Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| down regulation by p60v-src of genes specifically expressed and developmentally regulated in postmitotic quail neuroretina cells. | the avian neuroretina (nr) is composed of photoreceptors and different neurons that are derived from proliferating precursor cells. neuronal differentiation takes place after terminal mitosis. we have previously shown that differentiating nr cells can be induced to proliferate by infection with rous sarcoma virus (rsv) and that cell multiplication requires expression of a functional v-src gene. we speculated that the quiescence of nr cells could be determined by specific genes. cell proliferatio ... | 1990 | 2162475 |
| mediation of wound-related rous sarcoma virus tumorigenesis by tgf-beta. | in rous sarcoma virus (rsv)-infected chickens, wounding leads to tumor formation with nearly 100% frequency in tissues that would otherwise remain tumor-free. identifying molecular mediators of this phenomenon should yield important clues to the mechanisms involved in rsv tumorigenesis. immunohistochemical staining showed that tgf-beta is present locally shortly after wounding, but not unwounded controls. in addition, subcutaneous administration of recombinant transforming growth factor-beta 1 ( ... | 1990 | 2163544 |
| potent growth inhibition of human tumor cells in culture by arginine deiminase purified from a culture medium of a mycoplasma-infected cell line. | two kinds of growth-inhibitory substances were found in culture of a rous sarcoma virus-transformed rat liver cell line, rsv-brl. the two substances were purified from the serum-free culture medium and identified as transforming growth factor beta 1 and mycoplasma-derived arginine deiminase (ec 3.5.3.6), respectively. the arginine deiminase was an acid-labile but dithiothreitol-resistant protein with a molecular weight of 45,000 and pi 4.7. its km value for l-arginine was 0.3 mm, which is about ... | 1990 | 2164440 |
| transformation-defective mutants with 5' deletions of the src gene are frequently generated during replication of rous sarcoma virus in established quail fibroblasts. | replication of rous sarcoma virus (rsv) in avian fibroblasts leads to the generation of replication-competent variants that are defective for cell transformation (td virus). these td variants contain deletions affecting various portions of the v-src gene. we compared the rate of td virus production in q3b cells, a quail cell line established by mutagen treatment, and in normal quail fibroblasts. twenty-five days after infection with an rsv stock containing only transforming virions, q3b cells ha ... | 1990 | 2164723 |
| a group level analysis of the associations between antibodies to seven putative pathogens and respiratory disease and weight gain in ontario feedlot calves. | the associations, at the group level, between serological titer to pasteurella haemolytica surface antigens (ph), pasteurella haemolytica cytotoxin (ph-cytox), infectious bovine rhinotracheitis virus (ibrv), bovine virus diarrhea virus (bvdv), parainfluenza-3 virus (piv3), respiratory syncytial virus (rsv), mycoplasma dispar (md), m. bovis (mb), and respiratory disease treatment rates, relapse rates, and 28 day weight gains were investigated in 14 groups of calves entering two feedlots during ye ... | 1990 | 2165846 |
| fusion of rous-sarcoma-virus-transformed rat cells to morphologically normal human or rat cells results in transcriptional suppression of the provirus that depends on its chromosomal integration site. | the fusion of a rous sarcoma virus (rsv)-transformed rat fibroblast clone to at least 2 different human cell types reproducibly produces phenotypically normal hybrids. analysis of such hybrids reveals that proviral silence is the result of transcriptional down-regulation, presumably by a trans-acting human molecule. furthermore, this phenomenon seems to be strongly influenced by the proviral chromosomal integration site and its imposition may entail a mechanism that is required only transiently. | 1990 | 2166711 |
| incorporation of chimeric gag protein into retroviral particles. | the product of the rous sarcoma virus (rsv) gag gene, pr76gag, is a polyprotein precursor which is cleaved by the viral protease to yield the major structural proteins of the virion during particle assembly in avian host cells. we have recently shown that myristylated forms of the rsv gag protein can induce particle formation with very high efficiency when expressed in mammalian cells (j. w. wills, r. c. craven, and j. a. achacoso, j. virol. 63:4331-4343, 1989). we made use of this mammalian sys ... | 1990 | 2166812 |
| pp60v-src association with the cytoskeleton induces actin reorganization without affecting polymerization status. | the mechanism by which rous sarcoma virus (rsv) induces a reorganization of actin and its associated proteins and a reduction in microfilament bundles is at present poorly understood. to examine the relationship between the organization of the microfilament system and the polymerization state of actin after transformation, we have investigated these changes in a rat-1 cell line transformed by la29, a temperature-sensitive (ts) mutant of rsv. parallel immunofluorescence and biochemical analysis d ... | 1990 | 2167224 |
| morphological aspects of inhibition of transformation of rat kidney cells infected with a temperature-sensitive mutant of the rous sarcoma virus by rat fibroblast interferon. | normal rat kidney cells (nrk) infected with a temperature-sensitive (ts) mutant of rous sarcoma virus (rsv) at the permissive temperature (33 degrees c) exhibit the morphological and growth characteristics of the transformed state. by the use of this system, it has been shown that the addition of rat fibroblast interferon to infected cells grown at a nonpermissive temperature (38 degrees c) prevents re-transformation when they are introduced to the permissive temperature. merely treating cells a ... | 1990 | 2168311 |
| the effects of 3-deazaguanine on rous sarcoma virus transformation. | an expression of cellular transformation in chicken embryo fibroblast infected with rous sarcoma virus (rsv) was inhibited by 3-deazaguanine (3-dg). re-transformation of normal rat kidney cells infected with a temperature-sensitive mutant of rsv was suppressed by 3-dg when the cells were shifted from the non-permissive temperature (38 degrees c) to the permissive temperature (33 degrees c). removal of the inhibitor enabled the cells to revert to the transformed state. finally, 3-dg had little or ... | 1990 | 2168314 |
| fusion of rous sarcoma virus with host cells does not require exposure to low ph. | we investigated whether rous sarcoma virus (rsv) infects cells through a ph-independent or a low-ph-dependent pathway. to do this, the effects of lysosomotropic agents and acid pretreatment on rsv infectivity of, and fusion with, chicken embryo fibroblasts (cefs) were studied. high concentrations of lysosomotropic agents (ammonium chloride and monensin) did not inhibit virus infectivity: equal titers of rsv were produced in the presence and absence of these agents. similarly, low-ph pretreatment ... | 1990 | 2168989 |
| [antiviral and interferon-inducing effect of fang-gan mixture]. | the present study was carried out by using the plaque formation test, microdosage cytopathic effect (cpe) method, and mice nasal-cavity-attacking method to observe the antiviral and interferon-inducing effect of fang-gan mixture (fgm). the results showed that fgm has direct respiratory syncytia virus (rsv) inactivation effect, and protection effect after mice infected by rsv. fgm could also decrease mice death rate (p less than 0.01) in the experimentation of using parainfluenza virus to attack ... | 1990 | 2169355 |
