Publications

specific and differential activation of mitogen-activated protein kinase cascades by unfamiliar taste in the insular cortex of the behaving rat.	rats were given to drink an unfamiliar taste solution under conditions that result in long-term memory of that taste. the insular cortex, which contains the taste cortex, was then removed and assayed for activation of mitogen-activated protein kinase (mapk) cascades by using antibodies to the activated forms of various mapks. extracellular responsive kinase 1-2 (erk1-2) in the cortical homogenate was significantly activated within <30 min of drinking the taste solution, without alteration in the ...	1998	9822758
cloning and functional expression of rat ether-à-go-go-like k+ channel genes.	1. screening of rat cortex cdna resulted in cloning of two complete and one partial orthologue of the drosophila ether-à-go-go-like k+ channel (elk). 2. northern blot and reverse transcriptase-polymerase chain reaction (rt-pcr) analysis revealed predominant expression of rat elk mrnas in brain. each rat elk mrna showed a distinct, but overlapping expression pattern in different rat brain areas. 3. transient transfection of chinese hamster ovary (cho) cells with rat elk1 or rat elk2 cdna gave ris ...	1998	9824707
electron microscopic studies of antlerogenic cells from five developmental stages during pedicle and early antler formation in red deer (cervus elaphus).	previous studies using light microscopy have revealed that histogenesis of deer pedicle and antler has four ossification stages. the first of these stages is the development of the permanent pedicle. initial development of the pedicle is from the cellular layer cells of the antlerogenic periosteum and these cells have been termed initial antlerogenic cells (iacs). apart from the iacs, it has also been shown that the cellular layer cells of the apical periosteum/perichondrium, the peripheral peri ...	1998	9845209
malignant schwannoma in a red deer (cervus elaphus).	a five-year-old female red deer (cervus elaphus) was in poor condition and severely lame on the left hindleg owing to a 19.4 cm x 15.9 cm mass involving and destroying the distal end (head) of metatarsal bones iii and iv, the proximal sesamoid bones and the first phalanges (iii and iv). the histopathological analysis revealed a spindle cell tumour with frequent palisade arrangement (antoni type a pattern), and with highly anaplastic tumour cells in some areas. structures resembling peripheral ne ...	1998	9854770
seasonality of meal patterns and hormonal correlates in red deer.	two groups of six adult, castrated, male red deer were housed under natural daylength conditions and at ambient temperature at 57 degrees n and fed ad lib. (al) or at a fixed rate of 50 g/kg0.75 initial liveweight per day throughout the study (restricted, r). mean daily intakes of al animals were higher during periods of long daylength than during short daylength (p < 0.001). the higher rates of food intake during periods of long daylength were a function of greater meal durations (p < 0.001), s ...	1998	9855479
protection against infection and abortion induced by virulent challenge exposure after oral vaccination of cattle with brucella abortus strain rb51.	to determine efficacy of orally administered brucella abortus vaccine strain rb51 against virulent b abortus challenge exposure in cattle as a model for vaccination of wild ungulates.	1998	9858409
isolation and characterization of proteoglycans from growing antlers of wapiti (cervus elaphus).	proteoglycans were extracted with 4 m guanidine-hcl from the zone of maturing chondrocytes, the site of endochondral ossification of growing antlers of wapiti (cervus elaphus). proteoglycans were isolated by deae-sephacel chromatography and separated by sepharose cl-4b chromatography into three fractions. fraction i contained a high molecular mass (> 1000 kda) chondroitin sulfate proteoglycan capable of interacting with hyaluronic acid. its amino acid composition resembled that of the cartilage ...	1998	9972315
association releases new pediatric discharge guides.	an expert panel convened by the american academy of pediatrics (aap) in elk grove village, il, says traditional guidelines for the discharge of high-risk newborns are inadequate because they can't be tailored easily to many individual cases. in its own just-released guidelines, the aap distinguishes among four categories of high-risk newborns, including preterm infants, infants who require technological support, those at risk because of family issues, and those whose irreversible condition may r ...	1998	10182863
new phylogenetic perspectives on the cervidae (artiodactyla) are provided by the mitochondrial cytochrome b gene.	the entire mitochondrial cytochrome b (cyt b) gene was compared for 11 species of the artiodactyl family cervidae, representing all living subfamilies, i.e., the antlered cervinae (cervus elaphus, c. nippon, dama dama), muntiacinae (muntiacus reevesi), and odocoileinae (odocoileus hemionus, mazama sp., capreolus capreolus, c. pygargus, rangifer tarandus, alces alces); and the antlerless hydropotinae (hydropotes inermis). phylogenetic analyses using tragulidae, antilocapridae, giraffidae and bovi ...	1998	9628037
grb10 identified as a potential regulator of growth hormone (gh) signaling by cloning of gh receptor target proteins.	the cloning of receptor targets procedure, used so far to identify proteins associated with tyrosine kinase receptors was modified to clone sh2 proteins able to bind to the growth hormone receptor (ghr). the cytoplasmic region of ghr, a member of the cytokine receptor superfamily does not contain tyrosine kinase activity. it was thus phosphorylated in bacteria by the elk tyrosine kinase and radiolabeled to screen a mouse expression library. with this probe, we identified shc and the p85 subunit ...	1998	9632636
statistical confidence for likelihood-based paternity inference in natural populations.	paternity inference using highly polymorphic codominant markers is becoming common in the study of natural populations. however, multiple males are often found to be genetically compatible with each offspring tested, even when the probability of excluding an unrelated male is high. while various methods exist for evaluating the likelihood of paternity of each nonexcluded male, interpreting these likelihoods has hitherto been difficult, and no method takes account of the incomplete sampling and e ...	1998	9633105
studies on the parietal region of the cervid skull. iii. on the occurrence of an interparietal in cervus.	the occurrence of an os interparietal was studied in two transparent preparations of fetal red deer (cervus elaphus) heads and in 14 dried skulls of fetal to early postnatal individuals from four cervus species (c. elaphus, c. nippon, c. duvauceli and c. eldi). in 14 of the 16 specimens, an interparietal was present as either a paired or single bone. in only a neonate red deer and a 5-week-old sika deer this skull element was missing. we therefore conclude that an os interparietal, developing fr ...	1998	9652148
caveolin-mediated regulation of signaling along the p42/44 map kinase cascade in vivo. a role for the caveolin-scaffolding domain.	the p42/44 mitogen-activated protein (map)-kinase cascade is a well-established signal transduction pathway that is initiated at the cell surface and terminates within the nucleus. more specifically, receptor tyrosine kinases can indirectly activate raf, which in turn leads to activation of mek and erk and ultimately phosphorylation of elk, a nuclear transcription factor. recent reports have suggested that some members of p42/44 map kinase cascade can be sequestered within plasmalemmal caveolae ...	1998	9654135
