Publications

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clinical and microbiological effect of scaling and root planing in smoker and non-smoker chronic and aggressive periodontitis patients.to compare the effects of scaling and root planing (srp) on clinical and microbiological parameters at selected sites in smoker and non-smoker chronic and generalized aggressive periodontitis patients.200515691352
[efficacy variation of erythromycin and spiramycin on periopathogens in aggressive periodontitis. an in vitro comparative study].erythromycin (ery) and spiramycin (spi) are the most frequently prescribed macrolides by dentists. however, the emergence of resistant anaerobic subgingival bacteria imposes an increased vigilance. this study aims to compare these macrolides efficacy on principal periopathogens.200516087299
detection of bacteria in endodontic samples by polymerase chain reaction assays and association with defined clinical signs in italian patients.the presence of selected bacteria (enterococcus faecalis, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis, treponema denticola) in infected root canals was studied using polymerase chain reaction (pcr) assays, and the association of bacteria with clinical signs of endodontic disease was assessed. the null hypothesis, that no difference could be observed between clinical signs of apical periodontitis and a specific bacterial strain, was tested.200516101964
impact of smoking on the clinical, microbiological and immunological parameters of adult patients with periodontitis.the aim of the current study was to assess the impact of smoking on the clinical indices, the humoral immune response and the detection frequency of putative periodontal pathogens in patients with periodontitis cross-sectionally and following therapy.200516104962
antimicrobial resistance and beta-lactamase production of clinical isolates of prevotella and porphyromonas species.this study determined the beta-lactamase production and the antimicrobial resistance of 72 prevotella species and 48 porphyromonas species isolated from different clinical specimens.200515722627
combined effects of nd:yag laser irradiation with local antibiotic application into periodontal pockets.the purpose of the present study was to assess the effects of nd:yag laser irradiation into periodontal pockets with or without the combination of local antibiotic application on clinical parameters and microbiological prevalence. sixteen patients, each of whom had 4 or more sites with probing depth e x 4mm were included in this study. they were monitored clinically and microbiologically at baseline, 1 and 3 months after the treatment. subgingival plaque samples were taken from periodontally inv ...200515736890
using photodynamic therapy to treat peri-implantitis. interview. 200515747830
lethal photosensitization of oral pathogens via red-filtered halogen lamp.objective of investigation: the ability of laser irradiation in the presence of photosensitizing agent to induce lethal effect on oral bacteria is well documented. we designed an in-vitro experiment to achieve phototoxic results on two common oral pathogens, using a high intensity, red filtered halogen lamp. our goal was to determine the minimum duration of light exposure and drug dilution to achieve at least 50% reduction in bacteria counts.200515752111
phototargeting oral black-pigmented bacteria.we have found that broadband light (380 to 520 nm) rapidly and selectively kills oral black-pigmented bacteria (bpb) in pure cultures and in dental plaque samples obtained from human subjects with chronic periodontitis. we hypothesize that this killing effect is a result of light excitation of their endogenous porphyrins. cultures of prevotella intermedia and p. nigrescens were killed by 4.2 j/cm2, whereas p. melaninogenica required 21 j/cm2. exposure to light with a fluence of 42 j/cm2 produced ...200515793117
oral lactobacilli in chronic periodontitis and periodontal health: species composition and antimicrobial activity.lactobacilli are known to play an important role in the maintenance of health by stimulating natural immunity and contributing to the balance of microflora. however, their role in chronic periodontitis is unclear. we aimed to identify oral lactobacilli in chronic periodontitis and periodontally healthy subjects, and to determine their antimicrobial activity against putative oral pathogens.200516238595
treponema medium glycoconjugate inhibits activation of human gingival fibroblasts stimulated with phenol-water extracts of periodontopathic bacteria.oral treponemes are well-known as causative agents of periodontal diseases; however, the details have not been fully clarified. here, we examined the effects of treponema medium glycoconjugate on the activation of human gingival fibroblasts using phenol-water extracts from porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum subsp. nucleatum, and actinobacillus actinomycetemcomitans. the phenol-water extracts activated human gingival fibroblasts to mediate il-8 production, as ...200515840783
diabetes and periodontal disease: a case-control study.periodontitis is often associated with diabetes and might be considered one of the chronic complications of diabetes mellitus, both in type 1 (t1dm) and type 2 (t2dm). this case-control study was designed to evaluate the possible association between non-insulin-dependent diabetes (t2dm) and clinical and microbiological periodontal disease among adult sardinians.200515857077
implants in patients treated for generalized aggressive and chronic periodontitis: a 3-year prospective longitudinal study.the aim of the present prospective longitudinal study of periodontally diseased and periodontally healthy patients was a clinical, microbiological, and radiographic comparison of teeth and implants and an assessment of the implant success rate.200515857093
tobacco smoking and periodontal microflora in a saudi arabian population.to explore the subgingival periodontal microflora in a saudi arabian population with a special focus on its relationship with various smoking habits.200515882210
