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forced egg retention induced by diethyl-phenylacetamide diminishes the fecundity and longevity of dengue vectors.oviposition is an important event in the life cycle of mosquito which is a decisive factor for the fate of its progeny. if oviposition is prevented, the life cycle of mosquito is disrupted and the population growth can be reduced. diethyl-phenylacetamide (depa) is an effective topical repellent against various haematophagous insects. the study reports the egg retention effect and subsequent lethal effect of depa on the gravid females of aedes aegypti and ae. albopictus mosquitoes.201526714511
larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by seaweed extracts in aedes aegypti (diptera: culicidae).to investigate the larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by the extracts derived from seaweeds bryopsis pennata (b. pennata), sargassum binderi (s. binderi) and padina australis in aedes aegypti (ae. aegypti) larvae and to characterize the phytochemical components of the three seaweeds.201526706671
synthesis of silver nanoparticles using catharanthus roseus root extract and its larvicidal effects.phytosynthesis of silver nanoparticles has attracted considerable attention due to their biocompatibility, low toxicity, cost-effectiveness and being a novel method has an eco-friendly approach. biological activity of root extracts as well as synthesized silver nanoparticles of catharanthus roseus were evaluated against larvae of aedes aegyptiand culex quinquefasciatus. the structure and proportion of the synthesized nanoparticles was defined by exploitation ultraviolet spectrophotometry, x-ray ...201526688962
effect of isodillapiole on the expression of the insecticide resistance genes gste7 and cyp6n12 in aedes aegypti from central amazonia.the yellow fever mosquito aedes (stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. dengue prevention measures for the control of a. aegypti involve mainly the use of synthetic insecticides. the constant use of insecticides has caused resistance in this mosquito. alternative studies on plant extracts and their products have been conducted with the aim of controlling the spread of the mosquito. dillapiole is a compound found in essential oils of the plant piper adunc ...201526681019
aedes aegypti eggs oviposited on water surface collected from field ovitraps in nova iguaçu city, brazil.aedes aegypti eggs can be collected from the water surface.201526676505
suitability of mixing fluorescent dye in adulticides and its impact on droplet characteristics and pesticide efficacy.fluorescent dyes are commonly used to help visualize insecticidal droplets or to trace movement of insecticides; however, the effect these dyes have on the insecticide's efficacy and droplet characteristics is unknown. this study evaluated the effects of mixing uvitex ob fluorescent dye with 5 adulticides on their efficacy in a wind tunnel. efficacy was determined via droplet size characteristics, spray flux (active ingredient [ai] deposition), and female adult aedes aegypti mortality. fyfanon® ...201526675457
interspecific cross-mating between aedes aegypti and aedes albopictus laboratory strains: implication of population density on mating behaviors.mating behavior between aedes aegypti and ae. albopictus, established colony strains were examined under laboratory conditions (30-cm(3) screened cages) for 5 consecutive days. the effect of selected male densities (30, 20, 10) and female density (20) on the number of swarming, mating pairs, eggs produced, and inseminated females were evaluated. male densities significantly increased swarming behavior, mating pairs, and egg production of heterospecific females, but female insemination was reduce ...201526675452
bioactive compound synthesis of ag nanoparticles from leaves of melia azedarach and its control for mosquito larvae.larvicidal activity of synthesized ag nanoparticles using 2,7.bis[2-[diethylamino]-ethoxy]fluorence isolate from the melia azedarach leaves against aedes aegypti and culex quinquefasciatus. six fractions were collected and concentrated, fraction three showed a single spot on tlc which was found to be a pure compound. the structures were elucidated by analyses of uv, ms, and nmr spectral data. the maximum mortality was fluorence against a. aegypti and c. quinquefasciatus (lc50 = 7.94, lc90 = 23.8 ...201525496834
chemical composition and biological activity of four salvia essential oils and individual compounds against two species of mosquitoes.the chemical compositions of essential oils obtained from four species of genus salvia were analyzed by gas chromatography with a flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms). the main compounds identified from salvia species essential oils were as follows: 1,8-cineole (71.7%), α-pinene (5.1%), camphor (4.4%), and β-pinene (3.8%) in salvia apiana; borneol (17.4%), β-eudesmol (10.4%), bornyl acetate (5%), and guaiol (4.8%) in salvia elegans; bornyl acetate ( ...201525531412
effect of confertifolin from polygonum hydropiper l. against dengue vector mosquitoes aedes aegypti l.the essential oil from the leaves of polygonum hydropiper l. (polygonaceae) was tested against aedes aegypti l. the lc50 values were 190.72 and 234.37 ppm against second and fourth instar larvae of a. aegypti, respectively. confertifolin (6,6,9a-trimethy l-4,5,5a,6,7,8,9,9a-octahydronaphtho [1,2-c] furan-3 (1h)-one) was isolated from the essential oil of p. hydropiper leaves using silica gel column chromatography. the lc50 values were 2.90 and 2.96 ppm for second and fourth instar larvae of a. a ...201525523289
são paulo urban heat islands have a higher incidence of dengue than other urban areas.urban heat islands are characterized by high land surface temperature, low humidity, and poor vegetation, and considered to favor the transmission of the mosquito-borne dengue fever that is transmitted by the aedes aegypti mosquito. we analyzed the recorded dengue incidence in sao paulo city, brazil, in 2010-2011, in terms of multiple environmental and socioeconomic variables. geographical information systems, thermal remote sensing images, and census data were used to classify city areas accord ...201525523076
mukia maderaspatana (cucurbitaceae) extract-mediated synthesis of silver nanoparticles to control culex quinquefasciatus and aedes aegypti (diptera: culicidae).mosquitoes and mosquito-borne diseases are prone to raise health and economic impacts. synthetic insecticide-based interventions are indeed in situations of epidemic outbreak and sudden increases of adult mosquitoes. nanoparticles are being used in many commercial applications and were found that aqueous silver ions can be reduced by an aqueous extract of plant parts to generate extremely stable silver nanoparticles in water. based on this, silver nanoparticles (snps) were synthesized using leaf ...201525601441
insecticidal and repellent activity of clausena dentata (rutaceae) plant extracts against aedes aegypti and culex quinquefasciatus mosquitoes (diptera: culicidae).mosquito control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of botanical origin may serve as suitable alternative biocontrol agents. the present study is to evaluate adulticidal activity of clausena dentata plant extract against aedes aegypti and culex quinquefasciatus mosquitoes. the adult mortality was observed after 24 h of exposure. the highest mortality was found in acetone extracts against ae. aegypti and cx. quinquefasciatus with the lc50 ...201525573693