serotonergic repression of mitogen-activated protein kinase control of the calcitonin gene-related peptide enhancer.	we have investigated the mechanisms underlying regulation of the calcitonin gene-related peptide (cgrp) cell-specific enhancer. recently, we reported that this enhancer is inhibited by serotonin type-1 (5-ht1) agonists, similar to currently used antimigraine drugs. we have now tested whether this repression involves a mitogen-activated protein (map) kinase pathway. we first demonstrate that the cgrp enhancer is strongly (10-fold) activated by a constitutively active map kinase kinase (mek1), yie ...	1998	9658404
suppression of c-fos gene transcription with malignant transformation of human bronchial epithelial cells.	the activator protein-1 (ap-1) complex is a dimeric transcription factor composed of fos and jun proteins that regulates cellular growth and differentiation. we previously demonstrated a reduction in basal ap-1 transcriptional activity associated with the malignant transformation of human bronchial epithelial (hbe) cells that was, in part, a consequence of decreased c-fos expression. in this study, we investigated the mechanisms underlying the reduction in c-fos expression associated with the ma ...	1998	9662337
assessment of the roles of mitogen-activated protein kinase, phosphatidylinositol 3-kinase, protein kinase b, and protein kinase c in insulin inhibition of camp-induced phosphoenolpyruvate carboxykinase gene transcription.	transcription of the phosphoenolpyruvate carboxykinase (pepck) gene is induced by glucagon, acting through camp and protein kinase a, and this induction is inhibited by insulin. conflicting reports have suggested that insulin inhibits induction by camp by activating the ras/mitogen-activated protein kinase (mapk) pathway or by activating the phosphatidylinositol 3-kinase (pi3-kinase), but not mapk, pathway. insulin activated pi3-kinase phosphorylates lipids that activate protein kinase b (pkb) a ...	1998	9668048
stress-induced immediate-early gene, egr-1, involves activation of p38/jnk1.	the ras/raf/map kinase (erk) pathway is a major signaling pathway induced by growth factors in mammalian cells. two other types of mammalian map kinases, jnk (sapk) and p38 (rk, csbp), are induced by environmental stress. although the immediate-early gene, egr-1, is induced by growth factors, cytokines, differentiation signals and dna damaging agents, less is known about its induction by environmental stress and the mechanism involved. here we report that in nih3t3 cells, egr-1 is induced by var ...	1998	9671412
cdc42 and fgd1 cause distinct signaling and transforming activities.	activated forms of different rho family members (cdc42, rac1, rhoa, rhob, and rhog) have been shown to transform nih 3t3 cells as well as contribute to ras transformation. rho family guanine nucleotide exchange factors (gefs) (also known as dbl family proteins) that activate cdc42, rac1, and rhoa also demonstrate oncogenic potential. the faciogenital dysplasia gene product, fgd1, is a dbl family member that has recently been shown to function as a cdc42-specific gef. mutations within the fgd1 lo ...	1998	9671479
the src homology 2 and phosphotyrosine binding domains of the shcc adaptor protein function as inhibitors of mitogenic signaling by the epidermal growth factor receptor.	upon ligand activation, the epidermal growth factor receptor (egfr) becomes tyrosine-phosphorylated, thereby recruiting intracellular signaling proteins such as shc. egfr binding of shc proteins results in their tyrosine phosphorylation and subsequent activation of the ras and erk pathways. shc interaction with activated receptor tyrosine kinases is mediated by two distinct phosphotyrosine interaction domains, an nh2-terminal phosphotyrosine binding (ptb) domain and a cooh-terminal src homology ...	1998	9685397
p38 mitogen-activated protein kinase mediates the transcriptional induction of the atrial natriuretic factor gene through a serum response element. a potential role for the transcription factor atf6.	in various cell types certain stresses can stimulate p38 mitogen-activated protein kinase (p38 mapk), leading to the transcriptional activation of genes that contribute to appropriate compensatory responses. in this report the mechanism of p38-activated transcription was studied in cardiac myocytes where this mapk is a key regulator of the cell growth and the cardiac-specific gene induction that occurs in response to potentially stressful stimuli. in the cardiac atrial natriuretic factor (anf) g ...	1998	9685422
binding of ets family members is important for the function of the c-sis/platelet-derived growth factor-b tata neighboring sequence in 12-o-tetradecanoylphorbol-13-acetate-treated k562 cells.	the c-sis/platelet-derived growth factor (pdgf)-b tata neighboring sequence (tns) is a promoter element that is required for the full induction of this gene in k562 erythroleukemia cells undergoing 12-o-tetradecanoylphorbol-13-acetate-mediated megakaryoblastic differentiation. nuclear factors from k562 cells can bind to the c-sis/pdgf-b tns, generating four complexes in electrophoretic mobility shift assays. one of these complexes was previously shown to contain sp family members. in this work, ...	1998	9690620
induction of apoptosis by elk-1 and deltaelk-1 proteins.	elk-1, an ets related gene codes for at least two splice variants elk-1, which regulates c-fos transcription and deltaelk-1, both of which function as transcriptional activators. to investigate the role of elk-1 and deltaelk-1 proteins in apoptosis; we have developed rat fibroblast cell lines and human breast cancer cell lines expressing elk-1 and deltaelk-1. the expression of elk-1 and deltaelk-1 proteins in the elk-1/deltaelk-1 transfectants were analysed by immunofluorescence, immunohistochem ...	1998	9696047
mek inhibition augments raf activity, but has variable effects on mitogenesis, in vascular smooth muscle cells.	proteins comprising the mitogen-activated protein (map) kinase signaling cascade are activated by a variety of growth factors, but the precise role of this series of kinase reactions, especially raf kinase and map kinase kinase (mek), in vascular smooth muscle (vsm) cell mitogenesis is not known. in this study, a specific and selective inhibitor of mek, pd-98059, was used to examine the role of mek in dna synthesis and raf-1 activity in vsm cells stimulated with serum as well as with growth fact ...	1998	9696694
genetic analysis of farmed deer hybrids.	molecular methods which identify species-specific genetic markers are valuable for identification and genetic analysis of hybrid deer. we have used a variety of molecular techniques to compare père david's deer elaphurus davidianus, red deer cervus elaphus scotticus and north american wapiti c. e. manatobensis. in total, these analyses have identifed over 300 markers in which père david's deer sires are distinct from farmed red deer and over 100 markers in which wapiti sires differ from red deer ...	1998	9704532
