Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| comparison of efficacy of ciprofloxacin and doxycycline against experimental melioidosis and glanders. | melioidosis and glanders are caused by the closely related species burkholderia pseudomallei and burkholderia mallei, respectively. whereas melioidosis is a significant cause of morbidity in south-east asia, glanders is extremely rare. the efficacies of ciprofloxacin and doxycycline were assessed against a strain of b. pseudomallei and a strain of b. mallei which were susceptible to both antimicrobials in vitro. porton outbred mice and syrian hamsters were given 40 mg/kg of either doxycycline or ... | 2000 | 10837435 |
| antibody response to burkholderia cepacia in patients with cystic fibrosis colonized with burkholderia cepacia and pseudomonas aeruginosa. | this study was designed to determine the relationship between formation of serum antibodies to lipopolysaccharide (lps) core antigen of burkholderia cepacia and pulmonary colonization with b. cepacia and pseudomonas aeruginosa in patients with cystic fibrosis (cf), and to define if an enhanced host humoral immune response to b. cepacia was related to a poor clinical outcome. | 2000 | 10841094 |
| the nramp proteins of salmonella typhimurium and escherichia coli are selective manganese transporters involved in the response to reactive oxygen. | nramps (natural resistance-associated macrophage proteins) have been characterized in mammals as divalent transition metal transporters involved in iron metabolism and host resistance to certain pathogens. the mechanism of pathogen resistance is proposed to involve sequestration of fe2+ and mn2+, cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the ... | 2000 | 10844693 |
| chronic granulomatous disease. report on a national registry of 368 patients. | a registry of united states residents with chronic granulomatous disease (cgd) was established in 1993 in order to estimate the minimum incidence of this uncommon primary immunodeficiency disease and characterize its epidemiologic and clinical features. to date, 368 patients have been registered; 259 have the x-linked recessive form of cgd, 81 have 1 of the autosomal recessive forms, and in 28 the mode of inheritance is unknown. the minimum estimate of birth rate is between 1/200,000 and 1/250,0 ... | 2000 | 10844935 |
| pseudomonas (burkholderia) pseudomallei in thailand, 1964-1967: geographic distribution of the organism, attempts to identify cases of active infection, and presence of antibody in representative sera. | the purpose of this study, initiated in 1964 and concluded in 1967, was to define the distribution of pseudomonas (now burkholderia) pseudomallei in thailand, to evaluate its importance as an etiologic agent, and to survey the presence of antibody in people that might indicate prior infection and/or contact with the microorganism. | 2000 | 10813478 |
| diagnostic and prognostic value of an immunofluorescent assay for melioidosis. | melioidosis caused by burkholderia pseudomallei is endemic in southeast asia. the clinical manifestations range from wound infections to acute septicemia. in some cases, recurrence can also occur following complete recovery. case fatality rates are high and a major factor is the delay in the culture and identification of the bacterium. an immunofluorescent assay (ifat) using whole-cell antigen for the detection of total antibodies to b. pseudomallei was tested with 650 sera. using a cut-off valu ... | 2000 | 10813488 |
| identification of a serine hydrolase as a key determinant in the microbial degradation of polychlorinated biphenyls. | the ability of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate (hopda) hydrolase (bphd) of burkholderia cepacia lb400 to hydrolyze polychlorinated biphenyl (pcb) metabolites was assessed by determining its specificity for monochlorinated hopdas. the relative specificities of bphd for hopdas bearing chlorine substituents on the phenyl moiety were 0.28, 0.38, and 1.1 for 8-cl, 9-cl, and 10-cl hopda, respectively, versus hopda (100 mm phosphate, ph 7.5, 25 degrees c). in contrast, hopdas bearing chlorine ... | 2000 | 10821847 |
| immunization with a pseudomonas aeruginosa elastase peptide reduces severity of experimental lung infections due to p. aeruginosa or burkholderia cepacia. | pseudomonas aeruginosa and burkholderia cepacia produce metalloproteases that effect lung injury. two epitopes (peptides 15 and 42) previously identified on p. aeruginosa elastase induce the production of antibodies that neutralize protease activity. the effects of immunization with synthetic peptides based on these epitopes on experimental lung infections due to p. aeruginosa or b. cepacia were examined. rats were immunized with peptides conjugated to keyhole limpet hemocyanin or tetanus toxoid ... | 2000 | 10823769 |
| field investigations of bacterial contaminants and their effects on extended porcine semen. | field investigations (n=23) were made over a 3-yr period at north american boar studs and farms in which the primary complaint was sperm agglutination in association with decreased sperm longevity of extended semen, and increased regular returns to estrus and/or vaginal discharges across parity. microscopic examination of extended semen from these units revealed depressed gross motility (usually <30%), sperm agglutination, and sperm cell death occurring within 2 d of semen collection and process ... | 2000 | 10798493 |
| polyhydroxyalkanoate accumulation in burkholderia sp.: a molecular approach to elucidate the genes involved in the formation of two homopolymers consisting of short-chain-length 3-hydroxyalkanoic acids. | burkholderia sp. accumulates polyhydrox-yalkanoates (phas) containing 3-hydroxybutyrate and 3-hydroxy-4-pentenoic acid when grown on mineral media under limited phosphate or nitrogen, and using sucrose or gluconate as a carbon and energy source. solvent fractionation and nmr spectroscopic characterization of these polyesters revealed the simultaneous accumulation of two homopolyesters rather than a co-polyester with random sequence distribution of the monomers [valentin he, berger pa, gruys kj, ... | 2000 | 10803903 |
| synthesis of unnatural amino acids via suzuki cross-coupling of enantiopure vinyloxazolidine derivatives. | [formula: see text] (r and s)-alpha-amino alcohols and alpha-amino acids, including 4-methoxyhomophenylalanine, with a variety of unnatural side chains have been synthesized via palladium-catalyzed cross-coupling suzuki reactions. the key building blocks 1 and 2, synthesized from the common achiral precursor 2-butene-1,4-diol, were made enantiopure utilizing a pseudomonas cepacia lipase-catalyzed kinetic resolution. the optimal conditions for the suzuki cross-coupling and the subsequent oxidatio ... | 2000 | 10804561 |
| kinetic studies of the production of nitric oxide (no) and tumour necrosis factor-alpha (tnf-alpha) in macrophages stimulated with burkholderia pseudomallei endotoxin. | the mechanism by which burkholderia pseudomallei survives in macrophages is not clearly understood. in this study, we demonstrated that the mouse macrophage cell line (raw 264.7) treated with lipopolysaccharide (lps) from b. pseudomallei (bp-lps) produced significantly less no and tnf-alpha compared with those stimulated with the lps from escherichia coli and salmonella typhi. the time required for the bp-lps to trigger substantial no and tnf-alpha release was at least 30 min, compared with < 5 ... | 2000 | 11122236 |
