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treatment of extraoral sinus tracts from traumatized teeth with apical periodontitis.when a draining lesion is encountered on the skin of the face, an endodontic origin should always be considered in differential diagnosis. non-surgical endodontic therapy, sometimes complimented by surgery, or extraction are the choices for the treatment of these cases. three cases of extraoral sinus tract on the chin caused by necrotic pulp of traumatized lower anterior teeth are presented. a paste consisting of calcium hydroxide and barium sulfate powder mixed with glycerin was used. usage of ...19957641626
bacteremia in conjunction with endodontic therapy.this study characterizes oral microorganisms believed to have spread from the root canal into the blood stream during and after endodontic therapy of teeth with asymptomatic apical periodontitis. microbiological samples were taken under aseptic conditions from the root canal of 26 single-rooted teeth in 26 patients. in the endodontic treatment of 13 of the patients (group 1), the first 3 reamers, sizes 15, 20 and 25, were used to a level 2 mm beyond the apical foramen. in the other 13 patients ( ...19957641631
microbiology of gastrostomy site wound infections in children.specimens of pus were obtained from gastrostomy site wound infections in 22 children. polymicrobial flora was found in 21 of the 22 wounds. aerobic or facultative bacteria only were isolated in eight (36%) instances and mixed aerobic-anaerobic flora were isolated from the other 14 (64%) wounds. a total of 102 bacterial isolates (57 aerobic and 45 anaerobic) and seven cultures of candida were obtained. the most frequent isolates were escherichia coli (16 isolates). peptostreptococcus spp. (14), e ...19957650731
multiple ligand interactions for bacterial immunoglobulin-binding proteins on human and murine cells of the hematopoetic lineage.a group of bacterial ig-binding surface proteins were studied: protein h and m1 are from streptococcus pyogenes and interact with igg, protein l is expressed by peptostreptococcus magnus and shows affinity for ig light chains, whereas protein lg is a chimeric construction combining the binding properties of protein l with the igg-binding activity of protein g from group c and g streptococci. proteins l and h coupled to sepharose were mitogenic for human peripheral blood lymphocytes (pbl) and mou ...19957660068
endoscopic treatment of acute frontal sinusitis: indications and limitations. 19957675495
[bone and joint infections due to unusual pathogens].between 1989 and 1993, 7 patients--2 men and 5 women from 19 to 70 years of age--with osteomyelitis due to an unusual organism were observed. 3 cases with salmonella typhimurium and one at a time with escherichia coli, peptostreptococcus, propionibacterium acnes and pasteurella multocida. 5 patients were treated operative accompanied with antibiotics and 1 without operation. 1 patient declined the indicated surgical therapy. the outcome were total healing in 3 cases, healing with ankylosis of th ...19957754663
necrotizing fasciitis of the head and neck with intrathoracic extension.we present the case history of a white male patient who initially presented in the emergency department with a peritonsillar abscess and subsequently returned several days later with necrotizing cervical fasciitis with intrathoracic extension. the successful treatment, utilizing surgical debridement and hyperbaric oxygen, will be discussed for this disease, which causes up to 50% or greater mortality.19957769648
[the prospects for using the new macrolide antibiotic azithromycin (sumamed) in the combined treatment of periodontitis].antibacterial activity of sumamed, a macrolide antibiotic, in respect of anaerobic microflora of the oral cavity, such as bacteroides, prevotella, porphyromonas, fusobacterium, peptostreptococcus, str. sanguis and staph. aureus, was assessed. the drug was found to be highly effective in respect of bacteroids (mpc90: 1,4 to 30 mg/mlmg/ml, this permitting the use of sumamed in the treatment of 9 patients with chronic generalized periodontitis in the exacerbation stage. bacteriological and clinical ...19957770869
crystalline surface protein of peptostreptococcus anaerobius.the surface ultrastructure of three anaerobic gram-positive cocci frequently encountered in oral infections, peptostreptococcus micros, p. magnus and p. anaerobius, was studied. the type strains of p. micros (dsm 20468) and p. anaerobius (atcc 27337), several clinical isolates of both species and the type strain of p. magnus (dsm 20470) were included. thin-sectioned cells studied by electron microscopy revealed a homogeneous layer outside the peptidoglycan layer in p. anaerobius. in p. micros an ...19957773401
timing of surgery in patients with infected spinal dermal sinuses: report of two cases.in the past, if infection was present at the time a spinal dermal sinus was discovered, the sinus opening and cerebrospinal fluid were sterilized with a course of appropriate antibiotic therapy prior to the surgical intervention. the cases of two children with an infected spinal dermal sinus are reported here. one patient developed acute paraplegia during the initial stage of antibiotic therapy, and the second patient, who was admitted only with signs of fever and irritability, experienced after ...19957773970
periodontal findings in spouses. a clinical, radiographic and microbiological study.clinical, radiographic and microbiological examination of periodontal conditions was carried out in 2 groups of married couples to assess similarities between husband and wife. the diseased probands (n = 10) exhibited advanced periodontitis and the healthy ones (n = 10) were periodontally normal. the clinical examination comprised the assessment of plaque, probing pocket depths, gingival bleeding on probing, suppuration, supragingival and subgingival calculus. the extent and type of alveolar bon ...19957775676
sinusitis and bronchial asthma in children.sinus disease has been assumed to exist in children with respiratory tract allergies. we consecutively evaluated 375 patients (245 male, 130 female) with childhood onset asthma, between 5 and 15 years of age (mean 7.8 years of age). abnormal waters radiographs were found in 205 patients (54.7%) which included mucosal wall thickening greater than 6 mm (67.3%), complete opacification (22.9%) and air-fluid levels (9.8%). the level of immunoglobulin (ig) e was found to be much higher in non-sinusiti ...19957778440
