Publications

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[effect of cultivation parameters of antarctic strains enterobacter hormaechei and brevibacterium antarcticumon resistant to copper(ii) ions].enterobacter hormaechei and brevibacterium antarcticum strains isolated from ornithogenic soils of galindez island (west antarctica) were investigated for their resistance to cu2+ cations and for their capacity to cu2+ uptake from the environment. the studied strains are capable to grow in the concentration range of copper 100-1100 mg/l and to extract 11-75% of cu2+ from the environment depending on cultivation parameters and copper output concentration in the culture medium.200919938609
a polyphasic study on the species diversity of the sediment microbiota of lake hévíz.lake hévíz is the largest natural thermal lake of europe, harboring special bacterial communities. the aim of the present study was to gain information about the distribution and species diversity of the sediment microbiota, with special focus on actinobacteria, by using cultivation-based and -independent molecular methods. samples from two depths were taken in two different locations in october 2007. 245 strains were isolated, grouped to 85 otus by ardra, and identified by 16s rdna sequencing. ...200920038486
[biosynthesis of l-lysine-alpha-oxidase, an antitumor enzyme, by trichoderma spp].strains of trichoderma spp., producing l-lysine-alpha-oxidase, prospective in chemotherapy of malignant tumors, were studied. the best results of the enzyme biosynthesis were observed on the wheat bran medium. when grown on the media with various ph levels, the strains showed different spectra of the l-aminooxidase activity. the highest activity of the strains was recorded with respect to destruction of l-lysine. the lysine-producing organism brevibacterium sp. induced l-lysine-alpha-oxidase act ...200920052910
microbial ecology of gorgonzola rinds and occurrence of different biotypes of listeria monocytogenes.in this study we investigated the microbiota of gorgonzola rinds and maturing shelf swabs collected in 5 different maturing cellars in the northwest part of italy, in association with the detection and characterization of listeria monocytogenes. culture-dependent and -independent methods were performed in order to profile the main microbial populations present on the rinds and in the maturing shelves and species-specific pcr and pulsed field gel electrophoresis (pfge) were used to identify and t ...200919477544
identification of a brevibacterium marker gene specific to poultry litter and development of a quantitative pcr assay.to identify a dna sequence specific to a bacterium found in poultry litter that was indicative of faecal contamination by poultry sources.201020092540
extracellular production of riboflavin-binding protein, a potential bitter inhibitor, by brevibacillus choshinensis.riboflavin-binding protein (rbp) is a glycophosphoprotein found in hen eggs. we previously identified the extraordinary characteristic of rbp in reducing bitterness. for a more detailed study on the mode of action and industrial application of this characteristic, we investigated the microbial production of recombinant rbp (rrbp). we constructed a chicken rbp gene expression vector by inserting the rbp cdna in pncmo2, the escherichia coli-brevibacillus choshinensis shuttle vector. b. choshinensi ...201020045733
structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases.2,3-butanediol dehydrogenase (bdh) catalyzes the nad-dependent redox reaction between acetoin and 2,3-butanediol. there are three types of homologous bdh, each stereospecific for both substrate and product. to establish how these homologous enzymes possess differential stereospecificities, we determined the crystal structure of l-bdh with a bound inhibitor at 2.0 a. comparison with the inhibitor binding mode of meso-bdh highlights the role of a hydrogen-bond from a conserved trp residue(192). si ...201019941855
optimization and characterization of a new lipopeptide biosurfactant produced by marine brevibacterium aureum msa13 in solid state culture.the biosurfactant production of a marine actinobacterium brevibacterium aureum msa13 was optimized using industrial and agro-industrial solid waste residues as substrates in solid state culture (ssc). based on the optimization experiments, the biosurfactant production by msa13 was increased to threefold over the original isolate under ssc conditions with pre-treated molasses as substrate and olive oil, acrylamide, fecl(3) and inoculums size as critical control factors. the strain b. aureum msa13 ...201019959354
diversity and evolution of the phenazine biosynthesis pathway.phenazines are versatile secondary metabolites of bacterial origin that function in biological control of plant pathogens and contribute to the ecological fitness and pathogenicity of the producing strains. in this study, we employed a collection of 94 strains having various geographic, environmental, and clinical origins to study the distribution and evolution of phenazine genes in members of the genera pseudomonas, burkholderia, pectobacterium, brevibacterium, and streptomyces. our results con ...201020008172
