Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| specific inhibition of herpesvirus ribonucleotide reductase by synthetic peptides. | ribonucleotide reductase is an essential enzyme for dna synthesis in all prokaryotic and eukaryotic cells; it catalyses the reductive conversion of ribonucleotides to deoxyribonucleotides. several herpesviruses including herpes simplex virus type 1 (hsv-1), hsv-2, pseudorabies virus (prv), equine herpesvirus type 1 (ehv-1) and epstein-barr virus (ebv) have been found to induce novel ribonucleotide reductase activities. there is evidence that the hsv-1 ribonucleotide reductase activity is virus-e ... | 1986 | 3012359 |
| analysis of dna from recurrent genital herpes simplex virus isolates by restriction endonuclease digestion. | in a blind study, dna from 40 clinical isolates of herpes simplex viruses was analyzed by restriction endonucleases to determine whether serial isolates from an individual patient could be identified and whether exogenous reinfection occurred within this population. five of the 40 isolates served as controls. based on restriction patterns obtained following bam h1 cleavage of dna, 35 isolates were assigned to 15 patients. isolates from two patients displayed variation in the electrophoretic mobi ... | 1986 | 3012807 |
| cellular immunity to vaccinations and herpesvirus infections after bone marrow transplantation. | the cellular immune response to herpesviruses was studied in 46 recipients of marrow grafts (23 autologous, 23 allogeneic). that study was performed in vitro by evaluating the degree of lymphocyte proliferative responses to herpes simplex virus (hsv), cytomegalovirus (cmv), and varicella zoster virus (vzv). no primary infections with any of those viruses were noted after bone marrow transplantation (bmt). the incidence of active infection in seropositive patients was significantly lower after au ... | 1986 | 3012833 |
| efficacy of the acyclic guanosine analog buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] in experimental genital herpes. | the efficacy of the anti-herpesvirus drug buciclovir [(r)-9-(3,4-dihydroxybutyl)guanine] was investigated in guinea pigs and mice infected intravaginally with herpes simplex virus type 2. topical treatment initiated early after infection was efficacious, in contrast to topical treatment delayed 24 h or more. systemic treatment of infected mice could not prevent the spread of virus to the brain and mortality. systemically administered buciclovir had an effect in guinea pigs, even after delayed on ... | 1986 | 3013082 |
| comparison of susceptibilities of varicella-zoster virus and herpes simplex viruses to nucleoside analogs. | the susceptibilities of varicella-zoster virus (vzv) and herpes simplex virus type 1 (hsv-1) and type 2 (hsv-2) to 17 nucleoside analogs were compared by a plaque reduction assay with human embryonic lung fibroblast cells. the susceptibility of vzv to certain nucleoside analogs was different from that of hsv-1. against vzv the 5-halogenovinyl-arabinosyluracils were the most potent of the compounds tested. | 1986 | 3013088 |
| evaluation of five cell types for the isolation of herpes simplex virus. | five cells were evaluated in a comparative analysis for sensitivity, specificity, and rapidity in detecting the presence of herpes simplex virus hsv-1 and hsv-2. included in this study were human embryonic kidney (hek), rabbit kidney (rk), mrc-5, mink lung (ml), and microtus agrestis (umma). a total of 274 specimens from genital, throat, skin, or other sources that were submitted for hsv isolation were used in the study. the sensitivity of the different cells was assessed by the total number of ... | 1986 | 3013496 |
| rapid identification of herpesvirus simiae (b virus) dna from clinical isolates in nonhuman primate colonies. | a rapid, simple technique, based on restriction endonuclease analysis of radioactively labeled infected cell dna, is described for identification of herpesvirus simiae (b virus) infection in clinical isolates from nonhuman primates. isolates can be screened within 2-3 days from the time of collection of a specimen from a suspect lesion to final viral identification. isolates were obtained from eight animals with suspected b virus infections. the results indicated the presence of b virus in each ... | 1986 | 3013916 |
| a filamentous distribution for the herpes simplex virus type 2-encoded major dna-binding protein. | monoclonal antibodies reacting with the herpes simplex virus (hsv)-encoded major dna-binding protein defined an intracellular filamentous network. this network was associated predominantly with the infected cell nucleus and occurred in cells infected with hsv type 2. it did not co-distribute with microfilaments, microtubules or intermediate filaments, and dna synthesis was required for its formation. we suggest explanations for the occurrence and function of this novel filamentous network struct ... | 1986 | 3014046 |
| characterization of the tumor-associated 38-kd protein of herpes simplex virus type 2. | the bglii n dna fragment of herpes simplex virus type 2 (hsv 2), which is capable of oncogenically transforming cells in vitro, encodes a 37,800-dalton (38-kd) protein that has been seroepidemiologically associated with uterine cervical carcinoma. polyclonal monospecific antiserum was produced against electrophoretically purified 38 kd from hsv 2-infected cells and used to identify antigenic and biochemical characteristics of the protein as well as to probe transformed cells for the expression o ... | 1986 | 3014138 |
| comparative studies of host-cell reactivation, cellular capacity and enhanced reactivation of herpes simplex virus in normal, xeroderma pigmentosum and cockayne syndrome fibroblasts. | host-cell reactivation (hcr) of uv-irradiated herpes simplex virus type 2 (hsv-2), capacity of uv-irradiated cells to support hsv-2 plaque formation and uv-enhanced reactivation (uver) of uv-irradiated hsv-2 were examined in fibroblasts from 4 patients with cockayne syndrome (cs), 5 with xeroderma pigmentosum and 5 normals. all uv-survival curves for hsv-2 plaque formation showed 2 components. hcr was similar to normal for the xp variant strain and the 2 cs strains tested, but substantially redu ... | 1986 | 3014327 |
| [comparative study of the effectiveness of acycloguanosine and phosphonoacetic acid in herpetic infection in cell cultures]. | a comparative study of cytotoxicity and antiherpes activity of acycloguanosine (acg) of the soviet and american manufacture and a soviet preparation of phosphonoacetic acid (paa) for herpes simplex virus types 1 and 2 (hsv-1 and hsv-2) was carried out in primary and continuous cell cultures. the acg preparation was shown not to be inferior in maximal tolerated concentration, minimal inhibiting concentration, and chemotherapeutic index (cti) to american drug. zovirax (zvr), in relation to both he ... | 1986 | 3014751 |
| activities of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine and its metabolites against herpes simplex virus types 1 and 2 in cell culture and in mice infected intracerebrally with herpes simplex virus type 2. | as measured by plaque and yield reduction assays, several metabolites of 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac) were highly active against herpes simplex virus types 1 and 2. these metabolites included the 2'-deoxy-2'-fluoroarabinosyl derivatives of 5-iodouracil (fiau), cytosine (fac), uracil (fau), and thymine (fmau). in mice inoculated intracerebrally with herpes simplex virus type 2, the relative order of potency of these compounds and licensed antiviral drugs was ... | 1986 | 3015003 |
