Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| regulatory mutations in the klebsiella aerogenes structural gene for glutamine synthetase. | glutamine synthetase could be repressed several hundredfold rather than 6- to 10-fold as previously reported. ammonia was not the primary repression signal for glutamine synthetase. repression appeared to be mediated by a high level of glutamine and probably by a high ratio of glutamine to alpha-ketoglutarate. mutations in glna (the structural gene for glutamine synthetase) were seen to fall into three phenotypic groups: glutamine auxotrophs that produced no detectable glna product; glutamine au ... | 1977 | 21157 |
| autogenous regulation of the synthesis of glutamine synthetase in klebsiella aerogenes. | we isolated an f' episome of escherichia coli carrying the glna+ gene from k. aerogenes and an f' episome of e. coli carrying the glna4 allele from k. aerogenes responsible for the constitutive synthesis of glutamine synthetase. complementation tests with these episomes showed that the glna4 mutation (leading to the constitutive synthesis of active glutamine synthetase) was in the gene identified by mutations glna20, glna51, and glna5 as the structural gene for glutamine synthetase. by using the ... | 1977 | 21158 |
| biochemical parameters of glutamine synthetase from klebsiella aerogenes. | the glutamine synthetase (gs) from klebsiella aerogenes is similar to that from escherichia coli in several respects: (i) it is repressed by high levels of ammonia in the growth medium; (ii) its biosynthetic activity is greatly reduced by adenylylation; and (iii) adenylylation lowers the ph optimum and alters the response of the enzymes to various inhibitors in the gamma-glutamyl transferase (gammagt) assay. there are, however, several important differences: (i) the isoactivity point for the ade ... | 1977 | 14104 |
| regulation of glna messinger ribonucleic acid synthesis in klebsiella aerogenes. | we examined wild-type and mutant strains of klebsiella aerogenes for the relative amounts of ribonucleic acid (rna) hybridizing specifically to deoxyribonucleic acid from a transducing phage carrying glnak, the structural gene for glutamine synthetase. our data showed a positive correlation between the intracellular level of glutamine synthetase and the level of glna messenger rna; we were unable to detect glna messinger rna in strains devoid of glutamine synthetase protein. therefore, it is pos ... | 1977 | 14114 |
| glutamine synthetase of klebsiella aerogenes: genetic and physiological properties of mutants in the adenylylation system. | mutations resulting in defects in the adenylylation system of glutamine synthetase (gs) affect the expression of glna, the structural gene for gs. mutants with lesions in glnb are glutamine auxotrophs and contain repressed levels of highly adenylylated gs. glutamine-independent revertants of the glnb3 mutant have acquired an additional mutation at the glne site. the glne54 mutant is incapable of adenylylating gs and produces high levels of enzyme, even when ammonia is present in the growth mediu ... | 1977 | 14117 |
| evidence for regulation of aerobacter aerogenes ph 6 acetolactate-forming enzyme by acetate ion. | 1977 | 14639 | |
| lack of a regulatory function for glutamine synthetase protein in the synthesis of glutamate dehydrogenase and nitrite reductase in escherichia coli k12. | synthesis of glutamine synthetase (gs) in anaerobic batch cultures of escherichia coli was repressed when excess nh4+ was available, but derepressed during growth with a poor nitrogen source. in wild-type bacteria there was only a weak inverse correlation between the activities of gs and glutamate dehydrogenase (gdh) during growth in various media. no positive correlations were found between the activities of gs and nitrite reductase, or between gs and cytochrome c552: both of these proteins wer ... | 1977 | 16079 |
| regulation of enzyme synthesis by the glutamine synthetase of salmonella typhimurium: a factor in addition to glutamine synthetase is required for activation of enzyme formation. | in klebsiella aerogenes but not in salmonella typhimurium glutamine synthetase can function during nitrogen-limited growth to increase the rate of synthesis of histidase from the hut genes of s. typhimurium 15-59 (huts. 15-59). formation of proline oxidase is also not increased in nitrogen-limited cultures of s. typhimurium. however, in hybrid strains of escherichia coli or k. aerogenes, the glutamine synthetase of s. typhimurium activates synthesis of histidase from the huts. 15-59 genes. appar ... | 1977 | 16868 |
| gentamicin-resistant klebsiella aerogenes in a urological ward. | a gentamicin-resistant strain of klebsiella aerogenes was isolated from the urine of 17 patients out of 237 admitted to a male urological ward between jan. 21 and may 9, 1977. the factors most frequently associated with k. aerogenes in the urine were catheterisation and antibiotic therapy. often the epidemic strain (type k16) was found not only in the patients' faeces but also on more remote skin sites such as hands, knees, groins, and the umbilicus. resistance to gentamicin and many other antib ... | 1977 | 70654 |
| immunological study of the regulation of cellular arylsulfatase synthesis in klebsiella aerogenes. | regulation of cellular arylsulfatase synthesis in klebsiella aerogenes was analyzed by immunological techniques. antibody directed against the purified arylsulfatase from k. aerogenes w70 was obtained from rabbits and characterized by immunoelectrophoresis, double-diffusion, quantitative precipitation, and enzyme neutralization tests. arylsulfatase was located in the periplasmic space when the wild-type strain was cultured with methionine or with inorganic sulfate plus tyramine, but not with ino ... | 1977 | 72063 |
| purification and initial rate kinetics of acyl-phosphate-hexose phosphotransferase from aerobacter aerogenes. | the enzyme acyl-phosphate-hexose phosphotransferase from aerobacter aerogenes was purified to electrophoretic homogeneity. the molecular weight of the enzyme as determined on sephadex gels is 150 000. the enzyme possesses potent phosphotransferase and phosphohydrolase activities. initial rate kinetics were used to investigate the mechanism of acyl-phosphate-hexose phosphotransferase. these studies, which involved a number of different phosphoryl donors and substrate analogues, suggest that the k ... | 1977 | 196625 |
| on the structure of the prosthetic group of citrate (pro-3s)-lyase. | the prosthetic group of citrate (pro-3s)-lyase from klebsiella aerogenes as well as streptococcus diacetilactis was obtained eigher by beta elimination or pronase digestion of the enzyme and purified by deae-cellulose chromatography. the compound was shown to contain 3 mol of po4, 2 mol of ribose, and 1 mol of sulfhydryl/mol of adenine. 5'-amp and dephospho-coa are components of the prosthetic group. the evidence obtained so far support our proposed structure of 3' (or 2') leads to 1''-(5''-phos ... | 1977 | 197081 |
