Publications

isolation and genetic characterization of temperature-sensitive mutants that define five additional recombination groups in simian rotavirus sa11.	nineteen independent temperature-sensitive (ts) mutants were isolated from sa11 following mutagenesis with proflavin or 5-azacytidine. fourteen of the ts mutants fell into one or another of five mutant groups previously defined by recombination. five of the ts mutants defined five recombination groups (f, g, h, i, and j) that had not been previously identified. thus, 10 of the 11 expected mutant groups have been identified in sa11. the prototype mutants of the 10 mutant groups were tested for re ...	1983	6316640
isolation of new serotypes of bovine rotavirus. brief report.	two cytopathogenic bovine rotavirus strains were isolated. the infectivity of both isolates was relatively stable at ph 3.0 and resistant to ether. their replication was not affected by 5-iodo-2'-deoxyuridine. the new isolates shared immunofluorescent antigen with the lincoln strain of bovine rotavirus. by neutralization test, however, these strains were clearly distinguished from one another.	1983	6316874
antibody response in rabbits following oral administration of human rota-, calici- and adenovirus. brief report.	rota-, calici-, adeno- and astrovirus extracted from human faeces were administered orally to eight rabbits. antibody rise was demonstrated in some of the animals against rota-, calici- and adenovirus. clinical illness or virus excretion was not observed.	1983	6316877
virological survey of children in nyeri and mombasa. monthly survey of rotavirus in faeces.		1983	6317333
ultrastructural evidence for the cellular uptake of rotavirus by endocytosis.	the ultrastructural aspects of rotavirus sa11 infection were studied in ma104 cells, with particular attention directed to the early stages of virus adsorption and penetration. within the first few minutes postadsorption, rotavirus was seen at the plasma membrane, associated with coated pits and coated vesicles, indicating that sa11 virus is taken into the cell by adsorptive or receptor-mediated endocytosis. multiple virus particles were seen, beginning at 1 h and continuing throughout infection ...	1983	6317607
protection studies in colostrum-deprived piglets of a bovine rotavirus vaccine candidate using human rotavirus strains for challenge.	studies were performed to evaluate the potential use of the bovine rit 4237 rotavirus strain as a vaccine candidate against infection with human rotaviruses. initial experiments revealed that colostrum-deprived piglets were susceptible to infection with several human strains, except for those belonging to subgroup 1. subsequently, different immunization procedures with rit 4237 were studied in this animal model. it was found that a two-dose administration, either given intramuscularly (twice) or ...	1983	6317767
an atypical rotavirus detected in a child with gastroenteritis in rio de janeiro, brazil.		1983	6318014
development of vaccines against rotavirus disease.		1983	6318264
lifestyle and antiviral antibody studies among homosexual men in denmark.	two-hundred and fifty-nine male homosexuals (hs) of a large and a small danish town were studied for antibodies to various virus and their statistical relationship to sex habits, drug use and other lifestyle factors. prevalence rates against cytomegalovirus (cmv) were 86.7% of hs men from copenhagen and 73.3% of hs men from the smaller community of aarhus, against 30% of a control group. antibody prevalence in homosexuals was significantly related to number of years of homosexual activity (p les ...	1983	6318513
[clinical symptoms and therapeutic possibilities in rotavirus infections].	author reviews his personal experience on 189 cases of gastroenterocolitis produced by rotavirus during a two year period (1980-81). after exposing diagnostic and clinical features, he brings into consideration future possible therapy of this viral infection.	1983	6318619
[attempt at the experimental reproduction of rotavirus diarrhea in newborn calves deprived of colostrum].	colostrum-deprived newborn calves were orally inoculated with different doses of cell-culture bovine rotavirus or with bacterium-free filtrates of calf stools containing rotavirus. none of the animals that received high doses of cell-culture rotavirus developed diarrhoea or any other clinical sign, although all of them excreted virus for several days and produced specific antibodies; calves inoculated with lower doses of cell-culture virus or with stool filtrates showed a transient diarrhoea 48 ...	1983	6318646
concentration of rotavirus and enteroviruses from blue crabs (callinectes sapidus).	a simple method for concentration and detection of rotavirus and enteroviruses in the blue crab is described. virus was separated from tissue homogenates at ph 9.5, concentrated by adsorption to protein precipitates at ph 3.5, and recovered by elution of precipitates at ph 9.2. test samples of 12 to 15 ml were produced from an initial 100 g of crab tissues. cat-floc precipitation was used to remove sample toxicity for cell cultures. recovery effectiveness averaged 52% with poliovirus 1, echoviru ...	1983	6318667