hptlc analysis of scoparia dulcis linn (scrophulariaceae) and its larvicidal potential against dengue vector aedes aegypti.this study evaluates the larvicidal activity of scoparia dulcis aqueous extract against dengue vector and determines its major chemical components. the extract was tested at various concentrations ranging from 0.1 to 2 mg/ml against aedes aegypti larvae. the extracts displayed significant larvicidal efficacy against ae. aegypt species after 24 h exposure revealing lc50 of 3.3835 (mg/ml) and lc90 of 5.7578 (mg/ml). finger printing profile carried out by camag automatic tlc sample applicator progr ...201525573588
aealrack1 expression and localization in response to stress in c6/36 ht mosquito cells.the receptor for activated c kinase 1 (rack1), a scaffold protein member of the tryptophan-aspartate (wd) repeat family, folds in a seven-bladed β-propeller structure that permits the association of proteins to form active complexes. mosquitoes of the genus aedes sp., are vectors of virus producing important diseases such as: dengue, chikungunya and yellow fever. based on the highly conserved gene sequence of aeaerack1 of the mosquito aedes aegypti we characterized the mrna and protein of the ho ...201525555378
evaluation of some aromatic plant extracts for mosquito larvicidal potential against culex quinquefasciatus, aedes aegypti, and anopheles stephensi.in the present investigation, larvicidal potential of hexane, choloroform, ethyl acetate, acetone, and methanol extracts of seven aromatic plants, viz., blumea mollis, chloroxylon swietenia, clausena anisata, feronia limnonia, lantana camera, plectranthus amboinicus, and tagetes erecta were screened against culex quinquefasciatus, aedes aegypti, and anopheles stephensi. the larval mortality was observed after 12 and 24 h of exposure period. the results revealed that all the extracts showed varie ...201525630696
dengue in brazil and colombia: a study of knowledge, attitudes, and practices.introduction this study was conducted in brazil and colombia,where dengue is endemic and vector control programs use chemical insecticides. methods we identified knowledge, attitudes, and practices about dengue and determined the infestation levels of aedes aegypti in one brazilian and four colombian communities. results the surveys show knowledge of the vector, but little knowledge about diagnosis, prognosis, and treatment. vector infestation indices show brazil to have good relative control, w ...201525626660
immature aedes mosquitoes colonize culex quinquefasciatus breeding sites in neighborhoods in the municipality of olinda, state of pernambuco.the present study shows the colonization of aedes mosquitoes in breeding sites specific for culex quinquefasciatus in neighborhoods in the municipality of olinda.201525626658
angelica sinensis (umbelliferae) with proven repellent properties against aedes aegypti, the primary dengue fever vector in thailand.botanical resources with great diversity in medicinal and aromatic plants are a rich and reliable source for finding insect repellents of plant origin, which are widely popular among today's consumers. although some herbal-based repellents have been proven comparable to or even better than synthetics, commercially available natural repellents generally tend to be expensive, with short-lived effectiveness. this critical flaw leads to ongoing research for new and effective repellents, which provid ...201525773182
aedes aegypti salivary gland extract ameliorates experimental inflammatory bowel disease.current therapies for inflammatory bowel disease (ibd) are not totally effective, resulting in persistent and recurrent disease for many patients. mosquito saliva contains immunomodulatory molecules and therein could represent a novel therapy for ibd. here, we demonstrated the therapeutic activity of salivary gland extract (sge) of aedes aegypti on dextran sulfate sodium (dss)-induced colitis. for this purpose, c57bl/6 male mice were exposed to 3% dss in drinking water and treated with sge at ea ...201525770821
cytostatic and genotoxic effect of temephos in human lymphocytes and hepg2 cells.temephos is an organophosphorus pesticide that is used in control campaigns against aedes aegypti mosquitoes, which transmit dengue. in spite of the widespread use of temephos, few studies have examined its genotoxic potential. the aim of this study was to evaluate the cytotoxic, cytostatic and genotoxic effects of temephos in human lymphocytes and hepatoma cells (hepg2). the cytotoxicity was evaluated with simultaneous staining (fda/etbr). the cytostatic and genotoxic effects were evaluated usi ...201525746384
rnai knockdown of bmrab3 led to larva and pupa lethality in silkworm bombyx mori l.rab3 gtpases are known to play key a role in vesicular trafficking, and express highest in brain and endocrine tissues. in mammals, rab3 gtpases are paralogs unlike in insect. in this study, we cloned rab3 from the silk gland tissue of silkworm bombyx mori, and identified it as bmrab3. our in silico analysis indicated that bmrab3 is an isoform with a theoretical isoelectric point and molecular weight of 5.52 and 24.3 kda, respectively. further, bmrab3 showed the c-terminal hypervariability for g ...201525735242
effect of temperature on life history traits during immature development of aedes aegypti and culex quinquefasciatus (diptera: culicidae) from córdoba city, argentina.we investigated how ambient temperature under fluctuating conditions affects the larval-pupal immature traits of aedes aegypti and culex quinquefasciatus mosquitoes from córdoba city, argentina, and established each species development threshold and physiological time. based on life tables, three cohorts of each mosquito species were reared in the laboratory under small fluctuating temperatures conditions of 15.2±1.7°c, 17.9±1.6°c, 21.6±0.7°c and 25.3±0.4°c for ae. aegypti, and 16.6±1.7°c, 18.7± ...201525733491
identification of chemical constituents and larvicidal activity of essential oil from murraya exotica l. (rutaceae) against aedes aegypti, anopheles stephensi and culex quinquefasciatus (diptera: culicidae).this study was conducted to evaluate the phytochemical composition and larvicidal effect of leaf essential oil from murraya exotica against early fourth-instar larvae of aedes aegypti, anopheles stephensi and culex quinquefasciatus. gas chromatography (gc) and gas chromatography mass spectrometry (gc-ms) analyses revealed that the essential oil contained 27 components. the major chemical components identified were β-humulene (40.62%), benzyl benzoate (23.96%), β-caryophyllene (7.05%) and α-terpi ...201525697880
tackling the growing threat of dengue: phyllanthus niruri-mediated synthesis of silver nanoparticles and their mosquitocidal properties against the dengue vector aedes aegypti (diptera: culicidae).mosquitoes are vectors of devastating pathogens and parasites, causing millions of deaths every year. dengue is a mosquito-borne viral infection found in tropical and subtropical regions around the world. recently, transmission has strongly increased in urban and semiurban areas, becoming a major international public health concern. aedes aegypti (diptera: culicidae) is the primary vector of dengue. the use of synthetic insecticides to control aedes mosquitoes lead to high operational costs and ...201525669140
synthesis and characterization of silver nanoparticles using gmelina asiatica leaf extract against filariasis, dengue, and malaria vector mosquitoes.mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. the continued use of synthetic insecticides has resulted in resistance in mosquitoes. synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternative to synthetic insecticides because they are effective, biodegradable, eco-friendly, and safe to envir ...201525666372
