Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| overexpression of sod2 increases salt tolerance of arabidopsis. | the yeast (schizosaccharomyces pombe) sod2 (sodium2) gene was introduced into arabidopsis under the control of the cauliflower mosaic virus 35s promoter. transformants were selected for their ability to grow on medium containing kanamycin. southern- and northern-blot analyses confirmed that sod2 was transferred into the arabidopsis genome. there were no obvious morphological or developmental differences between the transgenic and wild-type (wt) plants. several transgenic homozygous lines and wt ... | 2003 | 14630955 |
| methanol production is enhanced by expression of an aspergillus niger pectin methylesterase in tobacco cells. | tobacco suspension culture cell (nicotiana tabacum, by2) was transformed with an aspergillus niger pectin methylesterase (pme; ec 3.1.1.11) cdna under the control of cauliflower mosaic virus (camv) 35s promoter. the transformant indicated a significant rise of pme and the level of methanol in the transformant increased by 28.7% compared to the vector control transformant. this is the first report of methanol overproduction in plant cells by means of genetic engineering. | 2003 | 14636709 |
| decreased content of leaf ferredoxin changes electron distribution and limits photosynthesis in transgenic potato plants. | a complete ferredoxin (fd) cdna clone was isolated from potato (solanum tuberosum l. cv desiree) leaves. by molecular and immunoblot analysis, the gene was identified as the leaf-specific fd isoform i. transgenic potato plants were constructed by introducing the homologous potato fed 1 cdna clone as an antisense construct under the control of the constitutive cauliflower mosaic virus 35s promoter. stable antisense lines with fd contents between 40% and 80% of the wild-type level were selected by ... | 2003 | 14645726 |
| transgenic expression of the arabidopsis della proteins gai and gai confers altered gibberellin response in tobacco. | bioactive gibberellin (ga) regulates the growth and development of a wide array of plant species. ga exerts its effects via members of the della protein family of putative transcriptional regulators. the gai gene encodes gai, a della protein from arabidopsis thaliana (l.) heyhn. a mutant allele, gai, encodes a mutant protein (gai) that has altered properties, and confers a dominant, reduced ga-response, dwarf phenotype. here we describe experiments to investigate the effects of transgenic expres ... | 2003 | 14713199 |
| salicylic acid and the hypersensitive response initiate distinct signal transduction pathways in tobacco that converge on the as-1-like element of the pr-1a promoter. | tobacco pathogenesis-related protein 1a (pr-1a) is induced in plants during the hypersensitive response (hr) after exposure of plants to salicylic acid (sa) and by developmental cues. gene activation by these diverse stimuli is mediated via an as-1-like element in the pr-1a upstream region. to further analyze the significance of this cis-acting sequence, an authentic as-1 element from the cauliflower mosaic virus 35s rna promoter was inserted into the pr-1a promoter in place of the as-1-like mot ... | 2003 | 14653814 |
| comparison of hcmv immediate early and camv 35s promoters in both plant and human cells. | cauliflower mosaic virus 35s promoter, widely used in transgenic crop plants, is known to be recognized in widely differing kinds of cells. its activity in human cells may have impact on the risk assessment for the environmental release of genetically modified plants. in this study, transient expression of several constructs containing beta-glucuronidase (gus) gene driven by cauliflower mosaic virus 35s promoter or by immediate early promoter of human cytomegalovirus (pcmv) was tested in both po ... | 2003 | 12890606 |
| a promoter derived from taro bacilliform badnavirus drives strong expression in transgenic banana and tobacco plants. | taro bacilliform virus (tabv) is a pararetrovirus of the genus badnavirus which infects the monocotyledonous plant, taro ( colocasia esculenta). a region of the tabv genome spanning nucleotides 6,281 to 12 (t1200), including the 3' end of open reading frame 3 (orf 3) and the intergenic region to the end of the trna(met)-binding site, was tested for promoter activity along with four different 5' deletion fragments (t600, t500, t250 and t100). in transient assays, only the t1200, t600, t500 fragme ... | 2003 | 12910370 |
| [construction of a plant expression vector with tomato transcription factor gene pti5 and studies on transgenic tobaccos]. | the plasmid pbi121uch1 carrying pti5-vp16 gene under the control of the cauliflower mosaic virus 35s promoter was constructed. leaf segments of tobacco sri were infected by agrobacterium tumefaciens eha105 with plasmid pbi121uch1, from which kanamycin resistant plants were obtained. pcr and southern analysis proved that the pti5-vp16 gene was integrated into the genomes of the tobacco plants. the disease resistance assay showed that the disease resistance was enhanced in the transgenic tobacco p ... | 2003 | 15639931 |
| stable and unstable mutations in the 5' non-translated regions of tomato aspermy virus rnas 1 and 2 generated de novo from infectious cdna clones containing a cauliflower mosaic virus 35s promoter. | tomato aspermy virus rnas derived from infectious cdna clones exhibited a number of sequence alterations in the 5' non-translated region (ntr). these included a deletion of the first four residues in both rnas 1 and 2, transversion of residue 5 from a g to a u in rna 1, and transversion of a to c at position of 50 of rna 1. these alterations were not stable in the infected plants while the insertion of a u residue between nucleotides 1 and 5 of rna 1 was stable in the infected plants. generation ... | 2004 | 15266109 |
| transgenic pinus radiata from agrobacterium tumefaciens-mediated transformation of cotyledons. | a method for agrobacterium tumefaciens-mediated transformation of pinus radiata cotyledon explants was developed using commercially available open-pollinated seed. pinus radiata is the most widely planted commercial conifer species in the southern hemisphere. reports on transformation of this species have relied on particle bombardment of embryogenic callus derived from immature embryos. the main drawback to the method is the small number of genotypes that are amenable to transformation and rege ... | 2004 | 14986058 |
| eucaryotic initiation factor 4b controls eif3-mediated ribosomal entry of viral reinitiation factor. | the cauliflower mosaic virus reinitiation factor tav interacts with host translation initiation factor 3 (eif3) and the 60s ribosomal subunit to accomplish translation of polycistronic mrnas. interaction between tav and eif3g is critical for the reinitiation process. here, we show that eif4b can preclude formation of the tav/eif3 complex via competition with tav for eif3g binding; indeed, the eif4b- and tav-binding sites on eif3g overlap. our data indicate that eif4b interferes with tav/eif3/40s ... | 2004 | 14988734 |
| rapid analysis of genetically modified organisms by in-house developed capillary electrophoresis chip and laser-induced fluorescence system. | a microfabricated, inexpensive, reusable glass capillary electrophoresis chip and a laser-induced fluorescence system were developed in-house for the rapid dna-based analysis of genetically modified organisms (gmos). the 35s promoter sequence of cauliflower mosaic virus and the terminator of the nopaline synthase (nos) gene from agrobacterium tumefaciens were both detected since they are present in most genetically modified organisms. the detection of genetically modified soybean in the presence ... | 2004 | 15004856 |
