Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| multiple abscesses caused by gardnerella vaginalis in an immunocompetent man. | gardnerella vaginalis causing significant infection in men is rare. we report a case of sepsis in a previously well man, who developed a perinephric abscess and empyema. g. vaginalis was isolated after prolonged culture of samples from both sites. the microbiological features of the case are discussed. | 2005 | 16038745 |
| complexity of vaginal microflora as analyzed by pcr denaturing gradient gel electrophoresis in a patient with recurrent bacterial vaginosis. | gardnerella vaginalis has long been the most common pathogen associated with bacterial vaginosis (bv). we aimed to test our hypothesis that symptoms and signs of bv do not necessarily indicate colonization by this organism, and often will not respond to standard metronidazole or clindamycin treatment. | 2005 | 16040324 |
| a cluster analysis of bacterial vaginosis-associated microflora and pelvic inflammatory disease. | controversy surrounds the association between bacterial vaginosis (bv) and pelvic inflammatory disease (pid). women (n = 1,140) were ascertained at five us centers, enrolled (1999-2001), and followed up for a median of 3 years. serial vaginal swabs were obtained for gram's stain and cultures. pid was defined as 1) histologic endometritis or 2) pelvic pain and tenderness plus oral temperature >38.8 degrees c, leukorrhea or mucopus, erythrocyte sedimentation rate >15 mm/hour, white blood cell coun ... | 2005 | 16093289 |
| [significance of clue cells in the diagnosis of male urogenital infection]. | to explore the significance of clue cells in the diagnosis of male urogenital infection. | 2005 | 16138580 |
| microbiologic response to treatment of bacterial vaginosis with topical clindamycin or metronidazole. | to compare the frequencies, concentrations, and antimicrobial susceptibilities of vaginal microbes isolated from women with bacterial vaginosis (bv) before and after therapy, 119 nonpregnant women aged 18 to 45 with clinical and gram stain evidence of bv were randomized to receive intravaginal clindamycin or metronidazole. vaginal swabs were collected at baseline and 7 to 12 days, 35 to 45 days, and 70 to 90 days following therapy for quantitative vaginal culture. for the 99 women completing all ... | 2005 | 16145097 |
| utility of amsel criteria, nugent score, and quantitative pcr for gardnerella vaginalis, mycoplasma hominis, and lactobacillus spp. for diagnosis of bacterial vaginosis in human immunodeficiency virus-infected women. | bacterial vaginosis (bv) is a clinical syndrome presenting with a malodorous vaginal discharge and increased vaginal ph. diagnosis has been based on clinical amsel criteria and direct gram stain of vaginal secretions. human immunodeficiency virus (hiv)-infected participants in the women's interagency hiv study contributed cervicovaginal lavage (cvl) samples. lactobacilli, gardnerella vaginalis, and mycoplasma hominis in cervicovaginal lavage samples were quantified by pcr. gynecologic evaluation ... | 2005 | 16145114 |
| characterization of vaginal microflora of healthy, nonpregnant women by chaperonin-60 sequence-based methods. | the purpose of this study was to use a novel method that was based on the application of chaperonin-60 sequencing to describe the vaginal microflora of 16 healthy women. | 2005 | 16150261 |
| variation in microbiologic profiles among pregnant women with bacterial vaginosis. | the purpose of this study was to determine if clinical findings and sociodemographic variables among bacterial vaginosis (bv)-positive pregnant women are associated with different microbiologic profiles. | 2005 | 16150269 |
| use of dna hybridization to detect vaginal pathogens associated with bacterial vaginosis among asymptomatic pregnant women. | the purpose of this study was to determine whether microbial dna hybridization is a useful method to study bacterial vaginosis in asymptomatic pregnant women. | 2005 | 16150270 |
| a preliminary survey of atopobium vaginae in women attending the dunedin gynaecology out-patients clinic: is the contribution of the hard-to-culture microbiota overlooked in gynaecological disorders? | preliminary studies have indicated that the recently described bacterium atopobium vaginae may have an association with bacterial vaginosis (bv). fifty-five women attending the gynaecology out-patient's clinic were tested for the presence of this micro-organism, gardnerella vaginalis, mobiluncus and bacteroides species by polymerase chain reaction (pcr)-based assays. the frequency of detection was 40%. pcr detection of gardnerella vaginalis with a. vaginae, occurred in 50% of a. vaginae-positive ... | 2005 | 16171487 |
| [sexually transmitted diseases]. | 2005 | 16194497 | |
| comparison between gram stain and culture for the characterization of vaginal microflora: definition of a distinct grade that resembles grade i microflora and revised categorization of grade i microflora. | the microbiological diagnosis of bacterial vaginosis is usually made using nugent's criteria, a useful but rather laborious scoring system based on counting bacterial cell types on gram stained slides of vaginal smears. ison and hay have simplified the score system to three categories and added a fourth category for microflora with a predominance of the streptococcus cell type. because in the nugent system several cell types are not taken into account for a final score, we carried out a detailed ... | 2005 | 16225680 |
| adherent biofilms in bacterial vaginosis. | bacterial vaginosis is a common infectious disorder. although known since ancient times, little progress has occurred in identifying causal factors. our aims were to study the bacterial community structure and the spatial organization of microbiota on the epithelial surfaces of vaginal biopsy specimens. | 2005 | 16260520 |
