Publications

the vibrio cholerae toxr/tcpp/toxt virulence cascade: distinct roles for two membrane-localized transcriptional activators on a single promoter.	toxr is required in vibrio cholerae for transcriptional activation of the toxt gene, the protein product of which activates numerous genes involved in virulence. although toxr cannot activate the toxt promoter in escherichia coli, the products of the tcpph operon are shown here to activate the toxt promoter, and co-expression with toxrs enhances activation. an identical pattern was seen in a deltatcppdeltatoxr strain of v. cholerae when tcpph or toxrs was expressed from plasmids. although overex ...	2000	11029691
[molecular epidemiology of large bacterial endemics in sub-saharan africa].	over the past decades, the differentiation of bacterial strains for epidemiological purposes had been based on conventional phenotypic characters. more recently, methods studying the directly coded molecules or semantides (nucleic acids or proteins) have allowed, concomitantly with the technical progresses of electrophoresis, the description of stable, discriminant, reproducible markers, which were applicable to large series of isolates. initially applied to study nosocomial infections in indust ...	2000	11030062
in vivo covalent cross-linking of cellular actin by the vibrio cholerae rtx toxin.	enteric pathogens often export toxins that elicit diarrhea as a part of the etiology of disease, including toxins that affect cytoskeletal structure. recently, we discovered that the intestinal pathogen vibrio cholerae elicits rounding of epithelial cells that is dependent upon a gene we designated rtxa. here we investigate the association of rtxa with the cell-rounding effect. we find that v. cholerae exports a large toxin, rtx (repeats-in-toxin) toxin, to culture supernatant fluids and that th ...	2000	11032799
construction of a vibrio cholerae vaccine candidate using transposon delivery and flp recombinase-mediated excision.	recent efforts to develop a vaccine against the diarrheal disease cholera have focused on the use of live attenuated strains of the causative organism, vibrio cholerae. the ogawa lipopolysaccharide phenotype is expressed by many epidemic strains, and motility defects reduce the risk of reactive diarrhea in vaccine recipients. we therefore converted a motile inaba(+) vaccine candidate, peru-2, to a nonmotile ogawa(+) phenotype using a mariner-based transposon carrying rfbt, the gene required for ...	2000	11035750
construction and characterization of a nonproliferative el tor cholera vaccine candidate derived from strain 638.	in recent clinical assays, our cholera vaccine candidate strain, vibrio cholerae 638 el tor ogawa, was well tolerated and immunogenic in cuban volunteers. in this work we describe the construction of 638t, a thymidine auxotrophic version of improved environmental biosafety. in so doing, the thya gene from v. cholerae was cloned, sequenced, mutated in vitro, and used to replace the wild-type allele. except for its dependence on thymidine for growth in minimal medium, 638t is essentially indisting ...	2000	11035753
association of protease activity in vibrio cholerae vaccine strains with decreases in transcellular epithelial resistance of polarized t84 intestinal epithelial cells.	culture supernatants prepared from reactogenic strains of vibrio cholerae cause a decrease in the transcellular epithelial resistance of t84 intestinal cells. this decrease correlates with the presence of hemagglutinin/protease but not with the presence of other potential accessory toxins or proteases. these data suggest a possible role for hemagglutinin/protease in reactogenicity, although other factors may also contribute.	2000	11035765
a novel action of the proton pump inhibitor rabeprazole and its thioether derivative against the motility of helicobacter pylori.	the motility of helicobacter pylori was maximum at 37 degrees c and at ph 6. a newly developed proton pump inhibitor, rabeprazole (rpz), and its thioether derivative (rpz-th) markedly inhibited the motility of h. pylori. the concentrations of the drug necessary to inhibit 50% of the motility were 0.25, 16, 16, and >64 microgram/ml for rpz-th, rpz, lansoprazole, and omeprazole, respectively. no such inhibitory effects were observed with h(2) blockers or anti-h. pylori agents. the motilities of ca ...	2000	11036024