growth, voluntary food intake and digestion in farmed temperate and tropical deer.	growth and voluntary feed intake (vfi) in grazing temperate farmed deer species are influenced by the feeding value of the forage and the stage of the deer's seasonal cycle. liveweight gain (lwg) of growing red deer was greater when perennial ryegrass (0.80)/white clover (0.20) pasture was grazed at 10 cm than 5 cm surface height, but venison production by one year of age was still low. chicory and red clover were of superior feeding value for deer than perennial ryegrass-based pastures, increas ...	1998	9704535
epidemiology of chronic wasting disease in captive rocky mountain elk.	between june 1986 and may 1997, chronic wasting disease (cwd) was the only natural cause of adult mortality among captive rocky mountain elk (cervus elaphus nelsoni) held at a wildlife research facility near fort collins, colorado (usa). of 23 elk that remained in this herd > 15 mo, four (17%) developed cwd. all affected elk were unrelated females from the founding cohort, captured as neonates and raised in 1986. the index case was diagnosed in 1989; time intervals between subsequent cases range ...	1998	9706562
antibody response of elk immunized with porcine zona pellucida.	immunocontraception using porcine zona pellucida (pzp) vaccines is being explored as a nonlethal method of solving the problems of locally overabundant wildlife populations. this study characterized the immunological response of captive elk (cervus elaphus nelsoni) to pzp challenge using 18 3-yr-old cows and was conducted from 14 september 1994 to 13 december 1995. all animals were given a single pzp inoculation and 1 mo later six of these animals were randomly chosen and received a booster inoc ...	1998	9706563
neurotensin type 1 receptor-mediated activation of krox24, c-fos and elk-1: preventing effect of the neurotensin antagonists sr 48692 and sr 142948.	stimulation of neurotensin (nt) type 1 receptors (nt1-r) in transfected cho cells is followed by the activation of mitogen-activated protein kinases and the expression of the early response gene krox24. by making point mutations and internal deletions in the krox24 promoter, we show that proximal serum responsive elements (sre) are involved in transcriptional activation by nt. in addition, we show that the related early response gene c-fos and the ets protein elk-1 are also induced by nt. the in ...	1998	9710257
cloning of a mammalian elk potassium channel gene and eag mrna distribution in rat sympathetic ganglia.	1. three new members of the eag potassium channel gene family were identified in rat and the complete coding sequence of one of these genes (elk1) was determined by cdna cloning. 2. the elk1 gene, when expressed in xenopus oocytes, encodes a slowly activating and slowly deactivating potassium channel. 3. the elk1 gene is expressed in sympathetic ganglia and is also expressed in sciatic nerve. 4. six of the seven known eag genes were found to be expressed in rat sympathetic ganglia, suggesting an ...	1998	9714851
could asynchrony in activity between the sexes cause intersexual social segregation in ruminants?	in many sexually dimorphic mammal species, the sexes live outside the mating season in separate social groups ('social segregation'). social segregation occurs in a wide range of environmental conditions, but its cause in unknown. i suggest that social segregation is caused by a lower level of activity synchrony between individuals in mixed-sex groups than in single-sex groups, owing to sex differences in activity rhythm. as a consequence, mixed-sex groups are more likely to break up than single ...	1998	9718738
alternative splicing of the prolactin receptor gene generates a 1.7 kb rna transcript that is linked to prolactin function in the red deer testis.	a cdna encoding a putative non-membrane bound prolactin receptor was amplified by rt-pcr from red deer (cervus elaphus) testis. sequence analysis suggests that the testicular cdna is generated by alternative splicing resulting in the deletion of exons 7 and 8, which code for: (a) the final 53 aa of the extracellular domain of the receptor including the fifth conserved cysteine residue and the ws x ws motif, (b) the entire transmembrane domain, (c) the first three cytoplasmic amino acid residues, ...	1998	9723863
bradykinin stimulates the erk-->elk-1-->fos/ap-1 pathway in mesangial cells.	among its diverse biological actions, the vasoactive peptide bradykinin (bk) induces the transcription factor ap-1 and proliferation of mesangial cells (s. s. el-dahr, s. dipp, i. v. yosipiv, and w. h. baricos. kidney int. 50: 1850-1855, 1996). in the present study, we examined the role of protein tyrosine phosphorylation and the mitogen-activated protein kinases, erk1/2,in mediating bk-induced ap-1 and dna replication in cultured rat mesangial cells. bk (10(-9) to 10(-7) m) stimulated a rapid i ...	1998	9729506
a diet supplement for captive wild ruminants.	nutritional husbandry of captive wild ruminants often requires feeding these animals a supplemental diet to enhance their health, reproductive performance, and productivity. although supplemental diets for wild ruminants are commercially available, few have been evaluated in controlled intake and digestion trials. voluntary intake, digestive efficiency, nitrogen retention, and gross energy utilization of pronghorn (antilocapra americana), mule deer (odocoileus hemionus), mountain sheep (ovis can ...	1998	9732028
mp1: a mek binding partner that enhances enzymatic activation of the map kinase cascade.	signal transduction is controlled both by regulation of enzyme activation and by organization of enzymatic complexes with nonenzymatic adapters, scaffolds, and anchor proteins. the extracellular signal-regulated kinase (erk) cascade is one of several evolutionarily conserved mitogen-activated protein (map) kinase cascades important in the regulation of growth, apoptosis, and differentiation. a two-hybrid screen was conducted to identify nonenzymatic components of this signaling cascade that migh ...	1998	9733512
structures of sap-1 bound to dna targets from the e74 and c-fos promoters: insights into dna sequence discrimination by ets proteins.	sap-1 is a member of the ets transcription factors and cooperates with srf protein to activate transcription of the c-fos protooncogene. the crystal structures of the conserved ets domain of sap-1 bound to dna sequences from the e74 and c-fos promoters reveal that a set of conserved residues contact a gga core dna sequence. discrimination for sequences outside this core is mediated by dna contacts from conserved and nonconserved protein residues and sequence-dependent dna structural properties c ...	1998	9734357
isolation and characterisation of obligately anaerobic, lipolytic bacteria from the rumen of red deer.	two gram-positive, obligately anaerobic, lipolytic bacteria, isolates lip4 and lip5, were obtained from the rumen contents of juvenile red deer. these mesophilic bacterial strains were capable of hydrolysing the neutral lipids, tallow, tripalmitin and oliver oil, into their constituent free long-chain fatty acid and glycerol moieties. the latter compound was dissimilated by both isolates, with isolate lip4 producing propionate as the predominant product, while isolate lip5 produced acetate, etha ...	1998	9741118