| effect of interferon on herpes simplex virus replication in murine macrophage-like cell lines. | the effect of interferon on the replication of herpes simplex virus types 1 and 2 was studied in two murine macrophage-like cell lines which differ in their ability to synthesize interferon. higher titers of herpes simplex virus type 1 occurred in pu5-1.8 cell cultures where interferon was not produced than in j774a.1 cell cultures where low amounts of interferon were produced. herpes simplex virus type 2 replicated poorly in both types of cell cultures. interferon synthesis was induced in j774a ... | 1986 | 3015020 |
| herpes simplex virus type 1 and 2 in the adrenal glands: replication and histopathology. | the adrenal glands were shown to be the most severely infected organs in the early phase of hsv-1 infections (up to 10 days p.i.) after i.p. infections in mice. virus could be isolated from the adrenal glands as early as one hour after infection with pathogenic and apathogenic strains. infection of the adrenal glands is a result of viremia. the content of hsv-1 (5 strains) was much higher in the adrenals than in spleen and liver. it peaked at 3-4 days p.i. compared to 1-2 days in spleen and live ... | 1986 | 3015081 |
| a latent infection of herpes simplex virus type 2 in a human neuroblastoma cell line imr-32. | human neuroblastoma (imr-32) cells were infected with herpes simplex virus type 2 (hsv-2) at a multiplicity of infection (moi) of 2 plaque-forming units (pfu)/cell and were cultured at 40 degrees c for 14 days. then neither infectious virus particles nor virus capsids were detected in these cells whereas the presence of virus-specific antigens was observed by immunofluorescent antibody staining technique in 16.9 +/- 3.2 per cent of the infected cell population. when the cultivation temperature w ... | 1986 | 3015082 |
| transformation of dog embryo kidney cells by human herpesviruses. | the infection of dog embryo kidney (dek) cells with herpes simplex virus type 2 (hsv-2) or human cytomegalovirus (hcmv) led to the development of transformed cell lines. rapidly dividing dek cells with unlimited division potential exhibited growth in 2% serum, contained nuclear virus antigens, and formed small (+/- 0.2 mm) colonies in 0.3% agarose. immortal cell lines showing the same transformation properties were also obtained after transfection with purified hsv-2 or hcmv dna. these results c ... | 1986 | 3015422 |
| identification, properties, and gene location of a novel glycoprotein specified by herpes simplex virus 1. | we report the identification of a novel herpes simplex virus 1 (hsv-1) glycoprotein reactive with type specific monoclonal antibody h1379. the monoclonal antibody reacted with two broad bands with apparent mol wt of 60k to 68k and 44k to 48k formed by infected cell lysates subjected to electrophoresis in denaturing polyacrylamide gels and electrically transferred to a nitrocellulose sheet. early in infection the h1379 reactive protein was found in the faster migrating band. the rate of accumulat ... | 1986 | 3006335 |
| comparison of risk factors for cervical cancer in different populations. | the incidence of cervical cancer has been found to vary between populations. risk factors of cervical cancer include early age at first marriage, multiple marriages and antibodies to herpes simplex virus type 2 (hsv-2). the interrelatedness of these risk factors was examined by comparing data collected from 428 cancer cases and 947 control women selected from 6 populations having standardized cervical cancer incidence rates varying from 9.3 to 85.1 per 100,000. logistic regression analysis revea ... | 1986 | 3007372 |
| characterization of a herpes simplex virus type 2-specified glycoprotein with affinity for n-acetylgalactosamine-specific lectins and its identification as g92k or gg. | extracts from herpes simplex virus type 2 (hsv-2)-infected cells were subjected to affinity chromatography with gel-bound helix pomatia lectin (hpa). only one hsv-2-specified glycoprotein was isolated by this procedure and the glycoprotein had an apparent molecular weight of 130 000 (130k). the hpa-binding glycoprotein was genetically mapped, using hsv-1 x hsv-2 intertypic recombinants into the short component of the hsv-2 genome. the mapping position, electrophoretic mobility and the antigenic ... | 1986 | 3007660 |
| novel herpes simplex virus type 1 glycoproteins identified by antiserum against a synthetic oligopeptide from the predicted product of gene us4. | gene us4 of herpes simplex virus type 1 (hsv-1) has been predicted, from dna sequence analysis, to encode a protein of molecular weight 25237 and its properties suggest it to be a membrane-associated protein. we have investigated this protein by raising antiserum to a synthetic oligopeptide corresponding to a stretch of amino acids from an internal hydrophilic region of the predicted sequence. this antiserum immunoprecipitates three glycoprotein species of apparent mol. wt. 37 000, 48 000 and 56 ... | 1986 | 3007661 |
| herpes simplex virus type 1 dna sequences which direct spread of virus from cornea to central nervous system. | the virulence of a herpes simplex virus (hsv) intertypic recombinant possessing hsv-1 dna sequences from map units 0.31 to 0.44 and hsv-2 sequences from map units 0 to 0.30 and 0.45 to 1.0 were compared with the virulence of the two parental strains. following ocular inoculation, both the intertypic recombinant and the hsv-1 parent replicated at the infection site and spread to the peripheral and central nervous system (cns) to produce fatal encephalitis. the hsv-2 parent also replicated at the ... | 1986 | 3008422 |
| efficacy of 5-methoxymethyl-2'-deoxyuridine in combination with arabinosyladenine for the treatment of primary herpes simplex genital infection of mice and guinea pigs. | the relative efficacy of 5-methoxymethyl-2'-deoxyuridine (mmdurd), arabinosyladenine (ara-a) and the combination of mmdurd and ara-a in the treatment of experimental genital herpes (gh) was investigated using mouse and guinea pig models. the infection was initiated by intravaginal inoculation using either hsv-2, strain x-265 or hsv-2, strain ms. treatment was initiated 3 h post virus inoculation. the parameters used to evaluate efficacy were: percent mortality; mean day of death; virus yield fro ... | 1986 | 3008644 |
| antibody response to the herpes simplex virus type 2 ag-4 antigen in guinea pigs with genital herpes infections. | female guinea pigs, inoculated intravaginally with a herpes simplex virus-type 2 (hsv-2) strain m1, developed typical symptoms of a primary and recurrent genital herpes infection. sera from hsv-2 infected guinea pigs taken during the primary or recurrent stages of the genital infection contained complement (c)-fixing antibody which reacted with an apparent type specificity to an early 4 h hsv-2 infected cell extract (ag-4) when compared to a 4 h hsv-1 infected cell extract. this c-fixing anti-ag ... | 1986 | 3008691 |
| typing of herpes simplex virus with synthetic dna probes. | three single-stranded oligonucleotide probes, 22 bases long, homologous to unique regions of herpes simplex virus (hsv) types 1 (hsv-1) and 2 (hsv-2) and a region common to both were chemically synthesized with use of a modified phosphochloridite protocol. for hybridization experiments each probe was labeled with use of polynucleotide kinase and [gamma-32p] atp to a specific activity of approximately 2 x 10(9) cpm/micrograms. two hundred one clinical isolates of hsv (96 hsv-1 and 105 hsv-2) coll ... | 1986 | 3005436 |
