Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| neuropeptides in sensory structures of nematodes. | the peptidergic innervation of sensory structures in two species of nematodes, ascaris suum (order ascaridida, family ascarididae) and cystidicola farionis (order spirurida, family cystidicolidae) was studied. immunocytochemical methods were used for localization of fmrf-amide-like neuropeptides in the nervous system. immunoreactivity to fmrf-amide, rf-amide and salmf-amide was detected in the central nervous system of the species studied, and also in the cephalic papillary nerves, in axons of t ... | 1993 | 8493844 |
| [effect of thromboxane receptor antagonist, bay u 3405 on the late phase pulmonary response in guinea pigs]. | the effect of the specific thromboxane (tx)a2 receptor antagonist, bay u 3405 on the late asthmatic response (lar) was investigated in guinea pigs which are sensitized actively with ascaris suum antigen. respiratory resistance (rrs) of awake guinea pigs was measured by 30 hz oscillation method for 6 hours after antigen challenge. %rrs was calculated as the increase rate of rrs to baseline rrs (pre-challenge value). ten mg/kg of bay u 3405 or vehicle (0.5% methyl cellulose) was administered orall ... | 1993 | 8498891 |
| late asthmatic response causes peripheral airway hyperresponsiveness in dogs treated with metopirone. | to determine if late asthmatic response (lar) is associated with hyperresponsiveness of airway smooth muscle itself, we performed antigen challenge in dogs treated with metopirone. we studied the contractile response to acetylcholine (ach) in isolated bronchial and bronchiolar segments 8 h after either saline inhalation (the control group) or antigen challenge in dogs demonstrating immediate asthmatic response (iar) alone and in dogs demonstrating both iar and lar. airway responses to ascaris su ... | 1993 | 8508057 |
| the serine protease inhibitor family from ascaris suum: chemical determination of the five disulfide bridges. | chymotrypsin/elastase inhibitor-1 is a member of the ascaris family of serine protease inhibitors. it is characterized by five disulfide bridges in a polypeptide chain of 63 amino acids. the disulfide bridge pairing was resolved by cleavage at methionyl residues with cyanogen bromide followed by a combination of proteolytic digestions with glycyl endopeptidase, staphylococcal serine proteinase, and submandibular proteinase a. the peptides were separated on a reverse-phase hplc column. amino acid ... | 1993 | 8512322 |
| mechanisms of bronchoconstriction after allergen ingestion in sensitized guinea pigs. | we examined whether oral administration of allergen induced bronchoconstriction in sensitized guinea pigs and investigated the mechanisms of bronchoconstriction. the animals had been immunized intraperitoneally with a mixture of ascaris suum extract and silica gel, and exposed to ozone. they were then challenged with an oral dose of a. suum extract (6 mg/kg), and respiratory resistance (rrs) was measured up to 7 h. after oral administration of the allergen, an increase in rrs was observed. the m ... | 1993 | 8219783 |
| [the effect of experimental aerobic stabilization of swine slurry on survival of salmonella typhimurium and ascaris suum]. | laboratory aerobic mesophilic stabilisation (fermentation) of pig slurry reduced the survival time of s. typhimurium, compared with their prevalence in anaerobic excrements. the decimation time t90 for s. typhimurium, was 32 hours. the effect of aerobic stabilisation on the survival of nonembryoed eggs of the model helminth a. suum was lower than that observed in salmonella. after 54 hours of aerobic stabilization of slurry only 32% of eggs were devitalized. stabilized control group did not deve ... | 1993 | 8236636 |
| isolation of porcine ige, and preparation of polyclonal antisera. | high titre, ascaris specific, porcine reaginic antibody was induced by combining parasitic infestation and antigen challenge. the reagin was characterised as being heat labile at 56 degrees c, possessing long term skin sensitising activity, and as having physicochemical properties similar to ige in other species. porcine ige was purified by conventional immunochemical techniques, and an epsilon chain isolate prepared using sds-page gel excision and electro elution. antisera raised against the in ... | 1993 | 8236796 |
| inhibition of antigen-induced airway hyperresponsiveness by a thromboxane a2 receptor antagonist (aa-2414) in ascaris suum-allergic dogs. | we studied changes in airway responsiveness to acetylcholine (ach) after antigen inhalation in ascaris suum (a. suum)-allergic dogs. airway responsiveness was determined by obtaining a dose-response curve of lung resistance plotted against increasing concentrations of ach aerosol before and after inhalation of a. suum antigen. to determine the role of thromboxane a2 (txa2) in the airway response, we tested the effect of a txa2 receptor antagonist, aa-2414, in a. suum-allergic dogs. the procedure ... | 1993 | 8248544 |
| comparison of three enzyme immunoassays for diagnosis of dictyocaulus viviparus infection. | three enzyme-linked immunosorbent assays (elisa) that detect antibodies against dictyocaulus viviparus in cattle were compared for sensitivity, specificity and seroconversion after primary infection. these assays were (i) an indirect elisa with crude somatic antigens from adult d. viviparus (ca-elisa), (ii) an indirect elisa with purified antigens (sa-elisa) isolated from somatic antigens of adult d. viviparus and (iii) a competition elisa with antigen purified with anion chromatography in combi ... | 1993 | 8249247 |
| histological changes in hamster liver by es antigen of ascaris suum. | polyacrylamide gel electrophoresis with sds (page-sds) of the es antigens of a. suum revealed several protein molecules which differed from those obtained in es antigens of a. lumbricoides. nature of liver damage caused by es antigens of a. suum was studied in hamsters to find out the nature of damage and to compare with those caused by es antigens of a. lumbricoides. feeding of es antigens of a. suum was carried out in 7 hamsters for 75 days. after such feeding gross hepatic damage was noticed. ... | 1993 | 8262573 |
| doramectin, a new avermectin highly efficacious against gastrointestinal nematodes and lungworms of cattle and pigs: two studies carried out under field conditions in germany. | two field studies were carried out to confirm the efficacy of doramectin in cattle and pigs in europe. a total of 40 cattle harbouring naturally acquired infections of gastrointestinal nematodes, including ostertagia spp., oesophagostomum spp., cooperia spp., haemonchus spp., nematodirus spp. and trichuris spp., were allocated to a treated or a control group (20 animals/group), and 100 harbouring naturally acquired infections of gastrointestinal nematodes and lungworms, including oesophagostomum ... | 1993 | 8278344 |
| immediate allergic and nonallergic reactivity of the airways in ascaris skin-sensitive sheep. | immediate airway reactivity to ascaris suum allergen was compared with the responses to nonallergic airway challenge using histamine and acetylcholine in sheep with positive skin reactions to the allergen. in a control group of 6 sheep with negative skin test responses, allergen aerosol inhalation and allergen extract infusion only caused minor changes in mechanics of breathing as well as in arterial plasma histamine concentration. nearly 40% of the animals with a positive skin test responded to ... | 1993 | 8284521 |
