Publications

ethanol production by a new pentose-fermenting yeast strain, scheffersomyces stipitis ufmg-imh 43.2, isolated from the brazilian forest.	the ability of a recently isolated scheffersomyces stipitis strain (ufmg-imh 43.2) to produce ethanol from xylose was evaluated. for the assays, a hemicellulosic hydrolysate produced by dilute acid hydrolysis of sugarcane bagasse was used as the fermentation medium. initially, the necessity of adding nutrients (mgso(4) ·7h(2) o, yeast extract and/or urea) to this medium was verified, and the yeast extract supplementation favoured ethanol production by the yeast. then, in a second stage, assays u ...	2011	21626536
co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus for simultaneous biosynthesis of xylanase and laccase under solid-state fermentation.	co-cultivation of mutant penicillium oxalicum sau(e)-3.510 and pleurotus ostreatus mtcc 1804 was evaluated for the production of xylanase-laccase mixture under solid-state fermentation (ssf) condition. growth compatibility between mutant p. oxalicum sau(e)-3.510 and white rot fungi (p. ostreatus mtcc 1804, trametes hirsuta mtcc 136 and pycnoporus sp. mtcc 137) was analyzed by growing them on potato dextrose agar plate. extracellular enzyme activities were determined spectrophotometrically. under ...	2011	21642024
optimization of fibrolytic enzyme production by aspergillus japonicus c03 with potential application in ruminant feed and their effects on tropical forages hydrolysis.	fibrolytic enzyme production by aspergillus japonicus c03 was optimized in a medium containing agro-industrial wastes, supplemented with peptone and yeast extract. a 2(3) full factorial composite and response surface methodology were used to design the experiments and analysis of results. tropical forages were hydrolyzed by a. japonicus c03 enzymatic extract in different levels, and they were also tested as enzymatic substrate. optimal production to xylanase was obtained with soybean bran added ...	2011	21647681
responses of maize (zea mays l.) near isogenic lines carrying wsm1, wsm2, and wsm3 to three viruses in the potyviridae.	genes on chromosomes six (wsm1), three (wsm2) and ten (wsm3) in the maize (zea mays l.) inbred line pa405 control resistance to wheat streak mosaic virus (wsmv), and the same or closely linked genes control resistance to maize dwarf mosaic virus (mdmv) and sugarcane mosaic virus (scmv). near isogenic lines (nil) carrying one or two of the genes were developed by introgressing regions of the respective chromosomes into the susceptible line oh28 and tested for their responses to wsmv, mdmv, and sc ...	2011	21667271
nutrient budgets (c, n and p) and trophic dynamics of a brazilian tropical estuary: barra das jangadas.	this paper focuses on the nutrient dynamics of a tropical estuary on the northeastern brazilian coast, studied using the loicz biogeochemical budgeting protocol. we describe the methodology and assumptions underlying this model. input data (monthly for rainfall, evaporation, river discharge, and concentrations of salt, phosphorus and nitrogen) were obtained during field campaigns in the barra das jangadas estuary (bje) over a 5 years period (1999 to 2003). mass balance results indicate large inp ...	2011	21670871
bacillus rhizosphaerae sp. nov., an novel diazotrophic bacterium isolated from sugarcane rhizosphere soil.	a gram-positive, non-pigmented, rod-shaped, diazotrophic bacterial strain, designated sc-n012(t), was isolated from rhizosphere soil of sugarcane and was subjected to a polyphasic taxonomic study. the strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus bacillus. sequence analysis of the 16s rrna gene of sc-n012(t) revealed the closest match (98.9% pair wise similarity) with bacillus clausii dsm 8716(t). however, dna- ...	2011	21671194
differential gene expression in sugarcane in response to challenge by fungal pathogen ustilago scitaminea revealed by cdna-aflp.	differential gene expression in sugarcane during sugarcane-ustilago scitaminea interaction was conducted in a smut-resistant genotype. using cdna-aflp along with silver staining, a total of 136 transcript-derived fragments (tdfs) were found to be differentially expressed in response to challenge by u. scitaminea. forty tdfs, 34 newly induced plus six with obvious upregulated expression after infection, were sequenced and validated by rt-pcr analysis. these results demonstrated that the expressio ...	2011	21792273
sugarcane glycoproteins may act as signals for the production of xanthan in the plant-associated bacterium xanthomonas albilineans.	visual symptoms of leaf scald necrosis in sugarcane (saccharum officinarum) leaves develop in parallel to the accumulation of a fibrous material invading exocellular spaces and both xylem and phloem. these fibers are produced and secreted by the plant-associated bacterium xanthomonas albilineans. electron microscopy and specific staining methods for polysaccharides reveal the polysaccharidic nature of this material. these polysaccharides are not present in healthy leaves or in those from disease ...	2011	21791980
chronic consumption of distilled sugarcane spirit induces anxiolytic-like effects in mice.	chronic ethanol consumption is a major public health problem throughout the world. we investigated the anxiolytic-like effects and the possible ever injury induced by the chronic consumption of ethanol or sugarcane spirit in mice.	2011	21789394
development of a low-cost sterilization biological indicator using bacillus atrophaeus by solid-state fermentation.	the production of biological indicators involving bacterial sporulation and multi-step downstream processes has been described. the goal of the present work was to use fermented material as the final product in a biological indicator, thereby reducing processing steps and costs. the performance of three different inexpensive supports (vermiculite, sand, and sugarcane bagasse) was assessed by determining bacillus atrophaeus sporulation during solid-state fermentation and by assessing the direct u ...	2011	21785930
lipid production from yarrowia lipolytica po1g grown in sugarcane bagasse hydrolysate.	this study investigated the possibility of utilizing detoxified sugarcane bagasse hydrolysate (dscbh) as an alternative carbon source to culture yarrowia lipolytica po1g for microbial oil and biodiesel production. sugarcane bagasse hydrolysis with 2.5% hcl resulted in maximum total sugar concentration (21.38g/l) in which 13.59g/l is xylose, 3.98g/l is glucose, and 2.78g/l is arabinose. detoxification of scbh by ca(oh)(2) neutralization reduced the concentration of 5-hydroxymethylfurfural and fur ...	2011	21757339
poly-lactic acid synthesis for application in biomedical devices - a review.	bioabsorbable polymers are considered a suitable alternative to the improvement and development of numerous applications in medicine. poly-lactic acid (pla,) is one of the most promising biopolymers due to the fact that the monomers may produced from non toxic renewable feedstock as well as is naturally occurring organic acid. lactic acid can be made by fermentation of sugars obtained from renewable resources as such sugarcane. therefore, pla is an eco-friendly product with better features for u ...	2011	21756992
leviserpin: a serine peptidase inhibitor (serpin) from the sugarcane weevil sphenophorus levis.	serine peptidase inhibitors (serpins) form a superfamily of proteins covering abroad spectrum of different biological functions. here we describe the inhibitory characterization of leviserpin, the first serpin from the sugar cane weevil sphenophorus levis. leviserpin was able to inhibit bovine trypsin by the formation of the covalent complex serpin-peptidase, demonstrated by sds-page and mass spectroscopy analysis. we also have determined the cleavage site at the reactive center loop, by the ana ...	2011	21748377