| altered susceptibility to infection by sinorhizobium meliloti and nectria haematococca in alfalfa roots with altered cell cycle. | most infections of plant roots are initiated in the region of elongation; the mechanism for this tissue-specific localization pattern is unknown. in alfalfa expressing psugt1 antisense mrna under the control of the cauliflower mosaic virus (camv) 35s promoter, the cell cycle in roots is completed in 48 h instead of 24 h, and border cell number is decreased by more than 99%. these plants were found to exhibit increased root-tip infection by a fungal pathogen and reduced nodule formation by a bact ... | 2004 | 15042410 |
| altered activity of the p2 isoform of plastidic glucose 6-phosphate dehydrogenase in tobacco (nicotiana tabacum cv. samsun) causes changes in carbohydrate metabolism and response to oxidative stress in leaves. | expression of one specific isoform of plastidic glucose 6-phosphate dehydrogenase (g6pdh) was manipulated in transgenic tobacco. antisense and sense constructs of the endogenous p2 form of g6pdh were used to transform plants under the control of the cauliflower mosaic virus (camv) 35s promotor. recombinant plants with altered expression were taken through to homozygosity by selective screening. northern analyses revealed substantial changes in the expression of the p2 form of g6pdh, with no appa ... | 2004 | 15053759 |
| overexpression of a cyanobacterial phosphoenolpyruvate carboxylase with diminished sensitivity to feedback inhibition in arabidopsis changes amino acid metabolism. | phosphoenolpyruvate carboxylase (ec 4.1.1.31) from synechococcus vulcanus (svpepc) is a unique enzyme, being almost insensitive to feedback inhibition at neutral ph. in order to assess its usefulness in metabolic engineering of plants, svpepc was expressed in arabidopsis thaliana (l.) heynh. under the control of the cauliflower mosaic virus 35s promoter. about one-third of the transformants of the t1 generation showed severe visible phenotypes such as leaf bleaching and were infertile when grown ... | 2004 | 15054659 |
| identification of the minimal sequence required for vascular-specific activity of tomato mottle taino virus replication-associated protein promoter in transgenic plants. | a 597 nt fragment from tomato mottle taino virus (tomotv) dna-a, with 459 nt located upstream of the replication-associated protein translation start codon, was tested for promoter activity in solanaceous plants. the promoter activity of this fragment (prep(459::rep)) was demonstrated when it was introduced upstream the uida reporter gene into tobacco, potato and tomato plants by genetic transformation. it became active in 7-day-old transgenic tobacco seedlings as revealed by a vascular-specific ... | 2004 | 15084394 |
| the avirulence domain of cauliflower mosaic virus transactivator/viroplasmin is a determinant of viral virulence in susceptible hosts. | cauliflower mosaic virus (camv) transactivator/viroplasmin (tav) is a multifunctional protein essential for basic replication of camv. it also plays a role in viral pathogenesis in crucifer and solanaceous host plants. deletion mutagenesis revealed that n- and c-terminal parts of tav are not essential for camv replication in transfected protoplasts. two deletion mutants having only minimal defects in basic replication were infectious in turnips but only with highly attenuated virulence. this was ... | 2004 | 15141951 |
| cloning and functional characterization of a phospholipid:diacylglycerol acyltransferase from arabidopsis. | a new pathway for triacylglycerol biosynthesis involving a phospholipid:diacylglycerol acyltransferase (pdat) was recently described (dahlqvist a, stahl u, lenman m, banas a, lee m, sandager l, ronne h, stymne s, [2000] proc natl acad sci usa 97: 6487-6492). the lro1 gene that encodes the pdat was identified in yeast (saccharomyces cerevisiae) and shown to have homology with animal lecithin:cholesterol acyltransferase. a search of the arabidopsis genome database identified the protein encoded by ... | 2004 | 15247387 |
| stable high-level transgene expression in arabidopsis thaliana using gene silencing mutants and matrix attachment regions. | basic and applied research involving transgenic plants often requires consistent high-level expression of transgenes. however, high inter-transformant variability of transgene expression caused by various phenomena, including gene silencing, is frequently observed. here, we show that stable, high-level transgene expression is obtained using arabidopsis thaliana post-transcriptional gene silencing (ptgs) sgs2 and sgs3 mutants. in populations of first generation (t1) a. thaliana plants transformed ... | 2004 | 15255872 |
| control of translation reinitiation on the cauliflower mosaic virus (camv) polycistronic rna. | translation of the polycistronic 35s rna of camv (cauliflower mosaic virus) occurs via a reinitiation mechanism, which requires tav (transactivator/viroplasmin). to allow translation reinitiation of the major open reading frames on the polycistronic rna, tav interacts with the host translational machinery via eif3 (eukaryotic initiation factor 3) and the 60s ribosome. accumulation of tav and eif3 in the polysomal fraction isolated from camv-infected cells suggested that tav prevents loss of eif3 ... | 2004 | 15270684 |
| ecdysone agonist-inducible expression of a coat protein gene from tobacco mosaic virus confers viral resistance in transgenic arabidopsis. | constitutive expression of a gene encoding tobacco mosaic virus (tmv) coat protein (cp) in transgenic plants confers resistance to infection by tmv and related tobamoviruses. here, we examined resistance to tmv by temporal and quantitative control of tmv cg cp (cgcp) gene expression using a simple, methoxyfenozide-inducible system in arabidopsis plants. by soil drenching with a commercial ecdysone agonist (intrepid-2f/methoxyfenozide), most transgenic lines were induced from undetectable levels ... | 2004 | 14731262 |
| an extracellular aspartic protease functions in arabidopsis disease resistance signaling. | we have used activation tagging with t-dna carrying cauliflower mosaic virus 35s enhancers to investigate the complex signaling networks underlying disease resistance in arabidopsis. from a screen of approximately 5000 lines, we identified constitutive disease resistance (cdr1) encoding an apoplastic aspartic protease, the overexpression of which causes dwarfing and resistance to virulent pseudomonas syringae. these phenotypes reflect salicylic-acid-dependent activation of micro-oxidative bursts ... | 2004 | 14765119 |
| untranslated regions from c4 amaranth ahrbcs1 mrnas confer translational enhancement and preferential bundle sheath cell expression in transgenic c4 flaveria bidentis. | many aspects of photosynthetic gene expression are posttranscriptionally regulated in c4 plants. to determine if rbcs mrna untranslated regions (utrs) in themselves could confer any characteristic c4 expression patterns, 5'- and 3'-utrs of ahrbcs1 mrna from the c4 dicot amaranth were linked to a gusa reporter gene. these were constitutively transcribed from a cauliflower mosaic virus promoter and assayed for posttranscriptional expression patterns in transgenic lines of the c4 dicot flaveria bid ... | 2004 | 15489276 |
| studies on the immunogenic potential of plant-expressed cholera toxin b subunit. | nicotiana tabacum var. samsun was transformed via agrobacterium-mediated transformation with a gene encoding the cholera toxin b subunit (ctb) of vibrio cholerae, modified to contain a sequence coding for an endoplasmic reticulum retention signal (sekdel), under the control of the cauliflower mosaic virus 35s promoter. total protein from the transgenic leaf tissue was isolated and an aliquot containing 5 microg recombinant ctb was injected intradermally into balb/c (h2k(d)) mice. ctb-specific se ... | 2004 | 14517689 |
| plant defense gene promoter enhances the reliability of shiva-1 gene-induced resistance to soft rot disease in potato. | pal5, a tomato (lycopersicon esculentum mill.) plant defense gene that encodes phenylalanine ammonia-lyase, is known to respond to a variety of environmental stresses including pathogen infection and wounding. a shiva-1 gene recombinant that encodes a small synthetic antibacterial peptide under the pal5 gene promoter was transformed into potato (solanum tuberosum l.) and its ability to induce resistance to erwinia carotovora was compared with a construct under the control of the constitutive and ... | 2004 | 15309537 |