an in vitro bioassay for the quantitative evaluation of mosquito repellents against stegomyia aegypti (=aedes aegypti) mosquitoes using a novel cocktail meal.to assess the efficacy of new insect repellents, an efficient and safe in vitro bioassay system using a multiple-membrane blood-feeding device and a cocktail meal was developed. the multiple-membrane blood-feeding device facilitates the identification of new insect repellents by the high-throughput screening of candidate chemicals. a cocktail meal was developed as a replacement for blood for feeding females of stegomyia aegypti (=aedes aegypti) (l.) (diptera: culicidae). the cocktail meal consis ...201525828787
cymbopogon citratus-synthesized gold nanoparticles boost the predation efficiency of copepod mesocyclops aspericornis against malaria and dengue mosquitoes.plant-borne compounds can be employed to synthesize mosquitocidal nanoparticles that are effective at low doses. however, how they affect the activity of mosquito predators in the aquatic environment is unknown. in this study, we synthesized gold nanoparticles (aun) using the leaf extract of cymbopogon citratus, which acted as a reducing and capping agent. aun were characterized by a variety of biophysical methods and sorted for size in order to confirm structural integrity. c. citratus extract ...201525819295
extract of bowdichia virgilioides and maackiain as larvicidal agent against aedes aegypti mosquito.the larvicidal activities of extracts of three hardwood species (hymenaea stigonorcapa, anadenanthera colubrina and bowdichia virgilioides) against 4th instar larvae of aedes aegypti were evaluated using who guidelines. extracts of h. stignocarpa and a. colubrina showed weak activity. the highest larvicidal effect was obtained with the cyclohexane extract of the heartwood of b. virgilioides, which caused 100% mortality at concentrations at 50 and 100 µg/ml. fraction toluene/etoac (8:2) from this ...201525819294
effect of salinity on the behavior of aedes aegypti populations from the coast and plateau of southeastern brazil.mosquito larvae can develop in fresh, salty and brackish water. the larvae of aedes aegypti develop in fresh water. however, in laboratory studies, tolerance of this species for oviposition and hatching in brackish water was observed. immature forms of ae. aegypti have also been found developing in brackish water in coastal areas. the purpose of the study was to compare the effects of salt on the biological cycle of ae. aegypti populations from coastal and plateau areas of southeastern brazil.201525815871
[assessment of the risk of expansion of the habitats of the mosquitoes aedes aegypti l. and aedes albopictus skus. in russia].based on climatic characteristics, the authors show that an invasive aedes albopictus species is likely to emerge in new areas of the russian federation.201525812400
an entomological review of invasive mosquitoes in europe.among the invasive mosquitoes registered all over the world, aedes species are particularly frequent and important. as several of them are potential vectors of disease, they present significant health concerns for 21st century europe. five species have established in mainland europe, with two (aedes albopictus and aedes japonicus) becoming widespread and two (ae. albopictus and aedes aegypti) implicated in disease transmission to humans in europe. the routes of importation and spread are often e ...201525804287
low efficacy of delthamethrin-treated net against singapore aedes aegypti is associated with kdr-type resistance.there has been a worldwide surge in the number and severity of dengue in the past decades. in singapore, relentless vector control efforts have been put in to control the disease since the 1960's. space spraying, fogging, chemical treatment and source reduction are some commonly used methodologies for controlling its vectors, particularly aedes aegypti. here, as we explored the use of a commercially available delthamethrin-treated net as an alternative strategy and the efficacy of the treated ne ...201525801264
an investigation on the diversity of mosquitocidal bacteria and its relationship with incidence of vector borne diseases.control of mosquitoes is the most important aspect of public health, as mosquitoes transmit many human diseases, including the fatal infection, japanese encephalitis. this paper addresses the isolation of new mosquitocidal bacteria from soil samples in the union territory of pondicherry, india, where, no clinical cases of vector borne infections have been reported. bacterial isolates from soil samples were screened for potential mosquitocidal strains and bioassays against mosquito vectors (culex ...201525801257
evaluation of sumithion l-40 against aedes aegypti (l.) and aedes albopictus skuse.space spraying of chemical insecticides is still an important mean of controlling aedes mosquitoes and dengue transmission. for this purpose, the bioefficacy of space-sprayed chemical insecticide should be evaluated from time to time. a simulation field trial was conducted outdoor in an open field and indoor in unoccupied flat units in kuala lumpur, to evaluate the adulticidal and larvicidal effects of sumithion l-40, a ulv formulation of fenitrothion. a thermal fogger with a discharge rate of 2 ...201525801256
feeding stage, species, body part and sex-specific activity of glutathione s-transferase in mosquito.in the present study, the feeding stage, body parts, development and sex specific activity of glutathione s-transferases (gsts) were observed in different mosquito species (aedes aegypti, culex quinquefasciatus, anopheles stephensi, an. culicifacies, an. annularis, an. subpictus, an. vagus). gst activity was assayed spectrophotometrically at 23°c, using a uv max microplate reader, to measure the rate of conjugation of gsh to cdnb. a significant species-specific difference in the activity of gst ...201525801255
surveillance of aedes albopictus skuse breeding preference in selected dengue outbreak localities, peninsular malaysia.entomological surveillance was conducted in order to determine the abundance and to evaluate any changes of biological vectors or ecology, especially in the dengue outbreak areas. the abundance and breeding preference of aedes albopictus and aedes aegypti were conducted in selected dengue outbreak localities in three states of peninsular malaysia namely selangor, federal territory of kuala lumpur, and penang island using ovitraps and larval survey method. it was determined that ae. albopictus wa ...201525801254
nectar protein content and attractiveness to aedes aegypti and culex pipiens in plants with nectar/insect associations.we chose five easily propagated garden plants previously shown to be attractive to mosquitoes, ants or other insects and tested them for attractiveness to culex pipiens and aedes aegypti. long term imbibition was tested by survival on each plant species. both mosquito species survived best on impatiens walleriana, the common garden impatiens, followed by asclepias curassavica, campsis radicans and passiflora edulis, which sponsored survival as well as the 10% sucrose control. immediate preferenc ...201525792420
