (eo) of tagetes patula l. was analyzed by gc-fid and gc-ms. the main components of the oil were characterized as caryophyllene oxide (18.4%), p-caryophyllene (18.0%) and spathulenol (9.1%). the eo was screened for its biting deterrent activity against aedes aegypti l. using the in vitro k&d module system. t. patula eo exhibited good biting deterrent activity. the results suggest that these sesquiterpenes may contribute to biting deterrent activity, but the r ...	2016	30549616
essential oil composition of pimpinella cypria and its insecticidal, cytotoxic, and antimicrobial activity.	a water-distilled essential oil (e) from the aerial parts of pimpinella cypria boiss. (apiaceae), an endemic species in northern cyprus, was analyzed by gc- fid and gc-ms. forty-five compounds were identified in the oil, which comprised 81.7% of the total composition. the compound classes in the oil were oxygenated sesquiterpenes (33.9%), sesquiterpenes (22.0%), monoterpenes (11.4%), oxygenated monoterpenes (2.6%), and phenylpropanoids (7.5%). the main components of the oil were (z)-β-farnesene ...	2016	30549615
11-hydroxy-2,4-cycloeudesmane from the leaf oil of juglans regia and evaluation of its larvicidal activity.	leaves of juglans regia l. collected from two different locations [adana (a) and ankara (b)] from turkey were subjected to hydrodistillation in a clevenger type apparatus to yield the essential oils (eos). gc/ms and gc-fid analyses revealed that the a eo was rich in thymol (23.1%), while caryophyllene oxide (33.8%) was found as the main constituent of b eo. both contained p-eudesmol (1.4% - 9.5 %), (e)-geranyl acetone (3.7% - 5.8%) and the eudesmane type constituent juglaterpene a (3.1% - 11.0%) ...	2016	30549590
repellent and insecticidal efficacy of a combination of dinotefuran, pyriproxyfen and permethrin (vectra® 3d) against culex pipiens in dogs.	culex pipiens is an important vector of pathogens of substantial medical and veterinary importance such as dirofilaria immitis and dirofilaria repens or the west nile virus. the control of these mosquitoes is therefore essential to control the transmission of mosquito-borne agents to humans and animals. a combination of dinotefuran, permethrin and pyriproxyfen (vectra® 3d) has already shown its efficacy against aedes aegypti. the aim of this study was to confirm the efficacy of this combination ...	2016	29988200
[construction of aaldv-3 mediated mirrna sponge delivery system and its effect on cell and larva of aedes aegypti].	micrornas (mirnas) not only play the key roles in regulation of the growth and development of mosquito, but also has an important function in interaction between the pathogen and vector. so, mirnas can be used as the molecular target for development of an alternative method for mosquito and mosquito-borne disease control. however, an effective delivery system is still need. mosquito densovirus have the potential for vector control as transducing agents to express foreign toxins or small interfer ...	2016	29979549
bioefficacy of ecbolin a and ecbolin b isolated from ecbolium viride (forsk.) alston on dengue vector aedes aegypti l. (diptera: culicidae).	ecbolin a and ecbolin b were isolated from ethyl acetate extract of ecbolium viride (forsk.) alston root and evaluated for larvicidal and growth disturbance activities against aedes aegypti l. (diptera: culicidae). for larvicidal activity, the third instar larvae of a. aegypti were exposed to different concentrations viz., 1.0, 2.5, 5.0 and 10 ppm for each compound. among the two compounds screened, ecbolin b recorded highest larvicidal activity with lc50 and lc90 values of 0.70 and 1.42 ppm, re ...	2016	29988190
effect of gamma radiation on life history traits of aedes aegypti (l.).	aedes aegypti is an important vector for dengue and dengue hemorrhagic fever. considering its medical importance and its relevance as a model system, this study was undertaken to evaluate the impact of different doses of gamma radiation for three generations of a. aegypti. two to three days old virgin males of a. aegypti were irradiated with 15 doses of gamma radiation, ranging from 1 to 50 gy and were immediately mass mated with the same aged virgin females. observations were made for changes o ...	2016	29988174
[identification and expression of the yellow gene family in aedes aegypti].	to identify the yellow family genes in aedes aegypti and analyze the gene structure, phylogenetic evolution and their expression at various developmental stages and in different tissues.	2016	30120914
[the filaria dipetalonema dessetae: phenomena of regulation and parasite yield in the aedes vector].	numerical observations were made on the various steps of the cycle of dipetalonema dessetae in aedes aegypti: -- ingestion of microfilariae: the numbers of the ingested microfilariae correspond to a negative binomial distribution, but the over-dispersion is less important than in the bancroft's filaria as observed by pichon; in this last case the microfilariae are thicker and thus are more slowed in the capillari; -- crossing of the stomach wall: the "limitation" is caused by a precociously esta ...	2016	754621
[aedes aegypti eradication campaign in chile].		2016	12977855
[increased artificial resistance to ddt in adult aedes aegypti by contact of larvae with the insecticide].		2016	13585041
[findings on the biology of culicidae in hanoï (tonkin, north vietnam). i. comparative larval development of various strains of aedes albopictus, aedes aegypti, culex fatigans & armigeres obturans].		2016	13559861