| isoenzymatic pattern of glucose 6-phosphate dehydrogenase from ascaris suum. | the isoenzymatic pattern of glucose 6-phosphate dehydrogenase (abbreviation g6pd) from ascaris suum has been studied by vertical polyacrylamide gel (page) and horizontal starch gel electrophoresis. after polyacrylamide gel electrophoresis, two stained zones could be identified. one corresponded to tetrazolium oxidase activity; since this zone was stained even in the absence of glucose 6-phosphate (g6p), non-specific staining could be detected. in the other zone of activity, seven regularly-space ... | 1993 | 8288854 |
| developmentally regulated alternative splicing of a nematode type iv collagen gene. | a comparison of the genomic dna sequence that encodes the ascaris suum alpha 2(iv) collagen chain with the corresponding cdna sequence led to the identification of a putative exon that was not expressed in the cdna. the identification of this putative exon raised the possibility that transcripts of the alpha 2(iv) gene may undergo alternative splicing. we have used a reverse transcriptase-polymerase chain reaction assay to establish that such alternative splicing does indeed occur. our results s ... | 1994 | 8293875 |
| the tyrosine b10 hydroxyl is crucial for oxygen avidity of ascaris hemoglobin. | the parasitic nematode ascaris suum has a gene encoding a two-domain hemoglobin with remarkable oxygen avidity. the strong interaction with oxygen is a consequence of a particularly slow oxygen off-rate. the single polypeptide chain consists of two domains, each of which can be expressed separately in escherichia coli as a globin-like protein exhibiting oxygen binding characteristics comparable with the native molecule. site-directed mutagenesis was performed on the gene segment encoding domain ... | 1994 | 8300562 |
| interaction of mebendazole with tubulin from body wall muscle, intestine, and reproductive system of ascaris suum. | the binding of tritiated mebendazole, a benzimidazole anthelmintic, to tubulin derived from intestine, body wall muscle, and reproductive system of adult ascaris suum was examined and compared. mebendazole binding was resolved into specific and nonspecific binding and the binding affinity (ka) and maximum binding at infinite ligand concentration (bmax) determined. electron microscopy was performed to assess the tubulin in various tissues of a. suum quantitatively by observing the presence of mic ... | 1994 | 8308645 |
| antigen-induced airway hyperresponsiveness is associated with infiltration of eosinophils in lung tissue, but not with bronchoalveolar lavage eosinophilia or neutrophilia. | to examine the role of airway inflammation in airway hyperresponsiveness (ahr), we developed an animal model of ahr in guinea pigs and examined the histopathologic changes of these airways. guinea pigs were actively sensitized with dinitrophenylated ascaris suum extract and challenged with inhalation of the same extract. six and 24 h after antigen challenge, airway responsiveness to inhaled acetylcholine (ach), bronchoalveolar lavage fluid (balf) and lung histology were studied. airway responsiv ... | 1994 | 8260853 |
| bactericidal activity in the pig roundworm ascaris suum. | a potent, humoral, bactericidal activity against micrococcus luteus was discovered in pseudocoelomic fluid of the pig roundworm, ascaris suum. the activity, which was not bacteriolytic, was not due to lysozyme or to a dietary antibiotic. it was not inactivated by exposure to 100 degrees c, to low or high ph, or to ethanol. dialysis, electrophoresis and agar-diffusion experiments suggested that the main antibacterial activity in the fluid was associated with a basic substance of molecular weight ... | 1994 | 8144403 |
| effect of antigen on the glycoconjugate profile of tracheal secretions and the epithelial glycocalyx in allergic sheep. | to characterize the glycoconjugate composition of tracheal secretions and the apical glycocalyx of the tracheal epithelium under baseline conditions and after antigen challenge, sheep allergic to ascaris suum were intubated with a double-balloon nasotracheal tube to create a tracheal chamber. after an initial tracheal lavage, the animals were either exposed to intratracheally nebulized phosphate-buffered saline (pbs) (3 ml, n = 6) or a. suum antigen (251,000 protein nitrogen units in 3 ml of pbs ... | 1994 | 8151061 |
| characterization of identifiable neurones in the head ganglia of the parasitic nematode ascaris suum: a comparison with central neurones of caenorhabditis elegans. | intracellular recordings have been made from neurones in the head ganglia of ascaris. the neurones had low resting membrane potentials of -21 +/- 9 mv (n = 78) and a relatively high input resistance (e.g. 25 m omega for a 100 microns cell). in all cases the intracellular location of the recording electrode was verified by injection of the fluorescent marker, 5,6-carboxyfluorescein (cbxf). to ascertain whether or not the low membrane potential was due to impalement damage, the same neurone was re ... | 1994 | 8152859 |
| a nematode fmrfamide-like peptide, sdpnflrfamide (pf1), relaxes the dorsal muscle strip preparation of ascaris suum. | pf1 (sdpnflrfamide) is a fmrfamide-like peptide extracted from the free-living nematode panagrellus redivivus. here we show that this peptide causes a hyperpolarization of somatic muscle cells of the parasitic nematode ascaris suum and a relaxation of the somatic muscle strip preparation. we have assessed whether or not the relaxation of ascaris dorsal muscle strip by pf1 is due to (i) inhibition of the release of the excitatory neuromuscular junction transmitter acetylcholine (ach), (ii) potent ... | 1994 | 8159468 |
| effects of rolipram on responses to acute and chronic antigen exposure in monkeys. | the following study was performed to test the hypothesis that treatment with rolipram, a specific inhibitor of phosphodiesterase (pde) iv, should inhibit many pulmonary responses to acute and chronic antigen challenge in atopic monkeys by elevating intracellular camp and subsequently inhibiting leukocyte function. monkeys received subcutaneous injections of either vehicle (2% dmso) or 10 mg/kg of rolipram 1 h before exposure to ascaris suum antigen (ag). acute responses to ag, including bronchoc ... | 1994 | 8173755 |
| ksaymrfamide: a novel fmrfamide-related heptapeptide from the free-living nematode, panagrellus redivivus, which is myoactive in the parasitic nematode, ascaris suum. | in nematodes, fmrfamide-related peptides (farps) have been structurally characterised from the parasite, ascaris suum, and from two free-living species, panagrellus redivivus and caenorhabditis elegans. while both farps isolated from p. redivivus (pf1 and pf2) have been identified in c. elegans, the two heptapeptides isolated from a. suum (af1 and af2) have until recently been considered unique to this parasitic species. we have recently isolated af2 from p. redivivus and, during this study, an ... | 1994 | 8179635 |
| molecular cloning and expression of a cdna encoding glutathione s-transferase from ascaris suum. | 1994 | 8183318 | |
| the detection of akh/hrth-like peptides in ascaridia galli and ascaris suum using an insect hyperglycaemic bioassay. | evidence for the presence of adipokinetic hormone/hypertrehalosaemic hormone (akh/hrth)-like peptides in the parasitic nematodes ascaridia galli and ascaris suum has been obtained using insect bioassays which measure hyperglycaemic responses to peptides belonging to the akh/hrth family of insect hormones. a peptide fraction extracted from heads and tails of ascaridia galli evoked a dose-dependent hyperglycaemic response when injected into the cockroach, periplaneta americana. maximal bioactivity ... | 1994 | 8008462 |
