Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| nuclear magnetic resonance probes for investigations of the virus-cell interactions. | measuring the nmr relaxation parameters of selectively excited protons within molecules bound to the cell membrane was shown to provide a profitable way of looking at the virus-cell interaction. such approach made it possible to delineate the virus adsorption to human red blood cells by using the nmr features of the cytoskeleton inhibitor colchicine. | 1985 | 4094564 |
| myocardial lesions by coxsackie virus b3 and cytomegalovirus infection in infants. | immunofluorescent and electron-microscopic studies were performed to determine the distribution of viral antigens and particles and to clarify the relationship to myocardial lesions in two autopsy cases with generalized infection of coxsackie virus b3 (cvb3) or cytomegalovirus (cmv). case 1 was a full-term newborn female infant, without any congenital anomalies, who died of cardiac failure 10 days after birth. cvb3 was isolated from the blood before death. necrosis of the muscle fibers was obser ... | 1985 | 3038831 |
| cell-mediated immunity in coxsackie b3 virus myocarditis in mice--in situ characterization by monoclonal antibody of mononuclear cell infiltrates. | this light- and electron-microscopic study using monoclonal antibody and anti-immunoglobulin antibodies in murine coxsackie b3 virus myocarditis provides an immunohistochemical demonstration of surface antigens of lymphocytes. on the 7th and 9th days after inoculation, many necrotic cardiocytes were surrounded by numerous cellular infiltrates, in which macrophages and t lymphocytes predominated, whereas immunoglobulin-bearing b lymphocytes represented a minority. immuno-electron microscopy showe ... | 1985 | 3038832 |
| coxsackie b, mumps, rubella, and cytomegalovirus specific igm responses in patients with juvenile-onset insulin-dependent diabetes mellitus in britain, austria, and australia. | patients from england, austria, and australia with recently diagnosed juvenile-onset insulin-dependent diabetes (type 1) mellitus (iddm) and matched controls were tested for specific igm responses to coxsackie b1-5 viruses. 37 of 122 (30%) patients aged less than 15, but only 15 of 204 (6%) controls, were positive (p less than 0.005). differences in coxsackie b virus specific igm responses between patients and controls were statistically significant for patients in england and austria (p less th ... | 1985 | 2861361 |
| insulin, glucagon and somatostatin content of the non-obese diabetic (nod) mouse pancreas and plasma virus antibodies to coxsackie b- and reoviruses. | the amounts of insulin, glucagon and somatostatin in the pancreas of nod mice were determined and the results were compared with those of normal icr-strain mice, and plasma antibodies to coxsackie b-3 and reovirus types 1, 2 and 3 were measured. in the pancreas of nod mice with fasting plasma glucose (fpg) less than 140 mg/100 ml, the insulin content of the male mice was similar to that of the normal controls, ranging to 3.55 +/- 0.31 u/g wet weight of pancreas, but it was already significantly ... | 1985 | 2862717 |
| coxsackie b virus and juvenile-onset insulin-dependent diabetes. | 1985 | 2863487 | |
| coxsackie-b-virus-specific igm responses, complement-fixing islet-cell antibodies, hla dr antigens, and c-peptide secretion in insulin-dependent diabetes mellitus. | to evaluate the role of coxsackie b viruses in the pathogenesis of insulin-dependent (juvenile-onset, type 1) diabetes mellitus (iddm), attempts were made to correlate virus-specific igm responses with hla genes, autoimmune responses, and c-peptide secretion. hla dr3, dr4, or both were present in 73 of 90 (81%) diabetic patients; 22 of 23 (96%) with coxsackie-b-virus-specific igm had at least one of these hla types, compared with 51 of 67 (76%) without virus-specific igm. there was no correlatio ... | 1985 | 2863632 |
| concentration of viruses from water by using cellulose filters modified by in situ precipitation of ferric and aluminum hydroxides. | untreated cellulose filters adsorbed only small amounts of poliovirus 1, echovirus 5, coxsackievirus b5, or bacteriophage ms2 that were added to tap water or to solutions of imidazole-glycine buffer at ph 5 to 7. modification of filters by in situ flocculation of ferric and aluminum hydroxides greatly increased the ability of the filters to adsorb viruses. viruses adsorbed to the modified filters could be recovered by treating the filters with 3% beef extract (ph 9.5). greater than 60% of the en ... | 1985 | 3004332 |
| influence of inoculum size, incubation temperature, and cell culture density on virus detection in environmental samples. | the influence of inoculum size and cell culture density on virus titer by cytopathic effect or plaque assay was studied using poliovirus type 1 and bgm (buffalo green monkey) cells as a model for this evaluation. with a plaque assay system, a linear relationship was observed for an inoculum size of up 1 ml/25 cm2; a marked decrease in the number of plaques was observed when over 1 ml of sample was inoculated on this surface area. cell culture density also affected virus titer; maximal titers wer ... | 1985 | 3004683 |
| a comparison of the susceptibility of three human gut tumour-derived differentiated epithelial cell lines, primary monkey kidney cells and human rhabdomyosarcoma cell line to 66-prototype strains of human enteroviruses. | the growth of prototype strains of 31 serotypes of echo, 3 polio, 6 coxsackie b, 24 coxsackie a and enterovirus serotypes 70 and 71 were tested in parallel in primary monkey kidney cells (pmk), rd cells and three gut tumour-derived differentiated epithelial cell lines (hrt-18 ht-29 in skco-1). all 31 serotypes of echo viruses grew in ht-29, 27 and skco-1, 5 in hrt-18, 29 in pmk and 29 in rd. there was good growth of poliovirus serotypes in all five cell types. coxsackie b viruses grew well in al ... | 1985 | 3009511 |
| coxsackie b-2 virus infection in rat beating heart cell culture. | beating rat heart cultures were prepared in vitro and infected with coxsackie b-2 virus. the cells were evaluated in the post-infected period for changes in cardiac enzymes, alterations in beating frequency and cytotoxicity as measured by chromium 51 (51cr) release. the cardiac enzymes, lactate dehydrogenase (ldh) and aspartate aminotransferase (ast) were measured in infected and uninfected controls over a period of 120 h. enzyme levels in the infected cells remained essentially the same for the ... | 1985 | 3009512 |
