Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| lead toxicity and iron deficiency in utah migrant children. | we determined the frequency of presumptive iron deficiency and lead toxicity in 198 utah migrant children, ages 9-72 months, during the summer of 1985. there were no confirmed cases of lead toxicity. thirteen per cent of those tested and 30 per cent of the children ages 9-23 months were iron deficient. hematocrit determinations accurately predicted iron deficiency in only 35 per cent of the children confirmed to have this disorder via erythrocyte protoporphyrin screening. | 2009 | 2650572 |
| non-synaptic release from dense-cored vesicles occurs at all terminal types in crayfish neuropile. | in the crayfish neuropile, dense-cored vesicles (dcv) have been found in chemical terminals, mixed in with round or pleomorphic agranular synaptic vesicles, as well as in electrical terminals and neurohemal endings. dcv release their content at unspecialized non-synaptic sites. the simultaneous exocytosis of dcv and synaptic vesicles seems to be the rule in chemical terminals. dcv in specific terminals suggest non-synaptic communication. in chemical and electrical terminals, the content of dcv c ... | 2009 | 2702493 |
| [the rate of formation of necrotic focus and the severity of clinical picture in the acute period of myocardial infarction]. | the relationship between necrotic focus characteristics (weight and rate of necrotization) and the clinical pattern of myocardial infarction was examined. necrosis weight was shown to have no significant effect on the course of myocardial infarction, while maximum necrotization rate was found to be a valuable predictive factor: the higher the necrotization rate, the higher the risk of fatal outcome and various complications. prognosis, based on necrotization rate and patient's age, can be made, ... | 2009 | 2770081 |
| immune thrombocytopenia in aids patients: case report. | 2009 | 2795303 | |
| high potential of adhesion to abiotic and biotic materials in fish aquaculture facility by vibrio alginolyticus strains. | the ability of vibrio alginolyticus strains isolated from sparus aurata and dicentrarchus labrax nursery to adhere to epithelial cell lines (hep-2 and caco-2), fish mucus and their ability to form a biofilm on different surfaces (glass, polystyrene, polyethylene and polyvinyl-chloride) was investigated in this study. | 2009 | 19245411 |
| the effect of long-range hydrodynamic interaction on the swimming of a single bacterium. | it has been theoretically suggested that when a bacterium swims in a fluid, the disturbance it creates is long-ranged and can influence its locomotion. the contribution of these long-range hydrodynamic interactions to swimming cells is examined herein for a number of bacterial strains with well-defined flagellar geometries. we show experimentally for the first time that long-range hydrodynamic interactions are important for an accurate description of the swimming of a single cell, and the effect ... | 2009 | 19254562 |
| molecular cloning, characterization and expression analysis of a clip-domain serine protease from pearl oyster pinctada fucata. | the clip-domain serine proteases (sps) are the essential components of extracellular signaling cascade in various biological processes, especially in embryonic development and the innate immune responses of invertebrate. herein, we described the isolation and characterization of pearl oyster pinctada fucata clip-domain sp gene (designated as posp). the posp cdna was 1080 bp long and consisted of a 5'-untranslated region (utr) of 13 bp, a 3'-utr of 68 bp with a polyadenylation signal (aataaa) at ... | 2009 | 19254766 |
| purification, crystallization and preliminary x-ray analysis of an aminoacylhistidine dipeptidase (pepd) from vibrio alginolyticus. | the aminoacylhistidine dipeptidase (pepd) protein encoded by vibrio alginolyticus pepd was successfully overexpressed and characterized and the putative active-site residues responsible for metal binding and catalysis were identified. the purified enzyme contained two zinc ions per monomer. the recombinant dipeptidase enzyme, which was identified as a homodimer in solution, exhibited broad substrate specificity for xaa-his dipeptides, with highest activity towards the his-his dipeptide. the puri ... | 2009 | 19255468 |
| induction of phenoloxidases in the humoral fluids of amphioxus branchiostoma belcheri by vibrio alginolyticus and escherichia coli. | the humoral immune responses of amphioxus branchiostoma belcheri to microbial challenge remain open to date. here we examined the changes in po activity in the humoral fluids in amphioxus before and after challenge with escherichia coli and vibrio alginolyticus. it was found that po activity in the humoral fluids is markedly increased by challenge with e. coli and v. alginolyticus; and the microbial challenge results in a significant rise in subunit 2 of the three po subunits, making po subunit ... | 2009 | 19264135 |
| genomic diversity of vibrios associated with the brazilian coral mussismilia hispida and its sympatric zoanthids (palythoa caribaeorum, palythoa variabilis and zoanthus solanderi). | a taxonomic survey of the vibrios associated with the brazilian endemic coral mussismilia hispida and the sympatric zoanthids (i.e. palythoa caribaeorum, palythoa variabilis and zoanthus solanderi). | 2009 | 19291243 |
| [establishment of loop-mediated isothermal amplification for detection of vibrio alginolyticus]. | vibrio alginolyticus is the main vibrio pathogen in aquaculture in the south of china. a one step loop-mediated isothermal amplification (lamp) assay was developed for detection of vibrio alginolyticus. a set of primers were designed from the ompk sequence of vibrio alginolyticus. the assay was optimised to amplify vibrio alginolyticus dna by incubation at 65 degrees c for only 1 h, and required only a simple water bath or heating block to provide a constant temperature of 65 degrees c. lamp amp ... | 2009 | 19306691 |
| white shrimp litopenaeus vannamei that received the hot-water extract of gracilaria tenuistipitata showed earlier recovery in immunity after a vibrio alginolyticus injection. | white shrimp litopenaeus vannamei which had been immersed in seawater containing the hot-water extract of gracilaria tenuistipitata at 0 (control), 200, 400, and 600 mg l(-1) for 3 h, were challenged with vibrio alginolyticus at 4.6 x 10(6) colony-forming units (cfu) shrimp(-1) and then placed in normal seawater (34 per thousand). the survival rates of shrimp immersed in 200, 400, and 600 mg l(-1) of the hot-water extract were significantly higher than those of control shrimp over 48-120 h. in a ... | 2009 | 19332131 |
| effects of temperature change on the innate cellular and humoral immune responses of orange-spotted grouper epinephelus coioides and its susceptibility to vibrio alginolyticus. | orange-spotted grouper epinephelus coioides held at 27 degrees c were then further cultured at 19, 27 (control), and 35 degrees c, and were examined for innate cellular and humoral responses after 3-96 h. the total leucocyte count, respiratory burst, and phagocytic activity significantly decreased 3, 48, and 96 h after fish were transferred to 19 and 35 degrees c. both the alternative complement pathway (ach(50)) and the lysozyme activity significantly decreased at 3-96 h after fish were transfe ... | 2009 | 19332138 |
| identification of immune cells interacting with vibrio spp. and its in vitro post-phagocytic killing mechanism of haemocytes in the penaeid shrimp, penaeus indicus h. milne edwards. | immune cells were identified and their interaction towards vibrio alginolyticus, v. parahaemolyticus and v. anguillarum was studied in vitro in the penaeid shrimp, penaeus indicus. haemocytes were divided into agranulocytes, semi-dense granulocytes and dense granulocytes according to their morphology. agranulocytes (100%) and 0.3-0.7% of granulocytes were actively involved in coagulation. granulocytes were involved in in vitro phagocytosis and encapsulation of foreign materials. phagocytosis was ... | 2009 | 19335612 |
