Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| use of the bactec myco/f lytic medium for detection of brucella melitensis bacteremia. | in a prospective volume-controlled study, the sensitivity of the bactec myco/f lytic medium was similar to that of the peds plus/f and plus aerobic/f vials for detecting brucella melitensis bacteremia, but the time to detection with the myco/f lytic medium (101.4 +/- 46.7 h) was significantly longer than that with the combined comparator media (65.5 +/- 18.9 h, p = 0.004). | 2004 | 15131190 |
| pathologic changes associated with brucellosis experimentally induced by aerosol exposure in rhesus macaques (macaca mulatta). | to develop an aerosol exposure method for induction of brucellosis in rhesus macaques (macaca mulatta). | 2004 | 15141886 |
| antibody response and antigen-specific gamma-interferon profiles of vaccinated and unvaccinated pregnant sheep experimentally infected with brucella melitensis. | it is well known that the immune response in sheep against brucella melitensis is subject to individual variation, depending on diverse factors. it bears asking whether these factors (e.g. clinical disease, active infection, state of previous immunity), when affecting a group, can cause variation in the performance of different diagnostic tests. to clarify some of the circumstances in which this immune response can vary, we examine the immune-response profile of sheep protected against the clini ... | 2004 | 15145500 |
| development of a western blotting assay to discriminate brucella spp. and yersinia enterocolitica o:9 infections in sheep. | outer membrane proteins (omps) of rev-1 strain of brucella melitensis were used in a western blotting assay for the serological diagnosis of brucellosis in ovine sera. fifty-four sheep sera were tested and divided into the following groups: group a) n. 9 samples from one sheep that had been experimentally infected with y. enterocolitica o:9; group b) n. 10 samples collected from sheep infected with brucella melitensis and 1 sample from a sheep vaccinated with the rev 1 strain; group c) n. 10 sam ... | 2004 | 15164626 |
| the louse-borne human pathogen bartonella quintana is a genomic derivative of the zoonotic agent bartonella henselae. | we present the complete genomes of two human pathogens, bartonella quintana (1,581,384 bp) and bartonella henselae (1,931,047 bp). the two pathogens maintain several similarities in being transmitted by insect vectors, using mammalian reservoirs, infecting similar cell types (endothelial cells and erythrocytes) and causing vasculoproliferative changes in immunocompromised hosts. a primary difference between the two pathogens is their reservoir ecology. whereas b. quintana is a specialist, using ... | 2004 | 15210978 |
| oral vaccination with brucella melitensis wr201 protects mice against intranasal challenge with virulent brucella melitensis 16m. | human brucellosis can be acquired from infected animal tissues by ingestion, inhalation, or contamination of conjunctiva or traumatized skin by infected animal products. in addition, brucella is recognized as a biowarfare threat agent. although a vaccine to protect humans from natural or deliberate infection could be useful, vaccines presently used in animals are unsuitable for human use. we tested orally administered live, attenuated, purine auxotrophic b. melitensis wr201 bacteria for their ab ... | 2004 | 15213148 |
| survey of the seroprevalence of brucellosis in ruminants in kosovo. | a cross-sectional survey of the seroprevalence of brucellosis in sheep, goats and cattle in kosovo was made in january 2001. a total of 12,000 serum samples, from 7941 cattle, 3548 sheep and 511 goats, were screened using the rose bengal test. doubtful and positive results were further tested with competitive and indirect elisas. the overall serological prevalences derived from the samples positive to all three tests, were 6.26 per cent (95 per cent confidence intervals [ci] 5.5 to 7.1 per cent) ... | 2004 | 15224594 |
| prevention of laboratory-acquired brucellosis. | brucellosis is one of the most common laboratory-acquired infections, mostly because aerosolization is a mechanism of transmission in this setting. we report an exposure to brucella melitensis that occurred in a large microbiology laboratory and describe the strategy chosen for antibiotic prophylaxis and serological follow-up of exposed workers. | 2004 | 15227634 |
| childhood brucellosis--a microbiological, epidemiological and clinical study. | a total of 5726 blood specimens (from children aged 14 years and younger) were studied for the serological evidence of brucellosis. ninety-three (1.6 per cent) showed diagnostic agglutinin titres with a geometric mean titre of 403 (sd +/- 547). forty-three (59.7 per cent) blood specimens yielded the growth of brucella melitensis. thirty-nine patients (41.93 per cent) were shepherds, who constituted the major occupational group affected in the present series. more than 60 per cent of the patients ... | 2004 | 15233191 |
| development and evaluation as vaccines in mice of brucella melitensis rev.1 single and double deletion mutants of the bp26 and omp31 genes coding for antigens of diagnostic significance in ovine brucellosis. | the live attenuated brucella melitensis rev.1 strain is considered the best vaccine available for the prophylaxis of brucellosis in sheep caused by either b. melitensis or brucella ovis. however, its application stimulates antibody responses in vaccinated animals indistinguishable by the current conventional serological tests from those observed in infected animals. the periplasmic protein bp26 and the outer membrane protein (omp) omp31 are immunodominant antigens in the serological responses of ... | 2004 | 15246618 |
| popliteal artery pseudoaneurysm: a rare complication of brucellosis. | brucellosis is still a public health problem, particularly in developing countries. after the primary infection subsides, a chronic stage characterized by nonspecific manifestations can develop, during which it may not be possible to isolate brucella organisms and agglutination test titers may or may not be high. we present the case of a 49-year-old man who had only nonspecific symptoms and a 2-month history of a pulsatile painful swelling in his right popliteal region. he had no history of trau ... | 2004 | 14705176 |