| malignant transformation of human endometrial stromal cells by transfection of c-myc: effects of prsvneo cotransfection and treatment with mnng. | stromal cells isolated from normal human endometrium were cotransfected in primary culture with psvc-myc, a plasmid containing a truncated c-myc gene regulated by simian virus 40 promoter, and prsv neo, a plasmid containing a neomycin resistance gene regulated by rous sarcoma virus (rsv) promoter. these cells demonstrated properties of transformed cells in vitro, including altered morphology, focus formation, anchorage-independent growth, chromosomal alterations, and tumor formation in athymic m ... | 1990 | 2171549 |
| transactivation of the rous sarcoma virus long terminal repeat promoter by marek's disease virus. | transient expression of chloramphenicol acetyltransferase (cat) was used to study marek's diseases virus (mdv)-mediated transactivation of the rous sarcoma virus long terminal repeat (rsv-ltr) promoter. cotransfection experiments in primary avian cells were conducted using mdv high-molecular-weight dna and plasmid prsvcat. increased cat activity, relative to controls, was consistently observed in the presence of mdv. enhanced cat activity, expressed via the rsv-ltr promoter, was strictly depende ... | 1990 | 2173259 |
| sequence specificity of mrna n6-adenosine methyltransferase. | the sequence specificity of chicken mrna n6-adenosine methyltransferase has been investigated in vivo. localization of six new n6-methyladenosine sites on rous sarcoma virus (rsv) virion rna has confirmed our extended consensus sequence for methylation: rgacu, where r is usually a g (7/12). we have also observed a (2/12) and u (3/12) at the -2 position (relative to m6a at +1) but never a c. at the +3 position, the u was observed 10/12 times; an a and a c were observed once each in weakly methyla ... | 1990 | 2173695 |
| a retroviral promoter is sufficient to convert proto-src to a transforming gene that is distinct from the src gene of rous sarcoma virus. | the src genes of four natural isolates of avian sarcoma viruses differ from cellular proto-src in two genetic substitutions: the promoter of the cellular gene is replaced by a retroviral counterpart, and at least six codons from the 3' terminus are replaced by retroviral or heterologous cell-derived elements. since virus constructs with a complete proto-src coding region failed to transform avian cells but acquired transforming function by point mutations of various codons, it has been proposed ... | 1990 | 2174554 |
| isolation and identification of viral agents in argentinian children with acute lower respiratory tract infection. | from a total population of 1,002 children with acute lower respiratory infection (alri), identification of virus was achieved in 304 cases. in this survey, 1,000 nasopharyngeal aspirate and 13 lung tissue samples were tested in four cell lines (hep-2, mrc-5, mdck, and llc-mk2) and by indirect immunofluorescence (iif), while 242 paired sera were studied by complement fixation. respiratory syncytial virus (rsv) was the most frequently detected agent (n = 183), followed by adenovirus (n = 28), para ... | 1990 | 2176736 |
| acute lower respiratory tract infection due to virus among hospitalized children in dhaka, bangladesh. | the nature of acute lower respiratory tract infection (alri) in hospitalized children and of the associated viral agents was assessed in a study of 601 children less than 5 years old over a 24-month period. of these children, 80% were less than 24 months of age and the ratio of boys to girls was 1.7:1. pneumonia (86.5% of cases) was the most frequently observed clinical manifestation. shedding of virus was detected in 21.1% of the children; the highest rate occurred in infants 0-5 months old (27 ... | 1990 | 2176737 |
| regulation of integrin mobility and cytoskeletal association in normal and rsv-transformed chick embryo fibroblasts. | the mobility of the integrin receptor in trypsinised chick embryo fibroblasts (cef) was investigated using the csat monoclonal antibody. the binding of csat to trypsinised cef followed by incubation at 37 degrees c resulted in patching and then capping of the receptor. this capping was dependent on cellular metabolism, since agents such as sodium azide or 2-deoxyglucose inhibited the process. whereas about 95% of unclustered integrin was soluble in nonidet p40-containing buffers, after capping m ... | 1990 | 2177479 |
| an epidemic of respiratory syncytial virus in elderly people: clinical and serological findings. | in 1984-1985, an outbreak of respiratory syncytial virus (rsv) infection occurred in two geriatric wards. among 68 patients (mean age +/- sd = 82.5 +/- 12.5 with respiratory signs, 52 had signs caused by rsv infection. among all patients, the clinical and serological attack rates were 61.2% and 75.0%, respectively. the most frequent clinical presentation was intensive coughing (96.1%) and fever (96.1%) associated with expectorate (63.5%). the duration of the respiratory symptoms was 5 to 7 days. ... | 1990 | 2179469 |
| [once more a discussion of the rsv affair]. | during autumn- and winter epidemics respiratory syncytial (rs) virus accounts for the majority of respiratory infections in infants and young children. in case of an acute lower respiratory tract infection, rs virus can induce serious symptoms. these are age-dependent. the most important symptoms in babies and toddlers are dyspnea, wheezing, cyanosis and apneas. in the case of respiratory insufficiency or fatigue, as well as recurrent apneas, mechanical ventilation is required. diagnosis can be ... | 1990 | 2180118 |
| comparison of directigen rsv with viral isolation and direct immunofluorescence for the identification of respiratory syncytial virus. | an enzyme immunoassay membrane test (directigen rsv) for the detection of respiratory syncytial virus in clinical specimens was compared prospectively with isolation in cell culture and direct immunofluorescence (if). a total of 315 nasopharyngeal wash specimens from pediatric patients were examined. directigen rsv was 86.1% sensitive and 91.3% specific for specimens positive by isolation in cell culture and/or if, with 88.6% agreement. the false-positive rate was 16%; 2 of 20 specimens giving f ... | 1990 | 2182668 |
| evaluation of five methods for respiratory syncytial virus detection. | a total of 117 nasal aspirates were cultured for respiratory syncytial virus (rsv) and tested for rsv antigen by a direct fluorescent-antibody (dfa) test (bartels immunodiagnostic supplies, inc., bellevue, wash.), the directigen enzyme immunoassay (eia; becton dickinson microbiology systems, cockeysville, md.), the testpack eia (abbott laboratories, north chicago, ill.), and rsv eia (abbott). agreement of two of five methods or a positive rsv culture were required to validate a result. a total o ... | 1990 | 2191003 |
| immunoprophylaxis of infections with respiratory syncytial virus: observations and hypothesis. | infections with respiratory syncytial virus (rsv) may cause substantial morbidity and increased mortality in neonates and infants, especially those born before term and/or with pulmonary or cardiac disease. humoral immunity was long thought to play only a small role in prevention, reduction of severity, or recovery from rsv infections of the lower respiratory tract. we recently performed a series of experiments in rsv-infected cotton rats, owl monkeys, and human infants to assess the role of hum ... | 1990 | 2194271 |
| a rapid test for detection of respiratory syncytial virus in nasopharyngeal secretion. | a new rapid membrane enzyme immunoassay (meia; directigen rsv) for detection of respiratory syncytial virus (rsv) was evaluated using samples of nasopharyngeal secretion from infants and children with acute respiratory disease. the meia was compared with an immunofluorescent antibody (if) technique using a sensitive biotin-avidin (ba) eia as reference. of 242 samples tested, 108 were positive by the meia and 123 by the ba-eia. of 144 samples which were also tested by the if technique, 57 were po ... | 1990 | 2197094 |