| identification and cloning of the alpha2-macroglobulin of giant freshwater prawn macrobrachium rosenbergii and its expression in relation with the molt stage and bacteria injection. | the full-length complementary (c)dna of the alpha2-macroglobulin (alpha2m) gene was cloned from haemocytes of the giant freshwater prawn macrobrachium rosenbergii by reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna end (race) methods. the 4875-bp cdna contains an open reading frame (orf) of 4419 bp, a 95-bp 5'-untranslated region (utr), and a 361-bp 3' utr containing the poly a tail. the orf encodes a protein of 1472 amino acids (aa) with a 23-residue sign ... | 2009 | 19340942 |
| suppressor of cytokine signaling 2 (socs-2) homologue in disk abalone: cloning, sequence characterization and expression analysis. | suppressor of cytokine signaling (socs) proteins are inhibitors of cytokine signaling pathways and key physiological regulators of both innate and adaptive immune systems in vertebrates. in present study, we provided the initial characterization of socs-2 homologue from the mollusk abalone haliotis discus discus as a member of invertebrate socs-2. the disk abalone socs-2 cdna (absocs-2) contains 1700-bp full length with 771-bp coding sequence, which codes 257 amino acids protein. based on the se ... | 2009 | 19340953 |
| isolation and characterization of a hepcidin peptide from the head kidney of large yellow croaker, pseudosciaena crocea. | large yellow croaker (pseudosciaena crocea) is one of the most important marine cultured fish in china. acidic extracts of five tissues of large yellow croaker showed strong anti-vibrio alginolyticus activity. acidic extract of head kidney tissue was subjected to heat-treatment in boiling water, and solid-phase extraction on sep-pak c(18) cartridge. it was found that the antibacterial substances were heat stable, and 20% acetonitrile effluent exhibited strong antibacterial activity. active extra ... | 2009 | 19344770 |
| enzymatic, outer membrane proteins and plasmid alterations of starved vibrio parahaemolyticus and vibrio alginolyticus cells in seawater. | the marine bacteria vibrio parahaemolyticus and v. alginolyticus were incubated in seawater for 8 months to evaluate their adaptative responses to starvation. the starved cells showed an altered biochemical and enzymatic profiles, respectively, on api 20e and api zym systems and an evolution to the filterable minicells state capable to pass membrane pore size 0.45 microm. outer membrane proteins patterns of stressed bacteria were also altered. indeed, these modifications were manifested by the a ... | 2009 | 19373459 |
| global climate change and wound care: case study of an off-season vibrio alginolyticus infection in a healthy man. | vibrio alginolyticus is a halophilic gram-negative bacterium normally present in seawater. vibrios are not capable of cutaneous invasion through intact skin and their isolation from extraintestinal sites is uncommon. however, interruptions in skin integrity (cuts or abrasions) can allow these bacteria to cause complicated skin and soft tissues infections. this case study describes the clinical assessment and management of a nonhealing traumatic wound, sustained in a coastal area during the winte ... | 2009 | 19387097 |
| acute effects of the antibiotic oxytetracycline on the bacterial community of the grass shrimp, palaemonetes pugio. | the toxicity of oxytetracycline (otc) was evaluated in adult grass shrimp, palaemonetes pugio. initially, static acute (96 h) toxicity tests were conducted with shrimp exposed from 0 to 1,000 mg/l otc. a calculated lethal concentration 50% value of 683.30 mg/l otc (95% confidence interval 610.85-764.40 mg/l) was determined from these tests, along with a lowest-observable-effect concentration of 750 mg/l and no-observable-effect concentration of 500 mg/l. moreover, chronic sublethal effects of ot ... | 2009 | 19400597 |
| phenotypic and genetic differences between opaque and translucent colonies of vibrio alginolyticus. | many pathogens undergo phase variation between rugose and smooth colony morphology or between opaque and translucent colony morphology, which is mainly due to the variation in the surface polysaccharides. in this study, vibrio alginolyticus zj-51 displayed phase variation between opaque, rugose colonies (op) and translucent, smooth colonies (tr). unlike the vibrios reported previously, tr cells of zj-51 enhanced biofilm formation and motility, but they did not differ from op cells in the quantit ... | 2009 | 19408137 |
| inhibitory effects of partially decomposed alginate on production of glucan and organic acid by streptococcus sobrinus 6715. | our previous study has already clarified that partially decomposed alginate (alg53) by vibrio alginolyticus sun53 has a competitive inhibitory effect on sucrase. the objective of this study is to evaluate the influence of alg53 on the production of glucan from sucrose by glucosyltransferase and acid from glucose by streptococcus sobrinus 6715. glucosyltransferase was prepared from cultural medium of s. sobrinus using ultrafiltration and hydroxyapatite chromatography. in order to examine the inhi ... | 2009 | 19430617 |
| inhibitor of rel/nf-kappab is regulated in sydney rock oysters in response to specific double-stranded rna and vibrio alginolyticus, but the major immune anti-oxidants ecsod and prx6 are non-inducible. | invertebrates, such as oysters have mechanisms to recognize different microbial associated molecular patterns (mamps) and recognition of these antigens triggers signaling pathways for the transcription of immune-related proteins. components of the rel/nf-(capital ka, cyrillic)b pathway have been identified in mollusks but the role of different mamps in the activation of this pathway is poorly understood. in the current study, the sydney rock oyster (saccostrea glomerata) was injected intramuscul ... | 2009 | 19464374 |
| role of alkaline serine protease, asp, in vibrio alginolyticus virulence and regulation of its expression by luxo-luxr regulatory system. | the alkaline serine protease asp, which was shown to be a virulence factor of vibrio alginolyticus as a purified protein, was cloned from v. alginolyticus epgs, a strain recently isolated from moribund epinephelus coioides in an outbreak of vibriosis in a mariculture farm of shenzhen. the asp null mutant was constructed by homologous recombination with suicide plasmid pnq705-1. compared with the wild-type strain, the asp null mutant exhibited a significant decrease of total extracellular proteas ... | 2009 | 19494689 |
| isolation of a novel strain of bacillus pumilus from penaeid shrimp that is inhibitory against marine pathogens. | a bacterium was isolated from the mid-gut of healthy black tiger shrimp, penaeus monodon, based on a large zone of inhibition in mixed culture on solid medium. the isolate was a gram-positive, motile spore former, with an optimum ph range for growth in tryptone soya broth containing 2% nacl of between ph 6 and 9. the bacterium was highly salt tolerant with concentrations between 0% and 8% having no detrimental effect on growth. the isolate was identified as bacillus pumilus based on physiologica ... | 2009 | 19573134 |
| a novel fas ligand in mollusk abalone: molecular characterization, immune responses and biological activity of the recombinant protein. | fas ligand is a member of the tnf superfamily that plays an important role by inducing apoptosis and homeostasis of immune responses. the gene encoding fas ligand was isolated from a disk abalone (haliotis discus discus) cdna library, denoted as the abfas ligand. it contains an 1832bp transcript with a 945bp open reading frame, encoding 315 amino acids. the abfas ligand showed characteristic transmembrane and tnf family signature domains. the deduced amino acid comparison showed that the abfas l ... | 2009 | 19576285 |