production of bioethanol, methane and heat from sugarcane bagasse in a biorefinery concept.	the potential of biogas production from the residues of second generation bioethanol production was investigated taking into consideration two types of pretreatment: lime or alkaline hydrogen peroxide. bagasse was pretreated, enzymatically hydrolyzed and the wastes from pretreatment and hydrolysis were used to produce biogas. results have shown that if pretreatment is carried out at a bagasse concentration of 4% dm, the highest global methane production is obtained with the peroxide pretreatment ...	2011	21689929
high molecular variability of sugarcane bacilliform viruses in guadeloupe implying the existence of at least three new species.	thirty-five unique partial sugarcane bacilliform virus (scbv) sequences extending over 529bp were identified in sugarcane samples from guadeloupe diagnosed by immunocapture-pcr (ic-pcr) using specific badnavirus primers. phylogenetic analysis of these sequences along with the two known genome sequences of sugarcane bacilliform mor virus (scbmv) and sugarcane bacilliform im virus (scbimv) revealed high molecular variability in the scbv genome. seven phylogenetic groups, named a to g, were charact ...	2011	21741419
effects of d-003 (10 mg/day) on bone mineral density of the lumbar spine and femoral neck in postmenopausal women: a randomized, double-blinded study.	increased osteoclast activity is a pivotal finding in osteoporosis. this increase is mediated via the mevalonate-to-cholesterol pathway, which is involved in producing the intermediates required for osteoclast activity. d-003, a mixture of high molecular weight sugarcane wax acids, has been shown to inhibit cholesterol synthesis prior to mevalonate production, resulting in a reduction of bone loss and resorption in ovariectomized rats. moreover, previous studies have demonstrated that short-term ...	2011	21716593
plasmodium falciparum and helminth coinfections among schoolchildren in relation to agro-ecosystems in mvomero district, tanzania.	in sub-saharan africa, some individuals infected with malaria are also infected with helminths. however, the magnitude and distribution of such coinfections in relation to eco-systems remains poorly defined. this study was undertaken to determine the prevalence of plasmodium falciparum and helminth coinfections among schoolchildren in relation to agro-ecosystems in mvomero district, tanzania.	2011	21741929
production and characterization of a glycolipid biosurfactant, mannosylerythritol lipid b, from sugarcane juice by ustilago scitaminea nbrc 32730.	mannosylerythritol lipids (mels) are glycolipid biosurfactants excreted by fungal strains. they show not only excellent surface-active properties but also versatile biochemical actions. ustilago scitaminea nbrc 32730 has been reported mainly to produce a mono-acetylated and di-acylated mel, mel-b, from sucrose as sole carbon source. in order to make biosurfactant production more efficient, we focused our attention on the use of sugarcane juice, one of the most economical resources. the fungal st ...	2011	21737925
complete genomic sequence analysis of a highly virulent isolate revealed a novel strain of sugarcane mosaic virus.	sugarcane mosaic virus (scmv) is the most prevalent virus causing maize dwarf mosaic disease in northern china. a scmv isolate, bd8, was obtained from the maize showing dwarf and mosaic symptoms in baoding, china. the complete genomic sequence of bd8 is 9,576 nucleotides (nt) excluding the poly(a) tail. it contains one single open reading frame of 9,192-ánt and encodes a large polyprotein of 3,063-áamino acids (aa), flanked by a 5'-untranslated region (utr) of 148-ánt and a 3'-utr of 236-ánt. th ...	2011	21833715
differential protein expression in sugarcane during sugarcane-sporisorium scitamineum interaction revealed by 2-de and maldi-tof-tof/ms.	to understand the molecular basis of a specific plant-pathogen interaction, it is important to identify plant proteins that respond to the pathogen attack. two sugarcane varieties, nco376 and ya71-374, were used in this study. by applying 2-dimensional electrophoresis (2-de), the protein expression profile of sugarcane after inoculating with sporisorium scitamineum was analyzed. in total, 23 differentially expressed proteins were identified by maldi-tof-tof/ms. bioinformatics analysis revealed t ...	2011	21822403
scientific challenges of bioethanol production in brazil.	bioethanol (fuel alcohol) has been produced by industrial alcoholic fermentation processes in brazil since the beginning of the twentieth century. currently, 432 mills and distilleries crush about 625 million tons of sugarcane per crop, producing about 27 billion liters of ethanol and 38.7 million tons of sugar. the production of bioethanol from sugarcane represents a major large-scale technology capable of producing biofuel efficiently and economically, providing viable substitutes to gasoline. ...	2011	21735264
nutritional value of sugarcane silage enriched with corn grain, urea, and minerals as feed supplement on growth performance of beef steers grazing stargrass.	the objectives were to evaluate the silage quality of sugarcane silage enriched (as fed) with corn grain ground (10%), urea (1.5%), and mineral premix (0.5%) and its effects as a feed supplement on growth performance of beef steers grazing stargrass. firstly, in micro-silages, whole sugarcane enriched with corn grain, urea, and minerals (wsce) were ensilaged by 0, 20, 40, and 60 days. crude protein (cp) and lactic acid (la) increased linearly (p < 0.05) and true protein decreased linearly (p < 0 ...	2011	20711753
genetic transformation with untranslatable coat protein gene of sugarcane yellow leaf virus reduces virus titers in sugarcane.	sugarcane yellow leaf syndrome, characterized by a yellowing of the leaf midrib followed by leaf necrosis and growth suppression, is caused by sugarcane yellow leaf virus (scylv). we produced scylv-resistant transgenic sugarcane from a susceptible cultivar (h62-4671) and determined the amount of virus present following inoculation. the transgenic plants were produced through biolistic bombardment of cell cultures with an untranslatable coat protein gene. presence of the transgene in regenerated ...	2011	20661641
biological detoxification of different hemicellulosic hydrolysates using issatchenkia occidentalis cctcc m 206097 yeast.	this work had as its main objective to contribute to the development of a biological detoxification of hemicellulose hydrolysates obtained from different biomass plants using issatchenkia occidentalis cctcc m 206097 yeast. tests with hemicellulosic hydrolysate of sugarcane bagasse in different concentrations were carried out to evaluate the influence of the hydrolysate concentration on the inhibitory compounds removal from the sugarcane bagasse hydrolysate, without reduction of sugar concentrati ...	2011	20844925
structural characteristics and phylogenetic analysis of the mitochondrial genome of the sugarcane borer, diatraea saccharalis (lepidoptera: crambidae).	the sugarcane borer, diatraea saccharalis (f.), is an economically important pest of several major crops in north and south america. there is great concern over potential resistance development to the valuable transgenic bacillus thuringiensis corn. cost-effective monitoring methods, especially regarding molecular markers that could detect early changes in resistance allele frequency in field populations of d. saccharalis, are needed. this article reports the complete mitochondrial genome sequen ...	2011	20849253
