Publications

action pattern of polysaccharide lyases on glycosaminoglycans.	the action pattern of polysaccharide lyases on glycosaminoglycan substrates was examined using viscosimetric measurements and gradient polyacrylamide gel electrophoresis (page). heparin lyase i (heparinase, ec 4.2.2.7) and heparin lyase ii (no ec number) both acted on heparin in a random endolytic fashion. heparin lyase ii showed an ideal endolytic action pattern on heparan sulphate, while heparin lyase i decreased the molecular weight of heparan sulphate more slowly. heparin lyase iii (hepariti ...	1994	7949654
heterotrophic bacterial populations in the mineral waters of thermal springs in spain.	the microbiological quality and heterotrophic bacterial populations of 26 thermal mineral water springs in spain were studied. in most of the springs the number of viable aerobes was less than 10(3) cfu ml-1 and the number of sporulated bacteria less than 10(2) cfu ml-1. no significant differences were found in the counts obtained with plate count agar (pca) and pca diluted 1:10 and incubated at 22 degrees, 37 degrees and 45 degrees c. total coliforms were found in 14 springs, faecal streptococc ...	1994	7989265
secretion of a temporally controlled cell-associated protein in arthrobacter aureus: production of the protein and inactivation of its structural gene.	arthrobacter aureus secretes 2 proteins of 22 and 100 kda (p22 and p100, respectively). p22 is a protease, while p100, of unknown function, is associated with the cells before being released into the medium. an immunologically related p100 protein was also found in the culture supernatant of a. ureafasciens. despite the fact that production of p22 and p100 proteins is induced by growth in the presence of bovine serum albumin, their synthesis is not always induced coordinately. inactivation of th ...	1994	7997642
differential reactivity of two types of n-glycolyneuraminic acid dimers toward enzymatic and nonenzymatic hydrolysis of their interketosidic linkages.	the kinetics of acid- and sialidase-catalyzed hydrolysis of the interketosidic linkages of two different disialic acids, neu5gc alpha 2-->5-oglycolylneu5gc and neu5gc alpha 2-->8neu5gc, were studied. the former sequence was recently identified in the polysialic acid chains of a sialic acid-rich glycoprotein isolated from the egg jelly coat of two different species of sea urchins, and the latter was previously found in the cortical alveolar-derived polysialoglycoprotein from rainbow trout eggs. a ...	1994	7999128
molecular cloning and expression of an isomalto-dextranase gene from arthrobacter globiformis t6.	the gene encoding an extracellular isomalto-dextranase, designated imd, was isolated from the chromosomal dna of arthrobacter globiformis t6 and cloned and expressed in escherichia coli. a single open reading frame consisting of 1,926 base pairs that encoded a polypeptide composed of a signal peptide of 39 amino acids and a mature protein of 602 amino acids (m(r), 65,900) was found. the primary structure had no significant homology with the structures of any other reported carbohydrases, includi ...	1994	8002600
pcr protocol- and inulin catabolism-based differentiation of inulinolytic soil bacteria.	bacteria collected from rotting dahlia tubers, instead of degrading inulin to d-fructose, preferentially formed the known dfa iii (beta-2.1': alpha-2',3 difructofuranose anhydride), inulobiose, higher inulo-oligosaccharides, and exoheteropolysaccharides. owing to the morphological and gram staining variability, the bacterial isolates designated ylw and crm were examined to differentiate them from a reference strain arthrobacter ureafaciens. the comparative analyses were whole dna random amplific ...	1994	8010763
purification and characterization of a pyrrole-2-carboxylate oxygenase from arthrobacter strain py1.	pyrrole-2-carboxylate oxygenase was purified 8.2-fold to homogeneity from arthrobacter strain py1 grown on pyrrole-2-carboxylate as sole carbon, nitrogen, and energy source. fad and dithioerythritol had to be present during the purification procedure to stabilize the enzyme activity. the molecular mass of the pyrrole-2-carboxylate oxygenase was about 160 kda by gel filtration chromatography and native gradient page, only one polypeptide of about 60 kda was present after sds-page. the fad content ...	1994	8011178
kinetic characterization of tyramine oxidase of arthrobacter species.	amine oxidases (ec 1.4.3) represent a rather nebulous group of proteins with relative narrow substrate and inhibitor specificities. several different enzymes fall into these amine oxidase classes and the classification of some of them still remains ambiguous. kinetic and physico-chemical properties of tyramine oxidase of arthrobacter sp. were investigated to decide if this enzyme belongs to the flavin containing tyramine oxidase class (ec 1.4.3.9) or if it is more related to another amine oxidas ...	1994	8038724
regioselective transglycosylation in the synthesis of oligosaccharides: comparison of beta-galactosidases and sialidases of various origins.	n-acetyl-lactosamine(beta-d-gal p-(1-->4)-d-glc pnac) was synthesized regioselectively with the aid of the transglycosylation activity of beta-galactosidase isolated from diplococcus pneumoniae using p-nitrophenyl beta-d-galactopyranoside as the donor. also, transglycosylation of the sialyl group in an alpha-(2-->8)-linked sialic acid dimer or p-nitrophenyl glycoside of sialic acid to n-acetyl-lactosamine was performed using sialidases of various origins. when sialidase from clostridium perfring ...	1994	8039189
glycosphingolipid component profiles of human gliomas correlate with histological tumour types: analysis of inter-individual and tumour-regional distribution.	three types of glycosphingolipid (gsl) component profiles have been established for human intracranial gliomas. gsl-type i shows only glac- and lacto-series-sialoglycolipids. type ii consist of glac- and gtri-gangliosides, whereas only gsl-type iii contains sulphatide and, as a major neutral glycolipid, galactocerebroside, besides gangliosides of the glac-, gtri-, and gtet-families. whole gliomas of malignancy grading i/ii, iii and iv, display gsl-types iii, ii, and i, respectively. thus, the gs ...	1994	8042551
[mossbauer data on the effect of nad.h on the state of iron in bacteria].	the state of fe of bacterial cultures of different systematic positions (bacillus megaterium, bacillus polymyxa, pseudomonas putida, pseudomonas fluorescens, alcaligenes faecalis, arthrobacter siderocapsulatus) grown on the medium containing fe(iii) citrate (up to 100 mg/l) with additional or without nad.h was studied. the samples were in damp air-dry, second moistened, dried at 383 k states. spectra have been obtained at 290 k and 100-200 k. the studied microorganisms have two types of atoms of ...	1994	8043633
detection, isolation, and characterization of siderophores.		1994	8057905
