Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| comparison of the fuel oil biodegradation potential of hydrocarbon-assimilating microorganisms isolated from a temperate agricultural soil. | strains of hydrocarbon-degrading microorganisms (bacteria and fungi) were isolated from an agricultural soil in france. in a field, a portion was treated with oily cuttings resulting from the drilling of an onshore well. the cuttings which were spread at the rate of 600 g hc m-2 contained 10% of fuel oil hydrocarbons (hc). another part of the field was left untreated. three months after hc spreading, hc adapted bacteria and fungi were isolated at different soil depths in the two plots and identi ... | 1999 | 10231986 |
| movement disorders in encephalitis induced by rhodococcus aurantiacus infection relieved by the administration of l-dopa and anti-t-cell antibodies. | mice injected with rhodococcus aurantiacus by the intravenous (i.v.) route show neurological disorders, hemiparesis, vertical headshake and turn-round gait after day 7 postinfection (p.i.). neurological symptoms caused by i.v. inoculation of r. aurantiacus were relieved by treatment with levodopa (l-dopa). r. aurantiacus was isolated from the brain and was found to be completely eliminated at day 7 p. i. focal encephalitis was mainly observed in the brain stem, and t cells could be isolated from ... | 1999 | 10233672 |
| tumour necrosis factor and interferon-gamma are required in host resistance against virulent rhodococcus equi infection in mice: cytokine production depends on the virulence levels of r. equi. | rhodococcus equi is a facultative intracellular bacterial pathogen that causes pneumonia in foals and immunosuppressed humans. there are at least three virulence levels of r. equi and these pathogenicities are associated, in mice, with the presence of virulence plasmids. this study focused on cytokine secretion, in mice, in the course of a primary infection with sublethal doses of r. equi strains of different virulence levels (virulent, intermediately virulent and avirulent). tumour necrosis fac ... | 1999 | 10233686 |
| distribution of a nocardia brasiliensis catalase gene fragment in members of the genera nocardia, gordona, and rhodococcus. | an immunodominant protein from nocardia brasiliensis, p61, was subjected to amino-terminal and internal sequence analysis. three sequences of 22, 17, and 38 residues, respectively, were obtained and compared with the protein database from genbank by using the blast system. the sequences showed homology to some eukaryotic catalases and to a bromoperoxidase-catalase from streptomyces violaceus. its identity as a catalase was confirmed by analysis of its enzymatic activity on h2o2 and by a double-s ... | 1999 | 10325357 |
| production of cholesterol oxidase by rhodococcus equi no. 23 in a jar fermenter. | rhodococcus equi no. 23 was grown in a batch fermenter. the effects of cultivation temperature, ph of the culture medium, aeration rate and agitation speed on the production of cholesterol oxidase (cholox) by the test organism were examined. results revealed that the cultivation temperature, the ph of the medium, the aeration rate and the agitation speed all affected the production of cholox by r. equi no. 23. adjusting the operation variables during the cultivation period increased the producti ... | 1999 | 10334951 |
| live virulent rhodococcus equi, rather than killed or avirulent, elicits protective immunity to r. equi infection in mice. | mice inoculated intravenously with a sublethal dose of live virulent rhodococcus equi atcc 33701 that contained an 85-kb virulence plasmid were immune to a lethal intravenous challenge of atcc 33701. this immunity depended upon the dose of immunization and developed rapidly: mice primed with 10(5) live atcc 33701 eliminated the challenged bacteria more rapidly than mice primed with doses ranging from 10(2) to 10(4) bacteria, and mice given 10(5) live atcc 33701 intravenously withstood the lethal ... | 1999 | 10340706 |
| infection by rhodococcus equi in a patient with aids: histological appearance mimicking whipple's disease and mycobacterium avium-intracellulare infection. | rhodococcus equi pneumonia with systemic dissemination is being reported increasingly in immunocompromised patients. this is the first case report of disseminated r equi infection with biopsy documented involvement of the large intestine. the patient was a 46 year old male with aids who was diagnosed with cavitating pneumonia involving the left lower lobe. r equi was isolated in culture from the blood and lung biopsies. subsequently, the patient developed anaemia, diarrhoea, and occult blood in ... | 1999 | 10343616 |
| transcriptional analysis of the nitrile-degrading operon from rhodococcus sp. acv2 and high level production of recombinant amidase with an escherichia coli-t7 expression system. | northern blotting analysis with rna probes derived from amidase and nitrile hydratase genes from rhodococcus sp. acv2 revealed that both genes are part of the same operon. rnase protection mapping and sequence analysis indicated that the operon is probably under the control of a sigma 70-like promoter located upstream from the amidase gene. plasmids were constructed with the cloned genes under tac and lac promoter control. expression of amda was demonstrated in escherichia coli. in another const ... | 1999 | 10347869 |
| actinomycetes in karstic caves of northern spain (altamira and tito bustillo). | a variety of isolation procedures were carried out to study the involvement of bacteria in the colonisation and biodeterioration of spanish caves with paleolithic rock art (altamira and tito bustillo). the applied techniques mainly aimed to isolate heterotrophic bacteria such as streptomycetes, nocardioform and coryneform actinomycetes, and other gram-positive and gram-negative bacteria. the results demonstrated that actinomycetes were the most abundant gram-positive bacteria in the caves. actin ... | 1999 | 10353805 |
| an enzyme controlled by light: the molecular mechanism of photoreactivity in nitrile hydratase. | extensive studies have revealed the molecular mechanism of the photoreactivity of nitrile hydratase from rhodococcus sp. n-771. in the inactive enzyme, nitric oxide is bound to the non-heme ferric iron at the catalytic center, stabilized by a claw-like structure formed by two post-translationally modified cysteines and a serine. the inactive nitrile hydratase is activated by the photoinduced release of the nitric oxide. this result might provide a means of designing novel photoreactive chemical ... | 1999 | 10354562 |
| peripheral blood lymphocyte subpopulations and immunoglobulin concentrations in healthy foals and foals with rhodococcus equi pneumonia. | infectious diseases are common in foals aged 1-5 months. the objectives of this investigation were to evaluate immunologic parameters in foals from birth to weaning to establish reference values for the proportion of circulating lymphocytes that were helper (cd4+) or cytotoxic (cd8+) t cells, or b cells; to measure serum immunoglobulin (igm and igg) concentrations; and to compare these immunologic parameters to values in foals with naturally occurring rhodococcus equi pneumonia and in adult hors ... | 1999 | 10357110 |
| linear alkanesulfonates as carbon and energy sources for gram-positive and gram-negative bacteria. | several bacteria from soil and rainwater samples were enriched and isolated with propanesulfonate or butanesulfonate as sole carbon and energy source. most of the strains isolated utilized nonsubstituted alkanesulfonates with a chain length of c3-c6 and the substituted sulfonates taurine and isethionate as carbon and energy source. a gram-positive isolate, p40, and a gram-negative isolate, p53, were characterized in more detail. phylogenetic analysis grouped strain p40 within group iv of the gen ... | 1999 | 10369899 |