| regulation of the ascaris major sperm protein (msp) cytoskeleton by intracellular ph. | the development and locomotion of the amoeboid sperm of the nematode, ascaris suum, depend on precise control of the assembly of their unique major sperm protein (msp) filament system. we used fluorescence ratio imaging of cells loaded with bcecf to show that intracellular ph (phi) is involved in controlling msp polymerization in vivo. spermatogenesis is marked by a cycle of msp assembly-disassembly-reassembly that coincides with changes in phi. in spermatocytes, which contain msp in paracrystal ... | 1994 | 8020106 |
| the epidemiology of ascaris suum infections in pigs in northern ireland, 1969-1991. | this paper presents the results of analysis of condemnations of pig livers due to cirrhosis which, in temperature climates, gives an accurate estimation of ascaris suum infections. over the 23 years analysed, a very highly significant upward trend in the prevalence of a. suum is demonstrated. the association between the prevalence and the mean air temperature over the early summer period is highlighted. thorough cleansing of pig units in early spring and at various times during the summer are su ... | 1994 | 8025848 |
| respiratory chain of the lung fluke paragonimus westermani: facultative anaerobic mitochondria. | the respiratory chain of adult paragonimus westermani, a lung fluke, was characterized in isolated mitochondria. the fluke mitochondria exhibited cyanide- and antimycin a-sensitive succinate oxidase activity at a rate of 16.8 nmol o2 min-1 mg-1 protein. the succinate oxidation was shown to be stimulated by adp and linked to the formation of membrane potential. the specific activities of oxidoreductases composing the succinate oxidase system, i.e., succinate-ubiquinone and succinate--cytochrome c ... | 1994 | 8031121 |
| hepatic vascular response to anaphylaxis in isolated canine liver. | we determined the vascular response to anaphylaxis in isolated canine livers perfused with autologous blood at a constant pressure via the portal vein, with hepatic artery ligation to simplify the vascular system. we also studied the validity of the double occlusion pressure (pdo) as a measure of the capillary pressure (pc) in the isolated canine liver. pdo was compared with pc measured using the traditional isogravimetric method (pc,i), and both parameters showed a strong correlation (pdo = 0.3 ... | 1994 | 8048632 |
| ultrastructural localization of pancreatic polypeptide- and fmrfamide immunoreactivities within the central nervous system of the nematode, ascaris suum (nematoda: ascaroidea). | a post-embedding immunogold technique has been used to examine the subcellular distribution of immunoreactivities to vertebrate pancreatic polypeptide (pp) and to the invertebrate peptide, fmrfamide within the central nervous system (cns) of the nematode, ascaris suum. gold labelling of peptide was localized exclusively over dense-cored vesicles within nerve cell bodies, nerve axons and nerve terminals of the main ganglia and nerve cords in the cns. double-labelling of peptides demonstrated an a ... | 1994 | 8052514 |
| biophysical model of the transcuticular excretion of organic acids, cuticle ph and buffer capacity in gastrointestinal nematodes. | a biophysical model was developed, using ascaris suum as a model gastrointestinal nematode, to provide quantitative perspectives into the microenvironmental ph within the water-filled, porous, negatively charged cuticle matrix of gastrointestinal nematodes. the central features of the model include (a) the constant rate of excretion of organic acid metabolites across the cuticle, (b) the relationship between cuticle ph and pka of the organic acids that determines the fraction of unionized and io ... | 1994 | 8069580 |
| molecular genealogy of some nematode taxa as based on cytochrome c and globin amino acid sequences. | we have begun to reconstruct the ancient history of the nematode phylum based on cytochrome c and globin amino acid sequences. the data suggest that the nematode ancestor diverged from a line leading to mammals about 1 billion years ago and that the most recent common ancestor of the extant species caenorhabditis elegans, trichostrongylus colubriformis, nippostrongylus brasiliensis, ascaris suum, and pseudoterranova decipiens lived about 550 my ago. the rhabditids and strongylids emerged as one ... | 1994 | 8075836 |
| [ascaridiasis zoonoses: visceral larva migrans syndromes]. | the syndrome of visceral larva migrans is a zoonotic disease due to the migration in human of nematodes larval, specially ascarid. since the larvae fail to complete their migrating cycle in humans, the diagnosis of toxocariasis infection remains only serologic. we have been able to demonstrate by the technique of agar diffusion and the western-blotting method that the etiology due to toxocara canis was twice as much frequent as the one due to toxocara cati in the syndrome of visceral and ocular ... | 1994 | 8076198 |
| primary and higher order structures of nematode (ascaris suum) mitochondrial trnas lacking either the t or d stem. | by fractionation using polyacrylamide gel electrophoresis and/or a preparative hybrid selection method employing solid-phase dna probes, we prepared and characterized mitochondrial trnas from the body wall muscle of ascaris suum, all of which are thought to lack either the t stem or the d stem from their gene sequences (okimoto, r., and wolstenholme, d. r. (1990) embo j. 10, 3405-3411). some of the partially purified trnas were appreciably aminoacylated with an extract of a. suum mitochondria. t ... | 1994 | 8077242 |
| immunocytochemical demonstration of peptidergic and serotoninergic components in the enteric nervous system of the roundworm, ascaris suum (nematoda, ascaroidea). | the localization and distribution of neuropeptides and an indoleamine (serotonin or 5-hydroxytryptamine) in the enteric nervous system (ens) of the pig roundworm, ascaris suum, have been determined by the application of an indirect immunofluorescence technique in conjunction with confocal scanning laser microscopy. whole-mount preparations of pharyngeal, intestinal and rectal regions were screened with antisera to 23 vertebrate peptides, 2 invertebrate peptides and serotonin (= 5-ht). positive i ... | 1994 | 7908737 |
| the molecular structure of the complex of ascaris chymotrypsin/elastase inhibitor with porcine elastase. | the intestinal parasitic worm, ascaris suum, produces a variety of protein inhibitors that defend the organism against the host's proteinases. eight different proteins from ascaris suum have been identified as inhibitors of serine proteinases, targeting chymotrypsin, elastase and trypsin. these inhibitors share 30-40% sequence identity with one another, but have virtually no sequence identity with members of any of the other families of serine proteinase inhibitors. | 1994 | 7922044 |
| engineering ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine b10. | the contribution to oxygen stabilization of a tyrosine residue in topological position (b10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (b10), (e7) and (e10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that of the nematode ascaris suum. kinetic and equilibrium experiments with the gaseous ligands oxygen and carbon monoxide show that indeed the introduction of tyrosine (b10), together with replacement of ... | 1994 | 7925944 |