| brucellar spine infection--four case reports. | brucellosis can be difficult to diagnose because of the nonspecific and variable clinical picture. this systemic disease is still an important public health problem in the mediterranean basin. these four cases of brucellar spine infection originated from rural areas around aydin, turkey. the systemic complaints of fever, profuse sweating, malaise, polyarthromyalgia, and weight loss indicated the final diagnosis of brucellosis and presumptive diagnoses were made based on agglutination testing for ... | 2004 | 15633472 |
| a multiplexed pcr-coupled liquid bead array for the simultaneous detection of four biothreat agents. | we have developed a 10-plexed pcr assay coupled to a 12-plexed liquid bead array to rapidly screen environmental samples for b. anthracis, y. pestis, f. tularensis, and b. melitensis. highly validated species-specific primer sets were used to simultaneously amplify multiple diagnostic regions unique to each individual pathogen. resolution of the mix of amplified products was achieved by pcr product hybridization to corresponding probe sequences, attached to unique sets of fluorescent beads. the ... | 2004 | 15680215 |
| community-acquired bacteraemia in a rural area: predominant bacterial species and antibiotic resistance. | the invasion of the bloodstream represents one of the most important sequelae of infection. this study was conducted over an 18-month period to determine the predominant bacterial agents of a community-acquired bacteraemia seen at health centres in a rural area of jordan, and their antibiotic susceptibilities. blood samples were collected and cultured from 215 patients who presented with fever and presumed diagnosis of a bacteraemia. isolates were identified and tested for antibiotic susceptibil ... | 2004 | 15358829 |
| unusual clinical presentations of brucellosis. | the purpose of this paper is to draw attention to atypical presentations of brucellosis. a prospective study identified 240 consecutive patients with brucellosis admitted to our department between december 1999 and july 2002. from these cases we present 11 patients with unusual clinical presentations. neurobrucellosis, peritonitis, pericarditis, pancytopenia, and uveitis were diagnosed in 2 patients each and 1 presented with epididymo-orchitis. | 2004 | 15370660 |
| dna polymorphism in the omp25/omp31 family of brucella spp.: identification of a 1.7-kb inversion in brucella cetaceae and of a 15.1-kb genomic island, absent from brucella ovis, related to the synthesis of smooth lipopolysaccharide. | five genes homologous to the well-known omp25 and omp31 genes, that code for two major brucella spp. outer membrane proteins (omps), have been detected in the genome of brucella melitensis 16m and brucella suis 1330. in this work we have determined the nucleotide sequence of these five genes, named omp31b, omp25b, omp25c, omp25d and omp22, in the six classical brucella species reference strains and in representative strains of the recently proposed species brucella cetaceae and brucella pinniped ... | 2004 | 15374004 |
| isolated intracranial hypertension: a rare presentation of neurobrucellosis. | brucella melitensis infection is endemic in the eastern and south-eastern anatolia regions of turkey. we report an unusual case of brucella meningitis presenting with bilateral papilla stasis, diplopia and absence of other neurological involvement. diagnosis was made by positive culture of brucella spp. with a bactec 9120 system with inoculation of the patient's cerebrospinal fluid (csf). this is the first report of isolation of brucella spp. from csf on a bactec 9120 system for diagnosis of men ... | 2004 | 15374008 |
| release of periplasmic proteins of brucella suis upon acidic shock involves the outer membrane protein omp25. | the survival and replication of brucella in macrophages is initially triggered by a low intraphagosomal ph. in order to identify proteins released by brucella during this early acidification step, we analyzed brucella suis conditioned medium at various ph levels. no significant proteins were released at ph 4.0 in minimal medium or citrate buffer, whereas in acetate buffer, b. suis released a substantial amount of soluble proteins. comparison of 13 n-terminal amino acid sequences determined by ed ... | 2004 | 15385468 |
| the ton system, an abc transporter, and a universally conserved gtpase are involved in iron utilization by brucella melitensis 16m. | brucella spp. are gram-negative intracellular facultative pathogens that are known to produce 2,3-dihydroxybenzoic acid (dhba), a catechol siderophore that is essential for full virulence in the natural host. the mechanism of dhba entry into brucella and other gram-negative bacteria is poorly understood. using mini-tn5kmcat mutagenesis, we created a transposon library of brucella melitensis 16m and isolated 32 mutants with a defect in iron acquisition or assimilation. three of these transposon m ... | 2004 | 15385478 |
| brucellosis in the west bank, palestine. | to identify the risk factors for brucellosis in palestine, to estimate their association with the disease and to recommend appropriate prevention measures. | 2004 | 15573193 |
| indirect elisa for the detection of antibodies to brucella melitensis in sheep milk. | 2004 | 15573789 | |
| genetic bases of the rifampin resistance phenotype in brucella spp. | rifampin is one of the most potent and broad-spectrum antibiotics against bacterial pathogens. its bactericidal activity is due to its ability to bind to the beta subunit of the dna-dependent rna polymerase encoded by the rpob gene. mutations of the rpob gene have been characterized in rifampin-resistant (rif(r)) strains of escherichia coli and mycobacterium tuberculosis. the genetic bases of rif(r) in brucella spp. are still unknown. in the present study, the nucleotide sequences of the rpob ge ... | 2004 | 15583262 |
| the bartonella henselae sucb gene encodes a dihydrolipoamide succinyltransferase protein reactive with sera from patients with cat-scratch disease. | bartonella henselae is a recently recognized pathogenic bacterium associated with cat-scratch disease, bacillary angiomatosis and bacillary peliosis. a recombinant clone expressing an immunoreactive antigen of b. henselae was isolated by screening a genomic dna cosmid library by western blotting with sera pooled from patients positive for b. henselae igg antibodies by indirect immunofluorescence (ifa). the deduced amino acid sequence of the 43.7 kda encoded protein was found to be 76.3 % identic ... | 2004 | 15585501 |