| role of the 85-kilobase plasmid and plasmid-encoded virulence-associated protein a in intracellular survival and virulence of rhodococcus equi. | rhodococcus equi is a facultative intracellular pathogen of macrophages and a cause of pneumonia in young horses (foals) and immunocompromised people. isolates of r. equi from pneumonic foals typically contain large, 85- or 90-kb plasmids encoding a highly immunogenic virulence-associated protein (vapa). the objective of this study was to determine the role of the 85-kb plasmid and vapa in the intracellular survival and virulence of r. equi. clinical isolates containing the plasmid and expressin ... | 1999 | 10377138 |
| the glu residue in the conserved asn-glu-pro sequence of endoglycoceramidase is essential for enzymatic activity. | endoglycoceramidase (egcase) is an enzyme capable of cleaving the glycosidic linkage between oligosaccharides and ceramides of various glycosphingolipids. we previously cloned the gene encoding egcase ii of rhodococcus sp. m-777 and reported that the deduced amino acid sequence contained the asn-glu-pro (nep) sequence, conserved as part of the active site of family a cellulases (endo-1,4-beta-glucanases) (j. biol. chem. 272, 19846, 1997). the nep sequence was also found in the deduced amino acid ... | 1999 | 10381348 |
| effect of gamma radiation on native endolithic microorganisms from a radioactive waste deposit site. | a time-course experiment was conducted to evaluate the effects of gamma radiation on the indigenous microbiota present in rock obtained from yucca mountain, nevada test site. microcosms were constructed by placing pulverized yucca mountain rock in polystyrene cylinders. continuous exposure (96 h) at a dose rate of 1.63 gy/min was used to mimic the near-field environment surrounding waste canisters. the expected maximum surface dose rate from one unbreached canister designed to contain spent nucl ... | 1999 | 10381842 |
| biodesulfurization. | microbial sulfur-specific transformations have been identified that selectively desulfurize organic sulfur compounds in fossil fuels. recent discoveries related to biodesulfurization mechanisms may lead to commercial applications of biodesulfurization through engineering recombinant strains for over-expression of biodesulfurization genes, removal of end product repression, and/or by combining relevant industrial and environmental traits with improvements in bioprocess design. | 1999 | 10383871 |
| physiological adaptations involved in alkane assimilation at a low temperature by rhodococcus sp. strain q15. | we examined physiological adaptations which allow the psychrotroph rhodococcus sp. strain q15 to assimilate alkanes at a low temperature (alkanes are contaminants which are generally insoluble and/or solid at low temperatures). during growth at 5 degrees c on hexadecane or diesel fuel, strain q15 produced a cell surface-associated biosurfactant(s) and, compared to glucose-acetate-grown cells, exhibited increased cell surface hydrophobicity. a transmission electron microscopy examination of strai ... | 1999 | 10388690 |
| a comparison between toxicity tests using single species and a microbial process. | in this study the sensitivity of the acetate mineralization process performed by five strains of microorganisms in soil for the toxicants zn2+ or pcp was calculated from the sensitivity of the contributing species. the species used were a fungus (aspergillus niger cbs 121.49), an actinomycete (streptomyces lividans 66), two gram-negative pseudomonas putida strains (mt-2 and dsm 50026) and a gram-positive strain rhodococcus erythropolis a177. for zinc the ec10 of the process performed by the five ... | 1999 | 10390842 |
| the smcl gene of listeria ivanovii encodes a sphingomyelinase c that mediates bacterial escape from the phagocytic vacuole. | the ruminant pathogen listeria ivanovii differs from listeria monocytogenes in that it causes strong, bizonal haemolysis and a characteristic shovel-shaped co-operative haemolytic ('camp-like') reaction with rhodococcus equi. we cloned the gene responsible for the differential haemolytic properties of l. ivanovii, smcl. it encodes a sphingomyelinase c (smase) highly similar (> 50% identity) to the smases from staphylococcus aureus (beta-toxin), bacillus cereus and leptospira interrogans. smcl wa ... | 1999 | 10417642 |
| etiology, clinical features and outcome of splenic microabscesses in hiv-infected patients with prolonged fever. | a prospective study was conducted to determine the etiology, clinical features, and outcome in a series of 32 consecutively enrolled hiv-infected patients with prolonged fever in whom high resolution (7.5 mhz) sonography revealed multiple splenic microabscesses. conventional (3.5 mhz) sonography showed no splenic abnormalities in any patients. the diagnoses were: tuberculosis (14), visceral leishmaniasis (7), disseminated mycobacterium avium complex infection (5), salmonella spp. bacteremia (2), ... | 1999 | 10421038 |
| disseminated rhodococcus equi infection in two goats. | rhodococcus equi infection was diagnosed in two goats from the same herd. at necropsy, numerous caseating granulomas were disseminated throughout the liver, lungs, abdominal lymph nodes, medulla of right humerus, and the right fifth rib of goat no. 1, and the liver of goat no. 2. histopathologic examination confirmed the presence of multiple caseating granulomas in these organs. numerous gram-positive and giemsa-positive coccobacilli were identified within the cytoplasm of macrophages. aerobic b ... | 1999 | 10421101 |
| isolation and characterization of indene bioconversion genes from rhodococcus strain i24. | rhodococcus strain 124 is able to convert indene into indandiol via the actions of at least two dioxygenase systems and a putative monooxygenase system. we have identified a cosmid clone from 124 genomic dna that is able to confer the ability to convert indene to indandiol upon rhodococcus erythropolis sq1, a strain that normally can not convert or metabolize indene. hplc analysis reveals that the transformed sq1 strain produces cis-(1r,2s)-indandiol, suggesting that the cosmid clone encodes a n ... | 1999 | 10422226 |
| characterization of antarctic hydrocarbon-degrading bacteria capable of producing bioemulsifiers. | during screening for biosurfactant-producing, n-alkane-degrading marine bacteria, two heterotrophic bacterial strains were isolated from enriched mixed cultures, obtained from terra nova bay (ross sea, antarctica) by using aliphatic and artomatic hydrocarbons as the principal carbon source. these gram-positive, aerobic, cocci-shaped bacteria use a various number of organic compounds, including aliphatic hydrocarbons, volatile fatty acids, and biphenyl. during cultivation on n-alkanes as sole sou ... | 1999 | 10423744 |
| isolation and characterization of catechol 1,2-dioxygenases from rhodococcus rhodnii strain 135 and rhodococcus rhodochrous strain 89: comparison with analogous enzymes of the ordinary and modified ortho-cleavage pathways. | catechol 1,2-dioxygenases of the ordinary ortho-cleavage pathway have been isolated from strains rhodococcus rhodnii 135 and rhodococcus rhodochrous 89 grown on phenol as the sole source of carbon and energy. the activities of the catechol 1,2-dioxygenases with 3- and 4-methylpyrocatechols were 1.3-1.5 times higher than those with pyrocatechol. the rate of oxidation of 3-chloropyrocatechol catalyzed by both enzymes was 20% of the rate of oxidation of unsubstituted pyrocatechol. the enzymes are h ... | 1999 | 10424908 |