roles of bacterial attachment and spontaneous partitioning in the biodegradation of naphthalene initially present in nonaqueous-phase liquids.	the mineralization by an arthrobacter sp. of naphthalene initially dissolved in di(2-ethylhexyl)phthalate exhibited a slow phase followed by a rapid phase. triton x-100, which inhibited cell attachment, prevented the onset of the second phase. triton x-100 increased the extent of mineralization of naphthalene initially present in 2,2,4,4,6,8,8-heptamethylnonane. cells attached to the interface mineralized the aromatic hydrocarbon at a rate four times higher than the rate of partitioning in the a ...	1994	8074534
identification of polysialic acid-containing glycoprotein in the jelly coat of sea urchin eggs. occurrence of a novel type of polysialic acid structure.	sea urchin eggs are surrounded by a gelatinous layer (called the jelly coat) that consists of mixture of fucoserich polysaccharides and sialic acid-rich glycoproteins. chemical and 500 mhz 1h nmr spectroscopic studies revealed for the first time the presence of a novel polysialic acid (polysia) structure in the jelly coat glycoproteins isolated from hemicentrotus pulcherrimus (designated polysia-gp(h)). the structure of the polysia chains was thoroughly characterized as (-->5-oglycolyl-neu5gc al ...	1994	8077223
generation of the topa quinone cofactor in bacterial monoamine oxidase by cupric ion-dependent autooxidation of a specific tyrosyl residue.	the quinone of 2,4,5-trihydroxyphenylalanine (topa), recently identified as the covalently bound redox cofactor in copper amine oxidases, is encoded by a specific tyrosine codon. to elucidate the mechanism of its formation, the recombinant phenylethylamine oxidase of arthrobacter globiformis has been overproduced in escherichia coli and purified in a cu(2+)-deficient form. the inactive precursor enzyme thus obtained was dramatically activated upon incubation with cu2+, concomitantly with the for ...	1994	8082796
biodegradation of p-nitrophenol via 1,2,4-benzenetriol by an arthrobacter sp.	the degradation of p-nitrophenol (pnp) by moraxella and pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. the resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. we have isolated a strain of an arthrobacter sp., js443, capable of degrading pnp with stoichiometric release of nitrite. during induction of the enzymes required for growth on pnp, 1,2,4-benzenetriol was identified as an intermediate by gas chromatography-mass spec ...	1994	8085840
characterization of psychrotrophic microorganisms producing beta-galactosidase activities.	investigations of psychrotrophic microorganisms have been limited even though the dominant environment of the earth is cold and enzymes with high activities at low temperatures could have commercial uses. we have isolated and characterized three psychrotrophic strains with beta-galactosidase activities. the isolates, b7, d2, and d5, were gram-positive, catalase-positive, obligate aerobes. cells observed with a scanning electron microscope appeared as rods during the early stages of growth but be ...	1994	8117071
characterization of an endoserine protease secreted by arthrobacter aureus.	a 22-kda endoserine protease secreted by an arthrobacter aureus strain was identified. the optimal temperature for this protease was 70 degrees c. protease production was maximal at the end of the exponential growth phase, and the protease was induced by amino acids and peptides and repressed by carbon sources.	1994	8117088
occurrence and expression of cspa, a cold shock gene, in antarctic psychrotrophic bacteria.	the homologue of cold shock gene cspa of escherichia coli was detected in various isolates of antarctic psychrotrophs representing both gram-positive and gram-negative bacteria. the northern hybridization study indicated that the transcript size of cspa in the psychrotrophic gram-positive bacterium arthrobacter protophormiae and gram-negative pseudomonas fluorescens was similar to that of e. coli and that the cspa homologues in these two psychrotrophs were expressed constitutively at a low level ...	1994	8132155
determination of sialic acids.		1994	8139495
cloning and sequencing of phenylethylamine oxidase from arthrobacter globiformis and implication of tyr-382 as the precursor to its covalently bound quinone cofactor.	the gene of arthrobacter globiformis encoding a quinoprotein, phenylethylamine oxidase, has been cloned and sequenced. in the deduced amino acid sequence comprising 638 residues is a tetrapeptide sequence, asn-tyr-asp-tyr, which has been found to be highly conserved in other copper amine oxidase. mutation of the former tyr (tyr-382) of the recombinant enzyme into phe resulted in the complete loss of catalytic activity and disappearance of the quinone compound that is specifically detected in the ...	1994	8147851
evidence for the interference of aluminum with bacterial porphyrin biosynthesis.	aluminum (0.74 mm) was found to retard bacterial growth, and enhance porphyrin formation and excretion in arthrobacter aurescens rs-2. coproporphyrin iii was shown to be the main porphyrin excreted by aluminum-exposed a. aurescens rs-2 cultures and by rs-2 cultures grown under anoxic conditions. synthesis and excretion of porphyrins in a. aurescens rs-2 increased in a dose-dependent manner when the bacteria were exposed to increasing aluminum concentrations. incubation of a. aurescens rs-2 with ...	1994	8148615
analysis of low-molecular mass products from biosolubilized coal.	a relatively simple, rapid sample preparation method has been developed for analysis of low-molecular mass compounds present in soluble coal products generated by microbial coal solubilizing agents. acidification of the sample followed by direct extraction into hexanes is coupled with gas chromatography/mass spectrometry analysis for characterization of the soluble coal products. characterization of the products can contribute to a more complete understanding of the solubilization processes invo ...	1994	8181700
the penicillin amidase of arthrobacter viscosus (atcc 15294).	the nucleotide (nt) sequence of the gene encoding penicillin g amidase (pa) of arthrobacter viscosus strain atcc 15,294 was determined. the sequence contained an open reading frame of 2406 nt with a g+c content of 37%. the deduced amino-acid sequence shows significant homology with other so far identified beta-lactam amidases of gram- bacteria.	1994	8200542
chemical structures of hetero-oligosaccharides produced by arthrobacter sp. k-1 beta-fructofuranosidase.	the structures of hetero-oligosaccharides obtained by the action of transglycosylation of arthrobacter sp. k-1 beta-fructofuranosidase, using sucrose as the fructosyl donor, and several mono- and di-sacchrides as the acceptors were investigated. the main transfer products to most reducing mono- and di-sacchrides were non-reducing oligosaccharides with a fructosyl residue linked to a hemiacetal hydroxyl group. in the presence of l-sorbose, the enzyme produced 2-o-beta-d-fructofuranosyl-alpha-l-so ...	1994	7764539