molecular identification of airborne bacteria associated with aerial spraying of bovine slurry waste employing 16s rrna gene pcr and gene sequencing techniques.polymerase chain reaction amplification of the universal 16s ribosomal rna (rrna) gene was performed on a collection of 38 bacterial isolates, originating from air sampled immediately adjacent to the agricultural spreading of bovine slurry. a total of 16 bacterial genera were identified including both gram-positive and gram-negative genera. gram-positive organisms accounted for 34/38 (89.5%) of total bacterial numbers consisting of 12 genera and included staphylococcus (most common genus isolate ...201020018377
3,3'-dihydroxyisorenieratene prevents uv-induced formation of reactive oxygen species and the release of protein-bound zinc ions in human skin fibroblasts.3,3'-dihydroxyisorenieratene (dhir) is a structurally unusual carotenoid exhibiting bifunctional antioxidant properties. it is synthesized by brevibacterium linens, used in dairy industry for the production of red smear cheeses. the compound protects cellular structures against photo-oxidative damage and inhibits the uv-dependent formation of thymidine dimers. here we show that dhir prevents a uv-induced intracellular release of zinc ions from proteins in human dermal fibroblasts. the effect is ...201019862772
construction of a novel shuttle vector for use in brevibacterium flavum, an industrial amino acid producer.brevibacterium flavum has been developed to produce amino acids l-valine, l-lysine and l-threonine. however, there are not enough vectors available for the research on metabolic engineering in brevibacterium flavum. here we have constructed a shuttle expression vector pdxw-8 between escherichia coli and b. flavum. the vector harbors an origin orie for replicating in e. coli, a second origin oric for replicating in b. flavum, a large multiple cloning site including 11 single restriction enzyme si ...201019913575
production of isotopically labeled heterologous proteins in non-e. coli prokaryotic and eukaryotic cells.the preparation of stable isotope-labeled proteins is necessary for the application of a wide variety of nmr methods, to study the structures and dynamics of proteins and protein complexes. the e. coli expression system is generally used for the production of isotope-labeled proteins, because of the advantages of ease of handling, rapid growth, high-level protein production, and low cost for isotope-labeling. however, many eukaryotic proteins are not functionally expressed in e. coli, due to pro ...201019787297
brevibacterium pityocampae sp. nov., isolated from caterpillars of thaumetopoea pityocampa (lepidoptera, thaumetopoeidae).this work deals with the taxonomic study of a bacterium, strain tp12(t), isolated from caterpillars of the pine processionary moth (thaumetopoea pityocampa denis & schiffermüller, 1775; lepidoptera, thaumetopoeidae). the isolate was assigned to the genus brevibacterium on the basis of a polyphasic taxonomic study, including morphological and biochemical characteristics, 16s rrna gene sequence analysis, fatty acid analysis and dna g+c content. the highest 16s rrna gene sequence similarity to this ...201019651741
assignment of brevibacterium stationis (zobell and upham 1944) breed 1953 to the genus corynebacterium, as corynebacterium stationis comb. nov., and emended description of the genus corynebacterium to include isolates that can alkalinize citrate.brevibacterium stationis atcc 14403(t), corynebacterium ammoniagenes atcc 6872 and two clinical isolates were found to form a single taxon group consistent with the genus corynebacterium, designated here as corynebacterium stationis comb. nov. the type strain of corynebacterium stationis is atcc 14403(t) =ccug 43497( t) =cip 104228(t) =dsm 20302(t) =nbrc 12144(t) =jcm 11611(t) =vkm b-1228(t). these strains can utilize citrate; therefore, inclusion of c. stationis requires that the description of ...201019661509
brevibacterium sandarakinum sp. nov., isolated from a wall of an indoor environment.a gram-stain-positive, rod-shaped, non-endospore-forming, orange-pigmented (coloured) actinobacterium (01-je-003(t)) was isolated from the wall of an indoor environment primarily colonized with moulds. on the basis of 16s rrna gene sequence similarity studies, strain 01-je-003(t) was shown to belong to the genus brevibacterium and was most similar to the type strains of brevibacterium picturae (98.8 % similarity), brevibacterium marinum (97.3 %) and brevibacterium aurantiacum (97.2 %). chemotaxo ...201019661519
brevibacterium salitolerans sp. nov., an actinobacterium isolated from salt-lake sediment.a novel bacterium, designated trm 415(t), belonging to the genus brevibacterium, was isolated from a sediment sample from a salt lake in xinjiang province, china. comparative 16s rrna gene sequence analysis indicated that strain trm 415(t) was phylogenetically most closely related to brevibacterium album yim 90718(t) (98.4 % sequence similarity) and had low similarity (<95.5 %) to other species of the genus brevibacterium; however, dna-dna hybridization studies between strain trm 415(t) and b. a ...201020118289
surface microbiota analysis of taleggio, gorgonzola, casera, scimudin and formaggio di fossa italian cheeses.the composition of the bacterial consortia of the smear italian cheeses and their role on quality and safety is still poorly understood. the objective of this study was to identify and characterize the bacterial communities present on the surface of five traditional italian cheeses, casera valtellina, scimudin, formaggio di fossa, gorgonzola and taleggio. dgge analysis performed using total dna obtained from cheese surfaces enabled us to identify the dominant bacterial populations. bands showing ...201020167385