| bioconversion of pyrimidine by resting cells of quinoline-degrading bacteria. | nine quinoline-degrading bacterial strains were tested for their ability to hydroxylate pyrimidine. all strains converted pyrimidine to uracil via pyrimidine-4-one in a cometabolic process. quinoline 2-oxidoreductases (quinors) were the catalysts of fortuitous pyrimidine hydroxylation. whereas in most strains the activity of the quinor towards pyrimidine was very low compared to its activity towards quinoline, quinor in crude extracts from comamonas testosteroni 63 showed a specific activity of ... | 1999 | 10427712 |
| in vitro activities of polycationic peptides alone and in combination with clinically used antimicrobial agents against rhodococcus equi. | the in vitro activities of magainin ii, nisin, and ranalexin alone and in combination with other antimicrobial agents against six clinical isolates of rhodococcus equi were investigated by mic and time-kill studies. all isolates were more susceptible to nisin. a positive interaction was observed when the peptides were combined with ampicillin, ceftriaxone, rifabutin, rifampin, azithromycin, clarithromycin, and vancomycin. | 1999 | 10428947 |
| effect of a mixed culture on co-oxidation during the degradation of saturated hydrocarbon mixture. | two bacterial strains, pseudomonas aeruginosa k1 and rhodococcus equi p1, were used to degrade cyclo-alkanes (such as decalin) by a co-oxidation mechanism. both strains possessed the capacity to degrade a broad range of n-alkane mixtures (c7 to c28) within 24 h of incubation. strain p1 rapidly degraded 10 gl-1 pristane within 24 h of incubation (mu = 0.36 h-1 and yx/s = 0.6). the addition of hexadecane as a growth substrate (above 0.5%, v/v) resulted in complete degradation of 1% (v/v) decalin b ... | 1999 | 10432589 |
| tertiary and quaternary structures of photoreactive fe-type nitrile hydratase from rhodococcus sp. n-771: roles of hydration water molecules in stabilizing the structures and the structural origin of the substrate specificity of the enzyme. | the crystal structure analysis of the fe-type nitrile hydratase from rhodococcus sp. n-771 revealed the unique structure of the enzyme composed of the alpha- and beta-subunits and the unprecedented structure of the non-heme iron active center [nagashima, s., et al. (1998) nat. struct. biol. 5, 347-351]. a number of hydration water molecules were identified both in the interior and at the exterior of the enzyme. the study presented here investigated the roles of the hydration water molecules in s ... | 1999 | 10433695 |
| heterologous expression and characterization of the purified oxygenase component of rhodococcus globerulus p6 biphenyl dioxygenase and of chimeras derived from it. | in this work, we have purified the his-tagged oxygenase (ht-oxygenase) component of rhodococcus globerulus p6 biphenyl dioxygenase. the alpha or beta subunit of p6 oxygenase was exchanged with the corresponding subunit of pseudomonas sp. strain lb400 or of comamonas testosteroni b-356 to create new chimeras that were purified ht-proteins and designated ht-alpha(p6)beta(p6), ht-alpha(p6)beta(lb400), ht-alpha(p6)beta(b-356), ht-alpha(lb400)beta(p6), and ht-alpha(b-356)beta(p6). ht-alpha(p6)beta(p6 ... | 1999 | 10438748 |
| degradation of trichloroethene by a linear-plasmid-encoded alkene monooxygenase in rhodococcus corallinus (nocardia corallina) b-276. | rhodococcus corallinus (formerly nocardia corallina) b-276, isolated with propene as sole carbon and energy source, is able to oxidize trichloroethene (tce). glucose- or propene-grown r. corallinus b-276 cells exhibited no difference in tce degradation efficiency. tce degradation was found to be growth-phase-dependent and maximum rates were monitored with stationary-phase cells. k(m) and vmax values for tce degradation of r. corallinus b-276 grown in nutrient broth medium in the presence of gluc ... | 1999 | 10439411 |
| differential detection of key enzymes of polyaromatic-hydrocarbon-degrading bacteria using pcr and gene probes. | bacteria with ability to degrade polyaromatic hydrocarbons (pahs), isolated from wastewater and soil samples, were investigated for their taxonomic, physiological and genetic diversity. eighteen isolates able to metabolize naphthalene or phenanthrene as sole carbon source were taxonomically affiliated to different subclasses of the proteobacteria (sphingomonas spp., acidovorax spp., comamonas spp. and pseudomonas spp.) and to phyla of gram-positive bacteria with low and high dna g + c content (p ... | 1999 | 10439412 |
| cometabolic oxidation of phenanthrene to phenanthrene trans-9,10-dihydrodiol by mycobacterium strain s1 growing on anthracene in the presence of phenanthrene. | mycobacterium strain s1, originally described as rhodococcus strain s1 by chemotaxonomic criteria, was isolated by growth on anthracene, and is unable to use any of nine other polycyclic aromatic compounds as carbon source. metabolism of phenanthrene during growth on anthracene as sole carbon source results in the accumulation of traces of a dihydrodiol metabolite in the growth medium, which, by comparison with authentic standards, has been tentatively identified as phenanthrene trans-9,10-dihyd ... | 1999 | 10446712 |
| pulmonary malacoplakia associated with rhodococcus equi infection in patients with aids: case report and review. | 1999 | 10451186 | |
| requirement for a different hydrophobic moiety and reliable chromogenic substrate for endo-type glycosylceramidases. | a series of synthetic lactosides with aglycones that differed in length and structure were used to determine the substrate specificity of endo-type glycosylceramidases. endoglycoceramidases (egcase) from bacteria preferred lactosides with an acylamide structure over simple n-alkyl lactosides. while ceramide glycanase (cgase) from leech did not show preference. n -acylaminoethyl beta-lactosides and n -alkyl lactosides were substrates for both egcase and cgase, but n-acylaminobutyl beta-lactosides ... | 1999 | 10460837 |
| degradation of alicyclic molecules by rhodococcus ruber cd4. | the present work describes investigations on the bacterial degradation of the alicyclic molecule cyclododecane. it represents a structure where the initial degradative steps have to be similar to a "subterminal" attack as there is no "terminal" part of the molecule. we were able to show that the gram-positive bacterium rhodococcus ruber cd4 dsm 44394 oxidizes cyclododecane to the corresponding alcohol and ketone, the latter being subject to ring fission by a baeyer-villiger oxygenase. this key e ... | 1999 | 10461374 |
| biosynthesis of polyhydroxyalkanoates from low-rank coal liquefaction products by pseudomonas oleovorans and rhodococcus ruber | a screening identified several bacteria that were able to use chemically heterogeneous low-rank coal liquefaction products as complex carbon sources for growth. pseudomonas oleovorans and rhodococcus ruber accumulated polyhydroxyalkanoic acids (pha) amounting to 2%-8% of the cell dry weight when the cells were cultivated on these liquefaction products in the absence of any other carbon source. r. ruber accumulated, in addition to pha, small amounts of triacylglycerols. the accumulated pha consis ... | 1999 | 10461375 |
| developments in destructive and non-destructive pathways for selective desulfurizations in oil-biorefining processes | biocatalytic desulfurization is still not a commercial technology, but conceptual engineering and sensitivity analyses have shown that the approach is very promising. the purpose of this paper is to investigate further some aspects of the biodesulphurization pathways, discussing the non-destructive pathway with the well-known rhodococcus rhodochrous igts8. findings revealed byproducts, such as 2'-hydroxybiphenyl (hbp), sulfite and sulfate, obtained by the desulfurization of dibenzothiophene (dbt ... | 1999 | 10461376 |