bovine microsatellite loci are highly conserved in red deer (cervus elaphus), sika deer (cervus nippon) and soay sheep (ovis aries).	we tested 174 bovine microsatellite primer pairs for use in a primitive breed of sheep and two species of deer. of 173 markers, 127 (73.4%) gave a product in soay sheep (ovis aries) of which 54 (42.5%) were polymorphic. one hundred and twenty-nine of 174 (74.1%) markers gave a product in red deer (cervus elaphus) of which 72 (55.8%) were polymorphic. in sika deer (cervus nippon) 126 of 171 (73.7%) microsatellite primers gave a product with 47 (37.3%) polymorphic. the proportion of bovine microsa ...	1998	9745670
pulsatile luteinizing hormone secretion in the ovariectomized, thyroidectomized red deer hind following treatment with dopaminergic and opioidergic agonists and antagonists.	two experiments were conducted to determine whether dopaminergic or opioidergic pathways are modulated by thyroid gland secretions for seasonal suppression of lh secretion in red deer hinds. ovariectomized (n = 5) or ovariectomized and thyroidectomized (n = 4) hinds, treated with estradiol implants, received the dopamine agonist bromocriptine or the antagonist sulpiride during pulse bleeds in july (breeding season) and october (nonbreeding season). comparison of july and october mean plasma lh c ...	1998	9746749
alpha2-heremans schmid glycoprotein inhibits insulin-stimulated elk-1 phosphorylation, but not glucose transport, in rat adipose cells.	alpha2-heremans schmid glycoprotein (alpha2-hsg) is a member of the fetuin family of serum proteins whose biological functions are not completely understood. there is a consensus that alpha2-hsg plays a role in the regulation of tissue mineralization. however, one aspect of alpha2-hsg function that remains controversial is its ability to inhibit the insulin receptor tyrosine kinase and the biological actions of insulin. interestingly, some studies suggest that alpha2-hsg differentially inhibits ...	1998	9751494
hypotonicity activates transcription through erk-dependent and -independent pathways in renal cells.	acute hypotonic shock (50% dilution of medium with sterile water, but not with isotonic nacl) activated the extracellular signal response kinase (erk) mitogen-activated protein (map) kinases in renal medullary cells, as measured by western analysis with a phospho-erk-specific antibody and by in vitro kinase assay of epitope-tagged erks immunoprecipitated from stable ha-erk transfectants. hypotonicity also activated the transcription factor and erk substrate elk-1 in a partially pd-98059-sensitiv ...	1998	9755064
phenotypic and phylogenetic characterization of ruminal tannin-tolerant bacteria.	the 16s rrna sequences and selected phenotypic characteristics were determined for six recently isolated bacteria that can tolerate high levels of hydrolyzable and condensed tannins. bacteria were isolated from the ruminal contents of animals in different geographic locations, including sardinian sheep (ovis aries), honduran and colombian goats (capra hircus), white-tail deer (odocoileus virginianus) from upstate new york, and rocky mountain elk (cervus elaphus nelsoni) from oregon. nearly compl ...	1998	9758806
oxidant stress incites spreading of macrophages via extracellular signal-regulated kinases and p38 mitogen-activated protein kinase.	cultured macrophages exhibit spreading in response to external stimuli. it is relevant to in vivo morphologic changes of macrophages during extravasation, migration, and differentiation. the present study was performed to elucidate molecular mechanisms that regulate spreading of macrophages. redox is a crucial factor that modulates a wide range of cell function. we found that macrophages undergo spreading in response to oxidant stress caused by hydrogen peroxide or an oxidant generating agent me ...	1998	9759878
nociception activates elk-1 and sap1a following expression of the orl1 receptor in chinese hamster ovary cells.	nociceptin stimulation of the orl1 receptor expressed in chinese hamster ovary (cho) cells results in the activation of the extracellular signal regulated kinases erk1 and erk2. erk1/erk2 activation is inhibited by pertussis toxin, the mek inhibitor pd 98059 and by transient expression of alpha-transducin, indicating that orl1 up-regulation of these kinases occurs as a consequence of the release of the g-protein betagamma complex following the activation of pertussis-toxin sensitive galphai-fami ...	1998	9760105
cloning and characterization of gets-1, a goldfish ets family member that functions as a transcriptional repressor in muscle.	an ets transcription factor family member, gets-1, was cloned from a goldfish retina cdna library. gets-1 contains a conserved ets dna-binding domain at its n-terminus and is most similar to ternary complex factor (tcf) serum-response-factor protein-1a (sap-1a). gets-1 is expressed in many tissues, but is enriched in retina and brain. as with the tcfs sap-1a and ets-related protein (erp), overexpression of the gets-1 promoter suppresses nicotinic acetylcholine receptor epsilon-subunit gene expre ...	1998	9761723
extracellular signal-regulated kinase activity is sustained early during human cytomegalovirus infection.	expression of many early viral genes during human cytomegalovirus (hcmv) infection is dependent on cellular transcription factors. several immediate-early and early viral promoters contain dna binding sites for cellular factors such as creb, ap-1, serum response factor, and elk-1, and these transcription factors can be activated by phosphorylation via the cellular mitogen-activated protein kinase (mapk) signal transduction cascade. to determine if the extracellular signal-regulated mapks, erk1 a ...	1998	9765464
rsk-2 activity is necessary for epidermal growth factor-induced phosphorylation of creb protein and transcription of c-fos gene.	activation by growth factors of the ras-dependent signaling cascade results in the induction of p90 ribosomal s6 kinases (p90(rsk)). these are translocated into the nucleus upon phosphorylation by mitogen-activated protein kinases, with which p90(rsk) are physically associated in the cytoplasm. in humans there are three isoforms of the p90(rsk) family, rsk-1, rsk-2, and rsk-3, which are products of distinct genes. although these isoforms are structurally very similar, little is known about their ...	1998	9770464
the traf family of signal transducers mediates nf-kappab activation by the trance receptor.	tumor necrosis factor (tnf)-related activation-induced cytokine (trance), a member of the tnf family expressed on activated t-cells, bone marrow stromal cells, and osteoblasts, regulates the function of dendritic cells (dc) and osteoclasts. the trance receptor (trance-r), recently identified as receptor activator of nf-kappabeta (rank), activates nf-kappab, a transcription factor critical in the differentiation and activation of those cells. in this report we identify the tnf receptor-associated ...	1998	9774460
alpha-ketoglutarate dehydrogenase in alzheimer brains bearing the app670/671 mutation.	alzheimer's disease (ad) is associated with a striking reduction in the activity of the alpha-ketoglutarate dehydrogenase complex (kgdhc). the deficiency occurs in brains from ad patients of undefined etiology, and in fibroblasts from both sporadic and familial ad cases. to further assess the nature of the abnormality of kgdhc in ad, kgdhc activities and immunoreactivities were analyzed in brains from ad patients bearing the swedish app670/671 mutation. this gene defect causes overproduction of ...	1998	9778267
