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rapid discrimination of bergamot essential oil by paper spray mass spectrometry and chemometric analysis.a novel approach for the rapid discrimination of bergamot essential oil from other citrus fruits oils is presented. the method was developed using paper spray mass spectrometry (ps-ms) allowing for a rapid molecular profiling coupled with a statistic tool for a precise and reliable discrimination between the bergamot complex matrix and other similar matrices, commonly used for its reconstitution. ambient mass spectrometry possesses the ability to record mass spectra of ordinary samples, in their ...201627460885
aluminum toxicity-induced alterations of leaf proteome in two citrus species differing in aluminum tolerance.seedlings of aluminum-tolerant 'xuegan' (citrus sinensis) and al-intolerant 'sour pummelo' (citrus grandis) were fertigated for 18 weeks with nutrient solution containing 0 and 1.2 mm alcl₃·6h₂o. al toxicity-induced inhibition of photosynthesis and the decrease of total soluble protein only occurred in c. grandis leaves, demonstrating that c. sinensis had higher al tolerance than c. grandis. using isobaric tags for relative and absolute quantification (itraq), we obtained more al toxicity-respon ...201627455238
enhanced acquisition rates of 'candidatus liberibacter asiaticus' by the asian citrus psyllid (hemiptera: liviidae) in the presence of vegetative flush growth in citrus.the asian citrus psyllid preferentially feeds and exclusively reproduces on young, newly emerged flush shoots of citrus. asian citrus psyllid nymphs feed and complete their life stages on these flush shoots. recent studies conducted under greenhouse conditions have shown that the transmission rates of 'candidatus liberibacter asiaticus' (clas), the putative causal agent of huanglongbing disease of citrus, are enhanced when flush shoots are present. however, it is unclear if clas acquisition by m ...201627451998
repellent activity of botanical oils against asian citrus psyllid, diaphorina citri (hemiptera: liviidae).the asian citrus psyllid, diaphorina citri kuwayama, is the insect vector of the pathogen causing huanglongbing. we selected three botanical oils to evaluate behavioral activity against d. citri. in laboratory olfactometer assays, fir oil was repellent to d. citri females, while litsea and citronella oils elicited no response from d. citri females. in choice settling experiments, d. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day relea ...201627429006
spatio-temporal distribution and natural variation of metabolites in citrus fruits.to study the natural variation and spatio-temporal accumulation of citrus metabolites, liquid chromatography tandem mass spectrometry (lc-ms) based metabolome analysis was performed on four fruit tissues (flavedo, albedo, segment membrane and juice sacs) and different citrus species (lemon, pummelo and grapefruit, sweet orange and mandarin). using a non-targeted metabolomics approach, more than 2000 metabolite signals were detected, from which more than 54 metabolites, including amino acids, fla ...201626775938
thiamethoxam and imidacloprid drench applications on sweet orange nursery trees disrupt the feeding and settling behaviour of diaphorina citri (hemiptera: liviidae).chemical control is the method most used for management of diaphorina citri, the vector of the phloem-limited bacteria associated with citrus huanglongbing (hlb) disease. the objectives of this study were to determine the influence of soil-drench applications of neonicotinoids (thiamethoxam and imidacloprid) on the probing behaviour of d. citri on citrus nursery trees, using the electrical penetration graph (epg) technique, and to measure the d. citri settling behaviour after probing on citrus n ...201626694803
field evaluation of plant defense inducers for the control of citrus huanglongbing.huanglongbing (hlb) is currently the most economically devastating disease of citrus worldwide and no established cure is available. defense inducing compounds are able to induce plant resistance effective against various pathogens. in this study the effects of various chemical inducers on hlb diseased citrus were evaluated in four groves (three with sweet orange and one with mandarin) in florida (united states) for two to four consecutive growing seasons. results have demonstrated that plant de ...201626390185
regulation of cuticle formation during fruit development and ripening in 'newhall' navel orange (citrus sinensis osbeck) revealed by transcriptomic and metabolomic profiling.fruit cuticle, which is composed of cutin and wax and biosynthesized during fruit development, plays important roles in the prevention of water loss and the resistance to pathogen infection during fruit development and postharvest storage. however, the key factors and mechanisms regarding the cuticle biosynthesis in citrus fruits are still unclear. here, fruit cuticle of 'newhall' navel orange (citrus sinensis osbeck) was studied from the stage of fruit expansion to postharvest storage from the ...201626795158
transmission rates of ‘ca. liberibacter asiaticus’ by asian citrus psyllid are enhanced by the presence and developmental stage of citrus flush.asian citrus psyllid (diaphorina citri kuwayama) transmits a bacterium ‘candidatus liberibacter asiaticus’ (clas) putatively responsible for a devastating citrus disease known as asiatic huanglongbing (hlb) (citrus greening disease). the psyllid and disease have invaded many citrus-growing regions including the united states, where the disease is seriously jeopardizing the florida citrus industry. we recently concluded research that showed clas transmission rates are increased when citrus flush ...201626884596
the use of laser light to enhance the uptake of foliar-applied substances into citrus (citrus sinensis) leaves.uptake of foliar-applied substances across the leaf cuticle is central to world food production as well as for physiological investigations into phloem structure and function. yet, despite the presence of stomata, foliar application as a delivery system can be extremely inefficient due to the low permeability of leaf surfaces to polar compounds.201626819863
olfactory response of anastrepha striata (diptera: tephritidae) to guava and sweet orange volatiles.the behavioral responses of virgin and mated female anastrepha striata schiner (diptera: tephritidae) to guava (psidium guajava l.) or sweet orange (citrus sinensis l.) were evaluated separately using multilure traps in two-choice tests in field cages. the results showed that flies were more attracted to guava and sweet orange volatiles than to control (unbaited trap). the physiological state (virgin or mated) of females did not affect their attraction to the fruit volatiles. combined analysis o ...201625800723
the effect of gender and ethnicity on children's attitudes and preferences for essential oils: a pilot study.aromatherapy is frequently recommended for children but children's preferences for specific essential oils are not well documented.201617681258
[effectiveness of antimicrobial formulations for acne based on orange (citrus sinensis) and sweet basil (ocimum basilicum l) essential oils].currently, the antimicrobial resistance has developed in bacterial strains involved in the development of acne. therefore, alternatives to antibiotic treatment have become necessary.201623235794