| intracellular distribution of enzymes of phospholipid metabolism in several gram-negative bacteria. | cell-free extracts of salmonella typhimurium, serratia marcescens, enterobacter aerogenes, and micrococcus cerificans contained the following enzymatic activities related to phospholipid metabolism: cytidine 5'-diphospho-1,2-diacyl-sn-glycerol (cdp-diglyceride):l-serine o-phosphatidyltransferase (phosphatidylserine synthase), phosphatidylserine decarboxylase, cdp-diglyceride:sn-glycero-3-phosphate phosphatidyltransferase (phosphatidylglycerophosphate synthase), phosphatidylglycerophosphate phosp ... | 1977 | 199573 |
| preferential uptake of thymidine by thymineless enterobacteria: its significance in dna labelling. | minimum satisfactory concentrations of thymine and thymidine were determined for the growth of a high thymine-requirng (thy) mutant to escherichia coli strain j5-3. cultures were then grown in the presence of these concentrations of non-radioactive ('cold') pyrimidine together with 5 microci/ml [methyl-3h)thymine, or [methyl-3h)thymidine (specific activities 5 ci/m mole), and the uptake of radioactivity into ice cold trichloroacetic acid insoluble material determined. by far the most efficient l ... | 1977 | 357930 |
| purification and specificity of bacteriolytic enzyme i from hartmannella glebae. | a soil amoeba (hartmannella glebae), when grown in conjunction with enterobacter aerogenes and alcaligenes faecalis, produced two enzymes. enzyme i was purified by gel filtration on sephadex g-100 and chromatography on diethylaminoethyl-cellulose. it is a basic protein. the analysis of the enzymic digest of the cell walls of micrococcus lysodeikticus after reduction and acid hydrolysis showed that the enzyme cleaved the glycosidic bond between acetylmuramic acid and acetylglucosamine of the pept ... | 1977 | 402108 |
| immunochemical comparison of phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase among the enterobacteriaceae. | the bifunctional enzyme of the tryptophan operon, phosphoribosylanthranilate isomerase-indoleglycerol phosphate synthetase (prai-ingps;ec 4.1.1.48), was characterized by an immunochemical study of six representative members of the enterobacteriaceae: escherichia coli, salmonella typhimurium, enterobacter aerogenes, serratia marcescens, erwinia carotovora, and proteus vulgaris. prai-ingps was purified from e. coli, and antisera were prepared in rabbits. these antisera were utilized in quantitativ ... | 1977 | 403178 |
| immunochemical evidence for the difference between coenzyme-b12-dependent diol dehydratase and glycerol dehydratase. | klebsiella pneumoniae atcc 25955 (formerly named aerobacter aerogenes pzh 572, warsaw), which is known to produce coenzyme-b12-dependent glycerol dehydratase when grown anaerobically in a glycerol medium, formed coenzyme-b12-dependent diol dehydratase in a 1,2-propanediol-containing medium. both the diol dehydratase and the glycerol dehydratase produced by the organism catalyzed the conversion of glycerol, 1,2-propanediol and 1,2-ethanediol to the corresponding aldehydes and underwent concomitan ... | 1977 | 407082 |
| immunization with r mutants of salmonella minnesota. ii. comparison of the protective effect of immunization with lipid a and the re mutant. | the protective effect of active immunization with salmonella minnesota re bacilli or lipid a was assessed in the granulocytopenic rabbit model. animals were immunized with re bacilli, lipid a, or pseudomonas aeruginosa as a nonspecific immunogen. after colonization with one of three enterobacterial strains (two escherichia coli, one enterobacter aerogenes), the immunized rabbits as well as controls given saline injections were made leukopenic with nitrogen mustard and monitored for fever, bacter ... | 1977 | 407161 |
| noxythiolln-resistant organisms in a district general hospital. | twelve strains of pseudomonas aeruginosa, three strains of klebsiella aerogenes, and two strains of escherichia coli were found to be resistant to noxythiolin. some of the pseudomonads were isolated from patients in the same ward, not all of whom were on noxythiolin treatment. the strains from these patients were indistinguishable from each other on phage typing, which suggested cross-contamination. no gram-positive organism was found to be resistant to noxythiolin. noxythiolin should not be use ... | 1977 | 412549 |
| respiratory infection in an intensive care unit. | a postmortem bacteriological study of black children in a respiratory intensive care unit showed that pseudomonas aeruginosa was the most common opportunistic pathogen and that it usually complicated a viral infection. in a parallel study of non-debilitated patients in general hospital wards klebsiella aerogenes and escherichia coli were the most frequently isolated organisms. counter-immuno-electrophoresis was used for the identification of pseudomonas-precipitating antibody in serum and trache ... | 1977 | 416503 |
| fatty acid compositions of lipid fractions from vegetative cells and mature sorocarps of the cellular slime mold dictyostelium discoideum. | a wild-type strain of dictyostelium discoideum was grown upon aerobacter aerogenes. fatty acid compositions of lipid fractions and of total lipids obtained from vegetative amoebae and mature sorocarps were compared. fatty acids isolated from vegetative cells were found to include large quantities of 17- and 19-carbon cyclopropane fatty acids while straight-chain, saturated fatty acids represented only 10% (w/w) of total fatty acids. these cyclopropane fatty acids appear to be derived from ingest ... | 1977 | 559228 |
| phenotypic modifications in amino acid profiles of cell residues of candida utilis and enterobacter aerogenes. | glucose-limited chemostat cultures of candida utilis were cultivated at various ph levels (3.0-7.5), temperatures (15-37.5 degrees c), dilution rates (0.006-0.42 hr-1), and with one of two nitrogen sources (nh+4 or no-3). enterobacter aerogenes was also cultivated in the chemostat under nitrogen and phosphorus limitations. the amino acid profile of total cell protein is expressed as the content of each amino acid relative to the sum of all amino acids recovered ater acid hydrolysis. cell residue ... | 1977 | 560224 |
| laboratory studies on mecillinam: activity alone and combined with cephradine. | mecillinam (mc) was found to be inhibitory at low concentrations to a wide range of gram-negative bacteria; the sensitivity of such strains could be predicted by using a 10 microgram disk. resistance to mc did not appear to be associated with beta-lactamase-mediated destruction of the antibiotic. ampicillin-resistant strains tended to be less sensitive to mc. mc and cephradine acted synergistically against proteus mirabilis, pr. vulgaris, prov. stuartii and serratia marcescens, and the combinati ... | 1977 | 587708 |