| the motile major sperm protein (msp) of ascaris suum forms filaments constructed from two helical subfilaments. | the amoeboid motility nematode sperm is mediated by cytoskeletal filaments composed of major sperm protein (msp). we have used electron microscopy and image processing to show that msp filaments are constructed from two subfilament strands which are themselves formed from a helical arrangement of subunits. the subfilaments are based on left-handed helices of pitch 9 nm that then coil along right-handed helical tracks of pitch 22.5 nm to form filaments. the subfilaments appear to be indistinguish ... | 1994 | 7932742 |
| characterization of the n-acetyltransferases respectively responsible for arylalkylamine and diamine acetylation in ascaris suum. | 1994 | 7935612 | |
| the role of dityrosine formation in the crosslinking of cut-2, the product of a second cuticlin gene of caenorhabditis elegans. | a second cuticlin gene, cut-2, of the nematode caenorhabditis elegans, has been isolated and its genomic and cdna sequences determined. the gene codes for a component of cuticlin, the insoluble residue of nematode cuticles. conceptual translation of cut-2 reveals a 231-amino acid secreted protein which, like cut-1, begins with a putative signal peptide of 16 residues. the central part of the protein consists of 13 repetitions of a short hydrophobic motif, which is often degenerated with substitu ... | 1994 | 7935621 |
| nucleotide correlations that suggest tertiary interactions in the tv-replacement loop-containing mitochondrial trnas of the nematodes, caenorhabditis elegans and ascaris suum. | in the predicted secondary structures of 20 of the 22 trnas encoded in mitochondrial dna (mtdna) molecules of the nematodes, caenorhabditis elegans and ascaris suum, the t psi c arm and variable loop are replaced with a loop of 6 to 12 nucleotides: the tv-replacement loop. from considerations of patterns of nucleotide correlations in the central regions of these trnas, it seems highly likely that tertiary interactions occur within five sets of binary and ternary combinations of nucleotides that ... | 1994 | 7937159 |
| allergen-induced changes in bone marrow progenitors and airway responsiveness in dogs and the effect of inhaled budesonide on these parameters. | airway inflammation is implicated in the pathogenesis of the airway hyperresponsiveness in asthma. an increased production of inflammatory cell progenitors may contribute to asthmatic airway inflammation. although the number of circulating inflammatory cell progenitors in asthmatic subjects increases after allergen inhalation, no direct evidence exists for increased bone marrow progenitor production. we examined the effect of allergen inhalation on bone marrow progenitor production in seven dogs ... | 1994 | 7946389 |
| copper-zinc superoxide dismutase from ascaris suum (nematoda): purification and characterization. | copper-zinc superoxide dismutase from ascaris suum (nematoda) was purified in a new, more efficient, and faster manner. the process included differential centrifugation, fractionation with ammonium sulfate, and sodium dodecyl sulfate-polyacrylamide electrophoresis, yielding a 340-fold purification (specific activity of 47 units/mg). optimal storage conditions, optimal ph range, thermostability, molecular weight and ultraviolet-visible absorption spectrum of the enzyme are described, and a new en ... | 1994 | 7948773 |
| 2-methylbutyryl-coa: succinate acyl-coa transferase activity and function in ascaris suum muscle. | a branched-chain acyl-coa transferase activity which transfers coenzyme a from either 2-methylbutyryl or 2-methylvaleryl-coa to succinate is present in the muscle mitochondria from the intestinal nematode, ascaris suum. its physiological function is discussed. this activity appears to differ from the previously described acetyl-coa: propionate and propionyl-coa:succinate acyl-coa transferases on the basis of heat stability, substrate specificity and the requirement of a "factor" from boiled asca ... | 1994 | 7953070 |
| the fmrfamide-like neuropeptide af2 (ascaris suum) is present in the free-living nematode, panagrellus redivivus (nematoda, rhabditida). | available primary structural information suggests that the fmrfamide-related peptides (farps) from parasitic and free-living nematodes are different, and that free-living forms may not represent appropriate models for the study of the neurochemistry of parasitic forms in the laboratory. however, here we report the isolation and unequivocal identification of af2 (originally isolated from the parasite, ascaris suum) from acidified alcoholic extracts of the free-living species, panagrellus redivivu ... | 1994 | 7970891 |
| the effect of immunization of pigs with ascaris suum cuticle components on the development of resistance to parenteral migration during a challenge infection. | the development of immunity to ascaris suum was studied in pigs immunized with isolated cuticle fragments from a. suum second and third stage larvae (l2/l3) and adult worms, and compared with other methods that stimulate a strong protective response in pigs. a significant protective response was seen in animals immunized with isolated cuticle fragments from a. suum l2/l3 and adults, but it was less than that seen in animals inoculated with uv-irradiated eggs or naturally exposed to eggs on a dir ... | 1994 | 7975188 |
| sevoflurane and isoflurane protect against bronchospasm in dogs. | halothane and isoflurane have been shown to be effective in reversing bronchoconstriction; however, the effects of sevoflurane have not been well defined. we studied whether sevoflurane, compared with isoflurane, attenuates bronchospasm in dogs. | 1994 | 7978482 |
| structural characterization of an ascaris myoglobin. | globin was purified from the body wall of adults of the parasitic nematode ascaris suum. internal peptide fragments were sequenced and cdnas encoding a polypeptide of 154 amino acids isolated by polymerase chain reaction. the polypeptide lacks a signal sequence, identifying it as a cytosolic myoglobin-like species. the native protein is a dimer. the predicted amino acid sequence shares several unusual substitutions with other nematode globins. like the abundant pseudocoelomic a. suum hemoglobin ... | 1994 | 7982924 |
| novel regulation of pyruvate dehydrogenase phosphatase purified from anaerobic muscle mitochondria of the adult parasitic nematode, ascaris suum. | pyruvate dehydrogenase (pdhb) phosphatase has been purified to apparent homogeneity from mitochondria of the adult parasitic nematode, ascaris suum. the enzyme is a heterodimer of 89 and 50 kda, as judged by sds-polyacrylamide gel electrophoresis. it appeared to copurify with its substrate, pyruvate dehydrogenase (e1) and could be separated by chromatography on superose 12 in the presence of 0.5 m nacl. phosphatase activity was absolutely dependent on mg2+, with an apparent km of about 4 mm. in ... | 1994 | 7989326 |