costs and benefits of insecticide and foliar nutrient applications to huanglongbing-infected citrus trees.the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: psyllidae), vectors candidatus liberibacter asiaticus, which causes huanglongbing (hlb). in florida, hlb incidence is approaching 100% statewide. yields have decreased and production costs have increased since 2005. despite this, some growers are maintaining a level of production and attribute this in part to aggressive psyllid control and foliar nutrition sprays. however, the value of these practices is debated. a replicated ...201727427170
laser-induced fluorescence spectroscopy (lifs) for discrimination of genetically close sweet orange accessions ( citrus sinensis l. osbeck).although there is substantial diversity among cultivated sweet oranges genotypes with respect to morphological, physiological, and agronomic traits, very little variation at dna level has been observed. it is possible that this low dna molecular variability is due to a narrow genetic basis commonly observed in this citrus group. the most different morphological characters observed were originated through mutations, which are maintained by vegetative propagation. despite all molecular tools avail ...201727402688
impact of d-limonene synthase up- or down-regulation on sweet orange fruit and juice odor perception.citrus fruits are characterized by a complex mixture of volatiles making up their characteristic aromas, being the d-limonene the most abundant one. however, its role on citrus fruit and juice odor is controversial. transgenic oranges engineered for alterations in the presence or concentration of few related chemical groups enable asking precise questions about their contribution to overall odor, either positive or negative, as perceived by the human nose. here, either down- or up-regulation of ...201727664619
physiologic, anatomic, and gene expression changes in citrus sunki, poncirus trifoliata, and their hybrids after 'candidatus liberibacter asiaticus' infection.huanglongbing (hlb) is a destructive disease of citrus caused by phloem-limited bacteria, namely 'candidatus liberibacter asiaticus' (las), 'candidatus liberibacter africanus', and 'candidatus liberibacter americanus'. although there are no known hlb-resistant citrus species, studies have reported poncirus trifoliata as being more tolerant. assuming that callose deposition in the phloem of infected plants can inhibit translocation of photosynthetic products and cause starch accumulation, we comp ...201728068188
deficit irrigation and maturation stage influence quality and flavonoid composition of 'valencia' orange fruit.effects of continuous deficit irrigation (di) and partial rootzone drying (prd) treatments (50% etc) in comparison with full irrigation (ci, 100% etc) were investigated during 'valencia' orange fruit maturation. ultra-high-performance liquid chromatography/high-resolution mass spectrometry was used to quantify hesperidin, narirutin, tangeritin, nobiletin, didymin and neoeriocitrin in the fruit juice and peel.201727528197
microrna annotation of plant genomes - do it right or not at all.micrornas are non-coding regulators of gene expression and key factors in development, disease, and targets for bioengineering. consequently, micrornas have become essential elements of already burgeoning draft plant genome descriptions where their annotation is often particularly poor, contributing unduly to the corruption of public databases. using the citrus sinensis as an example, we highlight and review common failings of mirnaome annotations. understanding and exploiting the role of mirnas ...201728054378
assessing a traceability technique in fresh oranges (citrus sinensis l. osbeck) with an hs-spme-gc-ms method. towards a volatile characterisation of organic oranges.a targeted approach using hs-spme-gc-ms was performed to compare flavour compounds of 'navelina' and 'salustiana' orange cultivars from organic and conventional management systems. both varieties of conventional oranges showed higher content of ester compounds. on the other hand, higher content of some compounds related with the geranyl-diphosphate pathway (neryl and geranyl acetates) and some terpenoids were found in the organic samples. furthermore, the partial least square discriminant analys ...201727979182
treatment of textile industry effluents using orange waste: a proposal to reduce color and chemical oxygen demand.various agricultural residues have been tested as biosorbents due to their low cost, high surface area, and favorable surface chemistry. in this work, a sweet orange albedo was tested as a biosorbent for treatment of real textile effluents. the orange albedo powder was prepared by drying the residue at 50 °c and milling to 30 mesh, and then used for dye adsorption from a alkaline (ph = 10.71) effluent. the adsorption process was studied in batch experiments at 30 °c by measuring color removal an ...201727533873
natural compounds from mexican medicinal plants as potential drug leads for anti-tuberculosis drugs.in mexican traditional medicine 187 plant species are used in the treatment of respiratory conditions that may be associated with tuberculosis. in this contribution, we review the ethnobotany, chemistry and pharmacology of 63 species whose extracts have been assayed for antimycobacterial activity in vitro. among these, the most potent is aristolochia brevipes (mic= 12.5 µg/ml), followed by aristolochia taliscana, citrus sinensis, chrysactinia mexicana, persea americana, and olea europaea (mic<64 ...201728198919
citrus leprosis virus n: a new dichorhavirus causing citrus leprosis disease.citrus leprosis (cl) is a viral disease endemic to the western hemisphere that produces local-necrotic and -chlorotic lesions on leaves, branches and fruits and causes serious yield reduction in citrus orchards. samples of sweet orange (citrus × sinensis) trees showing cl symptoms were collected during a survey in non-commercial citrus areas in the southeast region of brazil in 2013-2016. transmission electron microscopy analyses of foliar lesions confirmed the presence of rod-like viral particl ...201728398876
encapsulation of polymethoxyflavones in citrus oil emulsion-based delivery systems.the purpose of this work was to elucidate the effects of citrus oil type on polymethoxyflavone (pmf) solubility and on the physicochemical properties of pmf-loaded emulsion-based delivery systems. citrus oils were extracted from mandarin, orange, sweet orange, and bergamot. the major constituents were determined by gc/ms: sweet orange oil (97.4% d-limonene); mandarin oil (72.4% d-limonene); orange oil (67.2% d-limonene); and bergamot oil (34.6% linalyl acetate and 25.3% d-limonene). pmf-loaded e ...201728182421
cytological and molecular characterization of carotenoid accumulation in normal and high-lycopene mutant oranges.ripe cara cara sweet orange contains 25 times as much carotenoids in flesh as newhall sweet orange, due to high accumulation of carotenes, mainly phytoene, lycopene and phytofluene. only yellow globular chromoplasts were observed in newhall flesh. distinct yellow globular and red elongated crystalline chromoplasts were found in cara cara but only one type of chromoplast was present in each cell. the red crystalline chromoplasts contained lycopene as a dominant carotenoid and were associated with ...201728396598