| [9-(adenylyl)alkylcobalamins as inhibitors of adenosylcobalamin-dependent glycerol dehydratase from aerobacter aerogenes]. | the behavior of two coenzyme analogs, [(5-aden-9-yl)methoxyethyl] cob (iii) alamin and [(5-aden-9-yl)pentyl] cob (iii) alamin modified at the nucleoside ligand sugar moiety was studied in the system of adenosyl-cobalamin-dependent glycerol dehydratase from aerobacter aerogenes. it was shown that neither of the analogs possesses coenzyme properties and that both are strong competitive inhibitors for adenosylcobalamin (adocbl). the affinity of the two analogs for the apoenzyme is higher than that ... | 1977 | 588639 |
| microbial conversion of dl-2-amino-delta2-thiazoline-4-carboxylic acid to l-cysteine and l-cystine: screening of microorganisms and identification of products. | microorganisms able to form l-cysteine from dl-2-amino-delta2-thiazoline-4-carboxylic acid (dl-atc), a chemical intermediate in the synthesis of dl-cysteine, were isolated from soil samples and classified as pseudomonas sp., pseudomonas cohaerens, p. desmolytica, and p. ovalis. thirteen l-cysteine-producing bacteria were also found in among 463 stock cultures representing 37 genera. these were achromobacter delmarvae. alcaligenes denitrificans, bacillus brevis, brevibacterium flavum, enterobacte ... | 1977 | 596877 |
| specific gonadotropin binding to pseudomonas maltophilia. | binding of 125i-labeled human chorionic gonadotropin to pseudomonas maltophilia is dependent on time, temperature, and ph and the binding to this procaryotic species is hormone-specific and saturable. the equilibrium dissociation constant is 2.3 x 10(-9) m. there are no cooperative interactions between binding sites (hill coefficient, 1.05). the number of sites is estimaated as 240 fmol/100 mug of protein. nacl and kcl, at concentrations from 1 to 10 mm, have no effect on binding. divalent catio ... | 1977 | 265583 |
| assymetry of the myo-inositol transport system in klebsiella aerogenes. energy is necessary to create the assymetry of the transport system. | 1. in klebsiella aerogenes the influx of myo-inositol proceeds during the steady state at a rate equal to that of efflux. 2. the kinetic parameters of influx during the steady state are similar to those observed at the initial time of uptake. 3. efflux and influx processes share the same transport system. 4. the ability of other cyclitols to chase the accumulated scyllo-inositol is dependent on their affinity for the transport system. 5. a counter transport can be observed in preloaded cells onl ... | 1977 | 318995 |
| myo-inositol transport in klebsiella aerogenes. scyllo-inositol, a non-metabolizable substrate for the study of the myo-inositol transport system. | 1. scyllo-inositol is transported against a concentration gradient by klebsiella aerogenes, but is not metabolized. 2. the apparent kt of scyllo-inositol is 0.05 x 10(-3) m while that of myo-inositol is 0.23 x 10(-3) m. the v values are respectively 32 and 12 nmol min(-1) mg cells(-1) (dry weight). 3. both cyclitols bind to the same carrier, since typical competitive inhibition kinetics are observed. 4. neither phosphoenolpyruvate phosphotransferase nor a periplasmic binding protein seems to be ... | 1977 | 319004 |
| excystment of axenically prepared cysts of hartmanella culbertsoni. | axenically prepared cysts of hartmannella culbertsoni readily excysted in the presence of heat stable factors prepared from escherichia coli, klebsiella aerogenes, staphylococcus aureus, sarcina lutea, bacillus subtilis, bacillus megaterium and several fungi. peptone, proteose peptone, tryptone or amino acids also promoted excystment. crowding of the cysts and dilution of bacterial extracts adversely affected the excystment. continual presence of the factors in the medium was essential for excys ... | 1977 | 319194 |
| taxonomy of the genus serratia. | one hundred and fifty-six strains of serratia and related bacteria including representatives of enterobacter liquefaciens, enterobacter cloacae, enterobacter aerogenes, erwinia carotovora, erwinia chrysanthemi, erwinia herbicola and erwinia nimipressuralis were studied using 223 morphological, physiological, biochemical and carbon source utilization tests. the results were subjected to computer analysis. at the 80% similarity level all strains, except two, grouped into eight phenons representing ... | 1977 | 319202 |
| coliforms from hides and meat. | coliform tests were performed on 85 hide and 75 meat samples. imvic reactions were determined on isolates from positive confirmed and fecal tests, and strains other than escherichia coli were identified. strains typed as aerobacter aerogenes types i and ii were identified as enterobacter cloacae (51.4%), klebsiella pneumoniae (21.5%), enterobacter aerogenes (15%), and enterobacter liquefaciens, serratia, and unidentified coliforms (12.1%). k. pneumoniae appeared to be responsible for less than 1 ... | 1977 | 319754 |
| studies on the mechanism of the adenosylcobalamin-dependent diol dehydrase reaction by the use of analogs of the coenzyme. | a series of 16 analogs of 5'-deoxy-5'-adenosylcobalamin (adenosylcobalamin) were examined for their effects on the diol dehydrase system of klebsiella pneumoniae (aerobacter aerogenes). four analogs, ara-adenosyl-, aristeromycyl-, 3-isoadenosyl-, and nebularylcobalamin, were able to function as coenzymes in the diol dehydrase reaction, coenzyme activity decreasing in that order. like the native holoenzyme, complexes of the enzyme with these four analogs show a cob(ii)alamin-like absorption peak ... | 1977 | 320203 |
| novel r-plasmid-mediated beta-lactamase from klebsiella aerogenes. | a novel beta-lactamase with a broad spectrum of activity against penicillins and cephalosporins has been detected in a strain of klebsiella aerogenes. its synthesis is mediated by an r-plasmid of molecular weight 64 x 10(6). | 1977 | 329756 |
| isolation of poly-alpha-l-guluronate lyase from klebsiella aerogenes. | the bacterium klebsiella aerogenes type 25 produces an extracellular alginolyase which has been partly purified. the enzyme is specific for the alpha-l-guluronosyl linkages in whole alginate and fractions therefrom. the end products of its action on polyguluronic acid blocks are mainly the unsaturated di- and tri-saccharides, with a smaller proportion of the homologous tetrasaccharide. some general properties of the enzyme are reported. | 1977 | 332364 |
| experience with amikacin and colistin in an outbreak of infection by resistant klebsiella aerogenes. | 1977 | 332682 | |