| what is the clinical role of aerosolized ribavirin? | aerosolized ribavirin is the first approved agent for the treatment of respiratory syncytial virus (rsv) infection. however, the clinical use of ribavirin is controversial. although ribavirin-treated patients appear to be subjectively improved, the benefit in terms of decreased morbidity and mortality is not well established. furthermore, the drug has been found to be teratogenic, carcinogenic, and mutagenic, depending upon the scientific model. because of its unique mode of administration, envi ... | 1990 | 2197815 |
| rapid detection of respiratory syncytial virus and influenza a virus in cell cultures by immunoperoxidase staining with monoclonal antibodies. | peroxidase-labeled monoclonal antibodies against respiratory syncytial virus (rsv) and influenza a virus were used for immunoperoxidase staining (ips) of cell cultures inoculated with nasopharyngeal aspirates. cells were grown in 24-well plates, and specimens were inoculated by low-speed centrifugation. cultures were incubated for 2 days at 37 degrees c and then fixed, stained, and observed by light microscopy. ips was compared with standard virus isolation by using cultures of human diploid fib ... | 1990 | 2199488 |
| detection of respiratory syncytial virus antigen in nasal washings by abbott testpack enzyme immunoassay. | we compared the new abbott testpack (tp) respiratory syncytial virus (rsv) enzyme immunoassay (eia) with cell culture and two commercial rsv eias (from abbott diagnostics and kallestad laboratories) by using split samples of fresh nasal washings from children with suspected rsv disease. two tubes of hep-2 cells were inoculated and observed for cytopathic effect for 14 days, and isolates were confirmed by immunofluorescence. the tp eia was performed by following the manufacturer's instructions. s ... | 1990 | 2199500 |
| detection of respiratory syncytial virus antigen after seventy-two hours of culture. | between september, 1987, and april, 1989, three techniques for the detection of respiratory syncytial virus (rsv) were compared: indirect immunofluorescence (if) on the sample, indirect immunofluorescence after 72 h of mrc-5 cell culture (if 72h), and detection of the cytopathic effect (cpe) by mrc-5 and hep-2 cell culture. a study of 383 nasal aspirates from young children admitted to the centre hospitalier de nantes (chr) showing miscellaneous respiratory symptoms produced the following result ... | 1990 | 2202784 |
| comparison of immunofluorescence & culture for the diagnosis of respiratory syncytial virus infection. | in 809 infants and children with acute respiratory infection, hep-2 cells were used for the isolation of respiratory syncytial virus (rsv) and an indirect immunofluorescence technique (iif) was used for the detection of rsv antigen in the epithelial cells of nasopharyngeal secretions. while rsv was detected in culture in only 87 subjects, iif was positive for viral antigen in 158 subjects. in children with bronchiolitis and in those with pneumonia 57 and 19 per cent respectively, had evidence of ... | 1990 | 2228050 |
| occurrence of groups a and b of respiratory syncytial virus over 15 years: associated epidemiologic and clinical characteristics in hospitalized and ambulatory children. | over 15 years respiratory syncytial virus (rsv) isolates from 1209 hospitalized and ambulatory children were examined for strain group and in a subset for subgroup to determine the associated epidemiologic and clinical characteristics. three patterns of yearly outbreaks existed: (1) strong predominance of group a strains (9 years with 83%-100% a strains), (2) relatively equal proportions of group a and b strains (4 years), and (3) strong predominance of group b strains (78%-85%) in 2 years, sepa ... | 1990 | 2230258 |
| rapid recovery in mice after combined nasal/oral immunization with killed respiratory syncytial virus. | based on the concept of a common mucosal immune system, the murine gastrointestinal tract was inoculated (oral) with three doses (5, 20, and 40 micrograms) of uv-inactivated respiratory syncytial virus (rsv) in order to elicit a virus-specific immune response in the respiratory tract. only the 40 micrograms dose induced significant (p less than 0.01) anti-rsv-igg rises in serum and lung wash compared to controls. to improve the immune response, mice were immunized intranasally under light anesth ... | 1990 | 2243208 |
| does prematurity alter the course of respiratory syncytial virus infection? | we compared previously healthy prematurely born infants with full-term infants hospitalized with respiratory syncytial virus (rsv) infection to evaluate the role of prematurity on the clinical course of the illness. during a 5-yr period (1984 to 1989), 484 previously healthy patients were admitted to the hospital with rsv infection. no differences were found in the presenting symptoms of respiratory distress, cough, fever or shock, although the premature group was more likely to present with apn ... | 1990 | 2245609 |
| enhanced pulmonary histopathology is observed in cotton rats immunized with formalin-inactivated respiratory syncytial virus (rsv) or purified f glycoprotein and challenged with rsv 3-6 months after immunization. | formalin-inactivated (fi) rsv, purified f glycoprotein in alum, and rsv infection (intranasal) were compared for their immunogenicity, efficacy, and ability to enhance pulmonary histopathology during rsv infection 3 and 6 months following immunization by the intramuscular route. purified influenza virus in alum was used as a control immunogen. at 1 month following immunization with one dose of purified f glycoprotein (5 micrograms), cotton rats developed levels of f antibodies (elisa) higher tha ... | 1990 | 2251875 |
| evaluation of direct immunofluorescence, enzyme immunoassay, centrifugation culture, and conventional culture for the detection of respiratory syncytial virus. | four methods of detecting respiratory syncytial virus (rsv) from clinical specimens were evaluated. a total of 410 specimens consisting of nasopharyngeal washes, aspirates, and swabs were simultaneously tested for the presence of rsv by direct immunofluorescence assay (dfa), enzyme immunoassay (eia) (kallestad pathfinder), shell vial centrifugation culture (svc), and conventional culture. dfa identified 146 (83%) of the 175 positive cases, eia detected 153 (87%), svc detected 127 (73%), and conv ... | 1990 | 2254415 |
| virus-induced alterations in macrophage production of tumor necrosis factor and prostaglandin e2. | the cellular immune response to respiratory syncytial virus (rsv) is felt to contribute to viral clearance and/or the inflammation accompanying pulmonary infections with this virus. both tumor necrosis factor (tnf) and prostaglandin e2 (pge2) are important regulatory mediators of the cellular immune response. we examined the production of these mediators from purified human alveolar and blood mononuclear phagocytes (mp) after rsv infection in vitro and compared production induced by virus with t ... | 1990 | 2260674 |
| identification of viruses in a study of acute respiratory tract infection in children from uruguay. | for the purpose of identifying viral agents associated with acute respiratory tract infections (ari) in children less than 5 years old, a longitudinal community study was undertaken in montevideo, uruguay, from may 1985 to december 1987. this report includes results obtained by cell culture and immunofluorescence techniques for detection of respiratory syncytial virus (rsv), influenza a and b viruses, parainfluenza 1 and 3 viruses, and adenovirus. two populations were studied: children visited a ... | 1990 | 2270420 |