purification of inulin fructotransferase (dfa i-producing) from arthrobacter sp. mci2493 and production of dfa i from inulin by the enzyme.	an extracellular enzyme that produces di-d-fructofuranose-2',1;2,1' dianhydride from inulin was purified from the culture broth of arthrobacter sp. mci2493. the molecular weight of the enzyme was 40,000 by gel filtration and sds polyacrylamide gel electrophoresis. the enzyme had maximum activity at ph 6.0 and 50 degrees c. using this purified enzyme, 100 g/liter inulin was converted into 60 g/liter of dfa i, nystose, and 1-f-fructofuranosyl-nystose after incubation for 30 h.	1994	7764697
levoglucosan dehydrogenase involved in the assimilation of levoglucosan in arthrobacter sp. i-552.	a levoglucosan (1,6-anhydro-beta-d-glucopyranose)-using bacterium, isolated from soil, was identified. it was shown to belong to the genus arthrobacter and tentatively named arthrobacter sp. i-552. a novel enzyme catalyzed the dehydrogenation of levoglucosan to form 1,6-anhydro-beta-d-ribo-hexopyranos-3-ulose (3-keto levoglucosan), using nad+ as an electron acceptor, i.e. nad+: 1,6-anhydro-beta-d-glucopyranose oxidoreductase (trivial name: levoglucosan dehydrogenase). this enzyme was purified an ...	1994	7765713
construction of a new host-vector system in arthrobacter sp. and cloning of the lipase gene.	arthrobacter sp. strain mis38 was transformed with a shuttle vector containing the kanamycin resistant gene kan (derived from tn5) by an electroporation method. this shuttle vector is from brevibacterium lactofermentum and escherichia coli, pulrs8. the following optimal condition of electroporation was determined. a square wave pulse of 1 kv/cm electric field strength for 0.5 ms duration yielded 3 x 10(5) transformants/micrograms plasmid dna. the number of transformants increased with the amount ...	1994	7765770
biotransformation of hydrocortisone into prednisolone.		1994	7519301
phylogenetic analysis of species of the meso-diaminopimelic acid-containing genera brevibacterium and dermabacter.	16s rrna gene sequencing studies were performed on dermabacter hominis and four meso-diaminopimelic acid-containing species of the genus brevibacterium. phylogenetic analysis revealed a close association between dermabacter hominis and representatives of the lysine-containing genera arthrobacter, micrococcus, and renibacterium. by contrast, the genus brevibacterium formed a distinct line of descent within the high-guanine-plus-cytosine-containing actinomycetes, displaying no specific affinity wi ...	1994	7520745
characterization of a psychrotrophic arthrobacter gene and its cold-active beta-galactosidase.	enzymes with high specific activities at low temperatures have potential uses for chemical conversions when low temperatures are required, as in the food industry. psychrotrophic microorganisms which grow at low temperatures may be a valuable source of cold-active enzymes that have higher activities at low temperatures than enzymes found for mesophilic microorganisms. to find cold-active beta-galactosidases, we isolated and characterized several psychrotrophic microorganisms. one isolate, b7, is ...	1994	7811090
the sialic acid-containing lipopolysaccharides of salmonella djakarta and salmonella isaszeg (serogroup o: 48): chemical characterization and reactivity with a sialic acid-binding lectin from cepaea hortensis.	lipopolysaccharides (lps) of salmonella djakarta and salmonella isaszeg, as well as of a spontaneous r-mutant of s. djakarta were investigated as to their content in neuraminic acid (neu) and its individual linkage. the two salmonella serovars both belong to the o:48 serogroup of salmonella, but to two different subgroups. lps of both s-forms contained high amounts of neu, although in different quantities, whereas the r-form was completely devoid of it. methylation analysis indicated that neu is ...	1994	7812267
structural analysis and molybdenum-dependent expression of the pao1-encoded nicotine dehydrogenase genes of arthrobacter nicotinovorans.	the genes of nicotine dehydrogenase (ndh) were identified, cloned and sequenced from the catabolic plasmid pao1 of arthrobacter nicotinovorans. in immediate proximity to this gene cluster is the beginning of the 6-hydroxy-l-niotine oxidase (6-hlno) gene. ndh is composed of three subunits (a, b and c) of m(r) 30,011, 14,924 and 87,677. it belongs to a family of bacterial hydroxylases with a similar subunit structure; they have molybdopterin dinucleotide, fad and fe-s clusters as cofactors. here t ...	1994	7815950
isomerization of trans-2,delta 5-dienoyl-coa's to delta 3,delta 5-dienoyl-coa's in the beta-oxidation of delta 5-unsaturated fatty acids.	the nadph-dependent reduction pathway for the metabolism of delta 5-unsaturated fatty acids involves the isomerization of trans-2,delta 5-dienoyl-coa, initially formed from the dehydrogenation of delta 5-enoyl-coa, to isomeric delta 3,delta 5-dienoyl-coa. the latter intermediates were then isomerized to trans-2,trans-4-dienoyl-coa, which then follows the nadph-dependent pathway mediated by 2,4-dienoyl-coa reductase. the isomerization from trans-2,delta 5-dienoyl-coa to delta 3,delta 5-dienoyl-co ...	1995	7819236
synthesis of neoglycoproteins using oligosaccharide-transfer activity with endo-beta-n-acetylglucosaminidase.	we describe a novel method for the enzymatic synthesis of neoglycoproteins. endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) had high levels of transglycosylation activity. the enzyme activity of endo-a was markedly increased by adding 4-l-aspartyl-glycosylamine (glcnac-asn) to the reaction mixture. digesting (man)6(glcnac)2 with the enzyme in the presence of glcnac-asn gave a mixture of hydrolytic ((man)6glcnac) and transglycosylic ((man)6(glcnac)2-asn) products. by me ...	1995	7852391
a manganese-dependent dioxygenase from arthrobacter globiformis cm-2 belongs to the major extradiol dioxygenase family.	almost all bacterial ring cleavage dioxygenases contain iron as the catalytic metal center. we report here the first available sequence for a manganese-dependent 3,4-dihydroxyphenylacetate (3,4-dhpa) 2,3-dioxygenase and its further characterization. this manganese-dependent extradiol dioxygenase from arthrobacter globiformis cm-2, unlike iron-dependent extradiol dioxygenases, is not inactivated by hydrogen peroxide. also, ferrous ions, which activate iron extradiol dioxygenases, inhibit 3,4-dhpa ...	1995	7868595
copper/topa quinone-containing histamine oxidase from arthrobacter globiformis. molecular cloning and sequencing, overproduction of precursor enzyme, and generation of topa quinone cofactor.	the gene coding for histamine oxidase has been cloned and sequenced from a coryneform bacterium arthrobacter globiformis. the deduced amino acid sequence consists of 684 residues with a calculated molecular mass of 75,109 daltons and shows a high overall identity (58%) with that of phenethylamine oxidase derived from the same bacterial strain. although the sequence similarities are rather low when compared with copper amine oxidases from other organisms, the consensus asn-tyr-asp/glu sequence, i ...	1995	7876243