proteomic changes occurring in the malaria mosquitoes anopheles gambiae and anopheles stephensi during aging.the age of mosquitoes is a crucial determinant of their ability to transmit pathogens and their resistance to insecticides. we investigated changes to the abundance of proteins found in heads and thoraces of the malaria mosquitoes anopheles gambiae and anopheles stephensi as they aged. protein expression changes were assessed using two-dimensional difference gel electrophoresis and the identity of differentially expressed proteins was determined by using either matrix-assisted laser desorption i ...201526100052
characterization and bioassay for larvicidal activity of anacardium occidentale (cashew) shell waste fractions against dengue vector aedes aegypti.recent studies regarding the harmful effects of synthetic larvicides initiated the need to investigate for unconventional measures that are environmentally safe and target-specific against aedes aegypti larvae. thus, the main objectives of the study are to evaluate the larvicidal toxicity of the solvent fractions of anacardium occidentale shell wastes against the third and fourth instar larvae of a. aegypti and to compare the results with the commercial larvicide product. the shell wastes were e ...201526099240
surveillance of adult aedes mosquitoes in selangor, malaysia.to determine the effectiveness of using sticky traps and the ns1 dengue antigen kit for the surveillance of aedes mosquitoes for dengue control.201526094839
predation by asian bullfrog tadpoles, hoplobatrachus tigerinus, against the dengue vector, aedes aegypti, in an aquatic environment treated with mosquitocidal nanoparticles.aedes aegypti is a primary vector of dengue and chikungunya. the use of synthetic insecticides to control aedes populations often leads to high operational costs and adverse non-target effects. botanical extracts have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles, but their impact against predators of mosquito larvae has not been well studied. we propose a single-step method for the biosynthesis of silver nanoparticles (agnp) using the extract of artemisia vulgar ...201526091763
phytochemical profile and larvicidal properties of seed essential oil from nigella sativa l. (ranunculaceae), against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).the present study deals with investigation of larvicidal activity and their chemical constituents of the essential oil from the seeds of nigella sativa l. (ranunculaceae). totally, 18 chemical compounds were identified by gc and gc-ms analysis. thymol (19.13 %) and α-phellandrene (14.9 %) were identified as major chemical components followed by camphor (12.14 %), borneol (11.31 %), and carvacrol (8.65 %). the larval mortality was observed after 12 and 24 h of exposure period. the results reveale ...201526091760
thiosemicarbazones as aedes aegypti larvicidal.a set of aryl- and phenoxymethyl-(thio)semicarbazones were synthetized, characterized and biologically evaluated against the larvae of aedes aegypti (a. aegypti), the vector responsible for diseases like dengue and yellow fever. (q)sar studies were useful for predicting the activities of the compounds not included to create the qsar model as well as to predict the features of a new compound with improved activity. docking studies corroborated experimental evidence of aescp-2 as a potential targe ...201526087027
a sodium channel variant in aedes aegypti as a candidate pathogen sensor for viral-associated molecular patterns.recent work demonstrated that a splice variant of a human macrophage voltage-gated sodium channel expressed on endosomes acts as an intracellular sensor for dsrna, a viral-associated molecular pattern. here our goal was to identify a candidate gene in a clinically relevant invertebrate model with related cellular and pattern recognition properties. the para gene in drosophila and other insects encodes voltage-gated sodium channels with similar electrophysiological properties to those found in ve ...201526086103
localization and role of inward rectifier k(+) channels in malpighian tubules of the yellow fever mosquito aedes aegypti.malpighian tubules of adult female yellow fever mosquitoes aedes aegypti express three inward rectifier k(+) (kir) channel subunits: aekir1, aekir2b and aekir3. here we 1) elucidate the cellular and membrane localization of these three channels in the malpighian tubules, and 2) characterize the effects of small molecule inhibitors of aekir1 and aekir2b channels (vu compounds) on the transepithelial secretion of fluid and electrolytes and the electrophysiology of isolated malpighian tubules. usin ...201526079629
proteomic analysis of immunogenic proteins from salivary glands of aedes aegypti.humans develop anti-salivary proteins after arthropod bites or exposure to insect salivary proteins. this reaction indicates that vector bites have a positive effect on the host immune response, which can be used as epidemiological markers of exposure to the vector. our previous study identified two immunogenic proteins with molecular weights of 31 kda and 56 kda from salivary gland extract (sge) of aedes aegypti that cross-reacted with serum samples from dengue hemorrhagic fever (dhf) patients ...201526054892
the effect of photoperiod on life history and blood-feeding activity in aedes albopictus and aedes aegypti (diptera: culicidae).several studies have examined how climatic variables such as temperature and precipitation may affect life history traits in mosquitoes that are important to disease transmission. despite its importance as a seasonal cue in nature, studies investigating the influence of photoperiod on such traits are relatively few. this study aims to investigate how photoperiod alters life history traits, survival, and blood-feeding activity in aedes albopictus (skuse) and aedes aegypti (linnaeus). we performed ...201526047197
lethal ovitrap deployment for aedes aegypti control: potential implications for non-target organisms.in australia, dengue control combines source reduction with lethal ovitraps to reduce aedes aegypti populations during outbreaks. lethal ovitraps are considered a sustainable and environmentally friendly method of controlling container-inhabiting mosquitoes, however, to-date, this claim has not been quantified. this study assesses the potential impact of lethal ovitraps on non-target organisms when used to control ae. aegypti in tropical australia. for retention of specimens, we substituted stan ...201526047194
volatile semiochemical-conditioned attraction of the male yellow fever mosquito, aedes aegypti, to human hosts.we investigated the olfactory responses of male mosquitoes to kairomones of vertebrate hosts in a dual-port olfactometer. the behavioral responses of unmated and mated male and female mosquitoes from one to ten days old to human odors were compared to the odors of different human hosts. to evaluate the relationship between the age of male mosquitoes and their responses, we performed experiments with males at different ages. unmated ae. aegypti males, one to two days old, did not fly upwind to hu ...201526047178
effects of α,β-unsaturated lactones on larval survival and gut trypsin as well as oviposition response of aedes aegypti.lactones are organic cyclic esters that have been described as larvicides against aedes aegypti and as components of oviposition pheromone of culex quinquefasciatus. this work describes the effect of six α,β-unsaturated lactones (5a-5f) on survival of a. aegypti fourth instar larvae (l4). it is also reported the effects of the lactones on l4 gut trypsin activity and oviposition behavior of a. aegypti females. five lactones were able to kill l4 being the lactones 5a (lc50 of 39.05 ppm), 5e (lc50 ...201526044355