[demonstration of an experimentally induced antigenic drift in rotaviruses].		1983	6318682
electron microscopic identification of rotavirus group antigen with gold-labelled monoclonal igg.	colloidal gold was complexed with monoclonal igg to the inner capsid of human rotavirus. the complex was then used as an immunoelectron microscopic marker to identify the location of the group-specific antigen of rotavirus.	1983	6318697
comparison of enzyme-linked immunosorbent assay, electron microscopy, and reversed passive haemagglutination for detection of human rotavirus in stool specimens.	an enzyme-linked immunosorbent assay (elisa) using microplates as solid phase, rabbit antiserum against human rotavirus wa strain as catching antibody, and the same reagent labeled with beta-d-galactosidase as conjugate, has been developed for detection of human rotavirus antigen(s) in stool specimens from patients with acute gastroenteritis. the limit of detection of purified human rotavirus by elisa was 15.6 ng/ml (1.56 ng/well) of viral protein. the sensitivities of elisa, electron microscopy ...	1983	6318724
rotavirus inactivation by chemical disinfectants and antiseptics used in hospitals.	nosocomial outbreaks of rotaviral gastroenteritis are a common occurrence. although proper disinfection practices in the hospital environment are considered to be important in the prevention and control of such outbreaks, very little information has been available on the rotavirus-inactivating capacity of chemical disinfectants and antiseptics commonly used in hospitals. in view of this, 11 such products were selected and screened for their capacity to bring about at least a 3 log10 reduction in ...	1983	6318942
detection of rotavirus in horses with and without diarrhea by electron microscopy and rotazyme test.	a total of 142 equine fecal samples (93 field fecal and 49 experimental fecal specimens) were examined for rotavirus using direct electron microscopy (em) and the rotazyme test. eighty-six stool specimens were diarrhea samples. the rotazyme test sensitivity and accuracy as compared to em was determined by the visual (color reaction) and spectrophotometric methods. the overall agreement was 94.8% and 92.3% between em and rotazyme visual and spectrophotometric methods, respectively when suspect re ...	1983	6309476
isolation, propagation, and characterization of a second equine rotavirus serotype.	a rotavirus designated strain h-2 was isolated in primary african green monkey kidney cells from a foal with diarrhea. this cell culture-adapted strain was found to be similar, if not identical, to simian rotavirus (strains mmu18006 and sa-11) and canine rotavirus (strain cu-1) and, in addition, demonstrated a one-way antigenic relationship with five human rotavirus strains (p, b, no. 14, no. 15, and yo) of the third human rotavirus serotype by the plaque reduction neutralization test. this is t ...	1983	6309657
passive immunity to bovine rotavirus in newborn calves fed colostrum supplements from immunized or nonimmunized cows.	colostrum was collected and pooled from each of five cows in three experimental groups: group i cows received intramuscular and intramammary inoculations of adjuvanted modified live ohio agricultural research and development center rotavirus vaccine; group ii cows were injected intramuscularly with a commercial modified-live rota-coronavirus vaccine; and group iii cows were uninoculated controls. pooled colostrum from group i cows had higher (p less than 0.05) enzyme-linked immunosorbent assay ( ...	1983	6309660
pediatric viral gastroenteritis during eight years of study.	during the period january 1974 through july 1982, fecal samples from 1,537 pediatric inpatients with gastroenteritis were tested for enteric viruses by electron microscopic and rotavirus enzyme-linked immunosorbent assay techniques. rotaviruses were detected in 34.5% of these patients, enteric adenoviruses were detected in 4.7%, approximately 27-nm viruses were detected in 1.6%, and at least one of these agents was found in 40.1% of the study subjects. three infections were by an apparently new ...	1983	6309901
infectious gastroenteritis in norfolk island and recovery of viruses from drinking water.	a high incidence of gastroenteritis in both islanders and tourists has been recorded in recent years on norfolk island--a popular tourist resort for australians and new zealanders. no bacterial cause has been found. however, electron microscopic examination of 28 faecal specimens revealed viruses associated with gastroenteritis in 21 (75%). no viruses were isolated in cell cultures. bore water is used for drinking purposes on the island and 32 samples from 15 bores were examined for viruses by e ...	1983	6309956
association of rotavirus infection with kawasaki syndrome.		1983	6309994
