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high-level chromosomal gentamicin resistance in streptococcus agalactiae (group b).this is the first report of high-level gentamicin resistance in a group b streptococcus. strain b128 of serotype ii was isolated from an infected leg wound in 1987. b128 was resistant to high levels of gentamicin as well as of all other available aminoglycosides and was also resistant to tetracyclines. no bactericidal synergism was found between ampicillin or vancomycin and any of these aminoglycosides. gentamicin, kanamycin, streptomycin, and tetracycline resistance determinants transferred by ...19902203311
intra- and inter-specific transformation of streptococcus pneumoniae to penicillin resistance.transformation studies were carried out with a penicillin susceptible (mic 0.006 mg/l) laboratory strain of streptococcus pneumoniae as recipient. donor dna was prepared from two clinical isolates of s. pneumoniae, four isolates of s. mitior and five isolates of s. sanguis. dna from both isolates of s. pneumoniae generated penicillin-resistant transformants (mics 0.03-2.0 mg/l). in addition, one isolate each of s. mitior and s. sanguis transformed the recipient to increased penicillin resistance ...19902211443
incidence of bacteriocinogeny among fresh isolates of streptococcus mutans.among 139 streptococcus mutans fresh isolates tested, using the deferred-antagonism test and streptococcus sanguis ny 101 as the indicator strain, we observed that the frequency of detection of inhibition zones was reduced by 19% (from 53 to 34%) when arginine (1%) was used in the overlay agar. among pigmented strains, the frequency of mutacin-like production was 70%. the frequency with which inhibition zones were detected varied from 7 to 91%, depending on the indicator strain used. these resul ...19902224647
binding of viridans group streptococci to human platelets: a quantitative analysis.the binding of viridans group streptococci with human platelets was analyzed by two-color flow cytometry. binding was detected within 15 s of mixing bacteria and platelets. at ratios of bacteria to platelets of 1:1, 10:1, 100:1, and 1,000:1, the percentages of bound streptococci (mean +/- standard deviation) were 93.2% +/- 5.4%, 80.0% +/- 8.6%, 39.8% +/- 11.1%, and 12.5% +/- 2.0%, respectively. binding of labeled bacteria was reversed by adding a 500-fold excess of unlabeled streptococci. these ...19902228249
the effect of sucrose application and implantation of mutans streptococci on the microbial composition of three-week experimental plaque--an in situ study.this study describes the predominant cultivable microflora of three-week-old plaque samples obtained from human enamel sites, on the basis of microbial identification of over 9000 fresh isolates. lower removable appliances, on which were mounted enamel sections and slabs, were worn by five young adult subjects under three experimental protocols. these were (1) 'normal' plaque conditions, (2) extra-oral sucrose applications nine times daily, and (3) inoculation of each subject's own mutans strept ...19902229607
streptococcal endocarditis temporally related to transesophageal echocardiography.a case of streptococcal sanguis endocarditis temporally related to a transesophageal echocardiogram is reported; and the literature on the incidence of bacteremia occurring during transesophageal echocardiography is reviewed. on the basis of this case and review of the literature, a reevaluation of the current guidelines for endocarditis prophylaxis during this procedure is recommended.19902245038
arginine catabolism by strains of oral streptococci.arginine catabolism via the arginine deiminase pathway was found in streptococcus sanguis 903. citrulline and ornithine were released from resting cells incubated with arginine, arginine-containing peptides, or saliva. maximum arginine catabolism by resting cells of s. sanguis 903 was found in the ph range 7-8 and at 45-48 degrees c. arginine deiminase activity was found in the cytoplasm and in the cell-wall extract of this strain, while ornithine carbamoyltransferase activity was found in the c ...19902248769
lingual cellulitis causing upper airway obstruction.a case is reported in which upper airway obstruction occurred in an immunosuppressed patient due to the presence of lingual cellulitis.19902248938
purification and characterization of an outer membrane protein adhesin from haemophilus parainfluenzae hp-28.outer membranes were isolated from haemophilus parainfluenzae hp-28 by a mild extraction method followed by sephadex g-150 gel filtration chromatography. the first peak (pool 1) recovered contained an activity which inhibited adherence of hp-28 cells to saliva-coated spheroidal hydroxyapatite. sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) of pool 1 revealed a dominant protein band of 34 kda. the sds-page-purified 34-kda protein was excised from the gel and used for antibod ...19902254013
platelet-interactive products of streptococcus sanguis protoplasts.to isolate a more native, platelet-interactive macromolecule (class ii antigen) of streptococcus sanguis, cultured protoplasts were used as a source. protoplasts were optimally prepared from fresh washed cells by digestion with 80 u of mutanolysin per ml for 75 min at 37 degrees c while osmotically stabilized in 26% (wt/vol) raffinose. osmotically stabilized forms were surrounded by a 9-nm bilaminar membrane, as shown by transmission electron microscopy. protoplasts were cultured in chemically d ...19902254032
predominance of resistant oral streptococci in saliva and the effect of a single course of josamycin or erythromycin.we have investigated the influence of the macrolides erythromycin and josamycin on the selection of resistant oral streptococci by sampling saliva of volunteers before and after oral administration of 1.5 g of either agent followed by a further 0.5 g of the same drug after 6 h, as in routine prophylaxis for oral or dental procedures. the small proportion of resistant organisms present before antibiotic administration increased substantially 48 h after the macrolides were given. after antibiotic ...19902254223
ability to bind salivary alpha-amylase discriminates certain viridans group streptococcal species.a collection of 144 viridans group streptococcal strains recently characterized as part of a taxonomic study was examined for the ability to bind salivary alpha-amylase. this property was found in most strains of streptococcus gordonii and streptococcus mitis and in occasional strains of streptococcus anginosus and streptococcus salivarius. in contrast, all strains of streptococcus sanguis, streptococcus oralis, streptococcus vestibularis, and streptococcus mutans lacked alpha-amylase-binding ca ...19902254435