| mutational analysis of the gtp-binding motif of flhf which regulates the number and placement of the polar flagellum in vibrio alginolyticus. | precise regulation of the number and placement of flagella is critical for the mono-flagellated bacterium vibrio alginolyticus to swim efficiently. we previously proposed a model in which the putative gtpase flhf determines the polar location and generation of the flagellum, the putative atpase flhg interacts with flhf to prevent flhf from localizing to the pole, and thus flhg negatively regulates the flagellar number in v. alginolyticus cells. to investigate the role of the gtp-binding motif of ... | 2009 | 19605463 |
| identification of broad cross-protective immunogens using heterogeneous antiserum-based immunoproteomic approach. | bacterium is still one of the major causes of life-threatening microbial infections. the most effective way to control bacterial infections is probably vaccine prevention. however, development of bacterial vaccine, especially polyvalent vaccines that could be used to fight against a variety of bacterial serotypes and species, is challenging due to the difficulty in identifying broad cross-protective antigens for different serotypes and species of pathogenic bacteria. in the present study, we dev ... | 2009 | 19640004 |
| endophthalmitis caused by vibrio alginolyticus. | vibrio alginolyticus is a facultative anaerobic gram-negative bacillus found in normal marine flora. ocular infections induced by v. alginolyticus are extremely rare. we report a case of endophthalmitis caused by v. alginolyticus to draw attention to v. alginolyticus infections following ocular injuries. | 2009 | 19710275 |
| cloning and expression of a hepcidin gene from a marine fish (pseudosciaena crocea) and the antimicrobial activity of its synthetic peptide. | hepcidin gene is widely expressed in various fish, suggesting that this antimicrobial peptide is a very important component in the innate immune system. large yellow croaker (pseudosciaena crocea) is one of the important economic species of marine-cultured fish but knowledge of its innate immune mechanism is lacking. in this study, we characterize a p. crocea hepcidin gene named as pc-hepc. it consists of an open reading frame of 258 bases encoding 85 amino acids and has a conserved sequence in ... | 2009 | 19150638 |
| sodium-dependent dynamic assembly of membrane complexes in sodium-driven flagellar motors. | the bacterial flagellar motor is driven by the electrochemical potential of specific ions, h(+) or na(+). the motor consists of a rotor and stator, and their interaction generates rotation. the stator, which is composed of poma and pomb in the na(+) motor of vibrio alginolyticus, is thought to be a torque generator converting the energy of ion flux into mechanical power. we found that specific mutations in pomb, including d24n, f33c and s248f, which caused motility defects, affected the assembly ... | 2009 | 19183284 |
| molecular typing and identification of bdellovibrio-and-like organisms isolated from seawater shrimp ponds and adjacent coastal waters. | to apply and compare two pcr-based methods for typing saltwater bdellovibrio-and-like organisms (balos) and to understand ecological and phylogenetic aspects of the balos isolated from shrimp mariculture systems. | 2009 | 19191952 |
| depuration of striped venus clam (chamelea gallina l.): effects on microorganisms, sand content, and mortality. | this study was focused on the evaluation of the microbiological indices, defined by european legislation, before and after the depuration of clams (chamelea gallina) landed in category b seawater. the survival of depurated clams and the meat yield were also evaluated. the results obtained from october 2002 to september 2003 evidenced a mean microbial reduction during depuration of 62% for escherichia coli and 54% for fecal coliforms (fc). all the samples had fc counts below the limit after 24 h ... | 2009 | 19200100 |
| alterations of outer membrane proteins and virulence genes expression in gamma-irradiated vibrio parahaemolyticus and vibrio alginolyticus. | gamma-irradiation technology sterilizes microorganisms and thereby prevents decay and improves the safety and shelf stability of food products. in this study we treated the foodborne pathogens vibrio parahaemolyticus and vibrio alginolyticus with gamma-irradiation (0.5 kgy) to evaluate their adaptative response. outer membrane protein patterns of irradiated bacteria were found altered when analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. these modifications were manifested ... | 2009 | 19735197 |
| conformational and dynamics simulation study of antimicrobial peptide hedistin-heterogeneity of its helix-turn-helix motif. | hedistin is an antimicrobial peptide isolated from the coelomocytes of nereis diversicolor, possessing activity against a large spectrum of bacteria including the methicillin resistant staphylococcus aureus and vibrio alginolyticus. the three-dimensional structure of hedistin in both aqueous solution and deuterated dodecylphosphocholine (dpc) micelles was examined using circular dichroism (cd) and nuclear magnetic resonance (nmr) techniques. and, the early events of the antibacterial process of ... | 2009 | 19819221 |
| interaction between na+ ion and carboxylates of the poma-pomb stator unit studied by atr-ftir spectroscopy. | bacterial flagellar motors are molecular machines powered by the electrochemical potential gradient of specific ions across the membrane. the poma-pomb stator complex of vibrio alginolyticus couples na(+) influx to torque generation in this supramolecular motor, but little is known about how na(+) associates with the poma-pomb complex in the energy conversion process. here, by means of attenuated total reflection fourier-transform infrared (atr-ftir) spectroscopy, we directly observed binding of ... | 2009 | 19894756 |
| investigation of several virulence properties among vibrio alginolyticus strains isolated from diseased cultured fish in tunisia. | we analysed 34 vibrio alginolyticus strains isolated from gilthead sea bream sparus aurata l. and sea bass dicentrarchus labrax l. cultured in fish farms on the tunisian mediterranean coast for the presence of several virulence properties such as extracellular products (ecp) production, growth in iron-limiting conditions and survival in fish serum. the results obtained with different substrates showed that ecp of v. alginolyticus were hydrolytic. the virulence was correlated with the ability of ... | 2009 | 19899346 |
| cloning, identification, and characterization of the rpos-like sigma factor rpox from vibrio alginolyticus. | vibrio alginolyticus zj-51 displays phase variation between opaque/rugose colonies (op) and translucent/smooth colonies (tr). these colony variants show great differences in biofilm formation and motility. in this study, a gene encoding for an rpos-like sigma factor, rpox, has been cloned and characterized. the absence of rpox did not affect colony switching rate but did decrease biofilm formation in both the op and the tr variants. when challenged with hydrogen peroxide, the deltarpox in the op ... | 2009 | 20069110 |
| the expression of protein disulfide isomerase from litopenaeus vannamei hemocytes is regulated by bacterial inoculation. | a partial clone encoding a member of the protein disulfide isomerase (pdi) was isolated from a litopenaeus vannamei hemocyte cdna library. the 5?-end sequence was obtained by race. the complete sequence encodes for a 502-residues protein that contains two thioredoxin domains and the typical endoplasmic reticulum retention kdel motif. shrimp pdi is highly similar to the homologue protein described in both vertebrates and invertebrates. changes in the shrimp pdi mrna expression were observed after ... | 2009 | 20403767 |