| batch culture biodegradation of methylhydrazine contaminated nasa wastewater. | the batch culture degradation of nasa wastewater containing mixtures of citric acid, methylhydrazine, and their reaction product was studied. the organic contaminants present in the nasa wastewater were degraded by achromobacter sp., rhodococcus b30 and rhodococcus j10. while the achromobacter sp. showed a preference for the degradation of the citric acid, the rhodococcus species were most effective in reducing the methylhydrazine and the reaction product. removals of more than 50% were observed ... | 1999 | 10466199 |
| steroid monooxygenase of rhodococcus rhodochrous: sequencing of the genomic dna, and hyperexpression, purification, and characterization of the recombinant enzyme. | steroid monooxygenase of rhodococcus rhodochrous is a baeyer-villigerase catalyzing the insertion of an oxygen atom between the c(17)- and c(20)-carbons of progesterone to produce testosterone acetate. the 5.1-kbp-long bamhi dna fragment containing the steroid monooxygenase gene, smo, was cloned from the chromosomal dna and sequenced. the smo gene is 1,650 nucleotides long, starts with a ttg codon, and ends with a tga codon. the deduced amino acid sequence indicates that the enzyme protein consi ... | 1999 | 10467180 |
| nitrile hydratase involved in aldoxime metabolism from rhodococcus sp. strain yh3-3 purification and characterization. | nitrile hydratase responsible for aldoxime metabolism from the e-pyridine-3-aldoxime degrading bacterium, rhodococcus sp. strain yh3-3 was purified and characterized. addition of cobalt ion was necessary for the formation of enzyme. the enzyme activity was highly induced not only by nitriles and amides but also by several aldoxime compounds. the enzyme was purified approximately 108-fold with a 16% yield from the cell-free extract of the strain. the native enzyme had a mr of approximately 130 00 ... | 1999 | 10469129 |
| rhodococcus equi infections: clinical features and laboratory diagnosis. | 1999 | 10472487 | |
| stereoselective carveol dehydrogenase from rhodococcus erythropolis dcl14. a novel nicotinoprotein belonging to the short chain dehydrogenase/reductase superfamily. | a novel nicotinoprotein, catalyzing the dichlorophenolindophenol-dependent oxidation of carveol to carvone, was purified to homogeneity from rhodococcus erythropolis dcl14. the enzyme is specifically induced after growth on limonene and carveol. dichlorophenolindophenol-dependent carveol dehydrogenase (cdh) is a homotetramer of 120 kda with each subunit containing a tightly bound nad(h) molecule. the enzyme is optimally active at ph 5.5 and 50 degrees c and displays a broad substrate specificity ... | 1999 | 10473585 |
| expression of a functional antibody fragment in the gut of rhodnius prolixus via transgenic bacterial symbiont rhodococcus rhodnii. | expression within insects of foreign antiparasitic gene products via microbial symbionts could be used to prevent transmission of vector-borne pathogens to vertebrate hosts. genetically transformed symbiotic bacteria rhodococcus rhodnii expressed functional antibody fragments (rdb3 encoding murine v(h)/k which binds progesterone) that were exported into the gut lumen of the triatomine bug rhodnius prolixus (hemiptera: reduviidae), a vector of chagas disease. transgenic symbionts were maintained ... | 1999 | 10484156 |
| [pulmonary malacoplakia caused by rhodococcus equi in aids: a case report]. | we describe the observation of a right upper lobe consolidation with cavitation produced by rhodococcus equi in a patient suffering from aids. the inefficacy of a prolonged antimicrobial therapy adapted against r. equi led to a right upper lobectomy. the histopathology showed a pseudotumoral mass, with dense infiltration of macrophages containing michaelis-gutmann bodies, which was positive for the culture of r. equi. pulmonary malacoplakia with rhodococcus equi was diagnosed. this pathology sho ... | 1999 | 10486839 |
| restriction fragment length polymorphisms of virulence plasmids in rhodococcus equi. | virulent rhodococcus equi, which is a well-known cause of pyogranulomatous pneumonia in foals, possesses a large plasmid encoding virulence-associated 15- to 17-kda antigens. foal and soil isolates from five countries-argentina, australia, canada, france, and japan-were investigated for the presence of 15- to 17-kda antigens by colony blotting, using the monoclonal antibody 10g5, and the gene coding for 15- to 17-kda antigens by pcr. plasmid dnas extracted from positive isolates were digested wi ... | 1999 | 10488224 |
| tnf receptor p55 is required for elimination of inflammatory cells following control of intracellular pathogens. | the elimination of lymphocytes within inflammatory lesions is a critical component in the resolution of disease once pathogens have been cleared. we report here that signaling through the tnf receptor p55 (tnfrp55) is required to eliminate lymphocytes from lesions associated with intracellular pathogens. thus, tnfrp55-/- mice, but not fas-deficient mice, maintained inflammatory lesions associated with either leishmania major or rhodococcus equi infection, although they developed a th1 response a ... | 1999 | 10490988 |
| purification and partial characterization of caffeine oxidase--a novel enzyme from a mixed culture consortium. | cell-free extract prepared from a mixed culture consisting of strains belonging to the genera klebsiella and rhodococcus grown in the presence of caffeine contains a novel enzyme, caffeine (1,3, 7-trimethylxanthine) oxidase which catalyzes the oxidation of caffeine at the c-8 position to produce 1,3,7-trimethyluric acid. the enzyme was purified to homogeneity by a combination of ion-exchange and hydrophobic column chromatographies. both native and sds/page of the purified enzyme showed a single ... | 1999 | 10491316 |
| nitrile hydratase from rhodococcus erythropolis: metabolization of steroidal compounds with a nitrile group. | the progestin dienogest (17alpha-cyanomethyl-17beta-hydroxy-estra-4,9-dien-3-one) was metabolized by the nitrile hydratase-containing microorganism rhodococcus erythropolis. an enzymatic hydrolysis of the nitrile group at the 17alpha-side chain was intended to obtain novel derivatives and to test them for progesterone receptor affinity. in contrast to the rapid enzymatic hydrolysis of nonsteroidal nitriles, the nitrile group of dienogest was cleaved very slowly. the dominant reaction was an arom ... | 1999 | 10493599 |
| modulation of cytokine response of pneumonic foals by virulent rhodococcus equi. | the ability of rhodococcus equi to induce pneumonia in foals depends on the presence of an 85- to 90-kb plasmid. in this study, we evaluated whether plasmid-encoded products mediate virulence by modulating the cytokine response of foals. foals infected intrabronchially with a virulence plasmid-containing strain of r. equi had similar gamma interferon (ifn-gamma) and interleukin-12 (il-12) p35 but significantly higher il-1beta, il-10, il-12 p40, and tumor necrosis factor alpha (tnf-alpha) mrna ex ... | 1999 | 10496876 |