| pancytopenia, a rare hematologic manifestation of brucellosis in children. | the records of 54 children with brucellosis were evaluated retrospectively. among them, eight patients (14.8%) with pancytopenia were identified in a 7-year period between 1996 and 2003. six of the eight patients with pancytopenia had brucella melitensis isolated from blood cultures, and all eight patients had brucella agglutination titers of at least 1:320. agglutination test titers did not correlate with the degree of pancytopenia. fever was the most common manifestation, followed by malaise, ... | 2004 | 15591899 |
| [renal brucelloma: an exceptional pathology]. | to report one case of renal brucelloma, an extraordinarily rare pathology in our environment. | 2004 | 15714853 |
| human and animal brucellosis in jordan between 1996 and 1998: a study. | between 1996 and 1998, a total of 2,494 samples of blood from humans and animals were collected and tested for brucellosis. this total included 1,594 samples of animal blood, collected from 1,050 sheep from 20 flocks, and 544 goats from eight herds. the serum samples were tested using the rose bengal test, the tube agglutination test, the complement fixation test and an enzyme-linked immunosorbent assay. moreover, a complete history was compiled from each flock/herd. the rate of abortions in she ... | 2004 | 15861878 |
| comparison of serological tests for the detection of ovine and caprine antibody to brucella melitensis. | the indirect enzyme-linked immunosorbent assay (ielisa), the competitive enzyme-linked immunosorbent assay (celisa) and the fluorescence polarisation assay (fpa) were evaluated with sera from sheep experimentally infected with brucella melitensis and negative canadian sheep. the sensitivity and specificity of the assays were as follows: ielisa: 91.7% and 97.6%, celisa: 75.0% and 99.8% and fpa: 91.7% and 89.5%. sera from the same experimental population were divided according to serological react ... | 2004 | 15861894 |
| serum neopterin levels in patients with brucellosis. | the aim of this study was to evaluate the importance of neopterin levels in brucellosis and its alterations with treatment. | 2004 | 16291280 |
| [development of immunoreagents for the diagnostics of intestinal yersiniosis by antigen-binding lymphocytes]. | to diagnose intestinal yersiniosis, the detection of antigen-binding lymphocytes (abl) with respect to y. enterocolitica o3, o5 and o9 is proposed. experiments on the immunization of rabbits with y. enterocolitica lps of these serovars revealed that immunoreagents, according to the data of cross antigen-dependent rosette formation and its inhibition, had species specificity (abl of rabbits immunized with y. enterocolitica did not interact with salmonella, shigella and y. pseudotuberculosis antig ... | 2004 | 15636146 |
| brucellar spondylitis. | we carried out a prospective analysis of 86 patients with brucellosis, and 26 (30%) cases were diagnosed as brucellar spondylitis. two patients had cervical involvement, two thoracic, and 21 lumbosacral as seen in mri. four patients had epidural abscess and two had paravertebral abscess. all patients received combined antibiotic therapy for 4 to 12 months. those with cervical involvement underwent surgical treatment because of medullar compression. neither death nor severe sequelae were observed ... | 2004 | 12879273 |
| severe thrombotic microangiopathy associated with brucellosis: successful treatment with plasmapheresis. | brucellosis is a disease that may lead to changes in hematologic parameters such as anemia, neutropenia, and thrombocytopenia; however, thrombotic microangiopathy (tma) is a rare finding. severe tma may be associated with life-threatening hematologic, renal, and neurologic disorders. to prevent this mortality caused by brucellosis, prompt recognition of this complication and prompt therapy are essential. a patient with tma associated with brucella melitensis is presented who initially presented ... | 2005 | 15678281 |
| intravenous gamma globulin is effective as an urgent treatment in brucella-induced severe thrombocytopenic purpura. | severe thrombocytopenia is a rare hematologic manifestation of brucellosis, which can occasionally be associated with bleeding into the skin and from mucosal sites. prompt recognition of this brucellosis complication and aggressive therapy is vital because the mortality rate associated with bleeding into the central nervous system is high. we report a case of a patient infected with brucella melitensis who was admitted with a severe case of thrombocytopenic purpura. the patient responded well to ... | 2005 | 16247741 |
| vaccination with the recombinant brucella outer membrane protein 31 or a derived 27-amino-acid synthetic peptide elicits a cd4+ t helper 1 response that protects against brucella melitensis infection. | the immunogenicity and protective efficacy of the recombinant 31-kda outer membrane protein from brucella melitensis (romp31), administered with incomplete freund's adjuvant, were evaluated in mice. immunization of balb/c mice with romp31 conferred protection against b. ovis and b. melitensis infection. romp31 induced a vigorous immunoglobulin g (igg) response, with higher igg1 than igg2 titers. in addition, spleen cells from romp31-immunized mice produced interleukin 2 (il-2) and gamma interfer ... | 2005 | 16299302 |
| whole-genome analyses of speciation events in pathogenic brucellae. | despite their high dna identity and a proposal to group classical brucella species as biovars of brucella melitensis, the commonly recognized brucella species can be distinguished by distinct biochemical and fatty acid characters, as well as by a marked host range (e.g., brucella suis for swine, b. melitensis for sheep and goats, and brucella abortus for cattle). here we present the genome of b. abortus 2308, the virulent prototype biovar 1 strain, and its comparison to the two other human patho ... | 2005 | 16299333 |
| high susceptibility of human dendritic cells to invasion by the intracellular pathogens brucella suis, b. abortus, and b. melitensis. | bacteria from the brucella genus are able to survive and proliferate within macrophages. because they are phylogenetically closely related to macrophages, myeloid dendritic cells (dcs) constitute potential targets for brucella bacteria. here we report that dcs display a great susceptibility to brucella infection. therefore, dcs might serve as a reservoir and be important for the development of brucella bacteria within their host. | 2005 | 16299342 |