role of anaerobic bacteria in chronic otitis media and cholesteatoma.otitis media (om), a common infection in children, can cause significant morbidity. selection of the most appropriate treatment regimen directed against the pathogens responsible for the om can minimize complications. the most frequently isolated bacteria from chronic om are staphylococcus aureus, pseudomonas aeruginosa and anaerobic bacteria. the predominant anaerobes are peptostreptococcus spp., pigmented prevotella and porphyromonas spp., bacteroides spp. and fusobacterium spp. many of the or ...19957782173
intracranial complications of sinusitis in childhood.complications of sinusitis in children, such as intracranial abscess formation, are uncommon and are often clinically unremarkable in comparison to similar disease processes in adults. between 1983 and 1991, 443 children were admitted to children's national medical center in washington, dc, for treatment of sinusitis. fourteen of these children presented with intracranial extension of the infection and abscess formation. a retrospective review of these patients revealed that the risk of developi ...19957717619
microbiology of chronic suppurative otitis media in children in surabaya, indonesia.the aerobic and anaerobic microbiology of 38 children from surabaya, indonesia, who suffered from chronic suppurative otitis media (csom) was studied using strict microbiological methodology. a total of 106 isolates (49 anaerobic and 57 aerobic) were recovered. aerobic organisms alone were isolated from 11 (29%), anaerobic bacteria only in 4 (11%) and mixed aerobic and anaerobic flora were present in 23 (60%). the predominant organisms were peptostreptococcus sp., prevotella sp., bacteroides sp. ...19957729990
clinical and microbiological features of necrotizing fasciitis.the microbiological and clinical characteristics of 83 patients with necrotizing fasciitis (nf) treated over a period of 17 years are presented. bacterial growth was noted in 81 of 83 (98%) of specimens from patients with nf. aerobic or facultative bacteria only were recovered in 8 (10%) specimens, anaerobic bacteria only were recovered in 18 (22%) specimens, and mixed-aerobic-anaerobic floras were recovered in 55 (68%) specimens. in total, there were 375 isolates, 105 aerobic or facultative bac ...19957494032
budgerigar breeders' hypersensitivity pneumonitis presenting as chronic bronchitis with purulent sputum.a 49-year-old woman complained of cough and a purulent sputum (about 40 ml/day). she kept budgerigars for a period of 30 years, she first noticed a productive cough 10 years previously. her chest roentgenogram revealed a diffuse reticulonodular pattern. she was diagnosed as having a chronic type of budgerigar breeder's lung associated with chronic bronchitis. peptostreptococcus anaerobius was cultured from her sputum, bronchoalveolar lavage fluid, as well as from droppings of her budgerigar. thi ...19957496084
[a case of gas-producing brain abscess with subdural empyema: timing of the operation for brain abscess].a case of gas-producing brain abscess with subdural abscess was reported. an 18-year-old boy was admitted with a five-day history of vomiting and high grade pyrexia. plain skull roentgenograms demonstrated left frontal multiple gas bubbles. ct scan and mri showed that both brain and subdural abscesses contained gas in the left frontal area. antibiotics and glyceol were intravenously administrated. in serial ct scans, subdural abscess was not recognized, while brain abscess was enhanced in a ring ...19957477705
macrolides resistance of common bacteria isolated from taiwan.to determine the susceptibility to macrolides of common pathogenic bacteria isolated from taiwan, the in vitro activities of erythromycin, roxithromycin, azithromycin, clarithromycin, and dirithromycin were tested against 492 clinical isolates of eight different bacteria, collected from the national taiwan university hospital. the results showed high minimum inhibitory concentrations (mics) against most of the tested bacteria. the mic90s for staphylococcus aureus (both methicillin-resistant and ...19959407220
a study of the anaerobic bacterial flora of the female genital tract in health and disease.semi-quantitative and qualitative bacterial assessment of the vaginal and cervical flora of a total of 202 women was carried out over a period of six months to determine the bacterial flora in three groups of women and changes caused by prior use of antibiotics. the number was made up of 32 healthy volunteers, 80 women with gynaecological problems and 90 women with gynaecological infections who had had antibiotic treatment prior to this study. standard methods were used for the investigations. f ...19958907604
[aerobic and anaerobic bacterial flora in acute abdominal illnesses].366 specinmens taken from patients suffering from acute abdominal illnesses who had undergone operations were examined. bacterial pathogens were isolated from 318 specimens. aerobic bacteria were isolated from 53.8% specimens. anaerobic bacteria were isolated from 12.6% specimens. mixed bacterial flora were isolated from 33.6% specimens. among aerobic bacteria e. coli and streptococcus faecalis were most often isolated. among anaerobic bacteria bacteroides fragilis, peptostreptococcus spp. and p ...19958833932
advanced periodontitis associated with larsen's syndrome: case report.larsen's syndrome is a genetic disorder of connective tissue characterized by multiple large-joint dislocations, abnormalities of hands and feet, and anomalous facial features. although findings involving multiple systems have been added to its clinical and pathological entity, oral pathology besides cleft palate has not been reported. we report here advanced periodontitis with severe gingival inflammation in a 29-year-old larsen's syndrome patient. bacterial culture of subgingival plaque identi ...19957899108
bioactivation of cysteine conjugates of 1-nitropyrene oxides by cysteine conjugate beta-lyase purified from peptostreptococcus magnus.to determine the role of cysteine conjugate beta-lyase (beta-lyase) in the metabolism of mutagenic nitropolycyclic aromatic hydrocarbons, we determined the effect of beta-lyase on the mutagenicities and dna binding of cysteine conjugates of 4,5-epoxy-4,5-dihydro-1-nitropyrene (1-np 4,5-oxide) and 9,10-epoxy-9,10-dihydro-1-nitropyrene (1-np 9,10-oxide), which are detoxified metabolites of the mutagenic compound 1-nitropyrene. we purified beta-lyase from peptostreptococcus magnus gai0663, since p. ...19958526486