use of electrophoresis of rna from human rotavirus to establish the identity of strains involved in outbreaks in a tertiary care nursery.	during two nosocomial outbreaks in a tertiary care nursery, 22 of 102 infants had infections due to human rotavirus (hrv). the time sequence and proximity of the infected infants in the nursery rooms had suggested infant-to-infant spread of a single hrv strain in each outbreak. polyacrylamide gel electrophoresis of virus permitted demonstration of four different hrv electropherotypes (strains) during the first outbreak period and seven during the second. one of the strains was detected during bo ...	1983	6309996
characteristics and analysis of electropherotypes of human rotavirus isolated in chile.	electropherotypes of human rotavirus from stool samples of hospitalized and ambulatory infants with acute diarrhea were analysed. thirty-two different electropherotypes were observed in 142 cases in which the viral genome rna was characterized. the seasonal distribution of the electropherotypes, from 1979 to 1981, showed that the same electropherotypes were detected throughout the period of study while others were found only occasionally. electropherotypes were also analyzed depending on whether ...	1983	6309997
human rotaviruses and genome rna.		1983	6309998
the tecumseh study. xii. enteric agents in the community, 1976-1981.	enteric illnesses in residents of tecumseh, michigan, were studied from 1976 to 1981. the frequency of illness among adults and children increased each year in the late autumn, usually in november. this peak of illness preceded the main period of appearance of the rotaviruses, which were less regularly associated with another peak of illness. rotaviruses were identified in 3.8% of all stool specimens collected; in specimens from children under two years of age, the annual rate of rotavirus ident ...	1983	6310002
the molecular biology of rotaviruses. vi. rna species-specific terminal conservation in rotaviruses.	the use of t1 rnase fingerprinting of terminally labelled genomic double-stranded rna species from various rotavirus isolates, to analyse the near terminal g-residue positions, has revealed an rna species-specific fingerprint pattern covering approximately 40 nucleotides at the termini. these rna species-specific terminal fingerprint patterns were found to be conserved in both rotavirus rnas isolated from various animal species, and in isolates from a single animal species where gross divergence ...	1983	6310030
an outbreak of rotavirus-associated neonatal necrotizing enterocolitis.	an outbreak of necrotizing enterocolitis and hemorrhagic gastroenteritis occurred in two nurseries during 25 days in august 1982. eleven of the 40 patients in these nurseries during that time developed disease (attack rate 27.5%). in seven of the 10 patients with gastrointestinal disease, stool samples tested for human rotavirus were positive by elisa, whereas in 20 unaffected infants, no stools tested demonstrated hrv (p = 0.0001). eleven staff members had serologic evidence of recent hrv infec ...	1983	6310072
completion of the gene coding assignments of sa11 rotavirus: gene products of segments 7, 8, and 9.	improved fractionation of double-stranded rna segments 7, 8, and 9 of simian rotavirus sa11 has permitted their isolation and individual translation in vitro. segment 7 codes for p31 (ns2), segment 8 codes for p33 (ns1), and the segment 9 gene product resembles the gp34 precursor observed in sa11 virus-infected cells. in vitro glycosylation of translation products of segments 5 and 10 was also observed.	1983	6310157
[incidence of viral particles in children with acute or protracted diarrhea, seen at the hospital das clinicas of the faculdade de medicina de botucatu from february 1980 to february 1981].		1983	6310721
[rotavirus gastroenteritis in pediatrics].	during a one year period, 2.179 stool specimen coming from 1.608 children attending our hospital, were reviewed. these children were both with or without gastrointestinal symptomatology. rotavirus determination by an elisa method and routine stool culture were performed to every specimen. rotavirus incident in the symptomatic group is 27,61%, meanwhile bacterial pathogens represent a 27,29%. it is studied the seasonal variation of virus emission and its distribution depending on children age. ac ...	1983	6311067
response of mice to rotaviruses of bovine or primate origin assessed by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization.	sera from (i) gnotobiotic balb/c, cd-1, and cfw mice and (ii) conventional balb/c mice were evaluated by radioimmunoassay, radioimmunoprecipitation, and plaque reduction neutralization, using the wa, sa-11, and wc-3 (bovine) strains of rotavirus as the detecting antigens. the gnotobiotic mice had no antirotavirus antibody detectable by radioimmunoprecipitation and no neutralizing antibody at a dilution of 1:50 by plaque reduction neutralization. all sera from the conventional mice had rotavirus- ...	1983	6311747