| bacterial flora of the low male genital tract in patients consulting for infertility. | the physiological aerobic bacterial flora of the low male genital tract was determined. this prospective study was performed on 600 semen specimens collected from 543 asymptomatic males consulting for infertility. semen cultures were sterile in 28.8%, with a polymicrobial flora and/or absence or low titres of ureaplasma urealyticum in 49.3%, and with one or two aerobic and facultative bacteria > or =1 x 10(3) cfu ml(-1) and/or u. urealyticum with titres > or =10(4) ccu ml(-1) (colour changing un ... | 2005 | 16266393 |
| molecular identification of bacteria associated with bacterial vaginosis. | bacterial vaginosis affects millions of women and is associated with several serious health conditions. the cause of bacterial vaginosis remains poorly understood despite numerous studies based on cultures. bacteria in microbial communities can be identified without cultivation by characterizing their ribosomal dna (rdna) sequences. | 2005 | 16267321 |
| [the correlation of inflammation and epithelial changes in the pap smears of cervix uteri]. | a modification of the bethesda system classification of cytologic findings of uterine cervix named zagreb 1990 has been accepted in croatia as a unique classification, the use of which has begun after the publication and printing of the new, uniform method of cytologic examination. aim: the aim of this study was to assess the number and type of cytologic abnormalities of uterine cervix classified according to zagreb 1990, on routinely examined pap smears during the 6-year period, and to investig ... | 2005 | 16334735 |
| prebiotic effects of oligosaccharides on selected vaginal lactobacilli and pathogenic microorganisms. | the purpose of this study was to select endogenous human vaginal lactobacilli strains on the basis of the main probiotic properties observed in the vaginal environment in order to use them for the evaluation of the potential prebiotic properties of oligosaccharides. from vaginal samples of 50 women with a normal flora, 17 lactobacilli strains were first isolated because of their high level of hydrogen peroxide production. then six strains were selected mainly for their ability (i) to adhere to v ... | 2005 | 16701545 |
| [vulvovaginitis: vaginal ph changes and associated microflora]. | to establish a correlation between ph vaginal and the microflora associated in carriers of vulvovaginites. | 2005 | 17768818 |
| [sialidase activity in women with bacterial vaginosis]. | bacterial vaginosis (vb) is a syndrome characterized by overgrowth of endogenous gram negative bacterial flora and the lack of the normal flora. within bacterial enzymes, sialidases have been considered a virulence factor of many pathogenic microorganisms colonizing the different mucous membranes. their presence in vaginal discharges can be correlated with vb. the aim of this study was to detect the activity of this enzyme in women with this syndrome and without clinical evidence of genital infe ... | 2006 | 16715761 |
| clinical characteristics of vaginal discharge in bacterial vaginosis diagnosed by nugent's criteria. | to determine which clinical signs have an important contribution in bacterial vaginosis (bv) diagnosed by nugent's criteria. | 2006 | 16761528 |
| in vitro antibacterial activity of lactobacillus helveticus strain ks300 against diarrhoeagenic, uropathogenic and vaginosis-associated bacteria. | the purpose of this study was to investigate in vitro the antibacterial activity of the lactobacillus helveticus strain ks300 against vaginosis-associated bacteria including gardnerella vaginalis and prevotella bivia, uropathogenic escherichia coli, and diarrhoeagenic salmonella enterica serovar typhimurium. | 2006 | 16907815 |
| the association of atopobium vaginae and gardnerella vaginalis with bacterial vaginosis and recurrence after oral metronidazole therapy. | we investigated associations between atopobium vaginae and bacterial vaginosis (bv) and the role that a. vaginae plays in recurrent bv after oral metronidazole therapy. | 2006 | 16941351 |
| preterm birth due to maternal infection: causative pathogens and modes of prevention. | preterm birth represents a major problem for modern obstetrics due to its increasing frequency and the accompanying socioeconomic impact. although several maternal characteristics related to preterm birth have been identified, the etiology in most cases remains inadequately understood. various microorganisms have been linked to the pathogenesis of preterm birth. microbes may reach the amniotic cavity and fetus by ascending from the vagina and cervix, by hematogenous distribution through the plac ... | 2006 | 16953371 |
| [atopobium vaginae: characterization and association with pathogenesis of bacterial vaginosis]. | atopobium vaginae was described in 1999 by rodriguez et al. it is a gram-positive bacterium producing organic acids (lactic acid, acetic acid, formic acid) as a results of glucose fermentation. it was first found in vaginal swab taken from a healthy woman using 16s rrna analysis. a. vaginae is associated with bacterial vaginosis and its consequences in upper part of women reproductive organs. | 2006 | 16958231 |
| selection and identification of anaerobic lactobacilli producing inhibitory compounds against vaginal pathogens. | two strains of lactobacillus crispatus (15l08 and 21l07) and one strain of lactobacillus jensenii (5l08) were selected from amongst 100 isolates from the vaginas of healthy premenopausal women for properties relevant to mucosal colonization and the production of h2o2 and/or bacteriocin-like compound. all three strains self-aggregated and adhered to vaginal epithelial cells, displacing well-known vaginal pathogens, such as gardnerella vaginalis and candida albicans. lactobacillus crispatus 15l08 ... | 2006 | 16965354 |
