Publications

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naturally-occurring antibodies to bacillary teichoic acids. 19724622007
bovine mastitis due to bacillus cereus. 19724622049
[enterotropic properties of bacillus cereus toxin]. 19724622119
[bacillus cereus infection in a pullet]. 19724622182
the selective inhibition of microbial rna synthesis by salicylate. 19724622576
a toxic substance from aspergillus viridi-nutans. 19724622987
[food poisoning caused by s. aureus, cl. perfringens, b. cereus and salmonella species]. 19724623265
effects of sub-lethal heat-shock, -alanine, and l-alanine on germination and subsequent destruction of bacillus spores by pasteurization. 19724623376
characterization of autolysed membranes of bacillus cereus. characterization in membrane biosynthesis and binding of hemes to proteins of membrane subfraction l10p. 19724623433
[absorption of tetracycline in presence of bromelain after oral administration]. 19724623701
[fatal shock due to live culture of bacillus cereus]. 19724624046
buoyant density studies on natural and synthetic deoxyribonucleic acids in neutral and alkaline solutions. 19724624113
[sensitivity of bacillus cereus t to isoleucine, valine and leucine]. 19724624961
the production of penicillins in soils and seeds by penicillium chrysogenum and the role of penicillin -lactamase in the ecology of soil bacillus. 19724624991
the effect of local anesthetics on the hydrolysis of free and membrane-bound phospholipids catalyzed by various phospholipases. 19724625264
the lytic behavior of pure phospholipase a 2 and c towards osmotically swollen erythrocytes and resealed ghosts. 19724625506
antimicrobial properties of natural phenols and related compounds: obtusastyrene and dihydro-obtusastyrene.factors influencing the antimicrobial properties of obtusastyrene and dihydro-obtusastyrene were studied. both of these compounds were soluble in acetone, alcohol, and olive oil. in water, they were soluble at concentrations of 34 and 53 mug/ml, respectively. the minimal inhibitory concentrations against gram-positive bacteria and yeast were below 100 mug/ml. the compounds were not effective against gram-negative bacteria at 200 mug/ml or lower concentrations. with initial populations of cells g ...19724625626
the synthesis of phospholipase c by bacillus cereus and its relation to sporulation. 19724626007
phospholipase c from bacillus cereus and its use in studies of tissue thromboplastin. 19724626238
variability of dna content in individual cells of bacillus. 19724626246
appearance of muramic lactam during cortex synthesis in sporulating cultures of bacillus cereus and bacillus megaterium.evidence is presented for the appearance of muramic lactam during the late stages of sporulation at about the same time dipicolinic acid synthesis occurs.19724626506
multiple penicillin-binding components in bacillus subtilis, bacillus cereus, staphylococcus aureus, and escherichia coli. 19724626716
mutants of bacillus cereus strain t that produce thermoresistant spores lacking dipicolinate and have low levels of calcium. 19724627143
inhibition of fungal growth and sporulation by volatile metabolites from bacteria. 19724627147
regulation of dipicolinic acid biosynthesis in sporulating bacillus cereus. characterization of enzymic changes and analysis of mutants.some of the early enzymes in the lysine-biosynthetic pathway also function for dipicolinic acid synthesis in sporulating bacillus cereus t. 1. the first enzyme, aspartokinase, loses its sensitivity to feedback inhibition by lysing. this change occurs before the time of dipicolinic acid synthesis but at a time when diaminopimelic acid is required for spore cortex formation. 2. a possible regulatory change at a branch point in the pathway was studied by examining the properties of a key enzyme, di ...19724627586
the slow bactericidal effect of tetracycline and minocycline on wall-defective staphylococcus. 19724627649
location of calcium within bacillus spores by electron probe x-ray microanalysis.spectroscopic microanalysis of the element-characteristic x rays produced by a scanning electron microprobe was employed to detect calcium and carbon in both intact and thin-sectioned spores of bacillus cereus t and b. megaterium qm b1551. linear scan profiles and multilinear scan images of the x-ray emissions for calcium (ca(kalpha)) were compared with those for carbon (c(kalpha)) as an index of mass. location was accomplished by stereological comparisons with secondary electron images and conv ...19724627927