extracellular signal-regulated kinase (erk) controls immediate early gene induction on corticostriatal stimulation.	activity-dependent changes in neuronal structure and synaptic remodeling depend critically on gene regulation. in an attempt to understand how glutamate receptor stimulation at the membrane leads to gene regulation in the nucleus, we traced intracellular signaling pathways targeting dna regulatory elements of immediate early genes (iegs). for this purpose we used an in vivo electrical stimulation of the glutamatergic corticostriatal pathway. we show that a transient activation of extracellular s ...	1998	9786988
an evaluation of rapd fragment reproducibility and nature.	random amplified polymorphic dna (rapd) fragment reproducibility was assayed in three animal species: red deer (cervus elaphus), wild boar (sus scrofa) and fruit fly (drosophila melanogaster). ten 10-mer primers (operon) were tested in two replicate reactions per individual under different stringency conditions (annealing temperatures of 35 degrees c or 45 degrees c). two estimates were generated from the data: autosimilarity, which tests the reproducibility of overall banding patterns, and band ...	1998	9787445
isolation, characterization and localization of glycosaminoglycans in growing antlers of wapiti (cervus elaphus).	glycosaminoglycans were isolated from the four sections (tip, upper, middle and base) of the main beam of growing antlers of wapiti (cervus elaphus) by papain digestion and deae-sephacel chromatography. chondroitin sulfate was the major glycosaminoglycan in each section of antler accounting for, on average, 88% of the total uronic acid. the yield of chondroitin sulfate liberated from the tissue was approximately 6-fold greater in the cartilaginous (tip and upper) sections than in the bony (middl ...	1998	9787796
tissue migration of elaphostrongylus spp. in guinea pigs (cavia porcellus).	third-stage larvae of elaphostrongylus cervi, originating from red deer (cervus elaphus), first reached the central nervous system (cns) of guinea pigs (cavia porcellus) 11 days postinfection (dpi). neurologic signs were seen between 11 and 62 dpi in 4 of a total of 18 infected guinea pigs killed up to 112 dpi. animals showing signs had 3 or more larvae in the cns. only 1, of a total of 1,114 larvae recovered, had developed to the fourth stage at 40 dpi. a direct tissue migration by third-stage ...	1998	9794639
the human elk-1 gene family: the functional gene and two processed pseudogenes embedded in the igh locus.	elk-1 is a transcription factor whose activation by several mitogen-activated protein kinases (mapks) mediates the immediate early responses of the c-fos promoter to growth factors and other stimuli. here, we report the structure of the human elk-1 gene, which we have localized about 6.5kb upstream of the properdin gene on the x chromosome. the coding sequence is interrupted by four introns; two additional introns lie within the 5' untranslated region. we have also found two elk-1-related proces ...	1998	9795224
a constitutively active and nuclear form of the map kinase erk2 is sufficient for neurite outgrowth and cell transformation.	mitogen-activated protein (map) kinases are ubiquitous components of many signal transduction pathways. constitutively active variants have been isolated for every component of the extracellular-signal-regulated kinase 1 (erk1) and erk2 map kinase pathway except for the erk itself.	1998	9799732
signal transduction pathways activated by ret oncoproteins in pc12 pheochromocytoma cells.	gene alterations in the ret proto-oncogene, which encodes a receptor tyrosine kinase, have been found to associate with several human diseases. in this study, we showed that induction of the vgf promoter activity is a good molecular indicator for ret activation in pc12 cells, a rat pheochromocytoma cell line. we demonstrated that all forms of ret oncoprotein, including ret chimeric oncoproteins found in human papillary thyroid carcinomas (ret/ptc) as well as ret oncoproteins found in patients wi ...	1998	9478934
shear stress activates p60src-ras-mapk signaling pathways in vascular endothelial cells.	the aim of this study was to elucidate the upstream signaling mechanism that mediates the fluid shear stress activation of mitogen-activated protein kinases (mapks), including c-jun nh2-terminal kinase (jnk) and extracellular signal-regulated kinases (erks), in vascular endothelial cells (ecs). our results indicate that p60src is rapidly activated by fluid shear stress in bovine aortic endothelial cells (baecs). shear stress induction of the hemagglutinin (ha) epitope-tagged ha-jnk1 and the myc ...	1998	9484987
welfare of hunted red deer.		1998	9491532
[the occurrence of lumbar paralysis in goats and the appearance of elaphostrongylus cervi in red deer in the canton ticino].	the aim of the study was to examine the incidence of lumbar paralysis in goats and the possible role of elaphostrongylus cervi as an agent. for this reason questionnaires concerning the clinical pictures and the incidence of lumbar paralysis were sent to 200 owners of goats and to 9 veterinarians. the study showed that the symptoms of lumbar paralysis had been observed for many years mainly during the winter. most of the animals suffering from lumbar paralysis originated from the valley of leven ...	1998	9492579
eph receptors discriminate specific ligand oligomers to determine alternative signaling complexes, attachment, and assembly responses.	eph family receptor tyrosine kinases (including epha3, ephb4) direct pathfinding of neurons within migratory fields of cells expressing gradients of their membrane-bound ligands. others (ephb1 and epha2) direct vascular network assembly, affecting endothelial migration, capillary morphogenesis, and angiogenesis. to explore how ephrins could provide positional labels for cell targeting, we tested whether endogenous endothelial and p19 cell ephb1 (elk) and ephb2 (nuk) receptors discriminate betwee ...	1998	9499402
premature luteal regression induced by equine chorionic gonadotropin and estrogen is suppressed by administration of exogenous interferon in red deer (cervus elaphus).	superovulation of red deer hinds with ecg causes premature luteal regression by inducing follicular hypersecretion of estrogen that activates the luteolytic mechanism. six groups of hinds (n = 8 per group) were treated with progesterone-impregnated intravaginal controlled internal drug-releasing (cidr) devices for 14 days to synchronize estrus (cidr device withdrawal = day 0). group 1 served as controls; group 2 received an i.m. injection of 1200 iu ecg at -72 h; group 3 received similar ecg tre ...	1998	9472932
one-sample pregnancy diagnosis in elk using fecal steroid metabolites.	recent research has demonstrated the potential of pregnancy diagnosis in elk (cervus elaphus nelsoni) using immunoassays of fecal steroid concentration. however, multiple samples are required to insure accurate results, limiting its utility for free-ranging animals. we attempted to develop an accurate one-sample pregnancy diagnosis using 153 fecal samples that were collected from free-ranging, radio-collared, adult female elk in yellowstone national park (wyoming, usa) and from captive elk maint ...	1998	9476233