| reversible deficiency of antimicrobial polypeptides in bacterial vaginosis. | bacterial vaginosis is a common condition associated with increased risk of sexually transmitted diseases, including human immunodeficiency virus infections. in contrast, vulvovaginal candidiasis has a much weaker association with sexually transmitted diseases. we found that vaginal lavage fluid from women with bacterial vaginosis is deficient in antimicrobial polypeptides and antimicrobial activity compared to fluid from healthy women or women with vulvovaginal candidiasis. effective treatment ... | 2006 | 16988245 |
| etiologic agents of cervicovaginitis in turkish women. | to investigate the distribution of microbiologic agents causing cervicovaginitis. | 2006 | 17013472 |
| clue cell. | 2006 | 17050945 | |
| [vaginosis in patients with antenatal foetus death]. | polymerase chain reaction (pcr) analysis of bacterial vaginosis as well as of vaginal anaerobe flora (mycoplasma hominis, ureaplasma urealyticum, gardnerella vaginalis, bacteroides spp. and mobiluncus curtisii) was performed in women with antenatal foetus death. specimens from forty women with this pathology were studied. control group consisted of 100 pregnant women of the adequate age groups and gestation periods, but with live foetus. vaginal smears treated by the use of polymerase chain reac ... | 2006 | 17057305 |
| lactobacillus strains isolated from the vaginal microbiota of healthy women inhibit prevotella bivia and gardnerella vaginalis in coculture and cell culture. | the purpose of this study was to investigate how human vaginal isolates of lactobacillus acidophilus, lactobacillus jensenii, lactobacillus gasseri and lactobacillus crispatus inhibit the vaginosis-associated pathogens gardnerella vaginalis and prevotella bivia. results show that all the strains in coculture condition reduced the viability of g. vaginalis and p. bivia, but with differing degrees of efficacy. the treatment of g. vaginalis- and p. bivia-infected cultured human cervix epithelial he ... | 2006 | 17059467 |
| the international infections in pregnancy (iip) study: variations in the prevalence of bacterial vaginosis and distribution of morphotypes in vaginal smears among pregnant women. | the objective of the study was to determine the prevalence of bacterial vaginosis and the distribution of associated morphotypes among asymptomatic pregnant women in different countries. | 2006 | 17074543 |
| use of dna hybridization test for diagnosing bacterial vaginosis in women with symptoms suggestive of infection. | the purpose of this study was to evaluate a dna hybridization test (affirm vpiii) as an alternative to gram stain for the rapid diagnosis of bacterial vaginosis in women with clinical signs of vaginal infection. vaginal specimens were collected from 321 symptomatic women, and analyzed for bacterial vaginosis by both gram stain using nugent criteria and dna hybridization test. sensitivity, specificity, positive predictive value, and negative predictive value of the dna hybridization test were det ... | 2006 | 17078859 |
| [causation and treatment of infectious leucorrhoea at the cocody university hospital (abidjan, côte d'ivoire)]. | to determine the frequency of different bacteria causing infectious leucorrhoea and to calculate the percentage of cures after standard treatment. | 2006 | 17284396 |
| interaction of erythrocytes with clue cells: light microscopic observation. | 2006 | 16355398 | |
| etiologies of nongonococcal urethritis: bacteria, viruses, and the association with orogenital exposure. | the purpose of the present study was to determine pathogens and behaviors associated with nongonococcal urethritis (ngu) and the usefulness of the urethral smear in predicting the presence of pathogens. | 2006 | 16388480 |
| throwing the dice for the diagnosis of vaginal complaints? | vaginitis is among the most common conditions women are seeking medical care for. although these infections can easily be treated, the relapse rate is high. this may be due to inadequate use of the diagnostic potential. | 2006 | 16503990 |
| antibiotic susceptibility of atopobium vaginae. | previous studies have indicated that a recently described anaerobic bacterium, atopobium vaginae is associated with bacterial vaginosis (bv). thus far the four isolates of this fastidious micro-organism were found to be highly resistant to metronidazole and susceptible for clindamycin, two antibiotics preferred for the treatment of bv. | 2006 | 16542416 |
| [prevalence of the hiv infection and five other sexually-transmitted infections among sex workers in niamey, niger]. | we performed a systematic screening of hiv infection and five other sexually transmitted infections on a consecutive sample of 529 female sex workers from niamey. hiv seroprevalence rate was 30.1%, with hiv-1 profile in 93.1% of the cases. for curable sti, 4.7% of women had treponematosis, 7% gonorrhoea, 16.8% gardnerella vaginalis, 36.9% mycoplasma hominis and 68.2% chlamydia trachomatis infection. the percentage of women with at least one sti was significantly higher in hiv positive's: 95.6% a ... | 2006 | 16568677 |
| prevalence of gardnerella vaginalis and atopobium vaginae in virginal women. | the objectives of this study was to determine the prevalence of gardnerella vaginalis and atopobium vaginae in virginal women and to establish if nonpenetrative sexual activity is associated with these organisms. | 2006 | 16601662 |