| immunological reactivity of human sera with individual herpes simplex proteins: a comparative study of sera from patients with preinvasive or invasive cervical cancer and from controls. | forty-three human sera collected from patients with preinvasive or invasive cervical carcinoma were analyzed for their repertoire of herpes simplex virus (hsv) specific antibodies reactive with individual viral hsv-1 and hsv-2 proteins. the reactivity was compared to that of sera from 27 control persons. the patients and controls were clinically and histologically characterized in a previous study we carried out, where the analysis of the hla-antigen pattern was compared among the groups [vass-s ... | 1986 | 3005489 |
| characterization of the genes encoding herpes simplex virus type 1 and type 2 alkaline exonucleases and overlapping proteins. | a detailed sequence analysis of the herpes simplex virus type 1 (hsv-1) and hsv-2 dna encoding the alkaline exonuclease mrna clusters has been completed. three partially colinear mrnas (2.3, 1.9, and 0.9 kilobases) are completely encoded within the dna sequence presented. the putative promoter regions of the transcripts were inserted upstream of a plasmid-borne chloramphenicol acetyl transferase (cat) gene and assayed for their ability to induce transcription of the cat gene upon low multiplicit ... | 1986 | 3005609 |
| pathogenicity in mice of herpes simplex virus type 2 mutants unable to express glycoprotein c. | herpes simplex virus type 2 (hsv-2) mutants that were unable to express glycoprotein c (gc-2) were isolated. deletions were made in a cloned copy of the gc-2 gene, and recombinant viruses containing these deletions were screened by using an immunoreactive plaque selection protocol. the viruses did not display a syncytial phenotype. intravaginal inoculation of balb/cj mice with one of the hsv-2 gc-2- viruses produced local inflammation followed by a lethal spread of the viral infection into the n ... | 1986 | 3005656 |
| effects of mercury (ii) compounds on the activity of dutpases from various sources. | the deoxyuridine triphosphate nucleotidohydrolases (dutpases, ec 3.6.1.23) from escherichia coli k-12-,acholeplasma laidlawii b-pg9-, human kb cell-, and the herpes simplex virus (hsv) type 1- and 2-induced dutpases were purified and used to determine the effect of various mercury (ii) compounds on their activities. mercuric acetate, 5-mercuri-dutp (hgdutp), and 5-mercuri-dctp (hgdctp) acted as irreversible active site-directed inhibitors of the dutpases purified from eukaryotic organisms but no ... | 1986 | 3005836 |
| infections with herpes simplex viruses (1). | 1986 | 3005858 | |
| dna amplification and neoplastic transformation mediated by a herpes simplex dna fragment containing cell-related sequences. | the transforming potential of the herpes simplex virus type 2 (hsv-2) bamhi fragment e (map position 0.533-0.583) encoding the 140-kda ribonucleotide reductase was assayed by transfection in established rat-2 cells. foci of refractile, morphologically distinguishable cells were induced at lower efficiency and after a longer incubation period as compared to the human tumor oncogene ej-ha-ras. focus-derived bamhi fragment e-transformed cell lines formed medium-to-large (0.1-0.25 mm) colonies in so ... | 1986 | 3006067 |
| intracellular localization and serological identification of a hsv-2 protein in cervical cancer. | exfoliated atypical cells from us and italian patients with cervical intraepithelial neoplasia or invasive cancer stained in indirect immunofluorescence with a monoclonal antibody to a high mw hsv-2 protein (icp10). the proportion of staining cells increased as a function of the severity of the cervical anaplasia. in the majority of cases with mild or moderate dysplasia staining was restricted to the cytoplasm while atypical cells from 6 of 7 cases with marked dysplasia and 7 of 7 cases with inv ... | 1986 | 3009194 |
| genital reinfection after recovery from initial genital infection with herpes simplex virus type 2 in guinea pigs. | the natural history of genital reinfection with herpes simplex virus type 2 (hsv-2) three weeks to one year after initial infection was explored by using a guinea pig model. intravaginal reinoculation with hsv-2 of animals that had recovered from initial genital infection produced by wild-type or thymidine kinase-deficient hsv-2 produced asymptomatic genital reinfection characterized by high-titered replication of hsv-2 in the vaginal vault. reinoculation did not result in acute neural infection ... | 1986 | 3009643 |
| synthesis and antiviral properties of 5-(2-substituted vinyl)-6-aza-2'-deoxyuridines. | the following 5-(2-substituted vinyl)-6-aza-2'-deoxyuridines were synthesized: (e)-5-(2-bromovinyl) (2) (6-aza-bvdu), 5-(2-bromo-2-fluorovinyl) (a mixture of e and z isomers) (3), (e)-5-(2-chlorovinyl) (4), (e)-5-[2-(methylthio)vinyl] (5), 5-(2,2-dibromovinyl) (6), and 5-(3-furyl) (7). the synthesis of 2-6 utilized wittig-type reactions on 5-formyl-1-(2'-deoxy-3', 5'-di-o-p-toluoyl-beta-d-erythro-pentofuranosyl)-6-azauracil (16). 6-aza-bvdu (and its alpha-anomer) was also synthesized from (e)-5- ... | 1986 | 3009815 |
| cloning and characterization of oril2, a large palindromic dna replication origin of herpes simplex virus type 2. | an origin of replication within the long unique sequence of herpes simplex virus type 2 designated oril2 has been identified in a position homologous to its type 1 counterpart, oril1, between map coordinates 0.398 and 0.413. the difficulties encountered in previous attempts to clone both oril2 and oril1 in an undeleted form were surmounted by minimizing the growth of the host escherichia coli, using a recbc sbcb e. coli host, and purifying the full-length plasmid from delected forms by using a n ... | 1986 | 3009865 |
| expression of herpes simplex virus glycoproteins in polarized epithelial cells. | members of the herpesvirus family mature at inner nuclear membranes, although a fraction of the viral glycoproteins is expressed on the cell surface. in this study, we investigated the localization of herpes simplex virus type 2 (hsv-2) glycoproteins in virus-infected epithelial cells by using a panel of monoclonal antibodies directed against each of the major viral glycoproteins. all of the hsv-2 glycoproteins were localized exclusively on the basolateral membranes of vero c1008, madin-darby bo ... | 1986 | 3009881 |
| identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the l component of herpes simplex virus genome. | in the course of studies on the a sequences located at the termini of and at the junction between the l and s components of herpes simplex virus 1 dna, j. chou and b. roizman (j. virol. 57:629-637, 1986) noted that the a sequence acted as a gamma 1 promoter when fused to the structural sequence of the thymidine kinase gene, the b inverted repeat sequences located in the l component next to the a sequences contained an open reading frame predicted to encode the protein of 358 amino acids with a m ... | 1986 | 3009891 |
| effect of delta 9-tetrahydrocannabinol on herpes simplex virus type 2 vaginal infection in the guinea pig. | the present investigation was undertaken to determine whether delta 9-tetrahydrocannabinol (delta 9-thc) decreases host resistance to herpes simplex virus type 2 vaginal infection in the guinea pig. the guinea pig was selected as the host since it has been shown to express a spectrum of primary herpes genitalis which is similar to that in humans. animals were administered delta 9-thc or vehicle intraperitoneally on days 1-4, 8-11, and 15-18. herpes simplex virus was introduced intravaginally on ... | 1986 | 3010327 |
| recurrent genital herpes and suppressive oral acyclovir therapy. relation between clinical outcome and in-vitro drug sensitivity. | to evaluate the association between in-vitro resistance of herpes simplex virus type 2 to acyclovir and breakthrough recurrences of herpes despite chronic suppressive therapy, we determined the in-vitro sensitivity of herpes simplex virus isolated before, during, and after therapy. one hundred eighty-three virus isolates from 107 patients were tested. before therapy, the median amount of drug required to inhibit 50% of the virus in tissue culture (id50) was 0.91 microgram/ml. the median id50 aft ... | 1986 | 3010782 |