the effect of inhalation of citrus sinensis flowers and mentha spicata leave essential oils on lung function and exercise performance: a quasi-experimental uncontrolled before-and-after study.recently, there has been an increased interest in the effects of essential oils on athletic performances and other physiological effects. this study aimed to assess the effects of citrus sinensis flower and mentha spicata leaves essential oils inhalation in two different groups of athlete male students on their exercise performance and lung function.201727688737
bioactive compounds in blood oranges (citrus sinensis (l.) osbeck): level and intake.both the composition and the intake of antioxidants (anthocyanins, ascorbic acid and hydroxycinnamic acids) were reported for all blood oranges including the single cultivars (moro, tarocco and sanguinello) and industrially produced juices. the mean values of the studied bioactive compounds in the edible part oranges were: 9.6mg/100g of orange edible part for the anthocyanins; 8.1, 0.7, 1.3, 3.8, 2.5mg/100g for total hydroxycinnamic acids, caffeic, sinapic, ferulic and coumaric acids, respective ...201727542451
depletion of abscisic acid levels in roots of flooded carrizo citrange (poncirus trifoliata l. raf. × citrus sinensis l. osb.) plants is a stress-specific response associated to the differential expression of pyr/pyl/rcar receptors.soil flooding reduces root abscisic acid (aba) levels in citrus, conversely to what happens under drought. despite this reduction, microarray analyses suggested the existence of a residual aba signaling in roots of flooded carrizo citrange seedlings. the comparison of aba metabolism and signaling in roots of flooded and water stressed plants of carrizo citrange revealed that the hormone depletion was linked to the upregulation of csaog, involved in aba glycosyl ester (abage) synthesis, and to a ...201728160166
effect of pasteurization on flavonoids and carotenoids in citrus sinensis (l.) osbeck cv. 'cara cara' and 'bahia' juices.orange juice is considered an excellent dietary source of several bioactive compounds with beneficial properties for human health. citrus sinensis osbeck cv. 'cara cara' is a bud mutation originated from 'washington' navel orange, also known as 'bahia' navel orange. the ascorbic acid, flavonoid, and carotenoid contents in pasteurized and nonpasteurized bahia and cara cara juices using two lc-ms/ms platforms were investigated. higher ascorbic acid content was observed in bahia compared to cara ca ...201728146357
leaf-disc grafting for the transmission of candidatus liberibacter asiaticus in citrus (citrus sinensis; rutaceae) seedlings.the search for resistance/tolerance to the devastating citrus huanglongbing disease (syn. hlb or citrus greening) is generating an increasing number of new plants of diverse genetic makeup. as the increasing number of new plants require more space, resources, and time, the need for faster and more efficient hlb screening tests becomes crucial.201728090406
two previously unknown phytophthora species associated with brown rot of pomelo (citrus grandis) fruits in vietnam.two distinct phytophthora taxa were found to be associated with brown rot of pomelo (citrus grandis), a new disease of this ancestral citrus species, in the vinh long province, mekong river delta area, southern vietnam. on the basis of morphological characters and using the its1-5.8s-its2 region of the rdna and the cytochrome oxidase subunit 1 (coi) as barcode genes, one of the two taxa was provisionally named as phytophthora sp. prodigiosa, being closely related to but distinct from p. insolita ...201728208159
kinetic and isotherm studies of bisphenol a adsorption onto orange albedo(citrus sinensis): sorption mechanisms based on the main albedo components vitamin c, flavones glycosides and carotenoids.orange albedo and its adsorption capacity towards bisphenol a (bpa) were studied. adsorption experiments were conducted in batch mode at 25-55°c. scanning electron microscopy (sem), energy dispersive x-ray spectroscopy (edx), x-ray diffraction (xrd), brunauer-emmett-teller (bet) and fourier transform infrared (ftir) spectroscopy were used to characterise the biosorbent. the effects of various parameters including adsorption time, equilibrium ph, adsorbent dosage and initial adsorbate concentrati ...201728394738
comparison of two dna extraction protocols from leave samples of cotinus coggygria, citrus sinensis and genus juglans.high quality dna is essential for molecular research. secondary metabolites can affect the quantity and quality dna. in current research two dna isolation methods including ctab and delaporta (protocols 1 &amp; 2 respectively) were applied in three leave samples from cotinus coggygria, citrus sinensis and genus juglans that their leaves are rich of secondary metabolites. we successfully isolated dna from c. coggygria, c. sinensis and genus juglans using the two protocols described above. good qu ...201728364796
one target, two mechanisms: the impact of candidatus liberibacter asiaticus and its vector, diaphorina citri on citrus leaf pigments.huanglongbing is currently the largest threat to global citrus production. we examined the effect of hlb pathogen, candidatus liberibacter asiaticus-infection and/or its vector, diaphorina citri-infestation on valencia sweet orange leaf pigments using hplc, followed by gene expression analysis for 46 involved genes in carotenoids- and chlorophylls-biosynthesis pathways. both clas and d. citri alter the total citrus leaf pigments balance with a greater impact by clas. while zeaxanthin was accumul ...201728358623
genome-wide identification and functional analysis of s-rnase involved in the self-incompatibility of citrus.s-rnase-based self-incompatibility is found in solanaceae, rosaceae, and scrophulariaceae, and is the most widespread mechanism that prevents self-fertilization in plants. although 'shatian' pummelo (citrus grandis), a traditional cultivated variety, possesses the self-incompatible trait, the role of s-rnases in the self-incompatibility of 'shatian' pummelo is poorly understood. to identify genes associated with self-incompatibility in citrus, we identified 16 genes encoding homologs of ribonucl ...201727933381
accurate measurement of transgene copy number in crop plants using droplet digital pcr.genetic transformation is a powerful means for the improvement of crop plants, but requires labor- and resource-intensive methods. an efficient method for identifying single-copy transgene insertion events from a population of independent transgenic lines is desirable. currently, transgene copy number is estimated by either southern blot hybridization analyses or quantitative polymerase chain reaction (qpcr) experiments. southern hybridization is a convincing and reliable method, but it also is ...201728231382
functional and structural characterization of a (+)-limonene synthase from citrus sinensis.terpenes make up the largest and most diverse class of natural compounds and have important commercial and medical applications. limonene is a cyclic monoterpene (c10) present in nature as two enantiomers, (+) and (-), which are produced by different enzymes. the mechanism of production of the (-)-enantiomer has been studied in great detail, but to understand how enantiomeric selectivity is achieved in this class of enzymes, it is important to develop a thorough biochemical description of enzyme ...201728272875
antihyperlipidemic effects of citrus sinensis, citrus paradisi, and their combinations.hyperlipidemia, extensively contributes in the progression of coronary heart diseases and atherosclerosis, but may be managed through alterations in the nutritional pattern. several studies show that diet rich in polyphenols and antioxidants have antiatherogenic effects. citrus sinensis and citrus paradisi are widely known for health benefits and have found to produce antioxidant, anti-inflammatory, and hypolipidemic effects, hence current research was planned to determine the hypolipidemic effe ...201727134462