clinical and microbiological evaluation of the effectiveness of the nd:yap laser for the initial treatment of adult periodontitis. a randomized controlled study.enhancement of the results obtained by scaling and planing is most often sought by using antimicrobial therapies. laser beams have been shown to be bactericidal and could possibly target pathogens more effectively and with fewer compliance problems than antiseptic solutions.200515882229
susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, {beta}-defensins and ll37, produced by human epithelial cells.antimicrobial peptides are one of the factors involved in innate immunity. the susceptibility of periodontopathogenic and cariogenic bacteria to the major antimicrobial peptides produced by epithelia was investigated.200515886266
bacterial colonization on different suture materials--a potential risk for intraoral dentoalveolar surgery.in this in vivo and in vitro study on resorbable (monocryl and nonresorbable (deknalon) monofilament sutures used in intraoral dentoalveolar surgery the bacterial colonization was compared. for the in vivo study the sutures were applied in 11 patients during dental surgery. eight days postoperative the sutures were removed and the adhered bacteria were isolated and identified by biochemistry, morphology, antibiotic susceptibility, and gas chromatography. the colonization was studied by scanning ...200515889436
[pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology].this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria.200515757581
antimicrobial actions of benzimidazoles against oral streptococci.benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton-pump inhibitors to control stomach acid secretion and also have antimicrobial actions against helicobacter pylori. our objective was to determine whether they are potentially useful antimicrobials against oral bacteria.200515720569
identification of periodontal pathogens in atherosclerotic vessels.epidemiological studies have shown that periodontitis may be associated with presence of atherosclerosis. dna from periodontal pathogens has been detected in atherosclerotic lesions, but viable oral bacteria have not yet been isolated from atherosclerotic plaques. the purpose of the present study was to determine if viable oral bacteria could be isolated from atherosclerotic lesions and if dna from periodontal pathogens could be detected by use of polymerase chain reaction (pcr) techniques.200515898933
dna probe identification of bacteria colonizing internal surfaces of the implant-abutment interface: a preliminary study.currently, there is limited knowledge concerning the specific genus and species of bacteria that may colonize internal surfaces of the implant-abutment interface (iai) of two-stage dental implants. the purpose of this study was to use dna probe analysis to identify those periodontopathic bacteria that may inhabit the internal surfaces and healing abutment screw-threads of the iai of dental implants in situ.200515830645
detection of heat shock proteins but not superantigen by isolated oral bacteria from patients with behcet's disease.we isolated bacteria from periodontal sites and mixed saliva in eight patients with behcet's disease and surveyed them to determine whether they produced heat shock proteins (hsps) and superantigen. cultivable bacterial compositions from periodontal sites and saliva were examined by anaerobic culture using blood agar plates. gram-negative anaerobic rods such as prevotella intermedia, fusobacterium nucleatum, and capnocytophaga species were predominant in the isolates from the subgingival plaque ...200515836518
[bacterial spectra and antibiotics in odontogenic infections. renaissance of the penicillins?].the role played by odontogenic infection in dental, oral, and maxillofacial surgery is not to be underestimated even at the present time. an extensive, standardized, prospective study was performed with the intention of verifying the bacterial spectrum of odontogenic infections to evaluate antibiotic sensitivity.200516261393
clinical and microbiological characterization of periodontal abscesses.the knowledge of clinical features, microbial composition and susceptibility to antimicrobials of periodontal abscesses has recently improved. this descriptive clinical and microbiological study provides more information on the characteristics of periodontal abscesses.200516268997
debridement and local application of tetracycline in the management of persistent periodontitis. clinical and microbiological results after 12 months.the purpose of this study was to evaluate the clinical and microbiological efficacy of local delivery of tetracycline in persistent periodontal lesions after topical therapy.200515902062
[experience in medical ozone use for root canal treatment].the results of clinical and laboratory assessment of effectiveness of psr-diagnosis are presented. the high efficacy of ozone therapy is revealed. the findings demonstrate the reduction of number in the micro-organisms in root canal: actinobacillus actinomycetemcomitans from 31.25% to 10.21%; bacteriodes forsythus from 68.75% to 15.50%; treponema denticola from 37.5% to 11.4%; porphyromonas gingivalis from 56.25% to 45%; prevotella intermedia from 16% to 0%.200516353031
periodontal pathogens: a quantitative comparison of anaerobic culture and real-time pcr.periodontitis is a multi-factorial chronic inflammatory and destructive disease of the tooth-supporting tissues. quantitative anaerobic culture techniques have been used for microbial diagnosis of the different forms of the disease. the aim of this study was to compare real-time pcr with quantitative anaerobic culture for detection and quantification of 5 prominent periodontal pathogens. real-time pcr assays with the 16s rrna genes of actinobacillus actinomycetemcomitans, prevotella intermedia, ...200515919188