| use of the nitrocellulose-enzyme immunosorbent assay for rapid, sensitive and quantitative detection of human enteroviruses. | a modified enzyme immunosorbent assay (eia) employing nitrocellulose (nc) membrane as a high-capacity solid phase was successfully employed for the sensitive and rapid detection of human enteric viruses, poliovirus and coxsackievirus b-5. the sensitivity of the nc-eia ranged from 7 to 70 pg of viral antigen diluted in phosphate-buffered saline. when virus was added to crude supernatants of mollusc tissue homogenates prepared by the standard procedure for the recovery of viruses in molluscs, the ... | 1985 | 3009513 |
| detection by immunofluorescence of possible viral implications in "idiopathic" peripheral facial paralysis. | the presence of viral antigens was detected by the indirect immunofluorescence technique in exfoliated cells occurring in the pharyngeal exudate of 18 out of 29 patients with peripheral facial paralysis. the most frequently encountered antigens were: coxsackie a and b virus (33.3%), adenovirus (16.7%), and the association coxsackie b virus + adenovirus (16.7%). the possibility that some of the so-called "idiopathic" peripheral facial paralyses may have a viral etiology is discussed. | 1985 | 3004020 |
| [pathogenesis of virus-induced diabetes (a review of the literature)]. | 1985 | 3004041 | |
| coxsackievirus b-3 myocarditis. identification of different pathogenic mechanisms in dba/2 and balb/c mice. | dba/2 and balb/c mice, both h-2d, develop myocardial inflammation and necrosis when infected with a heart-adapted strain of coxsackievirus group b, type 3. similar inoculation of c57bl/6 (h-2b) animals results in minimal myocarditis despite equivalent heart virus titers in the three stains. thus, the host's genetic constitution influences the pathogenesis of the infection. anti-mouse thymocyte serum and monoclonal iad antibody effectively prevent myocarditis induction in dba/2 and balb/c mice, w ... | 1986 | 3004227 |
| elimination of human enteric viruses during conventional waste water treatment by activated sludge. | the present study was undertaken to determine if viruses were selectively eliminated during waste water treatment. human enteric viruses were detected at all steps of treatment in a conventional activated sludge waste water treatment plant. liquid overlays and large volume sampling with multiple passages on bgm cells permitted the detection of poliovirus (serotypes 1, 2, and 3), coxsackievirus b (serotypes 1, 2, 3, 4, and 5), and echovirus (serotypes 3, 14, and 22), as well as reoviruses. the me ... | 1986 | 3028589 |
| immunohistochemical study of the myocardium in murine coxsackie b3 virus myocarditis using monoclonal antibodies--significance of lyt 1 antigen-bearing lymphocytes in cell-mediated immunity. | this light- and electron-microscopic study using monoclonal antibodies provides an immunohistochemical demonstration of lymphocytes in situ in the myocardium in murine coxsackie b3 virus myocarditis. on the 7th and 9th days after virus inoculation, we observed many necrotic cardiocyte foci infiltrated with numerous inflammatory cells including macrophages and t lymphocytes. there were only a few lyt 1-bearing lymphocytes in this phase of inoculation. on the 14th day there were many lyt 1-bearing ... | 1986 | 3029449 |
| myocarditis with myocardial infarction like findings in a 3-year old girl. | a 3 year-old japanese girl had an acute onset associated with vomiting. the electrocardiogram (ecg) indicated changes similar to those of acute myocardial infarction (mi); there was no past history of kawasaki disease. selective coronary angiography taken on the 28th day of illness revealed no abnormality. thallium 201 scintigraphy was also performed and it revealed that the area of absent myocardial uptake was in the anterior wall. in serological findings, antibody titers against coxsackie b-3 ... | 1986 | 3029450 |
| the role of the cd8-positive subset of t cells in proliferative responses to soluble antigens. ii. cd8-positive cells are not responsible for dr-associated differences in responsiveness to mumps and coxsackie b4. | the role of cd8 (t8, leu 2)-positive t lymphocytes in the proliferative t-lymphocyte response to mumps and coxsackie b4 viral antigens in vitro was investigated. the frequency among enriched t-lymphocyte blasts of antigen-reactive t lymphocytes (artl) restricted by different dr-associated elements was investigated, using antigenic restimulation with allogeneic antigen-presenting cells in a limiting dilution assay. a decreased frequency of dr3-restricted and an increased frequency of dr4-restrict ... | 1986 | 3010444 |
| variations in the susceptibility to coxsackievirus b3-induced myocarditis among different strains of mice. | this study was undertaken to examine the inherent predisposition of different inbred strains of mice to develop coxsackievirus b3-induced myocarditis. a time course study established the pertinent, differential parameters of the disease and their corresponding genetic control. the a.by/snj (h-2b), a.sw/snj (h-2s), a.ca/snj (h-2f), b10.s/sgsf (h-2s), b10.pl/sgsf (h-2u), and c3h.nb/snj (h-2p) strains were found to vary widely in the extent and duration of viremia, in the temporal appearance and ti ... | 1986 | 3005402 |
| coxsackie b virus-specific igm responses in coronary care unit patients. | an enzyme-linked immunosorbent assay (elisa) test using polyvalent antigens and antisera was used to detect coxsackie b virus-specific igm responses in 329 patients admitted to the coronary care unit, wellington hospital, new zealand over a 12-month period. the sera of 30 of 153 (19.6%) patients with acute myocardial infarction (ami), 16 of 98 (18.4%) with chest pain, and 7 of 46 (15.2%) patients with arrhythmia were positive for coxsackie b virus-specific igm. four of 12 (25%) patients with hea ... | 1986 | 3005490 |
| [comparison of serous meningitis morbidity and the concentration of the coxsackie b virus in sewage]. | the paper shows that the frequency of detection and concentration of coxsackie b virus in sewage correlate with the number of patients and asymptomatic virus excretors. | 1986 | 3008437 |
