Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| role of aedes aegypti and aedes albopictus during the 2011 dengue fever epidemics in hanoi, vietnam. | to record the human cases of dengue fever (df) and investigate the aedes mosquito species circulating during the hanoi 2011 df epidemics. | 2015 | 26276285 |
| different serotypes of dengue virus (denv) imported by polish travellers from dengue endemic areas to poland. | dengue viruses are the most widespread arboviruses (transmitted mainly by aedes aegypti and ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. there are four dengue virus serotypes: denv-1, denv-2, denv-3, and denv-4. subsequent infections increase the risk of developing severe dengue fever. | 2015 | 26119675 |
| detection of dengue virus in individual aedes aegypti mosquitoes in delhi, india. | delhi, the capital city of india, has so far witnessed several outbreaks of dengue fever since 1967 (last one reported in 2013). improved virological and entomological surveillance are the only tools that can help in prevention of dengue as well as in the development of dengue control programmes. the aim of the study was to conduct a prospective field study to detect dengue virus in adult aedes aegypti mosquitoes collected from various localities represented by different socioeconomic groups in ... | 2015 | 26119544 |
| [vertical transmission of dengue virus in aedes aegypti, peru]. | 2015 | 26102125 | |
| [dengue in peru: a quarter century after its reemergence]. | a health problem each time more frequent and dispersed in tropical and subtropical areas of the world, including peru where it entered in 1990, is dengue. it is produced by the dengue virus with four serotypes and transmitted by aedes aegypti, a vector that coexists with humans and whose presence is favored by deficient sanitary, social and economic conditions. manifestations of severe forms of the disease such as shock and bleeding, are related to the frequent co-circulation of the four serotyp ... | 2015 | 26102118 |
| green-synthesized silver nanoparticles as a novel control tool against dengue virus (den-2) and its primary vector aedes aegypti. | dengue is an arthropod-borne viral infection mainly vectored through the bite of aedes mosquitoes. recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depends on effective vector control measures. in this study, we proposed the green-synthesis of silver nanoparticles (agnp) as a novel and eff ... | 2015 | 26063530 |
| development and validation of an arthropod maceration protocol for zoonotic pathogen detection in mosquitoes and fleas. | arthropod-borne diseases remain a pressing international public health concern. while progress has been made in the rapid detection of arthropod-borne pathogens via quantitative real-time (qpcr), or even hand-held detection devices, a simple and robust maceration and nucleic acid extraction method is necessary to implement biosurveillance capabilities. in this study, a comparison of maceration techniques using five types of beads followed by nucleic acid extraction and detection were tested usin ... | 2015 | 26047188 |
| dengue virus detection in aedes aegypti larvae from southeastern brazil. | the transmission of dengue, the most important arthropod-borne viral disease in brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its aedes vectors. in the present study, we mapped dengue vectors in ouro preto and ouro branco, minas gerais, by installing ovitraps in 32 public schools. the traps were examined monthly between september, 2011 through july, 2012 and november, 2012 to april, 2013. the larvae were reared until the fourth stadiu ... | 2015 | 26047186 |
| sero-prevalence of dengue infections in the kassala state in the eastern part of the sudan in 2011. | dengue fever is a vector-borne disease that is transmitted to humans by infected aedes aegypti mosquitoes. the eastern part of the sudan is one of the regions that is affected by dengue virus circulation. in this study, we estimated the prevalence of dengue infections in the kassala state in the eastern part of the sudan. the study objective was to estimate the sero-prevalence of dengue igg/igm antibodies in the kassala locality in 2011. this was a cross sectional community-based study that util ... | 2015 | 25975993 |
| natural transovarial transmission of dengue virus 4 in aedes aegypti from cuiabá, state of mato grosso, brazil. | dengue is the most prevalent arboviral disease in tropical areas. in mato grosso, outbreaks are reported every year, but studies on dengue in this state are scarce. | 2015 | 25860459 |
| susceptibility of indigenous and transplanted mosquito spp. to dengue virus in japan. | dengue fever, an acute, mosquito-borne, febrile illness caused by flavivirus spp., is a problem in africa, south and southeast asia, latin america, and the caribbean. a dengue outbreak occurred after nearly 70 years of absence or no detection, and then 158 autochthonous cases occurred in japan from august to october 15, 2014. the most competent mosquito vectors for dengue virus transmission were aedes aegypti and a. albopictus. since a. albopictus is widely distributed across japan and a. aegypt ... | 2015 | 25766611 |
| comparative efficacy of existing surveillance tools for aedes aegypti in western kenya. | all traditional surveillance techniques for aedes aegypti have been developed for the cosmopolitan domestic subspecies ae. aegypti aegypti, and not the sylvatic subspecies, ae. aegypti formosus. the predominant form in western kenya is ae. aegypti formosus that is rarely associated with human habitations but is linked to transmission of sylvatic dengue virus strains. we compared five surveillance methods for their effectiveness in sampling ae. aegypti formosus with the goal of determining a sust ... | 2015 | 26611965 |
| natural vertical transmission of dengue viruses in aedes aegypti in selected sites in cebu city, philippines. | we attempted to determine the vertical transmission of dengue virus (denv) in aedes aegypti in selected sites in cebu city, philippines. mosquito sub-adults were collected monthly from households and the field during the wet-dry-wet season from november, 2011 to july, 2012 and were laboratory-reared to adults. viral rna extracts in mosquitoes were assayed by hemi-nested rt-pcr. results showed that 62 (36.26%; n=679) out of 171 mosquito pools (n=2,871) were denv+. the minimum infection rate (mir) ... | 2015 | 26611963 |
| innate antiviral immunity against dengue virus. | dengue virus (denv), the most prevalent mosquito-borne viral diseases in humans worldwide, causes dengue fever, a mild form of the disease, as well as dengue hemorrhagic fever/dengue shock syndrome, a more severe form which can be life-threatening. the four serotypes of denv (denv1-4) are positive-sense, single stranded rna virus belonging to the flaviviridae family and are transmitted by aedes aegypti and aedes albopictus mosquitoes. together, they are estimated to cause almost 100 million symp ... | 2015 | 26559229 |