| antigen-induced airway hyperresponsiveness is associated with airway tissue nep hypoactivity in rats. | in the present study, the role of sensory neuropeptides in the airway hyperresponsiveness (ahr) was investigated. first, the effect of the depletion of sensory neuropeptides by systemic capsaicin treatment on the ahr to acetylcholine (ach) induced by repeated antigenic challenge to sensitized rats was studied. we secondly investigated whether the neutral endopeptidase (nep) activity was altered at the antigen-induced ahr. male wistar rats were sensitized and repeatedly challenged with dnp-ascari ... | 1994 | 7990652 |
| leukotriene d4 receptor antagonism reduces airway hyperresponsiveness in monkeys. | airway hyperresponsiveness (ahr) and pulmonary inflammation are observations that are consistently associated with asthma and also occur in a well-characterized monkey model of asthma. the following study was performed to determine whether treatment with an ltd4 receptor antagonist, ici 198,615, could attenuate antigen-induced pulmonary inflammation and ahr in monkeys using the following protocol. on day 0, the pc200 (the concentration of methacholine (mch) that doubled respiratory system resist ... | 1994 | 8003852 |
| the mitochondrial ribosomal rna genes of the nematodes caenorhabditis elegans and ascaris suum: consensus secondary-structure models and conserved nucleotide sets for phylogenetic analysis. | the small- and large-subunit mitochondrial ribosomal rna genes (mt-s-rrna and mt-l-rrna) of the nematode worms caenorhabditis elegans and ascaris suum encode the smallest rrnas so far reported for metazoa. these size reductions correlate with the previously described, smaller, structurally anomalous mt-trnas of c. elegans and a. suum. using primer extension analysis, the 5' end nucleotides of the mt-s-rrna and mt-l-rrna genes were determined to be adjacent to the 3' end nucleotides of the trna(g ... | 1994 | 7528811 |
| the action of pyrantel as an agonist and an open channel blocker at acetylcholine receptors in isolated ascaris suum muscle vesicles. | the anthelmintic pyrantel is believed to act as an agonist at acetylcholine receptors on somatic muscle from the parasite ascaris suum. this study aimed to confirm this mode of action of pyrantel. single-channel recordings from muscle vesicles formed from the extrasynaptic region of the bag of somatic muscle cells of ascaris suum were made using the patch-clamp technique. pyrantel (0.03-100 microm) activated cation-selective channels with at least 2 conductance levels: main conductance 41 +/- 2. ... | 1994 | 7535704 |
| a carboxylesterase from the parasitic nematode ascaris suum homologous to the intestinal-specific ges-1 esterase of caenorhabditis elegans. | we have identified a carboxylesterase in a. suum that appears to be the homolog of the gut-specific c. elegans ges-1 enzyme. the a. suum esterase was purified and its n-terminal sequence found to be 50% identical to the c. elegans ges-1 protein. we have used isoelectric focusing analysis to demonstrate that, unlike the c. elegans ges-1 esterase, the a. suum enzyme is not restricted to the gut but is expressed in a wide range of tissues. | 1994 | 7553343 |
| effect of papaya latex against ascaris suum in naturally infected pigs. | an experiment was carried out to investigate the anthelmintic activity of papaya latex (carica papaya) against natural infection of ascaris suum in pigs. sixteen naturally infected pigs were, on the basis of faecal egg counts and body weight, allocated into four groups, each of four pigs. three groups (groups b, c, and d) were given papaya latex per os at dose levels of 2, 4, and 8 g of papaya latex per kg body weight, respectively. the fourth group (group a) served as a non-treated control. res ... | 1994 | 7706684 |
| sequence comparison between the flavoprotein subunit of the fumarate reductase (complex ii) of the anaerobic parasitic nematode, ascaris suum and the succinate dehydrogenase of the aerobic, free-living nematode, caenorhabditis elegans. | complex ii in adult mitochondria of the parasitic nematode, ascaris suum, exhibits high fumarate reductase activity and plays a key role in the anaerobic electron-transport observed in these organelles. in the present study, cdnas for the flavoprotein (fp) subunits of complex ii have been isolated, cloned and sequenced from both a. suum and the aerobic, free-living nematode, caenorhabditis elegans. additional sequence at the 3' end of the mrnas was determined by the rapid amplification of cdna e ... | 1994 | 7739664 |
| the prevalence of isospora suis and strongyloides ransomi in suckling piglets in the netherlands. | faecal samples from suckling piglets from 113 litters on 25 farms in the netherlands were examined to study the prevalence of gastrointestinal parasites, in particular strongyloides ransomi and isospora suis. s. ransomi was not found but i. suis was demonstrated in 17 of the 25 farms and in 41 out of 77 litters of these farms (53%). no other parasites were found. on the basis of these results, a longitudinal study on the incidence and significance of i. suis was performed on 10 farms (5 litters/ ... | 1994 | 7740744 |
| the interaction among granulocyte lipid mediators and the generation of oxygen radicals in antigen-induced airway hyperresponsiveness. | 1994 | 7771329 | |
| antigen (dnp-as)-induced allergic rhinitis model in guinea pigs. | in guinea pigs, an ige antibody was produced by intraperitoneal injection of antigen (dnp-as) containing al(oh)3 and booster inhalation of the antigen into the nasal cavity. experimental allergic rhinitis was induced by the perfusion of antigen solution into the nasal cavities of actively sensitized guinea pigs. severity of allergic rhinitis was assessed by determining release of histamine and leakage of dye into the nasal cavity. the antigen-induced release of histamine was significantly increa ... | 1994 | 7510462 |
| immune responses to nematode exoantigens: sensitizing antibodies and basophil histamine release. | high levels of ige and eosinophilia are found in both allergy and helminth infections, but allergic symptoms are rare in naturally acquired helminth infections. the interrelation of specific ige antibodies and in vitro basophil histamine release (hr) induced by exoantigens from the larval stages (l2/l3) of the nematodes toxocara canis and ascaris suum was examined in 148 patients visiting an outpatient clinic for parasitic diseases. the antigen sensitivity of the basophils was found to be depend ... | 1994 | 7521141 |
| immunorestorative effect of glucan immunomodulator on guinea pigs with experimental ascariosis. | the immunorestorative effect of glucan immunomodulator, combined with porcine immunoglobulin and zinc (gi) on t- and b-lymphocytes and peritoneal macrophage phagocytic ability was studied in guinea pigs with experimental ascariosis (ascaris suum) after a cyclophosphamide (cy)-evoked immunosuppression. during the migration phase of a. suum infection gi exerted a significant restorative effect on the cy-reduced percentage occurrence of t- and b-cell populations in the mesenteric, mediastinal and h ... | 1994 | 7810068 |
| the ca-activated chloride channel of ascaris suum conducts volatile fatty acids produced by anaerobic respiration: a patch-clamp study. | plasma membrane vesicles prepared from the bag region of the somatic muscle cell of the parasite ascaris suum contain a large conductance, voltage-sensitive, calcium-activated chloride channel. the ability of this channel to conduct a variety of carboxylic acids, a number of which are products of anaerobic respiration, was investigated using the patch-clamp technique and isolated inside-out patches of muscle membrane. the channel has a conductance of 140 ps in symmetrical 140 mm chloride. replac ... | 1994 | 7815451 |