bacillus cereus-induced fluid accumulation in rabbit ileal loops.the usefulness of the ligated rabbit ileal loop as an experimental model of bacillus cereus food poisoning was investigated. positive responses, as measured by fluid accumulation in the loop, were obtained from 19 of 22 strains of b. cereus. four of six strains of b. thuringiensis also elicited fluid accumulation, but eight strains of other bacillus spp. failed to evoke a response. the growth medium employed markedly affected the ability of a given strain of b. cereus to provoke a response. brai ...19724627964
effect of ultrasonic waves on the heat resistance of bacillus cereus and bacillus licheniformis spores.heat resistance of bacillus cereus and bacillus licheniformis spores in quarter-strength ringer solution decreases markedly after ultrasonic treatments which are unable to kill a significant proportion of the spore population. this effect does not seem to be caused by a loss of ca(2+) or dipicolinic acid. the use of ultrasonics to eliminate vegetative cells or to break aggregates in bacillus spore suspensions to be used subsequently in heat resistance experiments appears to be unadvisable.19724627969
iodination of penicillinase isoenzymes. 19724628487
lipid composition, permeability and structure of biological membranes. 19724628621
[effect of different nitrogen and carbon sources on glutamic acid and valine accumulation in culture liquid filtrates from bacterium agile 745 and bacillus cereus 1089 isolated from typical irrigated serozem]. 19724628762
ovine perinatal mortality associated with bacillus cereus. 19724628926
[isolation and physicochemical properties of berromycin, a new antineoplastic antibiotic]. 19724628944
[preparation of lyophilized inocula for microbiological determination of antibiotics]. 19724629094
immunological distinction of mycobacterial beta-lactamases.beta-lactamase-containing fractions have been isolated from myobacterium tuberculosis strain r(1)r(v) and m. smegmatis strains nctc 8158 and t-64. antisera raised in rabbits to these fractions demonstrated no cross-reactivity with a commercial bacillus cereus beta-lactamase. antiserum to either strain of m. smegmatis revealed no cross-reactivity with m. tuberculosis r(1)r(v), one specificity in common with the other m. smegmatis strain, and two specificities unique to the immunizing fractions. f ...19724629125
synthesis of inducible phospholipase a with antibacterial activity by bacillus cereus. 19724629230
abortion in cattle associated with bacillus cereus. 19724629648
pathogenicity of bacillus cereus for pregnant ewes and heifers. 19724629649
[the influence of escherichia coli and staphylococcus on the multiplication and toxigenicity of bacillus cereus in several food products]. 19724633468
[monovalent repression caused by isoleucine, valine or leucine of the alpha-acetohydroxyacid synthetase of bacillus cereus t]. 19724631973
isolation and some properties of glyceraldehyde 3-phosphate dehydrogenase from vegetative cells of bacillus cereus. 19734632199
the use of -lactamases in the clinical laboratory. 19734632253
photoprotection by dipicolinate against inactivation of bacterial spores with ultraviolet light.the resistance of three types of bacillus cereus t spores to ultraviolet radiation corresponded to their dipicolinic acid (dpa) content. photoprotection against ultraviolet light was observed in dpa-containing spores and in dpa-less spores irradiated through calcium dipicolinate.19734632312
occurrence of glucosamine residues with free amino groups in cell wall peptidoglycan from bacilli as a factor responsible for resistance to lysozyme.analysis by dinitrophenylation techniques revealed the occurrence of significant amounts of glucosamine residues with free amino groups in the peptidoglycan component of cell walls isolated from bacillus cereus, bacillus subtilis, and bacillus megaterium. a close correlation was demonstrated between the content of n-unacetylated glucosamine residues in the peptidoglycan component and the resistance of the cell walls to lysozyme. these lysozyme-resistant cell walls and peptidoglycan were converte ...19734632317
role of glutathione in the morphogenesis of the bacterial spore coat.there is a marked increase in the half-cystine content of bacterial spores, especially the coat layers at the time of formation of the outer coat. when a cysteine auxotroph of bacillus cereus t is grown on limiting cysteine, the spores contain the normal content of half-cystine, suggesting an alternate source. glutathione appears to be such a supply of cysteine since it is hydrolyzed during sporulation and there are increased activities of the hydrolyzing enzymes at the same time. in addition, a ...19734632393