| purification and characterization of a novel naphthalene dioxygenase from rhodococcus sp. strain ncimb12038. | we report here the characterization of the catalytic component (isp(nar)) of a new naphthalene dioxygenase from rhodococcus sp. strain ncimb12038. the genes encoding the two subunits of isp(nar) are not homologous to their previously characterized counterparts in pseudomonas. the deduced amino acid sequences have only 33 and 29% identity with the corresponding subunits in pseudomonas putida ncib 9816-4, for which the tertiary structure has been reported. | 1999 | 10498739 |
| essential tyrosine residues in 3-ketosteroid-delta(1)-dehydrogenase from rhodococcus rhodochrous. | tetranitromethane treatment of 3-ketosteroid-delta(1)-dehydrogenase of rhodococcus rhodochrous caused loss of the catalytic activity in a time- and concentration-dependent manner. peptides (p-81) and (pn-83) were isolated from tryptic digests of the native and tetranitromethane-treated enzyme proteins, respectively. pn-83 was the nitrated form of p-81. the amino acid sequence was ggaplidylesdddlefmvypwpdyfgk (positions 97-124 of the dehydrogenase sequence). pn-83 showed a low yield of pth-tyr of ... | 1999 | 10502672 |
| utilization of trihalogenated propanes by agrobacterium radiobacter ad1 through heterologous expression of the haloalkane dehalogenase from rhodococcus sp. strain m15-3. | trihalogenated propanes are toxic and recalcitrant organic compounds. attempts to obtain pure bacterial cultures able to use these compounds as sole carbon and energy sources were unsuccessful. both the haloalkane dehalogenase from xanthobacter autotrophicus gj10 (dhla) and that from rhodococcus sp. strain m15-3 (dhaa) were found to dehalogenate trihalopropanes to 2,3-dihalogenated propanols, but the kinetic properties of the latter enzyme are much better. broad-host-range dehalogenase expressio ... | 1999 | 10508091 |
| production and partial characterization of antibody to cord factor (trehalose 6,6'-dimycolate) in mice. | antibody production against the trehalose 6,6'-dimycolate (tdm, cord factor) of rhodococcus ruber, a non-pathogenic species of the actinomycetales group, was investigated in mice by repeated intraperitoneal injection of tdm in water-in-oil-in-water micelles without carrier protein. the antigenic tdm was isolated and purified chromatographically from the chloroform-methanol extractable lipids of r. ruber. the hydrophobic moiety of this tdm was composed of two molecules of monoenoic or dienoic alp ... | 1999 | 10524797 |
| bacteremia due to dietzia maris in an immunocompromised patient. | 1999 | 10524995 | |
| severe otitis and mastoiditis due to rhodococcus equi in a patient with aids. case report. | we report a case of otitis media associated with pneumonia due to rhodococcus equi. a 31-year-old patient with aids presented with cough and right facial palsy. imaging revealed right otitis media and severe temporal bone destruction, associated with pneumonia. r. equi was isolated from ear secretions, blood, and sputum. the radiologic findings are described. this unusual pathogen should be included in the differential diagnosis of the immunocompromised patient with aggressive otitis. | 1999 | 10525774 |
| cloning of an orf with homology to mycobacterium echa1, encoding the enoyl-coa hydratase, in rhodococcus fascians. | an open reading frame encoding a polypeptide of significant homology (55.7% identity) with the enoyl-coa hydratase encoded by the gene echa1 from mycobacterium tuberculosis has been found in the genome of the plant-pathogen bacteria rhodococcus fascians strain nrrl-b-15096. sequence alignments showed that it possesses several conserved blocks common to e. coli, m. tuberculosis and human mitochondria. one of such blocks includes a glutamate residue located at position 149, corresponding to the gl ... | 1999 | 10727085 |
| complement activation by mycoloyl glycolipids from mycobacterium tuberculosis and rhodococcus ruber. | in this study, we examined complement activation by mycoloyl glycolipids (mgl) such as trehalose-6,6'-dimycolate (tdm), often termed cord factor, and trehalose-6-monomycolate from mycobacterium tuberculosis and rhodococcus ruber, and also examined the effect of complement binding to mgl on phagocytosis by human monocytes. tdm and tmm, but not glucose mycolate, mannose mycolate or fructose mycolate which differ from tmm only in carbohydrate moiety, exhibited complement activation. tdm and tmm of ... | 1999 | 10730082 |
| characterization of is1676 from rhodococcus erythropolis sq1. | to develop a transposable element-based system for mutagenesis in rhodococcus, we used the sacb gene from bacillus subtilis to isolate a novel transposable element, is1676, from r. erythropolis sq1. this 1693 bp insertion sequence is bounded by imperfect (10 out of 13 bp) inverted repeats and it creates 4 bp direct repeats upon insertion. comparison of multiple insertion sites reveals a preference for the sequence 5'-(c/t)ta(a/g)-3' in the target site. is1676 contains a single, large (1446 bp) o ... | 1999 | 10616714 |
| an extractive membrane biofilm reactor for degradation of 1,3-dichloropropene in industrial waste water. | a bacterial biofilm, capable of mineralising a technical mixture of cis- and trans-1,3-dichloropropene (dcpe), was enriched on the biomedium side of an extractive membrane biofilm reactor (embr). the membrane separates the biomedium from the industrial waste water, in terms of ph, ionic strength and the concentration of toxic chemicals. the biofilm, attached to a silicone membrane, is able to mineralize dcpe after its diffusion through the membrane. five bacterial strains with degradation capabi ... | 1999 | 10616720 |
| analysis of the relative abundance of different types of bacteria capable of toluene degradation in a compost biofilter. | a microbial community of a compost biofilter treating toluene vapors was investigated using serum-bottle assays and mineral-agar plates. toluene was not consumed in the absence of oxygen. however, filter-bed extracts exposed to toluene vapor as the only carbon source produced distinct colony types (phenotypic groups) that were counted separately. strains from each group were isolated and checked for toluene-degradation activity in serum bottles. only 15% of colonies were true toluene degraders. ... | 1999 | 10616721 |
| a pentacoordinated di-n-carboxamido-dithiolato-o-sulfinato-iron(iii) complex related to the metal site of nitrile hydratase. | postcoordination oxidation by dioxygen of one of the thiolate groups in a pentadentate n(2)s(3) ligand results in an iron(iii) complex with two n-carboxamido, two thiolato, and one o-sulfinato ligands (see the cameron representation). this novel mixed coordination is similar to that determined for the inactive form of the nitrile hydratase from rhodococcus sp. n-771, but differs by the o versus s binding of the sulfinato ligand. | 1999 | 10602230 |
| effect of prophylactic administration of hyperimmune plasma to prevent rhodococcus equi infection on foals from endemically affected farms. | the effect on foals of prophylactic administration of hyperimmune plasma to prevent r. equi infection was investigated on three farms at which r. equi infection was endemic. sixteen foals between 10 and 39 days of age were intravenously given 1-21 of hyperimmune plasma. elisa antibody titres against r. equi were significantly increased and maintained at high levels for over 30 days in most of the recipient foals. the prevalence of r. equi infection was 6.3% (1/16) in the foals that received the ... | 1999 | 10605374 |
| search for an antibody profile of rhodococcus equi infection in aids patients despite the diversity of isolates and patient immune dysfunction. | diversity of virulence-associated antigens of rhodococcus equi was detected among thirteen strains isolated from aids patients on two continents. one out of four brazilian isolates presented the virulence-associated antigen of 15- to 17-kda, and the other three isolates had the 20-kda virulence-associated antigen. in contrast, only three out of nine italian isolates were positive for virulence-associated antigens - two for the 15- to 17-kda antigen and one for the 20-kda antigen. in four other i ... | 1999 | 10611743 |