[limits of the possibilities of acquisition of resistance to ddt of a strain of aedes aegypti subjected to known larval selection for 11 successive generations].		2016	13886805
[the ability of sumah virus of the ukuniemi group to reproduce in aedes aegypti mosquitoes].		2016	5141615
dengue fever outbreaks in eritrea, 2005-2015: a case for strengthening surveillance, control and reporting.	the geographic distribution and burden of dengue is increasing globally. this study aims to evaluate dengue outbreaks and to substantiate the need for strengthened surveillance, reporting and control in eritrea.	2016	29202065
phylogenetic analysis of aedes aegypti based on mitochondrial nd4 gene sequences in almadinah, saudi arabia.	aedes aegypti is the main vector of the yellow fever and dengue virus. this mosquito has become the major indirect cause of morbidity and mortality of the human worldwide. dengue virus activity has been reported recently in the western areas of saudi arabia. there is no vaccine for dengue virus until now, and the control of the disease depends on the control of the vector.	2016	28959327
knowledge, attitudes, and practices regarding dengue infection among public sector healthcare providers in machala, ecuador.	dengue fever is a rapidly emerging infection throughout the tropics and subtropics with extensive public health burden. adequate training of healthcare providers is crucial to reducing infection incidence through patient education and collaboration with public health authorities. we examined how public sector healthcare providers in a dengue-endemic region of ecuador view and manage dengue infections, with a focus on the 2009 world health organization (who) dengue guidelines.	2016	28883952
optimal repellent usage to combat dengue fever.	dengue fever is one of the most important vector-borne diseases. it is transmitted by aedes stegomyia aegypti, and one of the most effective strategies to combat the disease is the reduction of exposure to bites of these mosquitoes. in this paper, we present a game-theoretical model in which individuals choose their own level of protection against mosquito bites in order to maximize their own benefits, effectively balancing the cost of protection and the risk of contracting the dengue fever. we ...	2016	27142427
[effect of pyriproxyfen for the aedes (s) aegypti control (diptera: culicidae) in strains with various degrees of temephos resistance].	the continuous use of the organophosphate temephos for aedes (stegomyia) aegypti (linnaeus, 1762) has prompted the emergence of resistance to this product in several countries. pyriproxyfen is an analogue of the juvenile hormone recommended by the world health organization as one of the alternative regulators of the dengue vector in drinking waters.	2016	23437553
comparison of two detailed models of aedes aegypti population dynamics.	the success of control programs for mosquito borne diseases can be enhanced by crucial information provided by models of the mosquito populations. models, however, can differ in their structure, complexity and biological assumptions, and these differences impact their predictions. unfortunately, it is typically difficult to determine why two complex models make different predictions because we lack structured side-by-side comparisons of models using comparable parameterization. here we present a ...	2016	28462011
whatch'em: a weather-driven energy balance model for determining water height and temperature in container habitats for aedes aegypti.	the mosquito virus vector aedes (ae.) aegypti exploits a wide range of containers as sites for egg laying and development of the immature life stages, yet the approaches for modeling meteorologically sensitive container water dynamics have been limited. this study introduces the water height and temperature in container habitats energy model (whatch'em), a state-of-the-science, physically based energy balance model of water height and temperature in containers that may serve as development sites ...	2016	29123363
annotation of the asian citrus psyllid genome reveals a reduced innate immune system.	citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as huanglongbing. the citrus greening bacteria, candidatus liberibacter asiaticus (clas), is a persistent propagative pathogen transmitted by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). hemipterans characterized to date lack a number of insect immune genes, including those associated with the imd pathway targeting gram-negative bacteria. the d. citri draft ...	2016	27965582
disruption of phenylalanine hydroxylase reduces adult lifespan and fecundity, and impairs embryonic development in parthenogenetic pea aphids.	phenylalanine hydroxylase (pah) is a key tyrosine-biosynthetic enzyme involved in neurological and melanin-associated physiological processes. despite extensive investigations in holometabolous insects, a pah contribution to insect embryonic development has never been demonstrated. here, we have characterized, for the first time, the pah gene in a hemimetabolous insect, the aphid acyrthosiphon pisum. phylogenetic and sequence analyses confirmed that appah is closely related to metazoan pah, exhi ...	2016	27694983