| [study of the efficacy of sewage treatment systems for fluid waste on pig farms in slovakia]. | bacteriological, helminthological and physico-chemical examinations were carried out to investigate the technological procedure in three water treatment plants treating slurry from large-capacity pig farms (kosická polianka, spisské vlachy, vel'ký dur). our investigations were focused on the testing of effectiveness of mechanical, chemical and biological treatment system most frequently used in slovakia. our investigations revealed that water-treatment plants, operating on pig farms, fulfil only ... | 1994 | 7817500 |
| metabolism of af1 (knefirf-nh2) in the nematode ascaris suum. | 1994 | 7821552 | |
| peptides related to the diploptera punctata allatostatins in nonarthropod invertebrates: an immunocytochemical survey. | the allatostatins are a family of peptides isolated originally from the cockroach, diploptera punctata. related peptides have been identified in periplaneta americana and the blowfly, calliphora vomitoria. these peptides have been shown to be potent inhibitors of juvenile hormone synthesis in these species. a peptide inhibitor of juvenile hormone biosynthesis has also been isolated from the moth, manduca sexta; however, this peptide has no structural homology with the d. punctata-type allatostat ... | 1994 | 7822491 |
| identification and analysis of a cuticular collagen-encoding gene from the plant-parasitic nematode meloidogyne incognita. | the vast majority of proteins in the nematode cuticle are collagens. cuticular collagen-encoding genes (col) have been described for the animal parasites ascaris suum and haemonchus contortus and for the free-living caenorhabditis elegans. the proteins encoded by all these genes seem to have the same basic structure, indicating that there is a conserved subfamily of cuticular col in these nematodes. in this paper, we describe the identification and characterization of a cdna (lemmi 5) which corr ... | 1994 | 7828882 |
| effect of an inhaled corticosteroid on airway eosinophils and allergen-induced airway hyperresponsiveness in dogs. | the presence of airway eosinophils before allergen inhalation may contribute to the development of allergen-induced airway responses. we examined whether a reduction in airway eosinophil numbers before allergen inhalation as a result of inhalation of the corticosteroid budesonide would prevent allergen-induced airway hyperresponsiveness in seven dogs. acetylcholine airway responsiveness was measured before and 24 h after inhalation of ascaris suum allergen (10(-6)-10(-2) wt/vol) or its diluent o ... | 1994 | 7836134 |
| pyruvate dehydrogenase complexes from the equine nematode, parascaris equorum, and the canine cestode, dipylidium caninum, helminths exhibiting anaerobic mitochondrial metabolism. | the pyruvate dehydrogenase complex (pdc) has been purified to apparent homogeneity from 2 parasitic helminths exhibiting anaerobic mitochondrial metabolism, the equine nematode, parascaris equorum, and the canine cestode, dipylidium caninum. the p. equorum pdc yielded 7 major bands when separated by sds-page. the bands of 72, 55-53.5, 41 and 36 kda corresponded to e2, e3, e1 alpha and e1 beta, respectively. the complex also contained additional unidentified proteins of 43 and 45 kda. incubation ... | 1994 | 7870133 |
| supramolecular assemblies of the ascaris suum major sperm protein (msp) associated with amoeboid cell motility. | sperm of the nematode, ascaris suum, are amoeboid cells that do not require actin or myosin to crawl over solid substrata. in these cells, the role usually played by actin has been taken over by major sperm protein (msp), which assembles into filaments that pack the sperm pseudopod. these msp filaments are organized into multi-filament arrays called fiber complexes that flow centripetally from the leading edge of the pseudopod to the cell body in a pattern that is intimately associated with moti ... | 1994 | 7876359 |
| obtention of ovine ige from heterohybridoma. | mesenteric and bronchial lymph node cells from sheep immunized with ascaris suum antigens in combination with bordetella pertussis vaccine were fused with mouse myeloma cell lines, p3-x63-ag8.653, nso.u and ns1.1.ag1.1. one heterohybridoma cell line (ns1.1.ag1.1 x sheep) producing ovine immunoglobulin e was detected by the passive cutaneous anaphylaxis test. | 1994 | 7889039 |
| proteinase inhibitors in ascarida. | ascaris suum and a. lumbricoides are intestinal parasites that survive in a hostile hydrolytic environment they contain low-molecular-weight proteins that can inactivate most of the proteinases present in the worm's surroundings. jeffrey hawley, mark martzen and robert peanasky suggest that host specificity of these nematodes may be associated with the efficiency of their inhibitors. | 1994 | 15275429 |
| morphometric estimation of superoxide generation in allergen-induced airway hyperresponsiveness. | we previously reported that the airway hyperresponsiveness (ahr) that follows antigen challenge is mediated, in part, by the generation of reactive oxygen species. the purpose of this study was to provide quantitative morphologic evidence of oxygen radical production in the airways during antigen-induced ahr. | 1995 | 7898054 |
| metal ion activator effects on intrinsic isotope effects for hydride transfer from decarboxylation in the reaction catalyzed by the nad-malic enzyme from ascaris suum. | the mechanism of the oxidative decarboxylation reaction catalyzed by the nad-malic enzyme from ascaris suum has been examined with several different divalent metal ion activators and dinucleotide substrates. primary deuterium and tritium isotope effects have been obtained and, in combination with the partitioning ratios of the oxalacetate intermediate to malate and pyruvate, have been used to calculate commitment factors, intrinsic deuterium isotope effects on the hydride transfer step, and intr ... | 1995 | 7880820 |
| localization of diploptera punctata allatostatin-like immunoreactivity in helminths: an immunocytochemical study. | the nervous systems of helminths are predominantly peptidergic in nature, although it is likely that the full range of regulatory peptides used by these organisms has yet to be elucidated. attempts to identify novel helminth neuropeptides are being made using immunocytochemistry with antisera raised against peptides isolated originally from insects. one of these antisera was raised against allatostatin iii, a peptide isolated originally from the cockroach, diploptera punctata, and a member of a ... | 1995 | 7845717 |
| stage-specific isoforms of complex ii (succinate-ubiquinone oxidoreductase) in mitochondria from the parasitic nematode, ascaris suum. | complex ii from mitochondria of the adult parasitic nematode, ascaris suum, exhibits high fumarate reductase activity and plays a key role in the anaerobic electron transport observed in these organelles. in contrast, mitochondria isolated from free living second stage larvae (l2) of a. suum show much lower fumarate reductase activity than those from adults, whereas succinate dehydrogenase activities of mitochondria in both stages are comparable. in the present study, biochemical and antigenic p ... | 1995 | 7822332 |