fluorescent carbon nanoparticles from citrus sinensis as efficient sorbents for pollutant dyes.here, we report a simple, green and economic process for the synthesis of highly fluorescent carbon nanoparticles (cps) through low-temperature carbonization of a fruit waste, citrus sinensis peel. this approach allows the large-scale production of aqueous cps dispersions without any additives and post-treatment processes. the as-prepared cps were of small particle size, exhibited bright blue fluorescence under uv irradiation (λmax  = 365 nm) with excellent colloidal stability in water. the chem ...201727321808
identification of potential targets for an anticoagulant pectin.heparin is a sulfated polysaccharide of animal origin showing excellent anticoagulant properties. although it strongly inhibits the coagulation cascade, its interaction with multiple sites results in several side effects. an ideal alternative compound should not only possess anticoagulant and antithrombotic activities, but also provide specific binding to components of the coagulation cascade to decrease side effects and facilitate the control of pharmacologic actions in patient's body. in this ...201727318178
fresh-squeezed orange juice properties before and during in vitro digestion as influenced by orange variety and processing method.this study aims to analyze the influence of processing and orange variety on initial quality, antioxidant activity, total polyphenol content (tpc), and ascorbic acid content of fresh-squeezed orange juice during in vitro digestion. fresh-squeezed orange juice was made from fukumoto navel, lane late navel, olinda nucellar valencia, and campbell valencia oranges, and was processed thermally and nonthermally. antioxidant activity (frap and abts assays), tpc (folin-ciocalteu method), and ascorbic ac ...201728833183
antioxidant and anticancer activities of essential oil from gannan navel orange peel.china is one of the leading producers of citrus in the world. gannan in jiangxi province is the top navel orange producing area in china. in the present study, an essential oil was prepared by cold pressing of gannan navel orange peel followed by molecular distillation. its chemical composition was analyzed by gc-ms. twenty four constituents were identified, representing 97.9% of the total oil. the predominant constituent was limonene (74.6%). the anticancer activities of this orange essential o ...201728829378
anticoagulant and antithrombotic effects of chemically sulfated fucogalactan and citrus pectin.citrus pectin (cp14) from citrus sinensis, and a fucogalactan (e) and a glucan (g16) from agaricus bisporus were isolated and structurally characterized. cp14 was constituted by (1→4)-linked α-d-galpa units, e was composed by a (1→6)-linked α-d-galp main-chain, partially substituted at o-2 by non-reducing end-units of α-l-fucp or α-d-galp, and partially methylated at o-3, whereas g16 was composed of (1→6)-linked β-d-glcp units. the polysaccharides were sulfated giving rise to cp14s, esl and g16s ...201728821126
effects of preheating and storage temperatures on aroma profile and physical properties of citrus-oil emulsions.citrus oils are used as good carrier oil for emulsion fabrication due to their special flavor and various health-promoting functions. in this study, the effects of preheating temperature (30, 40, 50, 60, and 70 °c) and storage temperature (4, 25, and 37 °c) on aroma profiles and physical properties of three citrus-oil (i.e., mandarin, sweet orange, and bergamot oils) emulsions were systematically investigated for the first time. the results demonstrated the significant impact of temperature on a ...201728820942
protective effects of orange (citrus sinensis l.) peel aqueous extract and hesperidin on oxidative stress and peptic ulcer induced by alcohol in rat.massive alcohol drinking can lead to gastric ulcer. in the present study we investigated the gastroprotective effect of citrus sinensis peel aqueous extract (cspe) and hesperidin (h) in ethanol (etoh) induced oxidative stress and peptic ulcer in rats.201728806973
anthocyanin biosynthesis and accumulation in blood oranges during postharvest storage at different low temperatures.blood oranges require low temperature for anthocyanin production. we have investigated the activation of anthocyanin biosynthesis and accumulation in the pulp of moro blood and pera blond oranges (citrus sinensis l. osbeck) stored at either 4 or 9°c after harvesting. both temperatures stimulated anthocyanin accumulation in blood but not in blond oranges. nonetheless, blood orange fruits stored at 9°c reached a darker purple coloration, higher anthocyanin contents and enhanced upregulation of gen ...201728764055
genome-wide identification of sweet orange (citrus sinensis) metal tolerance proteins and analysis of their expression patterns under zinc, manganese, copper, and cadmium toxicity.plant metal tolerance proteins (mtps) play important roles in heavy metal homeostasis; however, related information in citrus plants is limited. citrus genome sequencing and assembly have enabled us to perform a systematic analysis of the mtp gene family. we identified 12 mtp genes in sweet orange, which we have named as citmtp1 and citmtp3 to citmtp12 based on their sequence similarity to arabidopsis thaliana mtps. the citmtps were predicted to encode proteins of 864 to 2556 amino acids in leng ...201728760553
navel orange peel hydroethanolic extract, naringin and naringenin have anti-diabetic potentials in type 2 diabetic rats.the therapy of type 2 diabetes mellitus (t2dm) stays a challenging issue. during the last decade, there has been an interest in the expansion of anti-diabetic drugs especially those of natural sources. thus, the aim of this study was to assess the anti-hyperglycemic and the anti-hyperlipidemic effects as well as the anti-oxidant activities of navel orange hydroethanolic extract and its constituting flavonoids naringin and naringenin on nicotineamide (na)/streptozotocin (stz)-induced type 2 diabe ...201728759757
physicochemical and sensory properties of cold pressed oils from florida hamlin and valencia oranges affected by huanglongbing.cold pressed oils from huanglongbing (hlb) symptomatic (sy) and asymptomatic (as) hamlin and valencia oranges were assessed for 2 y (2014 to 2015 and 2015 to 2016 seasons) with 2 harvest dates for each orange variety per year. physicochemical properties (optical rotation, aldehyde content, ultraviolet [uv] absorbance, refractive index, and specific gravity) mandated by the united states pharmacopeia (usp) for orange oil quality were assessed. hamlin and valencia oils showed minor differences in ...201728759106
mir3954 is a trigger of phasirnas that affects flowering time in citrus.in plant, a few 22-nt mirnas direct cleavages of their targets and trigger the biogenesis of phased small interfering rnas (phasirnas) in plant. in this study, we characterized a mirna triggering phasirnas generation, mir3954, and explored its downstream target genes and potential function. our results demonstrated that mir3954 showed specific expression in the flowers of citrus species, and it targeted a nac transcription factor (cs7 g22460) and two non-coding rna transcripts (lncrnas, cs1 g096 ...201728749585