| purulent penile ulcers of patients in singapore. | in 80 patients with painful purulent penile ulcers who attended the outpatient service of middle road hospital in singapore, haemophilus ducreyi was isolated from 18 (22%), herpes simplex virus type 2 from nine (11%), and neisseria gonorrhoeae from eight (10%). primary pathogens were not isolated from 45 (57%) men. painful purulent penile ulcers were more common in uncircumcised men, and patients had often acquired the disease after sexual intercourse with prostitutes in singapore. | 1986 | 3015775 |
| prospective study on the relationship between cervical neoplasia and herpes simplex type 2 virus. iii. presence of herpes simplex type-2 antibody in sera of subjects who developed cervical neoplasia later in the study. | paired sera taken from 63 subjects who developed cervical neoplasia in the course of a prospective study on this disease were examined for the presence of herpes simplex virus type 2 (hsv-2) antibody. the first serum was taken at enrollment into the study, while the second was obtained after development of the disease, i.e. 2-4 years later. simultaneously, paired sera from a group of control subjects, who remained free of any cytological and colposcopical abnormalities throughout the study, were ... | 1986 | 3015805 |
| antibody response, recurrence patterns and subsequent herpes simplex virus type 2 (hsv-2) re-infection following initial hsv-2 infection of guinea-pigs: effects of acyclovir. | the production of antibody to specific herpes simplex virus type 2 (hsv-2) polypeptides, the recurrence patterns and the susceptibility to re-infection were studied in the guinea-pig model of genital hsv-2 infection. further, we defined the effects of acyclovir (acv) therapy on these parameters of infection. treatment with acv reduced the clinical severity of the initial disease but did not affect vaginal viral shedding. production of neutralizing antibody as well as antibody to the nucleocapsid ... | 1986 | 3016155 |
| asymptomatic virus shedding in men with genital herpes infection. | 13 men with a history of recurrent genital herpes simplex virus type 2 (hsv-2) infection were followed daily for 4 weeks with samples taken from the urethra for virus isolation. asymptomatic virus shedding occurred in 5 men who had 1 single positive isolation each. four of these urethral isolates were typed as hsv-1 and 1 as hsv-2. | 1986 | 3016883 |
| a rapid detection of herpes simplex virus by elisa in asymptomatic pregnant women and neonates. | herpes simplex virus (hsv) was detected by the enzyme-linked immunosorbent assay (elisa). the assay system employed rabbit anti-hsv-2-coated microplates to detect hsv in clinical specimens and the same reagent labelled with peroxidase as a conjugate. the hsv type 2 obtained from vero cell culture and normal cell lysate (ncl) were used as positive and negative reference antigens respectively. hsv was detected in 40 (9.93%) of vaginal swabs obtained from 403 pregnant women just before the deliveri ... | 1986 | 3016912 |
| the herpes simplex virus type 2 equivalent of the herpes simplex virus type 1 us7 gene and its flanking sequences. | nucleotide sequencing studies (d. j. mcgeoch, a. dolan, s. donald, and f. rixon, 1985, j. mol. biol. 181, 1-14) have indicated that herpes simplex virus type 1 (hsv-1) has a coding sequence, referred to as us7, between the genes for the glycoproteins d and e (gd and ge). northern blot analysis and nucleotide sequencing have been carried out to show that the type 2 virus (hsv-2) has an equivalent to the us7 gene. a comparison with the hsv-1 sequence has revealed some surprising similarities and d ... | 1986 | 3016980 |
| delta 9-tetrahydrocannabinol decreases alpha/beta interferon response to herpes simplex virus type 2 in the b6c3f1 mouse. | this study was undertaken to determine the effect of delta 9-tetrahydrocannabinol (delta 9-thc) on polyinosinic:polycytidylic acid [poly(i):poly(c)]-induced, and on herpes simplex virus type 2 (hsv-2)-induced, alpha/beta interferon in the b6c3f1 mouse. animals were administered delta 9-thc, or the diluent, intraperitoneally for 4 consecutive days or at various time intervals prior to administration of the interferon inducer. poly(i):poly(c) or hsv-2 was injected intravenously on day 4. animals r ... | 1986 | 3003756 |
| antiviral activity of 5'-paa and 5'-pfa phosphate esters of 2'-deoxyuridines. | the 5'-paa and 5'-pfa phosphate esters of 5-bromo-2'-deoxyuridine (budr) were synthesized and their antiherpes virus activity was evaluated in vitro. both compounds showed activity of the same order as the parent nucleoside, budr, against hsv-2 but were 4 to 12 times less potent against hsv-1. the 5'-paa phosphate ester of budr (ro 21-9875) was also active against varicella-zoster virus (vzv) and human cytomegalovirus (hcmv). the 5'-paa phosphate ester of 5-bromovinyl-2'-deoxyuridine (bvdu) was ... | 1986 | 2947889 |
| evidence that neomycin inhibits hsv 1 infection of bhk cells. | the effect of neomycin on the herpes simplex virus (hsv) type 1 and 2 infection of baby hamster kidney cells was studied. neomycin concentrations of 3 mm or more caused a more than 90% inhibition of hsv 1 proliferation, while it had no effect on hsv 2 proliferation, measured as plaque-forming units. furthermore, neomycin must be present at the time of infection in order to exert full effect, addition 1 hour postinfection was comparable to untreated cells. this indicates that neomycin may specifi ... | 1986 | 3026374 |
| [immunohistological study on malignant and premalignant lesions of the uterine cervix associated with herpes simplex virus]. | herpes simplex virus 2 (hsv-2) has become the object of public attention as an etiological cause of cervical cancer. uneven distribution of hsv antigen in tissue was examined dy dyeing tissue materials of cervical intraepithelial neoplasia by the method called peroxidase-antiperoxidase using hsv-2 antibody. the positive rate in the control group was 10.3%, while it was 10.7% in the mild dysplasia group, 17.5% in the moderate dysplasia group, 25.5% in the severe dysplasia group, 31.3% in the carc ... | 1986 | 3025314 |
| isolation and characterization of expressible cdna clones encoding the m1 and m2 subunits of mouse ribonucleotide reductase. | mammalian ribonucleotide reductase consists of two nonidentical subunits, proteins m1 and m2, which are differentially regulated during the cell cycle. we have isolated expressible cdna clones of both subunits from an okayama-berg cdna library made with mrna from hydroxyurea-resistant, m2 protein-overproducing mouse ta3 cells. expression of m2 protein could be demonstrated by electron paramagnetic resonance spectroscopy after transfection of cos-7 monkey cells with the plasmid. electrophoresis a ... | 1986 | 3025593 |
| efficacy of vaccination with skinner vaccine towards the prevention of herpes simplex virus induced cervical carcinoma in an experimental mouse model. | the incidence of cervical carcinoma, which had been induced by vaginal application of inactivated herpes simplex virus type 2 (hsv-2) with 20% croton oil, was significantly reduced in mice prevaccinated with the skinner herpes vaccine. there was evidence of an immunological response in both vaccinated and unvaccinated mice. | 1986 | 3026107 |