| properties of the trihydroxytoluene oxygenase from burkholderia cepacia r34: an extradiol dioxygenase from the 2,4-dinitrotoluene pathway. | burkholderia cepacia r34 mineralizes 2,4-dinitrotoluene via an oxidative pathway. the initial steps in the degradative pathway lead to formation of 2,4,5-trihydroxytoluene, which serves as the substrate for the ring cleavage dioxygenase. the trihydroxylated substrate differs from the usual substituted catechols found in pathways for aromatic compound degradation. to determine whether the characteristics of the trihydroxytoluene oxygenase reflect the unusual ring cleavage substrate of the 2,4-din ... | 2000 | 10795678 |
| synthesis of neoglycoproteins containing d-glycero-d-talo-oct-2-ulopyranosylonic acid (ko) ligands corresponding to core units from burkholderia and acinetobacter lipopolysaccharide. | glycal esters of kdo derivatives were converted into 2,3-anhydro intermediates, which were transformed into d-glycero-d-talo-oct-2-ulopyranosylonic acid (ko), as well as 3-o- and 4-o-p-nitrobenzoyl-ko derivatives. the exo-allyl orthoester derivative, methyl [5,7,8-tri-o-acetyl-4-o-(4-nitrobenzoyl)-2,3-o-[(1-exo-allyloxy)-ethylidene]-d-glycero-beta-d-talo-oct-2-ulopyranos]onate, prepared from the 4-o-pnbz-protected ko derivative, was elaborated into the alpha-ko allyl ketoside, the reducing disac ... | 2000 | 11128584 |
| the treatment of respiratory pseudomonas infection in cystic fibrosis: what drug and which way? | pseudomonas aeruginosa is a non-capsulate and non-sporing gram-negative bacillus that most commonly affects the lower respiratory system in humans. burkholderia (previously pseudomonas) cepacia has emerged as an important respiratory pathogen in patients with cystic fibrosis (cf). the ability of p. aeruginosa to persist and multiply in moist environments and equipment, such as humidifiers in hospital wards, bathrooms, sinks and kitchens, maybe of importance in cross-infection. p. aeruginosa infe ... | 2000 | 11129122 |
| monoclonal antibody-based rapid identification of burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. | a monoclonal antibody-based latex agglutination (mab-la) test was employed for the rapid identification of burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. these patients were admitted to 12 hospitals in the northeastern part of thailand which is a region known to be endemic for melioidosis. blood samples were collected and immediately added to the blood culture bottles which were incubated in either automated (five hospitals) or manual (seven h ... | 2000 | 11129718 |
| regioselective enzymatic aminoacylation of lobucavir to give an intermediate for lobucavir prodrug. | synthesis of lobucavir prodrug, l-valine, [(1s,2r,3r)-3-(2-amino-1,6-dihydro-6-oxo-9h-purin-9-yl)-2-(hydroxymethyl) cyclobutyl]methyl ester monohydrochloride (bms 233866), requires regioselective coupling of one of the two hydroxyl groups of lobucavir (bms 180194) with valine. either hydroxyl group of lobucavir could be selectively aminoacylated with valine by using enzymatic reactions. n-[(phenylmethoxy)carbonyl]-l-valine, [(1r,2r,4s)-2-(2-amino-6-oxo-1h-purin-9-yl)-4-(hydroxymethyl)cyclobutyl] ... | 2000 | 11131158 |
| infections in e-beta thalassemia. | infection is a major complication and the leading cause of death in thalassemia, especially e-beta thalassemia. the spectrum of infections in e-beta thalassemia include mild and severe infections, therapy-related infections such as yersinia enterocolitica infection associated with desferrioxamine (dfo) therapy, and transfusion-transmitted disease, as well as unique infections such as with pythiosis. prospective studies in thailand indicate that patients with e-beta thalassemia had more frequent ... | 2000 | 11132234 |
| [bact-alert system for hemocultures: evaluation of terminal subcultures]. | few laboratory microbiological procedures are as important as the isolation of microorganisms from blood. to evaluate the usefulness of the terminal subcultures, 5669 blood cultures giving negative results after 7 days of incubation in the bact/alert system (organon teknika) were studied. bottles were distributed as follows: 1562 adult aerobic bottles, 119 adult anaerobic bottles, 3960 pediatric bottles and 28 fan bottles. from 5669 blood cultures, 10 subcultures that yielded growth had not been ... | 2000 | 11149151 |
| cloning and characterization of estc from burkholderia gladioli, a novel-type esterase related to plant enzymes. | by screening a genomic library of burkholderia gladioli (formerly pseudomonas marginata) for clones exhibiting esterolytic activity, the gene for a novel-type esterase (estc) showing significant homology to plant enzymes could be isolated. high homology was found to two hydroxynitrile lyases originating from hevea brasiliensis (tropical rubber tree) and manihot esculenta (cassava), and to two proteins from oryza sativa (rice) that are specifically induced upon infection by pseudomonas syringae p ... | 2000 | 11152069 |
| recent developments in pulmonary infections. | 2000 | 11153574 | |
| pseudomonas antimicrobica attafuah and bradbury 1990 is a junior synonym of burkholderia gladioli (severini 1913) yabuuchi et al. 1993. | comparison of the 16s rdna sequence of pseudomonas antimicrobica lmg 18920t with published 16s rdna sequences from other pseudomonads indicated that pseudomonas antimicrobica belongs to the genus burkholderia, with burkholderia gladioli, burkholderia glumae and burkholderia plantarii as its closest neighbours. dna-dna hybridizations confirmed that pseudomonas antimicrobica and burkholderia gladioli represent the same species. strain lmg 18920t and other burkholderia gladioli strains were also in ... | 2000 | 11155989 |
| rotation and restricted use of antibiotics in a medical intensive care unit. impact on the incidence of ventilator-associated pneumonia caused by antibiotic-resistant gram-negative bacteria. | to test the hypothesis that a new program of antibiotic strategy control can minimize the incidence of ventilator-associated pneumonia (vap) caused by potentially antibiotic-resistant microorganisms, we performed a prospective before-after study in 3, 455 patients admitted to a single intensive care unit over a 4-yr period. regarding the bacterial ecology and the increasing antimicrobial resistance in our medical intensive care unit (micu), we decided to vary our choice of empiric and therapeuti ... | 2000 | 10988092 |
| microbial dynamics in an extractive membrane bioreactor exposed to an alternating sequence of organic compounds. | wastewaters containing organic compounds have been treated using extractive membrane bioreactors (embs). during treatment, a biofilm normally develops on the surface of the membrane, on the biological side. this study investigates the dynamics of biofilm growth in an emb exposed to an alternating sequence of organic compounds. microbial dynamics of both suspended and attached cultures were investigated experimentally in a single-tube extractive membrane bioreactor (stemb), which comprised a cont ... | 2000 | 10992235 |
