Publications

characterization of d: -lactate dehydrogenase from pediococcus acidilactici that converts phenylpyruvic acid into phenyllactic acid.	the gene coding for d: -lactate dehydrogenase (d: -ldh) from pediococcus acidilactici dsm 20284 was cloned and expressed in e. coli. the recombinant enzyme was purified by nickel-affinity chromatography. it converted phenylpyruvic acid (ppa) to 3-phenyllactic acid maximally at 30°c and ph 5.5 with a specific activity of 140 and 422 u/mg for ppa and pyruvate, respectively. the k (m), turnover number (k (cat)), and catalytic efficiency (k ...	2012	22261863
biodiversity of yeasts, lactic acid bacteria and acetic acid bacteria in the fermentation of "shanxi aged vinegar", a traditional chinese vinegar.	shanxi aged vinegar is a famous traditional chinese vinegar made from several kinds of cereal by spontaneous solid-state fermentation techniques. in order to get a comprehensive understanding of culturable microorganism's diversity present in its fermentation, the indigenous microorganisms including 47 yeast isolates, 28 lactic acid bacteria isolates and 58 acetic acid bacteria isolates were recovered in different fermenting time and characterized based on a combination of phenotypic and genotyp ...	2012	22265314
lactic acid bacteria from raw milk as potentially beneficial strains to prevent bovine mastitis.	bovine mastitis produces a wide variety of problems in the dairy farm. the treatment of this disease is based on the use of antibiotics which are not always effective. these drugs are also responsible for the presence of residues in the milk and the increase of antibiotic-resistant strains. probiotic products were proposed as a valid alternative to antibiotic therapies and are also useful for the prevention of infectious syndromes. with the aim of designing a probiotic product to prevent bovine ...	2012	22261519
Incorporating a mucosal environment in a dynamic gut model results in a more representative colonization by lactobacilli.	To avoid detrimental interactions with intestinal microbes, the human epithelium is covered with a protective mucus layer that traps host defence molecules. Microbial properties such as adhesion to mucus further result in a unique mucosal microbiota with a great potential to interact with the host. As mucosal microbes are difficult to study in vivo, we incorporated mucin-covered microcosms in a dynamic in vitro gut model, the simulator of the human intestinal microbial ecosystem (SHIME). We asse ...	2012	21989255
cow teat skin, a potential source of diverse microbial populations for cheese production.	the diversity of the microbial community on cow teat skin was evaluated using a culture-dependent method based on the use of different dairy-specific media, followed by the identification of isolates by 16s rrna gene sequencing. this was combined with a direct molecular approach by cloning and 16s rrna gene sequencing. this study highlighted the large diversity of the bacterial community that may be found on teat skin, where 79.8% of clones corresponded to various unidentified species as well as ...	2012	22081572