| inhibition of gardnerella vaginalis by lactobacilli. | 2006 | 16603163 | |
| probiotics for women's health. | the goals of this research were 2-fold: (1) to determine whether a commercially available probiotic mixture (vsl-3) could survive and grow in a continuous culture system simulating the vaginal environment and (2) to determine whether the probiotic mixture was capable of suppressing the growth of a known vaginal vault pathogen, gardnerella vaginalis. | 2006 | 16633132 |
| variation in vaginal immune parameters and microbial hydrolytic enzymes in bacterial vaginosis positive pregnant women with and without mobiluncus species. | this study was undertaken to assess if levels of interleukin-1beta (il-1beta), il-8, sialidase, prolidase and immunoglobulin a against gardenerella vaginalis hemolysin (anti-gvh iga) in vaginal secretions differ between bv+ women with (m+) and without (m-) mobiluncus spp. | 2006 | 16643824 |
| treatment and management of bacterial vaginosis in pregnancy: current and future perspectives. | bacterial vaginosis accounts for the majority of cases of vaginal discharge and has been consistently linked to an increased risk of preterm delivery. bacterial vaginosis is characterized by the reduced number or absence of hydrogen peroxide-producing lactobacillus spp., which promotes the overgrowth of anaerobic bacteria, including gardnerella vaginalis, mycoplasma hominis, bacteroides spp., and mobiluncus spp. black race, higher-risk sexual activity, frequent vaginal douching and the substanti ... | 2006 | 19803898 |
| quantitative determination by real-time pcr of four vaginal lactobacillus species, gardnerella vaginalis and atopobium vaginae indicates an inverse relationship between l. gasseri and l. iners. | most studies of the vaginal microflora have been based on culture or on qualitative molecular techniques. here we applied existing real-time pcr formats for lactobacillus crispatus, l. gasseri and gardnerella vaginalis and developed new formats for atopobium vaginae, l. iners and l. jensenii to obtain a quantitative non culture-based determination of these species in 71 vaginal samples from 32 pregnant and 28 non-pregnant women aged between 18 and 45 years. | 2007 | 18093311 |
| modulation of vaginal immune response among pregnant women with bacterial vaginosis by trichomonas vaginalis, chlamydia trachomatis, neisseria gonorrhoeae, and yeast. | this study was undertaken to examine the influence of coinfections on vaginal innate and adaptive immunity, and microbial enzyme activities of pregnant women with bacterial vaginosis (bv). | 2007 | 17306653 |
| the role of gene-environment interaction in predicting adverse pregnancy outcome. | this article focuses on the influence of gene-environment interaction on pregnancy outcome. in particular, we focus on those adverse outcomes related to subclinical infection and the resultant inflammation of gestational tissues. we identify genetic association studies on pregnancy-related disorders with an infectious/inflammatory etiology. all studies in this field have focused on spontaneous preterm delivery and/or preterm and premature rupture of membranes. we discuss those articles where an ... | 2007 | 17369098 |
| [progress in the studies of gardnerella vaginal]. | gardnerella vaginal is one of the main pathogenic floras of female bacterial vaginosis. sexually transmitted, it causes fallopian pregnancy, premature rupture of the foetal membrane, neonate premature delivery and other bad pregnancy outcome. gardnerella vaginal is also involved in male urogenital tract infection. this review summarizes the recent advances in the studies of gardnerella vaginal in such aspects as its categorization, biocharacteristics, biogrouping, epidemiology, pathogenesis, dia ... | 2007 | 17393790 |
| effect of lactobacillus challenge on gardnerella vaginalis biofilms. | bacterial vaginosis (bv) is the most common infectious condition in women. it is caused primarily by anaerobic bacteria which rapidly form biofilms recalcitrant to antibiotic treatment, elevate vaginal ph, induce inflammatory processes and displace indigenous lactobacilli from the vault. gardnerella vaginalis is commonly associated with these infections. microscopy analysis showed that within 72 h, viable g. vaginalis covered a surface area of 567 microm(2), reached a depth of 16 microm and a de ... | 2007 | 17234391 |
| an altered immunity hypothesis for the development of symptomatic bacterial vaginosis. | the hypothesis is advanced that the transition from a lactobacillus-dominated vaginal microflora to a microflora characteristic of bacterial vaginosis (bv), as well as development of the adverse consequences of bv in some women but not in others, are due to alterations in innate immunity. a microbial-induced inhibition of toll-like receptor expression and/or activity may block induction of proinflammatory immunity and lead to the proliferation of atypical vaginal bacteria. a lack of 70-kda heat- ... | 2007 | 17243059 |
| symptomatic candidiasis: using self sampled vaginal smears to establish the presence of candida, lactobacilli, and gardnerella vaginalis. | in a prospective cohort study, 10 symptomatic women with recurrent vulvovaginal candidiasis were taught how to prepare vaginal smears of their own vaginal fluids on days 7, 14, 21, and 28. the 40 smears were stained with the pas-method and examined by three different cytopathologists for presence of candida. thereafter, the smears were restained with giemsa-stain to determine presence of lactobacilli, gardnerella vaginalis ("clue cells") and neutrophils. all three cytopathologists unequivocally ... | 2007 | 17854078 |