serum antibodies to oral anaerobic bacteria in patients with rheumatoid arthritis.this study was conducted to determine the component that causes the disease in rheumatoid arthritis (ra), which shows great resemblance to periodontitis in a pathologic context.200516369381
porphyromonas gingivalis, porphyromonas endodontalis, prevotella intermedia and prevotella nigrescens in endodontic lesions detected by culture and by pcr.he aim of this study was to investigate the presence of four black-pigmented bacteria, porphyromonas gingivalis, porphyromonas endodontalis, prevotella intermedia and prevotella nigrescens, in endodontic infections by culture and polymerase chain reaction (pcr) analyses. microbial samples were obtained from 50 teeth with untreated necrotic pulps (primary infection) and from 50 teeth with failing endodontic treatment (secondary infection). microbiological strict anaerobic techniques were used for ...200515943764
antimicrobial susceptibility variation of 50 anaerobic periopathogens in aggressive periodontitis: an interindividual variability study.the frequent use of antibiotics in developed countries has led to the emergence of widespread bacterial resistance. in this study, the interindividual variability of the antibiotic susceptibility of 50 putative microorganisms in aggressive periodontitis patients has been evaluated by means of vc (variation coefficient).200515943770
analysis of the presence of pathogens which predict the risk of disease at peri-implant sites through polymerase chain reaction (pcr).the presence of dna of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia in the peri-implant sulcus samples of 19 partially edentulous patients was analyzed by polymerase chain reaction (pcr) and related to the depth of the peri-implant sulcus, bleeding on probing, and probable risk of disease. ten of those patients presented a history of periodontal disease and nine of those did not. the dna amplification of these pathogens was observed in seven samples, ...200516229357
effects of zinc and copper on adhesion and hemagglutination of prevotella intermedia and prevotella nigrescens.this study investigated the mechanism of protein attachment to the surface of the putative periodontal pathogens prevotella intermedia and prevotella nigrescens in artificial gingival crevicular fluid, and ways to increase protein attachment to the bacterial cells. the effects of cations on protein attachment, bacterial adhesion, and hemagglutination were examined, and cation-binding components on both bacterial species were identified. the presence of cations, especially zinc, copper and cerium ...200516238592
antimicrobial effect in vitro of chlorhexidine and calcium hydroxide impregnated gutta-percha points.the aim of this study was to evaluate the antimicrobial activity of points containing antiseptics used for temporary obturation in endodontics. points containing calcium hydroxide (roeko and hygienic), and chlorhexidine (roeko) were tested and gutta-percha points served as control (meta dental corp.). the following microorganisms were studied: streptococcus mutans (sm), enterococcus faecalis (ef), staphylococcus aureus (sta), candida albicans (ca), fusobacterium nucleatum (fn) atcc 25586, porphy ...200516673792
the association of anaerobic bacteria with infectious mononucleosis.this review summarizes the information that supports the potential importance of anaerobic bacteria in the pharyngo-tonsillitis (pt) associated with infectious mononucleosis (im). evidence supportive of the potential of involvement of bacteria in im was provided by several studies that showed increased recovery of bacteria on the tonsillar surfaces in im. several studies demonstrated that metronidazole therapy alleviated the clinical symptoms of tonsillar hypertrophy and shortened the duration o ...200516701590
periodontal pathogens in atheromatous plaques isolated from patients with chronic periodontitis.it has been suggested that chronic infections may predispose to cardiovascular disease. the relationship between periodontal disease and cardiovascular disease has been a subject of increasing research in recent years. the isolation and identification of periodontal bacteria from atheromatous plaque can contribute to our knowledge of this vascular disease. the aim of this study was to isolate and identify periodontal bacteria from the periodontal pockets of different patients and to compare them ...200616827731
effectiveness of ultrasonic instruments in the therapy of severe periodontitis: a comparative clinical-microbiological assessment with curettes.patients with deep periodontal pockets were treated with either vector system (tg) or manual instruments (cg). clinical assessments by supragingival plaque (pl+), gingival index (gi), bleeding on probing (bop), probing depth (pd), clinical attachment level (cal) and subgingival plaque collection for microbiological analysis were made prior to and after treatment. multiplex polymerase chain reaction was used to determine the presence of actinobacillus actinomycetemcomitans, campylobacter rectus, ...200616841550
inflammation, heat shock proteins and periodontal pathogens in atherosclerosis: an immunohistologic study.inflammation is a significant component of atherosclerosis lesions. bacteria, including periodontopathogens, have been demonstrated in atherosclerotic plaques and cross-reactivity of the immune response to bacterial groel with human heat shock protein 60 has been suggested as a link between infections and atherosclerosis.200616842503
antibody response after single-visit full-mouth ultrasonic debridement versus quadrant-wise therapy.the aim of this study was to compare serum antibody responses to periodontal pathogens after single-visit full-mouth ultrasonic debridement and quadrant-wise therapy.200616856899
one stage full-mouth disinfection--treatment of choice? 200617092248
selective inhibition of porphyromonas gingivalis growth by a factor xa inhibitor, dx-9065a.porphyromonas gingivalis is a causative bacterium of adult periodontitis. however, there is no drug specific for p. gingivalis and for its virulence factor.200616677284