| complementarity between the 5'- and 3'-terminal sequences of rice stripe virus rnas. | the 5' and 3' termini of four ssrna species of rice stripe virus (rsv) isolate t were sequenced. the 3' termini of the three smallest ssrnas, i.e. rnas 2, 3 and 4, had the sequence 5' gacuuugugu 3'; that of ssrna 1 had the sequence 5' gacuaugugu 3'. the 5'-terminal sequences of all four ssrnas were 5' acacaaagucc 3'. the 5'- and 3'-terminal sequences of about 20 bases of each ssrna were almost complementary to each other. it is possible that rsv rnas form panhandle structures characteristic of t ... | 1990 | 2273384 |
| the two open reading frames of the 22k mrna of human respiratory syncytial virus: sequence comparison of antigenic subgroups a and b and expression in vitro. | the sequence of the 22k mrna of strain 18537 of antigenic subgroup b of human respiratory syncytial virus (rsv) was determined by sequencing cloned cdnas of intracellular mrna. comparison with the corresponding sequence of the a2 strain of subgroup a showed that there is 78% nucleotide sequence identity overall, that the amino acid sequence of the 22k protein is 92% identical between subgroups and that the 22k mrna of both subgroups contains a second, internal, overlapping open reading frame (or ... | 1990 | 2273393 |
| [serologic response measured by complement fixation in children with acute respiratory infection]. | the aim of this work was to evaluate the sensitivity of complement fixation for serological diagnosis of lower acute respiratory infections (ari) in small children in comparison with direct methods such as indirect immunofluorescence (iif) on nasopharyngeal aspirate and tissue culture isolation. sera from children under 5 year of age with ari were studied by complement fixation for 6 respiratory viruses (respiratory syncytial virus (rsv), adenovirus, influenza a and b and parainfluenza 1 and 3). ... | 1990 | 2287713 |
| serodiagnosis of respiratory syncytial virus (rsv) infection in children as measured by detection of rsv-specific immunoglobulins g, m, and a with enzyme-linked immunosorbent assay. | the diagnostic value of an enzyme-linked immunosorbent assay for detection of respiratory syncytial virus (rsv)-specific immunoglobulin g (igg), igm, and iga in sera from infants and children with proven rsv infection, from a control group, and from patients with symptoms of viral respiratory disease was analyzed. compared to virus isolation and rsv antigen detection methods, the sensitivity of this assay was 87% and the specificity was 79%. for igg alone, these were 45 and 92%, for igm alone th ... | 1990 | 2298874 |
| severe respiratory syncytial virus infection in older children. | serious respiratory syncytial virus (rsv) disease requiring hospitalization occurs primarily in infants younger than 12 months. the incidence, risk factors, and clinical features in older children have not been studied extensively. of 282 children hospitalized at our institution with severe rsv disease during a 3-year period, 62 (22%) were older than 12 months. these 62 older children were matched for sex, onset of illness, and hospital location with 62 hospitalized children younger than 12 mont ... | 1990 | 2305743 |
| refractory congestive heart failure after ribavirin in infants with heart disease and respiratory syncytial virus. | although treatment with ribavirin has been known to be associated with a decreased mortality in infants with congenital heart disease (chd) who have respiratory syncytial virus (rsv), few data are available regarding morbidity. we reviewed records of 10 consecutively hospitalized infants with chd during a recent rsv epidemic. despite the presence of left-to-right shunt in each patient, symptoms of rsv were respiratory at presentation. after ribavirin, decreased respiratory symptoms were found in ... | 1990 | 2308658 |
| a seroepidemiologic study of bovine respiratory syncytial virus (brsv) in turkey. | in this study, a survey of cattle for neutralizing antibodies to respiratory syncytial virus (rsv) in turkey is presented. 490 serum samples were collected from several state farms and some private flocks in different regions of turkey. the serologic examination was done by the microtiter serum-neutralization technique. in this report, a positive serum antibody titer at dilutions of 1:2 was demonstrated in 226 (46.12%) of the 490 sera tested. | 1990 | 2311535 |
| viral aetiology of acute respiratory infections in children in north india. | two hundred and thirty children clinically diagnosed as suffering from acute respiratory infection were tested for four major groups of viral aetiological agents, i.e. influenza para-influenza, respiratory syncytial virus (rsv) and adenoviruses using indirect immunofluorescence technique. at least one of the respiratory viruses was identified in 51 (22 per cent) specimens, which included influenza a in 6 (3 per cent), influenza b in 3 (1 per cent), para-influenza type 1 in 3 (1 per cent), para-i ... | 1990 | 2313777 |
| association between respiratory syncytial virus outbreaks and lower respiratory tract deaths of infants and young children. | the temporal patterns of respiratory virus isolations from 10 laboratories in the usa were compared with that of deaths of children less than 5 years old from july 1975 through june 1984. isolations of respiratory syncytial virus (rsv) occurred as yearly winter outbreaks; parainfluenza virus 1 and 2 isolations occurred as well-defined outbreaks every other year in the autumn; parainfluenza virus 3 isolations occurred throughout the year with periodic, increased isolations suggestive of outbreaks ... | 1990 | 2319164 |
| severe respiratory syncytial virus pneumonia in an adult renal transplant recipient: successful treatment with ribavirin. | respiratory syncytial virus (rsv) pneumonia may be a severe disease especially in immunocompromised patients. it is quite rare in adults. we report a case of severe rsv pneumonia in a 38-year-old male renal transplant recipient with successful outcome after ribavirin aerosol therapy. | 1990 | 2320966 |
| isolation of respiratory syncytial virus from liver tissue and extrahepatic biliary atresia material. | respiratory syncytial virus (rsv) was isolated from liver tissue in a 7-month-old infant with extrahepatic biliary atresia. to our knowledge this is the first report of rsv isolation from human liver tissue. | 1990 | 2320967 |
| chest x-ray appearances in pneumonia and bronchiolitis. correlation to virological diagnosis and secretory bacterial findings. | chest x-ray findings were related to virus diagnosis, age and secretory bacterial findings in 128 infants and children under 7 years of age with clinical pneumonia and bronchiolitis. they belonged to a cohort examined in connection with the introduction of rapid methods for virological diagnosis. seventy-six children had a virus infection diagnosed by examination of nasopharyngeal secretion and/or by serological methods. thirty-seven of these children were classified as having pathogenic bacteri ... | 1990 | 2321485 |
| evaluation in chimpanzees of vaccinia virus recombinants that express the surface glycoproteins of human respiratory syncytial virus. | the immunogenicity and protective efficacy of recombinant vaccinia viruses that express the two major protective antigens of human respiratory syncytial virus (rsv), the f and g glycoproteins, were evaluated in chimpanzees. in previous studies in rodents and monkeys the f and g proteins expressed by the same recombinants were highly immunogenic and induced high levels of resistance to rsv replication following subsequent challenge. in contrast, in chimpanzees, a single intradermal immunization i ... | 1990 | 2336876 |