| [pulmonary infection from rhodococcus equi after renal transplantation. review of the literature]. | rhodococcus equi, a strictly aerobic gram positive coco-bacillus, is a pathogen for horses and foals. it may induce opportunistic infections and is described in aids infected patients. we report the case of a 47-year old man, breeder of horses, with kidney transplant who has presented, 8 years after his graft, an impairment of health, a fever and evidence of pulmonary disease. the pulmonary biopsy under scanner guidance and microbiology study, has displayed the diagnosis of rhodococcus equi infe ... | 1999 | 10642987 |
| [bacteremic pneumonia due to rhodococcus equi in a patient with human immunodeficiency virus infection and visceral leishmaniasis]. | 1999 | 10650653 | |
| [equine rhodococcus equi pneumonia: first report in israel and its significance for man]. | rhodococcus equi is an important pathogen in young horses. in recent years it has been increasingly recognized as an opportunistic infectious agent in patients with immune deficiency. the increase in recognized cases may be related to the increased prevalence of aids. however, more cases may have been recognized lately due to increased awareness of the pathogenicity of this bacterium. based on medical reports, there appears to be an association between rhodococcus equi infections and exposure to ... | 1999 | 10959322 |
| degradation of anaerobic reductive dechlorination products of aroclor 1242 by four aerobic bacteria. | we studied the aerobic degradation of eight pcb congeners which comprise from 70 to 85% of the anaerobic dechlorination products from aroclor 1242, including 2-, 4-, 2,4-, 2,6-, 2,2'-, 2,4'-, 2,2', 4-, and 2,4,4'-chlorobiphenyl (cb), and the biodegradation of their mixtures designed to simulate anaerobic dechlorination profiles m and c. strains comamonas testosteroni vp44 and rhodococcus erythreus ny05 preferentially oxidized a para-substituted ring, while rhodococcus sp. rha1, similar to well k ... | 1999 | 10870552 |
| [conjugative transfer of a plasmid from escherichia coli to various strains of the order actinomycetales]. | the conjugal transfer of autonomous and integrative plasmids from the donor strain escherichia coli s17-1 to strains of genera actinomadura, arthrobacter, kitasatoa, micromonospora, nocardia, rhodococcus, saccharopolyspora, and to 16 strains of the genus streptomyces was demonstrated. the status of plasmids in recipient strains and the stability of their inheritance were analyzed. plasmids constructed for strains of the genus streptomyces were shown to function in a large number of strains belon ... | 1999 | 10687092 |
| antimicrobial activity of o-carboranylalanine. | functionalized polyhedral carboranes, including amino acid analogs, have unique physicochemical properties and are used as experimental anticancer agents. however, our current knowledge on their effect in nonmammalian biological systems is limited. we investigated the activity spectrum in vitro of o-carboranylalanine (o-cba), considered to be a highly lipophilic analog of phenylalanine, against representative plant pathogenic bacteria and fungi of various taxonomic position. the antibacterial ef ... | 1999 | 10707765 |
| search for virulence-associated antigens of rhodococcus equi in strains isolated from patients with acquired immunodeficiency syndrome. | rhodococcus equi (formerly corynebacterium equi) are known to be highly virulent, intermediate in virulence, or avirulent correlated with specific virulence-associated antigens identified immunochemically by different molecular weights. the association of virulence antigens with infection of aids patients by this organism has not been sufficiently evaluated in brazil or italy. the objective of the present study was to search for virulence-associated antigens of 15-to 17-kd and 20-kd in rhodococc ... | 1999 | 11084666 |
| microbial conversion of indene to indandiol: a key intermediate in the synthesis of crixivan. | indene is oxidized to mixtures of cis- and trans-indandiols and related metabolites by pseudomonas putida and rhodococcus sp. isolates. indene metabolism is consistent with monooxygenase and dioxygenase activity. p. putida resolves enantiomeric mixtures of cis-1,2-indandiol by further selective oxidation of the 1r, 2s-enantiomer yielding high enantiomeric purity of cis-(1s, 2r)-indandiol, a potential intermediate in the synthesis of indinavir sulfate (crixivan), a protease inhibitor used in the ... | 1999 | 10935755 |
| biodegradation of a high molecular weight aliphatic ether--indications of an unusual biodegradation pathway. | an aliphatic ether (1-phytanyl-1-octadecanyl-ether) of high molecular weight was used as a sole carbon source in degradation experiments with different aerobic bacteria. the enriched culture b5, obtained from fuel contaminated soils, was able to degrade the substance for more than 90%. a culture of rhodococcus ruber was similarly effective. detailed investigation of the metabolites allowed us to characterize an unusual degradation pathway via a mid-chain oxidation mechanism ('internal oxidative ... | 1999 | 11068823 |
| the bactericidal activities of hmr 3004, hmr 3647 and erythromycin against gram-positive bacilli and development of resistance. | the bactericidal activities of two new ketolides, hmr 3004 and hmr 3647, and the potential to develop resistance to these two antibiotics were studied in gram-positive bacilli. as judged by time-kill kinetics both ketolides were mostly bacteriostatic, being bactericidal against only highly susceptible isolates of corynebacterium striatum (two isolates) and corynebacterium minutissimum (one isolate). spontaneous resistant mutants were detected in seven of 30 strains tested, mainly in rhodococcus ... | 1999 | 11252337 |
| temperature effects and substrate interactions during the aerobic biotransformation of btex mixtures by toluene-enriched consortia and rhodococcus rhodochrous. | a microbial consortium derived from a gasoline-contaminated aquifer was enriched on toluene (t) in a chemostat at 20 degrees c and was found to degrade benzene (b), ethylbenzene (e), and xylenes (x). studies conducted to determine the optimal temperature for microbial activity revealed that cell growth and toluene degradation were maximized at 35 degrees c. a consortium enriched at 35 degrees c exhibited increased degradation rates of benzene, toluene, ethylbenzene, and xylenes in single-substra ... | 1999 | 10099561 |
| functional expression of nitrile hydratase in escherichia coli: requirement of a nitrile hydratase activator and post-translational modification of a ligand cysteine. | the nitrile hydratase (nhase) from rhodococcus sp. n-771 is a photoreactive enzyme that is inactivated on nitrosylation of the non-heme iron center and activated on photo-dissociation of nitric oxide (no). the nitrile hydratase operon consists of six genes encoding nhase regulator 2, nhase regulator 1, amidase, nhase alpha subunit, nhase beta subunit and nhase activator. we overproduced the nhase in escherichia coli using a t7 expression system. the nhase was functionally expressed in e. coli on ... | 1999 | 10101282 |