pretreatment of synthetic dairy wastewater using the sophorolipid-producing yeast candida bombicola.	the presence of high strength fats and oils in dairy industry wastewaters poses serious challenges for biological treatment systems, and, therefore, its pretreatment is necessary in order to remove them. in the present study, synthetic dairy wastewater prepared in the laboratory was pretreated using the sophorolipid-producing yeast candida bombicola in a laboratory-scale bioreactor under batch, fed-batch, and continuous modes of operation. to support the yeast growth, the wastewater was suppleme ...	2011	20821070
production of cellulases and xylanases under catabolic repression conditions from mutant pr-22 of cellulomonas flavigena.	derepressed mutant pr-22 was obtained by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) mutagenic treatment of cellulomonas flavigena pn-120. this mutant improved its xylanolytic activity from 26.9 to 40 u mg(-1) and cellulolytic activity from 1.9 to 4 u mg(-1); this represented rates almost 2 and 1.5 times higher, respectively, compared to its parent strain growing in sugarcane bagasse. either glucose or cellobiose was added to cultures of c. flavigena pn-120 and mutant pr-22 induced with sugarcan ...	2011	20803244
kinetics of lime pretreatment of sugarcane bagasse to enhance enzymatic hydrolysis.	the objective of this work was to determine the optimum conditions of sugarcane bagasse pretreatment with lime to increase the enzymatic hydrolysis of the polysaccharide component and to study the delignification kinetics. the first stage was an evaluation of the influence of temperature, reaction time, and lime concentration in the pretreatment performance measured as glucose release after hydrolysis using a 2(3) central composite design and response surface methodology. the maximum glucose yie ...	2011	20803263
transgenic expression of trehalulose synthase results in high concentrations of the sucrose isomer trehalulose in mature stems of field-grown sugarcane.	sugarcane plants were developed that produce the sucrose isomers trehalulose and isomaltulose through expression of a vacuole-targeted trehalulose synthase modified from the gene in 'pseudomonas mesoacidophila mx-45' and controlled by the maize ubiquitin (ubi-1) promoter. trehalulose concentration in juice increased with internode maturity, reaching about 600 mm, with near-complete conversion of sucrose in the most mature internodes. plants remained vigorous, and trehalulose production in select ...	2011	20492546
choice of macroinvertebrate metrics to evaluate stream conditions in atlantic forest, brazil.	the development of biomonitoring programs based on the macroinvertebrate community requires the understanding of species distribution patterns, as well as of the responses of the community to anthropogenic stressors. in this study, 49 metrics were tested as potential means of assessing the condition of 29 first- and second-order streams located in areas of differing types of land use in são paulo state, brazil. of the sampled streams, 15 were in well-preserved regions in the atlantic forest, 5 w ...	2011	20461547
factors associated with the suppressiveness of sugarcane soils to plant-parasitic nematodes.	observations in three australian sugarcane fields suggested that the soil just under the trash blanket (the covering of crop residue that remains on the soil surface after crops are harvested) was suppressive to plant-parasitic nematodes. roots were concentrated in this upper layer of soil but plant-parasitic nematode populations were relatively low and roots showed few signs of nematode damage. root biomass was much lower 15 cm further down the soil profile, where root health was poor and popul ...	2011	23431051
heterologous expression of manganese peroxidase in aspergillus niger and its effect on phenanthrene removal from soil.	a strain of aspergillus niger, previously isolated from sugarcane bagasse because of its capacity to degrade phenanthrene in soil by solid culture, was used to express a manganese peroxidase gene (mnp1) from phanerochaete chrysosporium, aiming at increasing its polycyclic aromatic hydrocarbons degradation capacity. transformants were selected based on their resistance to hygromycin b and the discoloration induced on poly r-478 dye by the peroxidase activity. the recombinant a. niger sbc2-t3 stra ...	2011	22286039
parasitism by the endoparasitoid, cotesia flavipes induces cellular immunosuppression and enhances susceptibility of the sugar cane borer, diatraea saccharalis to bacillus thuringiensis.	cotesia flavipes cameron (hymenoptera: braconidae), is a gregarious larval endoparasitoid of the sugarcane borer, diatraea saccharalis fabricius (lepidoptera: crambidae). the aim of this research was to analyze cellular immunosuppression of d. saccharalis parasitized by c. flavipes in terms of encapsulation, melanization, and hemocyte nodule formation. the encapsulation assay was done 1 and 6 days after parasitoid oviposition. in addition, the susceptibility of parasitized and nonparasitzed larv ...	2011	22225507
mating populations of fusarium section liseola from rice, sugarcane and maize.	mating studies were conducted on 78 isolates of fusarium species section liseola from rice, sugarcane and maize. from the crosses with tester strains of gibberella fujikuroi species complex, 64.1% (50 out of 78 isolates) were cross-fertile with tester strains of mating populations a to e. the results of the mating studies showed that of the 50 isolates, 19 belonged to mating population a (gibberella moniliformis), 18 to mating population b (gibberella sacchari), 4 to mating population e (gibbere ...	2011	24575220
expression and characterization of a gh39 β-xylosidase ii from caulobacter crescentus.	in the present work, the gene xynb2, encoding a β-xylosidase ii of the glycoside hydrolase 39 (gh39) family, of caulobacter crescentus was cloned and successfully overexpressed in escherichia coli dh10b. the recombinant protein (ccxynb2) was purified using nickel-sepharose affinity chromatography, with a recovery yield of 75.5 %. ccxynb2 appeared as a single band of 60 kda on a sodium dodecyl sulfate polyacrylamide gel and was recognized by a specific polyclonal antiserum. the predicted ccxynb2 ...	2012	23054825
metabolic engineering of klebsiella oxytoca m5a1 to produce optically pure d-lactate in mineral salts medium.	klebsiella oxytoca strains were constructed to produce optical pure d-lactate by ph-controlled batch fermentation in mineral salts medium. the alcohol dehydrogenase gene, adhe, and the phospho-transacetylase/acetate kinase a genes, pta-acka, were deleted from the wild type. kms002 (δadhe) and kms004 (δadhe δpta-acka) exhibited d-lactate production as a primary pathway for the regeneration of nad(+). both strains produced 11-13 g/l of d-lactate in medium containing 2% (w/v) glucose with yields of ...	2012	22728200
a novel dirigent protein gene with highly stem-specific expression from sugarcane, response to drought, salt and oxidative stresses.	dirigent and dirigent-like family proteins contain a number of proteins involved in lignification or in the response to pathogen infection and abiotic stress in plants. in the present study, a full-length cdna sequence of a dirigent-like gene designated scdir (genbank accession number jq622282) was obtained from sugarcane based on the stem full-length cdna library. the scdir gene was 819-bp long, including a 564-bp orf encoding 187 amino acid residues. the protein n-terminus contained signal pep ...	2012	22696141