biosynthesis of silver and gold nanoparticles using brevibacterium casei.the present study demonstrates an unprecedented green process for the production of spherical-shaped au and ag nanoparticles synthesized and stabilized using a bacterium, brevibacterium casei. aqueous solutions of chloroaurate ions for au and ag(+) ions for silver were treated with b. casei biomass for the formation of au nanoparticles (aunp) and ag nanoparticles (agnp). the nanometallic dispersions were characterized by surface plasmon absorbance measuring at 420 and 540 nm for ag and au nanopa ...201020197229
brevibacillus expression system: host-vector system for efficient production of secretory proteins.brevibacillus expression system is an effective bacterial expression system for secretory proteins. the host bacterium, brevibacillus choshinensis, a gram-positive bacterium, has strong capacity to secrete a large amount of proteins (approximately 30 g/l), which mostly consist of cell wall protein. a host-vector system that utilizes such high expression capacity has been constructed for the production of secretory proteins and tested for various heterologous proteins, including cytokines, enzyme ...201020210747
population dynamics of two antilisterial cheese surface consortia revealed by temporal temperature gradient gel electrophoresis.surface contamination of smear cheese by listeria spp. is of major concern for the industry. complex smear ecosystems have been shown to harbor antilisterial potential but the microorganisms and mechanisms involved in the inhibition mostly remain unclear, and are likely related to complex interactions than to production of single antimicrobial compounds. bacterial biodiversity and population dynamics of complex smear ecosystems exhibiting antilisterial properties in situ were investigated by tem ...201020222967
vinyl acetate degradation by brevibacillus agri isolated from a slightly aerated methanogenic reactor.in a previous paper, the authors showed that a slight aeration of a methanogenic reactor treating wastewater from the manufacture of polymeric resins could improve its performance, by increasing or allowing the removal of some of its contaminants, including vinyl acetate (va). this paper reports the isolation under aerobic conditions of a va-biodegrading axenic culture (strain c1) retrieved from the sludge of a slightly aerated methanogenic reactor at 1 mg l(-1) d(-1) of dissolved oxygen (do). t ...201020232673
characterization of heterotrophic bacteria isolated from the biofilm of a kitchen sink.heterotrophic bacteria constituting the biofilm produced in a kitchen sink drain were analyzed, and the biofilm formation abilities and the hydrophobicity of the cell surface layer were measured for the isolates. when the biofilm sample was cultured at 36 degrees c and 25 degrees c for 7 days, there were about 10 times more colonies on oligotrophic r2a agar medium than on eutrophic bhi agar medium. from isolates from the biofilm sample, 13 bacterial species were detected. to examine the biofilm ...201020361519
bacterial diversity in amblyomma americanum (acari: ixodidae) with a focus on members of the genus rickettsia.the lone star tick, amblyomma americanum (acari: ixodidae), is commonly reported from people and animals throughout the eastern u.s. and is associated with transmission of a number of emerging diseases. to better define the microbial communities within lone star ticks, 16s rrna gene based analysis using bacteria-wide primers, followed by sequencing of individual clones (n = 449) was used to identify the most common bacterial operational taxonomic units (otus) present within colony-reared and wil ...201020380308
culture-dependent and culture-independent diversity of actinobacteria associated with the marine sponge hymeniacidon perleve from the south china sea.in this report, the diversity of actinobacteria associated with the marine sponge hymeniacidon perleve collected from a remote island of the south china sea was investigated employing classical cultivation and characterization, 16s rdna library construction, 16s rdna-restriction fragment length polymorphism (rdna-rflp) and phylogenetic analysis. a total of 184 strains were isolated using seven different media and 24 isolates were selected according to their morphological characteristics for phyl ...201020383659
comparative study of structurally related peptidoglycan monomer and muramyl dipeptide on humoral igg immune response to ovalbumin in mouse.structurally related peptidoglycan monomer (pgm) and muramyl dipeptide (mdp) differ in several aspects of biological activity but have in common immunostimulating properties. comparative study of the effects of these adjuvants on humoral igg immune response specific for protein antigen ovalbumin (ova) was carried out in two inbred mouse strains, cba and nih/olahsd, and their ability to modulate the bias of immune response towards th1/th2 was evaluated. mdp had better adjuvant activity at some po ...201020403461
production of recombinant cholesterol oxidase containing covalently bound fad in escherichia coli.cholesterol oxidase is an alcohol dehydrogenase/oxidase flavoprotein that catalyzes the dehydrogenation of c(3)-oh of cholesterol. it has two major biotechnological applications, i.e. in the determination of serum (and food) cholesterol levels and as biocatalyst providing valuable intermediates for industrial steroid drug production. cholesterol oxidases of type i are those containing the fad cofactor tightly but not covalently bound to the protein moiety, whereas type ii members contain covalen ...201020409334