phylogenetic studies and comparative chloroplast genome analyses elucidate the basal position of halophyte nitraria sibirica (nitrariaceae) in the sapindales.nitraria sibirica is a halophyte and belongs to the family nitrariaceae. the chloroplast genome of nitraria sibirica (159,466 bp) has a quadripartite structure, which consists of a large single-copy (87,914 bp) region, a small single-copy (18,316 bp) region, and a pair of inverted repeats (26,618 bp). sequencing analyses indicate that the chloroplast genome contains 113 distinct genes, including 79 peptide-coding genes, 30 transfer rna genes, and 4 ribosomal rna genes. we also identified 105 per ...201728712318
enzymatic process enhances the flavour profile and increases the proportion of esters in citrus essential oils.citrus essential oils (ceos) are important flavors in the food and confectionary industries. a lipase process was proposed for enhancing the flavor profiles and increasing the proportions of esters in ceos. the effects of the enzymatic process were explored by detecting the constituents of the ceos of american sweet orange oil (aso) and brazil mandarin oil (bmo) through gc/ms and sensory evaluation by a trained panel, and positive effects were confirmed by both methods. a further eleven kinds of ...201728672071
magnesium-deficiency-induced alterations of gas exchange, major metabolites and key enzymes differ among roots, and lower and upper leaves of citrus sinensis seedlings.magnesium (mg)-deficiency is a widespread problem adversely affecting the quality and yield of crops, including citrus. 'xuegan' [citrus sinensis (l.) osbeck] seedlings were irrigated every other day with nutrient solution at an mg concentration of 0 mm (mg-deficiency) or 1 mm (mg-sufficiency) for 16 weeks. thereafter, biomass, leaf mass per area, ribulose-1,5-bisphosphate carboxylase/oxygenase (rubisco), pigments in the upper and lower leaves, mg, gas exchange, organic acids, nonstructural carb ...201728633366
spray pesticide applications in mediterranean citrus orchards: canopy deposition and off-target losses.only a portion of the water volume sprayed is deposited on the target when applying plant protection products with air-assisted axial-fan airblast sprayers in high growing crops. a fraction of the off-target losses deposits on the ground, but droplets also drift away from the site. this work aimed at assessing the spray distribution to different compartments (tree canopy, ground and air) during pesticide applications in a mediterranean citrus orchard. standard cone nozzles (teejet d3 dc35) and v ...201728525940
metabolomic response to huanglongbing: role of carboxylic compounds in citrus sinensis response to 'candidatus liberibacter asiaticus' and its vector, diaphorina citri.huanglongbing, a destructive disease of citrus, is caused by the fastidious bacterium 'candidatus liberibacter asiaticus' and transmitted by asian citrus psyllid, diaphorina citri. the impact of 'ca. l. asiaticus' infection or d. citri infestation on valencia sweet orange (citrus sinensis) leaf metabolites was investigated using gas chromatography mass spectrometry, followed by gene expression analysis for 37 genes involved in jasmonic acid (ja), salicylic acid (sa), and proline-glutamine pathwa ...201728510485
development of monoclonal antibody-based elisa for the quantification of orange allergen cit s 2 in fresh and processed oranges.the aim of this study was to develop a monoclonal antibody (mab)-based enzyme-linked immunosorbent assay (elisa) for the quantification of a major allergen (cit s 2) in fresh and processed oranges. purified recombinant cit s 2 (rcit s 2)-small ubiquitin-like modifier (sumo) was used for the production of mabs. in the optimized elisa, the recovery of rcit s 2 from navel oranges or orange juice was 107-132%, and the intra- and inter-assay coefficients of variation were 3.1-8.8% and 4.4-11%, respec ...201728490094
systems biology study of transcriptional and post-transcriptional co-regulatory network sheds light on key regulators involved in important biological processes in citrus sinensis.transcriptional and post-transcriptional regulators including transcription regulator, transcription factor and mirna are the main endogenous molecular elements which control complex cellular mechanisms such as development, growth and response to biotic and abiotic stresses in a coordinated manner in plants. utilizing the most recent information on such relationships in a plant species, obtained from high-throughput experimental technologies and advanced computational tools, we can reconstruct i ...201728461722
fumigant activity of sweet orange essential oil fractions against red imported fire ants (hymenoptera: formicidae).sweet orange oil fractions were prepared by molecular distillation of cold-pressed orange oil from sample a (citrus sinensis (l.) 'hamlin' from america) and sample b (citrus sinensis osbeck 'newhall' from china) respectively, and their fumigant activities against medium workers of red imported fire ants (solenopsis invicta buren) were investigated. the volatile composition of the orange oil fractions was identified and quantified using gc-ms. fractions from sample a (a1, a2, and a3) contained 23 ...201728444322
bioavailable citrus sinensis extract: polyphenolic composition and biological activity.citrus plants contain large amounts of flavonoids with beneficial effects on human health. in the present study, the antioxidant and anti-inflammatory potential of bioavailable polyphenols from citrus sinensis was evaluated in vitro and ex vivo, using the murine macrophages cell line j774a.1 and primary peritoneal macrophages. following simulated gastro-intestinal digestion, the in vitro bioavailability of citrus sinensis polyphenolic extract was assessed using the human cell line caco-2 grown a ...201728420125
transcriptome changes associated with boron deficiency in leaves of two citrus scion-rootstock combinations.boron (b) deficiency stress is frequently observed in citrus orchards and causes considerable loss of productivity and fruit quality. carrizo citrange (cc) has been reported as a rootstock more tolerant to b deficiency than trifoliate orange (to). the 'newhall' navel orange (ns) performed better when grafted onto cc (ns/cc) than when grafted onto to (ns/to) under long-term b deficiency. the present study confirmed that ns/cc had higher boron content, leaf fresh weight, lower leaf chlorosis and s ...201728352276
photodynamic inactivation of conidia of the fungus colletotrichum abscissum on citrus sinensis plants with methylene blue under solar radiation.antimicrobial photodynamic treatment (apdt) is a promising light based approach to control diseases caused by plant-pathogenic fungi. in the present study, we evaluated the effects of apdt with the phenothiazinium photosensitizer methylene blue (mb) under solar radiation on the germination and viability of conidia of the pathogenic fungus colletotricum abscissum (former colletotrichum acutatum sensu lato). experiments were performed both on petals and leaves of sweet orange (citrus sinensis) in ...201728941778
microrna-mediated responses to long-term magnesium-deficiency in citrus sinensis roots revealed by illumina sequencing.magnesium (mg)-deficiency occurs most frequently in strongly acidic, sandy soils. citrus are grown mainly on acidic and strong acidic soils. mg-deficiency causes poor fruit quality and low fruit yield in some citrus orchards. for the first time, we investigated mg-deficiency-responsive mirnas in 'xuegan' (citrus sinensis) roots using illumina sequencing in order to obtain some mirnas presumably responsible for citrus mg-deficiency tolerance.201728836935