structures of bacterial immunoglobulin-binding domains and their complexes with immunoglobulins.three-dimensional structures are now available for several immunoglobulin binding domains from bacterial proteins a, g, and l. x-ray diffraction and nmr experiments on complexes of these domains with portions of immunoglobulins have revealed common structural themes used in these interactions. these data expand our understanding of structure/function relationships in these molecular recognition processes and provide the basis for rational design of artificial immunoglobulin-binding molecules.19958528763
salivary levels of suspected periodontal pathogens in relation to periodontal status and treatment.the primary ecological niche for suspected periodontal pathogens seems to be the subgingival area, even though periodontal pathogens are also frequently recovered from saliva. the interrelationship of different periodontal conditions and the salivary levels of suspected periodontal pathogens is not known. in the present study, salivary levels of actinobacillus actinomycetemcomitans, porphyromonas gingivalis, prevotella intermedia, campylobacter rectus, and peptostreptococcus micros were determin ...19958530742
competition for peptides and amino acids among periodontal bacteria.we recently studied the utilization of glutathione (l-gamma-glutamyl-l-cysteinylglycine), l-cysteinylglycine and l-cysteine by anaerobic bacteria. the rate of hydrogen sulfide formation from these compounds was determined and it was concluded that peptostreptococcus micros and fusobacterium nucleatum subsp. nucleatum had an active transport of small peptides. in the present study it is shown that methyl mercaptan formation from l-methionine and l-methionyl-containing peptides can also be used to ...19958544102
surgical sepsis: constancy of antibiotic susceptibility of causative organisms.it is well documented that antibiotic therapy exerts selective pressure on bacteria. conversion of bacteria from susceptible to resistant to antibiotics has been observed often during antimicrobial therapy. it has been postulated that human intestinal reservoirs facilitate communication of transposons that can transfer resistance determinants among various bacterial species.19957740421
adherence of bacteria to intraocular lenses: a prospective study.the study was designed to investigate the bacterial flora of the operating field during routine cataract surgery and the source of intraocular lens contamination during the surgery.19957742281
microbiological features and pathogenesis of peritonsillar abscesses.samples of pus aspirated from 53 peritonsillar abscesses were examined in detail for aerobic and anaerobic bacteria, and the microbiological results correlated with clinical data in 44 cases. in 45 samples (85%) cultures were positive: 7 yielded organisms consistent with an aerobic infection, mainly lancefield group a beta-haemolytic streptococci (5/7), and 38 yielded organisms consistent with an anaerobic infection. the anaerobic infections were usually mixed, but in two cases fusobacterium nec ...19958605900
endodontic pathogens: propagation of infection through patent dentinal tubules in traumatized monkey teeth.periapical pathology indicating endodontic infection, when present in marginal periodontitis-affected teeth, has recently been shown to be an aggravating factor in progression of marginal destruction. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endo ...19958625937
non-sporing anaerobes in certain surgical group of patients.the magnitude of non-sparing anaerobic (nsa) infections has been defined in the postoperative wounds on colorectum in children (57.1%), general surgery (0%), abdominoperineal and uterocervical operations (11-45%) in gynaecologic and obstetrical cases and perforative peritonitis (25.8-32.3%). children below the 6 months age group bear less risk of acquiring nsa infection. under certain situations, metronidazole combats nsa infections in a better way than other antibacterials. the bacteriology of ...19958648152
pneumonia in mechanically ventilated children.the quantitative aerobic and anaerobic microbiology of bronchial aspirates, obtained using protective brush catheters, from 10 children with ventilator-associated pneumonia, is presented. aerobic or faculative organisms only were isolated in 1 child, anaerobic bacteria only in 3, and aerobic mixed with anaerobic bacteria in 6. there were 10 aerobic or faculative and 17 anaerobic isolates. the predominant aerobes were pseudomonas aeruginosa (2 isolates) and klebsiella sp. (2). the predominant ana ...19958685643
the in vitro activity of trospectomycin against anaerobic bacteria.the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...19958667035
crystallization and x-ray analysis of a single fab binding domain from protein l of peptostreptococcus magnus.protein l is a multidomain cell wall constituent of certain strains of peptostreptococcus magnus which binds to the variable domain of immunoglobulin kappa-light chains. a single immunoglobulin-binding domain of m(r) = 9000 from this protein has been isolated and crystallized. the crystals are of space group p4(2)2(1)2, with cell dimensions a = b = 66.9 a, c = 68.3 a, and diffract to at least 2.2 a resolution. the asymmetric unit of the crystal contains two molecules of the protein l domain, rel ...19958749861
cloning, expression and purification of ppl-1, a kappa-chain binding protein, based upon protein l from peptostreptococcus magnus.protein l is a multi-domain, cell wall constituent of certain strains of peptostreptococcus magnus, which binds to the variable domain of the l-chains of ig. a gene fragment which codes for a single ig-binding domain of protein l (ppl-1) was cloned into a modified pkk223-3 vector and over-expressed in e. coli jm103. a rapid protein purification protocol is described. in these studies, purified ppl-1 was immobilised on to an agarose gel and tested against an array of igs and ig fragments. it was ...19958767928