| explusion of salibi-clamp from the internal carotied artery in the neck by infection. | a left infraclinoid carotid aneurysm (3 x 2-5 x 2 cm) in a 60-year old woman presented with total ophthalmoplegia and trigeminal symptoms. a staged internal carotied occlusion in the neck was performed using a salibi-clamp. a chronic infectious fistula developed (klebsiella aerogenes). when superficial debridement failed, operative removal of the salibi-clamp was planned seven months after its insertion. the clamp was found subcutaneously without any relation to the internal carotid artery and w ... | 1977 | 333303 |
| affinity chromatography od klebsiella arylsulfatase on tyrosyl-hexamethylenediamine-beta-1,3-glucan and immunoadsorbent. | a simple and convenient method for preparation of a highly purified arylsulfatase (ec 3.1.6.1) from klebsiella aerogenes has been developed. specificity of purification was achieved by using affinity chromatography on a tyrosyl-hexamethylenediamino-beta-1,3-glucan or on a solid phase immunoadsorbent. by using affinity chromatography a homogeneous enzyme was obtained with high yield. it is also proposed that the beads of curdlan type polysaccharide consisting of beta-1,3-glucan can be used as a g ... | 1977 | 334267 |
| bacteriology of abscesses of the central nervous system: a multicentre prospective study. | pus from 46 patients with abscesses of the central nervous system (cns) was examined for bacteria; bacteria were found in all patients. streptococci were isolated from 36 patients and most isolates were streptococcus milleri, lancefield group f, ottens and winkler type o iii. staphylococci were isolated from nine patients, organisms of the bacteroides group from 11, proteus spp from seven, klebsiella aerogenes from one, and haemophilus aphrophilus from one. pure cultures predominated over mixed ... | 1977 | 336141 |
| a comparison of alternate metabolic strategies for the utilization of d-arabinose. | mutants of klebsiella aerogenes w70 that metabolize the uncommon pentose d-arabinose were isolated. these mutants were found to be either constitutive or indicible by d-arabinose for the synthesis of enzymes in the l-fucose pathway. such mutants could then utilize l-fucose isomerase to convert the structurally similar d-arabinose molecule to d-ribulose. d-ribulose is an intermediate and the inducer of an existing ribitol pathway and could thus be metabolized. in those d-arabinose-positive mutant ... | 1977 | 338926 |
| hexokinase and glucose-phosphoenolpyruvate phosphotransferase synthesis in klebsiella aerogenes strains growing in continuous culture. | considerable differences in steady-state hexokinase specific activity were found in 16 n.c.i.b. strains of klebsiella aerogenes grown in identical conditions in glucose-limited chemostats. strains of n.c.i.b. 8258 had no detectable activity, but its glucose-phosphoenolpyruvate phosphotransferase specific activity and that of the other strains were closely similar, and it is concluded that this phosphotransferase activity regulates the overall utilization of glucose, in which hexokinase plays no ... | 1977 | 339911 |
| pathophysiologic responses to klebsiella aerogenes bacteria: 24-hour observations. | 1977 | 364736 | |
| tyramine oxidase and regulation of arylsulfatase synthesis in klebsiella aerogenes. | the participation of tyramine oxidase in the regulation of arylsulfatase synthesis in klebsiella aerogenes was studied. arylsulfatase was synthesized when this organism was grown with methionine or taurine as the sulfur source (nonrepressing conditions) and was repressed by inorganic sulfate or cysteine; this repression was relieved by tyramine and related compounds (derepressing conditions). under nonrepressing conditions, arylsulfatase synthesis was not regulated by tyramine oxidase synthesis. ... | 1977 | 830648 |
| intracellular crystalline deposits by bacteria grown in urine from a stone former. | several species of bacteria were found to form an intracellular crystalline material when grown in urine obtained from a subject with a history of infrequent renal calculi formation. the following species: proteus mirabilis, proteus rettgeri, providencia stuartii, enterobacter aerogenes, enterobacter cloacae, escherichia coli, and candida albicans formed crystals of hydroxyapatite. klebsiella pneumoniae, pseudomonas aeruginosa, and proteus vulgaris produced crystals of calcite -ii. several of th ... | 1977 | 844994 |
| genetic control of arylsulfatase synthesis in klebsiella aerogenes. | it was shown that at least four genes are specifically responsible for arylsulfatase synthesis in klebsiella aerogenes. mutations at chromosome site atsa result in enzymatically inactive arylsulfatase. mutants showing constitutive synthesis of arylsulfatase (atsr) were isolated by using inorganic sulfate or cysteine as the sulfur source. another mutation in which repression of arylsulfatase by inorganic sulfate or cysteine could not be relieved by tyramine was determined by genetic analysis to b ... | 1977 | 853036 |
| growth of klebsiella aerogenes on xylitol: implications for bacterial enzyme evolution. | when klebsiella aerogenes was grown in continuous culture with xylitol. an unnatural pentitol, as the growth limiting substrate, the structural gene which codes for ribitol dehydrogenase, an enzyme which gratuitously catalyzes the oxidation of xylitol to d-xylulose, was duplicated. it appears that the duplication mechansim only duplicates the gene which is subjected to selective pressure and not any of the other closely linked genes. the degree to which the ribitol dehydrogenase gene is duplicat ... | 1977 | 864722 |
| [catechol biosynthesis by klebsiella aerogenes bacteria]. | 1977 | 865322 | |
| clavulanic acid: a beta-lactamase-inhiting beta-lactam from streptomyces clavuligerus. | a novel beta-lactamase inhibitor has been isolated from streptomyces clavuligerus atcc 27064 and given the name clavulanic acid. conditions for the cultivation of the organism and detection and isolation of clavulanic acid are described. this compound resembles the nucleus of a penicillin but differs in having no acylamino side chain, having oxygen instead of sulfur, and containing a beta-hydroxyethylidine substituent in the oxazolidine ring. clavulanic acid is a potent inhibitor of many beta-la ... | 1977 | 879738 |
| the utilization of the plasticizer dimethyl phthalate by an isolated strain of enterobacter aerogenes. | 1977 | 884330 | |
| numerical taxonomy and ecology of petroleum-degrading bacteria. | a total of 99 strains of petroleum-degrading bacteria isolated from chesapeake bay water and sediment were identified by using numerical taxonomy procedures. the isolates, together with 33 reference cultures, were examined for 48 biochemical, cultural, morphological, and physiological characters. the data were analyzed by computer, using both the simple matching and the jaccard coefficients. clustering was achieved by the unweighted average linkage method. from the sorted similarity matrix and d ... | 1977 | 889329 |