| [development of taqman real-time pcr in detection of aeromonas hydrophila]. | to develop a taqman real-time pcr for the detection of aeromonas hydrophila. | 2009 | 19954074 |
| the implication of yggt of escherichia coli in osmotic regulation. | an escherichia coli mutant lacking three major k(+) uptake systems, trk, kup, and kdp, did not grow under low k(+)and high na(+) concentrations. the introduction of fkua and of fkub of a marine bacterium, vibrio alginolyticus, has been reported to compensate for the growth defect by accelerating the rate of k(+) uptake (nakamura, katoh, shimizu, matsuba, and unemoto, biochim. biophys. acta, 1277, 201-208 (1996)). we investigated the function of unknown genes of e. coli, yggs and yggt, homologs o ... | 2009 | 19966467 |
| cdna cloning, identification, tissue localisation, and transcription profile of a transglutaminase from white shrimp, litopenaeus vannamei, after infection by vibrio alginolyticus. | complementary (c)dna encoding transglutaminase (tg) messenger (m)rna of white shrimp, litopenaeus vannamei, was cloned from haemocytes by a reverse-transcription polymerase chain reaction (rt-pcr) and rapid amplification of cdna ends (race) using oligonucleotide primers based on the tg sequence of the horseshoe crab, tachypleus tridentatus (accession no.: baa02134); tiger shrimp, penaeus monodon (aal78166); and pacifastacus leniusculus (af336805). the 2638-bp cdna contained an open reading frame ... | 2009 | 19782141 |
| dietary medicinal plant extracts improve growth, immune activity and survival of tilapia oreochromis mossambicus. | the effects of supplementing diets with acetone extract (1% w/w) from four medicinal plants (bermuda grass cynodon dactylon, h(1), beal aegle marmelos, h(2), winter cherry withania somnifera, h(3) and ginger zingiber officinale, h(4)) on growth, the non-specific immune response and ability to resist pathogen infection in tilapia oreochromis mossambicus were assessed. in addition, the antimicrobial properties of the extract were assessed against vibrio alginolyticus, vibrioparahaemolyticus, vibri ... | 2009 | 20735646 |
| first molluscan tnf-alpha homologue of the tnf superfamily in disk abalone: molecular characterization and expression analysis. | tumor necrosis factor alpha (tnf-alpha) is considered as a multifunctional immune modulator that plays an important role in the innate and adaptive immune systems in vertebrates. here, we described the characterization and expression analysis of the first tnf-alpha homologue in mollusk abalone, named as abtnf-alpha. it has 930-bp full length with a 717-bp open reading frame (orf), encoding 239 amino acids. the abtnf-alpha amino acid sequence shows the characteristic tnf family signature, n-termi ... | 2009 | 18984056 |
| a second propo present in white shrimp litopenaeus vannamei and expression of the propos during a vibrio alginolyticus injection, molt stage, and oral sodium alginate ingestion. | prophenoloxidase (propo) is a melanin-synthesising enzyme that plays important roles in immune responses by crustaceans. previously, we cloned and characterized propo-i from white shrimp, litopenaeus vannamei. in the present study, a novel prophenoloxidase-ii (propo-ii) cdna was also cloned from haemocytes of l. vannamei using oligonucleotide primers and reverse-transcriptase polymerase chain reaction (rt-pcr). both 3'- and 5'-regions were isolated by the rapid amplification of complementary (c) ... | 2009 | 18984057 |
| enhancement of immunity and disease resistance in the white shrimp, litopenaeus vannamei, by the probiotic, bacillus subtilis e20. | effects of bacillus subtilis e20 isolated from fermented soybean on immune parameters and the disease resistance of the white shrimp (litopenaeus vannamei) after 98 days of b. subtilis e20 feeding were evaluated in this study. shrimp fed b. subtilis e20-containing diets at concentrations of 10(6) (e206), 10(7) (e207), and 10(8) (e208)cfu kg(-1), respectively, had significantly increased survival rates of 13.3%, 16.7%, and 20%, compared to the control (fed no probiotic) after being challenged wit ... | 2009 | 19111620 |
| evaluation of the antibacterial activity of leaf and twig extracts of stout camphor tree, cinnamomum kanehirae, and the effects on immunity and disease resistance of white shrimp, litopenaeus vannamei. | effects of essential oils and hot-water extracts isolated from leaf and twig of stout camphor tree, cinnamomum kanehirae on antibacterial activity to pathogen of fish, abalone, marine fish and freshwater prawn, and the white shrimp, litopenaeus vannamei immunity and disease resistance to vibrio alginolyticus were carried out in this study. a better antibacterial activity against nine selected pathogen bacteria was recorded in twig essential oil, and the selected pathogens of both gram-positive b ... | 2009 | 19063975 |
| molecular characterization and expression analysis of the ikappab gene from pearl oyster pinctada fucata. | inhibitor of nf-kappab (ikappab) is one important member of nf-kappab signal pathway and plays a pivotal role in regulating the innate immune response of invertebrate. herein, we described the isolation and characterization of pearl oyster pinctada fucata ikappab gene (designated as poikappab). the poikappab cdna was 1975 bp long and consisted of a 5' untranslated region (utr) of 73 bp, a 3' utr of 807 bp with three rna instability motifs (attta) and a polyadenylation signal (aataaa) at 13 nucle ... | 2009 | 19010421 |
| cloning and expression of heat shock protein 70 gene in the haemocytes of pearl oyster (pinctada fucata, gould 1850) responding to bacterial challenge. | the cdna of pearl oyster pinctada fucata hsp70 (designated pfhsp70) was cloned by est and rapid amplification of cdna ends (race) techniques. the full length of pfhsp70 cdna was 2376 bp, consisting of a 5'-terminal untranslated region (utr) of 89 bp, a 3' terminal utr of 328 bp, and an open reading frame (orf) of 1959 bp encoding a polypeptide of 652 amino acids with a theoretical molecular weight of 71.42 kda and an estimated isoelectric point of 5.18. blast analysis revealed that the pfhsp70 g ... | 2009 | 19026750 |
| molecular cloning, characterization, expression and antibacterial analysis of a lysozyme homologue from fenneropenaeus merguiensis. | the gene coding for lysozyme in banana prawn (fenneropenaeus merguiensis) was cloned, sequenced and expressed in pet-32a vector. the deduced amino acid sequence of f. merguiensis lysozyme showed 37-93% similarity with the mouse, human, chicken, and tiger prawn counterparts. the lysozyme was purified to homogeneity and observed as a band of approximately 15 kda in 12% sds-page. semiquantitative rt-pcr analysis demonstrated that mrna transcripts of lysozyme could be mainly detected in the tissues ... | 2009 | 18798009 |
| immunoglobulin m gene expression analysis of orange-spotted grouper, epinephelus coioides, following heat shock and vibrio alginolyticus challenge. | the present study aimed to examine the expression of immunoglobulin m (igm) gene in orange-spotted grouper (epinephelus coioides) following thermal stress, bacterial infection, and immunization with formalin-killed vibrio alginolyticus, a kind of bacterial pathogen that causes septicemia. in heat shock experiments, twenty-five healthy orange-spotted grouper were kept in tanks with seawater at 37±0.5°c for one hour heat-shock treatment, and then returned to 27±0.5°c seawater tanks. in bacterial c ... | 2010 | 20816805 |