[microbiological analysis of terrestrial biotopes of the antarctic region].microbiological analysis has been made of 120 samples from biotopes of the western coast of the antarctic peninsula (rasmussen cope, tuxen cope, waugh mountain), argentine archipelago islands (galindez, skua, corner, barchans, irizar, uruguay, cluls, three little pigs, king-george), as well as neighbouring islands (petermann--on the north, a group of jalour islands--on the east, berthelot--on the south-east); and more remote islands (darboux, lippmann, booth). it was found out that the total num ...201020455435
the type of cheese curds determined the colouring capacity of brevibacterium and arthrobacter species.this study compares the colouring capacity of brevibacterium aurantiacum (ba), brevibacterium bl and arthrobacter species as in relation to deacidified media made from lactic curd (epoisses), mixed curds (munster) and rennet curds (livarot or reblochon). ba colouring capacity proved to be constant, leading to a dark orange colour, irrespective of the deacidified media. however, it gave too dark a colour for reblochon. the strains bl and as were not adapted to the colouring of epoisses deacidifie ...201020462468
novel linear megaplasmid from brevibacterium sp. isolated from extreme environment.brevibacterium sp. ap13, isolated from flamingo's feces in laguna aparejos, a high-altitude lake located at approximately 4,200 m in the northwest of argentina was previously found to be resistant to multiple antibiotics, and was therefore screened for plasmids that may be implicated in antibiotic resistance. brevibacterium sp. ap13 was found to contain two plasmids of approximately 87 and 436 kb, designated pap13 and pap13c, respectively. only pap13 was stably maintained and was extensively cha ...201020473959
redesign, reconstruction, and directed extension of the brevibacterium linens c40 carotenoid pathway in escherichia coli.in this study, the carotenoid biosynthetic pathways of brevibacterium linens dsmz 20426 were reconstructed, redesigned, and extended with additional carotenoid-modifying enzymes of other sources in a heterologous host escherichia coli. the modular lycopene pathway synthesized an unexpected carotenoid structure, 3,4-didehydrolycopene, as well as lycopene. extension of the novel 3,4-didehydrolycopene pathway with the mutant pantoea lycopene cyclase crty(2) and the rhodobacter spheroidene monooxyge ...201020525861
biofilm disruption potential of a glycolipid biosurfactant from marine brevibacterium casei.the antibiofilm activity of a glycolipid biosurfactant isolated from the marine actinobacterium brevibacterium casei msa19 was evaluated against pathogenic biofilms in vitro. the isolate b. casei msa19 was a potential biosurfactant producer among the 57 stable strains isolated from the marine sponge dendrilla nigra. the biosurfactant production was optimized under submerged fermentation. the purified glycolipid showed a broad spectrum of antimicrobial activity. based on the minimum inhibitory co ...201020528933
synthesis of silver nanoparticles by glycolipid biosurfactant produced from marine brevibacterium casei msa19.the surfactants are emerging as potential nanoparticle stabilizing agents, however, the synthetic surfactants are not economically viable as well as they are not environmentally friendly. therefore, the biosurfactants are emerging as a green alternate for the synthesis and stabilization of nanoparticles. in this report, a glycolipid biosurfactant was produced from sponge-associated marine brevibacterium casei msa19 under solid state fermentation using the agro-industrial and industrial waste as ...201020600381
simultaneous chromate reduction and azo dye decolourization by brevibacterium casei: azo dye as electron donor for chromate reduction.chromate [cr(vi)] and azo dyes are common pollutants which may co-exist in some industrial effluents. hence studies of biological treatment of industrial wastewater should include investigation of the co-removal of these two pollutants. brevibacterium casei, which can reduce cr(vi) in the presence of the azo dye acid orange 7 (ao7) under nutrient-limiting condition, was isolated from a sewage sludge sample of a dyeing factory. response surface methodology, which is commonly used to optimize grow ...201020656406
[wound infections due to opportunistic corynebacterium species].wound infections are often due to endogenous bacterial flora which penetrates into a site of injury. the establishment of the etiologic agent can be problematic, especially when opportunistic bacteria are present, suggesting contamination of clinical material. among bacteria that can cause such diagnostic problems are opportunistic corynebacterium spp. and coryneforms colonizing skin. the aim of the study was to analyze the 24 clinical samples collected from wounds of different location, with gr ...201020873486
ftir-based polyphasic identification of lactic acid bacteria isolated from traditional greek graviera cheese.this study used a combination of phenotypic, physical (fourier transformed infra-red [ftir] spectroscopy) and molecular (rflp and sscp analysis of 16s rrna genes) methods to identify the lactic acid bacteria (lab) flora present in traditional greek graviera cheese after five weeks of ripening. a total of 300 isolates collected from high dilution plates of tsaye (incubated at 30 °c), m-17 (22 °c) and m-17 (42 °c) agar media were clustered by ftir and then representative strains of each cluster we ...201021056778