| herpes simplex type ii encephalitis in infancy presenting with focal encephalitis. | herpes virus was recovered from a throat swab, nasopharyngeal washings as well as from brain tissue from a six-month-old infant, who presented with fever, left focal seizures and an enhancing right frontal ct-scan lesion. cytopathic effect (cpe) as seen with genital herpetic infection was seen, suggesting hsv-2. immunofluorescent typing of the virus isolate confirmed hsv-2. early igm positivity preceding a cf and sn titer rise was observed. the patient received a course of ara-a and recovered wi ... | 1986 | 3027605 |
| prevalence of htlv iii/lav and other viral antibodies in normal and at risk populations. | the prevalence of htlv iii/lav antibody has been studied in six groups of population: healthy subjects, a virological laboratory staff, prisoners, drug addicts, patients with lymphoadenopathy-associated syndrome (las) and patients with aids. prevalences and levels in the same groups of hbsag and cmv, hsv 1 and hsv 2 antibodies have been studied, too. as expected, the prevalence of htlv iii/lav antibody increases in the six groups with the increasing of the risk while the prevalences of the other ... | 1986 | 3030363 |
| viral alkaline nuclease in intranuclear dense bodies induced by herpes simplex infection. | the distribution of the herpes simplex virus alkaline nuclease (hsv dnase) in rabbit fibroblast cells infected with hsv type 1 or 2 (hsv-1 or hsv-2) has been examined with the aid of immunocytochemical techniques using gold particles as markers. hsv dnase was found to be accumulated within infected nuclei as early as 2.5 hr post-infection. labeled antibody to hsv dnase subsequently increased in all nuclei after 7 hr and 17 hr. at all times post-infection the virus induced nuclear dense bodies we ... | 1986 | 3032315 |
| herpes simplex virus gene products involved in the induction of chromosomal aberrations. | the effect of short-term herpes simplex virus type 1 (hsv-1) infection on chromosomes of human diploid fibroblasts was examined. in addition to chromosomal breaks, gaps and pulverization, three kinds of cytogenetic damage (double minutes, polyploidy and endoreduplication) not yet reported following productive infection with hsv or other animal viruses were frequently observed. consistent with previous studies suggesting that the expression of immediate-early and/or early viral gene products is r ... | 1986 | 3033932 |
| incidence of herpes genitalis in northern ireland in 1973-83 and herpes simplex types 1 and 2 isolated in 1982-4. | during 1973 - 83 there was a pronounced rise in the number of new cases of herpes genitalis in northern ireland. this study of 127 patients with herpes genitalis shows that 30% had herpes simplex virus type 1 (hsv 1) and 70% had herpes simplex virus type 2 (hsv 2) infections. of 41 sexual contacts who attended, only seven had herpes genitalis (caused in each case by hsv 2); another patient had a history of genital lesions, and one other of oral lesions, but hsv was not isolated from either patie ... | 1986 | 3949348 |
| congenital herpes simplex retinitis. | an 1,810-g girl born at 37 weeks of gestation had true congenital herpes simplex virus type 2 infection. the neonate was born with hypo-pigmented skin lesions, brain lesions, and old heavily pigmented retinal scars in the posterior pole. there was no active ocular disease. viral cultures, immunoperoxidase stains, and antibody determination by enzyme-linked immunosorbent assay were all positive for herpes simplex virus. treatment with acyclovir healed the lesions within ten days. the clinical and ... | 1986 | 3728622 |
| role of virus-infected mononuclear leukocytes in herpetic chorioretinitis of newborn rabbits. | the role of virus-infected mononuclear leukocytes (mnls) in the pathogenesis of neonatal herpetic chorioretinitis in newborn rabbits was investigated. as early as 2 days after inoculating the animals' skins with type 2 herpes simplex virus (hsv-2), infectious mnls in the infected animals' peripheral blood were found. the virus was associated, for the most part, with mnls that belonged to phagocytic and adherent cell fractions. observations by electron microscopy indicated that hsv-2 was actively ... | 1986 | 3759364 |
| delayed treatment with combinations of antiviral drugs in mice infected with herpes simplex virus and application of the median effect method of analysis. | mice were inoculated intracerebrally with a lethal dose of herpes simplex virus type 2. three days later, the mice were treated intraperitoneally, twice daily for 4 days, with the following drugs alone or in combination: acyclovir (acv), vidarabine (ara-a), 2'-fluoro-5-iodoaracytosine (fiac), and 2'-fluoro-5-methylarauracil (fmau). despite delayed treatment, most of the animals receiving low doses of fmau alone or in combination with acv or ara-a survived. in contrast, significantly higher morta ... | 1986 | 3777913 |
| xylotubercidin against herpes simplex virus type 2 in mice. | of a series of newly synthesized derivatives of the pyrrolo[2,3-d]pyrimidine nucleosides tubercidin, toyocamycin, and sangivamycin, the xylosyl analog of tubercidin, xylotubercidin, exhibited the greatest potency and selectivity against herpes simplex virus type 2 (hsv-2) in vitro. at dosage regimens that were not toxic for the host, xylotubercidin proved efficacious in various hsv-2 animal model infections. when applied topically at 0.25, 0.5, or 1% in dimethyl sulfoxide or when administered sy ... | 1986 | 3800347 |
| oral bromovinyldeoxyuridine therapy for herpes simplex and varicella-zoster virus infections in severely immunosuppressed patients: a preliminary clinical trial. | twenty-five patients with haematological diseases were treated orally with the highly potent and selective anti-herpes agent, bromovinyldeoxyuridine (bvdu), in a dosage of 7.5 mg/kg/day (divided over three or four doses a day) for 5 days for an intercurrent mucocutaneous herpesvirus infection. of these 25 patients, 8 were severely granulocytopenic at the time of the viral infection, and 12 recently had undergone bone-marrow transplantation; 5 were under cytotoxic therapy for a lymphoproliferativ ... | 1986 | 3511181 |
| a placebo-controlled trial of topical 8% arildone cream early in recurrent genital herpes. | arildone is an aryl diketone which is inhibitory in vitro against herpes simplex virus type 2 at a concentration of 2 micrograms/ml or less. one hundred forty-five patients with recurrent genital herpes were enrolled in a multicenter, randomized, placebo-controlled, double-blind trial to evaluate the efficacy and safety of an 8% arildone cream. patients initiated therapy a mean of 9.9 h and a maximum of 24 h after the reported onset of lesions and applied medication 6 times daily for 7 days. the ... | 1986 | 3521487 |
| [group b streptococcal panophthalmia in neonatal infection]. | hemolyzing streptococci of group b were isolated from an ulcerative keratitis in a 14-day-old girl (gestational age: 39 weeks, birth weight: 3230 g) suffering from septicemia. the differential diagnosis includes all bacteria and virus species able to penetrate the intact cornea, e.g. pseudomonas sp., n. gonorrhoeae and herpes simplex virus ii. perinatal infection may be via the birth canal. | 1986 | 3537493 |
| oral cancer induced in hamsters with herpes simplex infection and simulated snuff dipping. | a number of epidemiologic studies indicate that snuff dipping is associated with an increased incidence of oral cancer in human beings. since inactivated herpes simplex virus (hsv) has been shown to induce malignant changes in vitro and in vivo and is partially inactivated by snuff water extract, we examined the histopathologic changes of hamster buccal pouches after exposure to repeated hsv inoculation combined with long-term simulated snuff dipping. one hundred twenty-five syrian hamsters were ... | 1986 | 3462612 |