| screening for respiratory syncytial virus and assignment to a cohort at admission to reduce nosocomial transmission. | to limit nosocomial spread of respiratory syncytial virus (rsv) infection, a longitudinal intervention trial was instituted. nasal secretions or washes were screened for rsv antigen by enzyme-linked immunosorbent assay, and patients were assigned to an rsv-infected or an rsv-uninfected cohort. the baseline (preintervention) rate of 7.17 nosocomial cases of rsv per 1000 patient-days of care was used for comparison. despite continued infections in the community after screening was initiated, there ... | 1990 | 2348292 |
| an outbreak of an influenza-like illness in a nursing home. | as part of an ongoing infection surveillance program, data were collected on an outbreak of respiratory illness affecting 29% of residents in a 231-bed long-term care facility. viral titers could be collected on 60% of the ill residents and respiratory syncytial virus (rsv) was established in 14 of the symptomatic patients and was thought to be the probable cause of the outbreak. compared with previously reported long-term institutional outbreaks of rsv, there were significantly fewer cases of p ... | 1990 | 2358628 |
| productive infection of isolated human alveolar macrophages by respiratory syncytial virus. | respiratory syncytial virus (rsv) is a significant cause of lower respiratory tract disease in children and individuals with cell-mediated immunodeficiencies. airway epithelial cells may be infected with rsv, but it is unknown whether other cells within the lung permit viral replication. we studied whether human alveolar macrophages supported rsv replication in vitro. alveolar macrophages exposed to rsv demonstrated expression of rsv fusion gene, which increased in a time-dependent manner and co ... | 1990 | 2365811 |
| use of serum substitutes for the growth of four cell lines commonly used for virus isolation. | four serum extenders (opti-mem i, biorich, excyte i and excyte iii) and two serum substitutes (omni serum and serum plus) were evaluated for the reduction or replacement of fbs for the growth and maintenance of four representative cell lines used for virus isolation. mrc-5, human fetal foreskin fibroblast (hf), a549 and hep-2 cells were seeded into culture flasks containing mem with 10% fbs or with the serum extenders or substitutes and subcultured into 16 x 125 mm glass tubes and 1-dram shell v ... | 1990 | 2370288 |
| ambisense segment 4 of rice stripe virus: possible evolutionary relationship with phleboviruses and uukuviruses (bunyaviridae). | the complete nucleotide sequence of segment 4 of the rice stripe virus (rsv) genome was determined from overlapping cdna clones and by direct rna sequencing. the segment has two long open reading frames (orfs). one of the orfs (534 bases) is in the 5' region of the viral sequence, and the other (858 bases) is in the viral complementary sequence of the viral 3' region. such ambisense genome organizations have been seen previously in rnas of phleboviruses, uukuviruses and arenaviruses. the orf in ... | 1990 | 2374003 |
| detection of respiratory syncytial virus (rsv) infected cells by in situ hybridization in the lungs of cotton rats immunized with formalin-inactivated virus or purified rsv f and g glycoprotein subunit vaccine and challenged with rsv. | the replication of rsv in unimmunized cotton rats was evaluated by quantitating the amount of infectious virus in the lung and the number of rsv infected cells in a histopathological section of lung by in situ hybridization. rsv infected cells were detected only in alveoli and bronchioles and constituted only a small minority of the cell population. the temporal patterns of rise to the peak number of infected cells (day 4) and the peak titer of infectious virus (day 3) were similar. the clearanc ... | 1990 | 2385958 |
| igg-subclass-specific antibody reactivity to respiratory syncytial virus polypeptides investigated by western blot. | igg1 and igg3 subclass-specific antibody reactivity (abr) in serum samples obtained from infants and children in relation to acute lower respiratory disease caused by respiratory syncytial virus (rsv) infection was investigated by western blot. igg1 abr was directed against the nucleocapsid polypeptides vpn and vpp as well as against the glycoproteins gp48 (f1) and gp90. igg3 abr was directed only against vpn and vpp. in infants, a low igg1 reactivity against glycoproteins was observed. when ser ... | 1990 | 2392617 |
| gene transfer, expression and inheritance of prsv-rainbow trout-gh cdna in the common carp, cyprinus carpio (linnaeus). | a recombinant plasmid containing the rous sarcoma virus-long terminal repeat (rsv-ltr) promoter linked to rainbow trout (salmo gairdneri) growth hormone (gh) cdna was microinjected into fertilized carp eggs. genomic dna extracted from pectoral fin of individual presumptive transgenic fish was analyzed by dot blot and southern blot hybridization, using the rsv-ltr and/or the gh cdna sequences as probes. out of 365 presumptive transgenic fish analyzed, 20 individuals were found to contain prsv-rtg ... | 1990 | 2393581 |
| [a bronchiolitis epidemic caused by respiratory syncytial viruses]. | in the typical period of the year (autumn-winter 1986-1987) an outbreak of bronchiolitis in infants caused by respiratory syncytial virus (rsv) occurred. the acute course of the illness, a severe clinical picture, and a very fast spread of infection called for the very rapid techniques for detecting the etiological agent. applying the direct immunofluorescent antibody test (dfta) in samples of nasopharyngeal secretions, rsv was found to be responsible for the epidemic. later the same samples of ... | 1990 | 2396494 |
| impact of treatment guidelines on use of ribavirin. | during the 1987 through 1988 seasonal peak of respiratory syncytial virus (rsv), 177 courses of ribavirin were administered at st christopher's hospital for children, a tertiary care medical center in philadelphia, pa. charts were reviewed on 100 treated patients with proved or suspected rsv disease to determine adherence to american academy of pediatrics treatment guidelines. ninety-four percent fulfilled criteria for the risk of significant morbidity: cardiac, pulmonary, or immunodeficiency co ... | 1990 | 2396611 |
| interleukin-1-inhibitor activity induced by respiratory syncytial virus: abrogation of virus-specific and alternate human lymphocyte proliferative responses. | respiratory syncytial virus (rsv) infection has been shown to induce human mononuclear leukocyte (mnl) production of net interleukin-1 (il-1)-inhibitor activity. in the current studies of il-1-inhibitor effects, rsv-exposed cells were compared with autologous mnl that were sham-exposed or exposed to inactivated rsv or influenza virus (which induces net il-1 activity and commonly elicits effective homotypic immunity). exposure of mnl to influenza virus or inactivated rsv resulted in increased exp ... | 1991 | 1984478 |
| effectiveness of enteric immunization in the development of secretory immunoglobulin a response and the outcome of infection with respiratory syncytial virus. | cotton rats were immunized via intranasal, intradermal, or enteric routes with respiratory syncytial virus (rsv) or a live recombinant vaccinia virus expressing the rsv f glycoprotein (vaccinia f). the animals were tested for the appearance of rsv-specific antibody responses in the serum, bronchoalveolar lavage, and nasal wash after immunization and for virus replication 4 days after intranasal challenge with rsv. rsv antibody response in the serum and respiratory tract was demonstrated in all i ... | 1991 | 1987374 |