diversity and phylogeny of plant growth-promoting bacilli from moderately acidic soil.the molecular diversity of aerobic endospore-forming bacteria, typically bacillus and its derived genera, has been investigated in various environments. however, there have been few investigations concerning bacillus in acidic soils. in this study, the genotypic diversity and phylogenetic relationships among plant growth-promoting (pgp) bacilli isolated from the rice rhizosphere growing in acidic soils of kerala (ph varying from 6.3 to 6.8) were investigated. for assessing their biocontrol poten ...201021077114
isolation, characterization, and use for plant growth promotion under salt stress, of acc deaminase-producing halotolerant bacteria derived from coastal soil.in total, 140 halotolerant bacterial strains were isolated from both the soil of barren fields and the rhizosphere of six naturally growing halophytic plants in the vicinity of the yellow sea, near the city of incheon in the republic of korea. all of these strains were characterized for multiple plant growth promoting traits, such as the production of indole acetic acid (iaa), nitrogen fixation, phosphorus (p) and zinc (zn) solubilization, thiosulfate (s2o3) oxidation, the production of ammonia ...201021124065
purification and gene cloning of an enantioselective thioesterification enzyme from brevibacterium ketoglutamicum ku1073, a deracemization bacterium of 2-(4-chlorophenoxy)propanoic acid.we succeeded in the purification and gene cloning of a new enzyme, α-methyl carboxylic acid deracemizing enzyme 1 (mcad1) from brevibacterium ketoglutamicum ku1073, which catalyzes the (s)-enantioselective thioesterification reaction of 2-(4-chlorophenoxy)propanoic acid (cppa). the cloned gene of mcad1 contained an orf of 1,623 bp, encoding a polypeptide of 540 amino acids. in combination with cofactors atp, coenzyme a (coash), and mg(2+), mcad1 demonstrated perfect enantioselectivity toward cpp ...201021150124
a hyperactive cobalt-substituted extradiol-cleaving catechol dioxygenase.homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum (hpcd) has an fe(ii) center in its active site that can be replaced with mn(ii) or co(ii). whereas mn-hpcd exhibits steady-state kinetic parameters comparable to those of fe-hpcd, co-hpcd behaves somewhat differently, exhibiting significantly higher [formula: see text] and k (cat). the high activity of co-hpcd is surprising, given that cobalt has the highest standard m(iii/ii) redox potential of the three metals. comparison of the x- ...201021153851
global regulation of the response to sulfur availability in the cheese-related bacterium brevibacterium aurantiacum.in this study, we combined metabolic reconstruction, growth assays, and metabolome and transcriptome analyses to obtain a global view of the sulfur metabolic network and of the response to sulfur availability in brevibacterium aurantiacum. in agreement with the growth of b. aurantiacum in the presence of sulfate and cystine, the metabolic reconstruction showed the presence of a sulfate assimilation pathway, thiolation pathways that produce cysteine (cyse and cysk) or homocysteine (metx and mety) ...201021169450
[diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from xiaoxi national natural reserve, hunan province].to investigate the diversity of cultivable halophilic and halotolerant bacteria isolated from ordinary non-saline soil samples collected from xiaoxi national natural reserve (28 degrees 42' 15"-28 degrees 53' 15" n, 110 degrees 6' 50"-110 degrees 21' 35" e), hunan province, china.201021268889
combined dissolved oxygen and ph control strategy to improve the fermentative production of l-isoleucine by brevibacterium lactofermentum.the effect of both dissolved oxygen (do) and ph on l: -isoleucine production by batch culture of brevibacterium lactofermentum was investigated. a two-stage agitation speed control strategy was developed, and the isoleucine production reached 23.3 g l(-1) in a relative short time (52 h), increased by 11.6% compared to the results obtained in the single agitation speed control process. in order to make sure whether the combination of do and ph control can boost the production by a mutual effect, ...201019449037
correlation of quantitative pcr for a poultry-specific brevibacterium marker gene with bacterial and chemical indicators of water pollution in a watershed impacted by land application of poultry litter.the impact of fecal contamination from human and agricultural animal waste on water quality is a major public health concern. identification of the dominant source(s) of fecal pollution in a watershed is necessary for assessing the safety of recreational water and protecting water resources. a field study was conducted using quantitative pcr (qpcr) for the 16s rrna gene of brevibacterium sp. la35 to track feces-contaminated poultry litter in environmental samples. based on sensitivity and specif ...201121278274