production and characterization of violacein by locally isolated chromobacterium violaceum grown in agricultural wastes.	the present work highlighted the production of violacein by the locally isolated chromobacterium violaceum (genbank accession no. hm132057) in various agricultural waste materials (sugarcane bagasse, solid pineapple waste, molasses, brown sugar), as an alternative to the conventional rich medium. the highest yield for pigment production (0.82 g l⁻¹) was obtained using free cells when grown in 3 g of sugarcane bagasse supplemented with 10% (v/v) of l-tryptophan. a much lower yield (0.15 g l⁻¹) wa ...	2012	22278051
reuteran and levan as carbohydrate sinks in transgenic sugarcane.	the present study reports the effect of high molecular weight bacterial fructan (levan) and glucan (reuteran) on growth and carbohydrate partitioning in transgenic sugarcane plants. these biopolymers are products of bacterial glycosyltransferases, enzymes that catalyze the polymerization of glucose or fructose residues from sucrose. constructs, targeted to different subcellular compartments (cell wall and cytosol) and driven by the cauliflower mosaic virus-35s: maize-ubiquitin promoter, were int ...	2012	22903192
recovery of purified lactonic sophorolipids by spontaneous crystallization during the fermentation of sugarcane molasses with candida albicans o-13-1.	numerous studies have focused on how to obtain high yield of sophorolipids using low-cost materials as substrates, and there has been various work on the experimental methods for purifying lactonic sophorolipids. these studies have not yet obtained satisfied results in combining a low-cost fermentation process and the purification of lactonic sophorolipids. this study establishes a fed-batch fermentation process of purifying sophorolipids from candida albicans o-13-1 using low-cost sugarcane mol ...	2012	23040390
diversity and physiological characterization of d-xylose-fermenting yeasts isolated from the brazilian amazonian forest.	this study is the first to investigate the brazilian amazonian forest to identify new d-xylose-fermenting yeasts that might potentially be used in the production of ethanol from sugarcane bagasse hemicellulosic hydrolysates.	2012	22912807
decolorization of a recalcitrant organic compound (melanoidin) by a novel thermotolerant yeast, candida tropicalis rg-9.	sugarcane distilleries use molasses for ethanol production and generate large volume of effluent containing high biological oxygen demand (bod) and chemical oxygen demand (cod) along with melanoidin pigment. melanoidin is a recalcitrant compound that causes several toxic effects on living system, therefore, may be treated before disposal. the aim of this study was to isolate a potential thermotolerant melanoidin decolorizing yeast from natural resources, and optimized different physico-chemical ...	2012	22708874
evaluation of corn hybrids expressing cry1f, cry1a.105, cry2ab2, cry34ab1/cry35ab1, and cry3bb1 against southern united states insect pests.	studies were conducted across the southern united states to characterize the efficacy of multiple bacillus thuringiensis (bt) events in a field corn, zea mays l., hybrid for control of common lepidopteran and coleopteran pests. cry1f protein in event tc1507 and cry1a.105 + cry2ab2 proteins in event mon 89034 were evaluated against pests infesting corn on above-ground plant tissue including foliage, stalks, and ears. cry34ab1/cry35ab1 proteins in event das-59122-7 and cry3bb1 in event mon 88017 w ...	2012	23156183
beauveria brongniartii on white grubs attacking sugarcane in south africa.	beauveria brongniartii (saccardo) petch fungal infections were observed on the melolonthid hypopholis sommeri burmeister (coleoptera: scarabaeidae) at two sites (harden heights and canema) in the sugarcane producing area of the northern kwazulu-natal midlands of south africa. to initially identify the disease-causing fungus, 17 different fluorescently-labelled microsatellite pcr primers were used to target 78 isolates of beauveria spp. dna. microsatellite data resolved two distinct clusters of b ...	2012	22982079
biodegradation of s-triazine herbicide atrazine by enterobacter cloacae and burkholderia cepacia sp. from long-term treated sugarcane-cultivated soils in kenya.	in this study soils from sugarcane-cultivated fields were screened for bacterial species capable of atrazine (6-chloro-n²-ethyl-n⁴-isopropyl-1,3,5-triazine-2,4-diamine) degradation due to long exposure of the soils to this herbicide. to enrich for atrazine degraders, minimal salt medium containing atrazine as the sole n source and glucose as the c source was inoculated with soils impacted with this herbicide and incubated. bacterial growth was monitored by measuring optical density. the degradat ...	2012	22575004
beneficial effects of bacillus subtilis subsp. subtilis mori2, a honey-associated strain, on honeybee colony performance.	a bacillus spp. strain isolated from a honey sample in morillos (salta, argentina) was phylogenetically characterized as b. subtilis subsp. subtilis mori2. the strain was administered to bee colonies as a monoculture in one litre of sugarcane syrup (125 g/l) at a final concentration of 10(5) spores/ml to evaluate the bee colony performance. the treated colony was monitored, and any changes were compared with the control hives. all conditions were identical (weather, nourishment and supervision), ...	2012	26781735
production of cellulases from aspergillus niger ns-2 in solid state fermentation on agricultural and kitchen waste residues.	various agricultural and kitchen waste residues were assessed for their ability to support the production of a complete cellulase system by aspergillus niger ns-2 in solid state fermentation. untreated as well as acid and base-pretreated substrates including corn cobs, carrot peelings, composite, grass, leaves, orange peelings, pineapple peelings, potato peelings, rice husk, sugarcane bagasse, saw dust, wheat bran, wheat straw, simply moistened with water, were found to be well suited for the or ...	2012	22503148
sequential solid-state and submerged cultivation of aspergillus niger on sugarcane bagasse for the production of cellulase.	sequential solid-state and submerged cultivation with sugarcane bagasse as substrate for cellulase production by aspergillus niger a12 was assessed by measuring endoglucanase activity. an unconventional pre-culture with an initial fungal growth phase under solid-state cultivation was followed by a transition to submerged fermentation by adding the liquid culture medium to the mycelium grown on solid substrate. for comparison, control experiments were conducted using conventional submerged cultiv ...	2012	22409979
single cell oil of oleaginous fungi from the tropical mangrove wetlands as a potential feedstock for biodiesel.	single cell oils (scos) accumulated by oleaginous fungi have emerged as a potential alternative feedstock for biodiesel production. though fungi from mangrove ecosystem have been reported for production of several lignocellulolytic enzymes, they remain unexplored for their sco producing ability. thus, these oleaginous fungi from the mangrove ecosystem could be suitable candidates for production of scos from lignocellulosic biomass. the accumulation of lipids being species specific, strain select ...	2012	22646719
relations between phenotypic changes of spores and biofilm production by bacillus atrophaeus atcc 9372 growing in solid-state fermentation.	bacillus spp. spores are usually obtained from strains cultivated in artificial media. however, in natural habitats, spores are predominantly formed from bacteria present in highly surface-associated communities of cells. solid-state fermentation (ssf) is the culture method that best mimetizes the natural environment of many microorganisms that grow attached to the surface of solid particles. this study aims to confirm that sporulation through ssf of bacillus atrophaeus occurs by biofilm formati ...	2012	22526268