| development of an automated microbial sensor system. | an automated whole cell biosensor system was developed by integration of immobilized microbial cells in a flow-through system with screen-printed flow-through electrodes as detectors. the detectors used were thick-film pt-electrodes in a 3-electrode configuration constructed as sandwich flow-through cells with a volume of about 36 microliters polarized at -900 mv. the measuring principle was the determination of oxygen consumption due to the microbial metabolism. fructose was used as model analy ... | 1999 | 10101840 |
| cobalt proteins. | in the form of vitamin b12, cobalt plays a number of crucial roles in many biological functions. however, recent studies have provided information on the biochemistry and bioinorganic chemistry of several proteins containing cobalt in a form other than that in the corrin ring of vitamin b12. to date, eight noncorrin-cobalt-containing enzymes (methionine aminopeptidase, prolidase, nitrile hydratase, glucose isomerase, methylmalonyl-coa carboxytransferase, aldehyde decarbonylase, lysine-2,3-aminom ... | 1999 | 10103026 |
| the alkene monooxygenase from xanthobacter strain py2 is closely related to aromatic monooxygenases and catalyzes aromatic monohydroxylation of benzene, toluene, and phenol. | the genes encoding the six polypeptide components of the alkene monooxygenase from xanthobacter strain py2 (xamo) have been located on a 4.9-kb fragment of chromosomal dna previously cloned in cosmid pny2. sequencing and analysis of the predicted amino acid sequences indicate that the components of xamo are homologous to those of the aromatic monooxygenases, toluene 2-, 3-, and 4-monooxygenase and benzene monooxygenase, and that the gene order is identical. the genes and predicted polypeptides a ... | 1999 | 10103255 |
| effects of surfactant mixtures, including corexit 9527, on bacterial oxidation of acetate and alkanes in crude oil. | mixtures of nonionic and anionic surfactants, including corexit 9527, were tested to determine their effects on bacterial oxidation of acetate and alkanes in crude oil by cells pregrown on these substrates. corexit 9527 inhibited oxidation of the alkanes in crude oil by acinetobacter calcoaceticus atcc 31012, while span 80, a corexit 9527 constituent, markedly increased the oil oxidation rate. another corexit 9527 constituent, the negatively charged dioctyl sulfosuccinate (aot), strongly reduced ... | 1999 | 10103264 |
| influence of age and plasma treatment on neutrophil phagocytosis and cd18 expression in foals. | the aim of this study was to evaluate the influence of age and plasma treatment on neutrophil phagocytosis, cd18 expression and serum opsonic capacity in foals in field settings. microbial infections constitute a large threat in young foals and neutrophil functions are crucial for the defense. blood samples were obtained from 13 foals at seven time points between the ages of 2 and 56 days and once from 16 adult horses. six of the foals were treated with adult plasma at the age of 1 week. neutrop ... | 1999 | 10189199 |
| degradation of chloronitrobenzenes by a coculture of pseudomonas putida and a rhodococcus sp. | a single microorganism able to mineralize chloronitrobenzenes (cnbs) has not been reported, and degradation of cnbs by coculture of two microbial strains was attempted. pseudomonas putida hs12 was first isolated by analogue enrichment culture using nitrobenzene (nb) as the substrate, and this strain was observed to possess a partial reductive pathway for the degradation of nb. from high-performance liquid chromatography-mass spectrometry and 1h nuclear magnetic resonance analyses, nb-grown cells ... | 1999 | 10049867 |
| microbial desulfurization of organic sulfur compounds in petroleum. | sulfur removal from petroleum is important from the standpoint of the global environment because the combustion of sulfur compounds leads to the production of sulfur oxides, which are the source of acid rain. as the regulations for sulfur in fuels become more stringent, the existing chemical desulfurizations are coming inadequate for the "deeper desulfurization" to produce lower-sulfur fuels without new and innovative processes. biodesulfurization is rising as one of the candidates. several micr ... | 1999 | 10052116 |
| epoxide hydrolases and their synthetic applications. | chiral epoxides and 1,2-diols, which are central building blocks for the asymmetric synthesis of bioactive compounds, can be obtained by using enzymes--i.e. epoxide hydrolases--which catalyse the enantioselective hydrolysis of epoxides. these biocatalysis have recently been found to be more widely distributed in fungi and bacteria than previously expected. sufficient sources from bacteria, such as rhodococcus and nocardia spp., or fungi, as for instance aspergillus and beauveria spp., have now b ... | 1999 | 9933984 |
| hydride-meisenheimer complex formation and protonation as key reactions of 2,4,6-trinitrophenol biodegradation by rhodococcus erythropolis. | biodegradation of 2,4,6-trinitrophenol (picric acid) by rhodococcus erythropolis hlpm-1 proceeds via initial hydrogenation of the aromatic ring system. here we present evidence for the formation of a hydride-meisenheimer complex (anionic sigma-complex) of picric acid and its protonated form under physiological conditions. these complexes are key intermediates of denitration and productive microbial degradation of picric acid. for comparative spectroscopic identification of the hydride complex, i ... | 1999 | 9973345 |
| stereoselectivities of microbial epoxide hydrolases. | epoxide hydrolases from bacterial and fungal sources are highly versatile biocatalysts for the asymmetric hydrolysis of epoxides on a preparative scale. besides kinetic resolution, which yields the corresponding enantiomerically enriched vicinal diol and the remaining nonconverted epoxide, enantioconvergent processes are also possible, which lead to the formation of a single enantiomeric diol from a racemic oxirane. the data available to date indicate that the enantioselectivities of enzymes fro ... | 1999 | 10021403 |
| phenylalanine dehydrogenase from rhodococcus sp. m4: high-resolution x-ray analyses of inhibitory ternary complexes reveal key features in the oxidative deamination mechanism. | the molecular structures of recombinant l-phenylalanine dehydrogenase from rhodococcus sp. m4 in two different inhibitory ternary complexes have been determined by x-ray crystallographic analyses to high resolution. both structures show that l-phenylalanine dehydrogenase is a homodimeric enzyme with each monomer composed of distinct globular n- and c-terminal domains separated by a deep cleft containing the active site. the n-terminal domain binds the amino acid substrate and contributes to the ... | 1999 | 10029526 |
| isolation of a gene encoding a glycosylated cytokinin oxidase from maize. | the major cytokinin oxidase in immature maize kernels was purified to homogeneity. selected tryptic peptides were used to design degenerate oligonucleotide primers for pcr isolation of a fragment of the oxidase gene. hybridization of the pcr fragment to a maize genomic library allowed isolation of a full-length cytokinin oxidase gene (ckx1). the gene encodes a protein of approximately 57 kda that possesses a signal peptide, eight consensus n-glycosylation sequences and a consensus fad binding se ... | 1999 | 10049708 |
| analysis of the structural diversity of mycolic acids of rhodococcus and gordonia [correction of gordonla] isolates from activated sludge foams by selective ion monitoring gas chromatography-mass spectrometry (sim gc-ms) | a method using selective ion monitoring (sim) gas chromatography-mass spectrometry is described for analysis of mycolic acids which reveals a hitherto unrecognised chemical structural diversity among these in some members of the mycolata. the structural interpretation of mass spectral data of mycolic acids from rhodococcus spp and gordonia [corrected] spp using sim is discussed. | 1999 | 10076631 |