the kinase suppressor of ras (ksr) modulates growth factor and ras signaling by uncoupling elk-1 phosphorylation from map kinase activation.	the ras gtpase plays an essential role in many cellular signal transduction events. activation of the mitogen activated protein (map) kinase is a primary consequence of ras activation and plays a key role in mediating ras signal transduction. a novel kinase, ksr, has recently been functionally isolated as a positive regulator of ras signaling in caenorhabditis elegans vulval induction and drosophila photoreceptor differentiation. we have examined the effect of ksr on growth factor and ras-induce ...	1998	9501093
differential targeting of map kinases to the ets-domain transcription factor elk-1.	the activation of map kinase (mapk) signal transduction pathways results in the phosphorylation of transcription factors by the terminal kinases in these cascades. different pathways are activated by mitogenic and stress stimuli, which lead to the activation of distinct groups of target proteins. the ets-domain transcription factor elk-1 is a substrate for three distinct classes of mapks. elk-1 contains a targeting domain, the d-domain, which is distinct from the phosphoacceptor motifs and is re ...	1998	9501095
three-dimensional matrix primes mesangial cells to down-regulation of alpha-smooth muscle actin via deactivation of carg box elements.	prolonged culture of mesangial cells forms multifocal nodular structures, termed "hillocks," composed of cells and extracellular matrix (ecm), which may mimic the situation in the glomerular mesangium. mesangial cells incorporated in hillocks show repressed expression of alpha-smooth muscle actin, a marker of mesangial cell activation/dedifferentiation. the aim of this study is to elucidate molecular mechanisms involved in this phenomenon, focusing on the activity of carg box elements located in ...	1998	9507215
synergistic increase in c-fos expression by simultaneous activation of the ras/raf/map kinase- and protein kinase a signaling pathways is mediated by the c-fos ap-1 and sre sites.	expression of the c-fos proto-oncogene is induced by numerous stimuli some of which are transmitted through the ras/raf/map kinase or the camp-dependent protein kinase (pka) pathways. the effect of cell-specific interactions between these pathways on c-fos expression was investigated by exposing quiescent nih3t3 cells to serum, forskolin, or a combination. co-stimulation with serum and forskolin resulted in a more than additive increase in c-fos transcription. synergistic increase in c-fos promo ...	1998	9512670
transforming growth factor-beta1 and protein kinase c synergistically activate the c-fos serum response element in myocardial cells.	we previously reported that transforming growth factor-beta1 (tgf-beta1) potentiated alpha1-adrenergic and stretch-induced c-fos mrna expression and norepinephrine (ne)-induced amino acid incorporation in rat cultured myocardial cells (mcs). in the present study, we attempted to explore the mode of tgf-beta1 action for c-fos gene expression in mcs. in the transient transfection assay, tgf-beta1 potentiated ne- or 12-o-tetradecanoylphorbol-13-acetate (tpa)-activated c-fos promoter/enhancer, but n ...	1998	9515031
tumor necrosis factor-alpha induces interleukin-6 production and integrin ligand expression by distinct transduction pathways.	tumor necrosis factor-alpha (tnf-alpha) is a pleiotropic cytokine that elicits a large number of biological effects. however, the intracellular signaling mechanisms that are responsible for the tnf-alpha effects remain largely unknown. we have previously demonstrated that cultured mouse sertoli cells, after tnf-alpha treatment, increase the surface expression of adhesion molecules such as intercellular adhesion molecule-1 (icam-1) and vascular cell adhesion molecule-1 (vcam-1) and interleukin-6 ...	1998	9516459
population dynamics of norwegian red deer: density-dependence and climatic variation.	we present a model on plant-deer climate interactions developed for improving our understanding of the temporal dynamics of deer abundance and, in particular, how intrinsic (density-dependent) and extrinsic (plants, climate) factors influence these dynamics. the model was tested statistically by analysing the dynamics of five norwegian red deer populations between 1964 and 1993. direct and delayed density-dependence significantly influenced the development of the populations: delayed density-dep ...	1998	9523435
rapid activation of jnk1 in uv-b irradiated epidermal keratinocytes.	jun n-terminal kinase (jnk1) is a member of a family of stress-activated protein kinases which are activated by many forms of stress including uv radiation, resulting in the phosphorylation of c-jun, atf-2, elk-1 and p53. as uv-b radiation is mainly responsible for ultraviolet (uv)-induced skin cancers, we chose to elucidate jnk1 activation in keratinocytes which represent a uv-relevant cell system. we have demonstrated rapid activation of jnk1 in a keratinocyte cell line, c50, in response to mu ...	1998	9525748
aging-related deficiency of cd28 expression in cd4+ t cells is associated with the loss of gene-specific nuclear factor binding activity.	changes in t cell populations and concomitant perturbation of t cell effector functions have been postulated to account for many aging-related immune dysfunctions. here, we report that high frequencies of cd28(null) cd4+ t cells were found in elderly individuals. because deviations in the function of these unusual cd4+ t cells might be directly related to cd28 deficiency, we examined the molecular basis for the loss of cd28 expression in cd4+ t cells. in reporter gene bioassays, the minimal prom ...	1998	9525915
the cyclic adenosine monophosphate-dependent protein kinase (pka) is required for the sustained activation of mitogen-activated kinases and gene expression by nerve growth factor.	induction of neuronal differentiation of the rat pheochromocytoma cell line, pc12 cells, by nerve growth factor (ngf) requires activation of the mitogen-activated protein (map) kinase or extracellular signal-regulated kinase (erk). camp-dependent protein kinase (protein kinase a (pka)) also can induce differentiation of these cells. like ngf, the ability of pka to differentiate pc12 cells is associated with a sustained activation of erks. here we show that maximal sustained activation of erk1 by ...	1998	9525930
raf and fibroblast growth factor phosphorylate elk1 and activate the serum response element of the immediate early gene pip92 by mitogen-activated protein kinase-independent as well as -dependent signaling pathways.	previous studies have shown that a mitogen activated protein (map) kinase (mek)-independent signaling pathway is required by activated raf or fibroblast-derived growth factor (fgf) for the differentiation of rat hippocampal neuronal h19-7 cells. we now demonstrate that both raf and fgf similarly induce prolonged transcription and translation of the immediate early gene pip92 in the absence of activation of the map kinases (mapks) erk1 and erk2. to determine the mechanism by which this occurs and ...	1998	9528798
regulation of glucose transport and c-fos and egr-1 expression in cells with mutated or endogenous growth hormone receptors.	to identify mechanisms by which gh receptors (ghr) mediate downstream events representative of growth and metabolic responses to gh, stimulation by gh of c-fos and egr-1 expression and glucose transport activity were examined in chinese hamster ovary (cho) cells expressing mutated ghr. in cho cells expressing wild-type ghr(ghr(1-638)), gh stimulated the expression of c-fos and egr-1, and stimulated 2-deoxyglucose uptake, responses also mediated by endogenous ghr in 3t3-f442a cells. deletion of t ...	1998	9528972