bio-pesticides: new tool for the control of aedes (stegomyia) albopictus (culicidae: diptera) in pakistan.application of plant extracts as mosquito control strategy was practiced from centuries. these are easily available, non-toxic, biodegradable and exhibit broad-spectrum target specific activities against larval stages of mosquitoes.201729062852
effects of transgenic expression of brevibacterium linens methionine gamma lyase (mgl) on accumulation of tylenchulus semipenetrans and key aminoacid contents in carrizo citrange.carrizo transgenic plants overexpressing methionine-gamma-lyase produced dimethyl sulfide. the transgenic plants displayed more resistance to nematode attacks (tylenculus semipenetrans) and may represent an innovative strategy for nematode control. tylenchulus semipenetrans is a nematode pest of many citrus varieties that causes extensive damage to commercial crops worldwide. carrizo citrange vr. (citrus sinensis l. usb × poncirus trifoliate l. raf) plants overexpressing brevibacterium linens me ...201729058103
selection of reference genes for expression studies in diaphorina citri (hemiptera: liviidae).the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is considered the main vector of the bacteria associated with huanglongbing, a very serious disease that has threatened the world citrus industry. the absence of efficient control management protocols, including a lack of resistant cultivars, has led to the development of different approaches to study this pathosystem. the production of resistant genotypes relies on d. citri gene expression analyses by rt-qpcr to assess c ...201729029285
transcription factor citerf71 activates the terpene synthase gene cittps16 involved in the synthesis of e-geraniol in sweet orange fruit.the unique flavor of citrus fruit depends on complex combinations of soluble sugars, organic acids, and volatile compounds. the monoterpene e-geraniol is an important volatile, contributing to flavor in sweet orange (citrus sinensis osbeck). moreover, antifungal activity of e-geraniol has also been observed. however, the terpene synthase (tps) responsible for its synthesis has not been identified in sweet orange. terpene synthase 16 (cittps16) was shown to catalyze synthesis of e-geraniol in vit ...201728992329
effect of aromatherapy via inhalation on the sleep quality and fatigue level in people undergoing hemodialysis.the most common problems in hemodialysis patients are sleep disorders and fatigue. this randomized-controlled experimental study was conducted to determine the effect of aromatherapy applied by inhalation on sleep quality and fatigue level in hemodialysis patients. the study was completed in five hemodialysis centers settled in two provinces with 27 intervention group patients and 35 controls, being totally 62 patients, recruited with simple randomization. ethical approval, informed consent from ...201728985917
different adaptation strategies of two citrus scion/rootstock combinations in response to drought stress.scion/rootstock interaction is important for plant development and for breeding programs. in this context, polyploid rootstocks presented several advantages, mainly in relation to biotic and abiotic stresses. here we analyzed the response to drought of two different scion/rootstock combinations presenting different polyploidy: the diploid (2x) and autotetraploid (4x) rangpur lime (citrus limonia, osbeck) rootstocks grafted with 2x valencia delta sweet orange (citrus sinensis) scions, named v/2xr ...201728545114
comparative transcriptome and proteome profiling of two citrus sinensis cultivars during fruit development and ripening.transcriptome and proteome analyses on fruit pulp from the blood orange 'zaohong' and the navel orange 'twenty-first century' were performed to study citrus sinensis quality-related molecular changes during consecutive developmental periods, including young fruit, fruit-coloring onset and fruit delayed-harvest for two months, during which fruit remained on the trees.201729268697
chemical composition of the essential oils isolated from peel of three citrus species and their mosquitocidal activity against culex pipiens.three essential oils (eos) were isolated from the peel of citrus fruits citrus reticulata l., citrus reticulata chinase blanco and citrus sinensis (l.) osbeck (family: rutaceae) and evaluated against culex pipiens l.1758 (family: culicidae). chemical composition indicated that the eos were rich in essential phytochemicals including hydrocarbons, monoterpenes and sesquiterpenes. these constituents revealed some variability among the oils displaying interesting chemotypes limonene (35-51%), 1r-α-p ...201729017021
flavonoid composition of tarocco (citrus sinensis l. osbeck) clone "lempso" and fast antioxidant activity screening by dpph-uhplc-pda-it-tof.clonal selection and hybridisation are valid strategies to obtain fruits with enhanced sensorial and nutraceutical properties. within citrus sinensis varieties, tarocco clone "lempso" is a typical product of the calabria region (italy) characterised by its red pulp. this is the first report concerning its accurate profiling.201728686312
natural occurrence of aldol condensation products in valencia orange oil.cold pressed orange oils contain predominantly d-limonene (approximately 95%) and various other lower concentration monoterpenes, sesquiterpenes, sinensals plus 3 key aliphatic aldehydes: hexanal, octanal. and decanal. the aldol self-condensation products or "dimers" for each aldehyde have been postulated as being present at low concentrations in the oil. however, to date only the hexanal dimer has been previously reported. in this paper, cold pressed valencia orange oil was fractionally distill ...201729095499
oviposition of fruit flies (diptera: tephritidae) and its relation with the pericarp of citrus fruits.fruit flies (diptera: tephritidae) represent a threat to fruit growing worldwide, mainly the citrus culture, however, biological studies show that fruit flies are not perfectly adapted to this host. this study investigated oviposition of anastrepha fraterculus (wiedemann, 1830) and ceratitis capitata (wiedemann, 1824) and its relation with the pericarp of citrus fruits. we evaluated the relationship between depth of oviposition of a. fraterculus and c. capitata and epicarp thickness of orange [c ...201729091115
transcriptional analysis of sweet orange trees co-infected with 'candidatus liberibacter asiaticus' and mild or severe strains of citrus tristeza virus.citrus worldwide is threatened by huanglongbing (hlb) and tristeza diseases caused by 'candidatus liberibacter asiaticus' (calas) and citrus tristeza virus (ctv). although the pathogens are members of the α-proteobacteria and closteroviridae, respectively, both are restricted to phloem cells in infected citrus and are transmitted by insect vectors. the response of sweet orange to single infection by either of these two pathogens has been characterized previously by global gene expression analysi ...201729089035
co-infection of sweet orange with severe and mild strains of citrus tristeza virus is overwhelmingly dominated by the severe strain on both the transcriptional and biological levels.citrus tristeza is one of the most destructive citrus diseases and is caused by the phloem-restricted closterovirus, citrus tristeza virus. mild strain ctv-b2 does not cause obvious symptoms on indicators whereas severe strain ctv-b6 causes symptoms, including stem pitting, cupping, yellowing, and stiffening of leaves, and vein corking. our laboratory has previously characterized changes in transcription in sweet orange separately infected with ctv-b2 and ctv-b6. in the present study, transcript ...201728912786