| comparison of the virulence of wild-type thymidine kinase (tk)-deficient and tk+ phenotypes of vaccinia virus recombinants after intranasal inoculation of mice. | a recombinant vaccinia virus vector was constructed which expressed the major surface glycoprotein g of human respiratory syncytial virus (rsv) and the thymidine kinase (tk) gene of vaccinia virus. the virulence of this tk+ recombinant virus was compared with that of a tk- recombinant and the wild-type (wt) virus after intranasal inoculation of mice. respiratory infection with wt virus resulted in a lethal infection with widespread dissemination of virus. in contrast, infection with the tk- reco ... | 1991 | 1990060 |
| nosocomial transmission of respiratory syncytial virus in immunocompromised adults. | respiratory syncytial virus (rsv) isolates obtained from nine infected immunocompromised adult patients hospitalized during two consecutive winters (january through april 1987 and 1988) were collected and analyzed against a panel of monoclonal antibodies by an enzyme immunoassay. the history of the patients' illness, onset of symptoms, and date of initial isolation of virus was correlated with the hospital ward and time of hospitalization. three patients died of respiratory failure related to rs ... | 1991 | 1993745 |
| ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments. | the complete nucleotide sequence of rice stripe virus (rsv) segment 3 shows that it has two open reading frames, one in the viral-complementary sequence, which codes for the nucleocapsid protein, and the other in the viral-sense sequence. the non-coding region between the ambisense genes in rsv segment 3 contains several u and a tracts, as do the ambisense s segments of phleboviruses and uukuviruses. as we have previously shown that rsv segment 4 has an ambisense nature, this is the first instan ... | 1991 | 1993885 |
| pattern of respiratory syncytial virus epidemics in finland: two-year cycles with alternating prevalence of groups a and b. | time-resolved fluoroimmunoassay with monoclonal antibodies distinguishing between respiratory syncytial virus (rsv) group a and b strains was used to analyze their prevalence in finland during 1981-1990 among 3285 patients with laboratory diagnosis of rsv, most of them hospitalized. the group typing of antigens in 608 rsv-positive nasopharyngeal aspirates showed a regular alternation of group prevalence, following the cyclic occurrence of the virus. group a predominated in 73%-90% of specimens f ... | 1991 | 1995719 |
| vaccination of cotton rats with a chimeric fg glycoprotein of human respiratory syncytial virus induces minimal pulmonary pathology on challenge. | the cotton rat model of human respiratory syncytial virus (rsv) infection was used to study the safety and efficacy of a chimeric fg glycoprotein that was expressed in insect cells using a baculovirus vector. histologic and virologic examination of vaccinated rat lungs was done after challenge with rsv. when rats were challenged 1 month after vaccination, severe pulmonary inflammation characterized by both a mononuclear and polymorphonuclear cell infiltrate and 30%-40% involvement of lung tissue ... | 1991 | 1995720 |
| respiratory syncytial virus (rsv) f, g, m2 (22k), and n proteins each induce resistance to rsv challenge, but resistance induced by m2 and n proteins is relatively short-lived. | the ability of recombinant vaccinia viruses that separately encoded 9 of the 10 known respiratory syncytial virus (rsv) proteins to induce resistance to rsv challenge was studied in balb/c mice. resistance was examined at two intervals following vaccination to examine early (day 9) as well as late (day 28) immunity. balb/c mice were inoculated simultaneously by the intranasal and intraperitoneal routes with a recombinant vaccinia virus encoding one of the following rsv proteins: f, g, n, p, sh, ... | 1991 | 1995956 |
| respiratory syncytial virus infection of human mononuclear phagocytes stimulates synthesis of platelet-activating factor. | production of platelet-activating factor 1-o-alkyl-2-acetyl-sn-glycero-3- phosphocholine (paf), a potent mediator of inflammation, by mononuclear phagocytes varies with their stage of cellular differentiation and the nature of the eliciting stimulus. the human monocytic cell line u937 can be induced to differentiate to a macrophage-like cell following phorbol myristate acetate exposure, and after differentiation, these cells efficiently support replication of respiratory syncytial virus (rsv). u ... | 1991 | 2005091 |
| [virologic diagnosis of interstitial pneumonia]. | viral pneumonia is not very frequent in healthy adults; it is mostly observed in elderly subjects infected with an influenza virus, a respiratory syncytial virus (rsv) or an adenovirus (adv). in immunocompromised subjects, cytomegalovirus (cmv) is the most common of causal agents. from the point of view of clinicians, virological diagnostic methods must be rapid and specific, and for this reason direct examination techniques for respiratory specimens and immunological detection of virus are curr ... | 1991 | 2008581 |
| dual-enzyme cascade-magnetic separation immunoassay for respiratory syncytial virus. | a new immunoassay developed for the detection of respiratory syncytial virus (rsv) makes use of magnetic separation and amplification by a dual-enzyme cascade for signal generation. magnetic particles are conjugated to monoclonal anti-rsv antibodies through the heterobifunctional crosslinker sulfosuccinimidyl 4-(maleimidomethyl)cyclohexane-1-carboxylate, yielding particles of high specific activity and low background. the dual-enzyme cascade is initiated by activation of a masked inhibitor for t ... | 1991 | 2010616 |
| multicenter study of strains of respiratory syncytial virus. | two major groups of respiratory syncytial virus (rsv) strains, a and b, have been identified and their patterns of isolation determined in different communities but not simultaneously in multiple communities. in this study, we tested 483 rsv isolates from 14 university laboratories in the united states and canada for the 1984/1985 and 1985/1986 rsv seasons; 303 (63%) isolates were group a, 114 (24%) were group b, and 66 (14%) could not be grouped. isolates were subdivided into six subgroups with ... | 1991 | 2010623 |
| immunity to and frequency of reinfection with respiratory syncytial virus. | to better understand the duration of immunity against respiratory syncytial virus (rsv) and the role of serum antibodies to the surface glycoproteins, f and g, in susceptibility to reinfection, 15 adults with previous natural rsv infection were challenged with rsv of the same strain group (a) at 2, 4, 8, 14, 20, and 26 months after natural infection. by 2 months about one-half and by 8 months two-thirds of the subjects became reinfected. each challenge resulted in infection in at least one-fourt ... | 1991 | 2010624 |
| antigenic and genomic diversity within group a respiratory syncytial virus. | antigenic analysis using monoclonal antibodies and genomic analysis using ribonuclease protection was done on 47 isolates of group a respiratory syncytial virus (rsv) recovered from children in st. louis during four rsv seasons. antigenic analysis identified four subgroups; of the three that included more than one member, those designated a/2 and a/2v had characteristic ribonuclease protection patterns. a third subgroup, a/4, exhibited more extensive genomic heterogeneity, but all isolates were ... | 1991 | 2010638 |
| respiratory syncytial virus infections on an adult medical ward. | eleven cases of respiratory syncytial virus (rsv) infection occurred in acutely ill hospitalized adults over a 7-week period. nosocomial illness was suspected in two patients. because rsv can cause serious infections in immunocompromised adults with the potential for nosocomial spread, the following recommendations are indicated: (1) during the winter months, early recognition and diagnosis of rsv infections both in hospital staff and in patients should be encouraged; (2) infected hospital perso ... | 1991 | 2012452 |