| [estimation of aedes aegypti (l.) (diptera: culicidae) productivity in households and public spaces in a dengue endemic city in colombia]. | aedes aegypti is a vector for the dengue virus in colombia. its productivity can be estimated using pupal counts. | 2015 | 26535548 |
| essential oils from zanthoxylum fagara wild lime, ruta chalepensis l. and thymus vulgaris l.: composition and activity against aedes aegypti larvae. | the dengue virus is transmitted by aedes aegypti. several plants are used to control this mosquito. in the present study the chemical composition of the essential oils of ruta chalepensis, zanthoxylum fagara and thymus vulgaris were analyzed, and their activities against larvae of two a. aegypti populations were evaluated. the major compounds found in t. vulgaris were thymol and -cymene at 39.8% and 30.5%, respectively, with the major components being oxygenated monoterpenes and monoterpene hyd ... | 2015 | 26525020 |
| dengue fever and dengue hemorrhagic fever in adults. | dengue fever and dengue hemorrhagic fever are re-emerging diseases that are endemic in the tropics. the global prevalence of dengue cases has increased in south-east asia, africa, the western pacific, and the americas. the increasingly widespread distribution and the rising incidence of dengue virus infections are related to increased distribution of aedes aegypti, an increasingly urban population, and increasing air travel. several southeast asian countries show that the age of the reported den ... | 2015 | 26506734 |
| a role for vector control in dengue vaccine programs. | development and deployment of a successful dengue virus (denv) vaccine has confounded research and pharmaceutical entities owing to the complex nature of denv immunity and concerns over exacerbating the risk of denv hemorrhagic fever (dhf) as a consequence of vaccination. thus, consensus is growing that a combination of mitigation strategies will be needed for denv to be successfully controlled, likely involving some form of vector control to enhance a vaccine program. we present here a determin ... | 2015 | 26478199 |
| chikungunya: here today, where tomorrow? | 2015 | 25576501 | |
| a mosquito survey of the twin-island caribbean nation of saint kitts and nevis, 2010. | adult mosquito surveys of saint kitts and nevis (skn) were performed in the dry season (march 16-23, 2010) in saint kitts, and the rainy season (october 18-25, 2010) in skn. biogents (bg) sentinel traps were set with co₂and bg lure in urban, rural, mangrove, and dry forest habitats. mosquitoes were identified to species, and reverse transcription-polymerase chain reaction was performed on potential vector species for dengue virus (denv), chikungunya virus (chikv), and west nile virus (wnv). the ... | 2015 | 26675458 |
| chikungunya. | chikungunya is an acute viral disease characterised by fever and painful arthralgia. the arthritic symptoms associated with chikungunya can be debilitating and may persist for months or even years in some patients. severe neurological complications such as encephalitis have also been reported during recent large outbreaks. the disease is caused by chikungunya virus (chikv), a mosquito-borne alphavirus from the togaviridae family, which has recently emerged to become one of the most important exo ... | 2015 | 26601450 |
| historical inability to control aedes aegypti as a main contributor of fast dispersal of chikungunya outbreaks in latin america. | the arrival of chikungunya fever (chikf) in latin american countries has been expected to trigger epidemics and challenge health systems. historically considered as dengue-endemic countries, abundant aedes aegypti populations make this region highly vulnerable to chikungunya virus (chikv) circulation. this review describes the current dengue and chikf epidemiological situations, as well as the role of uncontrolled ae. aegypti and aedes albopictus vectors in spreading the emerging chikv. comments ... | 2015 | 26518229 |
| arbovirus surveillance and first report of chikungunya virus in wild populations of aedes aegypti from guerrero, mexico. | we carried out dengue (denv) and chikungunya virus (chikv) surveillance in wild populations of aedes aegypti from guerrero, mexico, from 2012 to 2014 following a standard national protocol of the mexican dengue control program. a total of 284 pools (15-30 specimens/pool) of female mosquitoes were tested with real-time reverse transcriptase-polymerase chain reaction to detect denv and chikv. we report for the 1st time the detection of chikv from field-collected mosquitoes at acapulco and juchitán ... | 2015 | 26375910 |
| assessing carbon dioxide and synthetic lure-baited traps for dengue and chikungunya vector surveillance (3). | the aedes mosquito vectors of dengue virus (denv) and chikungunya virus (chikv) are attracted to specific host cues that are not generated by traditional light traps. for this reason multiple companies have designed traps to specifically target those species. recently the standard trap for denv and chikv vectors, the bg-sentinel (bgs) trap, has been remodeled to be more durable and better suited for use in harsh field conditions, common during military operations, and relabeled the bg-sentinel 2 ... | 2015 | 26375905 |
| [lessons learned in the control of aedes aegypti to address dengue and the emergency of chikungunya in iquitos, peru]. | dengue has affected iquitos since 1990 causing outbreaks of major impact on public health and for this reason great efforts have been made for its temporal control. currently, with the expansion of the chikungunya virus in the americas and the threat of the emergence of the virus in iquitos, we reflect on lessons learned by way of the activities undertaken in the area of vector control; epidemiological surveillance, diagnosis and clinical management during periods of outbreaks of dengue, in a wa ... | 2015 | 26102121 |
| susceptibility of aedes albopictus and aedes aegypti to three imported chikungunya virus strains, including the e1/226v variant in taiwan. | an e1/226v variant chikungunya virus (chikv) efficiently transmitted by aedes albopictus to humans poses a significant threat to public health for those areas with the presence of aedes albopictus, including taiwan. | 2015 | 25715998 |
| the consequences of co-infections for parasite transmission in the mosquito aedes aegypti. | co-infections may modify parasite transmission opportunities directly as a consequence of interactions in the within-host environment, but also indirectly through changes in host life history. furthermore, host and parasite traits are sensitive to the abiotic environment with variable consequences for parasite transmission in co-infections. we investigate how co-infection of the mosquito aedes aegypti with two microsporidian parasites (vavraia culicis and edhazardia aedis) at two levels of larva ... | 2015 | 25311642 |