use of fluorouracil-uracil combinations to study growth accompanied by insufficient deoxyribonucleic acid synthesis in bacillus cereus.5-fluorouracil (fu) at a concentration of 16 mum almost totally inhibited deoxyribonucleic acid (dna) synthesis and cell division by bacillus cereus, whereas growth continued at an exponential rate (25% of control for at least 3 h). in cultures simultaneously given 160 mum uracil (u) along with the fu, dna synthesis still stopped, but cell division continued for one generation at the control rate and at a much slower rate beyond that; in the meantime, cell mass continued to increase at an essent ...19734632399
ascaridia galli (nematoda): development and survival in gnotobiotic chickens. 19734632462
effect of dipicolinic acid on the ultraviolet radiation resistance of bacillus cereus spores. 19734632644
the bond hydrolyzed by cardiolipin-specific phospholipase d. 19734632675
oxidative activation of bacillus cereus spores.a study was made of the activation of bacillus cereus strain t spores by using the oxidizing agent sodium perborate. the degree of activation was measured with constant germination conditions by using l-alanine, inosine, adenosine, and l-alanine plus adenosine as germination stimulants. the germinal response following the various treatments was compared with the responses obtained with heat activation. it was concluded that the optimal time for activation with 30 mm sodium perborate at room temp ...19734632848
synthesis and antimicrobial properties of 17 -isopentyloxy-4-aza-5 -androstane and the 4-methyl derivative. 19734633451
[the biology and taxonomy of entomopathogenic sporiferous bacteria]. 19734633762
thromboembolic sequelae to indwelling silastic cannulas in sheep arteries. 19734634084
isolation and purification of antibiotic material from physarum gyrosum.the myxomycete physarum gyrosum was cultured in its plasmodial stage on agar plates containing 0.025 m phosphate buffer at ph 6.5, 2% bakers' yeast, and 0.2% glucose and was supplemented with live escherichia coli. extracts of these plasmodia contained several antibiotic substances. antibiotic materials were partially purified by dialysis of the agar medium-mold mixture, evaporation of the dialyzate, and butanol extraction of the residue. further purification in two paper and two thin-layer chro ...19734799591
pericillinase inhibition. 19734629666
development of a quantitative immunological assay for the study of spore coat synthesis and morphogenesis.a quantitative assay employing (125)i-labeled antibody has been developed for bacillus cereus t spore coat protein. populations of antibody molecules with various affinities for inner or outer coat can be prepared by selective adsorption to and elution from different coat preparations. adsorption to and elution from intact spores results in an antibody preparation at least 15 times more reactive to outer coat. this antibody is useful for measuring the time and extent of spore coat maturation, i. ...19734631708
kinetics of ribonucleic acid-deoxyribonucleic acid membrane filter hybridization. 19734631862
characterization of bacteria by ultraviolet spectroscopy. 19734631878
action of lysozyme on oligosaccharides from peptidoglycan n-unacetylated at glucosamine residues. 19734631898
serum oxytetracycline concentrations after intravenous and intramuscular administration in horses. 19734630538
preparation of pure choline and ethanolamine plasmalogens with the use of purified lipase from porcine pancreas. 19734373362
[ozone for disinfection of water contaminated with vegetative and spore forms of bacteria, fungi, and viruses]. 19734200343
destruction of psychrophilic strains of bacillus by chlorine. 19734200453
development of ultraviolet resistance in sporulating bacillus cereus t.ultraviolet (uv) resistance patterns of sporulating bacillus cereus were determined. six different categories of uv resistance were discernible as the organism progressed from exponential growth to the free, mature spore. light microscope observations allowed the assignment of certain sporulation stages to specific uv resistance categories. marked changes in uv resistance were associated with stages iii, mid-iv, and post-iv of sporulation. dipicolinic acid was shown to sensitize forespores to uv ...19734200858
carbon monoxide production from hydroxocobalamin by bacteria. 19734200896