long-term manganese-toxicity-induced alterations of physiology and leaf protein profiles in two citrus species differing in manganese-tolerance.manganese (mn)-intolerant 'sour pummelo' (citrus grandis) and mn-tolerant 'xuegan' (citrus sinensis) seedlings were irrigated for 17 weeks with 2 (control) or 600μm (mn-toxicity or -excess) mnso4. c. sinensis had higher mn-tolerance than c. grandis, as indicated by the higher photosynthesis rates in mn-excess c. sinensis leaves. under mn-toxicity, mn levels were similar between c. sinensis and c. grandis roots, but lower in c. sinensis leaves than in c. grandis leaves. this might be responsible ...201728910703
antibacterial and antibiotic-modifying activity of methanol extracts from six cameroonian food plants against multidrug-resistant enteric bacteria.the present work was designed to investigate the antibacterial activities of methanol extracts from six cameroonian edible plants and their synergistic effects with some commonly used antibiotics against multidrug-resistant (mdr) gram-negative bacteria expressing active efflux pumps. the extracts were subjected to qualitative phytochemical screening and the microdilution broth method was used for antibacterial assays. the results of phytochemical tests indicate that all tested crude extracts con ...201728904944
comparative transcriptome analysis during early fruit development between three seedy citrus genotypes and their seedless mutants.identification of genes with differential transcript abundance (gdta) in seedless mutants may enhance understanding of seedless citrus development. transcriptome analysis was conducted at three time points during early fruit development (phase 1) of three seedy citrus genotypes: fallglo (bower citrus hybrid (citrus reticulata×c. reticulata×c. paradisi)×temple (c. reticulata×c. sinensis)), grapefruit (c. paradisi), pineapple sweet orange (c. sinensis), and their seedless mutants. seed abortion in ...201728904803
influence of natural and synthetic vitamin c (ascorbic acid) on primary and secondary metabolites and associated metabolism in quinoa (chenopodium quinoa willd.) plants under water deficit regimes.phytoextracts are being widely used these days as a source of bioactive compounds for mitigating the harmful effects of abiotic stresses including drought stress. in this study, it was assessed how far foliar applied pure synthetic ascorbic acid (asa) or natural sweet orange juice (oj) enriched with asa could mitigate the drought stress induced adverse effects on growth and some key metabolic processes in quinoa (chenopodium quinoa willd.; cultivar v9) plants. two weeks old quinoa seedlings were ...201729248677
characterization of mycosphaerellaceae species associated with citrus greasy spot in panama and spain.greasy spot of citrus, caused by zasmidium citri-griseum (= mycosphaerella citri), is widely distributed in the caribbean basin, inducing leaf spots, premature defoliation, and yield loss. greasy spot-like symptoms were frequently observed in humid citrus-growing regions in panama as well as in semi-arid areas in spain, but disease aetiology was unknown. citrus-growing areas in panama and spain were surveyed and isolates of mycosphaerellaceae were obtained from citrus greasy spot lesions. a sele ...201729236789
does the fragrance of essential oils alleviate the fatigue induced by exercise? a biochemical indicator test in rats.to study the effect of the essential oils of citrus sinensis l., mentha piperita l., syzygium aromaticum l., and rosmarinus officinalis l. on physical exhaustion in rats.201729234408
phylogenetic study of the colletotrichum species on imported citrus fruits uncovers a low diversity and a new species in the colletotrichum gigasporum complex.colletotrichum species associated with citrus fruits are fragmentarily known and it lacks accordingly accurate information on the diversity carried alongside the trade of these commodities from producer countries to europe. in this study, we investigated the molecular phylogenetic diversity, colonisation, and prevalence of colletotrichum isolated from asymptomatic and diseased tissues of nine citrus fruit species from 17 geographically diverse countries. totally 454 isolates were morphoculturall ...201728889910
reproducible genomic dna preparation from diverse crop species for molecular genetic applications.several high-throughput molecular genetic analyses rely on high-quality genomic dna. copurification of other molecules can negatively impact the functionality of plant dna preparations employed in these procedures. isolating dna from agronomically important crops, such as sugarcane, rice, citrus, potato and tomato is a challenge due to the presence of high fiber, polysaccharides, or secondary metabolites. we present a simplified, rapid and reproducible sds-based method that provides high-quality ...201729213298
repellency potential of essential oils against housefly, musca domestica l.in present study, the essential oils such as mentha piperita (mentha oil, m.o), cymbopogan citratus (lemongrass oil, lg.o), citrus sinensis (orange oil, o.o), and eucalyptus globulus (eucalyptus oil, e.o) were evaluated for repellency against housefly (musca domestica) in a specially designed chamber. further, to study any synergistic effect, essential oil combinations, i.e., m.o + lg.o, m.o + o.o, and m.o + e.o, were screened at 50:50 and 70:30 ratios. the results showed superior repellency of ...201729197060
[insecticidal action of synthetic girgensohnine analogues and essential oils on rhodnius prolixus (hemiptera: reduviidae)].the alkaloid girgensohnine has been used as a natural model in the synthesis of new alkaloid-like alpha-aminonitriles with insecticidal effect against disease vectors.201729161477
green synthesis of capped gold nanoparticles and their effect on gram-positive and gram-negative bacteria.we report synthesis of capped gold nanoparticles (c-aunps) of ≈20-30 nm by reducing haucl4 with flower and leaf extracts of ocimum tenuiflorum, leaves of azadirachta indica and mentha spicata and peel of citrus sinensis plants.201729134123
recent advances in understanding xanthomonas citri subsp. citri pathogenesis and citrus canker disease management.taxonomic status: bacteria; phylum proteobacteria; class gammaproteobacteria; order xanthomonadles; family xanthomonadaceae; genus xanthomonas; species xanthomonas citri subsp. citri (xcc). host range: compatible hosts vary in their susceptibility to cc, with grapefruit, lime, and lemon being most susceptible, sweet orange being moderately susceptible, and kumquat and calamondin being among the least susceptible (gottwald et al., 1993). microbiological properties: xcc is a rod-shaped (1.5 - 2.0 ...201729105297
genomic analysis of terpene synthase family and functional characterization of seven sesquiterpene synthases from citrus sinensis.citrus aroma and flavor, chief traits of fruit quality, are derived from their high content in essential oils of most plant tissues, including leaves, stems, flowers, and fruits. accumulated in secretory cavities, most components of these oils are volatile terpenes. they contribute to defense against herbivores and pathogens, and perhaps also protect tissues against abiotic stress. in spite of their importance, our understanding of the physiological, biochemical, and genetic regulation of citrus ...201728883829