active site analysis and stabilization of sarcosine oxidase by the substitution of cysteine residues.	two cysteine residues (c-265 and c-318) in the putative hydrophilic regions of sarcosine oxidase were substituted by using site-directed mutagenesis. since the mutant with the c-to-s mutation at position 318 (c318s) lost the enzyme activity, c-318 (conserved among sarcosine oxidases) is most likely a part of the active site. c265s, c265a, c265d, and c265r showed nearly the same enzymatic properties as those of the wild type. however, they were much more stable than the wild type in the presence ...	1995	7887617
isolation of insertion elements from gram-positive brevibacterium, corynebacterium and rhodococcus strains using the bacillus subtilis sacb gene as a positive selection marker.	the sacb gene of bacillus subtilis was successfully applied in various arthrobacter, brevibacterium, corynebacterium and rhodococcus strains for the isolation of transposable elements. three different insertion sequence (is) elements entrapped in sacb were isolated. the is elements is-bl and is-cg isolated from brevibacterium lactofermentum and corynebacterium glutamicum, respectively, were found to be similar in size (1.45 kb) and generated target duplications of 8 bp. their inverted repeats sh ...	1995	7896070
transformation of synechococcus with a gene for choline oxidase enhances tolerance to salt stress.	choline oxidase, isolated from the soil bacterium arthrobacter globiformis, converts choline to glycinebetaine (n-trimethylglycine) without a requirement for any cofactors. the gene for this enzyme, designated coda, was cloned and introduced into the cyanobacterium synechococcus sp. pcc 7942. the coda gene was expressed under the control of a strong constitutive promoter, and the transformed cells accumulated glycinebetaine at intracellular levels of 60-80 mm. consequently the cells acquired tol ...	1995	8555454
isolation and partial characterization of yeast mannan hydrolysing enzymes from bacterial isolates.	a range of aerobic, mesophilic bacterial strains capable of producing extracellular alpha-mannanase and alpha-mannosidase enzymes were isolated from various natural habitats using yeast cell wall material as the selective medium. five of them were capable of producing extracellular alpha-mannosidase and alpha-mannanase on yeast mannan as the sole carbon substrate, and they were tentatively identified to the genus level as arthrobacter, streptomyces, pseudomonas, bacillus and acinetobacter. among ...	1995	8577261
purification and characterization of the 3-ketosteroid-delta 1-dehydrogenase of arthrobacter simplex produced in streptomyces lividans.	the 3-ketosteroid-delta 1-dehydrogenase (ks1dh) gene of arthrobacter simplex ifo12069 cloned in streptomyces lividans was overexpressed, resulting in production of the enzyme both extracellularly and intracellularly. the enzyme was purified by ammonium sulfate fractionation and chromatographies using deae-toyopearl, butyl-toyopearl and toyopearl hw55s from the supernatant of culture broth and cell-free extracts of s. lividans, and both preparations showed the same characteristics. the n-terminal ...	1995	8586617
a pao1-encoded molybdopterin cofactor gene (moaa) of arthrobacter nicotinovorans: characterization and site-directed mutagenesis of the encoded protein.	a gene homologous to moaa, the gene responsible for the expression of a protein involved in an early step in the synthesis of the molybdopterin cofactor of escherichia coli, was found to be located 2.7-kb upstream of the nicotine dehydrogenase (ndh) operon on the catabolic plasmid pao1 of arthrobacter nicotinovorans. the moaa protein, containing 354 amino acids, migrated on an sds-polyacrylamide gel with an apparent molecular weight of 40,000, in good agreement with the predicted molecular weigh ...	1995	8588735
formation of polyhydroxylated isoflavones from the soybean seed isoflavones daidzein and glycitein by bacteria isolated from tempe.	five tempe-derived bacterial strains identified as micrococcus or arthrobacter species were shown to transform the soybean isoflavones daidzein and glycitein to polyhydroxylated isoflavones by different hydroxylation reactions. all strains converted glycitein and daidzein to 6,7,4'-trihydroxyisoflavone (factor 2) and the latter substrate also to 7,8,4'-trihydroxyisoflavone. three strains transformed daidzein to 7,8,3',4'-tetrahydroxyisoflavone and 6,7,3',4'-tetrahydroxyisoflavone. in addition, t ...	1995	8588745
comparison of 16s ribosomal rna genes in clavibacter michiganensis subspecies with other coryneform bacteria.	nearly complete sequences (97-99%) of the 16s rrna genes were determined for type strains of clavibacter michiganensis subsp. michiganensis, clavibacter michiganensis subsp. insidiosus, clavibacter michiganensis subsp. sepedonicus, and clavibacter michiganensis subsp. nebraskensis. the four subspecies had less than 1% dissimilarity in their 16s rrna genes. comparative studies indicated that the c. michiganensis subsp. shared relatively high homology with the 16s rrna gene of clavibacter xyli. fu ...	1995	8590406
secretory overproduction of arthrobacter simplex 3-ketosteroid delta 1-dehydrogenase by streptomyces lividans with a multi-copy shuttle vector.	the gene for 3-ketosteroid delta 1-dehydrogenase (ksdd) of arthrobacter simplex was expressed in streptomyces lividans and the secreted enzyme was overproduced by using a multi-copy shuttle vector composed of pij702 and puc19. deletional analysis of the recombinant plasmid showed that the entire coding sequence of the ksdd gene was located within a 7-kb segment of the chromosomal dna obtained from the enzyme-producing strain of a. simplex. when s. lividans carrying the recombinant plasmid was gr ...	1995	8590655
purification and properties of a novel enzyme, maltooligosyl trehalose synthase, from arthrobacter sp. q36.	arthrobacter sp. q36 produces a novel enzyme, maltooligosyl trehalose synthase, which catalyzes the conversion of maltooligosaccharide into the non-reducing saccharide, maltooligosyl trehalose (alpha-maltooligosyl alpha-d-glucoside) by intramolecular transglycosylation. the enzyme was purified from a cell-free extract to an electrophoretically homogeneous state by successive column chromatography on sepabeads fp-da13, deae-sephadex a-50, ultrogel aca44, and butyl-toyopearl 650m. the enzyme was s ...	1995	8611744
purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from arthrobacter sp. q36.	a novel enzyme, maltooligosyl trehalose trehalohydrolase from arthrobacter sp. q36 was purified from a cell-free extract to an electrophoretically pure state by successive column chromatography on sepabeads fp-da13, butyl-toyopearl 650m, deae-toyopearl 650s, and toyopearl hw-55s. the enzyme specifically catalyzed the hydrolysis of the alpha-1,4-glucosidic linkage that bound the maltooligosyl and trehalose moieties of maltooligosyl trehalose. the km of the enzyme for maltosyl trehalose, maltotrio ...	1995	8611745
structure of simusan, a new acidic exopolysaccharide from arthrobacter sp.	simusan, a major exopolysaccharide produced by an ethanol-utilizing arthrobacter sp. strain ce-17, contains d-glucose, d-mannose, d-galactose, l-rhamnose, d-glucuronic acid, and pyruvic acid in the ratios approximately 3:2:1:1:1:1 as well as o-acyl groups (presumably (presumably residues of acetic and palmitic acid). on the basis of chemical modifications of the polysaccharide, solvolysis with anhydrous hydrogen fluoride resulting in a penta- and an octa-saccharide fragment, smith degradation, a ...	1995	7697646