| is1414, an escherichia coli insertion sequence with a heat-stable enterotoxin gene embedded in a transposase-like gene. | enteroaggregative escherichia coli (eaec) heat-stable enterotoxin 1 (east1) was originally discovered in eaec but has also been associated with enterotoxigenic e. coli (etec). multiple genomic restriction fragments from each of three etec strains of human origin showed homology with an east1 gene probe. a single hybridizing fragment was detected on the plasmid of etec strain 27d that also encodes heat-stable enterotoxin ib and colonization factor antigen i. we isolated and characterized this fra ... | 2000 | 10992475 |
| molecular detection of burkholderia cepacia in toiletry, cosmetic, and pharmaceutical raw materials and finished products. | a polymerase chain reaction (pcr) assay was developed and compared with standard methods for rapid detection of burkholderia cepacia, a major industrial contaminant, in cosmetic and pharmaceutical raw materials and finished products. artificially contaminated samples were incubated for 24 h in trypticase soy broth containing 4% tween 20 and 0.5% soy lecithin. dna was extracted from each sample using a proteinase k-tris-edta-tween 20 treatment at 35 degrees c. the extracted dna was added to ready ... | 2000 | 10995122 |
| protection of tomato seed germination from the inhibitory effect of 2,4,5-trichlorophenoxyacetic acid by inoculation of soil with burkholderia cepacia ac1100. | the effect of 2,4,5-trichlorophenoxy acetic acid (2,4,5-t) on the germination and seedling vigor of different crop seeds was tested. seeds of rice, maize, sorghum, finger millet, and horse gram were comparatively more tolerant to the chemical with no marked effect up to a concentration of 200 mg 2,4,5-t l(-)(1) as tested by the filter paper method. tomato and brinjal (egg plant) were highly susceptible. even at 5 and 10 mg 2,4,5-t l(-)(1), marked reduction in the germination and seedling vigor o ... | 2000 | 10995357 |
| comparison between the antimicrobial susceptibility of burkholderia pseudomallei to trimethoprim-sulfamethoxazole by standard disk diffusion method and by minimal inhibitory concentration determination. | melioidosis, an infection caused by burkholderia pseudomallei, usually occurs in immunocompromised patients and requires prolonged antibiotic therapy. previously, oral trimethoprim-sulfamethoxazole (tm/sm), an inexpensive and effective drug has been used as a maintenance therapy. the susceptibility of b. pseudomallei to tm/sm by the standard disk diffusion method is very low. however, some patients who were treated with tm/sm as a maintenance therapy despite the in vitro resistance showed good c ... | 2000 | 10998837 |
| pseudomonas cepacia induced septic shock after propofol--a case report. | we report a healthy young female who developed septic shock and multiple organ failure soon after receiving a cosmetic surgery for augmentation of breasts under general anesthesia. blood cultures yielded the growth of pseudomonas cepacia. we describe the clinical course and investigate the causes of the septic shock. contamination of propofol, the intravenous anesthetic agent, was suspected. | 2000 | 11000665 |
| degradation of phenol and benzoic acid in a three-phase fluidized-bed reactor. | degradation of phenol and benzoic acid was studied in a fluidized-bed reactor (liquid volume 2.17 l) under nonsterile conditions with special emphasis on maximizing the flow through the reactor and investigating reactor performance at fluctuating feeds. reactor response to substrate pulses was investigated by applying substrate square-wave inputs at a liquid flow of 1.00 l h(-1). a twofold increase of the phenol and benzoic acid feed concentrations for 2.5 h did not lead to accumulation and brea ... | 2000 | 11005921 |
| modeling substrate interactions during the biodegradation of mixtures of toluene and phenol by burkholderia species js150. | the biodegradation kinetics of toluene, phenol, and a mixture of toluene and phenol by burkholderia species js150 was measured and modeled. both of these compounds can serve as the sole source of carbon and energy for this microorganism. the single-substrate biodegradation kinetics was described well using the monod model, with model constants of mu(max,t) = 0.39 h(-1) and k(s,t) = 0.011 mm for growth on toluene and mu(max,p) = 0.309 h(-1) and k(s,p) = 0.0054 mm for growth on phenol. degradation ... | 2000 | 11005925 |
| use of 16s-rrna to investigate microbial population dynamics during biodegradation of toluene and phenol by a binary culture. | interspecies interactions and changes in the rate and extent of biodegradation in mixed culture-mixed substrate studies were investigated. a binary mixed culture of pseudomonas putida f1 and burkholderia sp. js150 degraded toluene, phenol, and their mixture. both toluene and phenol can serve as sole sources of carbon and energy for both p. putida f1 and strain js150. to investigate the population dynamics of this system, a fluorescent in-situ hybridization method was chosen because of its abilit ... | 2000 | 11005926 |
| enzymes leading to the nucleotide sugar precursors for exopolysaccharide synthesis in burkholderia cepacia. | based on the chemical composition of the exopolysaccharide produced by the cystic fibrosis bacterial isolate burkholderia cepacia ist408, we postulated and confirmed, based on the specificity of enzymes detected in crude cell-free extracts, the pathway leading to the presumptive activated sugar precursors: udp-d-glucose, udp-d-galactose, udp-d-glucuronic acid, gdp-d-mannose, and gdp-d-rhamnose. results also suggest that regulation of the expression of the mucoid phenotype in b. cepacia does not ... | 2000 | 11006084 |
| detection and identification of bacterial endosymbionts in arbuscular mycorrhizal fungi belonging to the family gigasporaceae. | intracellular bacteria have been found previously in one isolate of the arbuscular mycorrhizal (am) fungus gigaspora margarita beg 34. in this study, we extended our investigation to 11 fungal isolates obtained from different geographic areas and belonging to six different species of the family gigasporaceae. with the exception of gigaspora rosea, isolates of all of the am species harbored bacteria, and their dna could be pcr amplified with universal bacterial primers. primers specific for the e ... | 2000 | 11010905 |
| a rhamnolipid biosurfactant reduces cadmium toxicity during naphthalene biodegradation. | a model cocontaminated system was developed to determine whether a metal-complexing biosurfactant, rhamnolipid, could reduce metal toxicity to allow enhanced organic biodegradation by a burkholderia sp. isolated from soil. rhamnolipid eliminated cadmium toxicity when added at a 10-fold greater concentration than cadmium (890 microm), reduced toxicity when added at an equimolar concentration (89 microm), and had no effect at a 10-fold smaller concentration (8.9 microm). the mechanism by which rha ... | 2000 | 11010924 |
| a broad-host-range plasmid for isolating mobile genetic elements in gram-negative bacteria. | plasmid pgbg1 was constructed to isolate mobile genetic elements in a wide variety of gram-negative bacteria. the mutation target, carried on a broad-host-range vector, allows positive selection for tetracycline resistance. in tests using several gram-negative bacteria we could detect transposition events of either insertion sequences or transposons. a new insertion sequence (is) element was identified in ralstonia eutropha. | 2000 | 10964631 |