| identification of a specific isoform of tomato lipoxygenase (tomloxc) involved in the generation of fatty acid-derived flavor compounds. | there are at least five lipoxygenases (tomloxa, tomloxb, tomloxc, tomloxd, and tomloxe) present in tomato (lycopersicon esculentum mill.) fruit, but their role in generation of fruit flavor volatiles has been unclear. to assess the physiological role of tomloxc in the generation of volatile c6 aldehyde and alcohol flavor compounds, we produced transgenic tomato plants with greatly reduced tomloxc using sense and antisense constructs under control of the cauliflower mosaic virus 35s promoter. the ... | 2004 | 15347800 |
| development of a highly efficient system for assessing recombinant gene expression in plant cell suspensions via agrobacterium tumefaciens transformation. | a transient gene-expression system was developed and used to characterize promoter strength, to verify suitability of bacterial gene modifications for expression in plant cells, and to express active antibody molecules. the system is based on suspension tobacco cells transformed by agrobacterium in a transient way. conditions such as pre-culture of tobacco cells and the co-cultivation period were identified as determinants to achieve high expression levels. under established conditions the activ ... | 2004 | 15154849 |
| spindly and gigantea interact and act in arabidopsis thaliana pathways involved in light responses, flowering, and rhythms in cotyledon movements. | spindly (spy) is a negative regulator of gibberellin signaling in arabidopsis thaliana that also functions in previously undefined pathways. the n terminus of spy contains a protein-protein interaction domain consisting of 10 tetratricopeptide repeats (tprs). gigantea (gi) was recovered from a yeast two-hybrid screen for proteins that interact with the tpr domain. gi and spy also interacted in escherichia coli and in vitro pull-down assays. the phenotypes of spy and spy-4 gi-2 plants support the ... | 2004 | 15155885 |
| larvicidal cry proteins from bacillus thuringiensis are released in root exudates of transgenic b. thuringiensis corn, potato, and rice but not of b. thuringiensis canola, cotton, and tobacco. | larvicidal proteins encoded by cry genes from bacillus thuringiensis were released in root exudates from transgenic b. thuringiensis corn, rice, and potato but not from b. thuringiensis canola, cotton, and tobacco. nonsterile soil and sterile hydroponic solution in which b. thuringiensis corn, rice, or potato had been grown were immunologically positive for the presence of the cry proteins; from b. thuringiensis corn and rice, the soil and solution were toxic to the larva of the tobacco hornworm ... | 2004 | 15191740 |
| oral administration of a mite allergen expressed by zucchini yellow mosaic virus in cucurbit species downregulates allergen-induced airway inflammation and ige synthesis. | sublingual-swallow immunotherapy in house dust mite-related asthma has a good safety profile and improves respiratory function and bronchial hyperreactivity. zucchini yellow mosaic virus (zymv) is envisaged as a promising viral vector for expressing large quantity of foreign proteins in cucurbit species. | 2004 | 15208588 |
| development of stem borer resistant transgenic parental lines involved in the production of hybrid rice. | stem borer resistant transgenic parental lines, involved in hybrid rice, were produced by agrobacterium-mediated gene transfer method. two psb111 super-binary vectors containing modified cry1ab/cry1ac genes driven by maize ubiquitin promoter, and herbicide resistance gene bar driven by cauliflower mosaic virus 35s promoter were, used in this study. embryogenic calli after co-cultivation with agrobacterium were selected on the medium containing phosphinothricin. southern blot analyses of primary ... | 2004 | 15219400 |
| quantitative promoter analysis in physcomitrella patens: a set of plant vectors activating gene expression within three orders of magnitude. | in addition to studies of plant gene function and developmental analyses, plant biotechnological use is largely dependent upon transgenic technologies. the moss physcomitrella patens has become an exciting model system for studying plant molecular processes due to an exceptionally high rate of nuclear gene targeting by homologous recombination compared with other plants. however, its use in transgenic approaches requires expression vectors that incorporate sufficiently strong promoters. to satis ... | 2004 | 15239842 |
| [increase in the level of proline and osmotic pressure of cytoplasm in transformed tobacco bearing an antisense suppressor of the proline dehydrogenase gene]. | the antisense suppressor was constructed for proline dehydrogenase gene (pdh; a fragment of pdh from arabidopsis in antisense orientation and under the control of 35s promoter of cauliflower mosaic virus, cmv). in nicotiana tabacum sr1 tobacco transformants bearing antisense suppressor for pdh, the proline content and the cytoplasm osmotic pressure were increased. the proline content in these transformants varied, whereas cytoplasm osmotic pressure was stable, which seems to reflect complicated ... | 2004 | 15065439 |
| manipulating volatile emission in tobacco leaves by expressing aspergillus nigerbeta-glucosidase in different subcellular compartments. | expression of the aspergillus nigerbeta-glucosidase gene, bgl1, in nicotiana tabacum plants (cv. xanthi) had a profound effect on the volatile emissions of intact and crushed leaves. bgl1 was expressed under the control of the cauliflower mosaic virus (camv) 35s promoter and targeted to the cytoplasm, cell wall, lytic vacuole (lv), chloroplast or endoplasmic reticulum (er). subcellular localization was confirmed by gold immunolabelling, followed by transmission electron microscopy (tem). signifi ... | 2004 | 17134395 |
| genetic manipulation of gamma-linolenic acid (gla) synthesis in a commercial variety of evening primrose (oenothera sp.). | a robust agrobacterium-mediated transformation procedure was developed for rigel, a commercial cultivar of evening primrose, and used to deliver a cdna encoding a delta(6)-desaturase from borage under the control of a cauliflower mosaic virus (camv) 35s promoter. analysis of the transformed plants demonstrated an altered profile of polyunsaturated fatty acids, with an increase in gamma-linolenic acid and octadecatetraenoic acid in leaf tissues when compared with control lines. | 2004 | 17134396 |
| differential roles of ac2 and ac4 of cassava geminiviruses in mediating synergism and suppression of posttranscriptional gene silencing. | posttranscriptional gene silencing (ptgs) in plants is a natural defense mechanism against virus infection. in mixed infections, virus synergism is proposed to result from suppression of the host defense mechanism by the viruses. synergistic severe mosaic disease caused by simultaneous infection with isolates of the cameroon strain of african cassava mosaic virus (acmv-[cm]) and east african cassava mosaic cameroon virus (eacmcv) in cassava and tobacco is characterized by a dramatic increase in ... | 2004 | 15308741 |
| a comparison of constitutive promoters for expression of transgenes in alfalfa (medicago sativa). | the activity of constitutive promoters was compared in transgenic alfalfa plants using two marker genes. three promoters, the 35s promoter from cauliflower mosaic virus (camv), the cassava vein mosaic virus (csvmv) promoter, and the sugarcane bacilliform badnavirus (scbv) promoter were each fused to the beta-glucuronidase (gusa) gene. the highest gus enzyme activity was obtained using the csvmv promoter and all alfalfa cells assayed by in situ staining had high levels of enzyme activity. the 35s ... | 2004 | 15517994 |