| severe pancreatitis and fatty liver progressing to cirrhosis associated with coxsackie b4 infection in a three year old with alpha-1-antitrypsin deficiency. | a 3-year-old girl in whom severe acute pancreatitis was associated with evidence of coxsackie b4 virus infection was alpha-1-antitrypsin deficient. lack of this modulator of proteolysis may have been responsible for her severe course. fatty liver at presentation progressed to cirrhosis in the ensuing 18 months. | 1986 | 3008494 |
| [cloning of coxsackie virus b 3]. | 1986 | 3008770 | |
| rapid identification of coxsackie b viruses after immunoprecipitation and nucleic acid hybridization. | to circumvent the difficulties in concentrating sufficient virus from a clinical or environmental sample for detection and identification, we have used immunoprecipitation to rapidly concentrate coxsackie b viruses from both large and small sample volumes. antiviral serum and killed staphylococcus aureus cells as a protein a source were used to bind and collect the virus. radioactively-labeled viral nucleotide sequences were used to identify the collected virus by nucleic acid hybridization. the ... | 1986 | 3009085 |
| detection of coxsackie-b-virus-specific rna sequences in myocardial biopsy samples from patients with myocarditis and dilated cardiomyopathy. | full-length virus genomic rna (7.4 kilobases; kb) was isolated from coxsackie b2 virus purified from infected monkey kidney cells in culture. dna complementary to 6.3 kb of virus rna was prepared by reverse transcription and cloned in a plasmid vector. a 1.6 kb coxsackie-b-virus-specific dna clone derived from the conserved 3' region of the virus genome was used as a hybridisation probe to test for the presence of virus nucleic acid sequences in myocardial biopsy samples. positive hybridisation ... | 1986 | 2871380 |
| persistent coxsackie b virus specific igm response in patients with recurrent pericarditis. | 1986 | 2873293 | |
| persistence of coxsackie b virus-specific igm. | 1986 | 2874305 | |
| coxsackievirus b4 heterogeneity: effect of passage on neutralization and mortality. | we have compared two cb4 isolates for virulence, tissue tropism, and antigenic drift using monoclonal antibodies. both isolates replicated in c57b1/6 and balb/c mice. the human isolate edwards, recovered from a fatal case of encephalohepatomyocarditis, produced lethal infection in adult animals. lethal infections were associated with high viral titers in visceral organs but not with the presence of specific neutralizing epitopes. virulence seemed stable upon passage, and also the avirulent jvb i ... | 1986 | 2881467 |
| [the hla system and coxsackie b viral myocarditis in adults]. | the distribution of hla antigens a, b and c was studied in 152 normal donors, 53 patients with coxsackie b virus myocarditis, 35 patients with myocarditis of unknown origin, 16 coronary patients and 14 rheumatic patients with high titres of anti-coxsackie virus antibodies. coxsackie virus myocarditis was associated with increased occurrence of hla antigens a3, b40, cw2 and a28, the increase being significant (with an adjustment to the number of the tested antigens) for a3 in severe and medium my ... | 1986 | 3031353 |
| [clinical picture of coxsackie b virus myocarditis in children]. | 1986 | 3035473 | |
| biochemistry and pathogenicity of echovirus 9. ii. mutants of echovirus 9, strain hill, with altered capsid surface properties. | the two echovirus 9 strains hill and barty have been shown previously not only to differ in pathogenicity for newborn mice but also in a number of in vitro characteristics which depend on viral capsid structure. three spontaneously occurring mutants of the mouse-apathogenic echovirus 9 prototype strain hill being resistant to an inhibitor of plaque formation present in agar were isolated and compared biochemically and biophysically to their parent strain and to the mouse-pathogenic echovirus 9 s ... | 1986 | 3739228 |
| isolation of echo virus type-22 from a child with acute myopericarditis--a case report. | 1986 | 3744414 | |
| [does virus diagnosis open new ways for the classification and treatment of sudden deafness, unilateral vestibular loss and idiopathic facial paralysis?]. | paired sera from 51 patients with sudden deafness, 21 patients with acute vestibular loss and 28 patients with bell's palsy were examined for antibodies against mumps-, echo-, herpes simplex- and coxsackie viruses. only two serum samples showed a significant titer increase or decrease. so viral etiology of sudden deafness, acute vestibular loss and bell's palsy seems to be unlikely and does not change the assignment and therapy of this disease. | 1986 | 3771296 |
| in vitro and in vivo anti-picornavirus activity of some p-benzoylphenoxypyridines. | fifteen p-benzoylphenoxypyridines were initially evaluated for their in vitro activity against rhinoviruses (rv) 1a, 2 and 64 and coxsackie virus (cox) a21 and for their oral prophylactic and therapeutic activity in swiss albino mice lethally challenged with cox a21. one compound, (4-[(5-methylsulfonyl-2-pyridinyl)oxy]phenyl) phenyl methanone, was selected for additional evaluation. these studies showed the compound to possess mic50 values of less than or equal to 5 micrograms/ml against only 6 ... | 1986 | 3777916 |
| risk factors associated with echovirus 11' infection in a hospital nursery. | an outbreak of nosocomial echovirus 11' illness occurred between july 22 and august 12, 1983, infecting 14 infants in the nurseries at a hospital. stool, throat or cerebrospinal fluid specimens for viral isolation were obtained from 142 and serum pairs from 98 of the 192 infants exposed to the nurseries during the outbreak. infection was determined by isolation of virus from stool or cerebrospinal fluid and/or a 4-fold rise in neutralizing antibody to echovirus 11'. eight infants were severely i ... | 1986 | 3952008 |
| optimization of the bgm cell line culture and viral assay procedures for monitoring viruses in the environment. | an in-depth study of the continuous cell line designated bgm is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, sera, nahco3 levels, culture bottles, cell concentration, overlay media, agar, virus infection conditions, and cell-dissociating agents. additionally, a comparative virus isolation ... | 1986 | 3010860 |
| coxsackie virus b4 produces transient diabetes in nonhuman primates. | cynomolgus, rhesus, and cebus monkeys failed to show glucose tolerance or insulin secretion abnormalities after infection with encephalomyocarditis virus or coxsackie virus b4. patas monkeys also showed no abnormalities after infection with encephalomyocarditis virus. however, patas monkeys infected with coxsackie virus b4 or treated first with a subdiabetogenic dose of streptozocin and then infected sequentially with coxsackie viruses b4 and b3 showed transient elevation of glucose tolerance te ... | 1986 | 3011574 |