| f-type lectin involved in defense against bacterial infection in the pearl oyster (pinctada martensii). | in invertebrates and vertebrates, carbohydrate-binding proteins (lectins) play an important role in innate immunity against microbial invasion. in the present study, we report the cloning of an f-type lectin (designated as pmf-lectin) from pearl oyster (pinctada martensii) using a combination of expression sequence tag (est) analysis and rapid amplification of cdna ends (race) pcr. the full-length cdna of pmf-lectin contains an open reading frame (orf) of 579 bp coding for192 amino acids. the de ... | 2010 | 21195768 |
| immune parameters in the humoral fluids of amphioxus branchiostoma belcheri challenged with vibrio alginolyticus. | the knowledge concerning the humoral immunity is scarce in amphioxus branchiostoma belcheri. this study measured the humoral parameters including lysozyme, antimicrobial activity, microbial agglutinin and haemagglutinin in amphioxus humoral fluids before and after vibrio alginolyticus challenge. after challenged with v. alginolyticus, the lysozyme activity, growth inhibiting activities against escherichia coli and v. alginolyticus and microbial agglutinating activities against micrococcus lysode ... | 2010 | 19800008 |
| molecular cloning and characterization of a heat shock protein 70 gene in swimming crab (portunus trituberculatus). | hsp70 can stimulate cells of the innate immune system directly by acting as "danger"-signaling molecules. to understand the immune defense mechanisms of swimming crab portunus trituberculatus (decapoda: brachyura: portunidae), the cdna of hsp70 (designated pthsp70) was cloned by the combination of homology cloning and rapid amplification of cdna ends (race). the full-length pthsp70 cdna was 2195 bp, including an open reading frame (orf) of 1950 bp encoding a polypeptide of 650 amino acids with e ... | 2010 | 19815077 |
| the flagellar basal body-associated protein flgt is essential for a novel ring structure in the sodium-driven vibrio motor. | in vibrio alginolyticus, the flagellar motor can rotate at a remarkably high speed, ca. three to four times faster than the escherichia coli or salmonella motor. here, we found a vibrio-specific protein, flgt, in the purified flagellar basal body fraction. defects of flgt resulted in partial fla⁻ and mot⁻ phenotypes, suggesting that flgt is involved in formation of the flagellar structure and generating flagellar rotation. electron microscopic observation of the basal body of δflgt cells reveale ... | 2010 | 20729351 |
| first molluscan transcription factor activator protein-1 (ap-1) member from disk abalone and its expression profiling against immune challenge and tissue injury. | the regulation of transcriptional activation is an essential and critical point in gene expression. in this study, we describe a novel transcription factor activator protein-1 (ap-1) gene from disk abalone haliotis discus discus (abap-1) for the first time in mollusk. it was identified by homology screening of an abalone normalized cdna library. the cloned abap-1 consists of a 945 bp coding region that encodes a putative protein containing 315 amino acids. the abap-1 gene is composed of a charac ... | 2010 | 20732431 |
| expression of hsp60 and hsp70 in white shrimp, litopenaeus vannamei in response to bacterial challenge. | in the present study, cdna encoding a heat shock protein 60 (lvhsp60) gene in litopenaeus vannamei was cloned using a combination of homology and rapid amplification of cdna end (race) methods. the full length of the lvhsp60 cdna was found to be 2379bp, with a 1737bp open reading frame. the translated amino acid sequence consisted of 579 residues with a calculated molecular mass of 60.8kd and an isoelectronic point (pi) of 5.97. comparison of the deduced amino acid sequence showed that it has hi ... | 2010 | 20043915 |
| rapid identification and characterization of vibrio species using whole-cell maldi-tof mass spectrometry. | vibrio identification by means of traditional microbiological methods is time consuming because of the many biochemical tests that have to be performed to distinguish closely related species. this work aimed at evaluating the use of maldi-tof mass spectrometry for the rapid identification of vibrio (v.) spp. as an advantageous application to rapidly discriminate the most important vibrio spp. and distinguish vibrio spp. from closely related bacterial species like photobacterium damselae and grim ... | 2010 | 20059616 |
| disk abalone (haliotis discus discus) expresses a novel antistasin-like serine protease inhibitor: molecular cloning and immune response against bacterial infection. | a novel antistasin-like cdna homologue named as ab-antistasin was isolated from the disk abalone haliotis discus discus normalized cdna library. the ab-antistasin (1398-bp) consisted of an 1185-bp open reading frame encoding 395 amino acid (aa) residues. the predicted molecular mass and isoelectric point of ab-antistasin was 44 kda and 8.5, respectively, and showed highest identity (23.1%) to hydra magnipapillata antistasin. the most striking feature of ab-antistasin is the 12-fold internal repe ... | 2010 | 20060477 |
| cloning and characterization of two ferritin subunit genes from bay scallop, argopecten irradians (lamarck 1819). | we have cloned two full-length cdnas from two ferritin genes (aifer1 and aifer2) of the bay scallop, argopecten irradians (lamarck 1819). the cdnas are 1,019 and 827 bp in length and encode proteins of 171 and 173 amino acids, respectively. the 5' utr of each contains a conserved iron response element (ire) motif. sequence analyses reveal that both proteins belong to the h-ferritin family with seven conserved amino acids in the ferroxidase center. highest expression of aifer1 is found in the man ... | 2010 | 20878240 |
| diet enriched with mushroom phellinus linteus extract enhances the growth, innate immune response, and disease resistance of kelp grouper, epinephelus bruneus against vibriosis. | the effect of diet supplemented with phellinus linteus fed for 30 days was investigated in grouper epinephelus bruneus challenged with vibrio anguillarum, vibrio harveyi, vibrio alginolyticus, and vibrio carchariae; infected and treated fish had a significantly higher percent weight gain and feed efficiency. in groups fed with enriched diet and challenged with v. anguillarum and v. harveyi the mortality rate declined with a consequent rise in survival rate than with other pathogens. on the other ... | 2010 | 20883799 |
| an acute gastroenteritis outbreak of vibrio parahaemolyticus o4:k55 in nursing college, thailand. | a cluster of acute gastroenteritis among nursing students was noticed on 13th september 2005. between 13th and 17th september 2005, a retrospective cohort study was then conducted to identify the most likely cause of gastroenteritis at a nursing college in bangkok, thailand. self-administered questionnaires, interviews, environmental investigations, and rectal swabs from all participants were carried out. in the investigation, 98.9% female nursing students were investigated and had completed the ... | 2010 | 20962724 |
| transcriptional regulation of extracellular copper zinc superoxide dismutase from white shrimp litopenaeus vannamei following vibrio alginolyticus and wssv infection. | the cdna encoding an extracellular copper zinc superoxide dismutase (lvecsod) was cloned from the hepatopancreas of white shrimp litopenaeus vannamei. it consisted of 915 bp nucleotides with an open reading frame corresponding to a deduced protein of 178 amino acids. the lvecsod contains a putative signal peptide of 16 amino acids, two potential n-linked glycosylation sites (n(115)gta and n(135)itg) and a copper zinc superoxide dismutase family signature sequence (g(162)nagarvaccti(173)). it was ... | 2010 | 20974259 |