| transient expression in mammalian cells of transgenes transcribed from the cauliflower mosaic virus 35s promoter. | gene constructs containing the cauliflower mosaic virus (camv) 35s promoter and a sequence coding either for a green fluorescent protein (gfp) or for firefly luciferase were transfected into chinese hamster ovary (cho) cells. both reporter genes were expressed to significant levels. the 35s promoter was 40 times less active than the human ef1 alpha promoter, which is known to be one of the most potent promoters in mammalian cells. the 35s promoter must therefore be considered to be a promoter of ... | 2004 | 15612506 |
| a dnabeta associated with tomato yellow leaf curl china virus is required for symptom induction. | we report here that all 25 isolates of tomato yellow leaf curl china virus (tylccnv) collected from tobacco, tomato, or siegesbeckia orientalis plants in different regions of yunnan province, china, were associated with dnabeta molecules. to investigate the biological role of dnabeta, full-length infectious clones of viral dna and dnabeta of tylccnv isolate y10 (tylccnv-y10) were agroinoculated into nicotiana benthamiana, nicotiana glutinosa, nicotiana. tabacum samsun (nn or nn), tomato, and pet ... | 2004 | 15564504 |
| transgenic resistance to tobacco ringspot virus. | the coat protein (cp) gene including the 3'-untranslated region (utr) of rna2 of a cherry isolate of tobacco ringspot virus (trsv) was utilized in a cp-mediated resistance (cp-mr) strategy. to facilitate construction of plant expression vectors the sequence context of the cp gene translation initiation codon was modified at the 5'-end of the coding sequence by including an initiation codon. the gene was ligated to a version of the cauliflower mosaic virus (camv) 35s promoter with a duplicated en ... | 2004 | 15595207 |
| inappropriate annotation of a key defence marker in arabidopsis: will the real pr-1 please stand up? | pr-1 has been extensively used as a marker for salicylic acid (sa)-mediated defence and systemic and local acquired resistance. the arabidopsis genome project annotates at2g19990 as pr-1. this gene is also identified as pr-1 in two "full genome" arabidopsis microarrays, and tair cites approximately 60 articles to describe its patterns of expression. however, most of these citations are incorrect; the probes used were not at2g19990, but a homologous gene at2g14610, which is annotated as "pr-1-lik ... | 2004 | 15293050 |
| impact of altered gibberellin metabolism on biomass accumulation, lignin biosynthesis, and photosynthesis in transgenic tobacco plants. | gibberellins (gas) are involved in regulation of many aspects during plant development. to investigate the impact of altered ga levels on plant growth and metabolism, transgenic tobacco (nicotiana tabacum) plants have been engineered to express either a ga20-oxidase (atga20-ox) or a ga2-oxidase (atga2-ox) gene from arabidopsis under control of the cauliflower mosaic virus 35s promoter. resulting plants were characterized by elongated or stunted shoot growth, respectively, indicating changes in t ... | 2004 | 15122040 |
| activation of phenylpropanoid metabolism in sesame by over-expression of carrot calmodulin gene. | transgenic sesame (sesamum schinzianum asch.) was produced by agrobacterium-mediated transfection of a carrot calmodulin gene, cam-4, which was specifically expressed upon the contact of carrot cells with oligogalacturonide elicitor. coding region of cam-4 was ligated to the downstream of 35s promoter of cauliflower mosaic virus and subcloned into pmatgbo-db3.1. a. tumefaciens 4404 was transformed with the constructed vector, and the crown gall tissues formed in the sesame seedlings were transfe ... | 2004 | 15467207 |
| enhancer trapping identifies tri, an arabidopsis gene up-regulated by pathogen infection. | enhancer trap arabidopsis thaliana plants were screened for genes up-regulated by virus infection. the plants carried t-dna insertions comprising a minimal -60-bp cauliflower mosaic virus 35s promoter fused to the beta-glucuronidase (gus) reporter gene. approximately 12,000 plants were assayed for gus activity before and after rub-inoculation with tobacco rattle virus (trv) tagged with the green fluorescent protein (gfp). one plant and its progeny consistently showed upregulation of gus activity ... | 2004 | 15497401 |
| isolation and characterisation of three moss-derived beta-tubulin promoters suitable for recombinant expression. | the moss physcomitrella patens is an excellent tool to study plant gene-function relationships due to its high rate of homologous recombination (hr). it has also been shown to be very useful in the production of recombinant proteins which are secreted into a simple medium. thus, there is a need for suitable promoters functional in this well established model organism. we isolated genomic flanking regions of the beta-tubulin gene family from physcomitrella, concentrating on those family members s ... | 2004 | 15605251 |
| expression and characterization of hepatitis b surface antigen in transgenic potato plants. | transgenic potato plants expressing the gene of hepatitis b surface antigen (hbsag) under the control of the double promoter of 35s rna of cauliflower mosaic virus (camv 35ss) and the promoter of patatin gene of potato tubers have been obtained. biochemical analysis of the plants was performed. the amount of hbsag in leaves, microtubers, and tubers of transgenic potatoes growing in vitro and in vivo was 0.005-0.035% of the total soluble protein. hbsag content reached 1 microg/g in potato tubers ... | 2004 | 15527417 |
| characterization and activity enhancement of the phloem-specific pumpkin pp2 gene promoter. | the promoter of the pumpkin (cucurbita moschata) pp2 gene (designated np) was isolated from the restriction enzyme-digested genomic dna pool by genome walking and its activity and phloem specificity were examined in transgenic tobacco plants by using gus as a reporter. deletion analysis of the promoter revealed that the 473-bp fragment (-465 to + 8 relative to the transcription start site; designated as npii) exhibited similar activity as the full-length np promoter and retained its phloem speci ... | 2004 | 15672837 |
| induction of salt and osmotic stress tolerance by overexpression of an intracellular vesicle trafficking protein atrab7 (atrabg3e). | adaptation to stress requires removal of existing molecules from various cellular compartments and replacing them with new ones. the transport of materials to and from the specific compartments involved in the recycling and deposition of macromolecules is carried out by an intracellular vesicle trafficking system. here, we report the isolation of a vesicle trafficking-regulating gene, atrabg3e (formerly atrab7), from arabidopsis. the gene was induced during programmed cell death after treatment ... | 2004 | 14657401 |
| heterologous expression of the flavonoid 3',5'-hydroxylase gene of vinca major alters flower color in transgenic petunia hybrida. | flavonoid 3',5'-hydroxylase (f3'5'h) is the key enzyme for the expression of blue or purple flower color. a full-length cdna for the f3'5'h gene was cloned from petals of vinca major, and its genomic clone, designated vmfh1 (accession number ab078781 in the genbank/embl/ddbj databases), was isolated from leaves by a pcr-based strategy. nucleotide sequence analysis revealed that vmfh1 contains one intron and an open reading frame encoding a polypeptide of 506 amino acid residues. the deduced amin ... | 2004 | 14504908 |
| arabidopsis mutants that suppress the phenotype induced by transgene-mediated expression of cauliflower mosaic virus (camv) gene vi are less susceptible to camv-infection and show reduced ethylene sensitivity. | protein p6 is the main symptom determinant of cauliflower mosaic virus (camv), and transgene-mediated expression in arabidopsis induces a symptom-like phenotype in the absence of infection. seeds of a p6-transgenic line, a7, were mutagenized by gamma-irradiation and m2 seedlings were screened for mutants that suppressed the phenotype of chlorosis and stunting. we identified four mutants that were larger and less chlorotic than the a7 parent but which contained an intact and transcriptionally act ... | 2004 | 15604731 |