| effect of antibody to interferon on genital herpesvirus infection in mice. | the effect of anti-mouse interferon (ifn) antibody on the course of genital herpes infection was studied in c57b1/6j mice. as we have previously described, intravaginal inoculation of c57 mice with herpes simplex virus type 2 (hsv-2) results in the development of genital lesions, subsequent encephalitis and death, with the mortality rate dependent on virus dose. in the present study, we found that intravaginal application of 1 x 10(5) units of anti-mouse (alpha/beta) ifn antibody did not signifi ... | 1986 | 3508484 |
| central nervous system and genital infection with reactivation of latent herpes simplex virus type 2 in mice. | to establish a reactivation model of genital and central nervous system infection, 3- to 12-week-old outbred or balb/c mice were inoculated vaginally with the hg-52 strain of herpes simplex virus type 2 (hsv-2). primary infection was confirmed by serially positive vaginal cultures. mortality in 6- and 12-week old infected mice was about 20%. in survivors, clearance of infectious virus was confirmed in serially negative vaginal cultures. at 6 weeks, immunosuppression of survivors with cyclophosph ... | 1986 | 3508488 |
| a comparison of imaging, clinical, and pathologic aspects of space-occupying lesions within the brain in patients with acquired immune deficiency syndrome. | patients with acquired immune deficiency syndrome (aids) often have central nervous system mass lesions. since clinical signs, symptoms, and results of imaging techniques are generally nonspecific, brain biopsy has been considered to be the optimal method for establishing a definitive diagnosis. in an effort to define better the imaging characteristics of such lesions, we retrospectively analyzed 24 patients with aids who had intracerebral space-occupying lesions, with subsequent definitive hist ... | 1986 | 3451758 |
| synthesis, structure, and biological activity of certain 2-deoxy-beta-d-ribo-hexopyranosyl nucleosides and nucleotides. | 2-deoxy-beta-d-ribo-hexopyranosyl nucleosides with adenine (2), hypoxanthine (17), guanine (23), cytosine (13), and uracil (7) as the aglycon were synthesized by the lewis-acid catalyzed condensation of an appropriate trimethylsilylated heterocyclic base and 2-deoxy-1,3,4,6-tetrakis-o-(4-nitrobenzoyl)-beta-d-ribo-hexopyranose+ ++ (5) to provide the desired beta anomers in good yield. when the synthesis of 7 via an sn2 displacement was attempted by reaction between silylated uracil and 2-deoxy-3, ... | 1987 | 3585903 |
| detection of asymptomatic herpes simplex virus infections after vaccination. | twenty-two volunteers seronegative for antibodies to herpes simplex virus (hsv) were enrolled in a trial to determine tolerance and immunogenicity of an hsv-2 glycoprotein subunit vaccine. vaccine was administered at days 0, 28, and 140, and sera were obtained on days 0, 7, 14, 21, 28, 35, 49, 56, 140, 147, and 365 for determination of hsv neutralizing antibody activity and antibody-dependent cell cytotoxicity (adcc). sera were also tested by immunoprecipitation of radiolabeled hsv-2-infected ce ... | 1987 | 3806788 |
| intrauterine herpes simplex virus infections. | neonatal herpes simplex virus (hsv) infection is usually acquired at birth, although a few infants have had findings suggestive of intrauterine infection. we describe 13 babies who had clinical manifestations of intrauterine hsv infection, including skin lesions and scars at birth (12), chorioretinitis (eight), microcephaly (seven), hydranencephaly (five), and microphthalmia (two). all infants had combinations of these defects. infection was proved by viral isolation in each case; all isolates w ... | 1987 | 3794894 |
| production, binding characteristics, and immunogenicity of heterologous anti-idiotypic antibody to herpes simplex virus glycoprotein c. | a monoclonal antibody specific for glycoprotein c (gc) of herpes simplex virus type 1 (hsv-1) was used to prepare a heterologous anti-idiotypic antibody in rabbits. after absorption with normal mouse immunoglobulin (nms) the anti-idiotypic (anti-id) antibody retained binding activity for moab d4.1, the immunogen. the anti-id (anti-id c) also demonstrated a cross-reactive binding activity, as shown by elisa, for moab d4.2 and moab d4.8 which was specific for glycoprotein d (gd) and glycoprotein b ... | 1987 | 3509672 |
| relationship of smoking to lifestyle factors in women. | the purposes of this research were to examine the relationship of female smoking and lifestyle factors and to assess the applicability of an index of "emancipation" to smoking status in women who live in the province of ontario. the index of "emancipation," developed by eide, was based on distinguishing women who are not following traditional roles as housewives from those who are. while reports in the literature confirm that occupational status, marital status and educational achievement are re ... | 1987 | 3424848 |
| the cellular changes in primary and recurrent infection with herpes simplex virus type 2 in an in vitro model. | the cellular changes of primary and recurrent herpes simplex virus (hsv) infection were investigated in in vitro models. in the primary infection model, nuclear changes were characterized by clumping and margination of the nuclear chromatin, a homogeneous ground-glass nuclear appearance, multinucleation and the appearance of two different types of intranuclear inclusions. one of the two types of inclusions appeared as early as six hours postinfection, reached a maximum at nine hours postinfectio ... | 1987 | 3425155 |
| antiviral activity of phosphonylmethoxyalkyl derivatives of purine and pyrimidines. | various 3-hydroxy-2-phosphonylmethoxypropyl (hpmp) and 2-phosphonylmethoxyethyl (pme) derivatives of purine [adenine (a), guanine (g), 2,6-diaminopurine (dap), 2-monoaminopurine (map), hypoxanthine (hx)] and pyrimidine [cytosine (c), uracil (u), thymine (t)] have been evaluated for their antiviral properties. pmedap, (s)-hpmpa [and the cyclic phosphonate thereof, (s)-chpmpa)], (s)-hpmpc, pmeg, pmea, hpmpg and hpmpdap proved to be effective inhibitors of herpes simplex virus type 1 (hsv-1) and ty ... | 1987 | 3451698 |
| multiple oral and labial ulcers in an immunocompromised patient. | herpes simplex virus infections in immunocompromised individuals have clinical features that are often quite different from primary and secondary hsv infections in otherwise healthy people. these infections should be recognized early and treated promptly to prevent progression of the lesions and the possible systemic dissemination of the virus. cytological and biopsy examination frequently discloses changes characteristic of hsv-1, hsv-2, or varicella-zoster virus. to ensure the most precise dia ... | 1987 | 3469271 |
| suppression of tumor growth and enhancement of immune status with high levels of dietary vitamin b6 in balb/c mice. | effects of dietary vitamin b6 at levels ranging from deficiency to megadoses on the development of herpes simplex virus type 2-transformed (h238) cell-induced tumors and on in vitro responses relating to cell-mediated immunity were examined. male balb/cbyj mice (n = 260), 5 weeks of age, were fed 20% casein diets containing pyridoxine (pn) at 0.2, 1.2 for the control diet, 7.7, or 74.3 mg/kg diet for 4-11 weeks. after 4 weeks of dietary treatment, 120 of the mice received an injection of h238 ce ... | 1987 | 3472003 |
| vaccinia virus and the egf receptor: a portal for infectivity? | we previously demonstrated that occupancy of the epidermal growth factor (egf) receptor reduced the ability of vaccinia virus to infect l cells [eppstein et al: nature 318:663, 1985]. this result suggested that vaccinia virus was utilizing the egf receptor as one pathway to infect cells. we have studied this system further, and now find that antibodies to the egf receptor also reduce the ability of vaccinia virus to infect cells productively. inclusion of both egf and antibodies to the egf recep ... | 1987 | 3497935 |