spatial models for prediction and early warning of aedes aegypti proliferation from data on climate change and variability in cuba.climate variability, the primary expression of climate change, is one of the most important environmental problems affecting human health, particularly vector-borne diseases. despite research efforts worldwide, there are few studies addressing the use of information on climate variability for prevention and early warning of vector-borne infectious diseases.201526027583
[omics of vector mosquitoes: a big data platform for vector biology and vector-borne diseases].recently the studies on mosquito genomics, transcriptomics and small rnaomics developed rapidly with the novel biotechnologies of the next generation sequencing techniques. the genome sequences of several important vector mosquitoes including anopheles gambiae, culex quinquefasciatus, and aedes aegypti have been published. the genome sizes vary among the different species of mosquitoes and are consistent with the number of the repeat regions. the released genome sequences facilitate gene cloning ...201526018253
annona muricata leaf extract-mediated silver nanoparticles synthesis and its larvicidal potential against dengue, malaria and filariasis vector.mosquitoes transmit several diseases which cause millions of deaths every year. the use of synthetic insecticides to control mosquitoes caused diverse effects to the environment, mammals, and high manufacturing cost. the present study was aimed to test the larvicidal activity of green synthesized silver nanoparticles using annona muricata plant leaf extract against third instar larvae of three medically important mosquitoes, i.e., aedes aegypti, anopheles stephensi, and culex quinquefasciatus. t ...201526002825
unique biochemical and molecular biological mechanism of synergistic actions of formamidine compounds on selected pyrethroid and neonicotinoid insecticides on the fourth instar larvae of aedes aegypti (diptera: culicidae).we recently reported that formamidine pesticides such as amitraz and chlordimeform effectively synergize toxic actions of certain pyrethroid and neonicotinoid insecticides in some insect species on the 4th instar larvae of aedes aegypti. here we studied the biochemical basis of the synergistic actions of the formamidines in amplifying the toxicity of neonicotinoids and pyrethroids such as dinotefuran and thiamethoxam, as well as deltamethrin-fenvalerate type of pyrethroids. we tested the hypothe ...201525987221
synergistic action of octopamine receptor agonists on the activity of selected novel insecticides for control of dengue vector aedes aegypti (diptera: culicidae) mosquito.studying insecticide resistance in mosquitoes has attracted the attention of many scientists to elucidate the pathways of resistance development and to design novel strategies in order to prevent or minimize the spread and evolution of resistance. here, we tested the synergistic action of piperonyl butoxide (pbo) and two octopamine receptor (or) agonists, amitraz (amz) and chlordimeform (cdm) on selected novel insecticides to increase their lethal action on the fourth instar larvae of aedes aegy ...201525987220
experience- and age-mediated oviposition behaviour in the yellow fever mosquito stegomyia aegypti (=aedes aegypti).in repeated behaviours such as those of feeding and reproduction, past experiences can inform future behaviour. by altering their behaviour in response to environmental stimuli, insects in highly variable landscapes can tailor their behaviour to their particular environment. in particular, female mosquitoes may benefit from plasticity in their choice of egg-laying site as these sites are often temporally variable and clustered. the opportunity to adapt egg-laying behaviour to past experience als ...201525982411
larvicidal activity against aedes aegypti of foeniculum vulgare essential oils from portugal and cape verde.dengue is a potentially fatal mosquito-borne infection with 50 million cases per year and 2.5 billion people vulnerable to the disease. this major public health problem has recurrent epidemics in latin america and occurred recently in cape verde and madeira island. the lack of anti-viral treatment or vaccine makes the control of mosquito vectors a high option to prevent virus transmission. essential oil (eo) constituents can affect insect's behaviour, being potentially effective in pest control. ...201525973508
imidacloprid impairs the post-embryonic development of the midgut in the yellow fever mosquito stegomyia aegypti (=aedes aegypti).the mosquito stegomyia aegypti (=aedes aegypti) (diptera: culicidae) is a vector for the dengue and yellow fever viruses. as blood digestion occurs in the midgut, this organ constitutes the route of entry of many pathogens. the effects of the insecticide imidacloprid on the survival of st. aegypti were investigated and the sub-lethal effects of the insecticide on midgut development were determined. third instar larvae were exposed to different concentrations of imidacloprid (0.15, 1.5, 3.0, 6.0 ...201525968596
oxime derivatives with larvicidal activity against aedes aegypti l.oximes containing secondary metabolites constitute an important group of bioactive compounds and have been described and frequently updated in the literature due to their pharmacological properties. thus, the aim of this study was to evaluate the larvicidal activity of a series of fourteen structurally related [1,4]-benzoquinone mono-oximes on third-instar aedes aegypti larvae and to investigate structure-activity relationships (sar) of these compounds. results of larvicidal assay revealed that ...201525956398
involvement of metabolic resistance and f1534c kdr mutation in the pyrethroid resistance mechanisms of aedes aegypti in india.pesticide resistance poses a serious problem for worldwide mosquito control programs. resistance to insecticides can be caused by an increased metabolic detoxification of the insecticide and/or by target site insensitivity. in the present study, we estimated the tolerance of indian aedes aegypti populations using adult bioassays that revealed high resistance levels of the field populations to permethrin (rr-6, 5.8 and 5.1 folds) compared to our susceptible population. enzymatic assays revealed i ...201525944353
susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (diptera: culicidae) from muang district, phitsanulok province, thailand.to investigate the susceptibility to temephos, permethrin and deltamethrin of aedes aegypti (ae. aegypti), collected from areas with high incidence of dengue hemorrhagic fever cases in phitsanulok province, thailand.201525901918
frizzled 2 is a key component in the regulation of tor signaling-mediated egg production in the mosquito aedes aegypti.the wnt signaling pathway was first discovered as a key event in embryonic development and cell polarity in drosophila. recently, several reports have shown that wnt stimulates translation and cell growth by activating the mtor pathway in mammals. previous studies have demonstrated that the target of rapamycin (tor) pathway plays an important role in mosquito vitellogenesis. however, the interactions between these two pathways are poorly understood in the mosquito. in this study, we hypothesized ...201525890109
structure-activity relationship (sar) modelling of mosquito larvicides.an attempt was made to derive structure-activity models allowing the prediction of the larvicidal activity of structurally diverse chemicals against mosquitoes. a database of 188 chemicals with their activity on aedes aegypti larvae was constituted from analysis of original publications. the activity values were expressed in log 1/ic50 (concentration required to produce 50% inhibition of larval development, mmol). all the chemicals were encoded by means of codessa and autocorrelation descriptors ...201525864415