| level of glutathione is regulated by atp-dependent ligation of glutamate and cysteine through photosynthesis in arabidopsis thaliana: mechanism of strong interaction of light intensity with flowering. | glutathione (gsh) is associated with flowering in arabidopsis thaliana, but how gsh biosynthesis is regulated to control the transition to flowering remains to be elucidated. since the key reaction of gsh synthesis is catalyzed by gamma-glutamylcysteine synthetase (gamma-ecs) and all the gamma-ecs cdnas examined contained extra sequences for plastid targeting, we investigated the relationships among gsh levels, photosynthesis and flowering. the gsh level in arabidopsis increased with the light i ... | 2004 | 14749480 |
| gain-of-function and loss-of-function phenotypes of the protein phosphatase 2c hab1 reveal its role as a negative regulator of abscisic acid signalling. | hab1 was originally cloned on the basis of sequence homology to abi1 and abi2, and indeed, a multiple sequence alignment of 32 arabidopsis protein phosphatases type-2c (pp2cs) reveals a cluster composed by the four closely related proteins, abi1, abi2, hab1 and at1g17550 (here named hab2). characterisation of transgenic plants harbouring a transcriptional fusion prohab1: green fluorescent protein (gfp) indicates that hab1 is broadly expressed within the plant, including key target sites of absci ... | 2004 | 14731256 |
| preferential expression of a plant cystatin at nematode feeding sites confers resistance to meloidogyne incognita and globodera pallida. | the expression patterns of three promoters preferentially active in the roots of arabidopsis thaliana have been investigated in transgenic potato plants in response to plant parasitic nematode infection. promoter regions from the three genes, tub-1, arsk1 and rpl16a were linked to the gus reporter gene and histochemical staining was used to localize expression in potato roots in response to infection with both the potato cyst nematode, globodera pallida and the root-knot nematode, meloidogyne in ... | 2004 | 17166138 |
| overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic arabidopsis thaliana. | polyamines play pivotal roles in plant defense to environmental stresses. however, stress tolerance of genetically engineered plants for polyamine biosynthesis has been little examined so far. we cloned spermidine synthase cdna from cucurbita ficifolia and the gene was introduced to arabidopsis thaliana under the control of the cauliflower mosaic virus 35s promoter. the transgene was stably integrated and actively transcribed in the transgenic plants. as compared with the wild-type plants, the t ... | 2004 | 15215506 |
| localization of the n-terminal domain of cauliflower mosaic virus coat protein precursor. | cauliflower mosaic virus (camv) open reading frame (orf) iv encodes a coat protein precursor (pre-cp) harboring an n-terminal extension that is cleaved off by the camv-encoded protease. in transfected cells, pre-cp is present in the cytoplasm, while the processed form (p44) of cp is targeted to the nucleus, suggesting that the n-terminal extension might be involved in keeping the pre-cp in the cytoplasm for viral assembly. this study reports for the first time the intracellular localization of t ... | 2004 | 15207613 |
| activation tagging in plants: a tool for gene discovery. | a significant limitation of classical loss-of-function screens designed to dissect genetic pathways is that they rarely uncover genes that function redundantly, are compensated by alternative metabolic or regulatory circuits, or which have an additional role in early embryo or gametophyte development. activation t-dna tagging is one approach that has emerged in plants to help circumvent these potential problems. this technique utilises a t-dna sequence that contains four tandem copies of the cau ... | 2004 | 15156357 |
| wrinkled1 encodes an ap2/ereb domain protein involved in the control of storage compound biosynthesis in arabidopsis. | the accumulation of storage compounds during seed development ensures the survival of the young seedling, and also provides nutrition to humans and animals in the form of foods and feeds. the putative ap2/erebp transcription factor wrinkled1 (wri1) is involved in the regulation of seed storage metabolism in arabidopsis. a splicing mutant allele, wri1-1, caused the reduction of seed oil accumulation. glycolysis was compromised in this mutant, rendering developing embryos unable to efficiently con ... | 2004 | 15500472 |
| detection of genetically modified organisms by electrochemiluminescence pcr method. | with the development of biotechnology, more and more genetically modified organisms (gmos) have entered commercial market. because of the safety concerns, detection and characterization of gmos have attracted much attention recently. in this study, electrochemiluminescence polymerase chain reaction (ecl-pcr) combined with hybridization technique was applied to detect the gmos in genetically modified (gm) soybeans and papayas for the first time. whether the soybeans and the papayas contain gm com ... | 2004 | 15494222 |
| expression of the stilbene synthase (stsy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides. | when present, stilbene synthase leads to the production of resveratrol compounds, which are major components of the phytoalexin response against fungal pathogens of the plant and are highly bioactive substances of pharmaceutical interest. white poplar (populus alba l.) was transformed with a construct containing a cdna insert encoding stilbene synthase from grapevine (vitis vinifera l.), under the control of the cauliflower mosaic virus (camv) 35s promoter, and a chimeric kanamycin resistance ge ... | 2004 | 15359598 |
| overexpression of multiple dehydrin genes enhances tolerance to freezing stress in arabidopsis. | to elucidate the contribution of dehydrins (dhns) to freezing stress tolerance in arabidopsis, transgenic plants overexpressing multiple dhn genes were generated. chimeric double constructs for expression of rab18 and cor47 (ptp9) or lti29 and lti30 (ptp10) were made by fusing the coding sequences of the respective dhn genes to the cauliflower mosaic virus 35s promoter. overexpression of the chimeric genes in arabidopsis resulted in accumulation of the corresponding dehydrins to levels similar o ... | 2004 | 15356392 |
| overexpression of the arabidopsis 14-3-3 protein gf14 lambda in cotton leads to a "stay-green" phenotype and improves stress tolerance under moderate drought conditions. | the arabidopsis gene gf14 lambda that encodes a 14-3-3 protein was introduced into cotton plants to explore the physiological roles that gf14 lambda might play in plants. the expression level of gf14 lambda under the control of the cauliflower mosaic virus 35s promoter varied in transgenic cotton plants, and lines that expressed gf14 lambda demonstrated a "stay-green" phenotype and improved water-stress tolerance. these lines wilted less and maintained higher photosynthesis than segregated non-t ... | 2004 | 15356326 |
| the plant exon finder: a tool for precise detection of exons using a t-dna-based tagging approach. | we have developed a new tool, named the plant exon finder (pef), for identifying exons in plant genome sequences as an applied technique of t-dna insertional mutagenesis. the t-dna constructs contain a heat-shock gene promoter or the cauliflower mosaic virus (camv) 35s promoter, followed by the first exon of an arabidopsis gene with its start codon and the intron donor sequence facing the t-dna left border (lb) in order to trap exons in the genome. the constructs were used to make transgenic ara ... | 2004 | 15315830 |