comparison of the initial streptococcal microflora on dental enamel in caries-active and in caries-inactive individuals.this study compared the initial (4 h) microflora on enamel in 7 caries-active and in 7 caries-inactive adolescents. in both groups the microflora was dominated by streptococci which comprised 61 and 78% (median values) of the total viable counts in caries-active and caries-inactive individuals, respectively (p less than 0.01). identification of a total of 700 streptococcal isolates according to a recently revised classification showed that the predominant streptococci belonged to the species str ...19902276164
platelet aggregation by oral streptococci.one proposed mechanism in the pathogenesis of infective endocarditis is the direct aggregation of platelets by the bacteria causing the disease. some, but not all, strains of streptococcus sanguis have been reported to aggregate platelets but the taxonomy of this and related taxa has changed recently. the ability to aggregate platelets by 24 genetically grouped laboratory stock strains was studied along with 8 recent isolates from cases of endocarditis. strains belonging to s. sanguis could aggr ...19902283042
method and parameters for genetic transformation of streptococcus sanguis challis.a simple procedure for genetic transformation of streptococcus sanguis challis was developed and standardized. during the exponential phase of growth, cells became competent while growing as diplococci in broth containing 10% foetal calf serum. high levels of competence were maintained by the cultures for 60 min. competent cells could be stored frozen without loss of competence for at least three years. using total chromosomal dna as donor, the dose-response curve for transformation of a point m ...19902284501
antistaphylococcal activity of pentamidine.pentamidine isethionate was bacteriostatic against staphylococcus aureus, staphylococcus epidermidis, streptococcus pyogenes, streptococcus sanguis, micrococcus sp., and candida albicans. s. aureus was inhibited by concentrations of 16 to 64 micrograms/ml and killed by 64 to greater than or equal to 128 micrograms/ml. staphylococcal killing was consistently greater in the presence of cations and was unaffected by methicillin resistance.19902285292
cellular coaggregation of oral streptococcus milleri with actinomyces.oral isolates of streptococcus milleri were examined for their ability to coaggregate with actinomyces. of the 68 s. milleri strains tested, including 3 reference strains, 40 strains coaggregated with actinomyces naeslundii wvu45 (actinomyces coaggregation group b) and 36 strains coaggregated with actinomyces viscosus t14v (actinomyces coaggregation group a). all s. milleri strains of serotypes b (4 strains), e (2 strains), and f (24 strains) coaggregated with both of the actinomyces. the coaggr ...19902294047
colonization resistance of defined bacterial plaques to streptococcus mutans implantation on teeth in a model mouth.we investigated the ability of streptococcus mutans c67-1 to colonize simple bacterial plaques and the effects of age and stability of the pre-formed plaque on colonization resistance. mixed-plaques of actinomyces viscosus wvu627, 'streptococcus mitior' lpa-1, and veillonella dispar omz193 were grown on tooth segments, mounted back to back for simulation of approximal sites in a model mouth for 66 h. s. mutans c67-1 was either included in the original inoculum or super-inoculated onto the develo ...19902307751
the echocardiographic recognition of an atrial myxoma vegetation. 19902309613
isolation of acid producing sorbitol-adapted bacteria from dental plaque using selective agar media.acidogenic bacteria metabolizing sorbitol were isolated and identified from dental plaque of twenty nine volunteers, claiming to be users (sixteen) and non-users (thirteen) of sorbitol-containing chewing gum and candy. sixty bacterial strains were isolated, using improved selective agar media. the taxonomy of twenty strains, all gram-positive cocci, was determined employing a customized bbl minitekr system and other biochemical reactions. the majority of these strains represented streptococcus s ...19902314301
[infectious endocarditis following orthotopic heart transplantation].infection remains a major problem in the early phase after heart transplantation. immunosuppressive therapy is the most important predisposing factor. it may also reactivate preexisting latent endogenous infections. unspecific symptoms and a chronic clinical course, as described in this report, may suggest infective endocarditis of the cardiac allograft. from this case, we do not suggest a general antibiotic prophylaxis for heart transplant recipients; however, special precaution should be consi ...19902316278
enterococcal resistance to vancomycin and related cyclic glycopeptide antibiotics.enterococci belonging to various species resistant to vancomycin and related cyclic glycopeptide antibiotics have been isolated from hospitalized patients in france, the uk and the usa. all such strains examined display inducible synthesis of a membrane protein associated with resistance. the mechanism by which the membrane protein acts has not been definitively established, but it may block the access of the antibiotic to its peptidoglycan target. that the protein could be a bypass enzyme has n ...19902318215
differential response to benzylpenicillin in vivo of tolerant and non-tolerant variants of streptococcus sanguis ii.a variant that was highly tolerant to benzylpenicillin was obtained from a non-tolerant clinical isolate of streptococcus sanguis ii by repeated exposure to penicillin. the rabbit model of endocarditis was used to investigate the efficacy of a high dose regimen of benzylpenicillin (250 mg/kg; peak serum concentration c. 25 mg/l) in the prophylaxis and treatment of endocarditis during challenge or infection with the non-tolerant parent strain or its tolerant variant. the two strains exhibited a s ...19902318747
amylase-binding as a discriminator among oral streptococci.the ability of 51 strains, belonging to streptococcus sanguis, 's. mitior', s. oralis and related groups, to bind salivary amylase was studied. most strains were grouped according to their dna-relatedness and then compared using 14 phenotypic tests. s. mitis, 's. mitior' and three relatively new groups of strains ('cr', 'mgh' and 'tufted mitior') bound salivary amylase, while strains of s. sanguis and s. oralis did not. the ability of strains to bind amylase or not was remarkably consistent with ...19902323538
vancomycin resistance is encoded on a pheromone response plasmid in enterococcus faecium 228.in enterococcus faecium 228, vancomycin resistance is encoded on a 55-kilobase conjugative plasmid, phkk100. this plasmid was transferred with high frequency into susceptible strains of enterococcus faecalis and conferred responses to pheromones produced by e. faecalis and streptococcus sanguis. phkk100 is the first plasmid described that mediates both vancomycin resistance and pheromone response.19902327781