the detection of porphyromonas gingivalis, prevotella intermedia and actinobacillus actinomycetemcomitans in tooth, tongue and buccal mucosa plaques in children, using immunoslot blot assay (iba).the present study was to investigate the distribution of typical periodontpathic bacteria (porphyromonas gingivalis, prevotella intermedia and actinobacillus actinomycetemcomitans) in tooth, tongue and buccal mucosa plaques in 3 to 17 year old children. clinical parameters (rates of df, d, dmf, and d; plaque and gingival index) for each subject were determined prior to the collection of each site plaque. three periodontopathic bacteria on each site samples were detected using iba. the frequency ...200616683675
in vitro antibacterial efficacy of a new chlorhexidine slow-release device.the aim of this study was to evaluate and compare the antibacterial effect of chlorhexidine and calcium hydroxide slow-release devices on oral bacteria.200616683687
red autofluorescence of dental plaque bacteria.red autofluorescence of plaque and its relation to fluorescence of a single species in the biofilm was studied. fluorescence images of non-disclosed and disclosed plaque of 28 first-year students were captured. the plaque samples were assessed by culture methods and studied for red autofluorescence. species capable of producing red autofluorescence were cultivated from subjects with and without red plaque autofluorescence. red autofluorescence was observed from actinomyces odontolyticus, prevote ...200617063027
antibacterial action of photoactivated disinfection {pad} used on endodontic bacteria in planktonic suspension and in artificial and human root canals.to measure antibacterial action of photoactivated disinfection (pad) on endodontic bacteria in planktonic suspension and root canals. methods: four bacteria, fusobacterium nucleatum,peptostreptococcus micros, prevotella intermedia and streptococcus intermedius, were tested in suspension. after mixing equal volumes of tolonium chloride and bacterial suspension for 60s, each 200 microl of concentration (>10(6)cfu ml(-1)) was irradiated with light at 633+/-2 nm. each energy dose/tolonium chloride c ...200616239058
porphyromonas gingivalis-induced cellular hypertrophy and mmp-9 activity via different signaling pathways in h9c2 cardiomyoblast cells.little is known about the pathogenesis of cardiomyocyte hypertrophy caused by periodontitis pathogens. the purpose of this study was to determine the effect of the periodontal pathogen porphyromonas gingivalis on cardiomyocyte hypertrophy.200616584351
langerhans' cell histiocytosis in a 5-year-old girl: evidence of periodontal pathogens.langerhans' cell histiocytosis (lch) is a rare disorder characterized by langerhans' cell proliferation in various organs or tissues. when periodontal tissue is involved, clinical manifestations can vary from gingival recession and pocket formation to severe alveolar bone loss. this case report describes periodontal pathogens found in the pockets of involved primary teeth.200616584357
[microbiological study on the prognosis of immediate implant and periodontal disease].the aim of the present study was to investigate the prevalence of periodontal marker organisms and specific interleukin-1 (il-1) gene polymorphisms (which show a close association with periodontitis) and their effect on the success of immediate implant placement postextraction in the patient with periodontal disease.200616395549
recovery of anaerobic bacteria from a case of dissecting cellulitis. 200616445513
characterization of patients presenting for treatment to a university refractory periodontal diseases unit: three case reports.of the many forms of periodontal disease, refractory periodontal diseases are the least characterized. they are defined as the continued degeneration of the periodontium despite adequate management. this has led to the suggestion that there may be a systemic component that is a contributing factor to the development of this condition. the objectives of this report were to follow the progression of clinical changes associated with periodontal disease over a number of years in this unique populati ...200616460260
molecular analysis of bacteria in asymptomatic and symptomatic endodontic infections.the purpose of the present study was to use terminal restriction fragment length polymorphism analysis and the 16s rrna gene clone library to investigate the diversity of the microbiota associated with asymptomatic and symptomatic endodontic infections and to compare the bacterial community structure in these two clinical conditions. samples were taken from asymptomatic endodontic infections associated with chronic periradicular lesions and from symptomatic infections clinically diagnosed as acu ...200616476021
the efficacy of povidone-iodine products against periodontopathic bacteria.a total of 8 strains of 6 bacterial species, porphyromonas gingivalis atcc33277 and tdc286, actinobacillus actinomycetemcomitans atcc29523 and jp2, fusobacterium nucleatum no. 2, tannerella forsythensis atcc43937, prevotella intermedia atcc25611 and streptococcus anginosus atcc33397, were treated with povidone-iodine (pvp-i) gargle (pvp-i: 0.47 and 0.23% w/v) or chlorhexidine gluconate (chg) gargle (chg: 0.002% w/v) for 15, 30 or 60 s, after which they were inoculated into various media, culture ...200616490986
effects of minocycline and doxycycline on cell survival and gene expression in human gingival and periodontal ligament cells.periodontitis is an infectious disease in the gingival crevice caused by periodontopathic bacteria, including porphyromonas gingivalis, prevotella intermedia, actinobacillus actinomycetemcomitans, and tennerella forsythensis, and antibacterial agents are directly administered to the site of infection to treat it. to maximize the therapeutic effects while reducing the adverse effects, the antibacterial agents should be administered at concentrations greater than their mic(90) doses required to in ...200616499715