| p6 protein of cauliflower mosaic virus, a translation reinitiator, interacts with ribosomal protein l13 from arabidopsis thaliana. | the p6 protein of cauliflower mosaic virus (camv) transactivates translation of the camv 35s polycistronic pregenomic rna and its spliced versions, and thus allows synthesis of a complete set of viral proteins. previous studies have shown that p6 interacts with plant l18 and l24 ribosomal proteins and initiation factor eif3, and it has been proposed that these interactions are involved in the reinitiation of translation of polycistronic viral rnas. this study characterizes a novel cellular partn ... | 2004 | 15557250 |
| the cauliflower mosaic virus 35s promoter extends into the transcribed region. | a 60-nucleotide region (s1) downstream of the transcription start site of the cauliflower mosaic virus 35s rna can enhance gene expression. by using transient expression assays with plant protoplasts, this activity was shown to be at least partially due to the effect of transcriptional enhancers within this region. we identify sequence motifs with enhancer function, which are normally masked by the powerful upstream enhancers of the 35s promoter. a repeated ct-rich motif is involved both in enha ... | 2004 | 15507598 |
| splicing of cauliflower mosaic virus 35s rna serves to downregulate a toxic gene product. | alternative splicing usually leads to an increase in the number of gene products that can be derived from a single transcript. here, a different and novel use of alternative splicing--as a means to control the amount of a potentially toxic gene product in the plant pararetrovirus cauliflower mosaic virus (camv)--is reported. about 70 % of the camv 35s rna, which serves as a substrate for both reverse transcription and polycistronic mrna, is spliced into four additional rna species. splicing occu ... | 2004 | 15302965 |
| a key factor of translation reinitiation, ribosomal protein l24, is involved in gynoecium development in arabidopsis. | in polycistronic genes, uorfs (upstream open reading frames) within the 5'-transcript leader sequence of major orfs may regulate the translation of these major orfs. in this case, ribosome reinitiates translation at a start codon of downstream orf after translation termination of uorf. the plant rpl24 (ribosomal protein l24) is a key factor for translation reinitiation of downstream orfs on the polycistronic cauliflower mosaic virus 35s rna transcription unit. in the rpl24-deficient mutant of ar ... | 2004 | 15270688 |
| activation of the oryza sativa non-symbiotic haemoglobin-2 promoter by the cytokinin-regulated transcription factor, arr1. | using in silico methods, several putative phytohormone-responsive cis-elements in the oryza sativa non-symbiotic haemoglobin (nshb) 1-4 and arabidopsis thaliana nshb1-2 promoters have been identified. an osnshb2 promoter::gus reporter gene fusion shows tissue-specific expression in a. thaliana. gus expression was observed in roots, the vasculature of young leaves, in flowers, and in the pedicel/stem junction. in transient assays, activity of the osnshb2 promoter was significantly up-regulated in ... | 2004 | 15258171 |
| level of accumulation of epoxy fatty acid in arabidopsis thaliana expressing a linoleic acid delta12-epoxygenase is influenced by the availability of the substrate linoleic acid. | arabidopsis thaliana (l.) heynh. expressing the crepis palaestina (l.) linoleic acid delta12-epoxygenase in its developing seeds typically accumulates low levels of vernolic acid (12,13-epoxy-octadec-cis-9-enoic acid) in comparison to levels found in seeds of the native c. palaestina. in order to determine some of the factors limiting the accumulation of this unusual fatty acid, we have examined the effects of increasing the availability of linoleic acid (9cis, 12cis-octadecadienoic acid), the s ... | 2004 | 15221145 |
| tomato heat stress transcription factor hsfb1 represents a novel type of general transcription coactivator with a histone-like motif interacting with the plant creb binding protein ortholog hac1. | in contrast with the class a heat stress transcription factors (hsfs) of plants, a considerable number of hsfs assigned to classes b and c have no evident function as transcription activators on their own. however, in the following article, we provide evidence that tomato (lycopersicon peruvianum) hsfb1 represents a novel type of coactivator cooperating with class a hsfs (e.g., with tomato hsfa1). provided the appropriate promoter architecture, the two hsfs assemble into an enhanceosome-like com ... | 2004 | 15131252 |
| the camv 35s promoter is highly active on floral organs and pollen of transgenic strawberry plants. | we have evaluated the expression of the reporter beta-glucuronidase (gus) gene driven by the cauliflower mosaic virus 35s (camv 35s) promoter in flowers and pollen from 14 independent transgenic strawberry lines. of the 14 lines evaluated, 13 (92.8%) showed gus activity--as estimated by the histochemical gus assay--in some floral organs, with expression being most common in the flower stem, sepals, petals, ovary and stigma. ten of these thirteen transgenic lines (77%) showed gus activity in poll ... | 2004 | 15048584 |
| effective production of marker-free transgenic strawberry plants using inducible site-specific recombination and a bifunctional selectable marker gene. | public concerns about the issue of the environmental safety of genetically modified plants have led to a demand for technologies allowing the production of transgenic plants without selectable (antibiotic resistance) markers. we describe the development of an effective transformation system for generating such marker-free transgenic plants, without the need for repeated transformation or sexual crossing. this system combines an inducible site-specific recombinase for the precise elimination of u ... | 2004 | 17147614 |
| predictable activation of tissue-specific expression from a single gene locus using the pop/lhg4 transactivation system in arabidopsis. | the pop/lhg4 transcription factor system was used to determine whether the synthetic pop promoter, integrated at one position in the arabidopsis genome, could be efficiently and faithfully activated by the heterologous transcription factor, lhg4, expressed in a variety of different patterns. this is a precondition for the development and exploitation of large collections of lhg4 activation lines that direct predictable tissue-specific expression of transgenes. we selected a pop-gus reporter inse ... | 2005 | 17168902 |
| antioxidant metabolite profiles in tomato fruit constitutively expressing the grapevine stilbene synthase gene. | tomato (lycopersicon esculentum mill.) tissues were transformed with a grape (vitis vinifera l.) stilbene synthase cdna, transcriptionally regulated by the cauliflower mosaic virus (camv) 35s promoter. transgenic plants accumulated new compounds, not present in either wild-type or vector-transformed plants. these were identified, by high-pressure liquid chromatography, as trans-resveratrol and trans-resveratrol-glucopyranoside. the amounts of trans-resveratrol and its piceid form were evaluated ... | 2005 | 17168899 |
| recombination every day: abundant recombination in a virus during a single multi-cellular host infection. | viral recombination can dramatically impact evolution and epidemiology. in viruses, the recombination rate depends on the frequency of genetic exchange between different viral genomes within an infected host cell and on the frequency at which such co-infections occur. while the recombination rate has been recently evaluated in experimentally co-infected cell cultures for several viruses, direct quantification at the most biologically significant level, that of a host infection, is still lacking. ... | 2005 | 15737066 |
| metabolic engineering of the mevalonate and non-mevalonate isopentenyl diphosphate-forming pathways for the production of health-promoting isoprenoids in tomato. | the genetic manipulation of both the mevalonic acid (mva) and methylerythritol-4-phosphate (mep) pathways, leading to the formation of isopentenyl diphosphate (ipp), has been achieved in tomato using 3-hydroxymethylglutaryl coa (hmgr-1) and 1-deoxy-d-xylulose-5-phosphate synthase (dxs) genes, respectively. transgenic plants containing an additional hmgr-1 from arabidopsis thaliana, under the control of the cauliflower mosaic virus (camv) 35s constitutive promoter, contained elevated phytosterols ... | 2005 | 17168896 |