a risk-benefit assessment of teicoplanin in the treatment of infections.teicoplanin is a glycopeptide antibiotic whose activity is selectively oriented against gram-positive aerobic and anaerobic bacteria, including staphylococcus aureus, coagulase-negative staphylococci, clostridium difficile, peptostreptococcus spp. and corynebacterium jeikeium; such activity is affected by neither methicillin resistance nor beta-lactamase production. teicoplanin is not significantly absorbed from the gastrointestinal tract; consequently, it has to be administered intravenously (e ...19958785019
treatment of peptostreptococcus micros endocarditis with teicoplanin. 19958562764
a case of anaerobic pericarditis. 19958562770
endodontic pathogens in periodontal disease augmentation.periapical pathology indicating endodontic infection, when present in periodontitis-affected teeth, has recently been shown to be correlated to marginal periodontal breakdown. this has been associated with patency of dentinal tubules in the tooth cervix, an area normally devoid of cementum following periodontal therapy. these studies are, however hampered by that only circumstantial evidence such as presence of periapical destruction have been applied as criteria of endodontic infection. the aim ...19958583015
oral microbiota in subjects with a weak or strong response in experimental gingivitis.the purpose of the present study was to examine the composition of the oral microbiota in subjects who had previously demonstrated to develop either a weak or strong response to experimental gingivitis. for this study, subjects were selected from a pool of 25 individuals who had participated twice in an experimental gingivitis trial. out of these 25 panellists, 6 subjects were selected who had developed 2x a weak gingival inflammatory response and 7 subjects who had developed 2x a strong gingiva ...19958583022
phylogenetic analysis of ruminococcus flavefaciens, the type species of the genus ruminococcus, does not support the reclassification of streptococcus hansenii and peptostreptococcus productus as ruminococci.the 16s rrna gene sequence of the type strain of ruminococcus flavefaciens, the type species of the genus ruminococcus, was determined by pcr direct sequencing. a comparative sequence analysis showed that r. flavefaciens is phylogenetically related to a small cluster (cluster iv of collins et al. [m. d. collins, p. a. lawson, a. willems, j. j. cordoba, j. fernandez-garayzabal, p. garcia, j. cai, h. hippe, and j. a. e. farrow, int. j. syst. bacteriol. 44:812-826, 1994]) of organisms which include ...19958590686
antimicrobial and toxic effects of established and potential root canal irrigants.three dilutions of the commonly used irrigant sodium hypochlorite (0.5%, 2.5%, and 5.25%) and the potential irrigants peridex, chlorhexidine gluconate (0.12%), and therasol were tested. alcohol and saline served as controls also. the in vitro portion of this study used four different microorganisms (streptococcus mutans, peptostreptococcus micros, prevotella intermedius, and porphyromonas gingivalis) to determine antimicrobial effect. presterilized 7-mm diameter whatman paper disks soaked with t ...19958596073
pet findings in a brain abscess associated with a silent atrial septal defect.brain abscesses are classical complications of congenital heart disease (chd) in children and adolescents. this association is rarely observed in adults. we report a 46-year-old man presenting a fronto-parietal abscess associated with an asymptomatic atrial septal defect. positron emission tomography (pet) study revealed high uptake of l-[methyl-11c]methionine ([11c]methionine) and 2-[18f]fluoro-2-deoxy-d-glucose (fdg) around the brain abscess. we suggest (1) to exclude a silent cardiac malforma ...19958599907
antimicrobial activity of sch 27899, oligosaccharide member of the everninomycin class with a wide gram-positive spectrum.the in vitro antimicrobial activity of sch 27899 (everninomycin), a novel oligosaccharide compound of the everninomycin class, was compared with vancomycin, chloramphenicol, clinafloxacin, teicoplanin and doxycycline against 428 clinical strains of bacteria. everninomycin base exhibited the greatest antimicrobial activity compared to other formulations against all strains tested (mic90: 0.25 microg/ml) followed by clinafloxacin and teicoplanin (mic90: 0.5 microg/ml), vancomycin (mic90: 2 microg/ ...199511866719
the aerobic and anaerobic microbiology of pustular acne lesions.specimens from 32 pustular acne lesions that were inoculated on media supportive for the growth of aerobic and anaerobic bacteria showed bacterial growth. only aerobic or facultative bacteria were recovered in 15 (47%) specimens, only anaerobic bacteria in 11 (34%) specimens, and mixed aerobic and anaerobic bacteria in 6 (18%) specimens. a total of 57 isolates, 31 anaerobes (1.0 per specimen) and 26 aerobes (0.8 per specimen) were recovered. the predominant isolates were staphylococcus sp. (19 i ...199516887540
nonpuerperal breast infection.we undertook a microbiological study of purulent specimens from women with symptomatic breast abscesses.199518476022
bacteriology and beta-lactamase activity in acute and chronic maxillary sinusitis.to assess the bacteriology and beta-lactamase enzyme activity in aspirates of 10 acutely and 13 chronically inflamed maxillary sinuses.19968600928
microbiology of mediastinitis.to study the microbiologic and clinical characteristics of patients with mediastinitis.19968572845
identification and discrimination of oral asaccharolytic eubacterium spp. by pyrolysis mass spectrometry and artificial neural networks.curie-point pyrolysis mass spectra were obtained from 29 oral asaccharolytic eubacterium strains and 6 abscess isolates previously identified as peptostreptococcus heliotrinreducens. pyrolysis mass spectrometry (pyms) with cluster analysis was able to clarify the taxonomic position of this group of organisms. artificial neural networks (anns) were then trained by supervised learning (with the back-propagation algorithm) to recognize the strains from their pyrolysis mass spectra; all eubacterium ...19968574131
[in vitro activities of sulopenem, a new parenteral penem, against anaerobes].in vitro activities of sulopenem, a novel parenteral penem, was compared with those of imipenem, flomoxef, cefuzonam, cefoperazone and sulbactam/ampicillin against 66 reference strains (19 genera, 61 species) and 392 recent clinical isolates of anaerobic bacteria and fastidious aerobic bacteria. sulopenem had a very broad spectrum against anaerobic bacteria. in general, this compound was active against anaerobic reference strains with mics of < or = 0.78 micrograms/ml, while being the least acti ...19968786627