detection of bacterial dna in atheromatous plaques by quantitative pcr.this is the first study to analyze atheromatous plaques for the presence of bacterial dna from ten species, including periodontal species and chlamydia pneumoniae. we examined 129 samples of dna extracted from atheromas from 29 individuals for the presence of bacterial 16s rdna sequences from ten different species: porphyromonas gingivalis, actinobacillus actinomycetemcomitans (a.a.), tannerella forsythensis, eikenella corrodens, prevotella intermedia, staphylococcus aureus, staphylococcus epide ...200616513386
relationship of serum advanced glycation end products with deterioration of periodontitis in type 2 diabetes patients.a close relationship between diabetes and chronic periodontitis has been demonstrated. we previously found that porphyromonas gingivalis with the type ii fima gene is an infectious factor closely associated with the deterioration seen in diabetic periodontitis patients. in the present study, we examined whether other biomarkers are related to the development and deterioration of periodontitis often seen in type 2 diabetic individuals.200616579698
local oxygen therapy for treating acute necrotizing periodontal disease in smokers.the main aim of treatment for acute necrotizing periodontal disease is fast and effective reduction of anaerobic destructive microorganisms to avoid periodontal damage. the effect of adjunctive local oxygen therapy in the treatment of necrotizing periodontal disease was examined in this study.200616579700
increase in probing depth is correlated with a higher number of prevotella intermedia genotypes.the aims of this study were to determine the genotypic diversity of prevotella intermedia in subgingival plaque samples by using two techniques, arbitrarily primed polymerase chain reaction (ap-pcr) and heteroduplex analysis, and to assess the relationship of this diversity with increase in probing depth.200616579704
efficiency of the vector -system compared with conventional subgingival debridement in vitro and in vivo.to assess the efficacy of the novel ultrasonic vector -system system for subgingival debridement and to compare the results with conventional periodontal instrumentation in vitro and in vivo.200616899100
identification of oral bacteria associated with crevicular epithelial cells from chronic periodontitis lesions.bacterial invasion of host epithelial cells plays an important role in the pathogenesis of periodontal diseases; however, the interactions between subgingival species and the gingival crevice cells are not fully understood. this study determined the prevalence of a group of oral bacterial species on or in epithelial cells derived from periodontal pockets and the gingival crevice of subjects with periodontitis. samples of epithelial cells were obtained from 120 sites with periodontal pockets > or ...200616585650
proteinase activity of prevotella species associated with oral purulent infection.prevotella intermedia and prevotella nigrescens are often regarded as principal causes of acute dentoalveolar infection; however, other species within the genus are also known to be associated with such infection. the aim of this study was to determine the in vitro proteolytic activity of these different prevotella species that have been implicated with dentoalveolar infection. a total of 234 strains were obtained from pus specimens from dentoalveolar infection and from the plaque of healthy vol ...200616586023
levels of matrix metalloproteinases-8 and -9 with simultaneous presence of periodontal pathogens in gingival crevicular fluid as well as matrix metalloproteinase-9 and cholesterol in blood.to investigate the levels of matrix metalloproteinase (mmp) -8 and -9 with the simultaneous presence of periodontal pathogens in gingival crevicular fluid (gcf) as well as mmp-9 and cholesterol in blood. although bacterial pathogens are required to initiate the periodontal disease process, in some individuals the reaction to bacteria may lead to an excessive host response, resulting in a general inflammatory response.200616953818
inhibitory effects of macrocarpals on the biological activity of porphyromonas gingivalis and other periodontopathic bacteria.macrocarpals, which are phloroglucinol derivatives contained in eucalyptus leaves, exhibit antimicrobial activity against a variety of bacteria including oral bacteria. this study examined effects of macrocarpals a, b, and c on periodontopathic bacteria, especially porphyromonas gingivalis.200616626372
role of mitogen-activated protein kinase kinase in porphyromonas gingivalis-induced myocardial cell hypertrophy and apoptosis.secreted factors present in the medium following growth of the periodontal pathogen porphyromonas gingivalis cause increased cardiomyocyte hypertrophy and apoptosis, whereas secreted factors from actinobacillus actinomycetemcomitans and prevotella intermedia have no such effects. the purpose of this study was to clarify the role of mitogen-activated protein kinase (mapk)/extracellular-regulated protein kinase (erk) pathways in p. gingivalis medium-induced h9c2 myocardial cell hypertrophy and apo ...200616630308
tlr4 mediates lps-induced vegf expression in odontoblasts.lipopolysaccharide (lps) from gram-negative bacteria cell walls such as prevotella intermedia and escherichia coli induce vascular endothelial growth factor (vegf) expression in odontoblasts, but not in undifferentiated dental pulp cells. cd14 and tlr4 are responsible for lps signaling in macrophages, but their expression levels and function in dental pulp cells are unknown. we showed here that murine odontoblast-like cells (mdpc-23) express cd14 and tlr4 by immunohistochemistry and flow cytomet ...200616982271