| complete nucleotide sequence of rna 3 of rice stripe virus: an ambisense coding strategy. | the rna 3 of rice stripe virus (rsv) isolate t was cloned and its nucleotide sequence was determined. the complete primary structure was found to consist of 2504 nucleotides. one putative open reading frame (orf), between nucleotides 2412 and 1444 in the 5'-proximal region of the virus complementary-sense rna, encoded a 322 amino acid protein with an mr of 35,134 that was identified as the coat protein. the other orf, between nucleotides 66 and 701 in the 5'-proximal region of the virus-sense rn ... | 1991 | 2016591 |
| management of the infant with respiratory syncytial virus. | this article examines updated clinical information concerning respiratory syncytial virus (rsv) infection including epidemiology, pathology, clinical manifestations, diagnosis, treatment, nosocomial infection, and prognosis. also presented is current information on ribavirin therapy, its side effects, and precautions. research related to the most effective isolation methodology is discussed, as well as nursing diagnoses based on gordon's functional health patterns and interventions for the infan ... | 1991 | 2019971 |
| human and bovine respiratory syncytial virus: immunopathologic mechanisms. | human respiratory syncytial virus (hrsv) is the major respiratory tract pathogen of infants and young children. bovine respiratory syncytial virus (brsv) is recognised as an important cause of respiratory tract disease in calves. both of these viruses and their respective diseases share many similarities. immunopathologic mechanisms have been proposed to be involved in the pathogenesis of respiratory syncytial virus (rsv) infections. this review examines the current understanding of the role of ... | 1991 | 2021054 |
| cytotoxic t cell activity against the 22-kda protein of human respiratory syncytial virus (rsv) is associated with a significant reduction in pulmonary rsv replication. | recombinant vaccinia viruses expressing the rsv f glycoprotein (vac-f), or a previously described chimeric protein consisting of the extracellular domains of the f and g glycoproteins (vac-fg), or the 22-kda membrane protein (vac-22 kda) were evaluated for their ability to protect balb/c mice against infection by rsv subgroup a or subgroup b viruses and for their ability to induce a humoral immune response or a cytolytic t lymphocyte (ctl) response. immunization with vac-f or vac-fg fully protec ... | 1991 | 2024493 |
| risk factors for respiratory syncytial virus-associated lower respiratory illnesses in the first year of life. | the relation of breast feeding and other factors to the incidence of respiratory syncytial virus-associated lower respiratory tract illness (rsv-lri) in the first year of life is examined. the study population is 1,179 healthy infants enrolled at birth between may 1980 and january 1984 into the tucson children's respiratory study, tucson, arizona. each subject's data were assessed at each month of age during the first year of life, during those months when respiratory syncytial virus was isolate ... | 1991 | 2035517 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by shell vial technique. | a shell vial technique was used to recover respiratory syncytial virus (rsv) from frozen nasopharyngeal specimens previously tested by rapid diagnostic methods. with specimens determined to be positive by direct fluorescence assay (dfa), the shell vial technique was at least as sensitive as conventional tissue culture (92 versus 90%). the majority of rsv isolates were detected within 16 h postinoculation, versus an average of 4.5 days by conventional techniques. also, the shell vial method recov ... | 1991 | 2037662 |
| comparison of enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and virus isolation for detection of respiratory viruses in nasopharyngeal secretions. | nasopharyngeal secretions obtained from 94 children with acute respiratory illness were examined for the presence of respiratory syncytial virus (rsv), adenovirus, and influenza virus type a by virus culturing (virus isolation technique [vit]), immunofluorescence assay (ifa), and enzyme-linked immunosorbent assay (elisa). similar results were obtained in at least two tests for rsv, influenza virus type a, and adenovirus in 92 (97.9%), 88 (93.6%), and 88 (93.6%) cases, respectively. both rapid vi ... | 1991 | 2037663 |
| comparison of two rapid methods for detection of respiratory syncytial virus (rsv) (testpack rsv and ortho rsv elisa) with direct immunofluorescence and virus isolation for the diagnosis of pediatric rsv infection. | the ability of two commercial immunoassays to detect respiratory syncytial virus (rsv) in respiratory specimens was evaluated as follows: 152 specimens were tested by testpack rsv (abbott), and 72 were tested by ortho rsv elisa (ortho). test outcomes were compared with those of virus isolation alone, direct immunofluorescence assay (dfa) alone, or virus isolation and/or dfa. testpack rsv versus virus isolation showed 91% sensitivity, 96% specificity, 93% positive predictive value (ppv), and 95% ... | 1991 | 2037684 |
| effect of passive antibody on the immune response of cotton rats to purified f and g glycoproteins of respiratory syncytial virus (rsv). | the effect of passively transferred rsv immune serum on the antibody response to a single dose of purified rsv fusion (f) and large (g) glycoproteins was studied in cotton rats. passively transferred antibody that achieved serum antibody levels similar to those seen in newborn human infants resulted in a seven- to eightfold suppression of the neutralizing antibody response of cotton rats to low doses of purified f and g glycoproteins (0.2-1.7 micrograms) and a twofold suppression to higher doses ... | 1991 | 2042390 |
| a comparison of transcriptional regulatory element activities in transformed and non-transformed rat anterior pituitary cells. | transformed (gh-3) and non-transformed (p3) rat anterior pituitary cells were compared in their ability to direct expression of plasmids containing a variety of eukaryotic transcriptional regulatory elements (tres). these include the herpes simplex virus thymidine kinase (hsv-tk), rous sarcoma virus long terminal repeat (rsv-ltr), simian virus 40 early (sv-40e), human cytomegalovirus immediate-early (cmv-ie) and mouse metallothionein 1 (mmt-1) tres. chloramphenicol acetyl transferase (cat) gene ... | 1991 | 2050277 |
| sequence analysis of the polymerase l gene of human respiratory syncytial virus and predicted phylogeny of nonsegmented negative-strand viruses. | the complete nucleotide sequence of the large (l) polymerase gene of human respiratory syncytial virus (rsv) strain a2 was determined by analysis of cloned-cdnas representing the entire gene and confirmed in part by dideoxy sequencing of genomic rna. the rsv l gene is 6578 nucleotides in length and contains a single major open reading frame that encodes a protein of 2165 amino acids. the molecular weight (250,226) and amino acid composition of the deduced rsv l protein are similar to those of ot ... | 1991 | 2053282 |
| rapid identification of respiratory syncytial virus infections by direct fluorescent antibody testing: reliability as a guide to patient cohorting. | this study compared the results of a commercially available, direct fluorescent antibody (dfa) test with viral culture in 880 specimens obtained from 690 patients by means of nasopharyngeal swabs. the two tests were congruent in 92.5% (814) of the specimens. the sensitivity of the dfa was 0.95, the specificity was 0.91, the positive predictive value was 0.82, and the negative predictive value was 0.98. among 548 inpatients, there were 3 mixed infections (rsv and another virus), 8 rsv infections ... | 1991 | 2053715 |