| detection of dna viruses by radioactive and non radioactive dna probes: application to african swine fever virus. | a molecular hybridization technique using radioactive and non radioactive dna probes, has been used to detect asfv dna immobilized on nitrocellulose paper. it is based on the use of plasmid prpel-2 as a hybridization probe. this plasmid contain the h-clai dna fragment (size 5.6 kbp) from the spain-70 strain of asfv. the sensitivity of detection using radioactive 32p-probes (specific activity about 2 x 10(8) cpm per microgram) was about 20 pg of viral dna. the 32p-prpel-2 dna probe can detect abo ... | 1987 | 3101653 |
| disseminated neonatal herpes simplex virus infection with necrotizing encephalitis--an autopsy case. | an autopsy case of disseminated hsv type 2 infection occurring in a neonate at 32 weeks' gestation, delivered by cesarean section after premature rupture of membrane of 7 days duration, is presented. herpes simplex virus type 2 was isolated from the vesicular skin lesion. the mother and patient had specific antibody to type 2 herpes simplex virus. patient's parents had denied any herpetic orolabial or genital lesion during or before this pregnancy. cultures from the cervical and vaginal swabs of ... | 1987 | 3271053 |
| herpes simplex virus type-2 encephalitis in peripartum period preceded by hepatitis. | encephalitis by herpes simplex virus type-2 in adults is rare and has been described as part of a disseminated infection in settings of immunosuppression, pregnancy being one of them. the virus was isolated from csf of a young female, who during puerperium, presented with hepatitis, encephalitis and subsequently developed persistent vegetative state. case history of another woman at term pregnancy is described who had similar illness but virological proof could not be obtained. | 1987 | 3573412 |
| the in-vitro and in-vivo efficacy of cisplatin and analogues in the treatment of herpes simplex virus-ii infections. | the antitumour effect of cisplatin results from cross-linking and disruption of dna when it binds to dna bases, especially cytosine and guanine. since herpes simplex virus (hsv) has a high cytosine and guanine content, cisplatin might be expected to have an antiviral effect against hsv. the 50% inhibitory concentration of cisplatin for hsv-ii was 0.06 mg/l. six of ten platinum analogues had 50% inhibition of plaques at less than or equal to 10 mg/l. we evaluated the in-vivo activity of cisplatin ... | 1987 | 3610908 |
| antiherpesvirus activity of 9-(4-hydroxy-3-hydroxy-methylbut-1-yl)guanine (brl 39123) in cell culture. | the activity of 9-(4-hydroxy-3-hydroxymethylbut-1-yl)guanine (brl 39123) against several herpesviruses was compared with that of acyclovir (acv). in plaque reduction tests with clinical isolates of herpes simplex virus type 1 (hsv-1), herpes simplex virus type 2 (hsv-2), and varicella-zoster virus, mean 50% inhibitory concentrations (ic50s) (n = number tested) for brl 39123 were 0.4 (n = 17), 1.5 (n = 13), and 3.1 (n = 5) micrograms/ml, respectively. corresponding ic50s for acv were 0.2, 0.6, an ... | 1987 | 3631945 |
| acute ascending necrotizing myelopathy caused by herpes simplex virus type 2. | a 57-year-old diabetic man died of a rapidly ascending necrotizing myelitis. autopsy results proved that the etiologic agent was herpes simplex virus type 2 (hsv2). the clinical findings, autopsy immunohistopathology, and electron-microscopy suggest that either primary hsv2 infection or reactivation of hsv2 infection within dorsal root ganglia was followed by spread to the spinal cord. viral infection of the cns occurred by direct extension and led to death by involvement of the brainstem. altho ... | 1987 | 3670617 |
| augmentation of natural immune defence mechanisms and therapeutic potential of a mismatched double-stranded polynucleotide in cutaneous herpes simplex virus type 2 infection. | we studied the effect of an analogue of polyinosinic acid:polycytidylic acid, the mismatched poly(ri).poly(rc12u), on herpes simplex virus type 2 (hsv-2)-induced cutaneous disease in the guinea-pig. recurrence patterns and hsv-2-induced immune responses were also defined. intranasal administration (1.5 micrograms/g body weight, five doses at 48 h intervals) of poly(ri).poly(rc12u) during initial hsv-2 infection caused a significant (p less than 0.05) reduction in virus titres in the skin and dec ... | 1987 | 3681265 |
| expression of immunity to intravaginal herpes simplex virus type 2 infection in the genital tract and associated lymph nodes. | herpes simplex virus type 2 is a human venereal pathogen which causes lethal neurological illness after intravaginal inoculation into balb/cj mice. in the present studies, we demonstrate that intravaginal vaccination with an attenuated strain of this virus, which possesses a partial deletion of the thymidine kinase gene, rapidly induced durable immunity to lethal intravaginal challenge with wild-type virus. such immunity was characterized by a dramatic hyperplasia of genital lymph nodes and a si ... | 1987 | 3813891 |
| histopathologic characteristics of two forms of experimental herpes simplex virus retinitis. | herpes simplex virus type 1 (hsv-1) inoculated intracamerally into one anterior chamber of a balb/c mouse produces retinitis in the uninoculated contralateral eye within 7 to 10 days while the retina of the inoculated eye is spared. in sharp contrast, animals receiving hsv type 2 (hsv-2) by the anterior chamber route develop a dramatic retinitis in the inoculated eye by day 7 postinoculation while the retina of the contralateral eye remains uninvolved. histopathologic examination of retinal dest ... | 1987 | 3829704 |
| effect of neuraminidase on fc and c3b receptors on rabbit corneal cells infected with herpes simplex virus. | the effect of neuraminidase on fc receptors (fcr) and c3b receptors (c3br) was studied in epithelial, stromal and endothelial cells of the rabbit cornea infected with type 1 (hsv-1) and type 2 herpes simplex virus (hsv-2) in vitro. fcr were induced on epithelial, stromal and endothelial cells of the rabbit cornea by both hsv-1 and hsv-2, but their activities were not enhanced by neuraminidase. on the other hand, the treatment of hsv-infected corneal cells with neuraminidase resulted in the enhan ... | 1987 | 3829705 |
| virus spread and initial pathological changes in the nervous system in genital herpes simplex virus type 2 infection in mice. a correlative immunohistochemical, light and electron microscopic study. | mice were infected by the vaginal route with the ms strain of herpes simplex virus type 2 (hsv-2). serial vaginal cultures were used to confirm infection and to select mice for this study. two mice were killed by perfusion on days 2-6 post infection (p.i.) and lumbar and sacral cord with cauda were fixed and embedded for electron microscopy. semithin epon-sections were stained for viral antigen using a rabbit anti-hsv-2 antiserum and the avidin-biotin (abc) method. thin sections from antigen-pos ... | 1987 | 3033978 |
| demonstration of viral thymidine kinase inhibitor and its effect on deoxynucleotide metabolism in cells infected with herpes simplex virus. | the thymidine analog 5'-ethynylthymidine was a potent inhibitor of herpes simplex virus type 1 (strain kos)-induced thymidine kinase with a ki value of 0.09 microm. 5'-ethynylthymidine was less inhibitory against herpes simplex virus type 2 (strain 333)-induced thymidine kinase with a ki of 0.38 microm and showed no inhibition against human cytosolic thymidine kinase under the conditions tested. the compound was effective against the altered thymidine kinase induced by acyclovir- and bromovinyld ... | 1987 | 3034143 |