susceptibilities of bovine summer mastitis bacteria to antimicrobial agents.the susceptibility to 9 antimicrobial agents of 32 aerobic bacterial isolates and to 10 antimicrobial agents of 37 anaerobic bacterial isolates from 23 cases of bovine summer mastitis (16 actinomyces pyogenes isolates, 8 streptococcus dysgalactiae isolates, 3 s. uberis isolates, 3 s. acidominimus isolates, 2 streptococcus spp., 15 peptostreptococcus indolicus isolates, 10 fusobacterium necrophorum isolates, and 12 isolates of anaerobic gram-negative rods) was determined by the agar dilution meth ...19968787898
microbiology of acute purulent pericarditis. a 12-year experience in a military hospital.to study the aerobic and anaerobic micro-biological and clinical characteristics in 15 cases of acute pericarditis treated over a 12-year period.19968790081
microbiology of periapical abscesses and associated maxillary sinusitis.aspirate of pus from 5 periapical abscesses of the upper jaw and their corresponding maxillary sinusitis were studied for aerobic and anaerobic bacteria. polymicrobial flora were found in all instances, where the number of isolates varied from 2 to 5. anaerobes were recovered from all specimens. the predominant isolates were prevotella sp., porphyromonas sp., fusobacterium nucleatum, and peptostreptococcus sp. concordance in the microbiological findings between periapical abscess and the maxilla ...19968794972
clinical, microbiological and immunological profile of healthy, gingivitis and putative active periodontal subjects.thirteen periodontally healthy subjects were monitored clinically for 6-12 months. clinical measurements at 6-weekly intervals included duplicate pd measurements, presence of plaque, redness, and bleeding on probing. baseline measurements consisted of 2 visits 1 wk apart. microbial samples were taken from 11 of the subjects who had completed at least 8 months of monitoring. levels of serum antibodies to 12 periodontal species were determined from 10 subjects. standard deviations of replicate pd ...19968814590
bacteriologic findings in tonsillitis and pericoronitis.bacteriologic samples from 31 young men were cultured quantitatively for aerobes and anaerobes; these samples included 31 specimens of tonsils (16 infected and 15 healthy), 16 specimens from pericoronal pockets of lower third molars (11 infected and 5 symptom-free), and 6 postoperative specimens from lower-third-molar extraction sockets. anaerobes were isolated more often from infected third molars than from infected tonsils (14.5 isolates vs. 8.4 isolates, respectively; p < .001). infected tons ...19968816129
amplified expression and large-scale purification of protein l'.the gene fragment (ppl') encoding the functional unit of peptostreptococcus protein l was isolated using pcr and expressed in e. coli. as the gene fragment lacked its own promoter, the 5' pcr primer was designed to incorporate an nde1 restriction site (catatg) into the gene. this enabled the gene to be cloned in frame into an nde1 restriction site immediately downstream of a trp promoter. to prevent read through, a stop codon was introduced into the 3' primer. expression of ppl' was up to 27% to ...19968818265
a purgative action of barbaloin is induced by eubacterium sp. strain bar, a human intestinal anaerobe, capable of transforming barbaloin to aloe-emodin anthrone.orally administered barbaloin (100 mg/kg) did not induce any diarrhea in male wistar rats, in spite of severe diarrhea with sennoside b (40 mg/kg). also, in gnotobiote rats mono-associated with peptostreptococcus intermedius, a human intestinal anaerobe capable of reducing sennidins to rhein anthrone, barbaloin did not induce diarrhea; the faecal water content (71.9%) 8 h after the administration of barbaloin was not increased, compared with that (73.9%) just before the treatment. however, sever ...19968820926
changing anaerobic spectrum in suppurative lung disease: a case report.a spectrum of three different anaerobes were isolated from a debilitated patient with suppurative lung disease, within a two-year period. repeated isolation from three consecutive samples and symptomatic relief with metronidazole provide clinical evidence of anaerobic lung infection. this case emphasizes the importance of anaerobic culture in cases of protracted pulmonary suppurative disease.19968822645
[antimicrobial activities of tosufloxacin against recently obtained clinical isolates].to evaluate antimicrobial activities of tosufloxacin (tflx), minimum inhibitory concentrations (mics) of tflx and other drugs were determined against clinical isolates that were obtained in our laboratory from february of 1993 to january of 1994 (period i), and from february of 1995 to january of 1996 (period ii). the results are summarized as follows: 1. compared to mic90's reported by others for tflx in the 1980's, those obtained by us against beta-streptococci, streptococcus pneumoniae, pepto ...19968828073
the dynamics of microbial colonization of barrier membranes for guided tissue regeneration.the microbial colonization of expanded polytetrafluoroethylene membrane by putative periodontopathogens at 3 minutes of intraoral manipulation was determined in 42 patients with 42 mandibular posterior two- to three-wall defects. twenty patients exhibited no periodontal pockets of > or = 5 mm, other than the study site, and low levels of pathogens (group a). twenty-two patients revealed multiple periodontal pockets of 5 mm or more and numerous pathogens (group b). within the preceding 3 months o ...19968832481
bacteriological epidemiology and treatment of bacterial vaginosis.one hundred symptomatic women with clinical bacterial vaginosis (bv) were enrolled in this study. the bacteriological epidemiology of bv and efficacy of oral or vaginal administration of antimicrobial agents for treatment of bv were investigated. the epidemiology of bv was investigated before antimicrobial therapy. fifty patients were treated with oral administration of metronidazole (mtn), 500 mg twice a day, and 50 patients with 2% clindamycin (cldm) phosphate in a vaginal cream, 5 g once a da ...19968751270
polymerase chain reaction-based detection of bacteria in semen.to determine if the presently used bacterial detection techniques provide accurate and complete profiles of microorganisms found in human semen.19968751749