convergent evolution of trna gene targeting preferences in compact genomes.	in gene-dense genomes, mobile elements are confronted with highly selective pressure to amplify without causing excessive damage to the host. the targeting of trna genes as potentially safe integration sites has been developed by retrotransposons in various organisms such as the social amoeba dictyostelium discoideum and the yeast saccharomyces cerevisiae. in d. discoideum, trna gene-targeting retrotransposons have expanded to approximately 3 % of the genome. recently obtained genome sequences o ...	2016	27583033
small rna regulators of plant-hemipteran interactions: micromanagers with versatile roles.	non-coding small rnas (srnas) in plants have important roles in regulating biological processes, including development, reproduction, and stress responses. recent research indicates significant roles for srna-mediated gene silencing during plant-hemipteran interactions that involve all three of these biological processes. plant responses to hemipteran feeding are determined by changes in the host transcriptome that appear to be fine-tuned by srnas. the role of srna in plant defense responses is ...	2016	27625654
novel insights into insect-microbe interactions-role of epigenomics and small rnas.	it has become increasingly clear that microbes form close associations with the vast majority of animal species, especially insects. in fact, an array of diverse microbes is known to form shared metabolic pathways with their insect hosts. a growing area of research in insect-microbe interactions, notably for hemipteran insects and their mutualistic symbionts, is to elucidate the regulation of this inter-domain metabolism. this review examines two new emerging mechanisms of gene regulation and th ...	2016	27540386
heat sensitivity of wmel wolbachia during aedes aegypti development.	the wmel strain of wolbachia bacteria is known to prevent dengue and zika virus transmission in the mosquito vector aedes aegypti. accordingly, the release of wmel-infected a. aegypti in endemic regions has been recommended by the world health organization as a potential strategy for controlling dengue and zika outbreaks. however, the utility of this approach could be limited if high temperatures in the aquatic habitats where a. aegypti develop are detrimental to wolbachia. we exposed wmel-infec ...	2016	27459519
de novo transcriptome assembly of the grapevine phylloxera allows identification of genes differentially expressed between leaf- and root-feeding forms.	grapevine phylloxera, an insect related to true aphids, is a major historic pest of viticulture only controlled through the selection of resistant rootstocks or through quarantine regulations where grapevine is cultivated own-rooted. transcriptomic data could help understand the bases of its original life-traits, including a striking case of polyphenism, with forms feeding on roots and forms feeding in leaf-galls. comparisons with true aphids (for which complete genomes have been sequenced) shou ...	2016	26968158
detection of dengue, west nile virus, rickettsiosis and leptospirosis by a new real-time pcr strategy.	dengue virus (denv) infection causes sudden fever along with several nonspecific signs and symptoms and in severe cases, death. denv is transmitted to people by aedes aegypti and ae. albopictus mosquitoes, whose populations increase during rainy season. west nile virus (wnv), rickettsia spp. and leptospira spp. are fever-causing pathogens that share many of the initial symptoms of denv infection and also thrive in the rainy season. outbreaks in some regions may be due to any of these pathogens t ...	2016	27350908
zika virus: an update on epidemiology, pathology, molecular biology, and animal model.	zika virus (zikv) was first described in 1947, and became a health emergency problem in 2016 when its association with fetal microcephaly cases was confirmed by centers for disease control and prevention (cdc) in the united states. to date, zikv infection has been documented in 66 countries. zikv is recognized as a neurotropic virus and numerous diseases manifested in multiple neurological disorders have been described, mainly in countries that have been exposed to zikv after the 2007 outbreak i ...	2016	27124623
magnitude of neglected tropical diseases in indonesia at postmillennium development goals era.	the world will enter the postmillennium development goals 2015 era. the achievements of the millennium development goals (mdgs) as a global development target need to be evaluated. a sustainable new reasonable target is important for neglected tropical diseases (ntd) elimination in indonesia. this review describes the ntd situation in indonesia and highlights problems beneath the ntd transmission. multidisciplinary approach is a promising strategy to help the marginalized people.	2016	27190525
the untapped cell biology of neglected tropical diseases.	the world health organization lists a constellation of 17 tropical diseases that afflict approximately one in six individuals on the planet and, until recently, few resources have been devoted to the treatment and eradication of those diseases. they are often referred to as the diseases of the "bottom billion," because they are most prevalent among the poorest individuals in impoverished tropical nations. however, the few studies that have been performed reveal an extraordinary world of molecula ...	2016	26915691
structure and functional diversity of gcn5-related n-acetyltransferases (gnat).	general control non-repressible 5 (gcn5)-related n-acetyltransferases (gnat) catalyze the transfer of an acyl moiety from acyl coenzyme a (acyl-coa) to a diverse group of substrates and are widely distributed in all domains of life. this review of the currently available data acquired on gnat enzymes by a combination of structural, mutagenesis and kinetic methods summarizes the key similarities and differences between several distinctly different families within the gnat superfamily, with an emp ...	2016	27367672