| study on the mechanism of action of adenosylcobalamin-dependent glycerol dehydratase from aerobacter aerogenes. i. role of structural components of adenosylcobalamin the formation of the active site of glycerol dehydratase. | a new method of partial chemical synthesis of adenosylcobalamin (co alpha-[alpha-5,6-diemethylbenzimidazolyl)]-co beta-adenosylcobamide, adocbl) analogs has been developed. a series of derivatives of adocbl modified in the nucleoside and nucleotide ligands and corrin macrocycle have been obtained. the interaction of adocl analogs with glycerol dehydratase (ec 4.2.1.30) from aerobacter aerogenes has been investigated. it has been shown that the nucleoside ligand of adocbl provides no essential co ... | 1977 | 889845 |
| study of the mechanism of action of adenosylcobalamindependent glycerol dehydratase from aerobacter aerogenes. ii. the inactivation kinetics of glycerol dehydratase complexes with adenosylobalamin and its analogs. | the inactivation kinetics of vacterial glycerol dehydratase (ec 4.2.1.30) in the course of its reaction with adenosylcobalamin (adocbl) and its analogs were investigated. it was shown that the inactivation rate of apoenzyme complexes with adocbl analogs is determined by the nature of the analogs employed and probably by the rate of their conversion into hydroxycobalamins. a possible inactivation mechanism of glycerol dehydratase is discussed. | 1977 | 889846 |
| enzymatic and immunological characterization of a new cephalosporinase from enterobacter aerogenes. | a hospital strain of enterobacter aerogenes (mulb 250) isolated from a urinary tract infection was found to be cephalosporin and ampicillin resistant and carbenicillin susceptible. the beta-lactamase produced by this strain was extracted and purified by means of affinity chromatography, using a cephalosporin c-bound sepharose 4b column. the purified enzyme was tested for hydrolysis of penicillin and various cephalosporins. the k(m) value is 11.8 mum for benzyl penicillin and 130 mum for cephalos ... | 1977 | 900918 |
| in vitro activities of five oral cephalosporins against aerobic pathogenic bacteria. | cefaclor (lilly 99638) and cefatrizine (bl-s640, sk&f 70771) are orally absorbed, broad-spectrum semisynthetic cephalosporins. they were compared in vitro with cephalexin, cephaloglycin, and cepharadine against a variety of aerobic pathogenic bacteria by an agar dilution procedure. cefaclor and cefatrizine were found to be similar or superior to cephalexin, cephaloglycin, and cephradine in terms of activity against gram-positive cocci other than enterococci. only cefatrizine demonstrated any pot ... | 1977 | 921258 |
| purification and properties of citrate lyase ligase from streptococcus diacetilactis. | citrate lyase ligase (acetate: sh--[acyl-carrier protein] enzyme ligase (amp) from streptococcus diacetilactis was purified 920-fold with a yield of 6.3%. the molecular weight of the enzyme was estimated to be 41000; the ligase consisted of one polypeptide chain. the acetylation of 1 mol of deacetyl-citrate lyase to enzymatically active citrate lyase required 6 mol atp. the formation of amp and pyrophosphate in the acetylation reaction was demonstrated. citrate lyase ligase was specific for the ... | 1977 | 923578 |
| energy-dependent inactivation of citrate lyase in enterobacter aerogenes. | enterobacter aerogenes was grown in continous culture with ammonia as the growth-limiting substrate, and changes in citrate lyase and citrate synthase activities were monitored after growth shifts from anaerobic growth on citrate to aerobic growth on citrate, aerobic growth on glucose, anaerobic growth on glucose, and anaerobic growth on glucose plus nitrate. citrate lyase was inactivated during aerobic growth on glucose and during anaerobic growth with glucose plus nitrate. inactivation did not ... | 1977 | 924971 |
| transport equations for a microbial predator-prey community. | a transport equation is used which describes the temporal behavior of interacting populations in changing environments. the formulation takes into account the internal state variables of the individuals. the general theory is applied to the transient analysis of a microbial predator-prey system using an approximate model for the specific cell growth rate and multigroup formulism to approximate the mass distribution within the population. experimental results in atetrahymena pyriformis- aerobacte ... | 1977 | 24233466 |
| a hospital epidemic caused by gentamicin-resistant klebsiella aerogenes. | in the 15 months, february 1976 to april 1977, more than 241 patients became colonized with a strain of klebsiella aerogenes, capsular serotype k2, resistant to most antibiotics. urinary tract infection was the most common clinical manifestation but bacteraemia and, occasionally, infections of other sites were encountered. the main reservoir of the epidemic klebsiella was the gut, urine and skin of colonized patients. gut carriage among staff was very uncommon. the most susceptible patients were ... | 1978 | 340580 |
| utilization of arginine by klebsiella aerogenes. | klebsiella aerogenes utilized arginine as the sole source of carbon or nitrogen for growth. arginine was degraded to 2-ketoglutarate and not to succinate, since a citrate synthaseless mutant grows on arginine as the only nitrogen source. when glucose was the energy source, all four nitrogen atoms of arginine were utilized. three of them apparently did not pass through ammonia but were transferred by transamination, since a mutant unable to produce glutamate by glutamate synthase or glutamate deh ... | 1978 | 342501 |
| evaluation and application of an improved bacteriocin typing method for klebsiella aerogenes. | a bacteriocin typing method was evaluated using 200 strains of klebsiella aerogenes, 93% of which fell into 11 distinct types. the typing technique was successfully applied to the monitoring and control of hospital cross-infection. | 1978 | 342545 |
| action of pseudomonas isoamylase on various branched oligo and poly-saccharides. | pseudomonas isoamylase (ec 3.2.1.68) hydrolyzes (1 linked to 6)-alpha-d-glucosidic linkages of amylopectin, glycogen, and various branched dextrins and oligosaccharides. the detailed structural requirements for the substrate are examined qualitatively and quantitatively in this paper, in comparison with the pullulanase of klebsiella aerogenes. as with pullulanase, ps. isoamylase is unable to cleave d-glucosyl stubs from branched saccharides. ps. isoamylase differs from pullulanase in the followi ... | 1978 | 348322 |