possible involvement of maize rop1 in the defence responses of plants to viral infection.	the expression of host genes can be altered during the process of viral infection. to investigate the viral infection-induced up-regulated gene expression changes of maize at different time intervals post-inoculation with sugarcane mosaic virus (scmv), a suppression subtractive hybridization cdna library was constructed. a total of 454 cdna clones were identified to be viral infection-induced up-regulated genes. the influence of rop1 on the infection of maize by scmv was investigated. the result ...	2012	22332840
genomic comparison of the endophyte herbaspirillum seropedicae smr1 and the phytopathogen herbaspirillum rubrisubalbicans m1 by suppressive subtractive hybridization and partial genome sequencing.	herbaspirillum rubrisubalbicans m1 causes the mottled stripe disease in sugarcane cv. b-4362. inoculation of this cultivar with herbaspirillum seropedicae smr1 does not produce disease symptoms. a comparison of the genomic sequences of these closely related species may permit a better understanding of contrasting phenotype such as endophytic association and pathogenic life style. to achieve this goal, we constructed suppressive subtractive hybridization (ssh) libraries to identify dna fragments ...	2012	22268687
identification and validation of reference genes to study the gene expression in gluconacetobacter diazotrophicus grown in different carbon sources using rt-qpcr.	gluconacetobacter diazotrophicus strain pal5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. currently, g. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens. reverse transcription fol ...	2012	22814372
identification of n-acyl homoserine lactones produced by gluconacetobacter diazotrophicus pal5 cultured in complex and synthetic media.	the endophytic diazotrophic gluconacetobacter diazotrophicus pal5 was originally isolated from sugarcane (saccharum officinarum). the biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. the recent genomic sequence of pal5 unveiled the presence of a quorum sensing (qs) system. qs are regulatory mechanisms that, through the production of signal molecules or autoinduce ...	2012	22350020
high-temperature ethanol fermentation by immobilized coculture of kluyveromyces marxianus and saccharomyces cerevisiae.	suspended and immobilized cocultures of the thermotolerant yeast, kluyveromyces marxianus dmku 3-1042 and the mesophilic flocculent yeast, saccharomyces cerevisiae m30 were studied for their abilities to improve production and stability of ethanol fermentation. sugarcane juice and blackstrap molasses, at initial sugar concentrations of 220 g/l, were used as carbon sources. the results indicated that the coculture system could improve ethanol production from both sugarcane juice and blackstrap mo ...	2012	22608995
the influence of presaccharification, fermentation temperature and yeast strain on ethanol production from sugarcane bagasse.	ethanol can be produced from cellulosic biomass in a process known as simultaneous saccharification and fermentation (ssf). the presence of yeast together with the cellulolytic enzyme complex reduces the accumulation of sugars within the reactor, increasing the ethanol yield and saccharification rate. this paper reports the isolation of saccharomyces cerevisiae lbm-1, a strain capable of growth at 42 °c. in addition, s. cerevisiae lbm-1 and kluyveromyces marxianus ufv-3 were able to ferment suga ...	2012	22285296
lactobacillus reuteri crl 1101 highly produces mannitol from sugarcane molasses as carbon source.	mannitol is a natural polyol extensively used in the food industry as low-calorie sugar being applicable for diabetic food products. we aimed to evaluate mannitol production by lactobacillus reuteri crl 1101 using sugarcane molasses as low-cost energy source. mannitol formation was studied in free-ph batch cultures using 3-10% (w/v) molasses concentrations at 37 °c and 30 °c under static and agitated conditions during 48 h. l. reuteri crl 1101 grew well in all assayed media and heterofermentativ ...	2012	22350320
hantavirus pulmonary syndrome in santa cruz, bolivia: outbreak investigation and antibody prevalence study.	we report the results of an investigation of a small outbreak of hantavirus pulmonary syndrome in 2002 in the department of santa cruz, bolivia, where the disease had not previously been reported. two cases were initially reported. the first case was a physician infected with laguna negra virus during a weekend visit to his ranch. four other persons living on the ranch were igm antibody-positive, two of whom were symptomatic for mild hantavirus pulmonary syndrome. the second case was a migrant s ...	2012	23094116
genotypic and phenotypic diversity of pgpr fluorescent pseudomonads isolated from the rhizosphere of sugarcane (saccharum officinarum l.).	the genetic diversity of plant growth-promoting rhizobacterial (pgpr) fluorescent pseudomonads associated with the sugarcane (saccharum officinarum l.) rhizosphere was analyzed. selected isolates were screened for plant growthpromoting properties including production of indole acetic acid, phosphate solubilization, denitrification ability, and production of antifungal metabolites. furthermore, 16s rdna sequence analysis was performed to identify and differentiate these isolates. based on 16s rdn ...	2012	22297215
metabolic engineering of saccharomyces cerevisiae for increased bioconversion of lignocellulose to ethanol.	the absence of pentose-utilizing enzymes in saccharomyces cerevisiae is an obstacle for efficiently converting lignocellulosic materials to ethanol. in the present study, the genes coding xylose reductase (xyl1) and xylitol dehydrogenase (xyl2) from pichia stipitis were successfully engineered into s. cerevisae. as compared to the control transformant, engineering of xyl1 and xyl2 into yeasts significantly increased the microbial biomass (8.1 vs. 3.4 g/l), xylose consumption rate (0.15 vs. 0.02 ...	2012	23997337
stimulation of extracellular invertase production from spent yeast when sugarcane pressmud used as substrate through solid state fermentation.	efforts were made to utilize the waste/by-product of two agro-process industries namely pressmud from sugar processing industries and spent yeast from distilleries manufacturing ethanol from cane molasses, for the production of microbial invertase. our experimentation indicated that these two sources could be ideally utilized for the production of invertase through solid substrate fermentation (ssf). ssf with spent yeast had given highest specific activity of 430 u/mg in 72 h of fermentation. in ...	2012	23420549
genetic characterization and construction of an auxotrophic strain of saccharomyces cerevisiae jp1, a brazilian industrial yeast strain for bioethanol production.	used for millennia to produce beverages and food, saccharomyces cerevisiae also became a workhorse in the production of biofuels, most notably bioethanol. yeast strains have acquired distinct characteristics that are the result of evolutionary adaptation to the stresses of industrial ethanol production. jp1 is a dominant industrial s. cerevisiae strain isolated from a sugarcane mill and is becoming increasingly popular for bioethanol production in brazil. in this work, we carried out the genetic ...	2012	22892884
acid black 48 dye biosorption using saccharomyces cerevisiae immobilized with treated sugarcane bagasse.	the textile industry consumes large quantities of water and chemicals, especially in dyeing and finishing processes. textile dye adsorption can be accomplished with natural or synthetic compounds. cell immobilization using biomaterials allows the reduction of toxicity and mechanical resistance and opens spaces within the matrix for cell growth. the use of natural materials, such as sugarcane bagasse, is promising due to the low costs involved. the aim of the present study was to evaluate the use ...	2012	22864427