| identification of outer membrane proteins of vibrio parahaemolyticus and vibrio alginolyticus altered in response to γ-irradiation or long-term starvation. | vibrio parahaemolyticus and vibrio alginolyticus were subjected to γ-irradiation (0.5 kgy) or starvation by incubation for 8 months in seawater to study modifications in their outer membrane protein patterns. after treatment, outer membrane protein profiles of starved or γ-irradiated bacteria were found to be altered when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (sds-page). altered proteins were identified by mass spectrometry (ms and ms/ms) and analyses revealed tha ... | 2010 | 21035543 |
| autophagy is induced by the type iii secretion system of vibrio alginolyticus in several mammalian cell lines. | vibrio alginolyticus is a gram-negative bacterium and has been recognized as an opportunistic pathogen in marine animals as well as humans. here, we further characterized a cell death mechanism caused by this bacterium in several mammalian cell lines. the t3ss of v. alginolyticus killed hela cells by a very similar cell cytolysis mechanism in fish cells, as evidenced by cell rounding and ldh release; however, dna fragmentation was not observed. further studies showed that caspase-1 and caspase-3 ... | 2010 | 21046072 |
| galectin-1 (ofglec-1) from rock bream (oplegnathus fasciatus): identification, characterization, gene expression and functional properties. | lectins may function as pattern-recognition receptors (prrs) and play important roles in immune responses. in this study, a novel galectin-1 (designated ofglec-1) gene was identified from rock bream, oplegnathus fasciatus, cdna library. the complete cdna sequence of the ofglec-1 gene is 993 base pairs in length, and encodes 135 amino acids. ofglec-1 shares a highly conserved carbohydrate recognition domain with galectin-1 from other fishes. the mrna expressions of ofglec-1 in healthy and bacteri ... | 2010 | 21073891 |
| a polyphasic approach for the differentiation of environmental vibrio isolates from temperate waters. | climate change and marine traffic lead to changing species communities in the oceans. due to increasing seawater temperatures, pathogenic vibrio species could become significant even in temperate waters. we classified mesophilic vibrio isolates from the german bight (north sea) using a polyphasic approach with special emphasis on vibrio parahaemolyticus. matrix-assisted laser desorption/ionization time-of-flight ms was used as a primary screen to classify isolates, 16s rrna gene and rpob gene se ... | 2010 | 21118277 |
| molluscan death effector domain (ded)-containing caspase-8 gene from disk abalone (haliotis discus discus): molecular characterization and expression analysis. | the caspase family represents aspartate-specific cysteine proteases that play key roles in apoptosis and immune signaling. in this study, we cloned the first death effector domain (ded)-containing molluscan caspase-8 gene from disk abalone (haliotis discus discus), which is named as hdcaspase-8. the full-length hdcaspase was 2855 bp, with a 1908 bp open reading frame encoding 636 amino acids. the hdcaspase-8 had 72 kda predicted molecular mass with an estimated isoelectric point (pi) of 6.0. the ... | 2010 | 21130887 |
| development of a single base extension-tag microarray for the detection of pathogenic vibrio species in seafood. | in this study, a single base extension-tag array on glass slides (sbe-tags) microarray was established to detect the seven leading seafood-borne pathogens, including vibrio parahaemolyticus, vibrio cholerae, vibrio vulnificus, vibrio mimicus, vibrio alginolyticus, vibrio anguillarum, and vibrio harveyi. three multiplex pcr assays were developed to specifically target the following species with individual gene markers, which are aads, tdh, and trh for v. parahaemolyticus; col, toxr, and vvh for v ... | 2010 | 21161212 |
| two flagellar stators and their roles in motility and virulence in pseudomonas syringae pv. tabaci 6605. | the motor proteins around the flagellar basal body consist of two cytoplasmic membrane proteins, mota and motb, and function as a complex that acts as the stator to generate the torque that drives rotation. genome analysis of several pseudomonas syringae pathovars revealed that there are two sets of genes encoding motor proteins: motab and motcd. deduced amino acid sequences for mota/b and motc/d showed homologies to the h(+)-driven stator from escherichia coli and na(+)-driven stator from vibri ... | 2010 | 21165649 |
| identification of a luxo-regulated extracellular protein pep and its roles in motility in vibrio alginolyticus. | the fish pathogen vibrio alginolyticus virulence factors and regulation mechanism are barely known except the quorum sensing regulated extracellular products. in the present study, the cleavable isotope-coded affinity tags (cicats) were used to analyze the differentially expressed extracellular proteins of v. alginolyticus wild-type and a δluxo mutant, and a putative peptidase pep was identified. quantitative real-time reverse transcription pcr confirmed that the transcription of pep was down-re ... | 2010 | 21167274 |
| three isoforms of anti-lipopolysaccharide factor identified from eyestalk cdna library of swimming crab portunus trituberculatus. | anti-lipopolysaccharide factors (alfs), as the potent antimicrobial peptides, can bind and neutralize lipopolysaccharide (lps) and exhibit broad spectrum antimicrobial activities. in this study, three isoforms of the alf homologues (ptesalf1-3) were identified from eyestalk cdna library of swimming crab portunus trituberculatus. the full-length cdna sequences of ptesalf1, 2 and 3 were 1138, 1052 and 1057 bp encoding 92, 108 and 123 amino acids, respectively. ptesalf1-3 contained two conserved cy ... | 2010 | 21168510 |
| functional characterization of vibrio alginolyticus twin-arginine translocation system: its roles in biofilm formation, extracellular protease activity, and virulence towards fish. | the bacterial twin-arginine translocation (tat) system contributes to translocate folded proteins and plays pleiotropic roles in growth, motility, and the secretion of some virulent factors. in this study, the authors identified the tat gene cluster in fish pathogen vibrio alginolyticus and explored its roles in pathogenesis toward fish. vibrio alginolyticus tat mutants showed growth deficiency in tmao medium, while the complement strain restored the ability to grow in the medium, demonstrating ... | 2010 | 21170534 |
| [cloning and expression pattern analysis of a lipopolysaccharide -and beta-1,3-glucan-binding protein in portunus trituberculatus]. | the lipopolysaccharide -and beta-1,3-glucan-binding protein (lgbp) is a pattern recognition receptor, which is fundamental for the innate immune response of crustaceans. a lgbp gene was cloned from the haemocytes of portunus trituberculatus using smart race methods. the full-length lgbp cdna (1 378 bp) had a 1 095 bp open reading frame encoding a protein of 365 amino acid residues including a 16 amino acid residues signal peptide, a 138 bp 5' untranslated region (utr) and a 144 bp untranslated r ... | 2010 | 21174347 |
| differential expression of lvhsp60 in shrimp in response to environmental stress. | previous studies showed that heat-shock protein 60 (hsp60) was known to function as a molecular chaperone and is an important factor in the innate immune system in mammals. however, little was known about the physiological relevance of hsp60 in marine invertebrates. this study focuses on long-term monitoring of the differential expression of lvhsp60 in shrimp litopenaeus vannamei in response to environmental stress. the thermal aggregation assay elucidated that lvhsp60 was an effective chaperone ... | 2010 | 21182965 |
| phylogenetic and evolutionary analysis of vibrio parahaemolyticus and vibrio alginolyticus isolates based on toxr gene sequence. | the vibrio genus contains a large number of closely related bacterial species differing, in some cases, less than 1% in 16s rrna gene sequence. the present study evaluated the usefulness of toxr gene for phylogenetic and evolution analysis on vibrio isolates of environmental or clinical origin belonging to the two closely related species v. parahaemolyticus and v. alginolyticus. the phylogenetic analysis based on toxr gene, contrary to 16s rrna gene, allowed a clear differentiation of the isolat ... | 2010 | 21213595 |