cohesion between oral streptococci and neisseria pharyngis on saliva-coated glass, in the presence and absence of sucrose.ten strains of oral streptococci, comprising nine species, were screened for their in vitro cohesive interactions with a freshly isolated strain of neisseria pharyngis. strains of mutans streptococci, streptococcus salivarius, streptococcus sanguis i, streptococcus milleri and streptococcus mitis were able to cohere with n. pharyngis cells attached to a salivary pellicle adsorbed to glass. the cohesion was either due to coaggregation between the different genera or modification of the salivary p ...19902329945
adherence of mutans streptococci to other oral bacteria.adherence of mutans streptococci to strains of actinomyces viscosus, streptococcus sanguis, and streptococcus mitis immobilized on a nitrocellulose membrane was measured. strains of streptococcus mutans, s. sobrinus, and s. rattus bound in a lactose-independent manner to a variety of the actinomyces and streptococci. most of these reactions could proceed in the presence of whole saliva although adherence of s. rattus bht to the streptococci was inhibited by salivary molecules. in contrast, adher ...19902341174
involvement of human mucous saliva and salivary mucins in the aggregation of the oral bacteria streptococcus sanguis, streptococcus oralis, and streptococcus rattus.the contribution of human parotid (par) and submandibular/sublingual (sm/sl) saliva and of the human whole salivary mucin fraction (hwsm) to saliva-induced bacterial aggregation was studied for s. sanguis c476, s. oralis i581, and s. rattus hg 59. the mucous sm/sl saliva showed a much higher aggregation potency towards the s. sanguis and s. oralis strain than did the serous par saliva. the sm/sl saliva-induced aggregation was observed after 30 min, at 60 min followed by the par saliva-induced ag ...19902353808
biochemical mechanisms of enhanced inhibition of fluoride on the anaerobic sugar metabolism by streptococcus sanguis.the effect of fluoride on acid production by streptococcus sanguis atcc 10556 was compared under anaerobic and aerobic conditions. the rate of acid production under constant-ph and ph-free-fall conditions was determined during glucose metabolism by resting cells. anaerobic glycolysis was inhibited more strongly by fluoride than was aerobic glycolysis. intracellular levels of 3-phosphoglyceric, 2-phosphoglyceric, and phosphoenolpyruvic acids were lower under anaerobic conditions than under aerobi ...19902355117
atypical supraglottitis caused by streptococcus sanguis.a case of atypical supraglottitis in an 11-year-old boy is presented. the epiglottis was only mildly inflamed. the organism isolated from blood culture was streptococcus sanguis. this organism is a normal commensal of the oral cavity and has not previously been reported as a cause of supraglottitis.19902370475
characterization of streptococcus gordonii (s. sanguis) pk488 adhesin-mediated coaggregation with actinomyces naeslundii pk606.intergeneric coaggregation of streptococcus gordonii (s. sanguis) pk488 and actinomyces naeslundii pk606 was studied by using coaggregation-defective (cog-) mutants of both strains. a streptococcal protein of 38 kilodaltons was identified with anti-s. gordonii serum absorbed with cog- cells of the streptococcus. absorbed immunoglobulin g specifically blocked coaggregation of the streptococcus-actinomyces pair but did not affect the coaggregation of the streptococcus with other coaggregation part ...19902387635
purification and partial characterization of a 65-kda platelet aggregation-associated protein antigen from the surface of streptococcus sanguis.cells of streptococcus sanguis express a collagen-like immunodeterminant (class ii antigen) on their cell walls that induces aggregation of platelets in plasma. these platelet aggregation-associated proteins (paaps) are recovered in cell-free preparations obtained from cells of s. sanguis after 5 min of sonic or limited trypsin treatment. pretreatment of platelet-rich plasma with these soluble preparations selectively inhibits platelet aggregation in response to s. sanguis cells. a paap antigen ...19902387841
structure of a streptococcal adhesin carbohydrate receptor.interactions between complementary protein and carbohydrate structures on different genera of human oral bacteria have been implicated in the formation of dental plaque. the carbohydrate receptor on streptococcus sanguis h1 (one of the primary colonizing species) that is specific for the adhesin on capnocytophaga ochracea atcc 33596 (a secondary colonizer) has been isolated from the streptococcal cell wall, purified, and structurally characterized. the hexasaccharide repeating unit of the polysa ...19902387843
cerebral botryomycosis: case study.after oral surgery, a 32-year-old man developed a brain abscess. actinomycosis was suspected due to history, clinical findings, response to penicillin therapy, and demonstration of "sulfur granules" in the surgical specimen, but anaerobic cultures were negative for actinomyces. aerobic cultures yielded streptococcus sanguis and pseudomonas cepacia. coccoid organisms demonstrated histologically reacted positively with periodic acid-schiff, gomori's methenamine silver, and brown and brenn stains, ...19902388002
stimulation of the growth of streptococcus sanguis (nctc 7864) by zinc in vitro.zinc, which is present in many materials used in dentistry, including restorative and endodontic materials and mouthwashes, is reputedly bactericidal. however, the addition of this element at concentrations in the range 10-50 ppm to cultures of streptococcus sanguis (nctc 7864) stimulated the growth of the micro-organism relative to that of controls. a second antibacterial element, namely fluoride, reduced growth. similar results were obtained whether the organism was grown aerobically or anaero ...19902391185
[immunological analysis of streptococcus sanguis antigens].the exact cause of behçet's disease is still unknown. recently, advanced bacterial studies revealed that behçet's disease was related to streptococcus (s.) sanguis. however, the antigenicity of s. sanguis has not been fully studied. in this study, we clarified the antigenicity of s. sanguis. we made s. sanguis antigens: mechanical braun extract (mbe) and crude cell wall (ccw) from the whole cells, membrane fraction (mf) and cytoplasmic fraction (cf) from mbe, cell wall fragment (cwf) and lipotei ...19902396588
[a case report of mitral valve regurgitation due to the infective endocarditis associated with the straight back syndrome].a 54 year-old man was admitted to our hospital with a compliant of high fever following the dental treatment. he had the straight thoracic spine with absence of physiological kyphosis. blood culture was positive for streptococcus sanguis i. echocardiography revealed the mitral valve regurgitation with some vegetation at the ruptured chordae tendinae of the posterior leaflet. the operation was performed after the sign of the inflammation was disappeared. we performed the mitral valve reconstructi ...19902398884