| bacterial colonization in suspected sexually abused children. | previous studies concluded that symptomatic prepubertal children with gardnerella vaginalis infection should be investigated for child sexual abuse. gardnerella vaginalis is only one out of a group of organism that cause bacterial vaginosis. the aim of the study was to report the frequency of bacterial colonization as well as sexually transmitted diseases and to correlate these data with patient characteristics. | 2007 | 17868895 |
| clinical trial of interconceptional antibiotics to prevent preterm birth: subgroup analyses and possible adverse antibiotic-microbial interaction. | the purpose of this study was to explore whether endometrial microbial colonization and plasma cell endometritis are risk factors for adverse pregnancy outcomes, and whether these outcomes are influenced by interactions between interconceptional antibiotics and the micro-flora. | 2007 | 17904962 |
| [streptococcus group b--association with aerobic vaginitis and ability to human cell lines activation]. | the aim of this study was to estimate: the frequency of aerobic vaginitis, susceptibility of the gbs isolated from vagina of non-pregnant women with and without cervicitis to selected antibiotics and chemotherapeutics and the proinflammatory cytokines production by hela, thp-i, u - 937 cells after stimulation by vaginal gbs. our results indicated low frequency of the aerobic vaginitis -4.5% among non-pregnant young women and ability of the vaginal gbs to release proinflammatory cytokines by huma ... | 2007 | 17929406 |
| [biotypes and antibiotic resistance patterns of gardnerella vaginalis strains isolated from healthy women and women with bacterial vaginosis]. | as gardnerella vaginalis is accepted as a member of normal vaginal flora, it is one of the dominant species which has been related to bacterial vaginosis (bv). the aim of this study was to determine the isolation rate, biotypes and antibiotic resistance patterns of g.vaginalis from the vaginal swab samples of 408 women who were admitted to the outpatient clinics of family planning center. hippurate hydrolysis, lipase and beta-galactosidase tests were performed for biotyping the isolates, and aga ... | 2007 | 17427549 |
| rapid characterization of the normal and disturbed vaginal microbiota by application of 16s rrna gene terminal rflp fingerprinting. | bacterial vaginosis (bv) is a prevalent infection in women of reproductive age associated with numerous sequelae, including preterm delivery, amniotic fluid infections and an increased risk of acquiring sexually transmitted diseases. the vaginal microbiota in bv patients is characterized by a shift from lactobacilli to a diverse spectrum of mostly anaerobic bacteria. in this study, terminal restriction fragment length polymorphism (t-rflp) was used to characterize the vaginal bacterial communiti ... | 2007 | 17510259 |
| [frequency of gardnerella vaginalis in vaginal smears of hysterectomized women]. | this work intended to evaluate the frequency of gardnerella vaginallis in cervicalvaginal smears of hysterectomized women when compared to smears of non-hysterectomized women. | 2007 | 17568922 |
| [conjunctivitis due to gardnerella vaginalis]. | 2007 | 17583660 | |
| [the bacterial vaginosis--treatment problems]. | the changes in vaginal ecosystem (decreasing number of h2o2 producing lactobacillus spp. and increasing number of mobiluncus spp., gardnerella vaginalis, atopobium vaginae, prevotella spp., mycoplasma hominis, peptostreptococcus spp.) appears to be a major cause of bacterial vaginosis (bv). bacterial vaginosis is associated with infectious complications in pregnant and non-pregnant women and increasing risk of sti. the preventive role of natural vaginal microflora and problems of bv treatment ar ... | 2007 | 17607971 |
| alloscardovia omnicolens gen. nov., sp. nov., from human clinical samples. | the taxonomic position of 12 isolates tentatively assigned to the genus bifidobacterium on the basis of a limited phenotypic characterization was examined. the isolates were collected between 1978 and 2005 in belgium, sweden and norway, and originated from various human clinical samples, including urine, blood, urethra, oral cavity, tonsil, and abscesses of lung and aortic valve. on the basis of band number and clustering analysis, repetitive dna element-based pcr fingerprinting using the boxa1r ... | 2007 | 17625172 |
| probiotics for the treatment of women with bacterial vaginosis. | this review considers whether probiotics are effective agents for the treatment and/or prevention of bacterial vaginosis (bv). there seems to be an association between the absence of, or low concentrations of, vaginal lactobacilli and the development of bv. many studies have suggested that the presence of h2o2-producing vaginal lactobacilli may protect against bv, although some studies do not support this hypothesis. in-vitro studies have suggested that certain specific strains of lactobacilli a ... | 2007 | 17633390 |
| targeted pcr for detection of vaginal bacteria associated with bacterial vaginosis. | several novel bacterial species have been detected in subjects with bacterial vaginosis (bv) by using broad-range pcr assays, but this approach is insensitive for detecting minority species. we developed a series of taxon-directed 16s rrna gene pcr assays for more sensitive detection of key vaginal bacteria. we sought to determine the prevalence of each species in the vagina, its association with bv, and the utility of pcr for the microbiological diagnosis of bv. targeted pcr assays were develop ... | 2007 | 17687006 |