| toluene-degrading bacteria are chemotactic towards the environmental pollutants benzene, toluene, and trichloroethylene. | the bioremediation of polluted groundwater and toxic waste sites requires that bacteria come into close physical contact with pollutants. this can be accomplished by chemotaxis. five motile strains of bacteria that use five different pathways to degrade toluene were tested for their ability to detect and swim towards this pollutant. three of the five strains (pseudomonas putida f1, ralstonia pickettii pko1, and burkholderia cepacia g4) were attracted to toluene. in each case, the response was de ... | 2000 | 10966434 |
| identification and characteristics of a novel burkholderia strain with broad-spectrum antimicrobial activity. | a burkholderia strain isolated from soil is capable of inhibiting the growth of bacteria, plant-pathogenic fungi, pathogenic yeasts, and protozoa. inhibition does not involve cell contact or the presence of living cells, suggesting that at least a substantial portion of the antimicrobial activity is due to the excretion of extracellular compounds. | 2000 | 10966443 |
| clinical features associated with a delayed diagnosis of cystic fibrosis. | the majority of patients with cystic fibrosis (cf) are diagnosed in the first decade of life. in a small number of patients, the diagnosis is not made until later. | 2000 | 10940794 |
| recovery of burkholderia pseudomallei and b. cepacia from drinking water. | samples of drinking water were examined in order to evaluate the occurrence of two gram-negative bacteria: burkholderia pseudomallei and b. cepacia. a total of 85 samples were collected from public and private buildings in the province of bologna (italy). other bacteriological indicators (heterotrophic plate count at 22 and 36 degrees c) were also examined, together with physical and chemical parameters (temperature, ph, residual chlorine, total hardness and chemical oxygen demand (cod)). high l ... | 2000 | 10946840 |
| burkholderia pseudomallei induces cell fusion and actin-associated membrane protrusion: a possible mechanism for cell-to-cell spreading. | burkholderia pseudomallei, a facultative intracellular bacterium, is the causative agent of a broad spectrum of diseases collectively known as melioidosis. its ability to survive inside phagocytic and nonphagocytic cells and to induce multinucleated giant cell (mngc) formation has been demonstrated. this study was designed to assess a possible mechanism(s) leading to this cellular change, using virulent and nonvirulent strains of b. pseudomallei to infect both phagocytic and nonphagocytic cell l ... | 2000 | 10948167 |
| enhancing the enantioselectivity of lipase in transesterification by substrate matching: an enzyme memory based approach. | the substrate matching strategy is described as a new approach for effectively enhancing the lipase enantioselectivity in organic solvent. in the lipase-catalyzed transesterifications of 3a-c, higher enantioselectivities have been achieved using 1a-c, respectively, as the structurally matched acyl donors. | 2000 | 10956545 |
| pharmacokinetic-pharmacodynamic evaluation of ceftazidime continuous infusion vs intermittent bolus injection in septicaemic melioidosis. | experimental studies have suggested that constant intravenous infusion would be preferable to conventional intermittent bolus administration of beta-lactam antibiotics for serious gram-negative infections. severe melioidosis (burkholderia pseudomallei infection) carries a mortality over 40% despite treatment with high dose ceftazidime. the aim of this study was to measure the pharmacokinetic and pharmacodynamic effects of continuous infusion of ceftazidime vs intermittent bolus dosing in septica ... | 2000 | 10930972 |
| clinical and environmental isolates of burkholderia cepacia exhibit differential cytotoxicity towards macrophages and mast cells. | burkholderia cepacia is an emerging opportunistic pathogen that causes fatal infections in patients suffering from cystic fibrosis (cf) and chronic granulomatous disease. various environmental isolates of b. cepacia are, however, capable of degrading environmental pollutants, such as trichloroethylene, 2,4,5-trichlorophenoxyacetic acid (2,4,5-t), etc., and are also highly effective in controlling plant diseases caused by nematodes and fungi. such strains have therefore been proposed for environm ... | 2000 | 10931297 |
| the novel insertion sequences is1417, is1418, and is1419 from burkholderia glumae and their strain distribution. | three insertion sequences, is1417, is1418, and is1419, were isolated from burkholderia glumae (formerly pseudomonas glumae), a gram-negative rice pathogenic bacterium, on the basis of their abilities to activate the expression of the neo gene of the entrap vector pshi1063. the 1335-bp is1417 element with 17-bp imperfect terminal inverted repeats was found to be flanked by 5-bp direct repeats of the vector sequence. is1418 is 865 bp in length and carries 15-bp inverted repeats with a target dupli ... | 2000 | 10873526 |
| an outbreak of burkholderia cepacia bacteremia in hemodialysis patients: an epidemiologic and molecular study. | the risk of blood stream infections increases in patients undergoing chronic hemodialysis. outbreaks of infection are usually caused by contamination of the water supply, water treatment, distribution system, or dialyzer reprocessing. we report an outbreak of subclavian catheter-related burkholderia cepacia bacteremia in nine patients undergoing hemodialysis. using randomly amplified polymorphic dna (rapd) analysis, the bacterial isolates were clonally identical to burkholderia cepacia isolated ... | 2000 | 10873892 |
| [mobilization of a cryptic plasmid from the melioidosis pathogen in heterologous species of microorganisms]. | cryptic plasmids with different molecular weights have been detected in b. pseudomallei strains. mobilization of b. pseudomallei plasmid dna in heterologous b. mallei species was performed by the conjugate plasmid rp1::tn10. possibility of detecting phenotypical characteristics of plasmids and behavior of b. pseudomallei non-chromosomal replicons in b. mallei have been determined. | 2000 | 10876767 |
| infection control in cystic fibrosis: methicillin-resistant staphylococcus aureus, pseudomonas aeruginosa and the burkholderia cepacia complex. | 2000 | 10911818 | |
| more on the relationship between cystic fibrosis and venous thrombosis. | 2000 | 10914947 | |
| induction of stress shock proteins dnak and groel by phenoxyherbicide 2,4-d in burkholderia sp. yk-2 isolated from rice field. | the purpose of this work was to investigate the induction of stress shock proteins in burkholderia sp. yk-2 in response to the phenoxyherbicide 2,4-dichlorophenoxyacetic acid (2,4-d). the stress shock proteins, which contribute to the resistance of the cytotoxic effect of 2,4-d, were induced at different 2,4-d concentrations in exponentially growing cultures of burkholderia sp. yk-2. this response involved the induction of a 43-kda dnak and 41-kda groel proteins, characterized by sds-page and we ... | 2000 | 10919396 |