| coxsackievirus b2 infection in a neonate with incontinentia pigmenti. | because of the concern for herpes simplex virus infection in the neonate, the presence of neonatal vesiculobullous lesions is a critical finding. however, there are other etiologies for these lesions. a case of a neonate with a vesicular rash and meningoencephalitis which was initially thought due to herpes is presented. the infant was ultimately determined to have incontinentia pigmenti and a concomitant coxsackievirus b2 infection. | 1986 | 3012453 |
| effects of viral infection on contraction of the diaphragm in mice. | isometric contractile properties of isolated phrenic nerve-diaphragm muscle preparations were used to study the effects of picornavirus infections on diaphragm muscle function. properties of muscles from virus-inoculated and control mice were similar during brief contractions. however, when subjected to a series of fatiguing contractions by indirect or direct stimulation, muscles of mice inoculated with a paralytic variant of encephalomyocarditis (emc) virus showed a greater rate of fatigue and ... | 1986 | 3012575 |
| genetic predisposition to diabetes mellitus is associated with impaired humoral immunity to coxsackievirus b4. | experiments were performed to determine whether genetic predisposition to diabetes mellitus (dm) or clinical dm or both exert an influence on the production of neutralization antibodies to coxsackievirus b4 (cb4). the homozygous diabetic mutant mouse db+/db+, on the inbred c57bl/ksj genetic background, develops a diabetes-like disease when maintained on ad libitum diet but restriction of excess food intake prevents overt disease. the doubly heterozygote db+/+m or the homozygote +m/+m misty coat ... | 1986 | 3013555 |
| coxsackie b virus-specific igm antibody and myocardial infarction. | the elisa technique was shown to be group-specific for the detection of igm antibodies against coxsackie b viruses, and probably against a wider range of enteroviruses. no evidence was obtained that recent coxsackie b-virus infection predisposes to myocardial infarction. | 1986 | 3014150 |
| evaluation of mixed cell types and 5-iodo-2'-deoxyuridine treatment upon plaque assay titers of human enteric viruses. | four continuous cell lines, bgm, l-132, hel-299, and rd, were compared both when cultured separately and as mixtures for use in plaque assay titrations of human adenovirus 1 and six human enterovirus serotypes. the effect of incubating these cell cultures in media containing 5-iodo-2'deoxyuridine (idu) prior to inoculation with virus was also studied. the use of mixed-cell cultures revealed cell line-dependent synergistic effects as well as inhibitory effects. these effects were strongly virus d ... | 1986 | 3015021 |
| recovery of viruses from water by a modified flocculation procedure for second-step concentration. | a reduction in virus recovery efficiencies stemming from a change in the commercial processing of powdered beef extract was reversed by the addition of celite analytical filter aid. supplementing beef extract with this silicate is recommended as a modification to the organic flocculation procedure for second-step concentration in monitoring for waterborne viruses. considerable differences in virus recovery were found among lots of beef extract and celite preparations; this indicates that the per ... | 1986 | 3015024 |
| coxsackie b4 virus induces short-term changes in the metabolic functions of mouse pancreatic islets in vitro. | mouse pancreatic islets cultured in vitro were infected with a tissue culture-adapted or a mouse pancreas-adapted strain of coxsackie b4 (cb4) virus. the effects of the viruses on the islets were assessed by examination of their biochemical functions. it was found that the mouse pancreas-adapted strain of cb4 induced a 'leakage' of insulin from islets incubated at a basal (2 mmol l-1) glucose concentration, both at two and four days following infection. however, at a stimulatory concentration of ... | 1986 | 3015449 |
| susceptibility of endothelial cells derived from different blood vessels to common viruses. | we examined whether endothelial cells derived from different blood vessels vary in their susceptibility to viral infection. five common viral pathogens of humans (herpes simplex 1, measles, mumps, echo 9, and coxsackie b4 viruses) were evaluated for growth in endothelial cells derived from bovine fetal pulmonary artery, thoracic aorta, and vena cava. all five viruses replicated in each type of endothelial cell. there were apparent differences in the quantities of measles and mumps viruses produc ... | 1986 | 3015862 |
| concurrent cytomegalovirus and coxsackie b virus infections in a heart-lung transplant recipient. | a 35-year-old woman with fibrosing alveolitis was given a combined heart and lung transplant at papworth hospital, cambridge. although her immediate post-operative course was satisfactory, she subsequently experienced concurrent primary cytomegalovirus and coxsackie b virus infections associated with significant morbidity including pancreatitis. she made a full recovery and has remained well for more than 2 years after transplantation. | 1986 | 3016100 |
| mu-antibody capture elisa for the rapid diagnosis of enterovirus infections in patients with aseptic meningitis. | between august and november 1985, 45 patients with suspected aseptic meningitis were investigated using conventional virus isolation procedures and the mu-antibody capture coxsackie b igm enzyme-linked immunoabsorbent assay (elisa) test, which is well known to cross-react with other members of the enterovirus group. an enterovirus was isolated from 22% of patients compared with 67% who were positive in the elisa test. not only was the rate of enterovirus detection increased by using this elisa m ... | 1986 | 3016164 |
| humoral immune response in hereditary and overt diabetes mellitus. | the inbred diabetic mutant mouse, c57bl/ksj db +/db + (db +/db +), spontaneously develops diabetes mellitus when allowed food ad libitum. however, restriction of food intake prevents the expression of this genetic predisposition for diabetes. this experimental design has been used previously to demonstrate a deficient neutralizing antibody response to coxsackievirus b4 (cb4) in mutants with the genetic predisposition only. these observations demonstrate that in the genetically predisposed diabet ... | 1986 | 3016167 |