overview of zika virus (zikv) infection in regards to the brazilian epidemic.	zika virus (zikv), a mosquito-borne flavivirus, belongs to the flaviviridae family, genus flavivirus. zikv was initially isolated in 1947 from a sentinel monkey in the zika forest, uganda. little clinical importance was attributed to zikv, once only few symptomatic cases were reported in some african and southeast asiatic countries. this situation changed in 2007, when a large outbreak was registered on the yap island, micronesia, caused by the asian zikv lineage. between 2013 and 2014, zikv spr ...	2016	27143174
zika: the origin and spread of a mosquito-borne virus.	to describe the temporal and geographical distribution of zika virus infection and associated neurological disorders, from 1947 to 1 february 2016, when zika became a public health emergency of international concern (pheic).	2016	27708473
persistent infection by wolbachia walbb has no effect on composition of the gut microbiota in adult female anopheles stephensi.	the bacteria in the midgut of anopheles stephensi adult females from laboratory colonies were studied by sequencing the v4 region of 16s rrna genes, with respect to three experimental factors: stable or cured wolbachia infection; sugar or blood diet; and age. proteobacteria and bacteroidetes dominated the community [>90% of operational taxonomic units (otus)]; most taxa were in the classes flavobacteriia, gammaproteobacteria, and alphaproteobacteria, and were assigned to elizabethkingia (46.9%), ...	2016	27708633
functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts.	as both larvae and adults, mosquitoes encounter a barrage of immune insults, ranging from microbe-rich communities in larval habitats to ingested blood-borne pathogens in adult blood meals. given that mosquito adults have evolved an efficient means of eliminating infections in their hemocoel (body cavity) via the coordinated action of their immune and circulatory systems, the goal of the present study was to determine whether such functional integration is also present in larvae.	2016	27643786
mosquito c-type lectins maintain gut microbiome homeostasis.	the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ...	2016	27170846
open-air biowarfare testing and the evolution of values.	the united states and the united kingdom ended outdoor biological warfare testing in populated areas nearly half a century ago. yet, the conduct, health effects, and propriety of those tests remain controversial. the varied views reflect the limits of currently available test information and evolving societal values on research involving human subjects. western political culture has changed since the early days of the american and british testing programs. people have become less reluctant to qu ...	2016	27564984
symbiosis with francisella tularensis provides resistance to pathogens in the silkworm.	francisella tularensis, the causative agent of tularemia, is a highly virulent facultative intracellular pathogen found in a wide range of animals, including arthropods, and environments. this bacterium has been known for over 100 years, but the lifestyle of f. tularensis in natural reservoirs remains largely unknown. thus, we established a novel natural host model for f. tularensis using the silkworm (bombyx mori), which is an insect model for infection by pathogens. f. tularensis established a ...	2016	27507264
advances and limitations of disease biogeography using ecological niche modeling.	mapping disease transmission risk is crucial in public and animal health for evidence based decision-making. ecology and epidemiology are highly related disciplines that may contribute to improvements in mapping disease, which can be used to answer health related questions. ecological niche modeling is increasingly used for understanding the biogeography of diseases in plants, animals, and humans. however, epidemiological applications of niche modeling approaches for disease mapping can fail to ...	2016	27547199
diversity, evolution and medical applications of insect antimicrobial peptides.	antimicrobial peptides (amps) are short proteins with antimicrobial activity. a large portion of known amps originate from insects, and the number and diversity of these molecules in different species varies considerably. insect amps represent a potential source of alternative antibiotics to address the limitation of current antibiotics, which has been caused by the emergence and spread of multidrug-resistant pathogens. to get more insight into amps, we investigated the diversity and evolution o ...	2016	27160593
histopathology of aedes aegypti (diptera: culicidae) larvae parasitized by reesimermis nielseni (nematoda: mermithidae).		2016	4761359
esterase polymorphism in aedes aegypti: the genetics and k m values of electrophoretically heterogeneous forms.		2016	5038250
frequency of blood feeding in the mosquito aedes aegypti.		2016	4937213
systemically and cutaneously distributed ectoparasiticides: a review of the efficacy against ticks and fleas on dogs.	acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. both externally and systemically distributed treatments have benefits and weaknesses in potentially preventing pathogen transmission by these arthropod vectors.four general properti ...	2016	27502490