| identification of the dimerization domain of dehalogenase iva of burkholderia cepacia mba4. | haloacid dehalogenases are enzymes that catalyze the hydrolytic removal of halogens from haloalkanoic acids. dehalogenase iva (dehiva) from burkholderia cepacia mba4 and dehalogenase ci (dehci) from pseudomonas sp. strain cbs3 exhibit 68% identity. despite their similarity dehiva is a dimeric enzyme while dehci is a monomer. in this work, we describe the identification of the domain that confers the dimerization function of dehiva. recombinant dna molecules were constructed by fusion of the resp ... | 2000 | 10919767 |
| comparison of 2,4-dichlorophenoxyacetic acid degradation and plasmid transfer in soil resulting from bioaugmentation with two different pjp4 donors. | a pilot field study was conducted to assess the impact of bioaugmentation with two plasmid pjp4-bearing microorganisms: the natural host, ralstonia eutropha jmp134, and a laboratory-generated strain amenable to donor counterselection, escherichia coli d11. the r. eutropha strain contained chromosomal genes necessary for mineralization of 2,4-dichlorophenoxyacetic acid (2,4-d), while the e. coli strain did not. the soil system was contaminated with 2,4-d alone or was cocontaminated with 2,4-d and ... | 2000 | 10919798 |
| identification of members of the burkholderia cepacia complex by species-specific pcr. | definitive identification of the species in the burkholderia cepacia complex by routine clinical microbiology methods is difficult. phenotypic tests to identify b. multivorans and b. vietnamiensis have been established; more recent work indicates b. stabilis may also be identified by growth characteristics and biochemical tests. however, attempts to identify genomovars i and iii have, thus far, proved unsuccessful. previously, we demonstrated the utility of two primer pairs, directed to the rrna ... | 2000 | 10921959 |
| utility of commercial systems for identification of burkholderia cepacia complex from cystic fibrosis sputum culture. | performances of several commercial test systems were reviewed to determine their relative levels of accuracy in identifying burkholderia cepacia complex isolates recovered from cystic fibrosis sputum culture. positive predictive values ranged from 71 to 98%; negative predictive values ranged from 50 to 82%. all systems misidentified b. cepacia complex. the species most frequently misidentified as b. cepacia was burkholderia gladioli. these data support the results of previous studies that recomm ... | 2000 | 10921992 |
| laboratory-acquired human glanders--maryland, may 2000. | on may 5, 2000, the baltimore city health department was notified by hospital infection-control staff of a serious systemic febrile illness in a microbiologist whose research at the u.s. army medical research institute of infectious diseases (usamriid) involved several pathogenic bacteria, including burkholderia mallei, the causative agent of glanders. this report summarizes the first human case of glanders in the united states since 1945, and emphasizes the importance of considering occupationa ... | 2000 | 10923853 |
| kinetics of biodegradation of p-nitrophenol by different bacteria. | three bacterial species, i.e., ralstonia sp. sj98, arthrobacter protophormiae rkj100, and burkholderia cepacia rkj200, have been examined for their efficiency and kinetics behavior toward pnp degradation. all the three bacteria utilized pnp as the sole source of carbon, nitrogen, and energy. the rates of radiolabeled [u-(14)c]pnp degradation by all the bacteria were higher in the nitrogen-free medium compared to the medium with nitrogen. the apparent k(m) values of pnp degradation by sj98, rkj10 ... | 2000 | 10924328 |
| isolations of enteric pathogens from synanthropic flies trapped in downtown kuala lumpur. | four species of synanthropic flies were trapped in downtown kuala lumpur: chrysomya megacephala, chrysomya rufifacies, musca domestica, and musca sorbens. burkholderia pseudomallei, the organism causing melioidosis, was the dominant bacteria isolated from chrysomya megacephala. klebsiella oxytoca, commonly associated with nosocomial infections, was commonly isolated from chrysomya megacephala, musca domestica, and musca sorbens. aeromonas hydrophila, the bacteria causing gastroenteritis, was pre ... | 2000 | 10925800 |
| [the role of capsule formation in burkholderia mallei for its persistence in vivo]. | when studied in vivo (in guinea pigs) with the use of electron microscopy, b. mallei (strains c-5, 10230) were found to form a capsule. in the subacute course of infection, the encapsulated forms of b. mallei parasitized mainly in the cells of the system of mononuclear phagocytes in the liver, the spleen and the lungs. the capsule formed by b. mallei was shown to be one of the factors facilitating its persistence in the body. | 2000 | 10925882 |
| preferential adherence of cable-piliated burkholderia cepacia to respiratory epithelia of cf knockout mice and human cystic fibrosis lung explants. | the burkholderia cepacia complex consists of at least five well-documented bacterial genomovars, each of which has been isolated from the sputum of different patients with cystic fibrosis (cf). although the world-wide prevalence of this opportunist pathogen in cf patients is low (1-3%), 'epidemic' clusters occur in geographically isolated regions. prevalence in some of these clusters is as high as 30-40%. the majority of cf b. cepacia isolates belong to genomovar iii, but the relationship betwee ... | 2000 | 11023184 |
| ralstonia pickettii colonization of patients in an obstetric ward caused by a contaminated irrigation system. | 2000 | 11023730 | |
| molecular cloning and characterization of a chitosanase from the chitosanolytic bacterium burkholderia gladioli strain chb101. | a chitosanase was purified from the culture fluid of the chitino- and chitosanolytic bacterium burkholderia gladioli strain chb101. the purified enzyme (chitosanase a) had a molecular mass of 28 kda, and catalyzed the endo-type cleavage of chitosans having a low degree of acetylation (0-30%). the enzyme hydrolyzed glucosamine oligomers larger than a pentamer, but did not exhibit any activity toward n-acetylglucosamine oligomers and colloidal chitin. the gene coding for chitosanase a (csna) was i ... | 2000 | 11030572 |
| phospholipid compositional changes of five pseudomonad archetypes grown with and without toluene. | bacterial physiological responses to toluene exposure were investigated in five reference pseudomonad strains that express different toluene degradation pathways: pseudomonas putida mt-2, pseudomonas putida f1, burkholderia cepacia g4, burkholderia pickettii pko1, and pseudomonas mendocina kr1. the intact phospholipids of these archetypes, grown with and without toluene, were characterized using liquid chromatography/electrospray ionization/mass spectrometry. all strains showed significant chang ... | 2000 | 11030576 |