| unraveling brucella genomics and pathogenesis in immunocompromised irf-1-/- mice. | brucellosis causes abortion in domestic animals and malta fever in humans. comparison of brucella species genomes may reveal potential virulence mechanisms. engineering bioluminescent brucella would permit monitoring bacterial dissemination. | 2005 | 16305661 |
| [brucellosis as an imported disease in a young man with arthritis]. | a 23-year-old man was admitted to hospital with an extremely painful sacroiliitis on the left side. he had been back in the netherlands for 5 weeks after a one-year stay on a farm in india where he consumed unpasteurised cow's milk almost daily. three months before admission he had been ill for several weeks with high fever, a painful right hip joint and night sweats. he was diagnosed in india as having coxitis, thought to be due to a giardia infection. after hospitalisation in the netherlands, ... | 2005 | 16385835 |
| the effect of long-term ethanol feeding on efficacy of doxycycline plus rifampicin in the treatment of experimental brucellosis caused by brucella melitensis in rats. | a model of brucella melitensis infection was used in the setting of long-term ethanol administration to study the effects of ethanol on antibiotic therapy of b. melitensis infection. wistar rats received a liquid diet containing maximally 42.2% of total calories as ethanol. controls were pair-fed a liquid diet without ethanol. diets began 15 days pre- and continued post-infection. rats were infected intraperitoneally with b. melitensis. doxycycline (10 mg/kg/day) plus rifampicin (6 mg/kg/day) we ... | 2005 | 16323439 |
| brucellar spinal epidural abscesses. analysis of eleven cases. | brucellar spinal epidural abscesses (bsea) are rare and very few series of them have been reported. in order to evaluate the clinical characteristics, management and outcome of this entity, the clinical records and current status of a series of 11 patients have been retrospectively reviewed. a series of 11 patients treated for bsea in our service during a period of 12 years (1989-2000) have been retrospectively studied. spinal epidural abscesses (sea) were diagnosed by mri, ct or at surgery. bru ... | 2005 | 16455524 |
| brucellosis: a rare cause of fever. | 2005 | 16398847 | |
| essential role of vaccines in brucellosis control and eradication programs for livestock. | brucellosis, in particular infections with brucella abortus, brucella melitensis or brucella suis, remains a significant human health threat in many areas of the world. the persistence of pathogenic brucella spp. in domestic livestock or free-ranging wildlife remains unresolved, despite decades of regulatory efforts worldwide. although vaccination is probably the most economic control measure, administration of currently available vaccines alone is not sufficient for elimination of brucellosis i ... | 2005 | 16372886 |
| primary brucellar psoas abscess: presentation of a rare case of psoas abscess caused by brucella melitensis without any osteoarticular involvement. | the psoas abscess is an entity, sometimes forgotten in our daily practice, because of infrequency and difficulties in diagnosis. primary psoas abscess is very rare and gram-positive micro-organisms account for more than 80% of the cases. our case, a 62-year-old man was admitted with a 5-year history of back pain and fever. physical examination was normal, except a palpable hepatomegaly with a mild tenderness over his lower right abdominal quadrant. the brucella agglutination test was strongly po ... | 2005 | 15875628 |
| brucellosis in san diego: epidemiology and species-related differences in acute clinical presentations. | although aggressive public health measures have greatly reduced the number of brucellosis cases in the united states, there is a resurgence of interest in this worldwide zoonosis because of its potential as a bioweapon and its 8-fold higher incidence in california, texas, and the other borderlands between the united states and mexico compared with the national rate. accordingly, we reviewed the clinical records of 28 patients diagnosed at a university hospital in san diego, ca, between 1979 and ... | 2005 | 15879907 |
| correlation of antigenic expression with progress in antibiotic therapy of acute human brucellosis. | human brucellosis is a zoonotic disease which is endemic in saudi arabia. the aim of this study was to investigate the humoral immune responses and identify the target antigens that persist at different stages in human brucellosis during antibiotic therapy. to do this, an acute case of accidental nosocomial infection was studied experimentally. blood was collected from the patient at the time of diagnosis, and at weekly intervals during therapy until remission. igg and igm immunoblotting was use ... | 2005 | 15888460 |
| a model of animal-human brucellosis transmission in mongolia. | we developed a dynamic model of livestock-to-human brucellosis transmission in mongolia. the compartmental model considers transmission within sheep and cattle populations and the transmission to humans as additive components. the model was fitted to demographic and seroprevalence data (rose bengal test) from livestock and annually reported new human brucellosis cases in mongolia for 1991-1999 prior to the onset of a mass livestock-vaccination campaign (s19 brucella abortus for cattle and rev 1 ... | 2005 | 15899298 |
| brucellosis. | 2005 | 15930423 | |
| comparison of milk-elisa and serum-elisa for the diagnosis of brucella melitensis infection in sheep. | milk and blood samples from 704 lactating ewes were examined for the diagnosis of brucella melitensis infection by milk-elisa, serum-elisa, rbpt, sat and culture of milk. of these ewes, 209 were from brucellosis free sheep flock, 443 from brucellosis infected sheep flock and 52 were from private sheep flocks of which status for brucellosis was not known. all the 209 ewes belonging to uninfected sheep flock were found negative in all the tests and of the remaining 495 ewes 105 were positive in se ... | 2005 | 15939556 |
| mixed cryoglobulinemia with renal failure, cutaneous vasculitis and peritonitis due to brucella melitensis. | cryoglobulinemia has been described in infectious diseases, but in only three patients with brucellosis. we report a 59-year-old male with brucella peritonitis with cutaneous vasculitis and renal failure that could be related to mixed cryoglobulinemia. as in cryoglobulinemia associated with other infections, resolution of the disease was obtained with specific antimicrobial therapy. | 2005 | 15951018 |