| a single amino acid position in the helper component of cauliflower mosaic virus can change the spectrum of transmitting vector species. | viruses frequently use insect vectors to effect rapid spread through host populations. in plant viruses, vector transmission is the major mode of transmission, used by nearly 80% of species described to date. despite the importance of this phenomenon in epidemiology, the specificity of the virus-vector relationship is poorly understood at both the molecular and the evolutionary level, and very limited data are available on the precise viral protein motifs that control specificity. here, using th ... | 2005 | 16227279 |
| hahb-10, a sunflower homeobox-leucine zipper gene, is regulated by light quality and quantity, and promotes early flowering when expressed in arabidopsis. | homeodomain-leucine zipper proteins constitute a family of transcription factors found only in plants. expression patterns of the sunflower homeobox-leucine zipper gene hahb-10 (helianthus annuus homeobox-10), that belongs to the hd-zip ii subfamily, were analysed. northern blots showed that hahb-10 is expressed primarily in mature leaves, although expression is clearly detectable in younger leaves and also in stems. considerably higher expression levels were detected in etiolated seedlings comp ... | 2005 | 16215272 |
| hahb-4, a sunflower homeobox-leucine zipper gene, is a developmental regulator and confers drought tolerance to arabidopsis thaliana plants. | homeodomain-leucine zipper proteins constitute a family of transcription factors found only in plants. hahb-4 is a member of helianthus annuus (sunflower) subfamily i. it is regulated at the transcriptional level by water availability and abscisic acid. in order to establish if this gene plays a functional role in drought responses, transgenic arabidopsis thaliana plants that overexpress hahb-4 under the control of the 35s cauliflower mosaic virus promoter were obtained. transformed plants show ... | 2005 | 16201409 |
| class iii homeodomain leucine-zipper proteins regulate xylem cell differentiation. | although it has been suggested that class iii homeodomain leucine-zipper proteins (hd-zip iii) are involved in vascular development, details of the function of individual hd-zip iii proteins in vascular differentiation have not been resolved. to understand the function of each hd-zip iii protein in vascular differentiation precisely, we analyzed the in vitro transcriptional activity and in vivo function of zinnia hd-zip iii genes, zehb-10, zehb-11 and zehb-12, which show xylem-related expression ... | 2005 | 16081527 |
| overexpression of chloroplast-localized small molecular heat-shock protein enhances chilling tolerance in tomato plant. | there are many reports about the correlation between small molecular heat-shock protein (shsp) and the acquirement of chilling tolerance, but no direct evidence that shsp confers enhanced chilling tolerance to plant has been reported. a dna construct, including tomato chloroplast-localized small molecular heat-shock protein (cpshsp) cdna under the control of cauliflower mosaic virus 35s (35scamv) promoter, was introduced into the genome of tomato plants. the chilling tolerance of the transgenic ... | 2005 | 15840935 |
| enhanced radical scavenging activity of genetically modified arabidopsis seeds. | the proanthocyanidin (pa) content was increased in seeds of pap1-d mutant of arabidopsis thaliana, in which the expression of endogenous pap1 gene encoding a myb-like transcription factor was induced by activation-tagging with enhancer sequences derived from cauliflower mosaic virus 35s promoter. in contrast, the pa contents decreased in seeds of transgenic plants transformed with a pap1 cdna or with a pap1 chimeric repressor, although the amount of soluble anthocyanins increased in seeds of tra ... | 2005 | 15834789 |
| expression of arabidopsis phytochelatin synthase 2 is too low to complement an atpcs1-defective cad1-3 mutant. | phytochelatins play an important role in heavy metal detoxification in plants as well as in other organisms. the arabidopsis thaliana mutant cad1-3 does not produce detectable levels of phytochelatins in response to cadmium stress. the hypersensitivity of cad1-3 to cadmium stress is attributed to a mutation in the phytochelatin synthase 1 (atpcs1) gene. however, a. thaliana also contains a functional phytochelatin synthase 2 (atpcs2). in this study, we investigated why the cad1-3 mutant is hyper ... | 2005 | 15750344 |
| plants with increased expression of ent-kaurene oxidase are resistant to chemical inhibitors of this gibberellin biosynthesis enzyme. | the gibberellin (ga) biosynthetic pathway includes the three-step oxidation of ent-kaurene to ent-kaurenoic acid, catalyzed by the enzyme ent-kaurene oxidase (ko). arabidopsis plants overexpressing the ko cdna under the control of the cauliflower mosaic virus 35s promoter, with or without a translational fusion to a modified green fluorescent protein (gfp), are very similar to wild-type (wt) plants under normal growth conditions. in contrast, when wt and 35s:ko (or 35s:ko-gfp) seeds, seedlings o ... | 2005 | 15695465 |
| phototropin from chlamydomonas reinhardtii is functional in arabidopsis thaliana. | phototropin, a plant blue light photoreceptor, mediates important blue light responses such as phototropism, chloroplast positioning and stomatal opening in higher plants. in arabidopsis thaliana, two phototoropins, phototropin 1 and 2, are known. recently, in the unicellular green alga, chlamydomonas reinhardtii, a phototropin homolog was identified. it exhibits photochemical properties similar to those of higher plant phototropins and is involved in multiple steps of the sexual life cycle of c ... | 2005 | 15695460 |
| multiple protein regions contribute to differential activities of yabby proteins in reproductive development. | members of the yabby family of putative transcription factors participate in abaxial-adaxial identity determination in lateral organs in arabidopsis (arabidopsis thaliana). two yabby genes specifically expressed in reproductive structures, crabs claw (crc) and inner no outer (ino), have additional activities, with crc promoting nectary development and carpel fusion, and ino responding to spatial regulation by superman during ovule development. all yabby coding regions, except yabby5, were able t ... | 2005 | 15665244 |
| structure of the mature p3-virus particle complex of cauliflower mosaic virus revealed by cryo-electron microscopy. | the cauliflower mosaic virus (camv) has an icosahedral capsid composed of the viral protein p4. the viral product p3 is a multifunctional protein closely associated with the virus particle within host cells. the best-characterized function of p3 is its implication in camv plant-to-plant transmission by aphid vectors, involving a p3-virion complex. in this transmission process, the viral protein p2 attaches to virion-bound p3, and creates a molecular bridge between the virus and a putative recept ... | 2005 | 15663943 |
| abundant expression in vascular tissue of plant taf10, an orthologous gene for tata box-binding protein-associated factor 10, in flaveria trinervia and abnormal morphology of arabidopsis thaliana transformants on its overexpression. | taf10 is one of the tata box-binding protein-associated factors (tafs), which constitute the tfiid complex. we isolated a plant taf10 ortholog from a flaveria trinervia cdna library, and named it fttaf10. the fttaf10 polypeptide contains a histone-fold motif, which is highly conserved among the taf10s of other organisms. a transiently expressed green fluorescent protein (gfp) fusion protein was translocated into the nuclei of onion epidermal cells, suggesting that the fttaf10 functions in nuclei ... | 2005 | 15659449 |