the consequences of lactobacillus vini and dekkera bruxellensis as contaminants of the sugarcane-based ethanol fermentation.	this work describes the effects of the presence of the yeast dekkera bruxellensis and the bacterium lactobacillus vini on the industrial production of ethanol from sugarcane fermentation. both contaminants were quantified in industrial samples, and their presence was correlated to a decrease in ethanol concentration and accumulation of sugar. then, laboratory mixed-cell fermentations were carried out to evaluate the effects of these presumed contaminants on the viability of saccharomyces cerevis ...	2012	22842986
identification of amino acids important for substrate specificity in sucrose transporters using gene shuffling.	plant sucrose transporters (suts) are h(+)-coupled uptake transporters. type i and ii (suts) are phylogenetically related but have different substrate specificities. type i suts transport sucrose, maltose, and a wide range of natural and synthetic α- and β-glucosides. type ii suts are more selective for sucrose and maltose. here, we investigated the structural basis for this difference in substrate specificity. we used a novel gene shuffling method called synthetic template shuffling to introduc ...	2012	22807445
metschnikowia saccharicola sp. nov. and metschnikowia lopburiensis sp. nov., two novel yeast species isolated from phylloplane in thailand.	five strains representing two novel anamorphic yeast species were isolated from the external surface of sugarcane leaves (dmku-rk16, dmku-rk24, dmku-rk198 and dmku-rk500(t)) and rice leaf (dmku-rk277(t)) by an enrichment technique. on the basis of morphological, biochemical, physiological and chemotaxonomic characteristics, the sequence analysis of the d1/d2 domain of the large subunit (lsu) rrna gene and the internal transcribed spacer region, the five strains were assigned to be two novel spec ...	2012	22791018
study of sugarcane pieces as yeast supports for ethanol production from sugarcane juice and molasses using newly isolated yeast from toddy sap.	a repeated batch fermentation system was used to produce ethanol using saccharomyces cerevisiae strain (ncim 3640) immobilized on sugarcane (saccharum officinarum l.) pieces. for comparison free cells were also used to produce ethanol by repeated batch fermentation. scanning electron microscopy evidently showed that cell immobilization resulted in firm adsorption of the yeast cells within subsurface cavities, capillary flow through the vessels of the vascular bundle structure, and attachment of ...	2012	22783132
pretreatment of sugarcane bagasse with nh4oh-h2o2 and ionic liquid for efficient hydrolysis and bioethanol production.	an efficient pretreatment method using nh(4)oh-h(2)o(2) and ionic liquid (il) was developed for the recovery of cellulose from sugarcane bagasse (scb). the regenerated scb from the combined pretreatment exhibited significantly enhanced enzymatic digestibility with an efficiency of 91.4% after 12h of hydrolysis, which was 64% higher than the efficiency observed for the regenerated scb after the individual nh(4)oh-h(2)o(2) pretreatment. 1-allyl-3-methylimidazolium chloride ([amim]cl) dissolved the ...	2012	22728201
whole-genome sequencing of the efficient industrial fuel-ethanol fermentative saccharomyces cerevisiae strain cat-1.	the saccharomyces cerevisiae strains widely used for industrial fuel-ethanol production have been developed by selection, but their underlying beneficial genetic polymorphisms remain unknown. here, we report the draft whole-genome sequence of the s. cerevisiae strain cat-1, which is a dominant fuel-ethanol fermentative strain from the sugarcane industry in brazil. our results indicate that strain cat-1 is a highly heterozygous diploid yeast strain, and the ~12-mb genome of cat-1, when compared w ...	2012	22562254
characterization of laccase isoforms produced by pleurotus ostreatus in solid state fermentation of sugarcane bagasse.	laccases are oxidative enzymes linked to biological degradation of lignin. the aim of this work was to evaluate the effect of inducers and different concentrations of nitrogen on production level of total laccase activity and pattern of laccase isoforms, produced in solid state fermentation of sugarcane bagasse by a selected strain of pleurotus ostreatus. the addition of yeast extract 5 g/l, copper sulfate 150 μm and ferulic acid 2 mm provided highest enzymatic activity (167 u/g) and zymograms i ...	2012	22487128
understanding physiological responses to pre-treatment inhibitors in ethanologenic fermentations.	alcohol-based liquid fuels feature significantly in the political and social agendas of many countries, seeking energy sustainability. it is certain that ethanol will be the entry point for many sustainable processes. conventional ethanol production using maize- and sugarcane-based carbohydrates with saccharomyces cerevisiae is well established, while lignocellulose-based processes are receiving growing interest despite posing greater technical and scientific challenges. a significant challenge ...	2012	22331581
production of succinic acid from sucrose and sugarcane molasses by metabolically engineered escherichia coli.	sucrose-utilizing genes (csckb and csca) from escherichia coli ko11 were cloned and expressed in a metabolically engineered e. coli kj122 to enhance succinate production from sucrose. kj122 harboring a recombinant plasmid, pkjsuc, was screened for the efficient sucrose utilization by growth-based selection and adaptation. kj122-pkjsuc-24t efficiently utilized sucrose in a low-cost medium to produce high succinate concentration with less accumulation of by-products. succinate concentrations of 51 ...	2012	22023966
Recombinant expression, localization and in vitro inhibition of midgut cysteine peptidase (Sl-CathL) from sugarcane weevil, Sphenophorus levis.	A cDNA coding for a digestive cathepsin L, denominated Sl-CathL, was isolated from a cDNA library of Sphenophorus levis larvae, representing the most abundant EST (10.49%) responsible for proteolysis in the midgut. The open reading frame of 972bp encodes a preproenzyme similar to midgut cathepsin L-like enzymes in other coleopterans. Recombinant Sl-CathL was expressed in Pichia pastoris, with molecular mass of about 42kDa. The recombinant protein was catalytically activated at low pH and the mat ...	2012	22100428
in vivo interactions of entomopathogenic fungi, beauveria spp. and metarhizium anisopliae with selected opportunistic soil fungi of sugarcane ecosystem.	in the present study, the interactions of entomopathogenic fungi viz., beauveria bassiana, beauveria brongniartii and metarhizium anisopliae among themselves and three other opportunistic soil fungi from the sugarcane ecosystem namely, fusarium saachari, aspergillus sp. and penecillium sp. were assayed in vivo against galleria mellonella larvae. the tested fungi were co-applied on iv instar g. mellonella @ 1 x 10(7) ml(-1), in combinations of two, at the interval of 24 hrs either preceding or su ...	2012	23359998
chrysoporthe cubensis: a new source of cellulases and hemicellulases to application in biomass saccharification processes.	the plant pathogenic fungus chrysoporthe cubensis was cultivated under solid state employing different substrates and the highest endoglucanase (33.84ug(-1)), fpase (2.52ug(-1)), β-glucosidase (21.55ug(-1)) and xylanase (362.38ug(-1)) activities were obtained using wheat bran as carbon source. cellulases and xylanase produced by c. cubensis showed maximal hydrolysis rate at ph 4.0 and in a temperature range of 50-60°c. all enzymatic activities were highly stable at 40 and 50°c through 48h of pre ...	2012	23313674