| sequencing and characterization of a novel serine metalloprotease from burkholderia pseudomallei. | burkholderia pseudomallei, a gram-negative bacterium is found in the soil and water, mainly in southeast asia and northern australia. it is responsible for melioidosis in human and animals. the bacteria produce several potential virulent factors such as extracellular protease, hemolysin, lipase and lecithinase. the isolation of virulence genes and the study of their functions will contribute to our understanding of bacterial pathogenesis. previous studies have implicated protease as a contributi ... | 2000 | 11040430 |
| rhizosphere competitiveness of trichloroethylene-degrading, poplar-colonizing recombinant bacteria. | indigenous bacteria from poplar tree (populus canadensis var. eugenei 'imperial carolina') and southern california shrub rhizospheres, as well as two tree-colonizing rhizobium strains (atcc 10320 and atcc 35645), were engineered to express constitutively and stably toluene o-monooxygenase (tom) from burkholderia cepacia g4 by integrating the tom locus into the chromosome. the poplar and rhizobium recombinant bacteria degraded trichloroethylene at a rate of 0.8 to 2.1 nmol/min/mg of protein and w ... | 2000 | 11055909 |
| case reports of death during pregnancy in patients with cystic fibrosis--three out of four patients were colonized with burkholderia cepacia. | 2000 | 11059957 | |
| aerobic degradation of mixtures of chlorinated aliphatics by cloned toluene-o-xylene monooxygenase and toluene o-monooxygenase in resting cells. | recombinant strains of escherichia coli constitutively expressing toluene-o-xylene monooxygenase (tomo) of pseudomonas stutzeri ox1 and toluene o-monooxygenase (tom) of burkholderia cepacia g4 were investigated for their ability to oxidize trichloroethylene (tce), 1,1-dichloroethylene (1,1-dce), cis-1,2-dichloroethylene (cis-dce), trans-1,2-dichloroethylene (trans-dce), vinyl chloride (vc), and chloroform (cf), individually as well as in various mixtures. tomo oxidized all of these individual co ... | 2000 | 11064339 |
| the use of protocatechuate dioxygenase for maintaining anaerobic conditions in biochemical experiments. | the study of redox-active systems often requires the maintenance of anaerobic conditions. the glucose oxidase system has often been used to maintain anaerobic conditions, but it has some drawbacks, such as the production of h(2)o(2) and limitations on stability. protocatechuate dioxygenase from burkholderia cepacia and the substrate, protocatechuate, constitute an alternate effective oxygen-scrubbing system that can be used in a wide variety of biochemical experiments. we have shown its suitabil ... | 2000 | 11067739 |
| cloning and analysis of genomic differences unique to burkholderia pseudomallei by comparison with b. thailandensis. | melioidosis is an infectious disease caused by burkholderia pseudomallei. genomic subtractive hybridisation was performed with the closely related avirulent species b. thailandensis to identify virulence genes of b. pseudomallei. the subtractive hybridisation products were highly specific for b. pseudomallei. sequence analysis revealed a number of putative virulence factors, as well as apparently novel sequences of unknown function. the subtracted library contained dna regions of relatively low ... | 2000 | 11073153 |
| genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in sphingomonas paucimobilis syk-6. | protocatechuate (pca) is the key intermediate metabolite in the lignin degradation pathway of sphingomonas paucimobilis syk-6 and is metabolized to pyruvate and oxaloacetate via the pca 4,5-cleavage pathway. we characterized the 4-carboxy-2-hydroxymuconate-6-semialdehyde (chms) dehydrogenase gene (ligc). chms is the 4,5-cleavage product of pca and is converted into 2-pyrone-4,6-dicarboxylate (pdc) by ligc. we found that ligc was located 295 bp downstream of ligb, which encodes the large subunit ... | 2000 | 11073908 |
| bias caused by using different isolation media for assessing the genetic diversity of a natural microbial population. | the influence of isolation medium on the biodiversity of burkholderia cepacia strains recovered from the rhizosphere of zea mays was evaluated by comparing the genetic diversity of isolates obtained by plating serial dilutions of root macerates on the two selective media tb-t and pcat. from each medium, 50 randomly chosen colonies were isolated. on the basis of the restriction patterns of dna coding for 16s rrna (16s rdna) amplified by means of pcr (ardra), all strains isolated from tb-t medium ... | 2000 | 11080375 |
| aquifer protist response and the potential for tce bioremediation with burkholderia cepacia g4 pr1. | bacterivorous protists have been recovered from pristine and contaminated aquifer environments, but the ecological role of these organisms in bioremediation strategies has not been well defined. burkholderia cepacia g4 pr1 constitutively expresses a toluene ortho-monooxygenase (tom) due to a secondary transposition of a tn5 transposable element in a trichloroethylene (tce) degradative plasmid (tom). groundwater and sediment from a potential site for a tce bioremediation field demonstration were ... | 2000 | 11080377 |
| melioidosis research in china. | research on melioidosis and its pathogen has been ongoing in china for more than two decades. it has been demonstrated that the natural foci are located predominantly in hainan, guangdong and guangxi province, where there is a good correlation between soil isolation and the serum prevalence of antibodies to burkholderia pseudomallei. the cases of melioidosis reported up to now are concentrated in the hainan and zhanjiang peninsula. investigations on serotype, virulence, ecology, antibiotic susce ... | 2000 | 11080506 |
| genotype analysis of burkholderia pseudomallei using randomly amplified polymorphic dna (rapd): indicative of genetic differences amongst environmental and clinical isolates. | burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease common in the tropics. melioidosis is most prevalent in the northeastern part of thailand. the diseases has diverse clinical manifestations ranging from mild localized to fatal septicemic forms. the bacterial genetic factors contributing to the severity of melioidosis have not been completely identified. we have developed a genotyping method based upon randomly amplified polymorphic dna (rapd) analysis. eighte ... | 2000 | 11080514 |
| identification of a siderophore receptor required for ferric ornibactin uptake in burkholderia cepacia. | ornibactins are linear hydroxamate siderophores produced by burkholderia cepacia with peptide structures similar to that of pyoverdines produced by the fluorescent pseudomonads. the gene encoding the outer membrane receptor (orba) was identified, sequenced, and demonstrated to have significant homology with hydroxamate receptors produced by other organisms. the orba precursor was predicted to be a protein with a molecular mass of 81 kda. an orba mutant was constructed and demonstrated to be unab ... | 2000 | 11083764 |
| pgstp: an ivet-compatible promoter probe vector conferring resistance to trimethoprim. | 2000 | 11084853 | |
| computational studies of essential dynamics of pseudomonas cepacia lipase. | in order to investigate the interfacial activation of a lipase from pseudomonas cepacia (pcl), molecular dynamics (md) simulations and essential dynamics (ed) analysis were performed in different solvent environments: vacuum and explicit water solvents. starting from the active (open) structure of pcl, the essential dynamics analysis of the simulations revealed large correlated motions, which may be responsible for the activation of the enzyme. fluctuations in the u1 (active-site lid) and u2 dom ... | 2000 | 11089650 |