advances in the treatment of infectious diseases. 19734201148
measurement of heat resistance parameters for spores isolated from canned products. 19734201180
sensitivity of three selected bacterial species to ozone.the minimal lethal concentration of ozone in water was determined for three bacterial species: escherichia coli, bacillus cereus, and bacillus megaterium. a contact period of 5 min was selected. the lethal threshold concentration for the cells of b. cereus was 0.12 mg/liter while that for e. coli and b. megaterium was 0.19 mg/liter. low concentrations of ozone were ineffective when organic matter was present to interfere with the action of ozone on the bacterial cells. also determined during the ...19734201643
germination of spores of bacillus cereus in milk and milk dialysates: effect of heat treatment. 19734201828
screening and isolation of penicillinase inhibitor, ka-107.it is known that penicillin resistance of bacteria is mainly caused by the inactivation of penicillin by penicillinase derived from such strains. we have developed a screening procedure for penicillinase inhibitors. several microorganisms were found to produce such inhibitors, and from the culture filtrate of streptomyces gedanensis atcc 4880 a penicillinase inhibitor, named ka-107, was isolated. the characteristics of this inhibitor were revealed by an in vitro test by using penicillinase deriv ...19734202340
in vitro stimulation of lecithin synthesis in rat liver mitochondria and microsomes after treatment with phospholipase c. 19734202546
differences in susceptibility to phospholipase c of free and membrane-bound phospholipids of mycoplasma hominis. 19734202552
analytical determination of the buoyant density of dna in acrylamide gels after preparative cscl gradient centrifugation. 19734202826
kinetics of beta-lactamase inactivation of penicillins. ii. effect of competitive inhibitors. 19734203115
scanning electron microscopy of bacterial colonies.a technique is described for observing bacterial colony growth. bacillus cereus, b. subtilis, and b. cereus var. mycoides were grown on strips of dialysis membrane layered on nutrient agar. microcolonies of the organisms on strips were fixed in formalin vapor in situ; the strips then were removed from the agar and secured to scanning microscope specimen stubs without markedly disturbing the cellular arrangement. scanning electron micrographs clearly depict morphology of individual cells, as well ...19734203336
[effect of water activity on the development of microorganisms in fine-pored systems (kaolinite)]. 19734203670
thin layer chromatography methods for detecting hippurate hydrolase activity among various bacteria (pseudomonas, bacillus, enterobacteriaceae). 19734203919
comparative immunological and electrophoretic studies on ribosomal proteins of bacillaceae. 19734203929
comparison of ribosomal rna-binding protein "l2" isolated from different bacterial species. 19734203930
toxicity of metabolites produced by the "alternaria". 19734198474
tetracycline interference with the bactericidal effect of serum. 19734198604
purification and properties of an extracellular polysaccharide containing amino sugars formed by bacillus cereus. 19734198886
morphological abnormalities of fungi induced by volatile microbial metabolites. 19734199221
release and recovery of forespores from bacillus cereus.a method is described which makes possible the release of immature forespores from sporulating cells at specific stages of development, from the completion of stage iii through to mature spore formation. with the aid of zonal density gradient centrifugation, the method makes possible the recovery of quantities of forespores ample for biochemical and physical studies. with the capability to examine forespores and some mother cell components independently, we have established that several enzymes ...19734199507
exosporium and spore coat formation in bacillus cereus t.the exosporium of bacillus cereus t was first observed as a small lamella in the cytoplasm in proximity to the outer forespore membrane (ofsm) near the middle of the sporangium. serial sections, various staining methods, and enzyme treatments failed to show any connections between the small lamella and the ofsm. the advancing edge of the exosporium moved toward the polar end of the cell until the spore was completely enveloped. the middle coat was formed between the exosporium and the ofsm from ...19734199508
induction of biohydrogenation of oleic acid in bacillus cereus by increase in temperature. 19734197961