| effect of oxygen supply on growth of klebsiella aerogenes in a multistage tower fermentor. | the effect of the rate of oxygen supply on biomass growth, consumption of carbon source formation of metabolic by-products, biomass yeilds referred to c-source and oxygen, respiration rate and the respiratory quotient was studied in a multistage tower fermentor with an interstage backflow, i.e. with a continuous reinoculation of the preceding stages. experiments were done with klebsiella aerogenes ccm 2318 in a synthetic glucose medium with constant glucose concentration in the feed, at ph 7.0. ... | 1978 | 348585 |
| bowls and bacteria. | an episode of nosocomial infection with klebsiella aerogenes in a surgical ward, in which six patients were infected, is described. the cause of the outbreak was identified as being contaminated washing-bowls. it is recommended that each patient should have his own bowl, which should be disinfected after use, dried and stored upside-down. | 1978 | 349076 |
| on the significance of the prosthetic group composition of citrate lyase. | 1. klebsiella aerogenes contains two different acyl carrier proteins, one specific for citrate lyase, the other for fatty acid synthetase. 2. the acyl carrier protein of fatty acid synthetase from k. aerogenes was isolated and compared with the corresponding protein from escherichia coli and with the acyl carrier protein of citrate lyase from k. aerogenes. 3. as judged from prosthetic group compositions as well as amino acid and fingerprint analyses, the acyl carrier proteins of the two fatty ac ... | 1978 | 350581 |
| selective inhibition of klebsiella aerogenes growth on pentoses by pentitols. | selective inhibition of growth by pentitols was observed when klebsiella aerogenes m-7 which could not utilize pentitols was grown on pentoses. d-arabitol inhibited the growth on d-arabinose as a sole carbon source, but had no effect on the growth on l-arabinose, d-xylose, and d-ribose. similarly, l-arabitol inhibited the growth on d-arabinose and l-arabinose, ribitol inhibited the growth on d-arabinose and l-arabinose, and xylitol inhibited the growth on d-xylose. from the following reasons, we ... | 1978 | 350845 |
| [severe klebsiella serogenes infections]. | it is reported on 2 cases of klebsiella-septikaemia with difficult course. germs of the group klebsiella aerogenes could be proved microbiologically. on the basis of the peculiarities of the clinical course the author adopts a definite attitude to the development of the infection, the pathogenesis, to the microbiological proof and to the aimed antimicrobial chemotherapy. the antibiotic treatment was successful in the first case with karbenicillin and gentamycin and in the second case with cephal ... | 1978 | 351992 |
| amino-acid sequence of citrate-lyase acyl-carrier protein from klebsiella aerogenes. | 1978 | 352686 | |
| differentiation of serratia marcescens and serratia liquefaciens by tests for lipase and phospholipase production. | the production of lipase and phospolipase by certain members of the enterobacteriaceae was examined by thin-layer chromatography of resting-cell suspensions incubated with triolein or lecithin. most strains of serratia marcescens produced both enzymes while most strains of serratia liquefaciens exhibited strong lipase but only a minor phospholipase activity. enterobacter spp. (25 strains), klebsiella pneumoniae (20 strains), escherichia coli (15 strains), citrobacter freundii (7 strains) and pro ... | 1978 | 355631 |
| the effects of temperature and growth rate on the proportion of unsaturated fatty acids in bacterial lipids. | the effects of temperature and growth rate on the fatty acid composition of the extractable lipids of four mesophilic and three psychotrophic bacteria were examined. two of the mesophiles (escherichia coli and pseudomonas aeruginosa) increased the proportion of unsaturated fatty acids in their lipids with decreasing temperature over their whole growth temperature range. the other mesophiles (enterobacter aerogenes and lactobacillus casei) increased the proportion of unsaturated fatty acids with ... | 1978 | 624935 |
| comparative in vitro activity of five nitrofurans. | five nitrofurans- nitrofurantoin, nifuratel, nitrofurazone, furazolidone and and sq 18,506--have been tested against 201 microbial strains. escherichia coli, streptococcus faecalis, micrococci and staphylococcus epidermidis were sensitive to all five compounds; furazolidone and sq 18,506 were the most active against these species. klebsiella aerogenes was less sensitive, and proteus spp., providencia stuartii and pseudomonas aeruginosa strains were resistant. nifuratel was inhibitory for some ca ... | 1978 | 657876 |
| method for the simultaneous establishment of many axenic cultures of paramecium. | a method is described for the simultaneous treatment of 42 (or more) stocks of paramecium, and their adaptation to growth in axenic culture. samples of dense cultures of these ciliates growing with enterobacter aerogenes are rendered bacteria-free by migration through 2 sets of tubes containing adaptation medium (peters' salts solution, stigmaterol, vitamins, and autoclaved e. aerogenes). the 2nd set of tubes contains adaptation medium plus antibiotics. bacteria-free samples containing approxima ... | 1978 | 660565 |
| phosphoribosylglycinamide synthetase from aerobacter aerogenes. | 1978 | 692385 | |
| enterobacter aerogenes primary bacteremia in pediatric patients. | enterobacter aerogenes bacteremia associated with the infusion of contaminated admixed intravenous (iv) fluid occurred in seven patients in a pediatric hospital over a five-day period. clinical illness was characterized by spiking fever in all patients. the temporal clustering of cases allowed for rapid recognition of the problem. the primary control measure was the prompt replacement of the iv fluids, although iv antibiotics were also administered. hospital pharmacy practices for admixing iv so ... | 1978 | 704200 |
| a sulphate metabolite of trimethoprim in goats and pigs. | 1. urine from goats and pigs after intravenous administration of [14c]trimethoprim contained an unidentified fraction of 'other water-soluble metabolites'. this fraction accounts for 56% of the administered dose in goat urine and 65% in pig urine. 2. a sulphate metabolite comprising the major part of the latter fraction in both goat and pig urine was isolated by gel filtration and paper chromatography and identified by spectroscopic methods as 2,4-diamino-5-(3,4-dimethoxy-5-sulphooxybenzyl)pyrim ... | 1978 | 726512 |
| [numerical taxonomic studies on enterobacteria of surface-water (author's transl)]. | 435 enterobacterial strains of surface-water were tested in 35 physiological characters and classified numerically by a single-linkage procedure. the relations of similarity are shown in a "vernetzungsdiagramm" (connected graph, fig. 1). tab. 1 specifies the characters of the taxa, which were formed graphically. the following facts are the result of this taxonomic classification: 1. 32% of the escherichia group are kcn-positive. in addition it was found that 60% of the e. coli isolated from the ... | 1978 | 735569 |