| effects of artesunate treatment on plasmodium gallinaceum transmission in the vectors aedes aegypti and culex quinquefasciatus. | in the absence of vaccines, chemotherapy is an effective and economical way for controlling malaria. development of anti-malarial drugs that target pathogenic blood stage parasites and gametocytes is preferable for the treatment as it can alleviate the host's morbidity and mortality and block transmission of the plasmodium parasite. recently, our laboratory has developed an in vivo transmission blocking assay that involves administration of 7 consecutive daily doses of a test compound into domes ... | 2015 | 25466617 |
| di-rhamnolipid is a mosquito pupicidal metabolite from pseudomonas fluorescens (vcrc b426). | pseudomonas fluorescens migula (vcrc b426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely culex quinquefasciatus, anopheles stephensi and aedes aegypti. the mosquito pupicidal metabolite from p. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, fplc, hplc and tlc. the purified metabolite was characterized by nmr, ft-ir, lc-ms and maldi-tof. the ft-ir, (1)h and ( ... | 2015 | 25912083 |
| inter-epidemic abundance and distribution of potential mosquito vectors for rift valley fever virus in ngorongoro district, tanzania. | background rift valley fever (rvf) is a mosquito-borne viral zoonosis that primarily affects ruminants but also has the capacity to infect humans. objective to determine the abundance and distribution of mosquito vectors in relation to their potential role in the virus transmission and maintenance in disease epidemic areas of ngorongoro district in northern tanzania. methods a cross-sectional entomological investigation was carried out before the suspected rvf outbreak in october 2012. mosquitoe ... | 2015 | 28156815 |
| interaction of lysinibacillus sphaericus binary toxin with mosquito larval gut cells: binding and internalization. | the binary toxin produced by lysinibacillus sphaericus is composed of bina and binb subunits. together, but not separately, the two subunits are highly toxic to culex quinquefasciatus larvae, but show no toxicity to aedes aegypti. the molecular mechanism underlying intoxication has not been clearly elucidated. the present study compares the binding and the internalization of binary toxin into the midgut epithelial cells of susceptible c. quinquefasciatus mosquito larvae with those of bin-refract ... | 2015 | 26408968 |
| functional implications of the peptidoglycan recognition proteins in the immunity of the yellow fever mosquito, aedes aegypti. | peptidoglycan recognition proteins (pgrps) play essential roles in the immune systems of insects and higher animals against certain pathogens, including bacteria. in insects, most studies on the functions of pgrps have been performed in drosophila, with only limited studies in mosquitoes, which are important disease vectors. in the present study, we analysed the pgrp sequences of the yellow fever mosquito, aedes aegypti, acquired from two genome databases, and identified a total of seven pgrp ge ... | 2015 | 25588548 |
| molecular characterization of an apolipophorin-iii gene from the chinese oak silkworm, antheraea pernyi (lepidoptera: saturniidae). | apolipophorin-iii (apolp-iii) acts in lipid transport, lipoprotein metabolism, and innate immunity in insects. in this study, an apolp-iii gene of antheraea pernyi pupae (ap-apolp-iii) was isolated and characterized. the full-length cdna of ap-apolp-iii is 687 bp, including a 5'-untranslated region (utr) of 40 bp, 3'-utr of 86 bp and an open reading frame of 561 bp encoding a polypeptide of 186 amino acids that contains an apolipophorin-iii precursor domain (pf07464). the deduced ap-apolp-iii pr ... | 2015 | 25348706 |
| effects of proteinase inhibitor from adenanthera pavonina seeds on short- and long term larval development of aedes aegypti. | currently, one of the major global public health concerns is related to the transmission of dengue/yellow fever virus by the vector aedes aegypti. the most abundant digestive enzymes in ae. aegypti midgut larvae are trypsin and chymotrypsin. since protease inhibitors have the capacity to bind to and inhibit the action of insect digestive proteinases, we investigated the short- and long-term effects of adenanthera pavonina seed proteinase inhibitor (apti) on ae. aegypti larvae, as well as a possi ... | 2015 | 25796215 |
| [management of the yellow fever epidemic in 2010 in séguéla (côte d'ivoire): value of multidisciplinary investigation]. | in august 2010, five positive cases of yellow fever were reported in the region of séguéla, in the northwest of côte d'ivoire, affected by an armed conflict since 2002. in order to evaluate the extent of yellow fever virus circulation and the risk for local people, a multidisciplinary investigation was carried out by the côte-d'ivoire ministry of health and public hygiene. | 2015 | 25629680 |
| behavioral response of aedes aegypti (diptera: culicidae) larvae to synthetic and natural attractants and repellents. | aedes aegypti (l.) (diptera: culicidae) is the key vector of three important arboviral diseases: dengue, yellow fever, and chikungunya. immature stages of this species inhabit human-made containers placed in residential landscapes. in this study, we evaluated a few compounds in a sensitive behavioral assay with ae. aegypti larvae. the orientation of larvae to different compounds was surveyed using a performance index (pi). the pi represents the response to each odorant, where a value of +1 is in ... | 2015 | 26352935 |
| efficient transposition of the youngest miniature inverted repeat transposable element family of yellow fever mosquito in yeast. | miniature inverted repeat transposable elements (mites) are often the most numerous dna transposons in plant and animal genomes. the dramatic amplification of mite families during evolution is puzzling, because the transposase sources for the vast majority of mite families are unknown. the yellow fever mosquito genome contains > 220-mb mite sequences; however, transposition activity has not been demonstrated for any of the mite families. the gnome elements are the youngest mite family in this ge ... | 2015 | 25754725 |
| chemical composition and biological activity of essential oils of dracocephalum heterophyllum and hyssopus officinalis from western himalaya. | the essential oils of two representatives of the lamiaceae, dracocephalum heterophyllum benth. and hyssopus officinalis l., are described for their antifungal, antibacterial, larvicidal and inect biting deterrent activities. additionally, the chemical compositions of the essential oils, analyzed by simultaneous gc-ms and gc-fid, and odor descriptions are given. the main components of h. officinalis oil were pinocarvone, cis-pinocamphone, and β-pinene. citronellol was found as the main compound o ... | 2015 | 25920235 |