an investigation into the mechanism of protection by local passive immunization with monoclonal antibodies against streptococcus mutans.local oral passive immunization with streptococcus mutans-specific monoclonal antibody (mab) (guy's 13) prevented recolonization by indigenous s. mutans in human volunteers who had first been treated with a conventional antibacterial agent (chlorhexidine). the f(ab')2 fragment of the mab was as protective as the intact immunoglobulin g, but the fab fragment of the molecule failed to prevent recolonization of s. mutans. in subjects receiving the mab fab fragment, s. mutans levels in dental plaque ...19902401569
glucanase-producing organisms in human dental plaques.selective media were used to isolate a wide range of bacteria from sixty human dental plaques. glucanase activities of the isolates were determined on dextran- and starch-containing media. all sixty samples of dental plaque yielded some colonies showing amylolytic and dextranolytic activities. the glucanase-producing organisms comprised 20% of the isolates. of these 38% were gram-positive rods, 27% gram-positive cocci, 28% gram-negative rods and 7% were gram-negative cocci. the cultural groups m ...19901691432
influenza a virus potentiates bacteria-induced histamine release. examination of normal individuals and patients allergic to bacteria.influenza a virus was found to enhance basophil histamine release induced by escherichia coli, salmonella enteritidis, staphylococcus aureus, streptococcus pneumoniae and streptococcus sanguis, but did not per se release histamine. this potentiating effect of the virus was seen both when the bacteria-induced mediator release was ige-dependent (i.e. patient allergic to bacterium) and when the bacterium caused histamine release by a non-immunological mechanism independent of ige (putative sugar-le ...19901700889
identification of mutans streptococci with monoclonal antibodies.mutans streptococci have been correlated with dental caries. the identification of the species within this group is still a problem. the characterization of a monoclonal antibody (mab) omvu10 against s. sobrinus as well as the isolation and characterization of mabs against s. mutans (omvu30 and omvu31), s. cricetus (omvu40) and mutans streptococci (omvu2) is described. the epitope specificity for omvu10 and omvu31 was cell-wall antigen b in both cases although both mabs recognized different spec ...19901707251
comparison of streptococcus mutans and streptococcus sanguis receptors for human salivary agglutinin.oral streptococci vary in their susceptibility to salivary agglutinin-mediated aggregation. to understand the molecular basis of this specificity, the structure and function of receptors for agglutinin from streptococcus mutans kpsk2 (msl-1) and streptococcus sanguis m5 (ssp-5) were compared. immunological screening of an s. mutans kpsk2 genomic dna library yielded two identical clones expressing a streptococcal protein that co-migrated with a 220 kda peptide in sds extracts from this organism. ...19901708078
[ecosystem of microbial flora in periodontal pockets, in vitro].this study was designed to analyze the succession of an ecosystem of microbial flora in periodontal pockets, in vitro. bacterial strains used in this study, were fusobacterium nucleatum atcc 10953, porphyromonas gingivalis 381, streptococcus sanguis atcc 10557 and streptococcus mutans omz 176. the bacterial cells, associated with beneficial, pathogenic or plaque forming activity, were cultured in brain heart infusion medium including hemin, vitamin-k1, l-cysteine hydrochloride and sodium thiogly ...19901966733
purification and characterization of a bacteroides loeschei adhesin that interacts with procaryotic and eucaryotic cells.the adhesin of bacteroides loeschei pk1295 that mediates coaggregation with streptococcus sanguis 34 and hemagglutination of erythrocytes was purified to electrophoretic homogeneity. the lectinlike protein has an estimated native mr of 450,000 and consists of six subunits of identical molecular weight (mr 75,000). the purified adhesin appears to be a basic protein with a pi between 7.4 and 8.0. amino acid and n-terminal sequence analyses were carried out with the purified protein. these indicate ...19901970562
adherence of porphyromonas (bacteroides) gingivalis to streptococcus sanguis in vitro.intergeneric bacterial adherence is responsible for the complexity of the microbiota in human dental plaque and is believed to enable some extraneous bacteria to initially colonize the human oral cavity. some current evidence indicates that streptococcus sanguis, an early colonizer of teeth, enhances subsequent colonization by porphyromonas (bacteroides) gingivalis, a bacterium associated with advanced adult periodontitis. in this study, selected strains of p. gingivalis and s. sanguis were test ...19911987021
analysis of the immunoglobulin a protease gene of streptococcus sanguis.the amino acid sequence t-p-p-t-p-s-p-s is tandemly duplicated in the heavy chain of human immunoglobulin a1 (iga1), the major antibody in secretions. the bacterial pathogen streptococcus sanguis, a precursor to dental caries and a cause of bacterial endocarditis, yields iga protease that cleaves only the pro-thr peptide bond in the left duplication, while the type 2 iga proteases of the genital pathogen neisseria gonorrhoeae and the respiratory pathogen haemophilus influenzae cleave only the p- ...19911987065
detection of immunoglobulin a1 protease-induced fab alpha fragments on dental plaque bacteria.the mechanisms by which immunoglobulin a1 (iga1) protease activity may enable bacteria to evade the effect of specific secretory iga (s-iga) antibodies are not clear. a possibility which has received indirect experimental support is that bacteria, as a consequence of the protease activity, become coated with incompetent fab alpha fragments instead of with intact antibody molecules. using a combination of nonreducing sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting, we ...19911987074
antibiotic-resistant oral streptococci in dental patients susceptible to infective endocarditis.the aim of this study was to determine the incidence of amoxycillin and erythromycin resistance in oral streptococci in patients at risk from infective endocarditis. samples of gingival crevicular flora were taken from 65 patients at the site of dental treatment, prior to the prophylactic administration of amoxycillin (54 patients) or erythromycin (11 patients). samples were also taken from 65 dental patients who were not considered to be at risk from infective endocarditis. no isolate had a min ...19911990136
association between the 65-kilodalton heat shock protein, streptococcus sanguis, and the corresponding antibodies in behçet's syndrome.the etiology of behcet's syndrome (bs) is unknown, but a number of streptococcal species have been implicated. a hypothesis was postulated that a shared antigen, such as a stress protein, might account for some of these findings. indeed, a rabbit antiserum against a 65-kda heat shock protein of mycobacterium tuberculosis revealed a corresponding 65-kda band with all six streptococcus sanguis strains examined and s. pyogenes but not with s. salivarius. by applying a panel of nine monoclonal antib ...19912004821
effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria.chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ...19912005230
aggregation of group a streptococci by human saliva and effect of saliva on streptococcal adherence to host cells.the aggregation of group a streptococci by whole, stimulated human saliva (whs) and the effect of saliva on streptococcal adherence to host cells was investigated. whs samples from 11 individuals were found to aggregate both m+ and m- group a streptococci to various degrees. the aggregating activity was sensitive to heat, edta, egta [ethylene glycol-bis(beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid], sodium dodecyl sulfate, and lipoteichoic acid. none of the simple sugars tested, mercaptoeth ...19912019436
surface hydrophobicity of small oral spirochetes.the purpose of the present study was to determine the surface hydrophobicity of small oral spirochetes and to receive an impression of the molecular organization of the cell surfaces. nine spirochete strains with one endoflagellum from each cell-end (1:2:1 spirochetes) and eight with two endoflagella from each cell-end (2:4:2 spirochetes) from subgingival plaque were examined. two hydrophobicity assay systems were used: 1) two-phase partitioning and 2) salting-out aggregation by ammonium sulfate ...19912024570
[personal identification from antibodies to oral streptococci using elisa].serum antibodies to oral streptococci were titrated by elisa for the purpose of personal identification. the bacteria concerned comprised 9 strains of streptococcus mutans, which are known to cause dental caries, and one strain of streptococcus sanguis commonly found in the oral cavity and harmless to the teeth. the quantities of igm antibodies to these streptococci were more or less dependent on the total levels of serum igm. however, no relation was found between the igg antibodies and the tot ...19912046172
evaluation of a hygiene monitor for detection of contamination in dental surgeries.routines for disinfecting working surfaces in dental surgeries are difficult to monitor without time-consuming and labour-intensive microbiological techniques, yet effective monitoring is a vital part of cross-infection control. easy to use, on-site methods would be valuable in this context. this study evaluates a portable monitor, the biotrace hygiene monitor, which uses bioluminescence to measure adenosine triphosphate (atp) on surfaces. under laboratory conditions, the ability of the monitor ...19912049220
[infectious spondylitis of the axis. apropos of a case]. 19912057734
mutacin activity of strains isolated from children with varying levels of mutants streptococci and caries.a total of 157 isolates of mutants streptococci from plaque and saliva of 94 children were studied for their serotypes, mutacin production, frequency and spectrum of activity. of these isolates 71% were identified as serotype c and 22% as serotype e. serotypes f, d and g, and one untypable strain made up about 7% of the isolates. more than one serotype was found in 13% of the children. mutacin was produced by 83% of the isolates against one or more of the 14 indicator strains representing mutant ...19912064546
synergistic degradation of mucin by streptococcus oralis and streptococcus sanguis in mixed chemostat cultures.oral streptococci can grow in mucin by utilizing the oligosaccharide chains as a source of carbohydrate. the degradation of the oligosaccharides by these species is accomplished by exoglycosidase activities. in this experiment, it was investigated whether strains from different species could cooperate in the release of sugars from the mucin oligosaccharide. to this end, streptococcus sanguis ny 584 and streptococcus oralis strain ny 586 were grown continuously in a chemically-defined medium, wit ...19912066484
[a case of kawasaki's disease combined with septicemia--isolation of streptococcus sanguis (mcls-1) and streptococcus pyogenes from blood at the acute stage].the patient was diagnosed as having kawasaki's disease. streptococci were isolated from cultures of all blood samples collected during the acute stage (the third, fifth and seventh day of the disease). the streptococci were subsequently identified as streptococcus sanguis (mcls-1) and streptococcus pyogenes. this finding may suggest induction of kawasaki's disease by s. sanguis mcls-1, and in this case, the complication of septicemia by s. pyogenes.19912066585
[an alternative approach in the surgical treatment of infectious endocarditis. the fresh antibiotic-sterilized aortic valve homograft].following the pioneering work of ross and barratt-boyes, there is currently a strong evidence based on long-term follow-up data that valve homografts are the best valve substitutes in the aortic position. currently there exists a renewed interest in the use of fresh and cryopreserved homografts as we know that homografts show a prolonged durability together with the lack of thromboembolic complications. here we present the case histories of 3 patients undergoing aortic valve replacement by fresh ...19912068371
comparison of the plaque microflora from natural and appliance-borne enamel surfaces.human enamel sections and slabs, mounted on a mandibular removable appliance, were worn by 5 adult subjects for a 1-week period. plaque was allowed to accumulate on the in situ test sites and on the adjacent natural dentition. at the end of the experimental period, the plaque microflora associated with (1) the enamel sections, (2) the enamel slabs, and (3) the acrylic base of the appliance test site was compared with that obtained from lingual and interproximal areas of the lower molar teeth. in ...19912070382
acceptor activity of affinity-immobilized dextransucrase from streptococcus sanguis atcc 10558. 19911722734
streptococcus crista sp. nov., a viridans streptococcus with tufted fibrils, isolated from the human oral cavity and throat.we studied strains of an unusual streptococcus that superficially resembles streptococcus sanguis but has fibrils that are arranged in lateral tufts. these strains were originally isolated from human throats and oral cavities and have been referred to previously as "streptococcus sanguis i," the "cr group," and the "tufted-fibril group." until now, insufficient phenotypic data have been available to allow reliable differentiation of these strains from other viridans streptococcal species, partic ...19911742199
a scheme for the identification of viridans streptococci.a collection of strains representing all the currently recognised species of oral or viridans streptococci (streptococcus sanguis, s. parasanguis, s. gordonii, s. oralis, s. mitis, s. salivarius, s. vestibularis, s. anginosus, s. constellatus, s. intermedius, s. mutans and s. sobrinus) were tested for the production of a range of glycosidase activities with 4-methylumbelliferyl-linked fluorogenic substrates, and for reactions in a range of conventional fermentation and hydrolytic tests. the resu ...19911753394