| [characteristics of the enterobacteria strains isolated from the urine in urinary tract infections]. | study of biochemical properties of 421 strains of enterobacteria permitted to refer them to the following taxonomic groups of which e. coli constituted 83,4 +/- 3.6%, enterobactercloacae--97 +/- 3.0%, klebsiella 4.0 +/- 2.0%. enterobacter aerogenes, enterobacter hafnia, citrobacter, proteus rettgeri, proteus vulgaris were revealed in individual cases (0.2-1.0%). as a result of serological typing there were revealed 105 serological types of escherichia differing by 55 o-antigen variants, 26 h-ant ... | 1978 | 747014 |
| carbon and nitrogen nutrition of aerobacter aerogenes for valine production. | a number of carbon and nitrogen compounds were tested for their effect on growth of aerobacter aerogenes and valine production. sucrose was found to be the superior carbon source for valine production, while mannose supported maximum cellular growth with very poor yield of valine. urea was found to be the adequate nitrogen source for valine production. supplementation of the synthetic medium with complex nutrients lowered the yield of valine. no direct relationship between the growth of the orga ... | 1978 | 749410 |
| colonization resistance against potentially pathogenic bacteria in hexaflora-associated gnotobiotic mice. | a study of colonization resistance against potentially pathogenic bacteria (escherichia coli and pseudomonas aeruginosa) was conducted in hexaflora-associated gnotobiotic mice. groups of germfree akr mice were swabbed with five bacterial and a single gastrointestinal yeast species: streptococcus faecalis. lactobacillus brevis. staphylococcus epidermidis, enterobacter aerogenes, bacteroides fragilis var. vulgatus, and torulopsis sp. all species became established in the gut in 8 weeks. later thes ... | 1978 | 417787 |
| [effect of synthetic iron chelating agensts and of a source of carbohydrate nutrition on catechol biosynthesis by klebsiella aerogenes]. | 1978 | 416325 | |
| lytic activity of a new cephalosporin, cefuroxime, against gram-negative bacteria. | cefuroxime, a new cephalosporin antibiotic, was inhibitory at low concentrations to several types of gram-negative bacteria. it was considerably more stable than cephaloridine or cephalothin to the beta-lactamases produced by escherichia coli (rp1), klebsiella aerogenes k1 and enterobacter cloacae p99. concentrations of cefuroxime much greater than the minimum inhibitory concentration (mic) were usually necessary to induce lysis of beta-lactamase and non-beta-lactamase producers. in contrast, ce ... | 1978 | 359269 |
| genetic mapping of tyramine oxidase and arylsulfatase genes and their regulation in intergeneric hybrids of enteric bacteria. | the genes for arylsulfatase (atsa) and tyramine oxidase (tyna) have been mapped in klebsiella aerogenes by p1 transduction. they are linked to gdhd and trp in the order atsa-tyna-gdhd-trp-pyrf. complementation analysis using f' episomes from escherichia coli suggested an analogous location of these genes in e. coli, although arylsulfatase activity was not detected in e. coli. p1 phage and f' episomes were used to create intergeneric hybrid strains of enteric bacteria by transfer of the ats and t ... | 1978 | 361719 |
| trypsin peptide patterns of tryptophan synthase beta2 protein among four species of the enterobacteriaceae. | the tryptophan synthase beta 2 protein (ec 4.2.1.20) of escherichia coli, enterobacter aerogenes, serratia marcescens, and erwinia carotovora was purified and compared. two-dimensional total peptide patterns for each of the four beta2 proteins obtained after digestion with trypsin showed that approximately three quarters of the total peptides are common to all four peptides. examination of only arginine-containing peptides showed that approximately half of these peptides are common. from a compa ... | 1978 | 361723 |
| aminopeptidase i activities in several microorganisms. | aminopeptidase i activity which was found to be localized in the same subcellular fraction and to be similarly heat stable was partially purified by a common procedure from escherichia coli b, escherichia coli k12, enterobacter aerogenes, salmonella typhimurium, serratia marcescens pseudomonas aeruginosa, and proteus vulgaris. the enzyme preparations were shown to contain a single animopeptidase active toward both leucylleucine and methionylalanylserine by mixed-substrate initial-velocity kineti ... | 1978 | 110425 |
| beta-lactam antibiotics. ii. structure-activity relationships of 6-[alpha-(alpha'-ureido-acylamino) acylamino] penicillanic acids. | the influence on the structure-activity relationships (s.a.r.) of the stereochemistry and various alkyl, aryl, aralkyl and heterocyclic substituents at the two chiral centres in the dipeptide side-chain of a new series of penicillins was examined. in many cases the effects of these changes had a pronounced influence on the degree of activity against gram-positive and especially gram-negative bacteria. several compounds indicated that the size, shape and spatial disposition of a substituent were ... | 1978 | 101499 |
| kinetic studies on the reactions catalyzed by chorismate mutase-prephenate dehydrogenase from aerobacter aerogenes. | steady-state kinetic techniques have been used to investigate each of the reactions catalyzed by the bifunctional enzyme, chorismate mutase-prephenate dehydrogenase, from aerobacter aerogenes. the results of steady-state velocity studies in the absence of products, as well as product and dead-end inhibition studies, suggest that the prephenate dehydrogenase reaction conforms to a rapid equilibrium random mechanism which involes the formation of two dead-end complexes, viz, enzyme-nadh-prephenate ... | 1978 | 206281 |
| electron paramagnetic resonance studies on membrane-bound respiratory nitrate reductase of klebsiella aerogenes. | 1978 | 207577 | |
| coenzyme b12-dependent diol dehydratase: regulation of apoenzyme synthesis in klebsiella pneumoniae (aerobacter aerogenes) atcc 8724. | immunochemical studies demonstrated that klebsiella pneumoniae (aerobacter aerogenes) atcc 8724 produces only a single diol dehydratase whether grown on glycerol or on 1,2-propanediol. the enzyme was subject to induction by 1,2-diols and to catabolite repression reversed by cyclic amp. | 1978 | 210157 |