| ascaris suum: characterization of transmural and hypodermal potentials. | electrophysiological measurements demonstrate that an electrochemical potential exists across the body wall of parasitic nematodes. the ionic dependence of this transmural electrical potential (etm) in the gastrointestinal nematode ascaris suum was investigated using conventional electrophysiological techniques. etm recorded from intact a. suum maintained in artificial pseudocoelomic fluid (apf) was -40 +/- 12 mv. this potential was more sensitive to external acetate than to na+, k+, or cl-, alt ... | 1995 | 7821415 |
| persistent airway hyperresponsiveness and histologic alterations after chronic antigen challenge in cats. | we studied the effect of chronic immune sensitization on the airway reactivity and associated cytologic and histologic alterations in initially nonatopic cats, a species that spontaneously develops idiopathic asthma. seven cats were sensitized by intramuscular injection of ascaris suum antigen (aa) for 4 wk, and four other cats served as sham controls. airway sensitization was demonstrated by an increased response to nebulized aa in sensitized animals (rl = 45.9 +/- 6.1 cm h2o/l/s, versus a base ... | 1995 | 7812551 |
| glycerophosphorylcholine, a component of both ascaris suum muscle and caenorhabditis elegans. | studies of the muscle phospholipid metabolism of ascaris suum suggest an effect of cholinergic drugs on the turnover of phosphatidylcholine and the generation of glycerophosphorylcholine (gpc). 31p-nuclear magnetic resonance (nmr) studies of helminths revealed the presence of a major peak that was assigned to gpc. the primary effect of the cholinergic drugs on the parasites' phosphate profile appeared to be on the level of gpc. in in vivo studies, decreases in internal gpc concentrations occurre ... | 1995 | 7776117 |
| genetic structure of midwestern ascaris suum populations: a comparison of isoenzyme and rapd markers. | isoenzyme and random amplified polymorphic dna (rapd) markers were used to characterize the genetics of geographic variation among population samples of ascaris suum from midwestern localities. independent estimates of fixation indices (fst) based on isoenzyme and rapd markers showed the same general patterns of differentiation and substantial statistical correlation (r = 0.70). of the total estimated gene diversity, 9.4% (isoenzyme) and 9.2% (rapd) was distributed among infrapopulations. geogra ... | 1995 | 7776123 |
| novel arylaminopyridazine-gaba receptor antagonists examined electrophysiologically in ascaris suum. | the structure-activity relationships of 35 novel derivatives of 2-(carboxypropyl)-3-amino-4-methyl-6-phenyl pyridazine (sr 95103) were examined as gamma-aminobutyric acid (gaba) antagonists in the flap preparation of the parasitic nematode, ascaris suum, using a two-microelectrode current-clamp technique. all but one of the potent antagonists displaced gaba dose-response curves to the right without reduction in the maximum response. the dissociation constants of the more potent competitive antag ... | 1995 | 7781700 |
| devitalization of bacterial and parasitic germs in sewage sludge during aerobic digestion under laboratory conditions. | the survival of 8 bacterial species (pseudomonas sp., salmonella sp., enterobacteriae, streptococcus sp., escherichia coli) was detected in municipal sewage sludge up to 37 hours of mesophilic aerobic digestion under laboratory conditions. the model strain enterococcus faecium ccm 4231 survived almost twice as long as the above-mentioned isolates. similar findings, regarding the viability of the microorganisms studied, were also determined during thermophilic aerobic digestion of municipal sewag ... | 1995 | 7793018 |
| acid-base catalytic mechanism and ph dependence of fructose 2,6-bisphosphate activation of the ascaris suum phosphofructokinase. | a form of phosphofructokinase (pfk) from ascaris suum desensitized to hysteresis in the reaction time course and atp allosteric inhibition has been used to study the activation by fructose 2,6-bisphosphate (f26p2) at varied ph in both reaction directions. in the direction of phosphorylation of f6p, v and v/kmgatp are constant over the ph range 6-9, while v/kf6p decreases at low ph, giving a pk value of 7.0, and at high ph, giving a pk of 8.9. v and v/kmgatp are insensitive to the presence of f26 ... | 1995 | 7794888 |
| dictyocaulus viviparus: nucleotide sequence of dv3-14. | 1995 | 7797366 | |
| ige regulation by nematodes: the body fluid of ascaris contains a b-cell mitogen. | nematode infection of human beings or laboratory animals leads to markedly increased levels of circulating ige, most of which is not specific to worm antigens. this phenomenon is known to be interleukin-4-dependent, but little is known about the mechanism of activation of the response. in an attempt to elucidate this mechanism, we have used a reductive approach with worm products rather than infections. in a previous article we showed that injection of the body fluid of the nematode ascaris yiel ... | 1995 | 7797793 |
| immunization with ascaris suum extract impairs t cell functions in mice. | the effect of an ascaris suum extract (asc) on several t cell functions was studied in mice immunized with asc and ovalbumin (oa) in complete freund's adjuvant. delayed-type hypersensitivity reactions following challenge with aggregated oa were markedly diminished in mice injected with oa plus a high dose of asc compared to oa-immunized animals. proliferation and il-2, ifn-gamma, il-4, and il-10 production in oa-stimulated lymph node cells from the oa + asc-immunized group were also inhibited. t ... | 1995 | 7743547 |
| alterations in polyamine levels of nematode, earthworm, leech and planarian during regeneration, temperature and osmotic stresses. | free-living nematodes, caenorhabditis elegans and dorylaimus fodori, contain putrescine and spermidine. putrescine, spermidine and spermine occur in the parasitic nematoda, ascaris suum, anisakis simplex and dirofilaria immitis. earthworms, eisenia foetida, tubifex hattai and pheretima communissima and the leech, hirudo nipponia (belonging to annelida) and the planarian, dugesia japonica (belonging to platyhelminthes) contain homospermidine and spermine in addition to putrescine and spermidine. ... | 1995 | 7749639 |
| the effect of the nematode peptides sdpnflrfamide (pf1) and sadpnflrfamide (pf2) on synaptic transmission in the parasitic nematode ascaris suum. | the action of two peptides isolated from the nematode panagrellus redivivus, pf1 (sdpnflrfamide) and pf2 (sadpnflrfamide) have been studied on synaptic transmission in the motornervous system of the parasitic nematode ascaris suum. intracellular recordings were made from ascaris somatic muscle cells and excitatory junction potentials (ejps) elicited by stimulation of the ventral nerve cord. the ejps were cholinergic as they were blocked by the ascaris nicotinic receptor antagonist, benzoquinoniu ... | 1995 | 7753584 |
| the aba-1 allergen of the parasitic nematode ascaris suum: fatty acid and retinoid binding function and structural characterization. | we report here on the structure and function of the aba-1 allergen protein of the parasitic nematode ascaris, the first nematode allergen to be characterized in detail. using the fluorescent fatty acid analog 11-(((5-(dimethylamino)-1-naphthalenyl)sulfonyl)amino)undecanoic acid (dauda), it was demonstrated that aba-1 is a fatty acid binding protein (fabp) with a high affinity for the fluorescent analog (8.8 x 10(-8) m) and for oleic acid in competition experiments (1.3 x 10(-8) m), with a single ... | 1995 | 7756301 |