[antibiotic sensitivity of anaerobic bacteria isolated during one year (1993)].the aim of this study was to determine the antimicrobian susceptibility of the anaerobe bacteria isolated from clinical samples in the authors' department in 1993.19968756216
[in vitro activity of a new fluoroquinolone, marbofloxacin (ro 09-1168) against strictly anaerobic bacteria and some bacteria from human fecal flora].as recommended by the eec, the in vitro activity of a veterinary quinolone has to be evaluated on human strains isolated from the gut and especially against anaerobes. thus, the mics for marbofloxacin were determined by the reference agar method (norma nccls m11 a3) using wilkins chalgren medium for the 124 anaerobic strains and mueller hinton agar for e. coli, proteus, enterococcus and lactobacillus. on the whole aerobes, the activity of marbofloxacin was equal or slightly greater to that of of ...19968758470
direct evidence for a two-state protein unfolding transition from hydrogen-deuterium exchange, mass spectrometry, and nmr.we use mass spectrometry in conjunction with hydrogen-deuterium exchange and nmr to characterize the conformational dynamics of the 62-residue igg binding domain of protein l under conditions in which the native state is marginally stable. mass spectra of protein l after short incubations in d2o reveal the presence of two distinct populations containing different numbers of protected protons. nmr experiments indicate that protons in the hydrophobic core are protected in one population, whereas a ...19968762137
isolation of non-sporing anaerobic rods from infections in children.from 1974 to 1994, 2033 microbiological specimens from children were submitted for cultures for anaerobic bacteria. fifty-seven isolates of bifidobacterium spp. were obtained from 55 (3%) children, 67 isolates of eubacterium spp. from 65 (3%) children and 41 isolates of lactobacillus spp. from 40 (2%) children. most bifidobacterium isolates were from chronic otitis media, abscesses, peritonitis, aspiration pneumonia and paronychia. most eubacterium isolates were from abscesses, peritonitis, decu ...19968667406
bacteremia shortly after placental separation during cesarean delivery.to assess frequency, risk factors, and microbiology of bacteremia within 15 minutes of placental separation during cesarean delivery.19968677085
[computed tomographic demonstration of intraspinal air after an infection with peptostreptococci]. 19968679984
[diagnosis and treatment of local forms of surgical anaerobic non-clostridial infection].145 patients with such kind of surgical infection have been taken to clarify the cause of it. aerobic agents were the cause in 16.6% and anaerobic nonclostridial agents--in 33.1% of all cases. the mixed flora was the cause in 44.1%. the most important place among anaerobic nonclostridial infections belongs to bacteroids (38.3%), peptostreptococcus (27.3%) and the peptococcus (17.2%). one must use wide-spectrum antibiotic for a treatment of a such kind of infection tiel the usults of microbiologi ...19968683929
fetal fibronectin and microorganisms in vaginal fluid of healthy pregnant women.to determine fetal fibronectin in vaginal fluid from healthy women during uncomplicated pregnancy and to investigate possible relationships to gestational age and the vaginal microflora.19968693926
[a case of the greater omentum abscess due to peptostreptococcus ssp].a case of the greater omentum abscess due to peptostreptococcus ssp. is reported. a 63-year-old male was admitted to heisei memorial hospital with a complaint of abdominal pain. an intraperitoneal mass measuring 6 cm in diameter was detected by ct and ultrasonogram. he was diagnosed as having an intraperitoneal abscess. after antibiotics therapy by cefotiam, laparotomy was performed and an abscess about 7 cm in diameter at the edge of the greater omentum was found. there was no evidence of perfo ...19968699102
microbiology of subperiosteal orbital abscess and associated maxillary sinusitis.aspirate of pus from eight subperiosteal orbital abscesses (spoas) and their corresponding infected sinuses were studied for aerobic and anaerobic bacteria. polymicrobial flora was found in all instances, and the number of isolates varied from two to five. anaerobes were recovered from all specimens. the predominant isolates were peptostreptococcus spp, prevotella spp, fusobacterium spp, staphylococcus aureus, and microaerophilic streptococci. concordance in the microbiological findings between ...19968699891
pyomyositis in children, caused by anaerobic bacteria.the author describes the microbiology and clinical features of six pyomyositis infections in children, which yielded anaerobic bacteria. anaerobic bacteria alone were recovered in four instances, and they are mixed with facultative bacteria in two. there were 15 bacterial isolates (13 anaerobic, 2 facultative). the bacteria were peptostreptococcus sp (5 isolates), bacteroides fragilis (3), clostridium sp (2), fuso-bacterium nucleatum (1), prevotella sp (1), bateroides sp (1), streptococcus pyoge ...19968708910
identification of interdomain sequences promoting the intronless evolution of a bacterial protein family.in the evolution of eukaryotic genes, introns are believed to have played a major role in increasing the probability of favorable duplication events, chance recombinations, and exon shuffling resulting in functional hybrid proteins. as a rule, prokaryotic genes lack introns, and the examples of prokaryotic introns described do not seem to have contributed to gene evolution by exon shuffling. still, certain protein families in modern bacteria evolve rapidly by recombination of genes, duplication ...19968710897
anaerobic septic bursitis: case report and review. 19968722967
in vitro activity of the tricyclic beta-lactam gv104326.gv104326 is a novel tricyclic beta-lactam (a trinem or, formerly, tribactam). the in vitro activity of gv104326 was compared with those of cefuroxime, cefixime, amoxicillin, amoxicillin-clavulanic acid, cefpirome, and ciprofloxacin. gv104326 had in vitro activity generally similar to that of cefixime against members of the family enterobacteriaceae (mic at which 90% of the isolates are inhibited [mic90], < or = 2 micrograms/ml), with cefuroxime and amoxicillin-clavulanic acid being 8- to 32-fold ...19968723475