| human brucellosis in a nonendemic country: a report from germany, 2002 and 2003. | human brucellosis has become a rare disease in germany since the eradication of bovine and ovine/caprine brucellosis in this country. therefore, most physicians are unfamiliar with the illnesses clinical presentation, diagnostic tools, and therapeutic strategies. this retrospective study was carried out to evaluate the epidemiological, clinical, and laboratory features of human brucellosis in germany in the years 2002 and 2003. thirty-one bacterial isolates from 30 patients sent to the german na ... | 2005 | 15959815 |
| protective properties of rifampin-resistant rough mutants of brucella melitensis. | vaccination against brucella infections in animals is usually performed by administration of live attenuated smooth b. abortus strain s19 and b. melitensis strain rev1. they are proven effective vaccines against b. abortus in cattle and against b. melitensis and b. ovis in sheep and goats, respectively. however, both vaccines have the main drawback of inducing o-polysaccharide-specific antibodies that interfere with serologic diagnosis of disease. in addition, they retain residual virulence, bei ... | 2005 | 15972510 |
| citrate synthase mutants of agrobacterium are attenuated in virulence and display reduced vir gene induction. | a citrate synthase (cs) deletion mutant of agrobacterium tumefaciens c58 is highly attenuated in virulence. the identity of the mutant was initially determined from its amino acid sequence, which is 68% identical to escherichia coli and 77% identical to brucella melitensis. the mutant lost all cs enzymatic activity, and a cloned cs gene complemented a cs mutation in sinorhizobium. the cs mutation resulted in a 10-fold reduction in vir gene expression, which likely accounts for the attenuated vir ... | 2005 | 15995199 |
| [occupational brucellosis in slaughtering of sheep and goats: study of five cases from a municipal abattoir in south-eastern sicily]. | brucellosis is a world-wild zoonosis of bacterial origin. in italy, where reporting of the disease is mandatory, the incidence is 2.1 cases every 100,000 inhabitants, but tends to be higher in southern regions. however, the incidence decreased from 1999 to 2002. | 2005 | 16001513 |
| a quorum-sensing regulator controls expression of both the type iv secretion system and the flagellar apparatus of brucella melitensis. | both a type iv secretion system and a flagellum have been described in brucella melitensis. these two multimolecular surface appendages share several features. their expression in bacteriological medium is growth curve dependent, both are induced intracellularly and are required for full virulence in a mouse model of infection. here we report the identification of vjbr, a quorum sensing-related transcriptional regulator. a vjbr mutant has a downregulated expression of both virb operon and flagel ... | 2005 | 16008582 |
| adaptor long-range pcr procedure for clone-specific characterization and chromosomal localization. | an efficient adaptor long-range pcr (alr-pcr) procedure was developed to detect genomic rearrangements in high-plasticity genomic regions between closely related strains of bacteria. the method was precisely optimized using a combination of high-speed experimental steps for the chromosomal localization and elucidation of deletions, inversions, duplications, or inserted sequences within a clone-specific flanking region. the advantages of this strategy are: (i) ready-to-use polymerase mixtures and ... | 2005 | 16018549 |
| unusual presentation of brucellosis in a child: acute blindness. | in brucellosis, visual impairment due to optic nerve involvement is rare, and acute onset visual loss is an unusual presenting feature. we report a 15-y-old girl who had pancytopenia and who was admitted to our hospital with acute onset of bilateral blindness and fever. there was no light perception, and anterior segment and fundus examination were normal in both eyes. no other abnormal neurological findings were detected. increased latencies and decreased amplitudes were found in visual evoked ... | 2005 | 16028662 |
| role of trehalose transport and utilization in sinorhizobium meliloti--alfalfa interactions. | genes thua and thub in sinorhizobium meliloti rm1021 code for a major pathway for trehalose catabolism and are induced by trehalose but not by related structurally similar disaccharides like sucrose or maltose. s. meliloti strains mutated in either of these two genes were severely impaired in their ability to grow on trehalose as the sole source of carbon. thua and thub show no homology to any known enzymes in trehalose utilization. thua has similarity to proteins of unknown function in mesorhiz ... | 2005 | 16042015 |
| active membrane transport and receptor proteins from bacteria. | a general strategy for the expression of bacterial membrane transport and receptor genes in escherichia coli is described. expression is amplified so that the encoded proteins comprise 5-35% of e. coli inner membrane protein. depending upon their topology, proteins are produced with rgsh6 or a strep tag at the c-terminus. these enable purification in mg quantities for crystallization and nmr studies. examples of one nutrient uptake and one multidrug extrusion protein from helicobacter pylori are ... | 2005 | 16042616 |
| brucella melitensis infection associated with guillain-barré syndrome through molecular mimicry of host structures. | brucella melitensis is a facultative intracellular bacterium that can survive inside macrophages and the causative agent of brucellosis. in the present study, we found that a lipooligosaccharide of b. melitensis has a gm1 ganglioside-like structure and shows a strong antibody response in mice. the cholera toxin b subunit, which binds to gm1 ganglioside specifically, reacted with the surface of b. melitensis. immunization with b. melitensis induced the production of anti-gm1 ganglioside antibodie ... | 2005 | 16051063 |
| detection of brucella dna in sera from patients with brucellosis by polymerase chain reaction. | to overcome the limitations of current diagnostic methods for brucellosis, accurate and simple methods must be sought. in this study, sera of patients diagnosed with brucellosis were examined by polymerase chain reaction (pcr) for the detection of brucella dna. results showed that an amplicon of 223 bp was obtained in 96% (24/25) of tested sera using primers derived from the nucleotide sequence of a gene encoding the 31-kda brucella abortus antigen. however, when serum dna templates were examine ... | 2005 | 16054326 |
| time is up! | 2005 | 16082998 | |