| demonstration of suppressor cells in coxsackievirus group b, type 3 infected female balb/c mice which prevent myocarditis. | coxsackievirus group b type 3 (cvb3) induces myocarditis in male balb/c mice but produces little cardiac injury in females. males develop cytolytic t lymphocytes (ctl) reactive to heart antigens which primarily cause the inflammation and cardiac injury observed in the disease. infected female mice lack this ctl response because they rapidly produce suppressor cells inhibiting both cellular immunity and cardiac inflammation. four lines of evidence demonstrate suppressor cells in females. first, f ... | 1986 | 3017580 |
| involvement of natural killer cells in coxsackievirus b3-induced murine myocarditis. | the role of natural killer cells in the temporal development of coxsackievirus b3-induced myocarditis in adolescent cd-1 male mice was examined. inoculation of purified cvb3m induced maximum nk cell activity in the splenic populations at 3 days postinoculation (p.i.) as assessed by lysis of yac-1 cells; maximum virus titers in heart tissues were also found at day 3 p.i. mice depleted of nk cells after injection of anti-asialo gm1 antiserum i.v. had decreased nk cell activity, increased cvb3m tit ... | 1986 | 3018079 |
| protein kinase in nondiabetogenic coxsackievirus b4. | alkali-dissociated, purified preparations of prototype coxsackievirus b4 release a protein kinase that catalyzes the incorporation of gamma-phosphate from 32p-labeled atp into three virus capsid proteins (vp1, vp3, vp4), several additional proteins of the particle, and exogenous acceptor proteins. using protamine sulfate as an acceptor protein, we detected nearly 20-fold more enzyme activity in membrane-bound virions (mbv) than in virions of the virus. the activity in the mbv is cyclic nucleotid ... | 1986 | 3018140 |
| coxsackievirus b3 infection alters plasma membrane of neonatal skin fibroblasts. | replication of coxsackievirus b3 occurred for days in cultures of murine neonatal skin fibroblasts in the absence of cytopathology and resulted in alteration of the plasma membrane. dual immunofluorescence studies showed that the lectin ulex europaeus agglutinin i bound only to cells producing viral capsid antigens. cultures of coxsackievirus b3-inoculated murine neonatal skin fibroblasts showed maximum binding of this lectin at 72 h postinoculation. these data show that in a nonlytic infection ... | 1986 | 3018294 |
| [experimental nephritis induced by coxsackie b4 virus in mice--transient mesangial proliferation associated with acute viremia]. | 1986 | 3018334 | |
| [experimental models of insulin-dependent diabetes]. | 1986 | 3020804 | |
| mechanism of inactivation of enteric viruses in fresh water. | fresh water obtained from nine sources was shown to cause inactivation of poliovirus. further testing with four of these water samples showed that enteric viruses from different genera were consistently inactivated in these freshwater samples. studies on the cause of inactivation were conducted with echovirus type 12 as the model virus. the results revealed that the virucidal agents in the waters tested could not be separated from microorganisms. any treatment that removed or inactivated microor ... | 1986 | 3021056 |
| enumeration of enterovirus particles by scanning electron microscopy. | enumeration of virus particles requires relatively concentrated and uniformly dispersed virus preparations, which is difficult to achieve by the usual methods of negative staining and transmission electron microscopy. we have developed an electrophoretic method that concentrates enteroviruses onto a polycarbonate membrane for examination by high-resolution scanning electron microscopy. the electrophoretic apparatus comprises three chambers in electrical series, each containing 3.5 ml of dilute b ... | 1986 | 3021798 |
| coxsackievirus group b replication in cultured fetal baboon aortic smooth muscle cells. | all six coxsackievirus b (cvb) serotypes replicated to various extents in fetal baboon aortic smooth muscle cells (smc) in culture. cvb3 and cvb4 replicated to the highest titers and induced no cytopathology at the level of light microscopy. maximum yields of cvb3 were produced between 12 and 24 hr postinoculation. up to 15% of smc cells became infected, as determined by immunofluorescence assays with anti-cvb3 antiserum, yet overall cell division in infected cultures did not differ from infecte ... | 1986 | 3021897 |
| [late sequelae after coxsackie virus b myocarditis]. | 1986 | 3022417 | |
| liquefactive necrosis in coxsackie b encephalitis. | coxsackieviruses may cause serious illness in infants and children, specifically myocarditis and meningoencephalitis. central nervous system lesions have been characterized as inflammatory in nature with mononuclear cell infiltration, neuronophagia, and glial nodule formation largely confined to the brain stem and spinal cord. we present two infants with documented coxsackie b virus infection who also had widespread multifocal areas of liquefaction necrosis unassociated with inflammation. such a ... | 1986 | 3022671 |
| [the pathological observation of experimental infections by coxsackie virus b4 (cvb4) in mice]. | 1986 | 3022952 | |
| coxsackievirus b-3 myocarditis. t-cell autoimmunity to heart antigens is resistant to cyclosporin-a treatment. | a cardiotropic variant of coxsackievirus group b, type 3 (cvb3) induces myocarditis in inbred balb/c mice. myocardial injury is predominantly mediated by t lymphocytes recognizing normal myocyte antigens, making this an autoimmune disease. nonetheless, the autoimmune response cannot be inhibited by cyclosporin a (csa) treatment of the infected animals. mortality in treated mice was increased 2-4 times, but neither virus-specific antibody or cytolytic t-lymphocyte responses were affected, and max ... | 1986 | 3024491 |
| myocarditis in mice infected with coxsackie virus b3. | perimyocarditis in the heart of balb/c mice infected with coxsackie virus group b type 3 (cb3) was studied to determine whether it is limited to the right perimyocardium and to show whether or not perimyocarditis or myocardial lesions are produced in both left and right ventricles. cb3 was recovered from the heart on days from 2 to 13 after inoculation, but thereafter no virus was isolated from any part of the heart. histopathologically, from days 1 to 4, hyaline or granular degeneration and nec ... | 1986 | 3024693 |