antibacterial activity of quinupristin/dalfopristin. rationale for clinical use.most gram-positive organisms are highly susceptible to the streptogramin, quinupristin/dalfopristin (rp 59500; synercid). minimum inhibitory concentrations for 90% of isolates (mic90) were < or = 1 mg/l for staphylococcus aureus, s. epidermidis, s. haemolyticus, streptococcus pneumoniae, s. pyogenes and listeria monocytogenes. importantly, quinupristin/dalfopristin shows similar activity against methicillin-susceptible and -resistant strains of s. aureus, and streptococci with benzylpenicillin ( ...19968724814
response to periodontal therapy in patients with high or low levels of p. gingivalis, p. intermedia, p. nigrescens and b. forsythus.in a previous study, subjects receiving either adjunctive tetracycline or augmentin showed, on average, more attachment level gain 10 months post-therapy than subjects receiving either ibuprofen or a placebo, although some subjects in each treatment group showed loss of attachment post-therapy. since differences in treatment response might have been due to differences in the subgingival microbiota, the response to different therapies in subjects with different pre-therapy subgingival microbiotas ...19968739165
utility of 5 major putative periodontal pathogens and selected clinical parameters to predict periodontal breakdown in patients on maintenance care.the predictive utility of 5 major putative periodontopathic microbial species, "superinfecting" organisms, and several clinical periodontal parameters were assessed relative to periodontitis recurrence over a 12-month period in 78 treated adult patients participating in a 3-month maintenance care program. at baseline, pooled subgingival microbial samples were collected from each patient, and whole-mouth evaluations of probing depth, relative periodontal attachment level, furcation involvement, a ...19968739166
comparison of spiral gradient endpoint and agar dilution methods for susceptibility testing of anaerobic bacteria: a multilaboratory collaborative evaluation.a multilaboratory collaborative study was carried out to assess the utility of the spiral gradient endpoint (sge) method for the determination of the antimicrobial susceptibilities of anaerobes and to evaluate the equivalence of the mics obtained by the sge method with those obtained by the reference agar dilution method of the national committee for clinical laboratory standards. the standard deviation of the mic obtained by the sge method for the five participating laboratories was +/- 0.26 of ...19968748295
backbone dynamics of a domain of protein l which binds to immunoglobulin light chains.protein l is a multidomain protein expressed at the surface of some strains of the anaerobic bacterial species peptostreptococcus magnus. the molecule interacts with the variable domain of immunoglobulin (ig) light chains through five repeated homologous domains denoted b1 to b5. the fold of the ig-light-chain-binding b1 domain of protein l (plb1) has been shown to comprise an alpha-helix packed against a four-stranded beta-sheet and therefore resembles the structure of the igg-binding domains o ...19968654399
effect of co2 on the fermentation capacities of the acetogen peptostreptococcus productus u-1.the fermentative capacities of the acetogenic bacterium peptostreptococcus productus u-1 (atcc 35244) were examined. although acetate was formed from all the substrates tested, additional products were produced in response to co2 limitation. under co2-limited conditions, fructose-dependent growth yielded high levels of lactate as a reduced end product; lactate was also produced under co2-enriched conditions when fructose concentrations were elevated. in the absence of supplemental co2, xylose-de ...19968655492
clinical significance of dental root canal microflora.previous work by this group has shown that a significant association exists between pain and the presence of either prevotella or peptostreptococcus spp. in dental root canals. the aim of this study was to examine a more extensive series of canals microbiologically, to determine whether any other particular endodontic symptoms or clinical signs showed specific associations with individual bacterial species.19968636492
aerobic and anaerobic microbiology of infected hemorrhoids.to study the aerobic and anaerobic microbiology of infected hemorrhoids.19968607502
aerobic and anaerobic microbiology of superficial suppurative thrombophlebitis.to study the aerobic and anaerobic microbiologic characteristics of superficial suppurative thrombophlebitis.19968546586
sodium-dependent succinate decarboxylation by a new anaerobic bacterium belonging to the genus peptostreptococcus.an anaerobic bacterium was isolated from a polluted sediment, with succinate and yeast extract as carbon and energy sources. the new strain was gram-positive, the cells were coccal shaped, the mol% g+g content of the genomic dna was 29, and the peptidoglycan was of the l-ornithine-d-glutamic acid type. comparative sequence analysis of the 16s rrna gene showed the new strain to belong to the genus peptostreptococcus. succinate, fumarate, pyruvate, 3-hydroxybutyrate and lysine supported growth. su ...19968836437
aerobic and anaerobic microbiology of necrotizing fasciitis in children.specimens obtained from eight children with necrotizing fasciitis (nf) were cultured for aerobic and anaerobic bacteria. a total of 21 isolates were recovered, 13 anaerobic and 8 aerobic or facultatives. the facultative organism streptococcus pyogenes was present alone in two (25%) instances, and mixed aerobic and anaerobic bacteria were isolated in six (75%). the predominant isolates were peptostreptococcus spp. (6 isolates, including 3 peptostreptococcus magnus). s. pyogenes (4), bacteroides f ...19968844744
infection of a total knee joint prosthesis by peptostreptococcus micros and propionibacterium acnes in an elderly ra patient: implant salvage with longterm antibiotics and needle aspiration/irrigation.we describe the favourable long-term outcome after late total knee joint prosthesis infection in an elderly ra patient. peptostreptococcus micros and propionibacterium acnes were cultured from the synovial fluid, and the finding of a coexistent abscess at the root of a wisdom tooth suggested a dental origin to the joint infection. long-term antibiotics in conjunction with aspiration/irrigation resulted in salvage of the implant and an excellent functional outcome.19968853177