solvent extraction of antioxidants from steam exploded sugarcane bagasse and enzymatic convertibility of the solid fraction.	solvent extraction of steam exploded lignocellulosic biomass may be a potential way to obtain antioxidative extracts and to enhance the enzymatic convertibility of the solid residue. boiling solvent extraction (bse) showed higher solid and phenolic yields than room temperature extraction. solubilities of phenolics and sugars were higher in anhydrous ethanol (ae) and deionized water (dw) than in ethyl acetate under each individual extraction condition. the antioxidant activities of the ae and dw ...	2012	23280180
rnai-mediated abrogation of trehalase expression does not affect trehalase activity in sugarcane.	to engineer trehalose metabolism in sugarcane (saccharum spp. hybrids) two transgenes were introduced to the genome: trehalose-6-phosphate synthase- phosphatase (tpsp), to increase trehalose biosynthesis and an rnai transgene specific for trehalase, to abrogate trehalose catabolism. in rnai-expressing lines trehalase expression was abrogated in many plants however no decrease in trehalase activity was observed. in tpsp lines trehalase activity was significantly higher. no events of co-integratio ...	2012	23539210
[assessment of health-related quality of life of sugarcane cutters in the pre-harvest and harvest periods].	to assess health-related quality of life in sugarcane cutters.	2012	23503542
description of a unique, complex feeding socket caused by the putative primitive root-knot nematode, meloidogyne kikuyensis.	meloidogyne kikuyensis produces unique galls that form on one side of the root resembling nitrogen-fixing nodules that are produced on legumes in response to infection by rhizobium and related bacteria. the gall caused by this root-knot nematode is made up of a complex feeding socket composed of several giant cells that are ramified with xylem vessels extending perpendicular from the vascular cylinder. the anterior portion of the second-stage juvenile, which develops into an adult, plugs into th ...	2012	23482409
tailor-made fructan synthesis in plants: a review.	fructan, a fructose polymer, is produced by many bacteria and plants. fructan is used as carbohydrate reserve, and in bacteria also as protective outside layer. chicory is a commercial fructan producing crop. the disadvantage of this crop is its fructan breakdown before harvest. studies using genetically modification showed that fructan biosynthesis is difficult to steer in chicory. alternatives for production of tailor-made fructan, fructan with a desired polymer length and linkage type, are or ...	2012	23465900
Ethanol production by continuous fermentation of d-(+)-cellobiose, d-(+)-xylose and sugarcane bagasse hydrolysate using the thermoanaerobe Caloramator boliviensis.	The recently isolated anaerobic bacterium Caloramator boliviensis with an optimum growth temperature of 60°C can efficiently convert hexoses and pentoses into ethanol. When fermentations of pure sugars and a pentose-rich sugarcane bagasse hydrolysate were carried out in a packed bed reactor with immobilized cells of C. boliviensis, more than 98% of substrates were converted. Ethanol yields of 0.40-0.46g/g of sugar were obtained when sugarcane bagasse hydrolysate was fermented. These features rev ...	2012	22055102
Conceptual design of cost-effective and environmentally-friendly configurations for fuel ethanol production from sugarcane by knowledge-based process synthesis.	In this work, the hierarchical decomposition methodology was used to conceptually design the production of fuel ethanol from sugarcane. The decomposition of the process into six levels of analysis was carried out. Several options of technological configurations were assessed in each level considering economic and environmental criteria. The most promising alternatives were chosen rejecting the ones with a least favorable performance. Aspen Plus was employed for simulation of each one of the tech ...	2012	22137752
Cell recycling during repeated very high gravity bio-ethanol fermentations using the industrial Saccharomyces cerevisiae strain PE-2.	A very high gravity (VHG) repeated-batch fermentation system using an industrial strain of Saccharomyces cerevisiae PE-2 (isolated from sugarcane-to-ethanol distillery in Brazil) and mimicking industrially relevant conditions (high inoculation rates and low O(2) availability) was successfully operated during fifteen consecutive fermentation cycles, attaining ethanol at 17.1 ± 0.2% (v/v) with a batch productivity of 3.5 ± 0.04 g l(-1) h(-1). Moreover, this innovative operational strategy (biomass ...	2012	21898130
sugarcane genes differentially expressed in response to puccinia melanocephala infection: identification and transcript profiling.	brown rust caused by the fungus puccinia melanocephala is a major disease of sugarcane (saccharum spp.). a sugarcane mutant, obtained by chemical mutagenesis of the susceptible variety b4362, showed a post-haustorial hypersensitive response (hr)-mediated resistance to the pathogen and was used to identify genes differentially expressed in response to p. melanocephala via suppression subtractive hybridization (ssh). tester cdna was derived from the brown rust-resistant mutant after inoculation wi ...	2012	22212461
production of xylanase under solid-state fermentation by aspergillus tubingensis jp-1 and its application.	the production of extracellular xylanase by a locally isolated strain of aspergillus tubingensis jp-1 was studied under solid-state fermentation. among the various agro residues used wheat straw was found to be the best for high yield of xylanase with poor cellulase production. the influence of various parameters such as initial ph, moisture, moistening agents, nitrogen sources, additives, surfactants and pretreatment of substrates were investigated. the production of the xylanase reached a peak ...	2012	22271252
fiber fermentability effects on energy and macronutrient digestibility, fecal parameters, postprandial metabolite responses, and colon histology of overweight cats.	considering the different potential benefits of divergent fiber ingredients, the effect of 3 fiber sources on the energy and macronutrient digestibility, fermentation product formation, postprandial metabolite responses, and colon histology of overweight cats fed kibble diets was compared. twenty-four healthy adult cats were assigned in a complete randomized block design to 2 groups of 12 animals, and 3 animals from each group were fed 1 of 4 of the following kibble diets: control (co; 11.5% die ...	2012	22247109
genomic insights into strategies used by xanthomonas albilineans with its reduced artillery to spread within sugarcane xylem vessels.	xanthomonas albilineans causes leaf scald, a lethal disease of sugarcane. x. albilineans exhibits distinctive pathogenic mechanisms, ecology and taxonomy compared to other species of xanthomonas. for example, this species produces a potent dna gyrase inhibitor called albicidin that is largely responsible for inducing disease symptoms; its habitat is limited to xylem; and the species exhibits large variability. a first manuscript on the complete genome sequence of the highly pathogenic x. albilin ...	2012	23171051
the type iii secretion system is necessary for the development of a pathogenic and endophytic interaction between herbaspirillum rubrisubalbicans and poaceae.	herbaspirillum rubrisubalbicans was first identified as a bacterial plant pathogen, causing the mottled stripe disease in sugarcane. h. rubrisubalbicans can also associate with various plants of economic interest in a non pathogenic manner.	2012	22672506