| mosquito larvicidal and pupaecidal potential of prodigiosin from serratia marcescens and understanding its mechanism of action. | mosquitoes spread lethal diseases like malaria and dengue fever to humans. considering mosquito vector control as one of the best alternatives to reduce new infections, here we have analyzed the effect of purified pigment prodigiosin extracted from serratia marcescens (nmcc 75) against larval and pupal stages of aedes aegypti and anopheles stephensi mosquitoes. mosquito larvicidal activities of purified prodigiosin revealed lc50 values of 14 ± 1.2, 15.6 ± 1.48, 18 ± 1.3, 21 ± 0.87 µg/ml against ... | 2015 | 26267052 |
| virulency of novel nanolarvicide from trichoderma atroviride against aedes aegypti (linn.): a clsm analysis. | aedes aegypti is the vector for transmitting dengue, chikungunya, and yellow fever. these diseases' transmission has increased predominantly in urban and semi-urban areas as a major public health concern. in present investigation, trichoderma atroviride culture filtrates were used for the synthesis of silver nanoparticle. moreover, t. atroviride is a free-living and rapidly growing fungi common in soil and root ecosystem. this fungi is an exceptionally good model for biocontrol and more signific ... | 2015 | 25907629 |
| efficacy of tolypocladium cylindrosporum against aedes aegypti eggs, larvae and adults. | the aim of this study was to test the activity of seven tolypocladium cylindrosporum isolates against different developmental stages of aedes aegypti. | 2015 | 26332164 |
| [detection of flavivirus in mosquitoes (diptera: culicidae) from easter island-chile]. | flaviviruses are arthropod-borne viruses, mainly by mosquitoes of the genera aedes and culex (culicidae) that are detected in tropical and subtropical areas. main flaviviruses of public health importance are: dengue, west nile virus, yellow fever, among others. in continental chile, flaviviruses has not been detected. however, there are indigenous cases of dengue detected in easter island since 2002, as the presence of its vector aedes aegypti. the aim of this study was: to determine diversity o ... | 2015 | 25860055 |
| ovicidal and adulticidal potential of leaf and seed extract of albizia lebbeck (l.) benth. (family: fabaceae) against culex quinquefasciatus, aedes aegypti, and anopheles stephensi (diptera: culicidae). | several diseases are associated to the mosquito-human interaction. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. in the present study, hexane, benzene, chloroform, ethyl acetate, and methanol extracts of leaf and seed of albizia lebbeck were assayed for their toxicity agai ... | 2015 | 25681143 |
| laboratory evaluation of indian medicinal plants as repellents against malaria, dengue, and filariasis vector mosquitoes. | mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world ... | 2015 | 25399815 |
| [zika virus: another emerging arbovirus in venezuela?]. | current health conditions of venezuela, together with the tropical proliferation of different types of aedes (aegypti and albopictus), suggest the arrival to this country of zika virus (zikv). the pan american health organization urges latin american countries to take measures to prevent the zikv entry and advises that these measures must be designed to detect the introduction of zikv in an area, track its spread and actively monitor the disease. in venezuela and other latin-american countries, ... | 2015 | 26710538 |
| screening for mosquito larvicidal activity of thai mushroom extracts with special reference to steccherinum sp against aedes aegypti (l.) (diptera: culicidae). | for over 50 years, biological control of mosquito larvae has depended mainly on plant extracts, fish, bacteria, protozoa, filamentous fungi, viruses or nematodes. in this study, we screened 143 mushroom samples from 44 confirmed species in thailand for their mosquito larvicidal activity. one g% (w/v) aqueous extracts of dried powdered mushroom samples were tested against 3rd stage aedes aegypti larvae. four mushroom species, namely, thaeogyroporus porentosus, xylaria nigripes, chlorophyllum sp a ... | 2015 | 26867377 |
| absence of aedes aegypti (l.) on an ecological island: competitive exclusion? | ovitrap surveillance was conducted in 2012 and 2006 in malay and aboriginal villages on carey island. in each village, standard ovitraps were placed indoors and outdoors at randomly selected houses/locations. all l3 larvae recovered were identified up to species level. results demonstrated that only larvae of aedes albopictus were found in all the positive ovitraps placed indoors and outdoors. in 2012, a high ovitrap index (oi) of 66.7% indoor and 84.0% outdoor in the malay village; and 62.5% in ... | 2015 | 26863856 |
| forced egg retention induced by diethyl-phenylacetamide diminishes the fecundity and longevity of dengue vectors. | oviposition is an important event in the life cycle of mosquito which is a decisive factor for the fate of its progeny. if oviposition is prevented, the life cycle of mosquito is disrupted and the population growth can be reduced. diethyl-phenylacetamide (depa) is an effective topical repellent against various haematophagous insects. the study reports the egg retention effect and subsequent lethal effect of depa on the gravid females of aedes aegypti and ae. albopictus mosquitoes. | 2015 | 26714511 |
| is there a risk of chikungunya transmission in canada? | an outbreak of chikungunya virus infection is currently underway in the caribbean, central america and south america, and autochthonous (local or indigenous) transmission has occurred in the southeastern united states. the mosquito species known to transmit chikungunya-aedes aegypti and aedes albopictus-are not known to reside in canada at this time. but how comfortable can we be that this situation will continue? here we explore four key conditions that must be met for transmission of chikungun ... | 2015 | 29769915 |
| annotation of the asian citrus psyllid genome reveals a reduced innate immune system. | citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as huanglongbing. the citrus greening bacteria, candidatus liberibacter asiaticus (clas), is a persistent propagative pathogen transmitted by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). hemipterans characterized to date lack a number of insect immune genes, including those associated with the imd pathway targeting gram-negative bacteria. the d. citri draft ... | 2016 | 27965582 |
| disruption of phenylalanine hydroxylase reduces adult lifespan and fecundity, and impairs embryonic development in parthenogenetic pea aphids. | phenylalanine hydroxylase (pah) is a key tyrosine-biosynthetic enzyme involved in neurological and melanin-associated physiological processes. despite extensive investigations in holometabolous insects, a pah contribution to insect embryonic development has never been demonstrated. here, we have characterized, for the first time, the pah gene in a hemimetabolous insect, the aphid acyrthosiphon pisum. phylogenetic and sequence analyses confirmed that appah is closely related to metazoan pah, exhi ... | 2016 | 27694983 |