the cultivation of human rotavirus, strain 'wa', to high titer in cell culture and characterization of the viral structural polypeptides.	the structural proteins of the 'wa' (serotype 2) strain of human rotavirus have not been described previously. single-cycle virus growth in ma-104 cells using 5 micrograms/ml of trypsin in the growth medium was rapid with maximal viral yields (approximately 10(6) pfu/ml) obtained 10-12 h post-infection. there was a continuous progression of cytopathic effect (cpe) from 6- to 5-h post-infection. under conditions of multiple-cycle growth, a greater concentration of trypsin (40 micrograms/ml) in th ...	1983	6311856
direct isolation in cell culture of human rotaviruses and their characterization into four serotypes.	of 73 rotavirus-positive fecal specimens tested, 39 yielded a human rotavirus that could be cultivated serially in ma104 or primary african green monkey kidney cells or both; 18 were serotyped. four distinct serotypes were identified by plaque reduction or tube neutralization assay or both, and three of these serotypes were the same as those established previously by plaque reduction, using human rotaviruses cultivated by genetic reassortment with a cultivable bovine rotavirus. ten human rotavir ...	1983	6311872
antigenic relationships among some bovine rotaviruses: serum neutralization and cross-protection in gnotobiotic calves.	a method was further developed to screen non-tissue-culture-adapted bovine rotaviruses for serotype, using a neutralization test with infectious fecal rotavirus. one of those rotaviruses (b223) which was not blocked by antiserum to the neonatal calf diarrhea virus (ncdv) serotype was then adapted to cell culture in the presence of the antiserum for two or more passages and hyperimmune antiserum to this isolate had a 60-fold-higher homologous neutralization titer than with the ncdv serotype rotav ...	1983	6311873
molecular characterization of rotaviruses with distinct group antigens.	comparative serological and nucleic acid studies were carried out on rotavirus isolates, with and without the original group antigen. indirect immunofluorescence allowed the atypical isolates to be divided into two groups each with its own distinct group antigen hence giving a total of at least three distinct rotavirus groups. genome profile analysis of the atypical isolates again divided them into two groups which corresponded with those obtained by immunofluorescence and within which the overa ...	1983	6311947
characterization of rotaviral rna isolated from children with gastroenteritis in two hospitals in rotterdam.	rotavirus rna was extracted from stools collected from children with gastroenteritis in two hospitals in rotterdam. electrophoresis of the rnas showed that at least 19 different electropherotypes were isolated from stools collected in the period from 1977 to 1982. it was possible to identify the index case in some hospital-acquired cases of rotaviral gastroenteritis.	1983	6311968
detection of a human rotavirus strain different from types 1 and 2--a new subgroup? epidemiology of subgroups in a swedish and an ethiopian community.	a possible new subgroup of human rotavirus was found by crossed immunoelectrophoresis and enzyme-linked immunosorbent assay. the epidemiology of the established subgroups 1 and 2 and this new variant was studied in two different communities. of 398 rotavirus isolates from malmö, sweden, 26.8% were of type 1, 71.7% of type 2, and 1.5% of the new variant. corresponding figures for 384 samples from addis ababa, ethiopia, were 33.1% type 1 and 66.9% type 2. a total of 87% of the swedish and 79% of t ...	1983	6311970
detection of rotavirus in respiratory secretions of children with pneumonia.		1983	6312006
production and preliminary characterization of monoclonal antibodies directed at two surface proteins of rhesus rotavirus.	a series of monoclonal antibodies was isolated which reacted with one of two major surface proteins of rhesus rotavirus. thirty-six monoclonal antibodies immunoprecipitated the 82-kilodalton outer capsid protein, the product of the fourth gene, the viral hemagglutinin. these monoclonal antibodies exhibited hemagglutination inhibition activity and neutralized rhesus rotavirus to moderate or high titer. three monoclonal antibodies immunoprecipitated the 38-kilodalton outer capsid glycoprotein, the ...	1983	6312065
coding assignment and nucleotide sequence of simian rotavirus sa11 gene segment 10: location of glycosylation sites suggests that the signal peptide is not cleaved.	a cloned dna copy of simian rotavirus sa11 genomic segment 10 was used to confirm the assignment of the nonstructural glycoprotein ncvp5 to this gene. determination of the nucleotide sequence for gene 10 indicated that ncvp5 is 175 amino acids in length and has an n-terminal hydrophobic region with the characteristics of a signal sequence for membrane translocation. unexpectedly, this region was also the location for the only two potential glycosylation sites within the molecule, asparagine resi ...	1983	6312090