in vivo self-hydroxylation of an iron-substituted manganese-dependent extradiol cleaving catechol dioxygenase.the homoprotocatechuate 2,3-dioxygenase from arthrobacter globiformis (mndd) catalyzes the oxidative ring cleavage reaction of its catechol substrate in an extradiol fashion. although this reactivity is more typically associated with non-heme iron enzymes, mndd exhibits an unusual specificity for manganese(ii). mndd is structurally very similar to the iron(ii)-dependent homoprotocatechuate 2,3-dioxygenase from brevibacterium fuscum (hpcd), and we have previously shown that both mndd and hpcd are ...201121279661
enhancement of growth and salt tolerance of red pepper seedlings (capsicum annuum l.) by regulating stress ethylene synthesis with halotolerant bacteria containing 1-aminocyclopropane-1-carboxylic acid deaminase activity.three 1-aminocyclopropane-1-carboxylic acid (acc) deaminase-producing halotolerant bacteria were isolated from west coast soil of yellow sea, incheon, south korea and evaluated for their efficiency in improving red pepper plant growth under salt stress. the strains rs16, rs656 and rs111 were identified by 16s rrna gene sequencing as brevibacterium iodinum, bacillus licheniformis and zhihengliuela alba, respectively. two hour exposure of 100, 150 and 200 mm nacl stress on 8 day old red pepper see ...201121300550
facultative methylotrophs from the human oral cavity and methylotrophy in strains of gordonia, leifsonia, and microbacterium.we show that bacteria with methylotrophic potential are ubiquitous in the human mouth microbiota. numerous strains of actinobacteria (brevibacterium, gordonia, leifsonia, microbacterium, micrococcus, rhodococcus) and proteobacteria (achromobacter, klebsiella, methylobacterium, pseudomonas, ralstonia) were isolated, and one strain of each of the eleven genera was studied in detail. these strains expressed enzymes associated with methylotrophic metabolism (methanol, methylamine, and formate dehydr ...201121374057
affinity purification of a cholesterol oxidase expressed in escherichia coli.a cholesterol oxidase (cod) gene from brevibacterium sp. (dq345780) was expressed in escherichia coli bl21 (de3), an affinity protocol was developed for the preparation, and industrial application of this method was of great potential. riboflavin was chosen as the affinity ligand, and it was coupled with sepharose 4b through some spacers. with the affinity medium, the purification process consisted of only one affinity chromatography step to capture the target protein. the purified cholesterol o ...201121420915
novel bacterial strains for the removal of microcystins from drinking water.microcystins (mc) and nodularin (nod) are common contaminants of drinking water around the world and due to their significant health impact it is important to explore suitable approaches for their removal. unfortunately, these toxins are not always removed by conventional water treatments. one of the most exciting areas that hold promise for a successful and cost effective solution is bioremediation of microcystins. recent work resulted in successful isolation and characterisation of 10 novel ba ...201121436548
algicidal effects of a novel marine actinomycete on the toxic dinoflagellate alexandrium tamarense.a marine actinomycete strain bs01 with algicidal activity to the toxic dinoflagellate, alexandrium tamarense, was isolated from xiamen bay, china. sequence analysis of 16s rdna demonstrates that bs01 is closely related to the genus brevibacterium of actinomycetales. bs01 exhibited algicidal activity in an indirect manner. additional organic nutrients, but not algal-derived dissolved organic matter, were necessary for the synthesis of yet unidentified algicidal compounds (molecular weight less th ...201121476052
development of a cell immobilization technique for the conversion of polysialogangliosides to monosialotetrahexosylganglioside.context: monosialotetrahexosylganglioside (gm(1)) prepared from the brain of pig or bovine is an effective clinical drug in the treatment of different nervous system diseases. generally, polysialogangliosides are transformed into gm(1) by enzymic or chemical hydrolysis due to the very poor level of natural gm(1). objective: to continuously obtain gm(1) by cell immobilization in a packed-bed reactor. materials and methods: brevibacterium casei, which is gram-positive bacteria belonging to the ord ...201121500968
evaluation of the impact of dna extraction methods on bac bacterial community composition measured by denaturing gradient gel electrophoresis.aims:  the impact of dna extraction methods on biological activated carbon (bac) dna yield and bacterial community was evaluated. methods and results:  three different dna extraction methods were compared: method a, method b and method c. method c with ultrasonic pretreatment improved cell lysis efficiency (from 34% to 87%) and dna yield [from 10·58 μg g(-1) (dry wt) of carbon to 21·42 μg g(-1) (dry wt) of carbon]. denaturing gradient gel electrophoresis profiles obtained by method c recovered t ...201121535041
endolysin of bacteriophage bfk20: evidence of a catalytic and a cell wall binding domain.a gene product of orf24' was identified on the genome of corynephage bfk20 as a putative phage endolysin. the protein of endolysin bfk20 (gp24') has a modular structure consisting of an n-terminal amidase_2 domain (gp24cd) and a c-terminal cell wall binding domain (gp24bd). the c-terminal domain is unrelated to any of the known cell wall binding domains of phage endolysins. the whole endolysin gene and the sequences of its n-terminal and c-terminal domains were cloned; proteins were expressed in ...201121592196