secretory iga adsorption and oral streptococcal adhesion to human enamel and artificial solid substrata with various surface free energies.in this paper, secretory iga adsorption from a single component siga solution and from human whole saliva onto human enamel and artificial solid substrata with various surface free energies was studied as a function of time. elisa indicated that screening or displacement of adsorbed siga by other salivary proteins occurred only on low surface free energy substrata, not on high surface free energy substrata such as enamel. in addition, the adhesion of three oral streptococcal strains (streptococc ...19911772830
studies on the subcellular localization of protease and arylaminopeptidase activities in streptococcus sanguis atcc 10556.intact cells of streptococcus sanguis atcc 10556 possessed arylaminopeptidases exhibiting activity toward the nitroanilide (na) derivatives of leucine, alanine, methionine, arginine, or lysine. weak hydrolytic activity was observed in assays with the na derivatives of valine, proline, glycine, or glutamic acid. subcellular localization studies revealed that arylaminopeptidase activities were located in both the cell membrane and cytoplasm. arylaminopeptidases exhibiting activity toward the leuci ...19911774382
the effect of sucralfate on the growth of cariogenic streptococci.sucralfate, an aluminum salt of sucrose octasulfate, has been shown to be effective in reducing the discomfort of radiation therapy-induced oral mucositis. this study was done to determine whether sucralfate could be used as a nutritional source for dental caries-producing organisms. three streptococcus strains were cultured in a defined medium. sucralfate powder was evaluated for its ability to be used as a carbohydrate food source by these organisms. the addition of sucralfate alone did not st ...19911774688
overwhelming postsplenectomy sepsis twenty-two years after operation risks management and prevention.a new case of fatal postsplenectomy sepsis occurring 22 years after operation for splenic rupture is described. there are now 30 published cases in which this complication occurred 10 or more years after operation. the microorganism responsible for the vast majority of cases is streptococcus pneumoniae. the causative pathogen identified in the present case was a viridans streptococcas, strep. sanguis, an opportunistic, low virulence pathogen. the clinical presentation of this patient is characte ...19911775965
[close association of streptococcus sanguis and behçet's disease].the oral flora of patients with behçet's disease was investigated and compared to that of controls. the proportion of streptococcus (s.) sanguis in the flora of patients with this disease was always higher than that of healthy controls. it is noteworthy that serotypes of s. sanguis in the oral flora of patients were different from controls. immune responses against s. sanguis were also examined in the patients. antibody titers against s. sanguis were higher in patients than in controls. furtherm ...19911776607
antimicrobial proteins in human unstimulated whole saliva in relation to each other, and to measures of health status, dental plaque accumulation and composition.saliva antimicrobial proteins may interact in a common system to influence the oral ecology. clinical studies of antimicrobial protein action thus may require a multiple-protein approach. multivariate statistical methods have been used to describe possible patterns of interaction for lysozyme, lactoferrin, salivary peroxidase and secretory iga in stimulated parotid saliva. however, oral microbes are most likely to encounter antimicrobial proteins in mixed resting saliva. relationships among leve ...19911776923
measuring harmful levels of bacteria in dental aerosols.the potential spread of the bacteria in aerosols emphasizes the familiar triad--gloves, masks and eyewear--for all operative procedures.19911783725
analogies in the two-dimensional spatial arrangement of adsorbed proteins and adhering bacteria: bovine serum albumin and streptococcus sanguis 12.neither proteins nor bacteria adsorb or adhere homogeneously to a substratum surface. the final two-dimensional spatial arrangement depends on a complicated interplay between protein-protein (bacterium-bacterium) and protein (bacterium)-substratum interactions and the prevailing hydrodynamic conditions. in this paper, results are presented of two separate experiments in which bovine serum albumin (bsa) was adsorbed to, or streptococcus sanguis 12 was deposited on substrata with different wettabi ...19911786246
the role of oral bacteria in the pathogenesis of infective endocarditis.various micro-organisms have been implicated as causative agents for bacterial endocarditis, including lactobacilli and in particular the viridans streptococci which are more commonly associated with dental caries. of these, the most frequently isolated one has the descriptive name streptococcus sanguis. the disease is characterized by growth of micro-organisms within a platelet-fibrin thrombus protruding from a valve leaflet. an understanding of the pathogenesis involves knowledge of the mechan ...19911789762
[bacteriological examination of infections in the field of oral surgery].etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...19911791332
the preparation and characterisation of proteoliposomes for targeting to oral bacteria. 19911794541
structural studies of amyloglucan and a soluble glucan produced from starch by streptococcus sanguis 1 mc 204.two alpha-d-glucans, produced from amylopectin by an oral isolate streptococcus sanguis 1 mc 204, were shown to contain both (1----4) and (1----6) linkages. the first alpha-d-glucan (amyloglucan) was adherent and highly insoluble, and methylation analysis, i.r. spectroscopy, and enzymic analyses showed it to be similar to amylopectin but less branched with longer interior and exterior chains. the second polymer was a non-adherent soluble alpha-d-glucan that was similar to amyloglucan but with lo ...19911802385
influence of delmopinol on bacterial zeta-potentials and on the colloidal stability of bacterial suspensions.delmopinol is a low molecular weight surface-active compound that has been shown to be effective against dental plaque both in vitro and in vivo and against gingivitis in vivo. to study the mode of action of delmopinol, the influence of the compound on the stability of bacterial suspensions, both with and without saliva, and on the zeta-potentials of oral streptococci was studied. the results showed that delmopinol reduced the magnitude of the zeta-potentials, but, in contrast, the colloidal sta ...19911803851