| systematic targeted mutagenesis of brucella melitensis 16m reveals a major role for gntr regulators in the control of virulence. | in order to identify transcriptional regulators involved in virulence gene control in brucella melitensis, we generated a collection of 88 mutants in the arac, arsr, crp, deor, gntr, iclr, lysr, merr, rpir, and tetr families of regulators. this collection was named limur (library of mutants for regulators). we developed a method to test several mutants simultaneously in one animal in order to identify those unable to survive. this method, called the plasmid-tagged mutagenesis method, was used to ... | 2005 | 16113274 |
| brucella abortus virb12 is expressed during infection but is not an essential component of the type iv secretion system. | the brucella abortus virb operon, consisting of 11 genes, virb1 to virb11, and two putative genes, orf12 (virb12) and orf13, encodes a type iv secretion system (t4ss) that is required for intracellular replication and persistent infection in the mouse model. this study was undertaken to determine whether orf12 (virb12) encodes an essential part of the t4ss apparatus. the virb12 gene was found to encode a 17-kda protein, which was detected in vitro in b. abortus grown to stationary phase. mice in ... | 2005 | 16113325 |
| rifampin induced arthritis. | 2005 | 16125781 | |
| brucella prostatic abscess: first case report. | brucellosis is a multi-system disease that is endemic in many mediterranean countries including lebanon. however, human brucella prostatic abscess was not reported in the english medical literature. we here describe the case of a young man who presented with fever and urinary symptoms, and who subsequently proved to have culture confirmed brucella prostatic abscess. the culture of drained pus grew brucella melitensis. | 2005 | 16126573 |
| lumbosacral spinal epidural abscess caused by brucella melitensis. | spinal epidural abscess is a rare condition that can lead to irreversible complications and death if untreated. rarely, brucella melitensis may produce spondylodiscitis, which may be complicated by spinal epidural abscess. we report a case of lumbosacral spinal epidural abscess caused by brucella melitensis. spinal brucellosis should be kept in mind in the differential diagnosis of low back pain. | 2005 | 16133775 |
| brucella melitensis in a married couple after a trip through sicily. | 2005 | 16144189 | |
| brucellosis. | 2005 | 16148300 | |
| temporal analysis of pathogenic events in virulent and avirulent brucella melitensis infections. | despite progress in mouse models of brucellosis, much remains unknown regarding brucella dissemination and tissue localization. here, we report the dynamics of brucella infection in individual mice using bioluminescent brucella melitensis. bioluminescent imaging of infected interferon regulatory factor-1 knockout (irf-1(-/-)) mice identified acute infection in many tissues. brucella was found to replicate in the salivary glands of irf-1(-/-) and wild-type c57bl/6 mice suggesting a previously unk ... | 2005 | 16153245 |
| brucellosis. | 2005 | 16156056 | |
| [new diagnostic methods for bacterial infections after the introduction of increased bioterrorism preparedness]. | bacillus anthracis, francicella tularensis, yersinia pestis, burkholderia pseudomallei and brucella melitensis are the bacteria most often mentioned in connection with possible biological terrorist acts. it is known that these microorganisms can be cultured and weaponised for such a purpose. this survey provides examples of the use of diagnostic methods for those microorganisms, further detailed in relation to establishing a national center for biological preparedness in denmark. | 2005 | 16159496 |
| neurobrucellosis presenting as an intra-medullary spinal cord abscess. | of the diverse presentation of neurobrucellosis, intra-medullary spinal cord abscess is extremely rare. only four other cases have been reported so far. we present a case of spinal cord intra-medullary abscess due to brucella melitensis. | 2005 | 16168059 |
| a dna vaccine coding for the brucella outer membrane protein 31 confers protection against b. melitensis and b. ovis infection by eliciting a specific cytotoxic response. | the development of an effective subunit vaccine against brucellosis is a research area of intense interest. the outer membrane proteins (omps) of brucella spp. have been extensively characterized as potential immunogenic and protective antigens. this study was conducted to evaluate the immunogenicity and protective efficacy of the b. melitensis omp31 gene cloned in the pci plasmid (pciomp31). immunization of balb/c mice with pciomp31 conferred protection against b. ovis and b. melitensis infecti ... | 2005 | 16177328 |
| cloning and sequencing of 28 kda outer membrane protein gene of brucella melitensis rev. 1. | brucella melitensis is an organism of paramount zoonotic importance. the 28 kda outer membrane protein (omp) is one of the immunodominant antigens of b. melitensis. the gene encoding 28 kda omp (omp28) has been amplified from b. melitensis rev. 1 strain. a pcr product of 753 bp, encoding complete omp28 gene of b. melitensis, was obtained. the gene was further cloned and sequenced. the nucleotide sequence of b. melitensis rev. 1 strain showed substitution of 2 nucleotides from that of 16m strain. | 2005 | 16187538 |
| use of shell-vial cell culture assay for isolation of bacteria from clinical specimens: 13 years of experience. | the shell-vial culture assay is performed routinely in our laboratory. recently we revisited our experience of using the shell-vial culture assay for the isolation of microorganisms from various clinical samples. over a 13-year period, we have isolated 580 bacterial strains (5%) from 11,083 clinical samples tested. over the same period, 285 isolates of rickettsiae, bartonellae, or coxiella burnetii were cultured from a total of 7,102 samples tested. these isolates include 55 rickettsia sp. isola ... | 2005 | 16207953 |
| epididymoorchitis due to brucellosis in central anatolia, turkey. | brucellosis may involve many organs and tissues. epididymoorchitis is a focal genitourinary complication of human brucellosis. in this study, we describe our experience with the diagnosis, treatment, and final outcomes of 17 patients with epididymoorchitis out of 186 male patients with brucellosis between march 1999 and december 2003. the rate of epididymoorchitisdue to brucellosiswas 9.1%. all subjects complained about swollen, painful testicles. the duration of their complaint varied between 1 ... | 2005 | 16215312 |