| a microneutralization test for the identification of enterovirus isolates. | two-hundred and thirty-four enterovirus isolates were identified using a recently modified, microneutralization procedure. neutralization tests were performed in 96 well plastic 'v' plates, using 20 microliter quantities of antisera and virus, then inoculated onto monolayers of buffalo green monkey kidney cells, growing in 48 well tissue culture plates. utilization of this microneutralization procedure resulted in considerable savings of time, material, and particularly, neutralizing antisera. | 1986 | 3025242 |
| [pathogenesis of atrial myocarditis in monkeys inoculated with cb1oxsackie virus b3]. | 1986 | 3025310 | |
| [neonatal myocardial infarct associated with maternal-fetal infection due to coxsackie b4 virus]. | heart failure with myocardial infarction occurred in the course of a neonatal infection with aseptic meningitis. coxsackie b4 infection was found in the child and its mother. the unusual myocardial action of the virus, coronary embolus, intermediate role of a pancreatitis. by age 8 months, under digitalis glycosides treatment, growth and psychomotor development were normal. | 1986 | 3026276 |
| adult onset still's disease or coxsackie polyarthritis? | the clinical manifestations and laboratory findings of two patients with a presumptive diagnosis of coxsackie b4 virus infection are described. a striking feature was the similarity with adult onset still's disease, with spiking fever, evanescent macular rash, and severe polyarthritis. this latter feature persisted for many weeks and required steroids to control the symptoms. review of the literature has supported the proposition that many cases of adult onset still's disease may be due to coxsa ... | 1986 | 3026295 |
| acquired immunity and allergy of cells cultured in vitro. | it is in the reinoculated cell line fl that the sensitivity of cells under the action of a small dose of diphtheria toxin (dt) changes to the toxin itself as well as to the coxsackie enterovirus. (b-5). after surviving the infection and getting again "healthy", the cells have acquired simultaneously an elevated sensibility to dt and reduced susceptibility to b-5 transmissible even in long passages. these acquired properties are a transient phenomenon, after a certain number of passages the cultu ... | 1986 | 3027165 |
| persistent infection of rat insulinoma cells with coxsackie b4 virus. brief report. | plaque purified coxsackie b4 virus (cb4) caused a persistent infection of rat insulinoma (rin) cells which lasted for the 70 day observation period. infectious virus was produced and no cytopathic effect was observed. indirect immunofluorescence and infectious center assays demonstrated that a majority of rin cells were infected. defective interfering particles were not demonstrated. despite persistent infection, insulin synthesis by rin cells was not altered. | 1986 | 3002301 |
| monoclonal antibody that inhibits infection of hela and rhabdomyosarcoma cells by selected enteroviruses through receptor blockade. | balb/c mice were immunized with hela cells, and their spleen cells were fused with myeloma cells to produce hybridomas. initial screening of culture fluids from 800 fusion products in a cell protection assay against coxsackievirus b3 (cb3) and the cb3-rd virus variant yielded five presumptive monoclonal antibodies with three specificities: protection against cb3 on hela, protection against cb3-rd on rhabdomyosarcoma (rd) cells, and protection against both viruses on the respective cells. only on ... | 1986 | 3003376 |
| characterization of an echovirus type 30 variant isolated from patients with aseptic meningitis. | an outbreak of echovirus type 30 infection associated with aseptic meningitis occurred among newborn babies in a hospital neonatal room at fukui city in 1983. the isolated virus was identified as an antigenic variant of echovirus type 30 by cross-neutralization tests with antisera against the prototype bastianni strain and the present isolate. western blot analysis demonstrated that the antigenic determinants responsible for virus neutralization, some of which were type specific and others strai ... | 1986 | 2425228 |
| humoral immunity against viral antigens in type 1 diabetes: altered iga-class immune response against coxsackie b4 virus. | a group of 210 pediatric type 1 diabetic patients with long duration of illness and their matched controls (age range 2-19 years) were analysed for coxsackie b4 antibodies in igg-, igm- and iga-antibody classes by enzyme-linked immunosorbent assay (elisa). about 60% of both patients and controls were seropositive. however, patients had higher prevalence and mean levels of iga-class antibodies compared to controls. no such difference was found in igg- or igm-antibody classes. the elevation of iga ... | 1986 | 2426921 |
| antigen-specific suppressor t cells prevent cardiac injury in balb/c mice infected with a nonmyocarditic variant of coxsackievirus group b, type 3. | two variants of coxsackievirus group b, type 3 (cvb3o and cvb3m) infect balb/c cardiac tissue equally, but only one variant (cvb3m) induces myocarditis. various studies indicate that disease resistance in cvb3o-infected mice results from generation of suppressor cells. low-dose cyclophosphamide (50 mg/kg body weight) treatment enhances inflammation in both cvb3m and cvb3o-infected animals, which clearly demonstrates that cvb3o has the capacity to induce disease, but inhibitory factors must exist ... | 1986 | 2432792 |
| [experimental diabetes in mice infected with coxsackie viruses]. | the influence of coxsackie b4 and ai3 viruses on the pancreas of mice (resistant and susceptible to diabetes) was studied. glucose intolerance and changes in the synthesis of immunoreactive insulin were detected in all the treated groups of animals. biochemical changes were more prominent in male dba/2 mice, infected with coxsackie b4 virus, in fi (cba x c57bl/6) hybrids and in female dba/2 mice infected with coxsackie ai3 virus and alloxan. | 1987 | 3028528 |
| royal free disease: perplexity continues. | 1987 | 3028544 | |
| effect of astragalus membranaceus injecta on coxsackie b-2 virus infected rat beating heart cell culture. | 1987 | 2827966 | |