surface microbial consortia from livarot, a french smear-ripened cheese.the surface microflora (902 isolates) of livarot cheeses from three dairies was investigated during ripening. yeasts were mainly identified by fourier transform infrared spectroscopy. geotrichum candidum was the dominating yeast among 10 species. bacteria were identified using biotype 100 strips, dereplicated by repetitive extragenic palindromic pcr (rep-pcr); 156 representative strains were identified by either box-pcr or (gtg)(5)-pcr, and when appropriate by 16s rdna sequencing and sds-page an ...201121815832
native aortic valve endocarditis caused by brevibacterium epidermidis in an immunocompetent patient.although brevibacterium species used to be considered as nonpathogenic microorganisms until recently, it seems that they can cause a wide variety of clinical diseases by acting mostly as opportunistic pathogens. the present case is the second reported case of infective endocarditis by brevibacterium species; however, it is the first reported infected native aortic valve in an immunocompetent patient.201121681068
improvement of l: -valine production at high temperature in brevibacterium flavum by overexpressing ilvebn(r)c genes.brevibacterium flavum atcc14067 was engineered for l: -valine production by overexpression of different ilv genes; the ilvebn(r)c genes from b. flavum nv128 provided the best candidate for l: -valine production. in traditional fermentation, l: -valine production reached 30.08 ± 0.92 g/l at 31°c in 72 h with a low conversion efficiency of 0.129 g/g. to further improve the l: -valine production and conversion efficiency based on the optimum temperatures of l: -valine biosynthesis enzymes (above 35 ...201121706252
improvement of the thermostability and enzymatic activity of cholesterol oxidase by site-directed mutagenesis.site-directed mutagenesis was applied to enhance the thermostability and enzymatic activity of cholesterol oxidase (chox) isolated from brevibacterium sp. three amino acid residues (q153e, f128l, and s143h) located near the fad-binding site of the enzyme were substituted based on structural analysis. the specific activity of the two-sites mutant q153e/f128l increased by 11.6% and the relative activity increased by 47% when grown for 2-áh at 50-¦c. this mutant is a potential industrial strain for ...201121701916
Could petroleum biodegradation be a joint achievement of aerobic and anaerobic microrganisms in deep sea reservoirs?ABSTRACT: Several studies suggest that petroleum biodegradation can be achieved by either aerobic or anaerobic microorganisms, depending on oxygen input or other electron acceptors and appropriate nutrients. Evidence from in vitro experiments with samples of petroleum formation water and oils from Pampo Field indicate that petroleum biodegradation is more likely to be a joint achievement of both aerobic and anaerobic bacterial consortia, refining our previous observations of aerobic degradation. ...201122196374
Dynamics and diversity of phosphate mineralizing bacteria in the coral reefs of Gulf of Mannar.Phosphatase Producing Bacteria (PPB) and Inorganic Phosphate Solubilizing Bacteria (IPSB) are important to reef nutrition. These microbes and phosphate concentration play a significant role in the productivity of coral reef ecosystems. A study was conducted in Gulf of Mannar coral reef ecosystem to understand the diversity of these groups of bacteria and their competence in mineralizing the phosphate. The PPB isolates were identified under six genera i.e. Bacillus, Pseudomonas, Micrococcus, Vibr ...201121953521
the fate of indigenous microbiota, starter cultures, escherichia coli, listeria innocua and staphylococcus aureus in danish raw milk and cheeses determined by pyrosequencing and quantitative real time (qrt)-pcr.the purpose of this work was to study the bacterial communities in raw milk and in danish raw milk cheeses using pyrosequencing of tagged amplicons of the v3 and v4 regions of the 16s rdna and cdna. furthermore, the effects of acidification and ripening starter cultures, cooking temperatures and rate of acidification on survival of added escherichia coli, listeria innocua and staphylococcus aureus in cheeses at different stages of ripening were studied by pyrosequencing and quantitative real tim ...201122154239
isolation and characterization of lipase-producing bacteria in the intestine of the silkworm, bombyx mori, reared on different forage.abstract the silkworm, bombyx mori l. (lepidoptera: bombycidae), an oligophagous insect that mainly feeds on mulberry leaves, is susceptible to entomopathogen infection when reared with tricuspid cudrania leaves. a total of 56 dominant bacterial strains, classified into 12 phylotypes based on bacteriological properties and analysis of 16s rrna genes, were isolated from the intestine of the fourth and fifth instar silkworm larvae. ten and seven phylotypes exist in the intestine of the silkworm ...201122243438
brevibacterium casei as a cause of brain abscess in an immunocompetent patient.coryneform bacteria belonging to the genus brevibacterium have emerged as opportunistic pathogens. of the nine known species of brevibacterium isolated from human clinical samples, brevibacterium casei is the most frequently reported species from clinical specimens. we report the first case of b. casei brain abscess in an immunocompetent patient successfully treated by surgery and antimicrobial therapy.201122012007