| reliability evaluation of sampling plan fixed by council directive 91/68/eec for the maintenance of officially brucellosis-free flock status. | the european union (eu) strategy with respect to sheep and goat brucellosis aims to eradicate the infection and achieve officially brucellosis-free (obf) status in all eu holdings and territories. council directive 91/68/eec of 28 january 1991 states that to maintain obf status of ovine or caprine holdings located outside an obf territory, only a representative number of animals need to be tested annually. however, depending on the number of animals in a holding, this testing method risks non-de ... | 2005 | 16219092 |
| a case of human brucellosis in hong kong. | brucellosis is an infectious disease of humans and animals caused by brucella species. we report on a 34-year-old housewife who presented with recurrent headache, fever, and malaise. blood cultures yielded slow-growing gram-negative coccobacilli that were later identified as brucella melitensis. the patient recalled handling goat placenta in china. she was prescribed a 6-week course of doxycycline and rifampicin. laboratory staff who had been exposed to the isolate remained asymptomatic. the epi ... | 2005 | 16219962 |
| in vitro activities of various antimicrobials against brucella melitensis strains in the aegean region in turkey. | to study in vitro activities of three quinolones (ciprofloxacin, levofloxacin, moxifloxacin), four macrolides (erythromycin, dirithromycin, azithromycin, clarithromycin) and doxycycline against 44 clinical isolates of brucella melitensis. | 2005 | 16220015 |
| evolutionary, structural and functional relationships revealed by comparative analysis of syntenic genes in rhizobiales. | comparative genomics has provided valuable insights into the nature of gene sequence variation and chromosomal organization of closely related bacterial species. however, questions about the biological significance of gene order conservation, or synteny, remain open. moreover, few comprehensive studies have been reported for rhizobial genomes. | 2005 | 16229745 |
| selection of protective epitopes for brucella melitensis by dna vaccination. | the brucella melitensis 16m genome was examined for proteins in excess of 100 amino acids and for immunogenicity-associated genes. one subset of 32 annotated genes or open reading frames was identified, and each of these were cloned into the eukaryotic vector pcdna3.1. purified recombinant plasmids were used to intramuscularly (i.m.) immunize balb/c mice. after challenge with b. melitensis 16m strain, two protective antigens were found: the periplasmic protein, bp26, and the chaperone protein, t ... | 2005 | 16239526 |
| recurrent rhabdomyolysis and mild acute renal failure associated with acute brucella infection. | 2005 | 15716295 | |
| the effect of the doxycycline-rifampicin and levamisole combination on lymphocyte subgroups and functions of phagocytic cells in patients with chronic brucellosis. | brucellosis is one of the important health problems for both humans and animals in turkey since agriculture and stock raising appears to be the most important means of subsistence. investigations on the pathogenesis of brucellosis reveal that the etiologic agent can survive in phagocytic cells, and cell-mediated immunity plays an important role in immunity against bacteria. | 2005 | 15722630 |
| immunogenicity of major cell surface protein(s) of brucella melitensis rev 1. | brucella melitensis rev 1 organisms were salt-extracted and the cell surface proteins (bcsps) were found to be mainly 39-42 kda (group 2 porin proteins) in addition to 31.6, 32.5, 58.5 and 14.7 kda proteins. deae-sephadex anion-exchange column chromatography of bcsps yielded fraction 1, which contained one major protein (39.8-42.0 kda) and a minor protein (31.6 kda). all these proteins were found to be immunogenic by western blotting. fraction 1 along with monophosphoryl lipid a and trehalose di ... | 2005 | 15736854 |
| the use of homologous antigen in the serological diagnosis of brucellosis caused by brucella melitensis. | in the european union the serological diagnosis of brucellosis caused by brucella melitensis is performed using the heterologous antigen of b. abortus s99. the possible higher sensitivity or ability of an early detection of antibodies by a homologous antigen may prove very useful in the final phases of an eradication programme. results obtained in sheep experimentally infected by b. melitensis biovar 3 were compared using b. abortus s99, b. melitensis m1, m2 and m3 antigens in the rose bengal pl ... | 2005 | 15752266 |
| bacterial zoonoses and infective endocarditis, algeria. | blood culture-negative endocarditis is common in algeria. we describe the etiology of infective endocarditis in this country. samples from 110 cases in 108 patients were collected in algiers. blood cultures were performed in algeria. serologic and molecular analysis of valves was performed in france. infective endocarditis was classified as definite in 77 cases and possible in 33. causative agents were detected by blood cultures in 48 cases. all 62 blood culture-negative endocarditis cases were ... | 2005 | 15752438 |
| [non infectious endocarditis: retrospective study (6 cases)]. | if there is cardiac valve vegetation and the blood cultures are negatives we need to look for slow growing bacteria, fungi, legionella pneumophilia, bartonella henselae and quintana, brucella melitensis and abortus, coxiella burneti, chlamydiae pneumoniae by serologic tests. the diagnosis of non- infectious endocarditis could be considered only if these results were negative. the main purpose of this study was to describe the clinical and echocardiographic signs of non-infectious endocarditis ca ... | 2005 | 15777581 |
| abdominal aortic aneurysm due to brucella melitensis. | 2005 | 15790211 | |
| interferon-gamma associated cytokines and chemokines produced by spleen cells from brucella-immune mice. | it is known that interferon (ifn)-gamma plays a critical role in protection against brucellosis. in this study we have investigated several cytokines and chemokines that are associated with ifn-gamma for potential in vitro correlates of protection. we cultured spleen cells in vitro from mice immunized orally with a live, attenuated brucella melitensis vaccine candidate (wr201) and stimulated these cells with a lysate of b. melitensis. differential gene expression of several cytokines and chemoki ... | 2005 | 15804600 |