| 5-methylthioribose kinase. a new enzyme involved in the formation of methionine from 5-methylthioribose. | the presence of a previously unidentified enzyme, tentatively designated 5-methylthioribose kinase, has been demonstrated in cell-free extracts of enterobacter aerogenes. the enzyme catalyzes the atp-dependent phosphorylation of 5-methylthioribose. adp is one of the products of the reaction and, based on functional group analyses, the other product is 5-methylthioribose 1-phosphate. a 40-fold purified enzyme preparation has been obtained from a cell-free extract of e. aerogenes. activity of the ... | 1978 | 210167 |
| trimethoprim-resistant klebsiella aerogenes. | 1978 | 78356 | |
| catabolic n2-acetylornithine 5-aminotransferase of klebsiella aerogenes: control of synthesis by induction, catabolite repression, and activation by glutamine synthetase. | klebsiella aerogenes formed two n2-acetylornithine 5-aminotransferases (acoat) which were separable by diethylaminoethyl-cellulose chromatography. one acoat was repressed when the cells grew on arginine-containing medium, indicating its function in arginine biosynthesis. the second acoat was induced when arginine or ornithine was present in the medium as the sole source of carbon or nitrogen, suggesting its function in the catabolism of these compounds. the induced enzyme was purified almost to ... | 1978 | 24039 |
| bacterial 2,3-butanediol dehydrogenases. | enterobacter aerogenes, aeromonas hydrophila, serratia marcescens and staphylococcus aureus possessing l(+)-butanediol dehydrogenase produced mainly meso-butanediol and small amounts of optically active butanediol; acetobacter suboxydans, bacillus polymyxa and erwinia carotovora containing d(-)-butanediol dehydrogenase produced more optically active butanediol than meso-butanediol. resting and growing cells of these organisms oxidezed only one enantiomer of racemic butanediol. the d(-)-butanedio ... | 1978 | 25056 |
| involvement of the product of the glnf gene in the autogenous regulation of glutamine synthetase formation in klebsiella aerogenes. | mutations in a site, glnf, linked by p1-mediated transduction of argg on the chromosome of klebsiella aerogenes, result in a requirement for glutamine. mutants in this gene have in all media a level of glutamine synthetase (gs) corresponding to the level found in the wild-type strain grown in the medium producing the strongest repression of gs. the adenylylation and deadenylylation of gs in glnf mutants is normal. the glutamine requirement of glnf mutants could be suppressed by mutations in the ... | 1978 | 25264 |
| glutamine synthetase of klebsiella aerogenes: properties of glnd mutants lacking uridylyltransferase. | the glnd mutation of klebsiella aerogenes is cotransducible by phage p1 with pan (requirement for pantothenate) and leads to a loss of uridylytransferase and uridylyl-removing enzyme, components of the glutamine synthetase adenylylation system. this defect results in an inability to deadenylylate glutamine synthetase rapidly and in a requirement for glutamine for normal growth. suppression of the glnd mutation are located at the glutamine synthetase structural gene glna. | 1978 | 26659 |
| effect of ph on the antimicrobial activity of some triphenylmethane dyes. | four common dyes were tested as inhibitors of four types of bacteria over the ph range 5.0-9.0. inhibition of the gram-negative types, salmonella anatum and enterobacter aerogenes, was markedly affected by the ph of the medium. these organisms tolerated concentrations of crystal violet and ethyl violet about 100-fold higher at ph 5.0 than at ph 9.0. above ph 7.0 brilliant green (bg) and malachite green (mg) were precipitated as their respective carbinols and lost their inhibitory properties with ... | 1978 | 27297 |
| in vitro antimicrobial activity of some cyclic hydroxamic acids and related lactams. | against enterobacter aerogenes 13048, serratia marcescens 13880, klebsiella pneumoniae 10031, pseudomonas aeruginosa 10145, escherichia coli 9723, lactobacillus casei 7469, lactobacillus plantarum 8014, leuconostoc dextranicum 8086, and streptococcus faecalis 8043, the mean minimal inhibitory concentrations of three cyclic hydroxamic acids, 3-amino-3,4-dihydro-1-hydroxycarbostyril, the 6-chloro analog, and the 7-chloro analog, were 0.6, 0.6, and 0.2 micrograms/ml, and those of the corresponding ... | 1978 | 400828 |
| glutamine synthetase regulation, adenylylation state, and strain specificity analyzed by polyacrylamide gel electrophoresis. | we used polyacrylamide gel electrophoresis to examine the regulation and adenylylation states of glutamine synthetases (gss) from escherichia coli (gs(e)) and klebsiella aerogenes (gs(k)). in gels containing sodium dodecyl sulfate (sds), we found that gs(k) had a mobility which differed significantly from that of gs(e). in addition, for both gs(k) and gs(e), adenylylated subunits (gs(k)-adenosine 5'-monophosphate [amp] and gs(e)-amp) had lesser mobilities in sds gels than did the corresponding n ... | 1979 | 33958 |
| enzymes of agmatine degradation and the control of their synthesis in klebsiella aerogenes. | the degradation of agmatine to succinate by klebsiella aerogenes occurs in five steps. the enzyme catalyzing the first step, agmatinase, is induced by agmatine. the enzymes catalyzing the second and third steps, putrescine aminotransferase and 4-aminobutyraldehyde dehydrogenase, are induced by putrescine and also by their product, 4-aminobutyrate. the enzymes catalyzing the fourth and fifth steps, 4-aminobutyrate aminotransferase and succinate semialdehyde dehydrogenase, are induced by 4-aminobu ... | 1979 | 35512 |
| rate of demethylation of methylmercuric chloride by enterobacter aerogenes and serratia marcescens. | 1979 | 36188 | |
| distinctive properties of glutamine synthetase from the cyanobacterium anacystis nidulans. | the intracellular levels of glutamine synthetase (gs) in anacystis nidulans grown under different conditions were determined using a whole-cell assay. nitrate-grown cells have 64% more gs than cells grown in ammonium sulfate. nitrogen starvation does not affect gs levels appreciably. incubation of nitrate-grown cells with ammonium sulfate does not change the ratio of gamma-glutamyl transferase activities stimulated by mg2+ and mn2+ ions. an in vitro test of adenylylation indicates that algae do ... | 1979 | 38892 |
| control of hospital epidemic of gentamicin-resistant klebsiella aerogenes. | 1979 | 89322 | |
| purification, properties, and kinetics of d-ribulokinase from aerobacter aerogenes. | the enzyme d-ribulokinase from aerobacter aerogenes was purified to near homogeneity. the molecular weight, as determined by sephacryl gel chromatography, is 116,000. the subunit molecular weight, determined by sodium dodecyl sulfate-gel electrophoresis, is 59,000, suggesting that d-ribulokinase is a dimer of identical subunits. initial rate kinetic studies, involving substrate analogs and products, were carried out. these investigations support a kinetic mechanism of the random bi bi type. isot ... | 1979 | 220218 |