establishment of oil-degrading bacteria associated with cyanobacteria in oil-polluted soil.	a unique natural microbial cocktail with promising potential for remediating oil-polluted desert in the gulf region is reported. oil-degrading micro-organisms immobilized within dense cyanobacterial mats on oily coasts of the arabian gulf were successfully established in oil-contaminated sand. those micro-organisms biodegraded 50% of the oil within 10-20 weeks. nocardioforms belonging to the genus rhodococcus predominated in the first few weeks, but after 22 weeks pseudomonas spp. increased, sha ...	1995	7698954
spectroscopic studies on the mechanism of the topa quinone generation in bacterial monoamine oxidase.	electron paramagnetic resonance (epr), circular dichroism (cd), and optical absorption spectroscopies have been used to investigate the copper-dependent autoxidation process generating the 6-hydroxydopa (topa) quinone cofactor in the recombinant phenethylamine oxidase from arthrobacter globiformis. the cupric ion bound to the copper/topa quinone-less, inactive enzyme is first reduced to cu(i), as inferred from the spectroscopic features observed under strictly anaerobic conditions. cu(i) is also ...	1995	7718554
analysis of a novel gene and beta-galactosidase isozyme from a psychrotrophic arthrobacter isolate.	we have characterized a new psychrotrophic arthrobacter isolate which produces beta-galactosidase isozymes. when dna from this isolate was transformed into an escherichia coli host, we obtained three different fragments, designated 12, 14, and 15, each encoding a different beta-galactosidase isozyme. the beta-galactosidase produced from fragment 12 was of special interest because the protein subunit was smaller (about 71 versus 116 kda) than those typically encoded by the lacz family. the isozym ...	1995	7721689
inactivation of pathogens during aerobic and anaerobic treatments at low temperatures.		1995	7749283
isolation and identification of bacteria from activated sludge purifying petroleum wastewaters.	several strains growing well in minimal media with 500 and 1000 mg/l of oil or phenol as a sole carbon source were isolated from activated sludge purifying petroleum waste waters and identified. five of the best growing strains classified as arthrobacter, pseudomonas, xanthomonas and enterobacter were selected and their capacity to remove petroleum components and phenol (in the oil fraction of petrochemical waste waters) was studied. the enzymatic activity of the strains, including respiration i ...	1995	8906933
tecogalan sodium inhibits corneal neovascularization induced by basic fibroblast growth factor.	the antiangiogenic effect of tecogalan sodium on corneal neovascularization was investigated. tecogalan sodium, a sulfated polysaccharide peptidoglycan complex isolated from an arthrobacter species, has been reported to inhibit angiogenesis induced by basic fibroblast growth factor (bfgf) as well as tumor angiogenesis related to kaposi's sarcoma. corneal neovascularization induced by bfgf was inhibited by tecogalan sodium in a dose-dependent manner. since bfgf is known to have a promoting effect ...	1995	8927305
a phylogenetic analysis of micro-organisms isolated from subsurface environments.	three methods were used to provide information on the identity and phylogenetic relatedness of 19 aerobic, chemoheterotrophic bacteria isolated from topsoil and deep subsurface sediments at a site in south carolina. these methods were (i) analysis of selected physiological traits, (ii) restriction endonuclease analysis (rea) of genomic dna, and (iii) analysis of 16s ribosomal rna sequences. when the 16s rrna sequences were compared with those for 12 standard strains, two topsoil isolates and six ...	1995	7536094
limonoate dehydrogenase from arthrobacter globiformis: the native enzyme and its n-terminal sequence.	bitter limonoids in citrus juice lower the quality and value of commercial juices. limonoate dehydrogenase converts the precursor of bitter limonin, limonoate a-ring lactone, to nonbitter 17-dehydrolimonoate a-ring lactone. this enzyme was isolated from arthrobacter globiformis cells by a combination of ammonium sulfate fractionation, cibacron blue affinity chromatography and deae ion exchange hplc. using this protocol a 428-fold purification of the enzyme was obtained. gel filtration hplc indic ...	1995	7546548
phylogenetic analysis of the genera cellulomonas, promicromonospora, and jonesia and proposal to exclude the genus jonesia from the family cellulomonadaceae.	the 16s rrna gene sequences of eight cellulomonas species, two promicromonospora species, and jonesia denitrificans were determined, and these sequences were compared with the sequences of about 50 representatives of the arthrobacter line of descent in the order actinomycetales. we found that in spite of its current assignment to the family cellulomonadaceae, j. denitrificans branches outside the radiation of this taxon and cannot be considered a member of it. the two promicromonospora species d ...	1995	7547283
reclassification of micrococcus agilis (ali-cohen 1889) to the genus arthrobacter as arthrobacter agilis comb. nov. and emendation of the genus arthrobacter.	phylogenetic evidence derived from a 16s ribosomal dna analysis indicated that the type strain of micrococcus agilis, dsm 20550 (= atcc 966 = ccm 2390), is less closely related to the type species of the genus micrococcus, micrococcus luteus, than to the type species of the genus arthrobacter, arthrobacter globiformis, and related arthrobacter species. the phylogenetic position of m. agilis is supported by the presence of peptidoglycan variation a3 alpha and by the presence of mk-9(h2) as the ma ...	1995	7547308
stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of arthrobacter globiformis in escherichia coli.	the gene coding for a novel esterase which stereoselectively hydrolyzes the (+)-trans (1r,3r) stereoisomer of ethyl chrysanthemate was cloned from arthrobacter globiformis sc-6-98-28 and overexpressed in escherichia coli. the cellular content of the active enzyme reached 33% of the total soluble protein in the recombinant e. coli jm105 cells and 5.6 g/liter of culture by high-density cell cultivation. the hydrolytic activity of the recombinant e. coli cells for ethyl chrysanthemate reached 605 m ...	1995	7574629
degradation of iprodione by a soil arthrobacter-like strain.	a bacterial strain able to transform iprodione was isolated from a fast iprodione-degrading soil by enrichment procedures. transformation was detected through 3,5-dichloroaniline production as measured by a rapid colorimetric method. the strain, ma6, was tentatively identified as an arthrobacter sp. when it was incubated with ma6 in a minimum mineral medium (ph 6.5), iprodione (8.8 mumol/liter) was transformed into two major metabolites that were identified by high-performance liquid chromatogra ...	1995	7574630