viral gastroenteritis.		1983	6312212
clinical uses of the diagnostic virology laboratory.		1983	6312218
clinical, virological and serological evaluation of the efficacy of peroral live rotavirus vaccination in calves kept under normal husbandry conditions.		1983	6306961
[use of the complement fixation test for the detection of rotavirus antibodies in cattle].		1983	6307201
[studies of the occurrence of diarrhea pathogens in experimental calves of the institute as a contribution to their epizootiology].		1983	6307202
[serological studies, by the complement fixation test, on the incidence of rotavirus infections in cattle in the cottbus district].		1983	6307203
[experiences obtained thus far in the immunoprophylaxis of rotavirus infections in calves].		1983	6307204
[use of different rotavirus vaccines (adsorbate and live virus vaccine) in dairy cattle units of the cottbus district].		1983	6307205
the effect of relative humidity and temperature on the survival of bovine rotavirus in aerosol.	the effect of relative humidity and temperature on the survival of bovine rotavirus in airborne particles was investigated. bovine rotavirus was found to be stable at low and high relative humidity; infectivity was lost more rapidly at high temperature than at lower temperature.	1983	6307226
value of stool examination in patients with diarrhoea.	findings of stool examinations in 1593 patients with diarrhoea due to a single enteric pathogen--enterotoxigenic escherichia coli rotavirus, shigella, campylobacter jejuni, vibrio cholerae 0:1, entamoeba histolytica, or giardia lamblia--were reviewed to determine how well they predicted the agent associated with the diarrhoea. specimens were examined visually for blood and mucus, tested for ph, and examined under a microscope for the presence of red and white blood cells, parasites, and stool fa ...	1983	6307460
prevalence of neutralizing antibodies against a candidate rotavirus vaccine in adults.	because of the morbidity and mortality from infection with rotaviruses in children under 2 years, in many parts of the world but especially in developing countries, an urgent need for a vaccine has been recognized. at present, a vaccine candidate of bovine origin, the rit 4237 strain vaccine developed from a strain received from mebus et al., is available. this strain has been shown to induce cross protection by challenge of colostrum deprived piglets with human rotavirus strains. before testing ...	1983	6307775
studies in volunteers with human rotaviruses.		1983	6307776
effect of feeding human milk on nosocomial rotavirus infections in an infants ward.	children aged 1-15 months hospitalized for different respiratory diseases (including otitis media) at an infants ward frequently developed diarrhoea during their stay in the clinic. bacteriological examinations of stool samples were usually negative but rotavirus excretion was frequent according to elisa tests. children fed with human milk are assumed to be less susceptible to rotavirus infections due to the protection by maternal antibody. in view of preventive measures for avoiding rotavirus n ...	1983	6307777
epidemiologic background for the need of rotavirus vaccine in finland. preliminary experience of rit 4237 strain of live attenuated rotavirus vaccine in adults.	epidemiologic evidence from finland indicates a dramatic change in the seasonal pattern of acute diarrhoea over the past 30 years. while in the 1950's the majority of cases occurred in late summer the seasonal peak in the 1970's was in winter and spring, coinciding with the prevalence of rotavirus infections. it is not possible to determine retrospectively the aetiologic agents involved in the summer diarrhoea, but epec may have been one of them, as epec are quite rare in finland today compared ...	1983	6307778
vaccination of cows with a combined rotavirus/enterotoxigenic "e. coli" k99 vaccine to protect newborn calves against diarrhoea.	the protective effect of a combined rotavirus/enterotoxigenic e. coli k99 vaccine against naturally occurring neonatal diarrhoea was evaluated in two dairy and two beef herds. before vaccination, frequency of diarrhoea in these herds was 46% up to 60% with losses of 7% to 20%. after vaccination of pregnant cows and heifers mild diarrhoea occurred in 14% to 20% of their calves, one out of 144 calves died with enteric symptoms. in 13 out of 26 calves of nonvaccinated controls where diarrhoea was n ...	1983	6307779
passive protection of newborn calves against rotavirus by vaccination of their dams.	the rotavirus is considered as being one of the major causes of acute gastroenteritis; it occurs in cattle farms where, either by itself or in association with other pathogenic agents, it is responsible for a high percentage of neonatal morbidity and mortality. over two consecutive years, field trials were done using inactivated rotavirus vaccine in oily excipient inoculated to pregnant cows. results obtained prove a significant increase in the rate of colostral antibodies and a very real prolon ...	1983	6307780