| [morphofunctional changes in a culture of pancreatic islet cells from newborn piglets as affected by coxsackie b3 viruses]. | the direct influence of coxsackie virus b-3 and its selected variants abent+ and abent- on the function and structure of neonatal pig pancreatic islet cells in monolayer cultures was studied. the viruses under study possessed cytopathogenic activity and were capable of reproduction. the abent- variant of coxsackie virus b-3 possessed the most pronounced cytopathogenic activity. direct correlation between virus reproduction and insulin concentration in a culture medium was established. | 1987 | 2821534 |
| evidence for the role of a human intestinal adenovirus in the pathogenesis of coeliac disease. | we previously noted a region of amino acid sequence homology between a-gliadin, a major alpha-gliadin component known to activate coeliac disease, and the early region e1b protein of human adenovirus serotype 12 (ad12), an adenovirus isolated from the human intestinal tract. in the present study sera from coeliac disease patients from the united kingdom and the united states were assayed for neutralising antibody to ad12 as evidence of past exposure to that virus and for antibody to synthetic pe ... | 1987 | 2822550 |
| the histopathology of enterovirus infections of new-born mice. | for 32 years we have provided a routine histopathological service on animals for the regional virus laboratory, ruchill hospital, glasgow. this article concerns mainly experimental coxsackievirus a, coxsackievirus b and echovirus infections in new-born mice. in addition to previously reported findings, we have sometimes observed degenerative and inflammatory changes in neurons related to the gasserian, posterior root or autonomic ganglia. not all deposits of brown fat are simultaneously the seat ... | 1987 | 2822811 |
| a novel presentation of coxsackie b2 virus infection during pregnancy. | a 39-year-old woman presented at 34 weeks gestation with a febrile illness. this initially settled but recurred 6 days later, when uncontrolled vaginal bleeding necessitated caesarian section. at operation, vesicles were seen on the mother's hyperaemic uterus. the infant developed tachypnoea, bradycardia, cerebral irritation, an enlarged liver and spleen, intravascular coagulation and a transient rash. mother and child made a full recovery. coxsackie b2 virus was isolated from the infant and ris ... | 1987 | 2822813 |
| coxsackie b2 virus causing simultaneous hand, foot and mouth disease and encephalitis. | 1987 | 2822814 | |
| possible mechanisms in the pathogenesis of virus-induced diabetes mellitus. | insulin-dependent diabetes mellitus results from destruction of pancreatic beta cells. viruses and autoimmunity have been implicated as possible causes of beta cell destruction in genetically predisposed individuals. the evidence for viruses comes largely from experiments in animals, but several studies in humans point to viruses as triggers in the pathogenesis of diabetes in some cases. in animal models, at least 4 different possible mechanisms for virus-induced diabetes have been proposed. the ... | 1987 | 2824113 |
| cardiac myosin and autoimmune myocarditis. | infection with type 3 of the group b coxsackieviruses (cb3) sometimes leads to the development of myocarditis in humans. circumstantial evidence in the form of heart-reactive antibodies in these cases of human myocarditis suggests that the later phases of the disease may be due to autoimmunization. since human myocarditis is a relatively rare sequel to infection with cb3 virus, we propose that it reflects a genetic predisposition in some individuals. to investigate this possibility we establishe ... | 1987 | 2824143 |
| coxsackie virus infection in association with heart affection in man. | the frequency of actual infections by selected types of coxsackie virus (b1-b6, a7, a9) has been followed up in adult patients hospitalized for reason of heart disease. criteria of actual coxsackie virus infection have been defined, including a significant rise of titer of virus neutralizing antibodies and/or presence of virus-specific igm antibodies. an actual coxsackie virus infection has been found in 71 (46.3%) out of 153 patients. most frequently coxsackie b1 virus infection (24 times) and ... | 1987 | 2824602 |
| virus infection and knee injury. | serological evidence of virus infection was sought in 31 consecutive patients presenting with knee swelling and compared with age/sex-matched controls. in a normal age/sex-matched control group, 42% of patients had evidence of recent or past infection with coxsackie b virus, emphasising the care required in the evaluation of the significance of coxsackie b neutralization titres in individual patients. of 12 patients presenting with knee swelling and a history of a twisting injury, eight had sero ... | 1987 | 2825728 |
| viral infections in beta-thalassemia/hemoglobin e patients. | one hundred ten patients with beta-thal/hb e disease and 60 normal controls matched for age and socioeconomic status were followed for 1.5 years. they were examined clinically, and blood and plasma were studied for coxsackie b viruses and others. the findings suggest that the patients are more susceptible to coxsackie b virus but not to rubella, herpes simplex, cytomegalovirus, adenovirus, and m. pneumoniae. in contrast to bacterial infections, splenectomized patients did not show evidence of in ... | 1987 | 2825835 |
| the relationship between coxsackie-b-virus-specific igg responses and genetic factors (hla-dr, gm, km) in insulin-dependent diabetes mellitus. | igg antibody titres to coxsackie b1-b6 were measured in 113 insulin-dependent diabetes mellitus (iddm) patients whose mean age was 12.2 years and mean duration of iddm was 4.6 years, and in 87 normal sibling controls whose mean age was 13.8 years. compared with their normal siblings, the diabetics had a significantly increased frequency of high response (titre greater than or equal to 320) to coxsackie b2 (8% versus 1%, p = 0.028), to coxsackie b4 (15% versus 1%, p = 0.0006), and to coxsackie b ... | 1987 | 2834133 |
| study of mouse spleen "natural cytotoxic" (nc) cell activity related to in vivo administration of b4 coxsackievirus. | by using a2g 40-day-old mice as responders, the influence of in vivo infection with a human strain of b4 coxsackievirus on "natural cytotoxic" (nc) cell activity was assayed, in relation to other immunomodulating treatments (beta-interferon, interleukin-1, or prostaglandin-e2). a significant increase of nc cell cytolysis was noticed in virus-infected mouse group, as compared to the control. the nc cell stimulation exerted by in vivo administration of beta-interferon or of interleukin-1 was not m ... | 1987 | 2830715 |