membrane hyperpolarisation by valinomycin and its limitations for bacterial viability assessment using rhodamine 123 and flow cytometry.	the ionophore, valinomycin, was investigated as a possible means of bacterial viability assessment using the fluorescent membrane potential dye rhodamine 123. membrane hyperpolarisation in escherichia coli, pseudomonas fluorescens, enterobacter aerogenes and arthrobacter globiformis was examined during exponential growth and during stress by brief starvation in a high sodium, low potassium buffer using flow cytometric analysis of rhodamine 123 uptake. dye uptake was variable both between species ...	1995	7590182
sequence and expression of the bpdc1c2bade genes involved in the initial steps of biphenyl/chlorobiphenyl degradation by rhodococcus sp. m5.	the nucleotide (nt) sequence of the bpdc1c2bade genes which encode the first three enzymes in the biphenyl (bp) degradation pathway of gram+ rhodococcus sp. m5 (formerly arthrobacter m5) was determined. except for the ferredoxin component (bpdb) of the initial bp dioxygenase, the predicted amino acid (aa) sequences of the remaining proteins are found to be more closely related to the counterpart proteins (todc1c2bade) present in the toluene-degrader, pseudomonas putida f1, than those of three bp ...	1995	7590299
molecular cloning, expression in streptomyces lividans, and analysis of a gene cluster from arthrobacter simplex encoding 3-ketosteroid-delta 1-dehydrogenase, 3-ketosteroid-delta 5-isomerase and a hypothetical regulatory protein.	the arthrobacter simplex gene coding for 3-ketosteroid-delta 1-dehydrogenase, a key enzyme in the degradation of the steroid nucleus, was cloned in streptomyces lividans. nucleotide sequence analysis revealed that the gene for 3-ketosteroid-delta 1-dehydrogenase (ksdd) is clustered with at least two more genes possibly involved in steroid metabolism. upstream of ksdd, we found a gene, ksdr, encoding a hypothetical regulatory protein that shows homologies to kdgr, the negative regulator of pectin ...	1995	7596291
enhanced transglycosylation activity of arthrobacter protophormiae endo-beta-n-acetylglucosaminidase in media containing organic solvents.	the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) was enhanced by inclusion of organic solvents in the reaction mixture. in aqueous solution, the transglycosylation yield relative to starting substrate was 32% using man9glcnac2asn as donor and 0.5 m glcnac as acceptor. however, in the media containing 30% (v/v) acetone, dioxane, n,n-dimethylformamide, or dimethyl sulfoxide with 0.5 m glcnac as acceptor, the transglycosylation attained y ...	1995	7629071
synthesis of neoglycoconjugates by transglycosylation with arthrobacter protophormiae endo-beta-n-acetylglucosaminidase. demonstration of a macro-cluster effect for mannose-binding proteins.	the transglycosylation activity of endo-beta-n-acetylglucosaminidase from arthrobacter protophormiae (endo-a) can be enhanced dramatically by inclusion of organic solvent in the reaction mixture (see accompanying article; fan, j.-q., takegawa, k., iwahara, s., kondo, a., kato, i., abeygunawardana, c., and lee, y. c. (1995) j. biol. chem. 270, 17723-17729). this finding was extended to synthesis of important intermediates for preparation of neoglycoconjugates. when 0.2 m glcnac-o-(ch2)6nh2, glcna ...	1995	7629072
red cells bound to influenza virus n9 neuraminidase are not released by the n9 neuraminidase activity.	influenza virus neuraminidase (na) of the n9 subtype also possesses hemagglutinin activity and the hemagglutinating, or hemabsorbing (hb), site is distinct from the catalytic site. previous results suggested that the na was binding to sialic acid on the red cell surface, but we now report that the hb receptor is not sensitive to n9 influenza neuraminidase activity. cell lines that constitutively express n9 or n2 neuraminidase have been used to further investigate the specificity of red blood cel ...	1995	7645221
use of a polymerase-chain-reaction-amplified dna probe from pseudomonas putida to detect d-hydantoinase-producing microorganisms by direct colony hybridization.	pseudomonas putida strain dsm 84 produces n-carbamyl-d-amino acids from the corresponding d-5-monosubstituted hydantoins. the sequence of the d-hydantoinase gene from this strain (genbank accession number l24157) was used to develop a dna probe of 122 base pairs (bp) that could detect d-hydantoinase genes in other bacterial genera by dna and by colony hybridization. under conditions tolerating 32% mismatch, the probe was specific for all strains that expressed d-hydantoinase activity. these incl ...	1995	7766091
reaction mechanism for the conversion of 5-monosubstituted hydantoins to enantiomerically pure l-amino acids.	the specific conversion of d,l-5-monosubstituted hydantoins to optically pure l-amino acids by resting cells of arthrobacter sp. dsm 7330 has been evaluated. a new nonstereoselective hydantoinase from arthrobacter sp. dsm 7330 was isolated and characterized. when whole cells were tested, the conversion of d,l-5-methylthioethylhydantoin (d,l-5-mteh) led to the optically pure intermediate d-carbamoylmethionine (d-cm) and to the optically pure amino acid l-methionine. after purification of the hyda ...	1995	7785836
two-dimensional gel electrophoresis of ribosomal proteins as a novel approach to bacterial taxonomy: application to the genus arthrobacter.	ribosomal proteins from 22 strains of 15 different species belong to the genus arthrobacter were analyzed by an improved two-dimensional gel electrophoresis. electrophoretograms of ribosomal proteins from 15 type strains had species-specific patterns. similarity coefficients (sab values) of ribosomal proteins with mol. wt. of greater than about 20,000, among strains of the same species (dna relatedness values of more than 61%) were greater than 0.85, but the sab values among strains of different ...	1995	8520111
cellular location influences copper-dependent topaquinone formation for phenylethylamine oxidase expressed in escherichia coli k-12.	arthrobacter globiformis amine oxidase produced by escherichia coli cells grown in copper-depleted media was reported to undergo activation due to formation of its topaquinone cofactor in a copper-dependent autocatalytic reaction. likewise, a mutated e. coli amine oxidase located in the cytoplasm was reported to form topaquinone autocatalytically in an edta-sensitive reaction. here we show unequivocally that formation of an amine oxidase lacking topaquinone is primarily a consequence of the loca ...	1995	8522142
resonance raman spectroscopy of quinoproteins.		1995	8524145
spectrophotometric detection of topa quinone.		1995	8524159
cloning and nucleotide sequence of the inulin fructotransferase (dfa i-producing) gene of arthrobacter globiformis s14-3.	a gene encoding an inulin fructotransferase (dfa i-producing) [ec 2.4.1.200] from arthrobacter globiformis s14-3 was cloned and the nucleotides sequenced, for the first time. the sequence indicated that the native enzyme protein is composed of 392 amino acid residues. the native enzyme is an extracellar enzyme produced in the culture supernatant of a. globiformis s14-3, but the nucleotide sequence of the gene lacks a sequence for signal peptide for secretion. the 1.5-kb dna fragment encoding the ...	1995	8534968