| convergent evolution of trna gene targeting preferences in compact genomes. | in gene-dense genomes, mobile elements are confronted with highly selective pressure to amplify without causing excessive damage to the host. the targeting of trna genes as potentially safe integration sites has been developed by retrotransposons in various organisms such as the social amoeba dictyostelium discoideum and the yeast saccharomyces cerevisiae. in d. discoideum, trna gene-targeting retrotransposons have expanded to approximately 3 % of the genome. recently obtained genome sequences o ... | 2016 | 27583033 |
| small rna regulators of plant-hemipteran interactions: micromanagers with versatile roles. | non-coding small rnas (srnas) in plants have important roles in regulating biological processes, including development, reproduction, and stress responses. recent research indicates significant roles for srna-mediated gene silencing during plant-hemipteran interactions that involve all three of these biological processes. plant responses to hemipteran feeding are determined by changes in the host transcriptome that appear to be fine-tuned by srnas. the role of srna in plant defense responses is ... | 2016 | 27625654 |
| novel insights into insect-microbe interactions-role of epigenomics and small rnas. | it has become increasingly clear that microbes form close associations with the vast majority of animal species, especially insects. in fact, an array of diverse microbes is known to form shared metabolic pathways with their insect hosts. a growing area of research in insect-microbe interactions, notably for hemipteran insects and their mutualistic symbionts, is to elucidate the regulation of this inter-domain metabolism. this review examines two new emerging mechanisms of gene regulation and th ... | 2016 | 27540386 |
| heat sensitivity of wmel wolbachia during aedes aegypti development. | the wmel strain of wolbachia bacteria is known to prevent dengue and zika virus transmission in the mosquito vector aedes aegypti. accordingly, the release of wmel-infected a. aegypti in endemic regions has been recommended by the world health organization as a potential strategy for controlling dengue and zika outbreaks. however, the utility of this approach could be limited if high temperatures in the aquatic habitats where a. aegypti develop are detrimental to wolbachia. we exposed wmel-infec ... | 2016 | 27459519 |
| de novo transcriptome assembly of the grapevine phylloxera allows identification of genes differentially expressed between leaf- and root-feeding forms. | grapevine phylloxera, an insect related to true aphids, is a major historic pest of viticulture only controlled through the selection of resistant rootstocks or through quarantine regulations where grapevine is cultivated own-rooted. transcriptomic data could help understand the bases of its original life-traits, including a striking case of polyphenism, with forms feeding on roots and forms feeding in leaf-galls. comparisons with true aphids (for which complete genomes have been sequenced) shou ... | 2016 | 26968158 |
| detection of dengue, west nile virus, rickettsiosis and leptospirosis by a new real-time pcr strategy. | dengue virus (denv) infection causes sudden fever along with several nonspecific signs and symptoms and in severe cases, death. denv is transmitted to people by aedes aegypti and ae. albopictus mosquitoes, whose populations increase during rainy season. west nile virus (wnv), rickettsia spp. and leptospira spp. are fever-causing pathogens that share many of the initial symptoms of denv infection and also thrive in the rainy season. outbreaks in some regions may be due to any of these pathogens t ... | 2016 | 27350908 |
| zika virus: an update on epidemiology, pathology, molecular biology, and animal model. | zika virus (zikv) was first described in 1947, and became a health emergency problem in 2016 when its association with fetal microcephaly cases was confirmed by centers for disease control and prevention (cdc) in the united states. to date, zikv infection has been documented in 66 countries. zikv is recognized as a neurotropic virus and numerous diseases manifested in multiple neurological disorders have been described, mainly in countries that have been exposed to zikv after the 2007 outbreak i ... | 2016 | 27124623 |
| magnitude of neglected tropical diseases in indonesia at postmillennium development goals era. | the world will enter the postmillennium development goals 2015 era. the achievements of the millennium development goals (mdgs) as a global development target need to be evaluated. a sustainable new reasonable target is important for neglected tropical diseases (ntd) elimination in indonesia. this review describes the ntd situation in indonesia and highlights problems beneath the ntd transmission. multidisciplinary approach is a promising strategy to help the marginalized people. | 2016 | 27190525 |
| the untapped cell biology of neglected tropical diseases. | the world health organization lists a constellation of 17 tropical diseases that afflict approximately one in six individuals on the planet and, until recently, few resources have been devoted to the treatment and eradication of those diseases. they are often referred to as the diseases of the "bottom billion," because they are most prevalent among the poorest individuals in impoverished tropical nations. however, the few studies that have been performed reveal an extraordinary world of molecula ... | 2016 | 26915691 |
| structure and functional diversity of gcn5-related n-acetyltransferases (gnat). | general control non-repressible 5 (gcn5)-related n-acetyltransferases (gnat) catalyze the transfer of an acyl moiety from acyl coenzyme a (acyl-coa) to a diverse group of substrates and are widely distributed in all domains of life. this review of the currently available data acquired on gnat enzymes by a combination of structural, mutagenesis and kinetic methods summarizes the key similarities and differences between several distinctly different families within the gnat superfamily, with an emp ... | 2016 | 27367672 |
| overview of zika virus (zikv) infection in regards to the brazilian epidemic. | zika virus (zikv), a mosquito-borne flavivirus, belongs to the flaviviridae family, genus flavivirus. zikv was initially isolated in 1947 from a sentinel monkey in the zika forest, uganda. little clinical importance was attributed to zikv, once only few symptomatic cases were reported in some african and southeast asiatic countries. this situation changed in 2007, when a large outbreak was registered on the yap island, micronesia, caused by the asian zikv lineage. between 2013 and 2014, zikv spr ... | 2016 | 27143174 |
| zika: the origin and spread of a mosquito-borne virus. | to describe the temporal and geographical distribution of zika virus infection and associated neurological disorders, from 1947 to 1 february 2016, when zika became a public health emergency of international concern (pheic). | 2016 | 27708473 |