| studies on the isolation and characterisation of a reaginic antibody in a cat. | a reaginic antibody has been demonstrated, by passive cutaneous anaphylaxis (pca), in the serum of a cat infected with the microfilariae of brugia pahangi. recipient cats and pigs were challenged with an extract of ascaris suum after either a four-hour or a 72-hour period of sensitisation. when the serum was heat treated at 56 degrees c it lost its pca activity. gel filtration of the serum revealed a pattern of positive pca fractions similar to that observed in other species. attempts to purify ... | 1995 | 7709065 |
| cardiovascular and hormonal responses to anaphylactic shock in the pig. | cardiovascular and hormonal responses to anaphylactic shock were evaluated in anaesthetized pigs sensitized by natural exposure to ascaris suum as verified by antibodies. in six animals with such antibodies, ascaris antigen injection produced a plasma histamine increase of 52 (42-196) fold (median and range; p < 0.05), while four pigs without such antibodies served as controls with only insignificant increases in histamine. in the anaphylactic group, two of the animals died during the investigat ... | 1995 | 7712695 |
| isolation and preliminary biological characterization of kpnfirfamide, a novel fmrfamide-related peptide from the free-living nematode, panagrellus redivivus. | a novel fmrfamide-related heptapeptide, lys-pro-asn-phe-ile-arg-phe-nh2 (kpnfirfamide), was isolated and characterized from acid ethanol extracts of the free-living nematode, panagrellus redivivus. whole-worm extracts contained > or = 9 pmol kpnfirfamide/g wet weight. a synthetic replicate of this peptide induced a rapid relaxation of tone and inhibited spontaneous contractility in isolated innervated and denervated body-wall muscle strips of the parasitic nematode, ascaris suum. kpnfirfamide (0 ... | 1995 | 7716079 |
| [survival of ascaris suum eggs in sewage treatment plant sludge]. | in the period between june 1992 and may 1993 the survival of nonembryonated eggs of a. suum was studied in two sludge drying beds of sewage treatment plants (stp) under different climatic-geografical conditions: stp michalovce in the east slovak lowland (elevation 111 m above sea-level) and stp poprad in the submontane area of the poprad valley (elevation 695 m). sludge drying beds of both sewage treatment plants (stp) showed different survival of eggs (fig. 1). in stp michalovce we detected a r ... | 1995 | 7716865 |
| the effects of the nematode peptide, kheylrfamide (af2), on the somatic musculature of the parasitic nematode ascaris suum. | af2 is an endogenous rfamide-like peptide from the parasitic nematode ascaris suum. the potent stimulatory effects of this peptide on the somatic musculature of ascaris strongly suggest that it may have an important role in the motornervous system. here we have investigated the possibility that af2 may elicit a stimulatory action on ascaris muscle by potentiating the actions of the excitatory cholinergic motonervous system either pre-synaptically, post-synaptically or both. in in vitro pharmacol ... | 1995 | 7724243 |
| allergen-induced oxygen radical release from bronchoalveolar lavage cells and airway hyperresponsiveness in dogs. | allergen inhalation causes airway hyperresponsiveness and airway inflammation in dogs. the purpose of this study was to determine whether allergen-induced airway hyperresponsiveness is associated with increases in oxygen radical production from bronchoalveolar lavage (bal) cells. a group of 10 random-source dogs were studied twice, 4 wk apart. on each occasion, acetylcholine (ach) airway responsiveness was measured before and 24 h after inhalation of ascaris suum or its diluent, followed by bal. ... | 1995 | 7735610 |
| the catalytic subunit of camp-dependent protein kinase from ascaris suum. the cloning and structure of a novel subtype of protein kinase a. | a complete cdna clone encoding the catalytic subunit of camp-dependent protein kinase of ascaris suum was constructed from two overlapping partial clones. the encoded sequence of 337 amino acids is 48% identical with the sequence of mouse c alpha subunit. approximately the same low similarity was found with the sequence of the c subunit from another nematode, caenorhabditis elegans. the n-terminal 14 amino acids and the myristoylation site of the mammalian protein are not contained in the enzyme ... | 1995 | 7556139 |
| loa loa: structural diversity of a 15-kda repetitive antigen. | a ladder antigen of loa loa was identified on western blots of all life cycle stages probed with loaisis sera. the smallest subunit has a relative m(r) of about 15 kda and larger subunits represent size increments of 15.0 kda. an 1800-bp genomic clone encoding this antigen was characterized further by restriction mapping. southern blot analysis, and nucleotide sequencing. the antigen is encoded by multiple copies of a gene, linked in tandem repeats of 396 bp, each of which encodes 132 amino acid ... | 1995 | 7556556 |
| the action of serotonin and the nematode neuropeptide ksaymrfamide on the pharyngeal muscle of the parasitic nematode, ascaris suum. | the pharyngeal component of the enteric nervous system of the parasitic nematode, ascaris suum exhibits immunoreactivity for serotonin (5-hydroxytryptamine or 5-ht) and for fmrfamide-like peptides. this paper describes the application of an in vitro pharmacological approach to investigate the functional role of 5-ht and fmrfamide-like peptides. the pharyngeal pumping behaviour of ascaris suum was monitored using a modified pressure transducer system which measures pharyngeal pressure changes and ... | 1995 | 7567105 |
| the effect of two types of diet on populations of ascaris suum and oesophagostomum dentatum in experimentally infected pigs. | this investigation compared the effect of two common types of diet on worm burdens and faecal egg counts of pigs inoculated with ascaris suum and oesophagostomum dentatum. the diets were both considered nutritionally sufficient for pigs of this age, and gave high and comparable growth rates. pigs were inoculated with both worm species at 14 weeks of age and slaughtered 3 or 12 weeks later. at 3 weeks, a. suum larvae were numerous in the small intestine of pigs fed ground barley plus protein supp ... | 1995 | 7567107 |
| actions of neurotoxins (bungarotoxins, neosurugatoxin and lophotoxins) on insect and nematode nicotinic acetylcholine receptors. | neurotoxins of natural origin have proved to be of considerable value in the isolation and characterization of vertebrate muscle and neuronal nicotinic acetylcholine receptors (nachrs). to date, they have been used less extensively in studies of invertebrate nachrs. here we examine how a variety of neurotoxins (the snake toxins alpha-bungarotoxin, alpha-bgt, and kappa-bungarotoxin, kappa-bgt, the molluscan toxin, neosurugatoxin, and the soft coral toxins, lophotoxin and bipinnatin-b) can be used ... | 1995 | 7570627 |
| survival of salmonellas and ascaris suum eggs in a thermophilic biogas plant. | in a continuous biogas plant, receiving manure from 200 dairy cows and 400 calves and young stock, survival of salmonellas and ascaris suum eggs was studied. the bacteria and parasite eggs were kept in filter sacs in the manure that had a temperature of 55 degrees c. no viable salmonellas or ascaris suum eggs could be found after 24h in the digester. survival of salmonellas and ascaris suum eggs was also studied in the manure pit where the manure was stored after digestion. the temperature in th ... | 1995 | 7572460 |