territorial analysis of aedes aegypti distribution in two colombian cities: a chorematic and ecosystem approach.a territorial analysis of aedes aegypti density was conducted in two colombian cities using an ecosystem and chorematic approach. entomological and behavioral data (by cluster) and information on the urban context were used to analyze the relationship between territorial structures and dynamics and vector density. the results were represented in graphic (chorematic) models. arauca showed higher vector density than armenia. higher density was related to unplanned urbanization, flood-prone areas, ...201525859719
gc-ms analysis of bioactive components and synthesis of gold nanoparticle using chloroxylon swietenia dc leaf extract and its larvicidal activity.a rapid bio-reduction of chloroauric acid (haucl4) was achieved by chloroxylon swietenia dc leaf extract (csle), which resulted in the formation of well dispersed c. swietenia gold nanoparticles (csgnps). the formation of gnps was confirmed by color changes from yellowish green to purple and their characteristic peak at 545 nm. the characterization of synthesized csgnps was made through x-ray diffraction (xrd), fourier transform infrared spectroscopy (ftir), high resolution transmission electron ...201525854160
evaluation of the atraedes™ lure for collection of culex quinquefasciatus in gravid traps.the typical attractant used in gravid trapping of culex quinquefasciatus is an aged infusion of organic materials, which can change in attractiveness over time. a standardized chemical attractant dispenser derived from grass infusion, the atraedes™ lure, has been produced for the surveillance of the dengue vector aedes aegypti. a study using this lure in combination with the centers for disease control and prevention gravid traps was conducted in tanga, tanzania. the addition of the lure to trap ...201525843185
evaluation of a new spraying machine for barrier treatment and penetration of bifenthrin on vegetation against mosquitoes.the effectiveness and penetration of a novel, truck-mounted mist sprayer (3wc-30-4p provided by american longray) was evaluated with bifenthrin in a large, park-like setting with historic floodwater and woodland mosquito populations. efficacy evaluations were determined through adult population collections and excised leaf bioassays. trapping results showed a mean reduction of 77% in mosquito populations in the treated area for 5 sampling events up to 4 wk posttreatment. leaf bioassays revealed ...201525843180
larvicidal activity of naturally occurring naphthalenedione and its structurally related analogs against three mosquito species.the objective of this study was to determine the larvicidal activity of an active compound isolated from cercis chinensis heartwood and its structurally related analogs against 4th-stage aedes aegypti, culex pipiens pallens, and ae. togoi. the larvicidal compound of c. chinensis was isolated with the use of various chromatographic techniques and identified as analogs of 1,4-naphthalenedione. based on the median lethal concentration (lc(50)) values of commercially procured analogs against ae. aeg ...201525843178
laboratory studies of aedes aegypti attraction to ketones, sulfides, and primary chloroalkanes tested alone and in combination with l-lactic acid.the attraction of female aedes aegypti to single compounds and binary compositions containing l-lactic acid and an additional saturated compound from a set of ketones, sulfides, and chloroalkanes was studied using a triple-cage dual-port olfactometer. these chemical classes were studied because of their structural relation to acetone, dimethyl disulfide, and dichloromethane, which have all been reported to synergize attraction to l-lactic acid. human odors, carbon dioxide, and the binary mixture ...201525843177
two novel bioassays to assess the effects of pyrethroid-treated netting on knockdown-susceptible versus resistant strains of aedes aegypti.we describe 2 new mosquito bioassays for use with insecticide-treated netting or other textiles. the 1st is a cylinder bioassay in which a mosquito is forced to contact treated material regardless of where it lands within the bioassay construct. the 2nd is a repellency/irritancy and biting-inhibition bioassay (ribb) in which human arms and breath are used as attractants. mosquitoes have the choice to pass through holes cut in untreated or treated netting to move from a center release chamber int ...201525843176
comparison of bg-sentinel® trap and oviposition cups for aedes aegypti and aedes albopictus surveillance in jacksonville, florida, usa.the bg-sentinel® (bgs) trap and oviposition cups (ocs) have both proven effective in the surveillance of aedes species. this study aimed to determine which of the 2 traps could best characterize the relative population sizes of aedes albopictus and aedes aegypti in an urban section of jacksonville, fl. until 1986, ae. aegypti was considered the dominant container-breeding species in urban northeastern florida. since the introduction of ae. albopictus, ae. aegypti has become almost completely ext ...201525843173
eco-friendly control of malaria and arbovirus vectors using the mosquitofish gambusia affinis and ultra-low dosages of mimusops elengi-synthesized silver nanoparticles: towards an integrative approach?mosquito-borne diseases represent a deadly threat for millions of people worldwide. however, the use of synthetic insecticides to control culicidae may lead to high operational costs and adverse non-target effects. plant-borne compounds have been proposed for rapid extracellular synthesis of mosquitocidal nanoparticles. their impact against biological control agents of mosquito larval populations has been poorly studied. we synthesized silver nanoparticles (agnp) using the aqueous leaf extract o ...201526300364
further evidences for the mode of action of the larvicidal m-pentadecadienyl-phenol isolated from myracrodruon urundeuva seeds against aedes aegypti.nowadays, dengue fever is considered the most important arbovirosis worldwide and its control is still based upon combating the vector aedes aegypti. besides monitoring of mosquito populations resistant to conventional insecticides, the search for new environmentally safe insecticides and conduction of molecular studies focusing on the elucidation of mode of action and possible resistance mechanisms are considered the key for a sustainable management of the mosquito vector. thus, the present wor ...201526299195
widespread evidence for interspecific mating between aedes aegypti and aedes albopictus (diptera: culicidae) in nature.aedes aegypti and aedes albopictus, two important vectors of the dengue and chikungunya viruses to humans, often come in contact in their invasive ranges. in these circumstances, a number of factors are thought to influence their population dynamics, including resource competition among the larval stages, prevailing environmental conditions and reproductive interference in the form of satyrization. as the distribution and abundance of ae. aegypti and ae. albopictus have profound epidemiological ...201526296606
trace samples of human blood in mosquitoes as a forensic investigation tool.investigations of any type of crime invariably starts at the crime scene by collecting evidence. thus, the purpose of this research was to collect and analyze an entomological trace from an environment that is similar to those of indoor crime scenes. hematophagous mosquitoes were collected from two residential units; saliva of volunteers that were residents in the units was also collected for genetic analysis as reference samples. we examined the allele frequencies of 15 short tandem repeat loci ...201526600546