| outbreak of nosocomial burkholderia cepacia infection and colonization associated with intrinsically contaminated mouthwash. | from august 1996 through june 1998, 69 ventilated, intensive care unit patients at two arizona hospitals had nosocomial respiratory tract cultures positive for burkholderia cepacia. intrinsically contaminated alcohol-free mouthwash was identified by pulsed-field gel electrophoresis as the source of the outbreak. | 2000 | 11089663 |
| 16s rdna analysis for characterization of denitrifying bacteria isolated from three agricultural soils. | bacteria capable of denitrification are spread among phylogenetically diverse groups. in the present investigation, molecular methods (amplified ribosomal dna restriction analysis (ardra) and partial 16s rdna gene sequencing) were used to determine the genetic diversity of culturable denitrifying soil bacteria. the purpose of this work was to study the microbial density and diversity of denitrifying communities isolated from two luvisols and a rendosol. the denitrifying bacterial density was sig ... | 2000 | 11102689 |
| [evaluation of bacterial contamination in disinfectants for domestic use]. | to evaluate disinfectants for domestic use for the presence of bacteria, identify them, and determine their tolerance level to benzalkonium chloride. | 2000 | 11105107 |
| mouse model of sublethal and lethal intraperitoneal glanders (burkholderia mallei). | sixty male balb/c mice were inoculated intraperitoneally with either a sublethal or a lethal dose of burkholderia mallei china 7 strain, then killed at multiple time points postinoculation. histopathologic changes were qualitatively similar in both groups and consisted of pyogranulomatous inflammation. in sublethal study mice, changes were first seen at 6 hours in mediastinal lymph nodes, then in spleen, liver, peripheral lymph nodes, and bone marrow at day 3. these changes generally reached max ... | 2000 | 11105952 |
| characterization of burkholderia pseudomallei isolated in thailand and malaysia. | a total of 35 burkholderia pseudomallei isolates from thailand (16 clinical and eight soil isolates) and malaysia (seven animal, two isolate each from clinical and soil) were investigated by their antimicrobial resistance, plasmid profiles and were typed by randomly amplified polymorphic dna analysis. all isolates were found to be resistant to six or more of the 12 antimicrobial agents tested. only two small plasmids of 1.8 and 2.4 megadalton were detected in two clinical isolates from thailand. ... | 2000 | 11109011 |
| dna-based diagnostic approaches for identification of burkholderia cepacia complex, burkholderia vietnamiensis, burkholderia multivorans, burkholderia stabilis, and burkholderia cepacia genomovars i and iii. | bacteria of the burkholderia cepacia complex consist of five discrete genomic species, including genomovars i and iii and three new species: burkholderia multivorans (formerly genomovar ii), burkholderia stabilis (formerly genomovar iv), and burkholderia vietnamiensis (formerly genomovar v). strains of all five genomovars are capable of causing opportunistic human infection, and microbiological identification of these closely related species is difficult. the 16s rrna gene (16s rdna) and reca ge ... | 2000 | 10970351 |
| characterization and mutagenesis of fur gene from burkholderia pseudomallei. | a homolog of the ferric uptake regulator gene (fur) was isolated from burkholderia pseudomallei (bp) by a reverse genetic technique. sequencing of a 2.2kb dna fragment revealed an open reading frame with extensive homology to bacterial fur proteins. the cloned gene encodes a 16kda protein that cross-reacts with a polyclonal anti-escherichia coli fur serum. the transcription start site was determined by the primer extension technique. expression analysis of fur showed no increased fur mrna levels ... | 2000 | 10974543 |
| melioidosis: acute and chronic disease, relapse and re-activation. | in melioidosis-endemic regions the importance of re-activation of burkholderia pseudomallei from latent foci remains unclear. this topic was assessed in a 10-year prospective study (1989-99) of melioidosis in the tropical north of the northern territory of australia, together with other aspects of the nature of melioidosis. incubation period from defined inoculating events was previously ascertained as 1-21 (mean 9) days. of 252 total cases 244 (97%) were considered to be from recent acquisition ... | 2000 | 10975006 |
| studies on polyhydroxyalkanoate (pha) accumulation in a pha synthase i-negative mutant of burkholderia cepacia generated by homogenotization. | in the genome of burkholderia cepacia strain ipt64, which accumulates a blend of the two homopolyesters poly(3-hydroxybutyrate), poly(3hb), and poly(3-hydroxy-4-pentenoic acid), poly(3h4pe), from sucrose or gluconate as single carbon source, the polyhydroxyalkanoate (pha) synthase structural gene was disrupted by the insertion of a chloramphenicol-resistant gene cassette (phac1::cm). the suicide vector psup202 harboring phac1::cm was transferred to b. cepacia by conjugation. the inactivated gene ... | 2000 | 11094298 |
| zoospore homing and infection events: effects of the biocontrol bacterium burkholderia cepacia ammdr1 on two oomycete pathogens of pea (pisum sativum l.). | burkholderia cepacia ammdr1 is a biocontrol agent that protects pea and sweet corn seeds from pythium damping-off in field experiments. the goal of this work was to understand the effect of b. cepacia ammdr1 on pythium aphanidermatum and aphanomyces euteiches zoospore homing events and on infection of pea seeds or roots. in vitro, b. cepacia ammdr1 caused zoospore lysis, prevented cyst germination, and inhibited germ tube growth of both oomycetes. b. cepacia ammdr1 also reduced the attractivenes ... | 2000 | 11097889 |
| bacterial lipases from pseudomonas: regulation of gene expression and mechanisms of secretion. | lipases from pseudomonas bacteria are widely used for a variety of biotechnological applications. overexpression in heterologous hosts like escherichia coli failed to produce enzymatically active lipase prompting to study the molecular mechanisms underlying the regulation of lipase gene expression and secretion. the prototype lipase from p. aeruginosa is encoded in a bicistronic operon which is transcribed from two different promotors, one of which depends on the alternative sigma factor rpon (s ... | 2000 | 11099799 |
| unusual respiratory bacterial flora in cystic fibrosis: microbiologic and clinical features. | pulmonary infections continue to be a significant source of morbidity and mortality among patients with cystic fibrosis. although our understanding of the pathogenesis and clinical consequences of pulmonary infections with pseudomonas aeruginosa has increased greatly in recent years, very little is known about potentially emerging pathogens such as burkholderia cepacia complex, stenotrophomonas maltophilia, alcaligenes xylosoxidans, and methicillin-resistant staphylococcus aureus. in this review ... | 2000 | 11100967 |