| persistent infection by wolbachia walbb has no effect on composition of the gut microbiota in adult female anopheles stephensi. | the bacteria in the midgut of anopheles stephensi adult females from laboratory colonies were studied by sequencing the v4 region of 16s rrna genes, with respect to three experimental factors: stable or cured wolbachia infection; sugar or blood diet; and age. proteobacteria and bacteroidetes dominated the community [>90% of operational taxonomic units (otus)]; most taxa were in the classes flavobacteriia, gammaproteobacteria, and alphaproteobacteria, and were assigned to elizabethkingia (46.9%), ... | 2016 | 27708633 |
| functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts. | as both larvae and adults, mosquitoes encounter a barrage of immune insults, ranging from microbe-rich communities in larval habitats to ingested blood-borne pathogens in adult blood meals. given that mosquito adults have evolved an efficient means of eliminating infections in their hemocoel (body cavity) via the coordinated action of their immune and circulatory systems, the goal of the present study was to determine whether such functional integration is also present in larvae. | 2016 | 27643786 |
| mosquito c-type lectins maintain gut microbiome homeostasis. | the long-term evolutionary interaction between the host immune system and symbiotic bacteria determines their cooperative rather than antagonistic relationship. it is known that commensal bacteria have evolved a number of mechanisms to manipulate the mammalian host immune system and maintain homeostasis. however, the strategies employed by the microbiome to overcome host immune responses in invertebrates still remain to be understood. here, we report that the gut microbiome in mosquitoes utilize ... | 2016 | 27170846 |
| open-air biowarfare testing and the evolution of values. | the united states and the united kingdom ended outdoor biological warfare testing in populated areas nearly half a century ago. yet, the conduct, health effects, and propriety of those tests remain controversial. the varied views reflect the limits of currently available test information and evolving societal values on research involving human subjects. western political culture has changed since the early days of the american and british testing programs. people have become less reluctant to qu ... | 2016 | 27564984 |
| symbiosis with francisella tularensis provides resistance to pathogens in the silkworm. | francisella tularensis, the causative agent of tularemia, is a highly virulent facultative intracellular pathogen found in a wide range of animals, including arthropods, and environments. this bacterium has been known for over 100 years, but the lifestyle of f. tularensis in natural reservoirs remains largely unknown. thus, we established a novel natural host model for f. tularensis using the silkworm (bombyx mori), which is an insect model for infection by pathogens. f. tularensis established a ... | 2016 | 27507264 |
| advances and limitations of disease biogeography using ecological niche modeling. | mapping disease transmission risk is crucial in public and animal health for evidence based decision-making. ecology and epidemiology are highly related disciplines that may contribute to improvements in mapping disease, which can be used to answer health related questions. ecological niche modeling is increasingly used for understanding the biogeography of diseases in plants, animals, and humans. however, epidemiological applications of niche modeling approaches for disease mapping can fail to ... | 2016 | 27547199 |
| diversity, evolution and medical applications of insect antimicrobial peptides. | antimicrobial peptides (amps) are short proteins with antimicrobial activity. a large portion of known amps originate from insects, and the number and diversity of these molecules in different species varies considerably. insect amps represent a potential source of alternative antibiotics to address the limitation of current antibiotics, which has been caused by the emergence and spread of multidrug-resistant pathogens. to get more insight into amps, we investigated the diversity and evolution o ... | 2016 | 27160593 |
| histopathology of aedes aegypti (diptera: culicidae) larvae parasitized by reesimermis nielseni (nematoda: mermithidae). | 2016 | 4761359 | |
| esterase polymorphism in aedes aegypti: the genetics and k m values of electrophoretically heterogeneous forms. | 2016 | 5038250 | |
| frequency of blood feeding in the mosquito aedes aegypti. | 2016 | 4937213 | |
| systemically and cutaneously distributed ectoparasiticides: a review of the efficacy against ticks and fleas on dogs. | acaricidal (tick) and insecticidal (flea) efficacy of systemically and cutaneously distributed ectoparasiticide products for dogs are compared based on permethrin and fluralaner as representative molecules. results of efficacy studies against fleas and ticks are reviewed that show generally good to excellent results. both externally and systemically distributed treatments have benefits and weaknesses in potentially preventing pathogen transmission by these arthropod vectors.four general properti ... | 2016 | 27502490 |
| vaccines and immunization strategies for dengue prevention. | dengue is currently the most significant arboviral disease afflicting tropical and sub-tropical countries worldwide. dengue vaccines, such as the multivalent attenuated, chimeric, dna and inactivated vaccines, have been developed to prevent dengue infection in humans, and they function predominantly by stimulating immune responses against the dengue virus (denv) envelope (e) and nonstructural-1 proteins (ns1). of these vaccines, a live attenuated chimeric tetravalent denv vaccine developed by sa ... | 2016 | 27436365 |
| disease vectors in the era of next generation sequencing. | almost 20 % of all infectious human diseases are vector borne and, together, are responsible for over one million deaths per annum. over the past decade, the decreasing costs of massively parallel sequencing technologies have facilitated the agnostic interrogation of insect vector genomes, giving medical entomologists access to an ever-expanding volume of high-quality genomic and transcriptomic data. in this review, we highlight how genomics resources have provided new insights into the physiolo ... | 2016 | 27154554 |
| genomic insights into the ixodes scapularis tick vector of lyme disease. | ticks transmit more pathogens to humans and animals than any other arthropod. we describe the 2.1 gbp nuclear genome of the tick, ixodes scapularis (say), which vectors pathogens that cause lyme disease, human granulocytic anaplasmosis, babesiosis and other diseases. the large genome reflects accumulation of repetitive dna, new lineages of retro-transposons, and gene architecture patterns resembling ancient metazoans rather than pancrustaceans. annotation of scaffolds representing ∼57% of the ge ... | 2016 | 26856261 |