| completion of the genome sequence of brucella abortus and comparison to the highly similar genomes of brucella melitensis and brucella suis. | brucellosis is a worldwide disease of humans and livestock that is caused by a number of very closely related classical brucella species in the alpha-2 subdivision of the proteobacteria. we report the complete genome sequence of brucella abortus field isolate 9-941 and compare it to those of brucella suis 1330 and brucella melitensis 16 m. the genomes of these brucella species are strikingly similar, with nearly identical genetic content and gene organization. however, a number of insertion-dele ... | 2005 | 15805518 |
| the sheathed flagellum of brucella melitensis is involved in persistence in a murine model of infection. | persistence infection is the keystone of the ruminant and human diseases called brucellosis and malta fever, respectively, and is linked to the intracellular tropism of brucella spp. while described as non-motile, brucella spp. have all the genes except the chemotactic system, necessary to assemble a functional flagellum. we undertook to determine whether these genes are expressed and are playing a role in some step of the disease process. we demonstrated that in the early log phase of a growth ... | 2005 | 15839898 |
| from the discovery of the malta fever's agent to the discovery of a marine mammal reservoir, brucellosis has continuously been a re-emerging zoonosis. | brucellosis is not a sustainable disease in humans. the source of human infection always resides in domestic or wild animal reservoirs. the routes of infection are multiple: food-borne, occupational or recreational, linked to travel and even to bioterrorism. new brucella strains or species may emerge and existing brucella species adapt to changing social, cultural, travel and agricultural environment. brucella melitensis is the most important zoonotic agent, followed by brucella abortus and bruc ... | 2005 | 15845228 |
| genomic subtraction followed by dot blot screening of streptococcus pneumoniae clinical and carriage isolates identifies genetic differences associated with strains that cause otitis media. | streptococcus pneumoniae strains are the leading cause of bacterial otitis media, yet little is known about specific bacterial factors important for this disease. we utilized a molecular epidemiological approach involving genomic subtraction of the s. pneumoniae serogroup 19 middle ear strain 5093 against the laboratory strain r6. resulting subtraction pcr (spcr) products were used to screen a panel of 93 middle ear, 90 blood, 35 carriage, and 58 cerebrospinal fluid isolates from young children ... | 2005 | 15845484 |
| in vitro susceptibility of clinical isolates of brucella melitensis to fucidic acid. | brucella species are facultative intracellular bacteria, and therefore a limited number of antibiotics are effective against these organisms. the side effects of drug combination schemes, and the incidences of relapses and therapeutic failures, have led to investigations of new drugs to treat brucellosis. the purpose of this study was to test the in vitro susceptibility of 50 brucella melitensis isolates to fucidic acid, which has not previously been used for the treatment of brucellosis. the mi ... | 2005 | 15856380 |
| brucella melitensis in blood cultures of two newborns due to exchange transfusion. | brucellosis is a zoonotic infection, transmitted to humans primarily by consumption of unpasteurized milk and milk products. transmission by blood transfusion is possible but very unusual. herewith we present two newborns with positive blood cultures for brucella melitensis after exchange transfusions. however, the standard tube agglutination titers against brucella were not elevated and the newborns did not develop brucellosis. it is suggested that, in areas endemic for brucellosis, blood donor ... | 2005 | 16250315 |
| spontaneous bacterial peritonitis due to brucella melitensis in a cirrhotic patient. | spontaneous bacterial peritonitis is a well-known entity, with a reported incidence of 15-20% in advanced cirrhotic patients. escherichia coli and klebsiella pneumoniae are the most common causes of spontaneous bacterial peritonitis; brucella is extremely rare. we aimed to present one case of such a rare condition in a cirrhotic patient who also had hepatocellular carcinoma. routine laboratory tests, abdominal ultrasonography and peritoneal fluid examinations were studied in a cirrhotic patient ... | 2005 | 16252187 |
| imported brucellosis associated with plasmodium falciparum malaria in a traveler returning from the tropics. | we report a microbiologically confirmed case of brucella melitensis and plasmodium falciparum malaria coinfection in a febrile traveler returning from chad, africa. the patient had been doing veterinary research in rural chad; during that time she took no antimalarial chemoprophylaxis. our report highlights the importance of blood cultures as well as malaria smears in febrile travelers returning from the tropics. | 2005 | 16256053 |
| evaluation of culture, tube agglutination, and pcr methods for the diagnosis of brucellosis in humans. | brucellosis is prevalent in saudi arabia and brucela melitensis is a leading cause of zoonosis worldwide. therefore, accurate diagnosis of brucellosis is a key to its treatment and control. | 2005 | 16258407 |
| relapsing brucellosis related to pacemaker infection. | infection is a serious complication following pacemaker implantation. usually it results from normal flora of the skin or from enterobacteriaceae, pseudomonas aeruginosa, streptococcus spp., enterococcus spp. we report here a case suggesting that brucella melitensis is able to persist around pacemaker device being a cause of relapsing brucellosis. | 2005 | 16274028 |
| combined brucella melitensis and staphylococcus aureus endocarditis in a patient with a prosthetic aortic valve with fatal outcome. | 2005 | 16275556 | |
| validation of fluorescence polarization assay (fpa) and comparison with other tests used for diagnosis of b. melitensis infection in sheep. | fluorescence polarization assay (fpa) is a new test for the serological diagnosis of brucella spp. infection in animals. the fpa is validated for the diagnosis of b. melitensis infection in sheep. for this purpose, 166 sera originated from natural infected sheep (verified by culture) and 851 sera originated from healthy animals (reared in areas where b. melitensis was never been isolated) were tested. the optimum cut-off value that offers the highest diagnostic sensitivity (dsn) and diagnostic s ... | 2005 | 16278056 |