evolutionarily conserved promoter region containing carg*-like elements is crucial for smooth muscle myosin heavy chain gene expression.	in recent years, significant progress has been made toward understanding skeletal muscle development. however, the mechanisms that regulate smooth muscle development and differentiation are presently unknown. to better understand smooth muscle-specific gene expression, we have focused our studies on the smooth muscle myosin heavy chain (smhc) gene, a highly specific marker of differentiated smooth muscle cells. the goal of the present study was to isolate and characterize the mouse smhc gene pro ...	1998	9529161
differentiation-stimulated activity binds an ets-like, essential regulatory element in the human promyelocytic defensin-1 promoter.	the human hnp-defensin-1 gene encodes a peptide antibiotic found exclusively in neutrophils and is key to elimination of microbes. expression is a marker for the granulocytic lineage and for certain stages of differentiation and is not known to be inducible in mature cells under physiological conditions. low level of transcription also occurs in hl-60 promyelocytic leukemia cells and is greatly activated upon drug-induced granulocytic maturation and by low doses of retinoic acid, in a strictly c ...	1998	9535850
urea activates ribosomal s6 kinase (rsk) in a mek-dependent fashion in renal mimcd3 cells.	urea activates a characteristic subset of signaling pathways in a tissue-specific fashion, including transcription of immediate early genes through activation of the mitogen-activated protein kinase (mapk), erk (extracellular signal-regulated kinase), and activation of its transcription factor substrate, elk-1. the ability of urea to activate the erk effector and pivotal regulatory kinase, ribosomal s6 kinase (rsk), was investigated in mimcd3 renal inner medullary collecting duct cells. urea upr ...	1998	9458825
the elk-1 ets-domain transcription factor contains a mitogen-activated protein kinase targeting motif.	the phosphorylation of transcription factors by mitogen-activated protein kinases (map) is a pivotal event in the cellular response to the activation of map kinase signal transduction pathways. mitogenic and stress stimuli activate different pathways and lead to the activation of distinct groups of target proteins. elk-1 is targeted by three distinct map kinase pathways. in this study, we demonstrate that the map kinase erk2 is targeted to elk-1 by a domain which is distinct from, and located n- ...	1998	9447967
nck recruitment to eph receptor, ephb1/elk, couples ligand activation to c-jun kinase.	eph family receptor tyrosine kinases signal axonal guidance, neuronal bundling, and angiogenesis; yet the signaling systems that couple these receptors to targeting and cell-cell assembly responses are incompletely defined. functional links to regulators of cytoskeletal structure are anticipated based on receptor mediated cell-cell aggregation and migratory responses. we used two-hybrid interaction cloning to identify ephb1-interactive proteins. six independent cdnas encoding the sh2 domain of t ...	1998	9430661
in vivo expression and regulation of elk-1, a target of the extracellular-regulated kinase signaling pathway, in the adult rat brain.	the transcription factor elk-1, a nuclear target of extracellular-regulated kinases (erks), plays a pivotal role in immediate early gene induction by external stimuli. notably, the degree of phosphorylation of elk-1 is tightly correlated with the level of activation of transcription of c-fos by proliferative signals. no data yet indicate the role of elk-1 in the adult brain in vivo. to address this question, we have analyzed in the present work (1) elk-1 mrna and protein expression in the adult ...	1998	9412502
neuroleptic treatment of red deer calves at weaning.	to determine whether neuroleptic drugs could be useful in reducing weaning stress in red deer, two groups of 30 calves were studied following separation from their dams for weaning at 3-4 months of age.	1998	16032030
observations of leptospirosis in farmed deer.	slaughterhouse and on-farm surveys were undertaken to investigate some aspects of leptospirosis (leptospira interrogans) in farmed deer in the lower north island of new zealand.	1998	16032037
epidemiology and pathogenesis of mycobacterium bovis infection of red deer (cervus elaphus) in new zealand.	this study was initiated to investigate aspects of the epidemiology, pathogenesis and transmission of tuberculosis in wild red deer, with the aim of determining whether this species may be considered a reservoir host of mycobacterium bovis in new zealand.	1998	16032039
the efficacy of a pour-on formulation of moxidectin in young red and wapiti-hybrid deer.	to measure the efficacy of a pour-on formulation of moxidectin against lungworm and abomasal parasites in weaner wapiti x red deer and to compare this with its efficacy in weaner red deer.	1998	16032046
a survey of internal parasites and parasite control on north island deer farms.	to evaluate internal parasite control practices and their effectiveness, and to investigate relationships between indices of parasitism and production outcomes by analysis of data collected during a deer herd health and production profiling project.	1998	16032051
activities and social relationships of red deer at pasture.	to provide baseline data for research into behaviourally appropriate management systems for deer.	1999	16032078
[attempt of deworming of red and fallow deer in deer farm with rintal premix].	rintal 2.4% premix (bayer) in single dose 7.5 mg of active substance on kg body weight was given to the calves of red and fallow deer. effectiveness of deworming against nematodes from family trichostrongylidae was 85.2% in red and 97.8% in fallow deer. against namatodes from genus trichocephalus the effectiveness was higher extending in red deer 98.1%, and in fallow deer 99.5%. anthelmintic was not effective against nematodes varestrongylus sagittatus and elaphostrongylus cervi.	1999	16888990
[character identification of 12 kinds of pilose antler medicinal materials].	in this paper, commercial medicinal materials of 12 kinds of pilose antler, cervus nippon, c. elaphus, c. albirostris, c. unicolor, c. eldihainanus, c. timorensis c. porinus, dama dama, rangifer farandus, alces alces, elaphurus davidianus, capreolus capreolus were compared and identified. a key and simple character illustration were listed.	1999	12571919
effects of preslaughter handling on the behaviour, blood biochemistry and carcases of farmed red deer.	eight groups of six red deer (four groups of males and four groups of females) were held overnight in an unfamiliar paddock, after which they were moved into a holding pen and slaughtered sequentially by stunning with a captive bolt pistol in a restraining pen. on entering the restraining pen, half the deer in each group were stunned immediately, but the other half were stunned after 10 minutes. deer spent much of the initial period in the holding pen standing stationary in 'alert' postures, but ...	1999	10189673