| identification of abcc2 as a binding protein of cry1ac on brush border membrane vesicles from helicoverpa armigera by an improved pull-down assay. | cry1ac toxin-binding proteins from helicoverpa armigera brush border membrane vesicles were identified by an improved pull-down method that involves coupling cry1ac to cnbr agarose combined with liquid chromatography-tandem mass spectrometry (lc-ms/ms). according to the lc-ms/ms results, cry1ac toxin could bind to six classes of aminopeptidase-n, alkaline phosphatase, cadherin-like protein, atp-binding cassette transporter subfamily c protein (abcc2), actin, atpase, polycalin, and some other pro ... | 2016 | 27037552 |
| unique features of a global human ectoparasite identified through sequencing of the bed bug genome. | the bed bug, cimex lectularius, has re-established itself as a ubiquitous human ectoparasite throughout much of the world during the past two decades. this global resurgence is likely linked to increased international travel and commerce in addition to widespread insecticide resistance. analyses of the c. lectularius sequenced genome (650 mb) and 14,220 predicted protein-coding genes provide a comprehensive representation of genes that are linked to traumatic insemination, a reduced chemosensory ... | 2016 | 26836814 |
| mosquito-transmitted viruses - the great brazilian challenge. | arboviruses pose a serious threat to public health worldwide, overloading the healthcare system and causing economic losses. these viruses form a very diverse group, and in brazil, arboviruses belonging to the families flaviviridae and togaviridae are predominant. unfortunately, the number of arboviruses increases in proportion with factors such as deforestation, poor sanitation, climate changes, and introduction of new viruses like chikungunya virus and zika virus. in brazil, dengue is endemic, ... | 2016 | 27818091 |
| the viral capping enzyme nsp1: a novel target for the inhibition of chikungunya virus infection. | the chikungunya virus (chikv) has become a substantial global health threat due to its massive re-emergence, the considerable disease burden and the lack of vaccines or therapeutics. we discovered a novel class of small molecules ([1,2,3]triazolo[4,5-d]pyrimidin-7(6h)-ones) with potent in vitro activity against chikv isolates from different geographical regions. drug-resistant variants were selected and these carried a p34s substitution in non-structural protein 1 (nsp1), the main enzyme involve ... | 2016 | 27545976 |
| mosquito communities and disease risk influenced by land use change and seasonality in the australian tropics. | anthropogenic land use changes have contributed considerably to the rise of emerging and re-emerging mosquito-borne diseases. these diseases appear to be increasing as a result of the novel juxtapositions of habitats and species that can result in new interchanges of vectors, diseases and hosts. we studied whether the mosquito community structure varied between habitats and seasons and whether known disease vectors displayed habitat preferences in tropical australia. | 2016 | 27388293 |
| alphavirus infection: host cell shut-off and inhibition of antiviral responses. | alphaviruses cause debilitating disease in humans and animals and are transmitted by blood-feeding arthropods, typically mosquitoes. with a traditional focus on two models, sindbis virus and semliki forest virus, alphavirus research has significantly intensified in the last decade partly due to the re-emergence and dramatic expansion of chikungunya virus in asia, europe, and the americas. as a consequence, alphavirus-host interactions are now understood in much more molecular detail, and importa ... | 2016 | 27294951 |
| design and testing of novel lethal ovitrap to reduce populations of aedes mosquitoes: community-based participatory research between industry, academia and communities in peru and thailand. | dengue virus (and chikungunya and zika viruses) is transmitted by aedes aegypti and aedes albopictus mosquitoes and causes considerable human morbidity and mortality. as there is currently no vaccine or chemoprophylaxis to protect people from dengue virus infection, vector control is the only viable option for disease prevention. the purpose of this paper is to illustrate the design and placement process for an attractive lethal ovitrap to reduce vector populations and to describe lessons learne ... | 2016 | 27532497 |
| wolbachia biocontrol strategies for arboviral diseases and the potential influence of resident wolbachia strains in mosquitoes. | arboviruses transmitted by mosquitoes are a major cause of human disease worldwide. the absence of vaccines and effective vector control strategies has resulted in the need for novel mosquito control strategies. the endosymbiotic bacterium wolbachia has been proposed to form the basis for an effective mosquito biocontrol strategy. resident strains of wolbachia inhibit viral replication in drosophila fruit flies and induce a reproductive phenotype known as cytoplasmic incompatibility that allows ... | 2016 | 26925368 |
| arbovirosis and potential transmission blocking vaccines. | infectious diseases caused by arboviruses (viruses transmitted by arthropods) are undergoing unprecedented epidemic activity and geographic expansion. with the recent introduction of west nile virus (1999), chikungunya virus (2013) and zika virus (2015) to the americas, stopping or even preventing the expansion of viruses into susceptible populations is an increasing concern. with a few exceptions, available vaccines protecting against arboviral infections are nonexistent and current disease pre ... | 2016 | 27664127 |
| characterization of drosophila cmp-sialic acid synthetase activity reveals unusual enzymatic properties. | cmp-sialic acid synthetase (csas) is a key enzyme of the sialylation pathway. csas produces the activated sugar donor, cmp-sialic acid, which serves as a substrate for sialyltransferases to modify glycan termini with sialic acid. unlike other animal csass that normally localize in the nucleus, drosophila melanogaster csas (dmcsas) localizes in the cell secretory compartment, predominantly in the golgi, which suggests that this enzyme has properties distinct from those of its vertebrate counterpa ... | 2016 | 27114558 |
| use of a probabilistic motif search to identify histidine phosphotransfer domain-containing proteins. | the wealth of newly obtained proteomic information affords researchers the possibility of searching for proteins of a given structure or function. here we describe a general method for the detection of a protein domain of interest in any species for which a complete proteome exists. in particular, we apply this approach to identify histidine phosphotransfer (hpt) domain-containing proteins across a range of eukaryotic species. from the sequences of known hpt domains, we created an amino acid occ ... | 2016 | 26751210 |