chitosan, carbon quantum dot, and silica nanoparticle mediated dsrna delivery for gene silencing in aedes aegypti: a comparative analysis.in spite of devastating impact of mosquito borne pathogens on humans, widespread resistance to chemical insecticides and environmental concerns from residual toxicity limit mosquito control strategies. we tested three nanoparticles, chitosan, carbon quantum dot (cqd), and silica complexed with dsrna, to target two mosquito genes (snf7 and src) for controlling aedes aegypti larvae. relative mrna levels were quantified using qrt-pcr to evaluate knockdown efficiency in nanoparticle-dsrna treated la ...201526291176
an environmentally safe larvicide against aedes aegypti based on in situ gelling nanostructured surfactant systems containing an essential oil.liquid crystalline precursors, which are in situ gelling nanostructured surfactant systems, can undergo phase transition in aqueous solution and become more structured aggregates, controlling release of larvicides and acting as biotechnology alternatives for dengue control. such systems can contain bioactive substances as citrus sinensis essential oil (cseo) which exhibits biological activity against aedes aegypti (ae. aegypti) larvae.201526125515
susceptibility status of aedes aegypti (l.) (diptera: culicidae) to temephos from three districts of tamil nadu, india.dengue is the most rapidly expanding arboviral disease in india. aedes aegypti is the primary vector of dengue fever. chemical insecticides have long been used in the vector control programmes along with other control measures. however, continuous use of insecticides targeting ae. aegypti may lead to development of insecticide resistance. though resistance in ae. aegypti has been reported, the mutation in ace-1 gene associated with temephos resistance is not reported in natural populations. the ...201526119549
boric acid ovicidal trap for the management of aedes species.the use of low concentrations of boric acid as a potential and effective control agent for the eggs and immature stages of aedes aegypti l. and aedes albopictus skuse (diptera: culicidae) is found to be safe and effective as compared to synthetic chemical insecticides. the study aims to determine the ovicidal concentration of boric acid, its effective concentration for oviposition attraction and the larval mortality concentration for ae. aegypti and ae. albopictus.201526119547
physiological and biochemical effects of botanical extract from piper nigrum linn (piperaceae) against the dengue vector aedes aegypti liston (diptera: culicidae).the leaves of piper nigrum l. (piperaceae) were evaluated for chemical constituents and mosquito larvicidal activity against the larvae of aedes aegypti. gc and gc-ms analyses revealed that the crude extracts contain 16 compounds. thymol (20.77%) and ç-elemene (10.42%) were identified as the major constituents followed by cyclohexene, 4-ethenyl-4-methyl-3-(1-methylethenyl)-1-(1 methylethyl)-, (3r-trans) (7.58%), 4,6-octadienoic acid, 2-acetyl-2-methyl-, ethyl ester (6.98), 2(3h)-furanone, 3,4-bi ...201526277727
green synthesis of silver nanoparticles from cassia roxburghii-a most potent power for mosquito control.mosquitoes transmit serious human diseases, causing millions of deaths every year. the use of synthetic insecticides to control vector mosquitoes has caused physiological resistance and adverse environmental effects in addition to high operational cost. insecticides of synthesized natural products for vector control have been a priority in this area. in the present study, silver nanoparticles (agnps) synthesized using cassia roxburghii plant leaf extract against anopheles stephensi, aedes aegypt ...201526276645
candidate biomarkers for mosquito age-grading identified by label-free quantitative analysis of protein expression in aedes albopictus females.we applied a "shotgun" approach based on nanoliquid chromatography-high resolution mass spectrometry associated to label free quantification (lfq) to identify proteins varying with age, independently from the physiological state, in aedes albopictus, a mosquito species which in the last decades invaded temperate regions in north america and europe, creating concerns for associated high nuisance and risk of arbovirus transmission. the combined "shotgun" and lfq approach was shown to be highly sui ...201526271156
diversity and abundance of mosquitoes (diptera:culicidae) in an urban park: larval habitats and temporal variation.urban parks are areas designated for human recreation but also serve as shelter and refuge for populations of several species of native fauna, both migratory and introduced. in brazil, the effect of annual climate variations on aedes aegypti and dengue epidemics in large cities like são paulo is well known, but little is known about how such variations can affect the diversity of mosquito vectors in urban parks and the risk of disease transmission by these vectors. this study investigates the in ...201526259817
effect of ultraviolet-a radiation on the production of leptolegnia chapmanii (saprolegniales: saprolegniaceae) zoospores on dead aedes aegypti (diptera: culicidae) larvae and their larvicidal activity.impact of uv-radiation in entomopathogens in aquatic environments remains little investigated. the present study reports on the effect of uv-a on the larvicidal activity of leptolegnia chapmanii zoospores in aedes aegypti; on the production of zoospores in larvae killed by the pathogen and then exposed to uv-a; and on the activity of these zoospores against healthy larvae. whereas the virulence of free zoospores in a. aegypti larvae was affected by a uv-a exposure time longer than 10min, product ...201526259676
mosquitocidal and oviposition repellent activities of the extracts of seaweed bryopsis pennata on aedes aegypti and aedes albopictus.the ever-increasing threat from infectious diseases and the development of insecticide resistance in mosquito populations drive the global search for new natural insecticides. the aims of this study were to evaluate the mosquitocidal activity of the extracts of seaweed bryopsis pennata against dengue vectors aedes aegypti and aedes albopictus, and determine the seaweed's toxic effect on brine shrimp nauplii (as a non-target organism). in addition, the chemical compositions of the active larvicid ...201526247928
effect of chloroxylon swietenia dc bark extracts against culex quinquefasciatus, aedes aegypti, and anopheles stephensi larvae.mosquitoes are the vector of more diseases and cause major health problems like malaria, dengue, chikungunya, and lymphatic filariasis. this article deals with the mosquito larvicidal activity of chloroxylon swietenia dc bark extracts against late third instar larvae of culex quinquefasciatus, aedes aegypti, and anopheles stephensi. methanolic crude extract of ch. swietenia bark was obtained by soxhlet apparatus and aqueous crude extract by cold percolation method. the range of concentrations of ...201526246308
forced egg retention and oviposition behavior of malaria, dengue and filariasis vectors to a topical repellent diethyl-phenylacetamide.egg retention and oviposition behavior of four species of mosquito vectors viz., anopheles stephensi, aedes aegypti, aedes albopictus, and culex quinquefasciatus to a topical insect repellent diethyl-phenylacetamide (depa) at 0.1-1000 mg/l was investigated under laboratory conditions. based on oviposition activity indices, depa demonstrated concentration dependent oviposition deterrent effect to a. stephensi (-0.18 to -0.97), a. aegypti (-0.18 to -0.91) and a. albopictus (-0.50 to -0.98) females ...201526245028