positive and negative regulatory elements in the upstream region of the rat cu/zn-superoxide dismutase gene.	cu/zn-superoxide dismutase (sod1) catalyses the dismutation of superoxide radicals and neutralizes the oxidative effects of various chemicals. deletion analysis of the upstream region of the rat sod1 gene revealed that the promoter contains a positive regulatory element (pre) and a negative regulatory element (nre), which encompass the regions from -576 to -412 and from -412 to -305 respectively from the site of initiation of transcription. these dna elements showed enhancer and silencer activit ...	1999	10191264
functional analysis of a mouse brain elk-type k+ channel.	members of the ether à go-go (eag) k+ channel subfamilies eag, erg, and elk are widely expressed in the nervous system, but their neural functions in vivo remain largely unknown. the biophysical properties of channels from the eag and erg subfamilies have been described, and based on their characteristic features and expression patterns, erg channels have been associated with native currents in the heart. little is known about the properties of channels from the elk subfamily. we have identified ...	1999	10191308
ternary complex factors elk-1 and sap-1a mediate growth hormone-induced transcription of egr-1 (early growth response factor-1) in 3t3-f442a preadipocytes.	in our search for transcription factors induced by gh, we have analyzed immediate early gene activation in a model of gh-dependent differentiation. here we describe the activation of early growth response factor-1 (egr-1) in gh-stimulated 3t3-f442a preadipocytes and the transcription factors responsible for its transactivation. binding activity of egr-1 in electrophoretic mobility shift assay (emsa) increased transiently 1 h after gh stimulation, accompanied by a concomitant increase in egr-1 mr ...	1999	10194767
bone morphogenetic protein 2 inhibits platelet-derived growth factor-induced c-fos gene transcription and dna synthesis in mesangial cells. involvement of mitogen-activated protein kinase.	bone morphogenetic proteins (bmps) play an important role in nephrogenesis. the biologic effect and mechanism of action of these proteins in the adult kidney has not yet been studied. we investigated the effect of bmp2, a member of these growth and differentiation factors, on mitogenic signal transduction pathways induced by platelet-derived growth factor (pdgf) in glomerular mesangial cells. pdgf is a growth and survival factor for these cells in vitro and in vivo. incubation of mesangial cells ...	1999	10196167
characterization of mhc-drb allelic diversity in white-tailed deer (odocoileus virginianus) provides insight into mhc-drb allelic evolution within cervidae.	although white-tailed deer (odocoileus virginianus) are one of north america's best studied mammals, no information is available concerning allelic diversity at any locus of the major histocompatibility complex in this taxon. using the polymerase chain reaction, single-stranded conformation polymorphism analysis, and dna sequencing techniques, 15 drb exon 2 alleles were identified among 150 white-tailed deer from a single population in southeastern oklahoma. these alleles represent a single locu ...	1999	10199919
environmental variation shapes sexual dimorphism in red deer.	sexual dimorphism results from dichotomous selection on male and female strategies of growth in relation to reproduction. in polygynous mammals, these strategies reflect sexual selection on males for access to females and competitive selection on females for access to food. consequently, in such species, males display rapid early growth to large adult size, whereas females invest in condition and early sexual maturity at the expense of size. hence, the magnitude of adult size dimorphism should b ...	1999	10200285
prevalence of borrelia burgdorferi in ixodes ricinus ticks in urban recreational areas of helsinki.	lyme borreliosis, an infection caused by the tick-borne spirochete borrelia burgdorferi, is a major health problem for populations in areas of endemicity in the northern hemisphere. in the present study we assessed the density of ticks and the prevalence of b. burgdorferi sensu lato among ticks in popular urban recreational areas of helsinki, finland. altogether 1,688 ixodes ricinus ticks were collected from five areas located within 5 km of the downtown section of helsinki, and 726 of them (303 ...	1999	10203487
involvement of protein kinase c and rho gtpase in the nuclear signalling pathway by transforming growth factor-beta1 in rat-2 fibroblast cells.	the transforming growth factor (tgf)-beta signal-transduction cascade from the cell membrane to the nuclear target is poorly characterised. here we report that treatment with tgf-beta1 induces the levels of endogenous c-fos mrna in rat-2 fibroblast cells. in addition, by transient transfection analysis, tgf-beta1 was shown to stimulate c-fos serum response element (sre)-driven reporter gene activity in a dose- and time-dependent manner, suggesting that sre is one of the nuclear targets of tgf-be ...	1999	10206347
effect of the method of capture on the haematology and blood chemistry of red deer (cervus elaphus).	haematological and plasma biochemical constituents can be affected during capture and handling operations in wildlife. the present study compares the blood constituents of 40 red deer (cervus elaphus) captured using two different methods: 20 were captured by physical means, using long nets and box traps, and 20 by chemical means, using a mixture of xylazine and ketamine. significant differences were found in the haemogram: red blood cell count, packed cell volume, haemoglobin concentration, and ...	1999	10208884
a mitochondrial control region and cytochrome b phylogeny of sika deer (cervus nippon) and report of tandem repeats in the control region.	sika deer (cervus nippon temminck) are endemic to mainland and insular asia. numerous subspecies have been named, but they are not quantitatively well defined. portions of the mitochondrial cytochrome b gene (450 bp) and control region (512 bp) were sequenced from 28 individuals belonging to five sika subspecies and two cervus elaphus subspecies. phylogenetic trees constructed using these sequences clearly demonstrated that sika are monophyletic with respect to c. elaphus. a survey of variation ...	1999	10222160
introgression through rare hybridization: a genetic study of a hybrid zone between red and sika deer (genus cervus) in argyll, scotland.	in this article we describe the structure of a hybrid zone in argyll, scotland, between native red deer (cervus elaphus) and introduced japanese sika deer (cervus nippon), on the basis of a genetic analysis using 11 microsatellite markers and mitochondrial dna. in contrast to the findings of a previous study of the same population, we conclude that the deer fall into two distinct genetic classes, corresponding to either a sika-like or red-like phenotype. introgression is rare at any one locus, b ...	1999	10224266
production, characterization, and uses of monoclonal antibodies against recombinant nucleoprotein of elk coronavirus.	this is the first report of the production of monoclonal antibodies against elk coronavirus. the nucleoprotein gene of elk coronavirus was amplified by pcr and was cloned and expressed in a prokaryotic expression vector. recombinant nucleocapsid protein was used to immunize mice for the production of hybridomas. twelve hybridomas that produced monoclonal antibodies against the nucleocapsid protein of elk coronavirus were selected by an indirect fluorescent-antibody test, an enzyme-linked immunos ...	1999	10225833