infection of baboons ("papio cynocephalus") with rotavirus (sa11).	a recent survey of nonhuman primate sera indicated that antibody to a rotavirus (sa11) was prevalent among a wide spectrum of animals. both new and old world species were found with antibody, many with surprisingly high titers (1:320). whether or not infection per se was due to sa11 or an antigenically closely related agent could not be determined by that study; however, it is apparent from this study as well as from a survey of the literature that natural as well as experimental infection of no ...	1983	6307781
characteristics of the cell-mediated immune response to rotavirus in suckling mice.	the kinetics of rotavirus specific cell mediated immunity and rotavirus infection were compared in infected and uninfected inbred balb-c mice. lymphocytes transformation (ltf) in spleen cells exposed to varying doses of mouse rotavirus (mrv) and control antigens was carried out at intervals up to 60 days following infection. rotavirus infection was monitored by the detection of rotavirus antigen in isolated small intestinal enterocytes using direct immunofluorescent assay. the severity of diarrh ...	1983	6307782
two new serotypes of enteric adenovirus causing infantile diarrhoea.	fastidious enteric adenovirus have recently been recognized as an important cause of acute gastroenteritis in young children. their inability to grow in vitro has hampered classification by conventional methods. with modern immunological and chemical techniques the enteric adenoviruses have been shown to be distinct from the 39 established human adenovirus serotypes. in a prospective study of the viral, bacterial and parasitic aetiology of acute gastroenteritis 410 children and 205 age-matched c ...	1983	6307784
detection of antigenically distinct rotaviruses from infants.	antigenically distinct rotaviruses, i.e., viruses morphologically identical to conventional rotaviruses by electron microscopy, yet lacking the common group antigen(s) detected by an enzyme-linked immunosorbent assay, were found in 2 of 51 fecal samples from bulgarian infants with rotavirus gastroenteritis. these antigenically distinct viruses contained 11 segments of double-stranded rna, but they demonstrated a unique rna migration profile after electrophoresis of the genome rna in polyacrylami ...	1983	6307873
rotavirus-associated traveler's diarrhea: neutralizing antibody in asymptomatic infections.	the sera of patients with traveler's diarrhea were assayed for rotavirus-specific immunoglobulins in enzyme-linked immunosorbent and micro-neutralization assays. the antigenically related simian rotavirus sa-11 was used in both assays. although similar titers of rotavirus-specific binding immunoglobulin g and a were observed in the sera of both symptomatic and asymptomatic subjects, clinical illness appeared to correlate with the failure to detect increased (greater than or equal to fourfold) ti ...	1983	6307883
human rotavirus detection by agglutination of antibody-coated erythrocytes.	we sensitized sheep erythrocytes (srbc) with antibodies against human rotavirus strain wa (srbc-antiwa) and antibodies against calf rotavirus strain ncdv (srbc-antincdv). these were readily agglutinated in the presence of homologous antigens, i.e., human rotavirus and calf rotavirus. by the hemagglutination of srbc-antiwa and srbc-antincdv (reverse passive hemagglutination [rpha]), titration of rotavirus in extracts from feces of children suffering from diarrhea (61 specimens) was carried out. w ...	1983	6308039
unreliability of rotazyme elisa test in neonates.		1983	6308197
nucleotide sequence of the gene encoding the serotype-specific glycoprotein of uk bovine rotavirus.	the nucleotide sequence of the gene which encodes the major outer-shell glycoprotein of uk bovine rotavirus has been determined. the dsrna genome segment encoding this protein was converted into ds cdna and cloned into pbr322 for sequence studies. the gene is 1062 base pairs in length and contains a single, long, open reading-frame capable of coding for a protein of 326 amino-acids. this would leave 5' and 3' non-coding regions of 48 and 36 nucleotides in the mrna. the predicted amino-acid seque ...	1983	6308556
[the viral incidence in childhood diarrhea].		1983	6309062
viral infections of infancy.		1983	6303679
experimental infection of adult tupaia belangeri yunalis with human rotavirus.		1983	6303715
serotypes of bovine rotaviruses distinguished by serum neutralization.	fourteen cytopathic bovine rotaviruses isolated from naturally affected calves were characterized serologically by serum neutralization. antisera were prepared in guinea pigs against the three isolates and the lincoln strain of bovine rotavirus. these four rotaviruses were segregated into at least two distinct serotypes (designated types 1 and 2) by serum neutralization. the remaining 11 isolates were also placed into two groups according to neutralization specificity by using the antisera again ...	1983	6303958