| hydrazide synthesis: novel substrate specificity of amidase. | the amidase from rhodococcus rhodochrous j1, which hydrolyses amide to acid and ammonia, was found to catalyze the synthesis of hydrazide using hydrazine as a substrate. this is the first report on the hydrazide synthesis through enzymatic reactions. the enzyme also acted on benzoic acid in the presence of hydrazine, yielding benzoic hydrazide. together with the finding that benzoic hydrazide was converted into benzoic acid (when it was used as a substrate in the absence of hydrazine), these uni ... | 1999 | 10079199 |
| pulmonary malacoplakia associated with rhodococcus equi infection in a patient with aids. | an aids patient with a cavitary lung lesion was found to have pulmonary malacoplakia associated with rhodococcus equi infection. the diagnosis was based on the typical histologic features of transbronchial biopsy and a positive bacterial culture. all 13 reported cases of aids patients with pulmonary malacoplakia were associated with r equi. the recognition of this unique entity is important because of its responsiveness to therapy. | 1999 | 10084511 |
| degradation of 1,2-dibromoethane by mycobacterium sp. strain gp1. | the newly isolated bacterial strain gp1 can utilize 1, 2-dibromoethane as the sole carbon and energy source. on the basis of 16s rrna gene sequence analysis, the organism was identified as a member of the subgroup which contains the fast-growing mycobacteria. the first step in 1,2-dibromoethane metabolism is catalyzed by a hydrolytic haloalkane dehalogenase. the resulting 2-bromoethanol is rapidly converted to ethylene oxide by a haloalcohol dehalogenase, in this way preventing the accumulation ... | 1999 | 10094681 |
| purification of a glutathione s-transferase and a glutathione conjugate-specific dehydrogenase involved in isoprene metabolism in rhodococcus sp. strain ad45. | a glutathione s-transferase (gst) with activity toward 1, 2-epoxy-2-methyl-3-butene (isoprene monoxide) and cis-1, 2-dichloroepoxyethane was purified from the isoprene-utilizing bacterium rhodococcus sp. strain ad45. the homodimeric enzyme (two subunits of 27 kda each) catalyzed the glutathione (gsh)-dependent ring opening of various epoxides. at 5 mm gsh, the enzyme followed michaelis-menten kinetics for isoprene monoxide and cis-1, 2-dichloroepoxyethane, with vmax values of 66 and 2.4 micromol ... | 1999 | 10094686 |
| heterologous expression of alkene monooxygenase from rhodococcus rhodochrous b-276. | alkene monooxygenase (amo) from rhodococcus rhodochrous (formerly nocardia corallina) b-276 is a three-component enzyme system encoded by the four-gene operon amoabcd. amo catalyses the stereoselective epoxygenation of aliphatic alkenes, yielding primarily r enantiomers. the presumed site of alkene oxygenation is a dinuclear iron centre similar to that in the soluble methane monooxygenases of methanotrophic bacteria, to which amo exhibits a significant degree of amino acid sequence identity. the ... | 1999 | 10095780 |
| effect of aromatic compounds on cellular fatty acid composition of rhodococcus opacus. | in cells of rhodococcus opacus gm-14, gm-29, and 1cp, the contents of branched (10-methyl) fatty acids increased from 3% to 15 to 34% of the total fatty acids when the cells were grown on benzene, phenol, 4-chlorophenol, chlorobenzene, or toluene as the sole source of carbon and energy, in comparison with cells grown on fructose. in addition, the content of trans-hexadecenoic acid increased from 5% to 8 to 18% with phenol or chlorophenol as the carbon source. the 10-methyl branched fatty acid co ... | 1999 | 9925629 |
| 3-nitroadipate, a metabolic intermediate for mineralization of 2, 4-dinitrophenol by a new strain of a rhodococcus species. | the bacterial strain rb1 has been isolated by enrichment cultivation with 2,4-dinitrophenol as the sole nitrogen, carbon, and energy source and characterized, on the basis of 16s rrna gene sequence comparison, as a rhodococcus species closely related to rhodococcus opacus. rhodococcus sp. strain rb1 degrades 2,4-dinitrophenol, releasing the two nitro groups from the compound as nitrite. the release of nitro groups from 2,4-dinitrophenol occurs in two steps. first, the 2-nitro group is removed as ... | 1999 | 9864324 |
| a sensitive, viable-colony staining method using nile red for direct screening of bacteria that accumulate polyhydroxyalkanoic acids and other lipid storage compounds. | the oxazine dye nile blue a and its fluorescent oxazone form, nile red, were used to develop a simple and highly sensitive staining method to detect poly(3-hydroxybutyric acid) and other polyhydroxyalkanoic acids (phas) directly in growing bacterial colonies. in contrast to previously described methods, these dyes were directly included in the medium at concentrations of only 0.5 microgram/ml, and growth of the cells occurred in the presence of the dyes. this allowed an estimation of the presenc ... | 1999 | 9914303 |
| microbial desulfurization of a crude oil middle-distillate fraction: analysis of the extent of sulfur removal and the effect of removal on remaining sulfur. | rhodococcus sp. strain ecrd-1 was evaluated for its ability to desulfurize a 232 to 343 degrees c middle-distillate (diesel range) fraction of oregon basin (ob) crude oil. ob oil was provided as the sole source of sulfur in batch cultures, and the extent of desulfurization and the chemical fate of the residual sulfur in the oil after treatment were determined. gas chromatography (gc), flame ionization detection, and gc sulfur chemiluminesce detection analysis were used to qualitatively evaluate ... | 1999 | 9872778 |
| rapid identification of clinically relevant nocardia species to genus level by 16s rrna gene pcr. | two regions of the gene coding for 16s rrna in nocardia species were selected as genus-specific primer sequences for a pcr assay. the pcr protocol was tested with 60 strains of clinically relevant nocardia isolates and type strains. it gave positive results for all strains tested. conversely, the pcr assay was negative for all tested species belonging to the most closely related genera, including dietzia, gordona, mycobacterium, rhodococcus, streptomyces, and tsukamurella. besides, unlike the la ... | 1999 | 9854071 |
| a novel gene-samfr involved in early stage of streptomyces ansochromogenes differentiation. | a 4.6 kb dna fragment was cloned from the dna library of streptomyces ansochromogenes using a partial dna fragment located in the downstream of promoter-p(th4) as probe. the experiments revealed that this dna fragment consists of saw d gene and a 1.4 kb pvu ii fragment which can accelerate mycelium formation o fs. ansochromogenes. the nucleotide sequence of 1.4 kb dna fragment was determined and analysed; the result indicated that the fragment contains one complete open reading frame (orf) which ... | 1999 | 18726478 |
| purification and characterization of dibenzothiophene (dbt) sulfone monooxygenase, an enzyme involved in dbt desulfurization, from rhodococcus erythropolis d-1. | dibenzothiophene (dbt), a model of organic sulfur compound in petroleum, is microbially desulfurized to 2-hydroxybiphenyl by rhodococcus erythropolis d-1. three desulfurization (dsz) enzymes--dszc, a, and b--and flavin reductase are involved in sulfur-specific dbt desulfurization. in this study, dsza was purified, characterized, and crystallized from r. erythropolis d-1. dsza, dbt sulfone monooxygenase, is the second enzyme in microbial dbt desulfurization metabolism and catalyzes the conversion ... | 1999 | 16232672 |