| effect of biofilm phenotype on resistance of gardnerella vaginalis to hydrogen peroxide and lactic acid. | bacterial vaginosis is the most common vaginal disorder worldwide. certain lactobacilli produce h2o2 and lactic acid, which normally suppress growth of anaerobes; however, in bacterial vaginosis, gardnerella vaginalis and other anaerobes proliferate, and the number of lactobacilli decreases. g. vaginalis colonizes the vaginal epithelium as a biofilm, which likely plays a role in colonization and relapsing infection. | 2007 | 17689638 |
| altered expression of adhesion molecules in inflammatory cervical smears. | the aim of the present study was to evaluate the expression of pan-cadherin and beta-catenin in cervical smears with various types of infectious agents. | 2008 | 17573909 |
| isolation of the bacillus subtilis antimicrobial peptide subtilosin from the dairy product-derived bacillus amyloliquefaciens. | to purify and characterize an antimicrobial protein (bacteriocin) isolated from the dairy product-derived bacillus amyloliquefaciens. | 2008 | 17976171 |
| an adherent gardnerella vaginalis biofilm persists on the vaginal epithelium after standard therapy with oral metronidazole. | the purpose of this study was to determine the efficacy of standard treatment with oral metronidazole in the eradication of the bacterial vaginosis biofilm. | 2008 | 18005928 |
| gardnerella vaginalis bacteremia in a previously healthy man: case report and characterization of the isolate. | gardnerella vaginalis in women causes vaginitis or infections in other sites, such as the urinary tract, but is an infrequent cause of bacteremia. bacteremia in men is very rare and is typically associated with immunocompromised states. here we describe g. vaginalis bacteremia in a previously healthy man with renal calculi and urosepsis. | 2008 | 18057138 |
| rapid detection of atopobium vaginae and association with organisms implicated in bacterial vaginosis. | atopobium vaginae, a fastidious, anaerobic, gram-positive cocci-shaped bacterium that generates large quantities of lactic acid, is associated with bacterial vaginosis (bv). published nucleic acid amplification tests for identifying a. vaginae are directed toward the 16s ribosomal dna with suboptimal specificity and require isolation of the organism. here, sequencing of an a. vaginae genomic library has led to the development of a highly specific and sensitive real-time pcr test for detection of ... | 2008 | 17913456 |
| vaginal il-8 levels are positively associated with candida albicans and inversely with lactobacilli in hiv-infected women. | il-8/cxcl8 is induced during infections, but has not been reported for candida albicans colonization of the female genital tract. cervicovaginal lavage (cvl) samples were collected from 406 hiv-infected women. il-8 levels were evaluated by elisa and compared with levels of c. albicans detected by potassium hydroxide (koh) and pcr. levels of lactobacilli, gardnerella vaginalis and mycoplasma hominis were also determined by pcr. il-8 was significantly higher in samples from women with candida, and ... | 2008 | 18243333 |
| placental histological inflammation and reproductive tract infections in a low risk pregnant population in latvia. | to investigate the correlation of reproductive tract infections (rti) and endogenous vaginal flora at first antenatal consultation with placental histological inflammation. | 2008 | 18307078 |
| erythema multiforme associated with gardnerella vaginosis and elevated circulating cxcr3+ b cells. | 2008 | 18312462 | |
| [protease and phospholipase activities of candida albicans isolated from vaginal secretions with different ph values]. | even though vulvovaginal candidiasis and bacterial vaginosis are seldom simultaneously found, we have detected this association at an above average frequency. thus, we set out to study the activity of proteinases and phospholipases, virulence factors of candida albicans, to assess their role in the above mentioned association. of a total of 70 candida isolates were retrieved from samples of vaginal secretions analyzed at our diagnostic service, 65 were identified as c. albicans (a group of n=26 ... | 2008 | 18338921 |
| a retrospective analysis of isolates from patients with vaginitis in a private greek obstetric/gynecological hospital (2003-2006). | vaginitis is a common cause of complaints in women in various parts of the world. through our literature search, we identified noteworthy differences in the etiology of vaginitis based on the geographic location. this study was conducted to describe microbial etiology in a population of symptomatic women with vaginitis in greece. | 2008 | 18376352 |
| functional and phylogenetic characterization of vaginolysin, the human-specific cytolysin from gardnerella vaginalis. | pore-forming toxins are essential to the virulence of a wide variety of pathogenic bacteria. gardnerella vaginalis is a bacterial species associated with bacterial vaginosis (bv) and its significant adverse sequelae, including preterm birth and acquisition of human immunodeficiency virus. g. vaginalis makes a protein toxin that generates host immune responses and has been hypothesized to be involved in the pathogenesis of bv. we demonstrate that g. vaginalis produces a toxin (vaginolysin [vly]) ... | 2008 | 18390664 |
| the lower genital tract microbiota in relation to cytokine-, slpi- and endotoxin levels: application of checkerboard dna-dna hybridization (cdh). | in the present study the lower genital tract microbiota in asymptomatic fertile women (n=34) was identified and quantified by culturing vaginal secretions. also, vaginal and cervical samples were analyzed by a semiquantitative checkerboard dna-dna hybridization technique (cdh) based on genomic probes prepared from 13 bacterial species (bacteroides ureolyticus, escherichia coli, fusobacterium nucleatum, gardnerella vaginalis, mobiluncus curtisii ss curtisii, prevotella bivia, prevotella disiens, ... | 2008 | 18397461 |