evaluation of peri-implant microbiota using the polymerase chain reaction in completely edentulous patients before and after placement of implant-supported prostheses submitted to immediate load.to evaluate, by means of the polymerase chain reaction (pcr), the presence of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia in the mandibular arch of completely edentulous subjects before implant placement and 4 and 6 months after the placement of mandibular implant-supported fixed prostheses.200616634497
supragingival plaque may modify the effects of subgingival bacteria on attachment loss.bacterial interactions in response to local and systemic factors may explain important features of the pathogenesis of periodontal disease. this study evaluates the role of supragingival plaque level on the relationship between subgingival microorganisms and the risk of attachment loss.200616671872
distribution of 10 periodontal bacteria in saliva samples from japanese children and their mothers.we analyzed the distribution of 10 periodontal bacteria species (porphyromonas gingivalis, tannerella forsythensis, prevotella intermedia, prevotella nigrescens, campylobacter rectus, eikenella corrodens, actinobacillus actinomycetemcomitans, capnocytophaga ochracea, capnocytophaga sputigena, and treponema denticola) in children, and then compared their distribution in those children and their mothers, with special attention given to three of the species known as the red complex (p. gingivalis, ...200616269128
synovial inflammation in active rheumatoid arthritis and psoriatic arthritis facilitates trapping of a variety of oral bacterial dnas.to investigate the presence of oral bacterial dnas in serum and synovial fluid (sf) of patients with active rheumatoid arthritis (ra) and psoriatic arthritis (psa).200617207381
association between passive smoking and salivary markers related to periodontitis.the mechanism of passive smoking in terms of development of periodontitis has not been investigated. this study examined the effect of passive smoking on salivary markers related to periodontitis.200616889628
java project on periodontal diseases. the natural development of periodontitis: risk factors, risk predictors and risk determinants.to identify risk factors, risk predictors and risk determinants for onset and progression of periodontitis.200616899096
microbiota of the dorsum of the tongue after plaque accumulation: an experimental study in humans.the purpose of the present investigation was to determine the effect of the absence of tongue hygiene on the microbiota of the dorsum of the tongue.200616945032
antibacterial effect of electrolyzed water on oral bacteria.this study investigated the antibacterial effect of electrolyzed water on oral bacteria both in vitro and in vivo. tap water was electrolyzed in a water vessel using platinum cell technology. the electrolyzed tap water (called puri-water) was put in contact with five major periodontopathogens or toothbrushes contaminated with these bacteria for 30 sec. in addition, puri-water was used as a mouthwash for 30 sec in 16 subjects and the antibacterial effect on salivary bacteria was evaluated. puri-w ...200616953177
periodontal therapy in siblings with papillon-lefèvre syndrome and tinea capitis: a report of two cases.report of clinical and microbiological periodontal findings before and 6 months after treatment of two siblings with papillon-lefèvre syndrome (pls) and tinea capitis.200616970621
changes in the subgingival microbiota and periodontal parameters before and 3 months after bracket placement.dental plaque is organized in a biofilm complex that provides protection and nutrients for periodontopathic bacteria. several factors can affect microbial colonization, including restorations and orthodontic brackets. the aim of this study was to investigate changes in subgingival microbiota and clinical parameters before and after bracket placement.200616979483
photodynamic therapy for endodontic disinfection.the aims of this study were to investigate the effects of photodynamic therapy (pdt) on endodontic pathogens in planktonic phase as well as on enterococcus faecalis biofilms in experimentally infected root canals of extracted teeth. strains of microorganisms were sensitized with methylene blue (25 microg/ml) for 5 minutes followed by exposure to red light of 665 nm with an energy fluence of 30 j/cm2. methylene blue fully eliminated all bacterial species with the exception of e. faecalis (53% kil ...200616982278
in vitro evaluation of the antimicrobial activity of calcium hydroxide combined with chlorhexidine gel used as intracanal medicament.the aim of this study was to investigate the antimicrobial activity of calcium hydroxide (ca(oh)2) combined with 2% chlorhexidine gluconate (chx) gel against endodontic pathogens and to compare the results with the ones achieved by ca(oh)2 mixed with sterile water and by chx gel alone. two methods were used: the agar diffusion test and the direct contact test. ca(oh)2 + 2% chx gel produced inhibitory zones ranging from 2.84 to 6.5 mm, and required from 30 seconds to 6 hours to eliminate all test ...200616997123
in vitro antimicrobial activity of sodium hypochlorite and chlorhexidine against selected single-species biofilms.to investigate the antimicrobial activity of 2.5% and 5.25% sodium hypochlorite and 2.0% chlorhexidine gel and liquid as endodontic-irrigating substances against selected single-species biofilms.200617014526
proinflammatory and antimicrobial nitric oxide in gingival fluid of diabetic patients with periodontal disease.abnormal nitric oxide (no) synthesis has been implicated in the pathogenesis of both periodontal disease and diabetes mellitus. in diabetic patients, increased inducible no synthase in inflamed gingiva correlated with no in gingival crevicular fluid. although increased no reflected more-severe inflammation, it was associated with reductions in cfu of prevotella intermedia, a major periodontopathogen, highlighting dual roles for no.200617015454