characterization and metabolic diversity of flavonoids in citrus species.flavonoids are widely distributed in plants and play important roles in many biological processes. citrus fruits are rich dietary sources of flavonoids. however, there have been very few reports about the comprehensive metabolic profile and natural diversity of flavonoids in different tissues of various citrus cultivars. in this study, based on the 7416 metabolic signals detected with non-targeted metabolomics approach, principal component analysis revealed the flavedo has the largest difference ...201728874745
expression patterns of flowering genes in leaves of 'pineapple' sweet orange [citrus sinensis (l.) osbeck] and pummelo (citrus grandis osbeck).in citrus the transition from juvenility to mature phase is marked by the capability of a tree to flower and fruit consistently. the long period of juvenility in citrus severely impedes the use of genetic based strategies to improve fruit quality, disease resistance, and responses to abiotic environmental factors. one of the genes whose expression signals flower development in many plant species is flowering locus t (ft).201728854897
gene coexpression network analysis of fruit transcriptomes uncovers a possible mechanistically distinct class of sugar/acid ratio-associated genes in sweet orange.the ratio of sugars to organic acids, two of the major metabolites in fleshy fruits, has been considered the most important contributor to fruit sweetness. although accumulation of sugars and acids have been extensively studied, whether plants evolve a mechanism to maintain, sense or respond to the fruit sugar/acid ratio remains a mystery. in a prior study, we used an integrated systems biology tool to identify a group of 39 acid-associated genes from the fruit transcriptomes in four sweet orang ...201729084509
compositional shift in fatty acid profiles of lipids obtained from oleaginous yeasts upon the addition of essential oil from citrus sinensis l.tailoring lipids from oleaginous yeasts to contain specific types of fatty acid is of considerable interest to food, fuel, and pharmaceutical industries. in this study, the essential oil obtained from citrus sinesus l. has been used to alter the fatty acid composition of two common oleaginous yeasts, rhodosporidium toruloides and cryptococcus curvatus. with increasing levels of essential oil in the medium, the metabolic flux of the fatty acid biosynthesis pathway shifted towards saturated fatty ...201728474217
first report of phyllosticta citricarpa and description of two new species, p. paracapitalensis and p. paracitricarpa, from citrus in europe.the genus phyllosticta occurs worldwide, and contains numerous plant pathogenic, endophytic and saprobic species. phyllosticta citricarpa is the causal agent of citrus black spot disease (cbs), affecting fruits and leaves of several citrus hosts (rutaceae), and can also be isolated from asymptomatic citrus tissues. citrus black spot occurs in citrus-growing regions with warm summer rainfall climates, but is absent in countries of the european union (eu). phyllosticta capitalensis is morphologica ...201728720979
quantification of transfer of salmonella from citrus fruits to peel, edible portion, and gloved hands during hand peeling.although studies have quantified bacterial transfer between hands and various materials, cross-contamination between the surface of fresh citrus fruit and the edible portions during hand peeling has not been reported. this study quantifies transfer of salmonella to the edible portion of citrus fruit from a contaminated peel during hand peeling. citrus fruits used for this study were citrus sinensis (sweet orange) cultivars 'valencia' and 'navel', citrus unshiu (satsuma mandarins), citrus reticul ...201728463082
ectopic expression of xylella fastidiosa rpff conferring production of diffusible signal factor in transgenic tobacco and citrus alters pathogen behavior and reduces disease severity.the pathogenicity of xylella fastidiosa is associated with its ability to colonize the xylem of host plants. expression of genes contributing to xylem colonization are suppressed, while those necessary for insect vector acquisition are increased, with increasing concentrations of diffusible signal factor (dsf) whose production is dependent on rpff. we previously demonstrated that transgenic citrus plants ectopically expressing rpff from a citrus strain of x. fastidiosa subsp. pauca exhibited les ...201728777044
proteomics-based identification of differentially abundant proteins reveals adaptation mechanisms of xanthomonas citri subsp. citri during citrus sinensis infection.xanthomonas citri subsp. citri (xac) is the causal agent of citrus canker. a proteomic analysis under in planta infectious and non-infectious conditions was conducted in order to increase our knowledge about the adaptive process of xac during infection.201728693412
isolation and identification of fungi associated with spoilt fruits vended in gwagwalada market, abuja, nigeria.annual reports have shown that 20% of fruits and vegetables produced are lost to spoilage. this study was undertaken to isolate and identify fungi that are associated with spoilt fruits commonly sold in gwagwalada market, abuja, and recommend appropriate control measure.201728507410
identification and characterization of citrus tristeza virus isolates breaking resistance in trifoliate orange in california.most citrus tristeza virus (ctv) isolates in california are biologically mild and symptomless in commercial cultivars on ctv tolerant rootstocks. however, to better define california ctv isolates showing divergent serological and genetic profiles, selected isolates were subjected to deep sequencing of small rnas. full-length sequences were assembled, annotated and trifoliate orange resistance-breaking (rb) isolates of ctv were identified. phylogenetic relationships based on their full genomes pl ...201728453412
reprogramming of a defense signaling pathway in rough lemon and sweet orange is a critical element of the early response to 'candidatus liberibacter asiaticus'.huanglongbing (hlb) in citrus infected by candidatus liberibacter asiaticus (clas) has caused tremendous losses to the citrus industry. no resistant genotypes have been identified in citrus species or close relatives. among citrus varieties, rough lemon (citrus jambhiri) has been considered tolerant due to its ability to produce a healthy flush of new growth after infection. the difference between tolerance and susceptibility is often defined by the speed and intensity of a plant's response to a ...201729214028
the effect of viroid infection of citrus trees on the amoebicidal activity of 'maltese half-blood' (citrus sinensis) against trophozoite stage of acanthamoeba castellanii neff.in order to promote a local tunisian product, this study was designed to examine, for the first time, the anti-acanthamoeba activity (acanthamoeba castellanii neff) of the essential oils of tunisian citrus sinensis peels (maltese half-blood) and the effect of viroid plant infection on this activity. to do so, three samples of peels' essential oils were studied: from a healthy plant (control), a plant inoculated with citrus exocortis viroid (cevd) and one inoculated with hot stunt cachexia viroid ...201728916460
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