rotavirus inactivated by a hypochlorite-based disinfectant: a reappraisal.		1983	6304478
clinical immunity after neonatal rotavirus infection. a prospective longitudinal study in young children.	to determine whether rotavirus infection in newborn babies conferred immunity to postneonatal rotavirus infection, we studied 81 babies at birth and kept them under clinical and serologic study for three years. during the first 14 days of life, 44 of the infants excreted rotavirus, and 37 did not. fifty-five per cent of those with neonatal infection and 54 per cent of those without it had rotavirus infection during the next three years. symptoms associated with postneonatal rotavirus infection w ...	1983	6304516
cloning and sequence of uk bovine rotavirus gene segment 7: marked sequence homology with simian rotavirus gene segment 8.	the genome of the uk bovine rotavirus, which consists of eleven segments of dsrna was polyadenylated and reverse-transcribed into cdna. complementary cdna strands were annealed and the termini of the duplexes completed using dna polymerase i. full-length dna copies of rna segments 7, 8 and 9 were cloned into the pst i site of pbr322 and a clone containing the entire gene 7 was identified and sequenced. gene 7 is 1059 nucleotides in length and contains a single long open reading frame capable of ...	1983	6304629
serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence.	cloned dna copies of the double-stranded rna genomic segments of simian 11 rotavirus have been used to determine the coding assignment for vp7, the type-specific antigen of this virus. translation of hybrid-selected mrnas in an in vitro system supplemented with canine pancreatic microsomes permitted vp7 to be assigned to segment 9 and the two nonstructural viral proteins ncvp4 and ncvp3, to segments 7 and 8, respectively. hybridization of cloned dna probes for segments 7-9 with the corresponding ...	1983	6304692
factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus sa11.	a number of factors that affect genetic interaction during mixed infection with temperature-sensitive mutants of simian rotavirus sa11 have been examined. (1) statistical analyses of recombination frequency (rf) indicated that (a) the variability noted in rf was not related to variations in experimental conditions and (b) a linear map of the mutations could not be drawn. (2) the wild phenotype of recombinant progeny was stable on passage. (3) aggregates of progeny virus or heterozygous progeny v ...	1983	6305020
prevalence of rotavirus antibody in indian buffaloes and cattle.		1983	6305063
[studies on the use of staphylococcus protein a solid phase immune electron microscopy to increase the detection rate of rotavirus].		1983	6305465
[immunoelectron microscopic detection of viruses in newborn infants with acute gastroenteritis].		1983	6305518
epidemiology of human rotaviruses as determined by electrophoresis of genome rna.	an epidemiological survey was made of rotavirus infection in a children's hospital by determining changes in rotaviral genome patterns by means of polyacrylamide gel electrophoresis. in 180 fecal samples collected between december 1978 and june 1981, 53 distinct patterns were obtained belonging to 13 different types. two of these patterns dominated in endemic nosocomial infections of neonates until 1981, when one of them was replaced by a third pattern. two epidemics of diarrhea in the neonatal ...	1983	6305649
age-specific prevalence of antibody to rotavirus, escherichia coli heat-labile enterotoxin, norwalk virus, and hepatitis a virus in a rural community in thailand.	a serological survey in rural thailand demonstrated that inhabitants acquired antibody to rotavirus between the ages of 6 months and 6 years, to norwalk virus between the ages of 4 and 5 years, and to hepatitis a between the ages of 6 and 35 years. antibody of escherichia coli heat-labile toxin was most prevalent between 1 and 4 years and 18 and 25 years of age.	1983	6306049
replication of human rotavirus in cell culture.	nine strains of human rotavirus were adapted to growth in cv-1 and/or ma-104 cells following pretreatment of virus with trypsin, incorporation of trypsin into culture medium, and use of roller cultures. immunofluorescence was the most reliable method for the detection of virus replication, although characteristic cytopathic effects were produced sporadically by most isolates. virus could be readily detected in supernatant fluids of cell cultures and in cell sections by electron microscopy.	1983	6306163
prevalence of rotavirus in children in day care centers.		1983	6306197