preparation and characterization of affinity sorbents based on isoalloxazine-like ligands for separation of flavoenzymes.affinity ligands for flavoenzymes were synthesized based on the natural structure of flavo-coenzymes. two typical flavoenzymes, cholesterol oxidase from brevibacterium sp. and xanthine oxidase from bovine milk, were employed as standard enzymes. fluorescent probes were synthesized from eight isoalloxazine-like chemicals and 5-aminofluorescein. probe-enzyme interactions were analyzed via fluorescence spectra. chemicals with high binding abilities to flavoenzymes were coupled with sepharose throug ...201121936055
characterization in the archaeological excavation site of heterotrophic bacteria and fungi of deteriorated wall painting of herculaneum in italy.microbiological characterization of frescos in four different locations (collegio degli augustali, casa del colonnato tuscanico, casa dello scheletro and casa del gran portale) of excavation sites of herculaneum was carried out. the use of infrared thermography allowed detecting sample points on frescos with greatest moisture not visible to the naked eye, resulting in structural damage. the microclimatic conditions provided perfect habitat for bacteria and fungi, particularly of spore forming an ...201121882662
indigenous soil bacteria with the combined potential for hydrocarbon consumption and heavy metal resistance.introduction: transconjugant bacteria with combined potential for hydrocarbon utilization and heavy metal resistance were suggested by earlier investigators for bioremediation of soils co-contaminated with hydrocarbons and heavy metals. the purpose of this study was to offer evidence that such microorganisms are already part of the indigenous soil microflora. methods: microorganisms in pristine and oily soils wer ...201121948132
S-methyl thioesters are produced from fatty acids and branched-chain amino acids by brevibacteria: focus on L: -leucine catabolic pathway and identification of acyl-CoA intermediates.Despite their importance as potent odors that contribute to the aroma of numerous cheeses, S-methyl thioesters formation pathways have not been fully established yet. In a first part of our work, we demonstrated that Brevibacterium antiquum and Brevibacterium aurantiacum could produce S-methyl thioesters using short-chain fatty acids or branched-chain amino acids as precursors. Then, we focused our work on L: -leucine catabolism using liquid chromatography tandem mass spectrometry and gas chroma ...201121858675
Biologically synthesized fluorescent CdS NPs encapsulated by PHB.Here an attempt was made to biologically synthesize fluorescent cadmium sulfide nanoparticles and to immobilize the synthesized nanoparticles in PHB nanoparticles. The present study uses Brevibacterium casei SRKP2 as a potential producer for the green synthesis of CdS nanoparticles. Biologically synthesized nanoparticles were characterized and confirmed using electron microscopy and XRD. The size distribution of the nanoparticles was found to be 10-30 nm followed by which the consequence of time ...201122112944
evaluation of the microbial flora found in woodwind and brass instruments and their potential to transmit diseases.previous studies of dental devices (toothbrushes, dentures, and protective athletic mouthguards) have demonstrated microbial contamination of these devices and possible transmission of infectious diseases to the users. since woodwind and brass instruments come into intimate contact with the musician's oral cavity and often are passed from student to student without sanitization, the question arises as to whether these instruments are contaminated and can transmit microbial diseases. the purpose ...201121903519
degradation of ochratoxin a by brevibacterium species.the ability to degrade ochratoxin a was studied in different bacteria with a well-known capacity to transform aromatic compounds. strains belonging to rhodococcus, pseudomonas, and brevibacterium genera were grown in liquid synthetic culture medium containing ochratoxin a. brevibacterium spp. strains showed 100% degradation of ochratoxin a. ochratoxin α was detected and identified by high-performance liquid chromatography-mass spectrometry (hplc-ms) as a degradation product in the cell-free supe ...201121892825
genome sequence of corynebacterium glutamicum atcc 14067, which provides insight into amino acid biosynthesis in coryneform bacteria.we report the genome sequence of corynebacterium glutamicum atcc 14067 (once named brevibacterium flavum), which is useful for taxonomy research and further molecular breeding in amino acid production. preliminary comparison with those of the reported coryneform strains revealed some notable differences that might be related to the difficulties in molecular manipulation.201222247536
lc-ms method for screening unknown microbial carotenoids and isoprenoid quinones.the structure of secondary metabolites from microorganisms provides a useful tool for microbial characterization and chemotaxonomic classification. microbial isoprenoid quinones, for example, are well described and used to distinguish among photosynthetic microorganism groups. in addition, isoprenoid quinones can also be found, together with carotenoids, in non-photosynthetic microorganisms. the aim of the present study was to develop a lc-ms/ms method which can analyze and identify these microb ...201222036764
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