formation of trehalose from maltooligosaccharides by a novel enzymatic system.		1995	8534970
development of a specific biotinylated dna probe for the detection of renibacterium salmoninarum.	a specific dna probe for the identification of renibacterium salmoninarum, the causative agent of bacterial kidney disease (bkd), was developed from one of 3 clones prs47, prs49, and prs26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. the biotinylated prs47/bamhi insert probe was tested on 3 dilutions of dna extracted from 3 strains of r. salmoninarum and from 1 strain each of arthrobacter protophormiae, aeromonas salmonicida, corynebacterium aquaticum, carnobacterium piscicola, listonella angui ...	1995	8548693
soil and sediment bacteria capable of aerobic nitrate respiration.	several laboratory strains of gram-negative bacteria are known to be able to respire nitrate in the presence of oxygen, although the physiological advantage gained from this process is not entirely clear. the contribution that aerobic nitrate respiration makes to the environmental nitrogen cycle has not been studied. as a first step in addressing this question, a strategy which allows for the isolation of organisms capable of reducing nitrate to nitrite following aerobic growth has been develope ...	1995	7487017
cloning, nucleotide sequencing, and expression of an opine dehydrogenase gene from arthrobacter sp. strain 1c.	the gene coding for opine dehydrogenase from arthrobacter sp. strain 1c was cloned onto plasmid pbluescript ks(-), and the nucleotide sequence of the 1,077-bp open reading frame consisting of 359 codons was identified as the odh gene. transformed escherichia coli cells overproduced nad+-dependent opine dehydrogenase under control of the promoter of the lac gene on pbluescript ks(-).	1995	7487048
selective recovery of streptosporangium fragile from soil by indirect immunomagnetic capture.	a polyclonal antibody raised to streptosporangium fragile spores reacted strongly and specifically with the immunizing strain and to a number of related species of streptosporangium, as determined by dot immunoblotting. an indirect immunomagnetic capture method was developed for the recovery of the target organism from sterile and non-sterile soil, using sheep anti-rabbit m-280 dynabeads. the effects of different soil blocking agents, antibody labelling concentrations and spore/dynabead capture ...	1995	7496526
nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems.	dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ...	1995	16535048
sulfur-specific microbial desulfurization of sterically hindered analogs of dibenzothiophene.	dibenzothiophenes (dbts) bearing alkyl substitutions adjacent to the sulfur atom, such as 4,6-diethyldibenzothiophene (4,6-dedbt), are referred to as sterically hindered with regard to access to the sulfur moiety. by using enrichment cultures with 4,6-dedbt as the sole sulfur source, bacterial isolates which selectively remove sulfur from sterically hindered dbts were obtained. the isolates were tentatively identified as arthrobacter species. 4,6-dedbt sulfone was shown to be an intermediate in ...	1995	16535189
stereoselective production of (+)-trans-chrysanthemic acid by a microbial esterase: cloning, nucleotide sequence, and overexpression of the esterase gene of arthrobacter globiformis in escherichia coli.	volume 61, no. 9, p. 3209, column 2, line 23: "l22165" should read "l22516." [this corrects the article on p. 3208 in vol. 61.].	1996	16535302
production of two extracellular alkaline phosphatases by a psychrophilic arthrobacter strain.	we surveyed our collection of psychrophilic bacteria to determine the types of phosphatases they produce and whether any had heat-labile activities with potential applications. assays at different temperatures showed that the activity from one isolate was optimal at 45(deg)c and decreased dramatically above 55(deg)c. this isolate, d10, had the rod-coccus morphological cycle and cell wall amino acids associated with members of the arthrobacter genus. interestingly, we found that this strain made ...	1996	16535422
crystallization and preliminary x-ray analysis of a hydantoinase from arthrobacter aurescens dsm 3745.	l-hydantoinase from arthrobacter aurescens dsm 3745 has been purified to homogeneity and crystallized from polyethylene glycol solutions in a form suitable for x-ray diffraction analysis. the crystals have been grown by the sitting-drop variant of the vapour-diffusion method. x-ray diffraction studies show that the crystals belong to the monoclinic space group p2(1) with a = 111.2, b = 74.4, c = 146.5 a and beta = 106.7 degrees. its asymmetric unit contains four monomers related by 222 symmetry. ...	1996	15299588
purification, crystallization and preliminary x-ray crystal structure analysis of copper amine oxidase from arthrobacter globoformis.	beta-phenylethylamine oxidase from the gram-positive bacterium arthrobacter globoformis has been crystallized as three crystal forms. two belong to space group c2 and one to space group p2(1)2(1)2(1), respectively. the unit-cell volumes are consistent with one infunit of 70 644 da per asymmetric unit for the two monoclinic forms, and with two infunits per asymmetric unit for the orthorhombic crystals. three-dimensional intensity data have been recorded to 2.8a resolution for one of the monoclini ...	1996	15299744
meloidogyne incognita infested soil amended with chicken litter.	the effects of chicken litter on meloidogyne incognita in cotton, gossypium hirsutum cv. dpl50 were determined in field microplots. litters (manure and pine-shaving bedding) from a research facility and a commercial broiler house were used. treatments consisted of 0.25%, 0.5%, and 1% litter by dry weight of soil for each kind of litter. three control treatments consisted of soil not amended with litter, with and without nematodes, and one treatment to which mineral fertilizer was added at a nitr ...	1996	19277155
manganese(ii)-dependent extradiol-cleaving catechol dioxygenase from arthrobacter globiformis cm-2.	a manganese-dependent 3,4-dihydroxyphenylactate 2,3-dioxygenase from arthrobacter globiformis strain cm-2 (mndd) cloned in escherichia coli has been purified to homogeneity. sedimentation equilibrium analysis indicates an alpha 4 homotetrameric holoenzyme structure (4 x 38,861 da). steady-state kinetic analysis of mndd with a variety of substrates and inhibitors yields very similar relative rates to the known fe(ii)- and mn(ii)-dependent 3,4-dihydroxyphenylacetate 2,3-dioxygenases from pseudomon ...	1996	8555170
biodegradation of 4-chlorophenol via a hydroquinone pathway by arthrobacter ureafaciens cpr706.	a newly isolated arthrobacter ureafaciens, strain cpr706, could degrade 4-chlorophenol via a new pathway, in which the chloro-substituent was eliminated in the first step and hydroquinone was produced as a transient intermediate. strain cpr706 exhibited much higher substrate tolerance and degradation rate than other strains that degraded 4-chlorophenol by the hydroxylation at the second carbon position to form chlorocatechol. strain cpr706 could also degrade other para-substituted phenols (4-nit ...	1996	8931337