| the polymicrobial hypothesis of bacterial vaginosis causation: a reassessment. | the cause of bacterial vaginosis remains controversial. the two leading hypotheses are that gardnerella vaginalis is the specific aetiologic agent versus the polymicrobial hypothesis that g. vaginalis acts in concert with other bacteria, principally anaerobes, to produce the disease. here we reassess the prevailing polymicrobial hypothesis, finding it conceptually problematical. no host initiating factors as postulated have been identified, nor is there conclusive evidence that g. vaginalis lack ... | 2008 | 18397551 |
| atopobium vaginae triggers an innate immune response in an in vitro model of bacterial vaginosis. | bacterial vaginosis is the most common vaginal disorder among women of reproductive age. the pathogenesis of bacterial vaginosis is poorly understood, but is defined by a transition in the vaginal flora from the predominant lactobacillus species to other bacterial species such as atopobium vaginae and gardnerella vaginalis. this change is associated with an increase in vaginal cytokine secretion. we hypothesize that vaginal epithelial cells respond to bacterial vaginosis-associated bacteria by t ... | 2008 | 18403235 |
| gardnerella vaginalis and lactobacillus sp in liquid-based cervical samples in healthy and disturbed vaginal flora using cultivation-independent methods. | our objective was to determine the morphotype of the adherent bacteria in liquid-based cytology (lbc) in smears with healthy and disturbed vaginal flora. and to use pcr technology on the same fixed cell sample to establish dna patterns of the 16s rna genes of the bacteria in the sample. thirty samples were randomly selected from a large group of cervical cell samples suspended in a commercial coagulant fixative "(boonfix)." pcr was used to amplify dna of five bacterial species: lactobacillus aci ... | 2008 | 18418885 |
| antibiotic resistance of gardnerella vaginalis in recurrent bacterial vaginosis. | fifty strains of gardnerella vaginalis isolated from 321 high vaginal swabs over a period of five months were tested for their antibiotic sensitivity. sixty eight per cent of all isolates were resistant to metronidazole while 76% were sensitive to clindamycin. all the strains isolated from cases with recurrence of infection were resistant to metronidazole. clindamycin therapy has a better clinical efficacy than metronidazole in cases of recurrent bacterial vaginosis. | 2008 | 18445953 |
| frequency of infectious agents for vaginitis in patients with a cytological diagnosis of atypical squamous cells of undetermined significance. | to evaluate the presence of infectious agents for vaginitis in patients with ascus. | 2008 | 18459549 |
| molecular quantification of gardnerella vaginalis and atopobium vaginae loads to predict bacterial vaginosis. | bacterial vaginosis (bv) is a poorly detected public health problem that is associated with preterm delivery and for which no reliable diagnostic tool exists. | 2008 | 18513147 |
| cytologically diagnosed gardnerella vaginalis infection and cervical (pre)neoplasia as established in population-based cervical screening. | cervical inflammation has been proposed as a cofactor in the development of cervical cancer. the purpose of this study was to document the prevalence of cervical (pre)neoplastic changes in asymptomatic women with a cytologically diagnosed gardnerella vaginalis infection. | 2008 | 18565490 |
| quantitative survival of aerobic and anaerobic microorganisms in port-a-cul and copan transport systems. | transport media should preserve the viability and stability of microorganisms in clinical specimens. in this study, the port-a-cul transport system and the copan transport system without charcoal, both designed to preserve anaerobes, were evaluated. dacron swabs were inoculated with two combinations of facultative and anaerobic organisms typically found in vaginal swab samples. combination i contained candida albicans, escherichia coli, enterococcus spp., group b streptococci, lactobacillus cris ... | 2008 | 18579722 |
| efficacy and tolerance of metronidazole and miconazole nitrate in treatment of vaginitis. | to evaluate the efficacy and tolerability of a vaginal pessary containing 750 mg of metronidazole and 200 mg of miconazole nitrate used daily for 7 days in the treatment of vaginitis. | 2008 | 18603245 |
| bacterial vaginosis: a sexually enhanced disease. | 2008 | 18663053 | |
| reassessment of bv causation. | 2008 | 18725563 | |
| bacterial vaginosis presence in sexually active women in tuzla canton area. | the goal of our research was to determine the presence of bacterial vaginosis in sexually active women in tuzla canton area. diagnosis determination for bacterial vaginosis was conducted on the basis of three out of four internationally accepted criteria according to amsel and isolation and identification of gardnerella vaginalis (g. vaginalis) by standard microbiological procedures. bacterial vaginosis was diagnosed in 20,5 % (41/200) women who asked for gynaecologist's help due to their person ... | 2008 | 19125702 |
| clinical evaluation of affirm vp iii in the detection and identification of bacterial vaginosis. | 2008 | 19129080 | |
| importance of isolation and biotypization of gardnerella vaginalis in diagnosis of bacterial vaginosis. | the natural habitat of gardnerella vaginalis is a vagina since it could be located among 69% of women who have no signs of vaginal infection and in the vagina of as many as 13.5% girls. g. vaginalis is almost certainly identified among women diagnosed with bacterial vaginosis as well as in the urethra of their sexual partner. the increase in prevalence and concentration of g. vaginalis among patients diagnosed with this syndrome confirms that g. vaginalis plays a significant role in its pathogen ... | 2008 | 18816262 |