plant-borne ovicides in the fight against mosquito vectors of medical and veterinary importance: a systematic review.mosquitoes (diptera: culicidae) are a huge threat for millions of people worldwide, since they act as vectors for devastating parasites and pathogens. culicidae control is of crucial importance. mosquito eggs, larvae, and pupae are usually targeted using organophosphates, insect growth regulators, and microbial agents. indoor residual spraying and insecticide-treated bed nets are also employed. however, these chemicals have negative effects on human health and the environment, and induce resista ...201526239801
[neonatal dengue. presentation of clinical cases].dengue is an infectious disease caused by a flavivirus, with four serotypes, transmitted by the mosquito aedes aegypti. in mexico it is a public health problem, especially in the region of central and southeast of the country. the disease can be asymptomatic or present serious forms and even death. it is confirmed by detection of the ns1 antigen; igm antibodies, polymerase chain reaction and virus isolation. the vertical transmission to de newborn has been little studied. 7 cases in neonates fro ...201526233977
host-feeding pattern of aedes aegypti and aedes albopictus (diptera: culicidae) in heterogeneous landscapes of south andaman, andaman and nicobar islands, india.mosquito foraging behavior is a determinant of host-vector contact and has an impact on the risk of arboviral epidemics. therefore, blood-feeding patterns is a useful tool for assessing the role in pathogen transmission by vector mosquitoes. competent vectors of dengue and chikungunya viz. aedes aegypti and aedes albopictus are widely prevalent in the andaman and nicobar archipelago. considering the vector potential, medical importance of both these mosquito species and lack of information on ho ...201526220560
tree holes as larval habitats for aedes aegypti in urban, suburban and forest habitats in a dengue affected area.aedes aegypti (l.) (diptera: culicidae), the main vector of dengue and urban yellow fever in the world, is highly adapted to the human environment. artificial containers are the most common larval habitat for the species, but it may develop in tree holes and other phytotelmata. this study assessed whether tree holes in san ramón de la nueva orán, a city located in subtropical montane moist forest where dengue outbreaks occur, are relevant as larval habitat for ae. aegypti and if the species may ...201526193903
characterization and biotoxicity of hypnea musciformis-synthesized silver nanoparticles as potential eco-friendly control tool against aedes aegypti and plutella xylostella.two of the most important challenges facing humanity in the 21st century comprise food production and disease control. eco-friendly control tools against mosquito vectors and agricultural pests are urgently needed. insecticidal products of marine origin have a huge potential to control these pests. in this research, we reported a single-step method to synthesize silver nanoparticles (agnp) using the aqueous leaf extract of the seaweed hypnea musciformis, a cheap, nontoxic and eco-friendly materi ...201526184431
evaluation of three commercial backpack sprayers with aqualuer® 20-20 against caged adult aedes aegypti.three commercially available backpack sprayers were evaluated with aqualuer® 20-20 (20.6% permethrin, active ingredient; 20.6% piperonyl butoxide, technical) against caged adult aedes aegypti in semifield trials in northeastern florida. two battery-powered sprayers, birchmeier and hudson, were compared with the standard hand-pump solo 425 sprayer, which is currently used in pest management operations. physical characteristics, droplet analysis, and overall ease of use were documented. multiple d ...201526181698
a trypsin inhibitor from clitoria fairchildiana cotyledons is active against digestive enzymes of aedes aegypti larvae.aedes aegypti, the principal mosquito vector of yellow fever, dengue fever and chikungunya fever virus-transmitted diseases, is an insect closely associated with humans and their housing habitats. as there is no commercially available vaccine, prevention is the most suggested form of avoiding disease spreading and a number of studies are being developed in order to give support to vector control operations. the present study reports on the identification of a trypsin inhibitor isolated from coty ...201526156641
evaluation of silver nanoparticles toxicity of arachis hypogaea peel extracts and its larvicidal activity against malaria and dengue vectors.silver nanoparticles (agnps) were successfully synthesised from aqueous silver nitrate using the extracts of arachis hypogaea peels. the synthesised snps were characterized by fourier transform-infrared spectroscopy analysis, x-ray diffraction, transmission electron microscopy analysis and high-resonance scanning electron microscopy, and energy dispersive x-ray spectroscopy. agnps were well defined and measured 20 to 50 nm in size. the nanoparticles were crystallized with a face-centered cubic s ...201526154036
[microgeographic and temporal genetic changes of aedes aegypti from medellín, colombia].aedes aegypti populations may experience changes in abundance and genetic diversity in addition to changes in their evolutionary capability to respond to vector control. the knowledge on the changes in genetic variation on a spatio-temporal scale improves the epidemiological understanding of dengue and supports the appropriate and timely design of vector control strategies.201526148034
[insecticide susceptibility evaluation in aedes aegypti populations of caldas, colombia, in 2007 and 2011].continuous use of insecticides for the control of dengue transmission may lead to decreased susceptibility levels in mosquito vector populations. timely monitoring is necessary to ensure detection of any potential resistance problems.201526148033
a simple and affordable membrane-feeding method for aedes aegpyti and anopheles minimus (diptera: culicidae).this study developed an artificial feeding (af) method to replace direct host feeding (dhf) for the maintenance of aedes aegypti and anopheles minimus mosquito colonies. the procedure can be adopted by all laboratories due to its simple and affordable materials and design. the apparatus consists of heparinized cow blood contained in a 5cm diameter glass petri dish with 5cm(2) parafilm m (bemis(®)) stretched thinly over the top, with a pre-heated bag of vegetable oil placed underneath to keep the ...201526440474
control of aedes aegypti and ae. albopictus, the vectors of dengue and chikungunya, by using pheromone c21 with an insect growth regulator: results of multicentric trials from 2007-12 in india.aedes mosquito control has gained much importance nowadays in view of rise in number of reported cases of dengue and chikungunya in india and other countries. in the present study, c21 attracticide (containing a pheromone and an insect growth regulator—igr, developed by defence research and development establishment (drde), gwalior, india was tested for its feasibility for surveillance and control of aedes mosquito in a multicentric mode from october 2007 to june 2012 in urban (delhi, and bengal ...201526418653
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