capped and conserved terminal structures in human rotavirus genome double-stranded rna segments.	both 3'- and 5'-terminal structures of human rotavirus genome double-stranded rna segments were determined. rnas were labeled at the 3'-termini with [32p]pcp by incubation with rna ligase and at the 5'-termini with [32p]phosphate by polynucleotide kinase or, in the case of 5' caps, with 3h by chemical modification with [3h]nabh4. examination of radiolabeled termini released by digestion with several base-specific rnases revealed that rotavirus rna segments are base paired end-to-end and contain ...	1983	6306269
[viral enteritis in cattle].		1983	6306863
glycoprotein of human rotavirus wa strain grown in ma104 cells.		1983	6306865
aetiological studies on hospital in-patients with acute diarrhoea in calcutta.	bacterial enteropathogens and rotavirus were sought in 356 cases with acute diarrhoea admitted to the infectious diseases hospital, calcutta. one or more pathogens were isolated from 74.7% of the cases. single enteropathogens could be detected from 66% and multiple enteropathogens from 8.7% of the patients. vibrio cholerae biotype el tor, rotavirus, v. parahaemolyticus, and enteropathogenic and enterotoxigenic escherichia coli were the major pathogens detected. rotavirus was detected from 7.6% o ...	1983	6306873
primary isolation of cytopathic bovine rotaviruses on fetal rhesus monkey kidney cells.	primary isolation of bovine rotaviruses was successfully performed on rolling cultures of ma104 cells following trypsin treatment of fecal samples and cells. fifty-one fecal samples were obtained from 22 herds affected with naturally-occurring acute diarrhea in calves during a period of over two years. rotavirus particles were demonstrated in only 10 fecal samples by electron microscopy. fourteen cytopathic bovine rotaviruses were isolated from positive samples and could be serially cultivated o ...	1983	6306900
[enzyme-linked immunosorbent assay (elisa) in rotavirus detection].	reagents were obtained by means of which the method of the enzyme-linked immunosorbent assay (elisa) was worked out, based on the double sandwich principle, for the demonstration of rotavirus antigen presence in the fecal samples. the reaction makes use of polyvinylchloride plaques that are employed in tablet packing, and enables the rapid quantitative diagnosis of rotaviral enteritis in both animals and humans. the specificity of elisa has been demonstrated through the comparative investigation ...	1983	6306905
two types of glycoprotein precursors are produced by the simian rotavirus sa11.	the rotavirus genome codes for two glycoproteins: an outer capsid structural glycoprotein (vp7, apparent molecular weight 38,000 (38k)) and a nonstructural glycoprotein (ns28k). the synthesis of these glycoproteins was analyzed in infected cells and in a cell-free system derived from rabbit reticulocyte lysates supplemented with dog pancreatic microsomes. the data showed a 37k product synthesized in the cell-free system is the precursor to the 38k glycoprotein and that the 37k polypeptide contai ...	1983	6306912
dose effect on experimental reproduction of rotavirus diarrhoea in colostrum-deprived newborn calves.		1983	6302970
molecular biology of rotaviruses. v. terminal structure of viral rna species.	the terminal structure of rotavirus genomic ds rna and in vitro transcribed mrna was investigated using s1 nuclease digestion and 3' terminal sequence analysis. s1 nuclease analysis indicated that viral mrna was not a truncated copy of the genome rna at its 5' end. the 3' terminal sequence analysis of genomic plus strands and mrna showed that they were coterminal indicating that in vitro transcribed mrna is a full length copy of the genomic template. the sequence analysis performed on isolated v ...	1983	6302982
concentration of simian rotavirus sa-11 from tap water by membrane filtration and organic flocculation.	simian rotavirus sa-11 was concentrated from tap water by adsorption to and elution from microporous filters, followed by organic flocculation. two types of filters were compared for their ability to concentrate the virus. both zeta plus 60s and cox aa type m-780 filters were efficient for virus adsorption, but the efficiency of virus elution was higher with zeta plus than with cox filters. optimum conditions for virus recovery from zeta plus filters included an input water ph of 6.5 to 7.5 and ...	1983	6303220
[technic of preparing immune sera for rotavirus diagnosis].		1983	6303243
experiences connected with the use of elisa for technique rotavirus antigens and antibodies.		1983	6303244
[elaboration of a technic for the detection of rotaviruses in sewage].		1983	6303245
[comparative studies using the immunofluorescence technic in experimental transmissible gastroenteritis and rotavirus infection of piglets].		1983	6303246
[immunohistological detection of rotavirus antigen in the intestinal epithelium of children and adults].		1983	6303247
[electron-optic demonstration of rotaviruses in human small intestine epithelial cells].		1983	6303248
[model studies of the infectivity of aerosolized rotaviruses].		1983	6303249
[long-term study of the etiology of viral gastroenteritis in a children's institution].		1983	6303250
[cultivation of the human rotavirus].		1983	6303252