denaturing gradient gel electrophoresis as a diagnostic tool in periodontal microbiology.bacteria play an important role in the initiation and progression of periodontal diseases and are part of a biofilm, which can contain over 100 different species. the aim of the present study was to show the potential of denaturing gradient gel electrophoresis (dgge) as a tool for the detection of clinically relevant species and to compare the results of detection by dgge with those by pcr and culturing. hybridization of the bands from the dgge profiles with species-specific probes was developed ...200617021091
identification and characterization of a cell surface protein of prevotella intermedia 17 with broad-spectrum binding activity for extracellular matrix proteins.prevotella intermedia binds and invades a variety of host cells. this binding is most probably mediated through cell surface proteins termed adhesins. to identify proteins binding to the host extracellular matrix (ecm) component, fibronectin, and study the molecular mechanism underlying bacterial colonization, we applied proteomic approaches to perform a global investigation of p. intermedia strain 17 outer membrane proteins. 2-de followed by far western blot analysis using fibronectin as a prob ...200617051640
carpegen real-time polymerase chain reaction vs. anaerobic culture for periodontal pathogen identification.the aim of this study was to compare two methods of microbiological diagnosis, anaerobic bacterial culture and real-time polymerase chain reaction (pcr), for the detection of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, tannerella forsythia, fusobacterium nucleatum, and treponema denticola.200617064390
the effects of tetracycline, minocycline, doxycycline and ofloxacin on prevotella intermedia biofilm.prevotella intermedia, a black-pigmented, anaerobic, gram-negative bacterium, is associated with various type of periodontitis. antibiotic treatments via a systemic or local route have been reported as being useful for treating periodontal disease. the purpose of this study was to examine the effects of four antibiotics, tetracycline (tet), minocycline (mino), doxycycline (doxy) and ofloxacin (oflx) on p. intermedia biofilms at minimum inhibitory concentrations (mic) from one-fold to 100-fold. m ...200617064394
transcriptional organization, regulation and role of the porphyromonas gingivalis w83 hmu haemin-uptake locus.porphyromonas gingivalis, an oral bacterium associated with periodontal disease, requires haemin for growth. although several multigenic clusters encoding haemin-uptake systems are present on the genome of p. gingivalis, little is known regarding their transcriptional organization and expression. this study identified a 23 kda iron-regulated haemin-binding protein encoded by a larger than previously reported variant of hmuy. it was shown that the hmu locus is larger than previously reported and ...200617074906
cytokine production in human periodontal ligament cells stimulated with porphyromonas gingivalis.although some functions and characterizations of human periodontal ligament (hpdl) cells have been reported, the role of hpdl cells in periodontal disease is poorly understood. we have previously reported that hpdl cells produce many kinds of inflammatory cytokines by stimulation with prevotella intermedia. in this study, we examined the production of cytokines in hpdl cells stimulated with porphyromonas gingivalis as compared with p. intermedia.200617076781
degradation of human hemoglobin by prevotella intermedia.in this study, the ability of prevotella intermedia, an obligate anaerobic rod, to degrade human hemoglobin was determined by sds-page and the degradation was quantified by scanning densitometry. both bacterial cells and culture supernatants degraded hemoglobin. the hemoglobin degradation by p. intermedia was time-dependent, heat sensitive, ph related and was not influenced by iron restriction. inhibition studies demonstrated that a cysteine protease might be involved in hemoglobin degradation a ...200617081784
effects of sonicated prevotella intermedia, fusobacterium nucleatum and lactobacillus casei extracts on interleukin-8 production by human dental pulp cells.the objective of this study was to determine the effects of prevotella intermedia, fusobacterium nucleatum and lactobacillus casei on the production of il-8 by human dental pulp cells. human dental pulp cells from teeth of young patients (aged 18-25 years) were cultured and tested with sonicated p. intermedia atcc 25611, f. nucleatum atcc 25586 and l. casei atcc 4646 extracts. il-8 secreted into the culture supernatants were measured at 6, 12 and 24 hours using a quantitative sandwich enzyme imm ...200617120973
[streptococcus constellatusas a causative agent of empyema. report of one case].streptococcus constellatus is a commensal microorganism in man but may cause infections in different locations. we report a 59 years old male with severe sequelae of a previous cerebrovascular accident that consulted in the emergency room for fever of 15 days of evolution. a right empyema was diagnosed. the bacteriological culture of the effusion disclosed the presence of streptococcus constellatus and two anaerobic strains (prevotella intermedia and fusobacterium urealyticus). the patient was t ...200617130992
effects of periodontal therapy during pregnancy on periodontal status, biologic parameters, and pregnancy outcomes: a pilot study.few studies have examined the potential effects of periodontal treatment during pregnancy on pregnancy outcomes, periodontal status, and inflammatory biomarkers.200617209786
use of dna probes in the diagnosis and treatment of periodontitis --a case series.aggressive periodontitis is characterized by rapid attachment and bone loss with no underlying systemic disease and is associated with specific bacteria like actinobacillus actinomycetemcomitans (aa) and porphyromonas gingivalis (pg). in this case series 25 patients were diagnosed with aggressive periodontitis by the aid of dna probes for aa and pg and other periodontal pathogens. the use of dna probes for the detection of periodontal pathogens may aid in the diagnosis and treatment of aggressiv ...200617243578