| kinetic resolution of racemic alpha-methyl-beta-propiothiolactone by lipase-catalyzed hydrolysis. | kinetic resolution of racemic alpha-methyl-beta-propiothiolactone (rac-mptl) using lipases in organic solvent was studied. the lipase from pseudomonas cepacia (pcl) showed the highest (s)-enantioselectivity (e > 100), and cyclohexane containing 1% (v/v) buffer was identified as the best reaction medium for maintaining high enantioselectivity as well as high reaction rate. while the substrate inhibition was not observed up to 300 mm rac-mptl, severe product inhibition was observed even at 50 mm r ... | 2000 | 11101323 |
| identification and detection of stenotrophomonas maltophilia by rrna-directed pcr. | stenotrophomonas maltophilia has recently emerged as an important nosocomial pathogen in immunocompromised patients, in transplant recipients, and in persons with cystic fibrosis (cf). while this organism is nonpathogenic in healthy individuals, it is increasingly associated with morbidity and mortality in susceptible populations. recent studies have indicated that for approximately 10% of cf patients with moderate lung disease, s. maltophilia can be cultured from respiratory tract secretions. i ... | 2000 | 11101555 |
| fulminant septic melioidosis after a vacation in thailand. | severe infection with burkholderia pseudomallei (formerly pseudomonas pseudomallei), the bacterium causing melioidosis, is a common cause of acquired septicaemia in south-east asia and northern australia. a few cases of infected travellers returning to european countries have been reported. melioidosis is a tropical disease, the clinical presentation ranging from asymptomatic infection to fulminant sepsis. predisposing conditions such as impaired cellular immunity, preexisting renal failure or d ... | 2000 | 11244616 |
| bacterial community changes and enrichment of burkholderia-like bacteria induced by chlorinated benzoates in a peat-forest soil-microcosm. | bacterial community shifts in a peat-forest soil spiked with 3-chlorobenzoate (3cba) or 2,5-dichlorobenzoate (2,5dcb) were monitored by pcr-amplification of the v6 to v8 regions of the 16s rrna and rdna, followed by separation of the amplicons by temperature gradient gel electrophoresis. 3cba disappeared to non-detectable levels after 15 days by a biologically mediated process, while 2,5dcb remained at the initial concentration values. the experiments were conducted under microcosms systems. add ... | 2000 | 11249031 |
| different portions of the maize root system host burkholderia cepacia populations with different degrees of genetic polymorphism. | in order to acquire a better understanding of the spatial and temporal variations of genetic diversity of burkholderia cepacia populations in the rhizosphere of zea mays, 161 strains were isolated from three portions of the maize root system at different soil depths and at three distinct plant growth stages. the genetic diversity among b. cepacia isolates was analysed by means of the random amplified polymorphic dna (rapd) technique. a number of diversity indices (richness, shannon diversity, ev ... | 2000 | 11243257 |
| role of commensal relationships on the spatial structure of a surface-attached microbial consortium. | a flow cell-grown model consortium consisting of two organisms, burkholderia sp. lb400 and pseudomonas sp. b13(fr1), was studied. these bacteria have the potential to interact metabolically because pseudomonas sp. b13(fr1) can metabolize chlorobenzoate produced by burkholderia sp. lb400 when grown on chlorobiphenyl. the expected metabolic interactions in the consortium were demonstrated by high performance liquid chromatography (hplc) analysis. the spatial structure of the consortium was studied ... | 2000 | 11243263 |
| a cluster exposed: structure of the rieske ferredoxin from biphenyl dioxygenase and the redox properties of rieske fe-s proteins. | ring-hydroxylating dioxygenases are multicomponent systems that initiate biodegradation of aromatic compounds. many dioxygenase systems include rieske-type ferredoxins with amino acid sequences and redox properties remarkably different from the rieske proteins of proton-translocating respiratory and photosynthetic complexes. in the latter, the [fe2s2] clusters lie near the protein surface, operate at potentials above +300 mv at ph 7, and express ph- and ionic strength-dependent redox behavior. t ... | 2000 | 11188691 |
| [pathogenicity of burkholderia pseudomallei: extracellular and surface antigen functions]. | the data of literature and the results of investigations carried out by the authors on the analysis of b. pseudomallei pathogenicity factors. they include mucoid, endotoxin, lecithinase, proteases, hemolysins, etc. in addition to fimbriae and pili, the adhesive properties of b. pseudomallei are supposed to be associated with surface capsule-like structures whose composition includes ag8. the characterization of exoproteases, hemolysins and lethal toxins is given. the virulence and immunogenicity ... | 2000 | 11210655 |
| transient asceptic arthritis of knees in a patient with melioidosis. | osteomyelitis and septic arthritis are known manifestations of melioidosis. whether reactive arthritis can occur as a sequele to b. pseudomallei infection, is yet to be seen. as more and more cases of melioidosis will be reported in future, this aspect has to be kept in mind. | 2000 | 11214516 |
| preferential occurrence of diazotrophic endophytes, azoarcus spp., in wild rice species and land races of oryza sativa in comparison with modern races. | several diazotrophic species of azoarcus spp. occur as endophytes in the pioneer plant kallar grass. the purpose of this study was to screen asian wild rice and cultivated oryza sativa varieties for natural association with these endophytes. populations of culturable diazotrophs in surface-sterilized roots were characterized by 16s rdna sequence analysis, and azoarcus species were identified by genomic fingerprints. a. indigens and azoarcus sp. group c were detected only rarely, whereas azoarcus ... | 2000 | 11220300 |
| comparison of the polymerase chain reaction and serologic tests for diagnosis of septicemic melioidosis. | for diagnosis of melioidosis, we compared polymerase chain reaction (pcr)-based dna detection and three serologic methods with the culture method currently used as gold standard. the diagnostic values of the serologic methods were evaluated in 130 patients. all these patients resided in an endemic area. an enzyme-linked immunosorbent assay (elisa) gave slightly higher specificity (86.2%) than a dot immunoassay (dot) (85.3%), but was superior to an indirect hemagglutination assay (iha) (79.8%). t ... | 2000 | 11388506 |
| antigenic heterogeneity of lipopolysaccharide among burkholderia pseudomallei clinical isolates. | burkholderia pseudomallei (bp) causes melioidosis, a potentially fatal human infection in the tropics. clinical isolates from different geographical locations have similar morphological and biochemical characteristics. although bp has been reported to possess 2 types of lipopolysaccharide (lps) differing in the chemical structure of their o-polysaccharide (o-ps) component, earlier report demonstrated that the clinical strains exhibited identical lps moieties. recently, we reported antigenic simi ... | 2000 | 11414445 |