distribution, antibiotic susceptibility and tolerance of bacterial isolates in culture-positive cases of endocarditis in the netherlands.during a two-year period data were collected nationwide in the netherlands on 438 episodes of bacterial endocarditis (be) in 432 patients. of the strains isolated in these patients 419 were available for analysis. of these, 326 were isolated in native valve endocarditis (nve) and 93 in prosthetic valve endocarditis (pve). viridans streptococci, staphylococci and enterococci together constituted 87% of the isolates. more than 46% of the viridans streptococci consisted of streptococcus sanguis. en ...19911810724
polymerase chain reaction amplification, cloning, sequence determination and homologies of streptococcal atpase-encoding dnas.the highly conserved portion of the catalytic subunit (beta-subunit) of the membrane-bound, proton-translocating atpase from three strains of oral streptococci has been amplified via the polymerase chain reaction. hybridization studies demonstrated the existence of homology between escherichia coli and bacillus megaterium beta-subunit probes at the streptococcal dna level. highly degenerate primers, based on the e. coli and b. megaterium amino acid (aa) sequences, were used to amplify the homolo ...19911825305
molecular basis for the association of glucosyltransferases with the cell surface of oral streptococci.the expression of streptococcus mutans mutant glucosyltransferase-i enzymes in s. sanguis and s. milleri suggests that cell-associated glucosyltransferase activity is dependent upon both glucan synthesis and glucan binding by the carboxyl-terminal repeating units of the enzyme. mutant enzymes lacking these repeating units were only present in the extracellular fluids of these transformed streptococcal strains.19911829422
vertebral osteomyelitis secondary to an oral infection. 19911830097
failure of two macrolide antibiotics to prevent post-extraction bacteraemia.two macrolide antibiotics, erythromycin and josamycin, were compared in a double-blind trial to examine their efficacy in the prevention of post-dental extraction bacteraemia in a group of healthy patients. an in vitro blood culture system was used. isolates of streptococci were identified to species level. minimum inhibitory concentrations (mics) of erythromycin and of josamycin for each isolate were estimated by an agar dilution technique, with controls. levels of drug in the serum of voluntee ...19911832916
photolabeling of dextransucrase from streptococcus sanguis with p-azidophenyl alpha-d-glucopyranoside.dextransucrase from streptococcus sanguis atcc 10558 was photolabeled using p-azidophenyl alpha-d-glucopyranoside with an apparent rate constant of inactivation of 1.40 min-1. the dissociation constant for this compound, which acts as an acceptor molecule in the enzymatic reaction, is 90 microm. apparently two acceptor binding sites exist on dextransucrase as shown by (i.) photolabeling the enzyme with p-azidophenyl-alpha-d-[5,6-3h]glucopyranoside and (ii.) fluorescence titration experiments.19911837494
streptococcal adherence to uncoated and saliva-coated restoratives.the adherence of streptococcus sanguis and s. mutans to seven restoratives in the presence and absence of an artificial salivary pellicle has been investigated. the physiochemical surface characteristics of the bacteria and of the restoratives were also measured, together with the effect of salivary coating of restoratives. zeta potential of uncoated restoratives showed a positive correlation with the number of adherent s. mutans cells and s. sanguis cells. s. sanguis showed a positive correlati ...19911839312
clinical and microbiological findings on newly inserted hydroxyapatite-coated and pure titanium human dental implants.the clinical and microbiologic features of 30 hydroxyapatite-coated root-form endosseous dental implants (tri-stage) were compared to 10 similar pure titanium implants without hydroxyapatite coatings. in 7 of 9 partially edentulous patients studied, pure titanium fixtures were placed adjacent to hydroxyapatite-coated implants. implants in the maxilla were submerged beneath mucosal tissues after implant placement for a minimum of 6 months, and in the mandible for at least 4 months. all patients w ...19911843465
sequence and structural analysis of surface protein antigen i/ii (spaa) of streptococcus sobrinus.streptococcal antigen i/ii or the surface protein antigen a (spaa) of streptococcus sobrinus is an adhesin which mediates binding of the organism to tooth surfaces. the complete sequence of the gene which encodes spaa has been determined. the gene consists of 4,584 bp and encodes a protein of 1,528 amino acid residues. the deduced amino acid sequence shows extensive homology with those of the cell surface adhesins from streptococcus mutans serotypes c and f and from streptococcus sanguis. struct ...19911855987
molecular cloning and nucleotide sequencing of the arthrobacter dextranase gene and its expression in escherichia coli and streptococcus sanguis.a bacterial strain, which assimilated dextran and water-insoluble glucan produced by streptococcus mutans, was isolated from soil. the bacterium produced and secreted potent dextranase activity, which was identified as arthrobacter sp. and named cb-8. the dextranase was purified and some enzymatic properties were characterized. the enzyme efficiently decomposed the water-insoluble glucan as well as dextran. a gene library from the bacteria was constructed with escherichia coli, using plasmid puc ...19911859672
incidence of penicillin tolerance among blood culture isolates of streptococcus sanguis, 1987-88.laboratories that reported isolations of streptococcus sanguis from blood cultures to the communicable disease surveillance centre (cdsc) colindale were requested to submit strains to bath public health laboratory to allow the prevalence of penicillin tolerance within different biotypes of this species to be studied. one hundred and fifty one streptococcus spp were received from 78 united kingdom laboratories in one year. strains were identified using the api 20 strep, and minimum inhibitory con ...19911864989
interactions of sanguinarine and zinc on oral streptococci and actinomyces species.sanguinaria extract, which contains benzophenanthridine alkaloids, has been used as a folk medicine for many years. minimum inhibitory and minimum bactericidal concentrations (mic and mbc values) for sanguinarine were determined for common and etiologically important plaque bacteria. because the efficacy of sanguinarine is believed to be enhanced by zinc, isobolograms were assessed to determine their mode(s) of interaction. hydrogen ion concentration influenced the inhibitory activity of both sa ...19911878916