| molecular cloning and characterization of a novel c-type lysozyme gene in swimming crab portunus trituberculatus. | lysozymes are key proteins to invertebrates in the innate immune responses against bacterial infections. in this study, the full-length cdna of a novel c-type lysozyme was cloned from the haemocytes of swimming crab portunus trituberculatus using rapid amplification of cdna ends (race) approaches. the full-length cdna of lysozyme was of 984 bp, containing a 5' untranslated region (utr) of 90 bp, a 3' utr of 222 bp with a poly (a) tail, and an open reading frame (orf) of 672 bp encoding an 18 ami ... | 2010 | 20420912 |
| the protective immunity of white shrimp litopenaeus vannamei that had been immersed in the hot-water extract of gracilaria tenuistipitata and subjected to combined stresses of vibrio alginolyticus injection and temperature change. | white shrimp litopenaeus vannamei which had been immersed in seawater (35 per thousand) containing the hot-water extract of gracilaria tenuistipitata at 0 (control), 400, and 600 mg l(-1) for 3 h, were subjected to temperature transfer (28 degrees c), or combined stresses of vibrio alginolyticus injection (2.4 x 10(6) colony-forming unit shrimp(-1)) and temperature transfer (28 degrees c) from 24 degrees c, and the immune parameters including hyaline cells (hcs), granular cells (gcs, including s ... | 2010 | 20420916 |
| expression, characterization and immunogenicity of flagellin flac from vibrio alginolyticus strain hy9901. | vibrio alginolyticus is one of ubiquitous pathogens infecting human and marine animals. flagellins of bacteria play an important role in infecting animals and inducing host immune response. in the present research, flagellin flac gene of v. alginolyticus strain hy9901 was cloned and expressed. the open reading frame of flac gene contains 1155 bp and the putative protein consists of 384 amino acid residues. polyclonal antibodies were raised in mouse against the purified recombinant flac protein a ... | 2010 | 20420917 |
| characterization and expression analysis of ef hand domain-containing calcium-regulatory gene from disk abalone: calcium homeostasis and its role in immunity. | the complete amino acid sequence of a calcium-regulatory gene (denoted as ab-careg i) was identified from the disk abalone haliotis discus discus cdna library. the ab-careg i is composed of 176 amino acids and the calculated molecular mass and isoelectric point were 20 and 4.2, respectively. the sequence homology of ab-careg i was 28-30 and 18-27% of known calmodulin and troponin c, respectively. four characteristic calcium-binding ef hand motifs with some modifications at conserved positions of ... | 2010 | 20420919 |
| new angucyclines and antimicrobial diketopiperazines from the marine mollusk-derived actinomycete saccharothrix espanaensis an 113. | two new angucyclines, saccharothrixmicines a (2) and b (3), together with three known diketopiperazines 4-6 were isolated from the actinomycete saccharothrix espanaensis an 113 associated with the marine mollusk anadara broughtoni. their structures were determined by hresi-ms and 1d and 2d nmr. compounds 2 and 3 differ in aglycone and glycosidic bond type. 2 is an alpha-l-6-deoxyaltrose-phenylglycoside of a benz[a]anthraquinone aglycone, while 3 is an o-glycoside of the same sugar linked to c-7 ... | 2010 | 20433079 |
| allograft inflammatory factor-1 in disk abalone (haliotis discus discus): molecular cloning, transcriptional regulation against immune challenge and tissue injury. | here, we report the identification and characterization of allograft inflammatory factor-1 (aif-1) from disk abalone haliotis discus discus that was denoted as abaif-1. the full-length cdna of abaif-1 consists of a coding region (453 bp) for 151 amino acids with a 17 kda molecular mass. analysis of abaif-1 sequence showed that it shares characteristic two ef hand ca(+2)-binding motifs. results from phylogenetic analysis further confirm that abaif-1 is a member of the aif-1 family similar to inve ... | 2010 | 20435145 |
| molecular characterization and expression analysis of interferon-gamma-inducible lysosomal thiol reductase (gilt) gene from pearl oyster pinctada fucata. | interferon-gamma-inducible lysosomal thiol reductase (gilt) is an important thiol reductase, involved in class, mhc-restricted antigen processing by catalyzing disulfide bond reduction in mammals. herein, we describe the identification and characterization of pearl oyster pinctada fucata gilt (designated as pogilt). the pogilt cdna was 1273bp long and consisted of a 5'-untranslated region (utr) of 24bp, a 3'-utr of 484bp with two cytokine rna instability motifs (attta), and an open reading frame ... | 2010 | 20444427 |
| identification of non-coding rnas in environmental vibrios. | the discovery of non-coding rna (ncrna) has been mainly limited to laboratory model systems and human pathogenic bacteria. in this study, we begin to explore the ncrna diversity in four recently sequenced environmental vibrio species (vibrio alginolyticus 40b, vibrio communis 1da3, vibrio mimicus vm573 and vibrio campbellii baa-1116) by performing in silico searches using infernal and rfam for the identification of putative ncrna-encoding genes. this search method resulted in the identification ... | 2010 | 20447992 |
| an in vitro and in vivo assessment of the potential of vibrio spp. as probiotics for the pacific white shrimp, litopenaeus vannamei. | the objective of the work was to determine whether known strains of nonpathogenic vibrios can act as probiotics for the control of vibrio infections in the pacific white shrimp, litopenaeus vannamei. | 2010 | 20477892 |
| cloning, expression, and genus-specificity analysis of 28-kda ompk from vibrio alginolyticus. | some vibrio species are universal marine pathogens and vibrio infections are often encountered due to consumption of raw or uncooked seafood. the outer membrane proteins, playing a key role in interaction between bacteria and hosts, are potential candidates for development of vaccine and markers of the genus vibrio. in this study, the ompk (outer membrane protein k) genes of vibrio alginolyticus, v. vulnificus, v. parahaemolyticus, v. fluvialis, and v. mimicu were cloned with 798 to 822 nucleoti ... | 2010 | 20546410 |
| molecular characterization and expression analysis of cathepsin l1 cysteine protease from pearl oyster pinctada fucata. | cathepsin l is one of the crucial enzyme superfamilies and involved in the immune responses. in this study, a cdna encoding cathepsin l cysteine protease was identified and characterized from pearl oyster pinctada fucata (designated as pocl1). the pocl1 cdna was 1160 bp long and consisted of a 5'-untranslated region (utr) of 15 bp, a 3'-utr of 149 bp with a polyadenylation signal (aataaa) at 11 nucleotides upstream of the poly(a) tail, and an open reading frame (orf) of 996 bp encoding a polypep ... | 2010 | 20573562 |
| membrane region m2c2 in subunit ktrb of the k+ uptake system ktrab from vibrio alginolyticus forms a flexible gate controlling k+ flux: an electron paramagnetic resonance study. | transmembrane stretch m(2c) from the bacterial k(+)-translocating protein ktrb is unusually long. in its middle part, termed m(2c2), it contains several small and polar amino acids. this region is flanked by the two alpha-helices m(2c1) and m(2c3) and may form a flexible gate at the cytoplasmic side of the membrane controlling k(+) translocation. in this study, we provide experimental evidence for this notion by using continuous wave and pulse epr measurements of single and double spin-labeled c ... | 2010 | 20573964 |