| expression of the hsv specified major dna-binding protein in virus infected raji and vero cells. | we studied the expression of the herpes simplex virus type-2 (hsv-2) specified major dna-binding protein (icsp 11/12) in virus infected raji and vero cells. immunofluorescence staining by a specific icsp 11/12 antiserum distinguished between different cell-virus interactions. icsp 11/12 synthesized in the hsv-2 infected vero cells showed dense homogenous nuclear staining early in the infection. during virus replication the pattern changed into a less dense, somewhat marginating staining. the cor ... | 1987 | 3034205 |
| rapid diagnosis of herpes simplex virus infections in conventional and shell vial cell cultures using monoclonal antibodies. | one hundred specimens for herpes simplex virus (hsv) isolation were tested in parallel by conventional and by centrifugation-enhanced cell culture, followed by identification using monoclonal antibodies to hsv-1 and hsv-2. sensitivity was comparable by the two methods; conventional culture was only marginally slower and was easier to fit into the routine of a busy laboratory. it is, therefore, advocated for hsv detection in clinical specimens. | 1987 | 3034946 |
| effect of micromolar concentrations of delta-9-tetrahydrocannabinol on herpes simplex virus type 2 replication in vitro. | the effect of micromolar concentrations of delta-9-tetrahydrocannabinol (delta-9-thc) on the in vitro replication and biosynthesis of herpes simplex virus type 2 (hsv2) was determined. a 100-fold increase in extracellular virus was recorded for infected vero cells pretreated with 10(-6) m or 10(-5) m drug when compared to infected vehicle-treated controls. however, no significant differences were observed in the production of total infectious virus for any of the vehicle or drug-treated cultures ... | 1987 | 3035199 |
| regulation by recombinant interleukin-2 of protective immunity against recurrent herpes simplex virus type 2 genital infection in guinea pigs. | the goal of our study was to determine whether recombinant interleukin-2 (ril-2) could modify the recurrence pattern of chronic herpes simplex virus type 2 (hsv-2) genital infection in guinea pigs. animals that developed symptomatic acute hsv-2 infection were distributed at 14 days after viral inoculation into several treatment groups, which were similar with respect to the severity of acute disease. three ril-2 dosages administered for 4 weeks in daily subcutaneous injections were tested in thi ... | 1987 | 3035213 |
| identification of a novel herpes simplex virus type 1-induced glycoprotein which complexes with ge and binds immunoglobulin. | we detected a glycoprotein on the surface of cells infected with herpes simplex virus type 1 (hsv-1) which, in conjunction with ge, binds immunoglobulin g (igg). the novel glycoprotein, which has an apparent molecular mass of 70 kilodaltons and was provisionally named g70, was first detected in extracts of hsv-1-infected cells labeled by lactoperoxidase-catalyzed iodination and precipitated with rabbit sera or igg and protein a-sepharose. in subsequent experiments, g70 and ge were coprecipitated ... | 1987 | 3035221 |
| herpes simplex virus type 2 glycoprotein biogenesis: effect of monensin on glycoprotein maturation, intracellular transport and virus infectivity. | the ionophore monensin inhibited the formation of herpes simplex virus type 2 (hsv-2) particles by about 30% but the yields of infectious particles were reduced to 5% and 1% for cell-associated and extracellular virus, respectively. the presence of monensin did not affect the processing of the two viral glycoproteins gb-2 and gg-2. however, two other glycoproteins, gc-2 and gd-2, were not processed to their fully mature forms in the monensin-treated cells and only the faster moving pgc-2 and pgd ... | 1987 | 3037016 |
| virus-specific igm and igg antibody production by b cells during herpes simplex virus type 2-induced immunosuppression as analysed by an immunospot assay. | the mechanism of herpes simplex virus (hsv)-2-induced immunosuppression was analysed by determination of the number of igm and igg antibody-secreting b cells in female balb/c mice using an immunospot assay. primary hsv-1 or -2 as well as homologous or heterologous booster infections at different times were performed. in accordance with earlier results on humoral antibody generation, in contrast to hsv-1, hsv-2 induced only very low numbers of antibody-producing b cells in dose-response experimen ... | 1987 | 3037017 |
| recovery of herpes simplex virus from the corneas of experimentally infected rabbits. | rabbits were inoculated in the left cornea with one of three strains of herpes simplex virus (hsv) i.e. hsv-1 strain 17, hsv-1 strain mckrae, hsv-2 strain hg52, or with and hsv-1 mckrae/hsv-2 hg52 recombinant r40/2. fifty-nine to 67 days after inoculation trigeminal ganglia and corneas were explanted and screened for release of infectious virus. virus was isolated from all left trigeminal ganglia after organ culture irrespective of viral strain. virus was isolated from three of 16 corneas of ani ... | 1987 | 3037020 |
| 2'-fluorinated arabinonucleosides of 5-(2-haloalkyl)uracil: synthesis and antiviral activity. | the synthesis of 5-(2-fluoroethyl)-2'-deoxyuridine (fedu, 4b), its 2'-fluoro analogue 1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-5-(2-fluoroethyl)-1h,3h- pyrimidine-2,4-dione (fefau, 4k), and the 2'-fluoro analogue of the potent antiherpes virus compound 5-(2-chloroethyl)-2'-deoxyuridine (cedu), 5-(2-chloroethyl)-1-(2-deoxy-2-fluoro-beta-d-arabinofuranosyl)-1h,3h-pyr imidine - 2,4-dione (cefau, 4i), is described. the antiviral activities of these compounds were determined in cell culture again ... | 1987 | 3037079 |
| the 65,000-mr dna-binding and virion trans-inducing proteins of herpes simplex virus type 1. | the possible identity of the herpes simplex virus type 1 (hsv-1) 65k (65,000-mr) virion protein which stimulates transcription from immediate-early genes with the hsv-1 65k dna-binding protein was investigated. the two proteins were found to be distinct by the three separate criteria of immunological reactivity, tryptic peptide fingerprinting, and mobility in two-dimensional gels. using hsv-1/hsv-2 intertypic recombinants and a serotype-specific antiserum, we located the gene encoding the 65k dn ... | 1987 | 3037105 |
| cells expressing herpes simplex virus glycoprotein gc but not gb, gd, or ge are recognized by murine virus-specific cytotoxic t lymphocytes. | to determine which viral molecule(s) is recognized by herpes simplex virus (hsv)-specific cytotoxic t lymphocytes (ctl), target cells were constructed which express individual hsv glycoproteins. a mouse l cell line, z4/6, which constitutively expressed high levels of hsv type 2 (hsv-2) gd (gd-2) was isolated and characterized previously (d. c. johnson and j. r. smiley, j. virol. 54:682-689, 1985). despite the expression of gd on the surface of z4/6 cells, these cells were not killed by anti-hsv- ... | 1987 | 3037106 |
| sensitivity in vitro of herpes simplex virus isolates to human fibroblast interferon. | sensitivity of herpes simplex virus (hsv) isolates, from patients with recurrent infections, to human fibroblast beta interferon (ifn), was tested in vitro. among 25 hsv strains, 12 were hsv-1, isolated from facial and labial lesions, and 13 were hsv-2 isolated from genital lesions. viral sensitivity to ifn was examined on epithelial mb cell line by yield reduction and plaque reduction and close correspondence between the two methods was observed. most of hsv-1 isolates were in the same range of ... | 1987 | 3037288 |