biodegradation of dimethylsilanediol in soils.	the biodegradation potential of [14c]dimethylsilanediol, the monomer unit of polydimethylsiloxane, in soils was investigated. dimethylsilanediol was found to be biodegraded in all of the tested soils, as monitored by the production of 14co2. when 2-propanol was added to the soil as a carbon source in addition to [14c]dimethylsilanediol, the production of 14co2 increased. a method for the selection of primary substrates that support cometabolic degradation of a target compound was developed. by t ...	1996	8953708
cloning and sequencing of a cluster of genes encoding novel enzymes of trehalose biosynthesis from thermophilic archaebacterium sulfolobus acidocaldarius.	trehalose biosynthesis genes, trez, trex and trey, encoding maltooligosyltrehalose trehalohydrolase (trez), glycogen debranching enzyme (trex), and maltooligosyltrehalose synthase (trey) have been cloned from the thermophilic archaebacterium sulfolobus acidocaldarius atcc33909. the amino-acid sequences deduced from trez, trex and trey are composed of 556, 713 and 720 amino-acid residues, respectively. trez and trey are 33-40% homologous to the corresponding enzymes from arthrobacter sp. q36. we ...	1996	8980629
flagellar proteins of motile spores of actinomycetes.	flagella of some of the actinoplanete genera were purified and the molecular sizes of their flagellin subunits compared by sds-page analysis to flagellins of cells of other bacteria. several species of actinoplanes have a major flagellar protein of subunit sizes of 42-43 kda and a lesser amount of a second protein, possibly a minor flagellin subunit, of 60 kda. the flagellar protein sizes of other actinoplanetes ranged from 32-43 kda (major) and 48-58 kda (minor). antibodies formed against the 4 ...	1996	8987497
biodegradability of oxidized poly(vinyl alcohol).	poly(vinyl alcohol) dehydrogenase (pvadh) purified from pseudomonas sp. 113p3 catalyzed an oxidation of poly(vinyl alcohol) (pva) in the presence of pyrroloquinoline quinone (pqq) to give a beta-diketone structure on pva. although pvadh oxidized not only enzymatically oxidized pva but also chemically oxidized pva, pva-degrading microorganisms, pseudomonas sp. 113p3 and arthrobacter tumescens sp. 52-1 grew on the enzymatically oxidized pva, but not on the chemically oxidized pva. this suggests th ...	1996	8987864
crystallographic and fluorescence studies of ligand binding to n-carbamoylsarcosine amidohydrolase from arthrobacter sp.	crystal structures of n-carbamoylsarcosine amidohydrolase (cshase; ec 3.5.1.59) have been analyzed by x-ray diffraction methods with two different inhibitors bound to the active site at 2.28 a and 2.37 a resolution. the catalytic center of the enzyme could be identified on the basis of these structures. the four substrate binding sites are situated at the intersubunit interfaces of the compact dimers ab and cd of the homotetrameric enzyme. both inhibitors inactivate the enzyme irreversibly throu ...	1996	8913306
nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from brevibacterium linens m18.	linocin m18 is an antilisterial bacteriocin produced by the red smear cheese bacterium brevibacterium linens m18. oligonucleotide probes based on the n-terminal amino acid sequence were used to locate its single copy gene, lin, on the chromosomal dna. the amino acid composition, n-terminal sequence, and molecular mass derived from the nucleotide sequence of an open reading frame of 798 nucleotides coding for 266 amino acids found on a 3-kb bamhi restriction fragment correspond closely to those o ...	1996	8919789
endophthalmitis involving an arthrobacter-like organism following intraocular lens implantation.		1996	8922827
hydroxylamine oxidation in heterotrophic nitrate-reducing soil bacteria and purification of a hydroxylamine-cytochrome c oxidoreductase from a pseudomonas species.	hydroxylamine oxidation was measured in four recently isolated heterotrophic nitrate-reducing bacteria belonging to the genera pseudomonas, moraxella, arthrobacter and aeromonas. a hydroxylamine-cytochrome c oxidoreductase activity was detected in periplasmic fractions of the pseudomonas and aeromonas spp. and in total soluble fractions of the arthrobacter sp. a monomeric 19-kda non-haem iron hydroxylamine-cytochrome c oxidoreductase was purified from the pseudomonas species and shown to be simi ...	1996	9082922
the species composition of oligotrophic bacterial communities of lake balaton--a numerical analysis.	from among the 565 aerobic and facultatively anaerobic, oligotrophic bacterial isolated obtained from the open water region of the balatonfüred-bay, 58 representative strains were selected for detailed taxonomic-physiological studies and numerical analyses as well as simultaneous comparisons with authentic bacterial strains isolated earlier by langó (1987) from different water habitat of the lake balaton. on the basis of 186 ceded differential diagnostic characteristics involved in these analyse ...	1996	9147724
quinaldine 4-oxidase from arthrobacter sp. rü61a, a versatile procaryotic molybdenum-containing hydroxylase active towards n-containing heterocyclic compounds and aromatic aldehydes.	quinaldine 4-oxidase from arthrobacter sp. rü61a, an inducible molybdenum-containing hydroxylase, was purified to homogeneity by an optimized five-step procedure. molecular oxygen is proposed as physiological electron acceptor. electrons are also transferred to artificial electron acceptors with e'o > -8 mv. the molybdo-iron/sulfur flavoprotein regiospecifically attacks its n-heterocyclic substrates: isoquinoline and phthalazine are hydroxylated adjacent to the n-heteroatom at cl, whereas quinal ...	1996	8617260
biosynthesis of topa quinone cofactor in bacterial amine oxidases. solvent origin of c-2 oxygen determined by raman spectroscopy.	resonance raman spectroscopy is an excellent technique for providing structural information on the 2,4, 5-trihydroxyphenylalanine quinone (tpq) cofactor in copper-containing amine oxidases. this technique has been used to investigate the copper- and o2-dependent biosynthesis of the tpq cofactor in phenylethylamine oxidase (peao) and histamine oxidase from arthrobacter globiformis. incubation of the holoenzyme in h218o causes frequency shifts at 1684(-26) cm-1 in peao and at 1679(-28) cm-1 in his ...	1996	8617737
cold shock and cold acclimation proteins in the psychrotrophic bacterium arthrobacter globiformis si55.	the psychrotrophic bacterium arthrobacter globiformis si55 was grown at 4 and 25 degrees c, and the cell protein contents were analyzed by two-dimensional electrophoresis. cells subjected to cold shocks of increasing magnitude were also analyzed. correspondence analysis of protein appearance distinguished four groups of physiological significance. group i contained cold shock proteins (csps) overexpressed only after a large temperature downshift. group ii contained csps with optimal expression a ...	1996	8655472