[dissertations 25 years after date 12. classification and virulence of black-pigmented bacteria in relation to periodontitis].bacteria in dental plaque play an essential role in the origin and development of periodontitis. in the seventies of the last century it became clear that black-pigmented bacteria of the genus bacteroides play a vital role in this process. these bacteria are currently known as porphyromonas gingivalis and prevotella intermedia. in a phd dissertation 25 years ago it was shown by dna analysis that this group of bacteria is very heterogeneous, and that different species exist, which are associated ...200617193988
evaluation of subgingival bacteria in the dog and susceptibility to commonly used antibiotics.the aim of the present investigation was to evaluate the subgingival aerobic and anaerobic flora of 13 dogs with periodontal disease and the susceptibility of these bacteria to antibiotics currently approved in italy for treatment of canine infections. of the anaerobic bacteria, bacteroides fragilis was most frequently isolated, followed by peptostreptococcus + porphyromonas gingivalis and prevotella intermedia. of the aerobic bacteria, alpha-hemolytic streptococcus was most frequently isolated, ...200617286127
identification of the microorganisms responsible for periodontopathy by multiplex rt-pcr.the aim of our research was to identify by bacterial genomic dna analysis the prevalence of five different species of periodontopathogenic bacteria present in the subgingival biofilm, specifically: actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), bacterioides forsytus (bf), treponema denticola (td). for the analysis we used the systematic multiplex-pcr-microdent kit with species-specific primers. we studied a group of 48 subjects, 18 males and ...200617263160
prospective study of the long term change of the oral flora after radiation therapy.the aim of this prospective study was to evaluate the long term change in oral pathogens following radiation therapy.200616341728
the ability of selected oral microorganisms to emit red fluorescence.some novel caries detection and excavation devices rely on the ability of bacteria to produce red fluorescing compounds. the aim of this study was to examine the ability of selected oral microorganisms to emit red fluorescence. streptococcus mutans, s. oralis, s. salivarius, s. sobrinus, lactobacillus fermentans, l. casei, l. rhamnosus, actinomyces naeslundi, a. israelii, prevotella intermedia, and fusobacterium nucleatum were inoculated onto columbia agar with haemin and vitamin k and incubated ...200616352873
immediate effect of instrumentation on the subgingival microflora in deep inflamed pockets under strict plaque control.to investigate (1) reduction in the number of microorganisms obtained directly after subgingival instrumentation, (2) rate of bacterial re-colonization during 2 weeks, under supragingival plaque-free conditions.200616367855
the additional value of real-time pcr in the quantitative detection of periodontal pathogens.for the analysis of subgingival plaque, anaerobic bacterial culture has been the gold standard for many years. currently, molecular microbial techniques have become available to identify and quantify target organisms with high specificity and sensitivity. the technique of real-time polymerase chain reaction(rt-pcr) provides a new tool to detect oral pathogens both in oral and non-oral human infections. the aim of this study was to compare the rt-pcr and anaerobic culture for detection and quanti ...200616677332
role of calcineurin in porphyromonas gingivalis-induced myocardial cell hypertrophy and apoptosis.periodontal pathogen porphyromonas gingivalis (p. gingivalis) increased cardiomyocyte hypertrophy and apoptosis whereas actinobaeillus actinomycetemcomitans and prevotella intermedia had no effects. the purpose of this study is to clarify the role of calcineurin signaling pathway in p. gingivalis-induced h9c2 myocardial cell hypertrophy and apoptosis.200616369686
relationship of periodontal bacteria and porphyromonas gingivalis fima variations with phenytoin-induced gingival overgrowth.we investigated the relationship between phenytoin-induced gingival overgrowth (go) and the harboring of periodontal bacteria.200616390469
porphyromonas gingivalis heat shock protein vaccine reduces the alveolar bone loss induced by multiple periodontopathogenic bacteria.heat shock protein (hsp) can be utilized as a vaccine to cross-protect against multiple pathogenic species. the present study was performed to evaluate porphyromonas gingivalis heat shock protein 60 (hsp60) as a vaccine candidate to inhibit multiple bacteria-induced alveolar bone loss.200616409250
antimicrobial actions of benzimidazoles against the oral anaerobes fusobacterium nucleatum and prevotella intermedia.benzimidazoles are widely used as proton-pump inhibitors to control stomach hyperacidity and have been found also to have antimicrobial actions against helicobacter pylori and oral streptococci. our primary aim was to determine if they are active also against oral anaerobes associated with gingivitis. our major focus was on catabolism because it leads to production of inflammatory metabolites such as butyrate and ammonia. the benzimidazoles are effective in the protonated form at acid ph values ...200616806047
multiplex polymerase chain reaction detection of black-pigmented bacteria in infections of endodontic origin.the purpose of this study was to detect the presence of porphyromonas endodontalis, p. gingivalis, prevotella intermedia, p. nigrescens, and p. tannerae from clinical samples using multiplex polymerase chain reactions (pcr). two different multiplex pcr protocols were used (one for the two porphyromonas species and the other for the three prevotella species), each one using a primer pair specific for each target species. the results were compared to those of the conventional culture procedures. m ...200616427456
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