| group-specific serum antibody responses in children with primary and recurrent respiratory syncytial virus infections. | antigenic group-specific serum antibody responses to first and second respiratory syncytial virus (rsv) infections were studied in children who had been followed longitudinally from early infancy in a research day-care center. plaque-reduction neutralizing (prn) antibody assays and elisas for the fusion (f) and attachment (g) glycoproteins were done using antigens of prototype rsv strains from groups a and b. responses to antigens of viruses homologous and heterologous to the antigenic group of ... | 1991 | 2056202 |
| evaluation of an enzyme membrane immunoassay (directigen rsv) for the rapid diagnosis of respiratory syncytial virus infections. | a rapid enzyme immunoassay (eia) membrane test, the directigen respiratory syncytial virus (becton dickinson), was compared with cell culture, an indirect immunofluorescence (if) test, the monofluokit respiratory syncytial virus (diagnostics pasteur), and a conventional enzyme immunoassay antigen test, the abbott respiratory syncytial virus enzyme immunoassay in nasal aspirates specimens from children with suspected respiratory syncytial virus (rsv) bronchiolitis. the sensibility and specificity ... | 1991 | 2062553 |
| early use of indirect immunofluorescence for the detection of respiratory syncytial virus in hep-2 cell culture. | respiratory syncytial virus is detected in cell culture by the presence of cytopathic effect. to detect rsv before cytopathic effect is usually seen, slides were evaluated retrospectively from 482 hep-2 cell cultures on days 2-4 after inoculation. indirect immunofluorescent staining detected rsv in 57 of 94 cultures that eventually were found positive by cytopathic effect. in an additional 19 cases that ultimately showed no cytopathic effect, rsv also was detected. in 15 of the latter cases, the ... | 1991 | 2069130 |
| respiratory syncytial virus infection in c57bl/6 mice: clearance of virus from the lungs with virus-specific cytotoxic t cells. | we describe respiratory syncytial virus (rsv)-specific cytotoxic t-cell (ctl) lines and clones developed from the spleens of c57bl/6 and balb/c mice. line 7 and clones derived from it were h-2kb restricted, whereas line 12 had both kb and db components. both lines, and all the clones except one, could lyse targets infected with either strain a or strain b rsv. line 7 or 7-11e1 cells (8 x 10(6) to 10 x 10(6) given intravenously cleared rsv from the lungs of infected mice. there was no morbidity o ... | 1991 | 2072460 |
| cytokine (tumor necrosis factor, il-6, and il-8) production by respiratory syncytial virus-infected human alveolar macrophages. | human alveolar macrophages (am) are susceptible to infection with respiratory syncytial virus (rsv), but the infection is abortive after the initial cycles of virus replication. we have investigated if rsv infection of am results in the production of cytokines tnf, il-6, and il-8, all of which may modulate inflammatory and immune responses to the virus, as well as may directly protect respiratory epithelial cells against spread of infection. within 1 h after interaction with rsv, increased mrna ... | 1991 | 1753101 |
| [detection of rs viral sequences in otitis media effusion from children]. | the role of respiratory syncytial virus (rs virus) in otitis media with effusion was studied. reverse transcriptase-polymerase chain reaction (rt-pcr) and nested polymerase chain reaction (nested pcr) were used to provide accurate methods for detection of rs viral sequences in the otitis media effusion from children. we could find rs viral sequences in 10/12 samples tested which had been collected during and even after natural outbreak of rsv in the community. these observations suggest rs virus ... | 1991 | 1757848 |
| occurrence of respiratory syncytial virus subtypes in hospitalized children in cleveland, ohio from 1985 to 1988. | in order to determine the frequency of occurrence of the two respiratory syncytial virus (rsv) subtypes in hospitalized children in cleveland, ohio, we analyzed clinical isolates obtained during three consecutive winter epidemic seasons between 1985 and 1988. rsv was recovered from the frozen clinical specimens of 197 patients: 176 subtype a, and 21 subtype b. subtype a predominated during all three epidemic seasons, ranging from 83 to 94% of isolates. we surveyed the clinical records of 16 chil ... | 1991 | 1758737 |
| the cold war against rsv. | respiratory syncytial virus (rsv) can cause substantial morbidity and mortality in high risk patients. the authors discuss prevention and treatment of this virus. | 1991 | 1765449 |
| immune responses of lambs experimentally infected with bovine respiratory syncytial virus and pasteurella haemolytica. | the immune responses of lambs experimentally infected with bovine respiratory syncytial virus (rsv) and pasteurella haemolytica were compared with lambs infected with bovine rsv or pasteurella haemolytica alone. superinfection with p. haemolytica aggravated the reduction in the number of cd5+, cd4+ and lca p220+ (b) lymphocytes caused by bovine rsv, but it had no effect on the neutralizing antibodies against p. haemolytica cytotoxin. serum samples obtained from lambs experimentally infected with ... | 1991 | 1779037 |
| evaluation of a commercial enzyme immunomembrane filter assay for detection of respiratory syncytial virus in clinical specimens. | a commercial enzyme immunomembrane filter assay (eifa) for respiratory syncytial virus (rsv) was compared prospectively with isolation in cell culture and an enzyme immunoassay. a total of 595 respiratory specimens, mostly from pediatric patients, were examined. the eifa was 70.96% sensitive and 72.40% specific in comparison with cell culture. results for 40 specimens (6.72%) were uninterpretable, mainly due to filtration difficulties. twenty-one (25%) of 84 specimens whose results were initiall ... | 1991 | 1802701 |
| failure to detect paramyxovirus sequences in paget's disease of bone using the polymerase chain reaction. | it is widely believed that paget's disease of bone is due to a "slow virus" infection of osteoclasts with one of the paramyxovirus group. controversy continues to surround the identity of the virus involved, however, since at different times evidence has been presented implicating measles virus (mv), respiratory syncytial virus (rsv), and canine distemper virus (cdv) as putative infective agents. in this study we used the technique of reverse transcription and polymerase chain reaction (pcr) to ... | 1991 | 1805546 |
| comparative study of respiratory syncytial virus in nasopharyngeal aspirates using conventional cell culture, shell viral centrifugation culture, immunofluorescence and biotin-avidin enzyme linked immunosorbent assays. | 133 nasopharyngeal aspirates (npa) were simultaneously tested for the presence of respiratory syncytial virus (rsv) by conventional cell culture (ccc), shell vial centrifugation culture (svc), immunofluorescence assay (ifa) and biotin-avidin enzyme linked immunosorbent assay (b-a elisa). these yielded positive results in 32(24%), 45(33.8%), 36(27%) and 40(30%) of specimens, respectively. specimens positive by ifa and b-a elisa were all also positive by svc. the sensitivity of ccc, ifa, and b-a e ... | 1991 | 1807259 |
| expression of a human monoclonal anti-hiv-1 antibody in cho cells. | the cdna coding for the light and heavy chains, respectively, of the human monoclonal antibody 3d6 (igg1, kappa), which binds specifically to human immunodeficiency virus-1 (hiv-1) gp41, was inserted into three different mammalian expression vectors and transfected into chinese hamster ovary (cho) cells. transcription was under the control of rous sarcoma virus long terminal repeat (rsv ltr), human cytomegalovirus major immediate early (cmv ie) promoter, and mouse mammary tumor virus long termin ... | 1991 | 1809191 |