| [experimental and bacterial conditioned coxsackie b virus-induced pancreatitis]. | 1987 | 2830736 | |
| natural and lectin-dependent cytotoxicity in virus-induced human myocarditis. | the human spontaneous cell-mediated cytotoxicity (scmc) and lectin dependent cell-mediated cytotoxicity (ldcmc) of peripheral blood leukocytes (pbl) were studied in patients with different forms of postviral myocarditis. the pbl cytotoxicity was evaluated using 51cr-labelled crbc (chicken red blood cells) as targets and results were expressed as a percentage of specific release. the control group was represented by 150 healthy persons of both sexes. no significant age-related differences were fo ... | 1987 | 2830855 |
| hydrops due to myocarditis in a fetus. | at 18 weeks of gestation a fetus was studied sonographically because of advanced maternal age and found to have hydrops of unknown etiology with ascites, pleural, and pericardial effusions. an abortion was performed and in the fetal/placental material myocarditis and thyroiditis were documented. maternal antibodies to coxsackie virus b5 showed a onefold rise. tentatively, the hydrops is assigned to coxsackie virus myocarditis, a lesion not previously identified in fetuses. | 1987 | 2843206 |
| cardiovascular effects of congenital infections. | in the course of gestation, many bacteria, parasites, and viruses may infect the pregnant woman, but few cross the placenta to affect the fetus and fewer still affect the fetal heart. although the incidence of fetal cardiac infection is low, the effect on the fetus is major. in terms of frequency, rubella virus, toxoplasma gondii, and coxsackie virus b are the principal infectious agents affecting the fetal heart, but any number of organisms may cross the placenta to affect the fetus. the pathog ... | 1987 | 2849958 |
| murine natural killer cells limit coxsackievirus b3 replication. | previous indirect evidence suggested that natural killer (nk) cells play a role in coxsackie virus b3 serotype 3, myocarditic variant (cvb3m)-induced myocarditis by limiting virus replication. in this study, we present direct evidence that nk cells can limit cvb3m replication both in vitro and in vivo. virus titers are lowered in primary murine neonatal skin fibroblast (mnsf) cultures incubated with activated splenic large granular lymphocytes (lgl) taken from mice 3 days postinoculation of cvb3 ... | 1987 | 3036947 |
| perinatal transmission of coxsackievirus b3 in mice. | oral infection of pregnant mice with coxsackievirus b3 (cb3) late in gestation produced maternal viremia, which peaked three to four days after challenge, then rapidly diminished. ninety percent of mice developed igg antibody to cb3 by ten days after challenge. cb3 titers from placental tissue peaked at two to four days after maternal infection, but moderate titers persisted for at least eight days. though virus could be recovered (less than 1.0-1.5 log10 pfu/g) from fetal tissue in only a small ... | 1987 | 3036959 |
| reemergence of an epidemic coxsackievirus b5 genotype. | outbreaks of coxsackievirus b5 (cb5) infections occur primarily during peak epidemic years, with comparatively few cases occurring during intervening years. this pattern of periodic cb5 epidemicity is quite distinct from the general endemicity typical of other group b coxsackieviruses. to determine the genetic relationships among cb5 isolates from different outbreaks, we compared viral rnas by ribonuclease t1 oligonucleotide fingerprinting. isolates obtained within an epidemic year had very simi ... | 1987 | 3036966 |
| the complete nucleotide sequence of coxsackievirus b4 and its comparison to other members of the picornaviridae. | the genome of the prototype stain of coxsackievirus b4 (j.v.b. benschoten) has been cloned in escherichia coli and its complete nucleotide sequence determined. excluding the poly(a) tract, the rna genome is 7395 nucleotides in length and appears to encode a single polyprotein of 2183 amino acids. the predicted amino acid sequence of the polyprotein shows close homology (88%) to that of the previously sequenced coxsackievirus b3 and to certain regions of the polyproteins of the polioviruses and h ... | 1987 | 3037008 |
| [persistent infection caused by the coxsackie b3 virus in adult mice]. | a model of persistent infection with coxsackie b-3 virus was developed in adult mice with clinical manifestations of the disease and long-term (up to 13 months) excretion of the causative agent. the method of multiple organ cultures was shown to be suitable for isolation of the persisting enterovirus. the presence of persistent infection was confirmed by the detection of igm antibody in repeated daily examinations of the animals for 4 months. it seems to be expedient to use this model for invest ... | 1987 | 3037797 |
| alteration of immune response to coxsackie b3 virus by streptozotocin in dual-aetiology diabetes mellitus in mouse. | 1987 | 3038739 | |
| cold reacting antilymphocyte antibodies in type i (insulin dependent) diabetes. | non hla antilymphocyte antibodies have been detected in numerous auto-immune illnesses and notably in type i (insulin dependent) diabetes. in order to ascertain their role in this illness, the actual frequency of this phenomenon has been estimated. the privileged cellular target has been determined. finally correlations with the other immunogenetic markers of diabetes have been investigated. non hla antilymphocyte antibodies are frequent, above all at the beginning of the illness (32.4% before 6 ... | 1987 | 3032702 |
| coxsackievirus b 1 induced murine polymyositis: acute infection with active virus is required for myositis. | although the etiology of human polymyositis (pm) remains obscure, group b coxsackieviruses (cvb) have been implicated in disease pathogenesis and a particular strain of type 1 cvb (cvb 1) has been shown to cause in mice an inflammatory myositis which is similar to human pm. after infection of neonatal swiss mice with active cvb 1, virus replicated to high titers in muscle and produced acute myonecrosis. viral titers peaked in muscle at day 7 and virus was undetectable after day 14. myocytes rege ... | 1987 | 3033235 |