| the type iii secretion system of vibrio alginolyticus induces rapid apoptosis, cell rounding and osmotic lysis of fish cells. | vibrio alginolyticus is a gram-negative bacterium and has been recognized as an opportunistic pathogen in humans as well as marine animals. however, the virulence mechanisms for this species of vibrio have not been elucidated. this study characterized multiple mechanisms that induce cell death in fish cells upon infection with a v. alginolyticus strain, zjo. the bacterium required its type iii secretion system (t3ss) to cause rapid death of infected fish cells. dying cells exhibited some feature ... | 2010 | 20576689 |
| tumor suppressor qm-like gene from disk abalone (haliotis discus discus): molecular characterization and transcriptional analysis upon immune challenge. | we describe molecular characterization and transcriptional analysis of the gene encoding tumor suppressor qm-like protein, abqm, in the disk abalone haliotis discus discus. the full-length cdna (765-bp) of abqm was found to consist of a 654-bp orf coding for a 218 amino acid protein of a 25 kda molecular mass with a 10.2 isoelectric point. analysis of abqm sequence revealed the presence of characteristic motifs, including the ribosomal protein l10 signature, sh3-binding motif and two antibiotic ... | 2010 | 20580829 |
| deletion of valr, a homolog of vibrio harveyiś luxr generates an intermediate colony phenotype between opaque/rugose and translucent/smooth in vibrio alginolyticus. | a previous study has shown that vibrio alginolyticus zj-51 undergoes colony phase variation between opaque/rugose (op) and translucent/smooth (tr). the ai-2 quorum-sensing master regulator valr, a homolog to v. harveyi luxr, was suggested to be involved in the transition. to investigate the role of valr in the variation and in biofilm formation, an in-frame deletion of valr in both op and tr backgrounds was carried out. the mutants in both backgrounds showed an intermediate colony morphotype, wh ... | 2010 | 20582761 |
| immune response in lutjanus erythropterus induced by the major outer membrane protein (ompu) of vibrio alginolyticus. | the outer membrane proteins (omps) of the marine aquatic animal pathogen vibrio alginolyticus play an important role in the virulence of the bacterium and are potential candidates for vaccine development. in this study, the major 35.6 kda omp of v. alginolyticus was isolated by gel excision from the crude omp fraction from v. alginolyticus. the sequence of the first 27 amino acid residues from the n-terminal end of the protein is atv ykd ggt ell vgg rve frg dfi gsd, which has high homology with ... | 2010 | 20597431 |
| molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab portunus trituberculatus. | serine proteinase homologues (sphs), as one of prophenoloxiase-activating factors (ppafs), play critical roles in innate immunity of crabs. based on an est from the eyestalk full length cdna library, the complete cdna (designated as ptsph) and genomic dna of sph from the swimming crab portunus trituberculatus were cloned in this study. the estimated molecular weight of mature ptsph (354 amino acids) was 38.7 kda and its isoelectric point was 5.08. multiple sequence alignment revealed that ptsph ... | 2010 | 20633654 |
| screening and selection of stress resistant lactobacillus spp. isolated from the marine oyster (crassostrea gigas). | we attempted to isolate lactobacillus spp. from the marine oyster (crassostrea gigas) and select stress resistant strains for development of a future marine aquaculture feed adjuvant. a total of 83 lactobacilli strains were isolated from oyster. they were all gram-positive, rod-shaped and catalase-negative. by performing a stress resistance assay, we selected eighteen isolates. based on 16s rrna gene sequencing, lactobacillus paracasei was the most prevalent species among the selected isolates. ... | 2010 | 20633689 |
| evaluation of immune response and resistance to diseases in tiger shrimp, penaeus monodon fed with biofilm of vibrio alginolyticus. | immune response in juvenile tiger shrimp, penaeus monodon fed with biofilm (bf) and free cells (fc) of vibrio alginolyticus was studied by evaluating the hemocyte count, phenoloxidase activity and antibacterial activity. the above immune responses were higher in bf fed shrimp than that in fc fed or control shrimp. among the different doses of bf of v. alginolyticus tested, 10(9) cfu g(-1) shrimp day(-1) for two weeks could evoke higher immune response. bf fed shrimp were more resistant to inject ... | 2010 | 20638481 |
| identification of vaccine candidates from differentially expressed outer membrane proteins of vibrio alginolyticus in response to nacl and iron limitation. | vibrio alginolyticus is the etiological agent that causes great losses in aquacultures and clinical emanating cases in humans. identification of highly efficient vaccine candidates to control v. alginolyticus infection has been highly concerned since vaccines offer a powerful approach to provide efficient protection from bacterial infections. in the present study, we firstly investigated the altered outer membrane proteins (om proteins) of v. alginolyticus in response to nacl concentrations and ... | 2010 | 20659563 |
| expression, purification, and characterization of thermolabile hemolysin (tlh) from vibrio alginolyticus. | hemolysin is a putative pathogenicity factor in many bacterial pathogens. in this study, a dna fragment containing the open reading frame (1254 bp) of the thermolabile hemolysin gene (tlh) from vibrio alginolyticus v05 was amplified and cloned into the expression plasmid pet-24d(+). the deduced amino acid sequence of the thermolabile hemolysin (tlh) shared 94 and 83% identity with the lecithin-dependent hemolysin (ldh)/tlh of v. parahaemolyticus and v. harveyi thermolabile hemolysin (vhh), respe ... | 2010 | 20662368 |
| comparative genomic analyses identify the vibrio harveyi genome sequenced strains baa-1116 and hy01 as vibrio campbellii. | three notable members of the harveyi clade, vibrio harveyi, vibrio alginolyticus and vibrio parahaemolyticus, are best known as marine pathogens of commercial and medical import. in spite of this fact, the discrimination of harveyi clade members remains difficult due to genetic and phenotypic similarities, and this has led to misidentifications and inaccurate estimations of a species' involvement in certain environments. to begin to understand the underlying genetics that complicate species leve ... | 2010 | 20686623 |
| brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna from disk abalone haliotis discus discus. | a brichos domain-containing leukocyte cell-derived chemotaxin 1-like cdna was cloned from the disk abalone (haliotis discus discus) and designated as ablect-1. a full-length (705 bp) of ablect-1 cdna was composed of a 576 bp open reading frame that translates into a putative peptide of 192 amino acids. deduced amino acid sequence of ablect-1 had 15.5- and 27.8% identity and similarity to human lect-1, respectively. quantitative real-time pcr analysis results showed that the mrna of ablect-1 was ... | 2010 | 20688173 |
| gain of function mutations in membrane region m2c2 of ktrb open a gate controlling k+ transport by the ktrab system from vibrio alginolyticus. | ktrb, the k(+)-translocating subunit of the na(+)-dependent bacterial k(+) uptake system ktrab, consists of four m(1)pm(2) domains, in which m(1) and m(2) are transmembrane helices and p indicates a p-loop that folds back from the external medium into the cell membrane. the transmembrane stretch m(2c) is, with its 40 residues, unusually long. it consists of three parts, the hydrophobic helices m(2c1) and m(2c3), which are connected by a nonhelical m(2c2) region, containing conserved glycine, ala ... | 2010 | 20097755 |