characterization of the binding properties of protein lg, an immunoglobulin-binding hybrid protein.protein lg is a 50-kda hybrid molecule containing four ig-light-chain-binding domains from protein l of peptostreptococcus magnus and two igg-fe-binding repeats from streptococcal protein g. here we analyse the binding of protein lg to ig from several mammalian species. protein lg was shown to bind human igg of all subclasses and other ig classes that carry kappa chains. the binding to human igg was only marginally influenced by changes in temperature (4-37 degrees c) or salt concentration (0-1. ...19968856054
acute phase response in heifers with experimentally induced mastitis.ten pregnant heifers were inoculated in both hind udder quarters with a mixture of actinomyces pyogenes, fusobacterium necrophorum and peptostreptococcus indolicus. development of the experimental mastitis was monitored by sequential clinical and bacteriological examinations, and by blood acute phase protein profiles. sequential changes in plasma fibrinogen, serum haptoglobin, acid-soluble glyco-proteins and alpha 1-proteinase inhibitor activity were analysed and compared with both the clinical ...19968864931
deep neck infections. 19968870932
in vitro activities of trovafloxacin against 557 strains of anaerobic bacteria.the antimicrobial activity of trovafloxacin for 557 strains of anaerobic bacteria was determined by the national committee for clinical laboratory standards-approved wadsworth agar dilution technique. the species tested included bacteroides fragilis (n = 91), other members of the b. fragilis group (n = 130), campylobacter gracilis (n = 15), other bacteroides spp. (n = 16), prevotella spp. (n = 49), porphyromonas spp. (n = 15), fusobacterium spp. (n = 62), bilophila wadsworthia (n = 24), sutterel ...19968878617
protein pab, an albumin-binding bacterial surface protein promoting growth and virulence.the anaerobic bacterium peptostreptococcus magnus is a human commensal and pathogen. previous work has shown that strains of p. magnus isolated from patients with gynecological disease (vaginosis) frequently express an immunoglobulin (ig) light chain-binding protein called protein l. here we report that strains isolated from localized suppurative infections bind human serum albumin (hsa), whereas commensal isolates bind neither ig nor hsa. the hsa-binding protein pab was extracted from the bacte ...19968900134
in vitro activity of quinupristin/dalfopristin (rp 59500) against a large collection of infrequently isolated or tested species.quinupristin/dalfopristin (rp 59500, synercid) is a parenteral streptogramin combination antimicrobial that possesses a synergistic and often bactericidal action against many grampositive species. in this study, a collection of 1270 uncommonly isolated or tested strains were evaluated for susceptibility to quinupristin/dalfopristin using agar dilution minimum inhibitory concentration (mic) methods described in the national committe for clinical laboratory standards. the greatest antimicrobial ac ...19968902412
molecular analysis of microflora associated with dentoalveolar abscesses.the microflora associated with three dentoalveolar abscesses was determined by cultural and molecular methods. 16s rrna genes were randomly amplified by means of conserved eubacterial primers and cloned. restriction fragment length polymorphism analysis of the clones and amplified genes encoding 16s rrna from the cultured bacteria was used to detect putative unculturable bacteria. clones representative of five predominant groups of uncultured organisms were sequenced. two were identified as porp ...19968904410
[antimicrobial activity of cefodizime against clinical isolates].in order to evaluate antimicrobial activity of cefodizime (cdzm), minimum inhibitory concentrations (mics) of cdzm and control drugs were determined against clinical isolates collected from nation-wide medical institutions and in our laboratory from september to december of 1992 and from september to december of 1995. the results are summarized as follows: 1. bacterial species with no or few strains resistant to cdzm included streptococcus pyogenes, haemophilus influenzae, citrobacter koseri, pr ...19968986558
electrophoresis of whole-cell soluble proteins of microorganisms isolated from bacteremias in endodontic therapy.we have previously demonstrated that anaerobic bacteria are the microorganisms most frequently isolated from blood following endodontic therapy of teeth with apical periodontitis. phenotypic characterisation of the isolates suggested that the bacteria in the blood originated from the root canal. the present experiment using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) was carried out in an effort to verify these findings, and to further study the microorganisms involved i ...19969021323
development and evaluation of a selective and differential medium for the primary isolation of peptostreptococcus micros.peptostreptococcus micros, an anaerobic gram-positive coccus, has been associated with periodontal and endodontic lesions, including those refractory to treatment, as well as many human polymicrobial infections in other body locations. a selective and differential medium for the primary isolation of p. micros was developed and evaluated. columbia cna agar, a selective medium for gram-positive cocci, was supplemented with glutathione and lead acetate (p. micros medium: pmm). p. micros has a chara ...19969028263
vaginal microflora associated with bacterial vaginosis in japanese and thai pregnant women.the vaginal flora of 118 japanese and 208 thai pregnant women were investigated for the presence of bacterial vaginosis (bv), bv-associated organisms, and bv-associated enzyme. a similar prevalence of bv was found among the japanese (13.6%) and thai women (15.9%). the microbial flora of women with bv were complex; the mean number of isolates recovered in the bv group was approximately 2 times more than that in a group of healthy women. prevotella species, porphyromonas species, peptostreptococcu ...19968909838
microbiology of infected atopic dermatitis.bacterial infections occur frequently in lesions of atopic dermatitis (ad). the objectives of the study were to establish the aerobic and anaerobic microbiology of secondarily infected ad.19968915731
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