the determination of assay for laccase of bacillus subtilis wpi with two classes of chemical compounds as substrates.	ligninolytic enzyme complexes are involved in lignin degradation. among them laccases are outstanding because they use molecular oxygen as a co-substrate instead of hydrogen peroxide as used by peroxidases. bacterial laccase of bacillus genus was first reported in claus and filip (microbiol res 152:209-216, 1997), since then more bacterial laccases have been found. in this research, laccase-producing bacteria were screened from pulp and paper industry wastewater, bagass and sugarcane rhizosphere ...	2012	24293734
plant growth-promoting nitrogen-fixing enterobacteria are in association with sugarcane plants growing in guangxi, china.	the current nitrogen fertilization for sugarcane production in guangxi, the major sugarcane-producing area in china, is very high. we aim to reduce nitrogen fertilization and improve sugarcane production in guangxi with the help of indigenous sugarcane-associated nitrogen-fixing bacteria. we initially obtained 196 fast-growing bacterial isolates associated with the main sugarcane cultivar roc22 plants in fields using a nitrogen-deficient minimal medium and screened out 43 nitrogen-fixing isolate ...	2012	22510648
ethanol fermentation of sugarcane molasses by zymomonas mobilis mtcc 92 immobilized in luffa cylindrica l. sponge discs and ca-alginate matrices.	bio-ethanol production from cane molasses (diluted to 15 % sugar w/v) was studied using the bacterium, zymomonas mobilis mtcc 92 entrapped in luffa (luffa cylindrica l.) sponge discs and ca-alginate gel beads as the immobilizing matrices. at the end of 96 h fermentation, the final ethanol concentrations were 58.7 ± 0.09 and 59.1 ± 0.08 g/l molasses with luffa and ca-alginate entrapped z. mobilis cells, respectively exhibiting 83.25 ± 0.03 and 84.6 ± 0.02 % sugar conversion. there was no statisti ...	2012	24031981
effect of acid hydrolysis and fungal biotreatment on agro-industrial wastes for obtainment of free sugars for bioethanol production.	this study was designed to evaluate selected chemical and microbiological treatments for the conversion of certain local agro-industrial wastes (rice straw, corn stalks, sawdust, sugar beet waste and sugarcane bagasse) to ethanol. the chemical composition of these feedstocks was determined. conversion of wastes to free sugars by acid hydrolysis varied from one treatment to another. in single-stage dilute acid hydrolysis, increasing acid concentration from 1 % (v/v) to 5 % (v/v) decreased the con ...	2012	24031984
larval habitat associations with human land uses, roads, rivers, and land cover for anopheles albimanus, a. pseudopunctipennis, and a. punctimacula (diptera: culicidae) in coastal and highland ecuador.	larval habitat for three highland anopheles species: anopheles albimanus wiedemann, anopheles pseudopunctipennis theobald, and anopheles punctimacula dyar and knab was related to human land uses, rivers, roads, and remotely sensed land cover classifications in the western ecuadorian andes. of the five commonly observed human land uses, cattle pasture (n = 30) provided potentially suitable habitat for a. punctimacula and a. albimanus in less than 14% of sites, and was related in a principal compo ...	2012	22454623
candidates for symbiotic control of sugarcane white leaf disease.	the leafhopper matsumuratettix hiroglyphicus (matsumura) is the most important vector of a phytoplasma pathogen causing sugarcane white leaf (scwl) disease. the purpose of this study was to evaluate candidate bacterial symbionts for possible use as vehicles in the control of the disease. 16s rrna bacterial genes were amplified from whole bodies of m. hiroglyphicus leafhoppers and analyzed by cloning and sequencing. two dominant groups were found: one belonged to the betaproteobacteria that did n ...	2012	22798373
sugarcane growth promotion by the endophytic bacterium pantoea agglomerans 33.1.	the promotion of sugarcane growth by the endophytic pantoea agglomerans strain 33.1 was studied under gnotobiotic and greenhouse conditions. the green fluorescent protein (gfp)-tagged strain p. agglomerans 33.1::pnkgfp was monitored in vitro in sugarcane plants by microscopy, reisolation, and quantitative pcr (qpcr). using qpcr and reisolation 4 and 15 days after inoculation, we observed that gfp-tagged strains reached similar density levels both in the rhizosphere and inside the roots and aeria ...	2012	22865062
epicoccum nigrum p16, a sugarcane endophyte, produces antifungal compounds and induces root growth.	sugarcane is one of the most important crops in brazil, mainly because of its use in biofuel production. recent studies have sought to determine the role of sugarcane endophytic microbial diversity in microorganism-plant interactions, and their biotechnological potential. epicoccum nigrum is an important sugarcane endophytic fungus that has been associated with the biological control of phytopathogens, and the production of secondary metabolites. in spite of several studies carried out to define ...	2012	22675473
utilization of sugarcane habitat by feral pig (sus scrofa) in northern tropical queensland: evidence from the stable isotope composition of hair.	feral pigs (sus scrofa) are an invasive species that disrupt ecosystem functioning throughout their introduced range. in tropical environments, feral pigs are associated with predation and displacement of endangered species, modification of habitat, and act as a vector for the spread of exotic vegetation and disease. across many parts of their introduced range, the diet of feral pigs is poorly known. although the remote location and difficult terrain of far north queensland makes observing feral ...	2012	22957029
selection of thermophilic and thermotolerant fungi for the production of cellulases and xylanases under solid-state fermentation.	twenty-seven thermophilic and thermotolerant fungal strains were isolated from soil, decaying organic matter and sugarcane piles based on their ability to grow at 45°c on medium containing corn straw and cardboard as carbon sources. these fungi were identified in the genera aspergillus, thermomyces, myceliophthora, thermomucor and candida. the majority of the isolated strains produced xylanase and cellulases under solid state fermentation (ssf). the highest cellulase and xylanase productions wer ...	2012	24031929
production of thermophilic endo-β-1,4-xylanases by aspergillus fumigatus fbspe-05 using agro-industrial by-products.	in the present paper, endo-β-1,4-xylanase production by aspergillus fumigatus was evaluated in solid-state fermentation using low-cost substrates such as sugarcane bagasse (scb), brewer's spent grain (bsg), and wheat bran (wb). the partial characterization of the crude enzyme was also performed. in the experimental conditions, the highest levels of endo-β-1,4-xylanase production by a. fumigatus fbspe-05 occurred within 8 days incubation when using scb/liquid medium at 1:2 ratio (219.5 u g(-1)) a ...	2012	22328248
genetically modified sugarcane for bioenergy generation.	sugarcane breeding has significantly progressed over the past 30 years, but attempts to further increase crop yield have been limited due to the complexity of the sugarcane genome. an alternative to boost the crop yield is the introduction of genes encoding desirable traits in the elite sugarcane cultivars. genetically modified sugarcane with increased yield and pest and disease resistance has already proven its value not only by the increased sugar content but also for the improvement of the cr ...	2012	22093808