extracellular factor synthesized by bacillus cereus which evokes a dermal reaction in guinea pigs.a guinea pig skin assay was developed as a measure of ileal loop fluid-inducing factor in the rabbit and possibly of the causative principle of bacillus cereus food poisoning. a green or bloody necrotic reaction was produced in the guinea pig skin by injection of culture filtrates from 21 of 24 b. cereus strains tested, and by no other bacillus species tested except for the closely related b. thuringiensis. the skin factor was synthesized and excreted by logarithmically growing cells, inactivate ...19734198101
environmental factors internal to the host that affect the sucess of microbial insecticides. 19734198128
electron microscopy of bacillus subtilis protoplast membrane after treatment with phospholipase a 2 and phospholipase c. 19734198136
polysaccharides of the genus bacillus cross-reactive with the capsular polysaccharides of diplococcus pneumoniae type 3, haemophilus influenzae type b, and neisseria meningitidis group a.we studied 174 strains of the genus bacillus for cross-reacting antigens to the capsular polysaccharides of groups a and c meningococcus, types i and iii pneumococcus, and haemophilus influenzae type b. cross-reactions were detected by immunodiffusion in agarose gel by using type-specific antisera and confirmed by absorption and inhibition experiments. of 20 bacillus pumilis strains, six had an antigen cross-reacting with group a meningococcal polysaccharide. other cross-reactions included one s ...19734150383
identification of small amounts of antibiotics by electrophoresis and bio-autography. 19734134372
influence of cell-wall thickness on cell division: electron microscopic study with bacillus cereus. 19734121312
ultrastructural study of poly- -hydroxybutyrate granules from bacillus cereus.the freeze-etching technique was used to examine the effects of fracturing and etching on the appearance of poly-beta-hydroxybutyrate granules from bacillus cereus. these granules were examined in extracts isolated by hypochlorite or by sonic treatment, and also in fixed and unfixed intact cells; in the latter case they were compared with granules in thin sections of intact cells. after freeze-fracturing, the diameter of the granules in intact cells was between 240 and 720 nm. the granules consi ...19734123236
electron transport system associated with membranes of bacillus cereus during vegetative growth and sporulation.membranes isolated from bacillus cereus atcc 4342 during vegetative growth and during sporulation contained cytochromes b, c and a + a(3) as well as flavoprotein as determined from reduced-minus-oxidized difference spectra. although there appeared to be no qualitative change in the cytochromes, there was a significant increase in the amount of cytochromes associated with membranes isolated from sporulating cells. succinate and nicotinamide adenine dinucleotide (reduced form) (nadh) reduced the s ...19734125246
production of bacteriophage by temperature-sensitive sporulation mutants of bacillus cereus t.five temperature-sensitive sporulation mutants of bacillus cereus t have been isolated. these mutants are blocked at stage 0 of sporulation at the restrictive temperature (37 c) but are able to sporulate at nearly normal frequencies at the permissive temperature (26 c). a bacteriophage that forms a stable lysogen in the parent strain is induced at increased frequencies in the mutants. this induction is accompanied, in some of the mutants, by a reduction in immunity to the phage. revertants, sele ...19734126824
a dissection of the inhibitory effects of 5-fluorouracil in bacillus cereus. 19734127017
kinetics of -lactamase inactivation of penicillins i: effect of side-chain structure, ionic strength, ph, and temperature. 19734196205
rna polymerase from vegetative cells and spores of bacillus cereus t. ii. peptide composition. 19734196221
penicillin-binding component of bacillus cereus.(14)c-penicillin is irreversibly bound by bacillus cereus 569. incubation of penicillin-treated cells in a cell wall digestion medium results in solubilization of approximately 60% of the irreversibly bound lable. the extent of the solubilization is the same when cells are prepared by either a cold or 37 c treatment procedure. however, spheroplasts prepared by the cold treatment are leaky. when the resulting spheroplasts are incubated in supplemented medium, reduced rates and levels of penicilli ...19734196243
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