| characterization of a member of the an subfamily, ian, from ipomoea nil. | angustifolia (an) is the first c-terminal binding protein (ctbp) gene from plants and controls leaf width and pattern of trichome branching in arabidopsis thaliana (l.) heynh. we characterized an ortholog of an from ipomoea nil (l.) roth (japanese morning glory) and designated it ipomoea nil's an (ian). ian is a single-copy gene in the genome and is expressed ubiquitously in various organs of i. nil. ian contains not only a d2-hdh motif, which is highly conserved within the ctbp family, but also ... | 2005 | 15659438 |
| cauliflower mosaic virus, a compatible pathogen of arabidopsis, engages three distinct defense-signaling pathways and activates rapid systemic generation of reactive oxygen species. | we analyzed expression of marker genes for three defense pathways during infection by cauliflower mosaic virus (camv), a compatible pathogen of arabidopsis (arabidopsis thaliana), using luciferase reporter transgenes and directly by measuring transcript abundance. expression of pr-1, a marker for salicylic acid signaling, was very low until 8 d postinoculation and then rose sharply, coinciding with the rise in virus levels. in contrast, as early as 2 h postinoculation, transcriptional up-regulat ... | 2005 | 16169957 |
| starch-related alpha-glucan/water dikinase is involved in the cold-induced development of freezing tolerance in arabidopsis. | cold-induced soluble sugar accumulation enhances the degree of freezing tolerance in various cold-hardy plants including arabidopsis (arabidopsis thaliana), where soluble sugars accumulate in only a few hours at 2 degrees c. hence, along with photosynthesis, starch degradation might play a significant role in cold-induced sugar accumulation and enhanced freezing tolerance. starch-related alpha-glucan/water dikinase (ec 2.7.9.4), encoded by arabidopsis starch excess 1 (sex1), is hypothesized to r ... | 2005 | 15894744 |
| a lily asr protein involves abscisic acid signaling and confers drought and salt resistance in arabidopsis. | lla23, an abscisic acid-, stress-, and ripening-induced protein, was previously isolated from lily (lilium longiflorum) pollen. the expression of lla23 is induced under the application of abscisic acid (aba), nacl, or dehydration. to provide evidence on the biological role of lla23 proteins against drought, we used an overexpression approach in arabidopsis (arabidopsis thaliana). constitutive overexpression of lla23 under the cauliflower mosaic virus 35s promoter confers reduced sensitivity to a ... | 2005 | 16169963 |
| antisense suppression of deoxyhypusine synthase in tomato delays fruit softening and alters growth and development. | the effects of suppressing deoxyhypusine synthase (dhs) have been examined in tomato (solanum lycopersicum cv uct5). dhs mediates the first of two sequential enzymatic reactions that activate eukaryotic translation initiation factor-5a (eif-5a) by converting a conserved lys to the unusual amino acid, deoxyhypusine. dhs protein levels were suppressed in transgenic plants by expressing the 3'-untranslated region of tomato dhs under regulation of the constitutive cauliflower mosaic virus promoter. ... | 2005 | 15951486 |
| plantacyanin plays a role in reproduction in arabidopsis. | plantacyanins belong to the phytocyanin family of blue copper proteins. in the arabidopsis (arabidopsis thaliana) genome, only one gene encodes plantacyanin. the t-dna-tagged mutant is a knockdown mutant that shows no visible phenotype. we used both promoter-beta-glucuronidase transgenic plants and immunolocalization to show that arabidopsis plantacyanin is expressed most highly in the inflorescence and, specifically, in the transmitting tract of the pistil. protein levels show a steep gradient ... | 2005 | 15908590 |
| immunopurification of polyribosomal complexes of arabidopsis for global analysis of gene expression. | immunoaffinity purification of polyribosomes (polysomes) from crude leaf extracts of arabidopsis (arabidopsis thaliana) was achieved with transgenic genotypes that overexpress a translational fusion of a ribosomal protein (rp) with a his(6)-flag dual epitope tag. in plants with a cauliflower mosaic virus 35s:hf-rpl18 transgene immunopurification with anti-flag agarose beads yielded 60-svedberg ribosomal subunits, intact 80-svedberg monosomes and polysomes. sucrose density gradient fractionation ... | 2005 | 15955926 |
| pea dna helicase 45 overexpression in tobacco confers high salinity tolerance without affecting yield. | salt tolerance is an important trait that is required to overcome salinity-induced reduction in plant productivity. we have reported previously the isolation of a pea dna helicase 45 (pdh45) that exhibits striking homology with the eukaryotic translation initiation factor eif-4a. here, we report that pdh45 mrna is induced in pea seedlings in response to high salt, and its overexpression driven by a constitutive cauliflower mosaic virus-(35)s promoter in tobacco plants confers salinity tolerance, ... | 2005 | 15630095 |
| hairpin rna-mediated silencing of plum pox virus p1 and hc-pro genes for efficient and predictable resistance to the virus. | we report the application of the hairpin-mediated rna silencing technology for obtaining resistance to plum pox virus (ppv) infection in nicotiana benthamiana plants. four sequences, covering the p1 and silencing suppressor hc-pro genes of an italian ppv m isolate, were introduced into n. benthamiana plants as two inverted repeats separated by an intron sequence under the transcriptional control of the cauliflower mosaic virus 35s promoter. in a leaf disk infection assay, 38 out of 40 t0 transge ... | 2005 | 16315100 |
| characteristics of the promoters derived from the single-stranded dna components of milk vetch dwarf virus in transgenic tobacco. | predicted promoter regions of milk vetch dwarf virus (mdv) components (c1-c11) were isolated and fused with a beta-glucuronidase (gus) reporter gene and the characteristics of the promoters were examined. in transgenic tobacco calli, promoters of mdv c4 (encoding a cell-cycle link protein), c5 and c7 (both encoding unknown proteins), c6 (encoding a nuclear-shuttle protein) and c8 (encoding a movement protein) generated a stronger level of gus expression than the cauliflower mosaic virus 35s rna ... | 2005 | 15914865 |
| oslsd1, a rice zinc finger protein, regulates programmed cell death and callus differentiation. | the arabidopsis lsd1 and lol1 proteins both contain three conserved zinc finger domains and have antagonistic effects on plant programmed cell death (pcd). in this study, a rice (oryza sativa) functional homolog of lsd1, designated oslsd1, was identified. the expression of oslsd1 was light-induced or dark-suppressed. overexpression of oslsd1 driven by the cauliflower mosaic virus 35s promoter accelerated callus differentiation in transformed rice tissues and increased chlorophyll b content in tr ... | 2005 | 15915636 |
| acclimation to diverse environmental stresses caused by a suppression of cytosolic ascorbate peroxidase in tobacco by-2 cells. | the active oxygen species (aos) that arise from normal metabolic processes are kept under tight control by various antioxidant mechanisms. aos are important signal molecules that regulate many physiological processes, including environmental stress responses. in this work, we have investigated the effect of lowered cytosolic ascorbate peroxidase (apx) activity in transgenic tobacco by-2 cells, using two transformed by-2 cell lines, capx-s2 and capx-s3, resulting from co-suppression by expression ... | 2005 | 15919670 |
| identification of functional apple scab resistance gene promoters. | apple scab (venturia inaequalis) is one of the most damaging diseases affecting commercial apple production. some wild malus species possess resistance against apple scab. one gene, hcrvf2, from a cluster of three genes derived from the wild apple malus floribunda clone 821, has recently been shown to confer resistance to apple scab when transferred into a scab-susceptible apple variety. for this proof-of-function experiment, the use of the 35s promoter from cauliflower mosaic virus was reliable ... | 2005 | 15726316 |