delineation of a segment of adsorbed salivary acidic proline-rich proteins which promotes adhesion of streptococcus gordonii to apatitic surfaces.cells of several strains of streptococcus gordonii attached in much higher numbers to experimental pellicles formed from samples of submandibular or parotid saliva on hydroxyapatite (ha) beads than to buffer controls. the nature of the salivary components responsible were investigated by preparing experimental pellicles from chromatographic fractions of submandibular saliva obtained from trisacryl gf 2000m columns. adhesion of s. gordonii blackburn was promoted by two groups of fractions. the ad ...19911879920
salivary-agglutinin-mediated adherence of streptococcus mutans to early plaque bacteria.interspecies binding is important in the colonization of the oral cavity by bacteria. streptococcus mutans can adhere to other plaque bacteria, such as streptococcus sanguis and actinomyces viscosus, and this adherence is enhanced by saliva. the salivary and bacterial molecules that mediate this interaction were investigated. salivary agglutinin, a mucinlike glycoprotein known to mediate the aggregation of many oral streptococci in vitro, was found to mediate the adherence of s. mutans to s. san ...19911894355
subclass distribution of salivary secretory immunoglobulin a antibodies to oral streptococci.the ability of specific secretory immunoglobulin a (s-iga) antibodies to inhibit bacterial colonization of mucosal surfaces may be neutralized by the activity of bacterial iga1 proteases. because of the resistance of the iga2 subclass to these enzymes, the biological effect of iga1 proteases in vivo may depend on the subclass distribution of the bacterium-specific antibodies. we have estimated the subclass distribution of s-iga antibodies in saliva samples from 13 individuals against iga1 protea ...19911894364
[non-groupable streptococci: identification, sensitivity to antibiotics (charles nicolle hospital in tunis)].50 strains of viridans streptococci isolated from human material are identified by biochemical tests (bile esculin, cl na 6,5%, acid production from lactose, mannitol, sorbitol, inulin, arginine, esculin and starch hydrolysis; production of levan and dextran in sucrose media) streptococcus salivarius and streptococcus mitis are predominant species. susceptibility to antibiotics was studied: 70% of viridans streptococci were susceptible to all antibiotics tested, high level resistance to aminogly ...19911895917
on salivary film formation and bacterial retention to solids. a methodological and experimental in vitro study.a parallel-plate flow cell system was developed to assess individual and combined parameters relevant to oral microbial retention. in this system, internal reflection germanium prisms represented oral solid surfaces including tooth surfaces. the system used, allowed adsorbed protein films to be analyzed in situ by multiple non-destructive surface analytical techniques, without provoking surface associated biofilms. using a range of biochemical and biophysical techniques, germanium prisms of medi ...19911896924
sorbitol transport and metabolism by oral streptococci.sorbitol transport by oral streptococci was mediated by a phosphoenolpyruvate phosphotransferase system (pts). the transition of s. sanguis 160 from continuous growth on limiting glucose to limiting sorbitol resulted in induction of eiisor of the sorbitol-pts, as well as sorbitol-6-p dehydrogenase which converts sorbitol-6-p to fructose-6-p. sorbitol transport activity required the presence of a soluble sorbitol-specific component of the pts, tentatively identified as an enzyme iii for sorbitol ...19911896926
platelet aggregation induced by uncommon serotypes of streptococcus sanguis isolated from patients with behçet's disease.uncommon serotypes were tested for their ability to induce aggregation in platelet-rich plasma. both uncommon and common serotypes induced platelet aggregation in rabbit platelet-rich plasma, but serotonin release was higher with the uncommon serotypes. aggregation at atp release varied between uncommon and common serotypes. with human platelet-rich plasma, only uncommon serotypes showed aggregation. differences in serotype selectivity and the rate of aggregation were noted among platelet donors ...19911898270
streptococcus sanguis survival in k-sol. comparison of gentamicin and the fluoroquinolone antibiotics.viridans streptococci are poorly covered by gentamicin sulfate in corneal storage medium. to evaluate possible antibiotic alternatives among the newer broad-spectrum fluoroquinolone antibiotics, we compared the survival of the viridans representative streptococcus sanguis in k-sol with gentamicin sulfate (100 mg/l), norfloxacin (250 mg/l), ciprofloxacin lactate (250 mg/l), ofloxacin (250 mg/l), or no antibiotic. at 23 degrees c, k-sol with gentamicin produced a 2-log kill by 80 minutes. by compa ...19911901486
identification of a region that influences host range of the streptococcal conjugative plasmid pip501.pip501 is a member of a group of conjugative plasmids that are self-transmissible to a wide variety of streptococci as well as to other gram-positive bacteria. several pip501 restriction fragment deletion derivatives have been isolated and characterized. in this paper we describe one such derivative (pva1702) which was conjugally proficient but had a limited host range. the loss of host range ability was seen as decreased conjugal transfer from enterococcus faecalis to streptococcus sanguis and ...19911903546
infectious endocarditis after fiberoptic sigmoidoscopy. with a literature review.infectious endocarditis is a feared complication of procedures causing bacteremia. gastrointestinal procedures cause bacteremia, but are seldom followed by infectious endocarditis. of nine cases found in the english literature, only five have convincing evidence that endocarditis resulted from the gastrointestinal procedure. i present a new case of endocarditis due to streptococcus sanguis type ii after fiberoptic sigmoidoscopy.19911918852
protein a-streptokinase fusion protein for immunodetection of specific igg antibodies.the streptococcal streptokinase gene truncated at its 5' end was fused to regions of the staphylococcal protein a gene encoding the fc-binding domains a and b. the resultant fusion gene, when expressed in the escherichia coli lacpo system or under the spea expression/secretion signals in s. sanguis, specified a bifunctional hybrid protein, spa-skc, capable of fc binding and plasminogen activation. when used in immunoassays designed to titrate antisera raised against bovine chymosin, human serum ...19911919031
[alpha-hemolytic streptococcal septicemia and meningitis in immunocompromised children].many kinds of microorganisms can produce toxic septicemia in immunocompromised hosts. we are reporting alpha-hemolytic streptococcal septicemia and meningitis in two children with hematological malignancies. [case 1] 6 year old girl who had been suffering from acute lymphocytic leukemia. she had sepsis and bacterial meningitis in maintenance-therapy for leukemia. streptococcus sanguis was isolated from the blood and cerebrospinal fluid (csf). [case 2] 11 year old girl who had had malignant lymph ...19911919121
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