| prevotella bivia as a source of lipopolysaccharide in the vagina. | to compare vaginal lipopolysaccharides (lps) concentrations between patients with and without bacterial vaginosis (bv), to evaluate the correlation between prevotella bivia colonization density and lps concentration, and to determine the impact of lps on loss of dopamine neurons (da). | 2008 | 18849004 |
| spermicidal activity of the safe natural antimicrobial peptide subtilosin. | bacterial vaginosis (bv), a condition affecting millions of women each year, is primarily caused by the gram-variable organism gardnerella vaginalis. a number of organisms associated with bv cases have been reported to develop multidrug resistance, leading to the need for alternative therapies. previously, we reported the antimicrobial peptide subtilosin has proven antimicrobial activity against g. vaginalis, but not against the tested healthy vaginal microbiota of lactobacilli. after conducting ... | 2008 | 18923673 |
| longitudinal trajectory of bacterial vaginosis during pregnancy. | this study was undertaken to characterize the course of bacterial vaginosis in pregnancy and to discern the bacterial morphotypes responsible for infection. | 2008 | 18928996 |
| what's the treatment for bacterial vaginosis? | a 28-weeks-pregnant patient complained of vaginal irritation and a malodorous white discharge that didn't improve after she used an over-the-counter cream indicated for yeast infections. what's a more effective treatment? | 2008 | 19033972 |
| natural antimicrobials and their role in vaginal health: a short review. | lactobacillus species maintain the vaginal ecosystem in a healthy condition by production of antimicrobial substances. depletion of lactobacilli in the vagina results in bacterial vaginosis (bv), where the normal flora is replaced by several bacterial pathogens, usually gardnerella vaginalis and obligate anaerobes. bv may cause complications such as premature labor, low birth weight and increased risk of hiv acquisition. the currently recommended antibiotic treatments for bv are not always effec ... | 2008 | 20657710 |
| gardnerella infection as distinguished from cervical dysbacteriosis: the advantageous spin-off of cervical screening. | to evaluate cytologic diagnoses of dysbacteriosis and gardnerella infection and to obtain insight into the diagnostic problems of gardnerella. | 2009 | 19697722 |
| multiplex detection of bacteria associated with normal microbiota and with bacterial vaginosis in vaginal swabs by use of oligonucleotide-coupled fluorescent microspheres. | bacterial vaginosis (bv) is a recurrent condition that is associated with a range of negative outcomes, including the acquisition of human immunodeficiency virus and other sexually transmitted diseases, preterm births, and pelvic inflammatory disease. in contrast to the lactobacillus-dominated normal vaginal microbiota, bv is characterized by a lack of lactobacilli and an abundance of anaerobic and gram-negative organisms, including gardnerella vaginalis and atopobium vaginae. to date, the labor ... | 2009 | 19794034 |
| [vaginal ectopia of urethra as a cause of chronic recurrent cystitis in women: diagnosis and treatment]. | ectopia of the urethral opening (female hypospadia) often causes chronic recurrent cystitis in women because of a retrograde delivery of urogenital infection from the introitus and vagina to the short wide urethra and urinary bladder. etiologically, cystitis develops due to pathogenic vaginal microflora: chlamydia trachomatis, mycoplasma genitalium, ureaplasma, urealyticum, gardnerella vaginalis, candida, e. coli and other gram-negative bacteria. in 64 female patients aged 16-57 years with femal ... | 2009 | 20209864 |
| accuracy of the clinical diagnosis of vaginitis compared with a dna probe laboratory standard. | to estimate the accuracy of the clinical diagnosis of the three most common causes of acute vulvovaginal symptoms (bacterial vaginosis, candidiasis vaginitis, and trichomoniasis vaginalis) using a traditional, standardized clinical diagnostic protocol compared with a dna probe laboratory standard. | 2009 | 19104364 |
| colonization of gardnerella vaginalis in semen of infertile men: prevalence, influence on sperm characteristics, relationship with leukocyte concentration and clinical significance. | to determine the prevalence of gardnerella vaginalis in semen from infertile men and their negative effects on semen quality. | 2009 | 19602896 |
| utility of reflexive semen cultures for detecting bacterial infections in patients with infertility and leukocytospermia. | this study evaluated the benefit of reflexive semen cultures in peroxidase-positive samples drawn from 17,142 consecutive samples analyzed, in which 4.7% (807/17,142) had leukocytospermia and 12.7% of those (103/807) grew significant bacterial colonies on culture. considering the potential ramifications of the use of samples with bacteriospermia, we conclude that reflexive culture results are cost-effective and prudent. | 2009 | 18980764 |
| preterm labor and bacterial vaginosis-associated bacteria among urban women. | bacterial vaginosis (bv) affects millions of women, is extremely prevalent and is frequently chronic. we recognize numerous microbiologic variations among women with bv and this variability may explain the limited effectiveness of metronidazole in curing bv and/or reducing the risk of spontaneous preterm birth (sptb) among bv-positive pregnant women. we assessed the independent role of seven common bv-associated bacteria on the risk of spontaneous preterm birth (sptb) among urban pregnant women. | 2009 | 18999913 |