| the sandfly lutzomyia longipalpis ll5 embryonic cell line has active toll and imd pathways and shows immune responses to bacteria, yeast and leishmania. | lutzomyia longipalpis is the main vector of visceral leishmaniasis in latin america. sandfly immune responses are poorly understood. in previous work we showed that these vector insects respond to bacterial infections by modulating a defensin gene expression and activate the imd pathway in response to leishmania infection. aspects of innate immune pathways in insects (including mosquito vectors of human diseases) have been revealed by studying insect cell lines, and we have previously demonstrat ... | 2016 | 27098567 |
| emerging role of lipid droplets in aedes aegypti immune response against bacteria and dengue virus. | in mammals, lipid droplets (lds) are ubiquitous organelles that modulate immune and inflammatory responses through the production of lipid mediators. in insects, it is unknown whether lds play any role during the development of immune responses. we show that aedes aegypti aag2 cells - an immune responsive cell lineage - accumulates lds when challenged with enterobacter cloacae, sindbis, and dengue viruses. microarray analysis of aag2 challenged with e.cloacae or infected with dengue virus reveal ... | 2016 | 26887863 |
| mass spectrometry analysis and transcriptome sequencing reveal glowing squid crystal proteins are in the same superfamily as firefly luciferase. | the japanese firefly squid hotaru-ika (watasenia scintillans) produces intense blue light from photophores at the tips of two arms. these photophores are densely packed with protein microcrystals that catalyse the bioluminescent reaction using atp and the substrate coelenterazine disulfate. the squid is the only organism known to produce light using protein crystals. we extracted microcrystals from arm tip photophores and identified the constituent proteins using mass spectrometry and transcript ... | 2016 | 27279452 |
| tetrapyrroles as endogenous tspo ligands in eukaryotes and prokaryotes: comparisons with synthetic ligands. | the 18 kda translocator protein (tspo) is highly 0conserved in eukaryotes and prokaryotes. since its discovery in 1977, numerous studies established the tspo's importance for life essential functions. for these studies, synthetic tspo ligands typically are applied. tetrapyrroles present endogenous ligands for the tspo. tetrapyrroles are also evolutionarily conserved and regulate multiple functions. tspo and tetrapyrroles regulate each other. in animals tspo-tetrapyrrole interactions range from e ... | 2016 | 27271616 |
| novel nad+-farnesal dehydrogenase from polygonum minus leaves. purification and characterization of enzyme in juvenile hormone iii biosynthetic pathway in plant. | juvenile hormone iii is of great concern due to negative effects on major developmental and reproductive maturation in insect pests. thus, the elucidation of enzymes involved jh iii biosynthetic pathway has become increasing important in recent years. one of the enzymes in the jh iii biosynthetic pathway that remains to be isolated and characterized is farnesal dehydrogenase, an enzyme responsible to catalyze the oxidation of farnesal into farnesoic acid. a novel nad+-farnesal dehydrogenase of p ... | 2016 | 27560927 |
| functional genomic analyses of enterobacter, anopheles and plasmodium reciprocal interactions that impact vector competence. | malaria exerts a tremendous socioeconomic impact worldwide despite current control efforts, and novel disease transmission-blocking strategies are urgently needed. the enterobacter bacterium esp_z, which is naturally harboured in the mosquito midgut, can inhibit the development of plasmodium parasites prior to their invasion of the midgut epithelium through a mechanism that involves oxidative stress. here, a multifaceted approach is used to study the tripartite interactions between the mosquito, ... | 2016 | 27549662 |
| depletion of alveolar macrophages does not prevent hantavirus disease pathogenesis in golden syrian hamsters. | andes virus (andv) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (hps). the mechanism for the massive vascular leakage associated with hps is poorly understood; however, dysregulation of components of the immune response is often suggested as a possible cause. alveolar macrophages are found in the alveoli of the lung and represent the first line of defense to many airborne pathogens. to determine whether alveolar macrophages play a role in hps ... | 2016 | 27099308 |
| analysis of protein composition and bioactivity of neoponera villosa venom (hymenoptera: formicidae). | ants cause a series of accidents involving humans. such accidents generate different reactions in the body, ranging from a mild irritation at the bite site to anaphylactic shock, and these reactions depend on the mechanism of action of the venom. the study of animal venom is a science known as venomics. through venomics, the composition of the venom of several ant species has already been characterized and their biological activities described. thus, the aim of this study was to evaluate the pro ... | 2016 | 27110765 |
| tiger mosquito - generated devastation in the biosphere!!! | 2016 | 27041891 | |
| how to predict molecular interactions between species? | organisms constantly interact with other species through physical contact which leads to changes on the molecular level, for example the transcriptome. these changes can be monitored for all genes, with the help of high-throughput experiments such as rna-seq or microarrays. the adaptation of the gene expression to environmental changes within cells is mediated through complex gene regulatory networks. often, our knowledge of these networks is incomplete. network inference predicts gene regulator ... | 2016 | 27065992 |
| transfer of dna from bacteria to eukaryotes. | historically, the members of the agrobacterium genus have been considered the only bacterial species naturally able to transfer and integrate dna into the genomes of their eukaryotic hosts. yet, increasing evidence suggests that this ability to genetically transform eukaryotic host cells might be more widespread in the bacterial world. indeed, analyses of accumulating genomic data reveal cases of horizontal gene transfer from bacteria to eukaryotes and suggest that it represents a significant fo ... | 2016 | 27406565 |
| horizontal transfer of β-carbonic anhydrase genes from prokaryotes to protozoans, insects, and nematodes. | horizontal gene transfer (hgt) is a movement of genetic information occurring outside of normal mating activities. it is especially common between prokaryotic endosymbionts and their protozoan, insect, and nematode hosts. although beta carbonic anhydrase (β-ca) plays a crucial role in metabolic functions of many living organisms, the origin of β-ca genes in eukaryotic species remains unclear. | 2016 | 26983858 |