bactericidal activity of lemon juice and lemon derivatives against vibrio cholerae.	food products can be possible vectors of the agent responsible for cholera epidemics, because some of these products allow vibrio cholerae o1 to develop to concentrations above the dangerous level. this study deals with the behaviour of essential oils, natural and concentrated lemon juice and fresh and dehydrated lemon peel against v. cholerae o1 biotype eltor serotype inaba tox+. our aim was to evaluate whether these products, used at different dilutions, exhibit bactericidal or bacteriostatic ...	2000	11041258
active intestinal chloride secretion in human carriers of cystic fibrosis mutations: an evaluation of the hypothesis that heterozygotes have subnormal active intestinal chloride secretion.	to explain the very high frequency of cystic fibrosis (cf) mutations in most populations of european descent, it has been proposed that cf heterozygotes have a survival advantage when infected with vibrio cholerae or escherichia coli, the toxins of which induce diarrhea by stimulation of active intestinal chloride secretion. two assumptions underlie this hypothesis: (1) chloride conductance by the cf transmembrane conductance regulator (cftr) is the rate-limiting step for active intestinal chlor ...	2000	11055897
[experimental resistance of vibrio cholerae el tor to nalidixic acid and fluoroquinolones].	it was shown that sensitivity of vibrio cholerae eltor p-5879 to tetracycline, levomycetin, furazolidone, trimethoprim/sulfamethoxazole, aminoglycosides, beta-lactams, rifampicin, quinolones in vitro correlated with drugs efficacy in the treatment of experimental cholera of albino mice. mutants of v. cholerae eltor p-5879 nalr resistant to nalidixic acid (mic 160-200 mg/l) formed with frequency 10(-9)-110(-8) had no cross resistance to fluoroquinolones. but the efficacy of ofloxacin, lomefloxaci ...	2000	11057367
detection of virulence associated genes, haemolysin and protease amongst vibrio cholerae isolated in malaysia.	eighty-four strains of vibrio cholerae o1, o139 and non-o1/non-o139 from clinical and environmental sources were investigated for the presence of the toxin co-regulated pilus gene, tcpa, the virulence cassette genes ctxa, zot, ace and cep and also for their ability to elaborate haemolysin and protease. the ctxa and zot genes were detected using dna-dna hybridization while the ace, cep and tcpa genes were detected using pcr. production of haemolysin and protease was detected using mammalian eryth ...	2000	11057956
rapid method for species-specific identification of vibrio cholerae using primers targeted to the gene of outer membrane protein ompw.	the distribution of genes for an outer membrane protein (ompw) and a regulatory protein (toxr) in vibrio cholerae and other organisms was studied using respective primers and probes. pcr amplification results showed that all (100%) of the 254 v. cholerae strains tested were positive for ompw and 229 ( approximately 98%) of 233 were positive for toxr. none of the 40 strains belonging to other vibrio species produced amplicons with either ompw- or toxr-specific primers, while 80 bacterial strains ...	2000	11060082
emergence of vibrio cholerae o1 biotype el tor serotype inaba from the prevailing o1 ogawa serotype strains in india.	the toxigenic inaba serotype of vibrio cholerae o1 biotype el tor reappeared in india in 1998 and 1999, almost 10 years after its last dominance in calcutta in 1989. extensive molecular characterization by ribotyping, restriction fragment length polymorphism, and pulsed-field gel electrophoresis indicated that recent inaba strains are remarkably different from the earlier inaba strains but are very similar to the prevailing v. cholerae o1 ogawa el tor biotype strains. the antibiograms of the ina ...	2000	11060101
tetracycline resistant cholera in kelantan.	sensitivity testing on vibrio cholerae isolates during an epidemic in 1998 in kelantan identified strains resistant to tetracycline. this prompted a change in the usual management of cholera in kelantan. the antibiotic of choice was changed from tetracycline to erythromycin.	2000	11072502
inorganic polyphosphate in vibrio cholerae: genetic, biochemical, and physiologic features.	vibrio cholerae o1, biotype el tor, accumulates inorganic polyphosphate (poly p) principally as large clusters of granules. poly p kinase (ppk), the enzyme that synthesizes poly p from atp, is encoded by the ppk gene, which has been cloned from v. cholerae, overexpressed, and knocked out by insertion-deletion mutagenesis. the predicted amino acid sequence of ppk is 701 residues (81.6 kda), with 64% identity to that of escherichia coli, which it resembles biochemically. as in e. coli, ppk is part ...	2000	11073913
global spread of microorganisms by ships.		2000	11081499
human zonulin, a potential modulator of intestinal tight junctions.	intercellular tight junctions are dynamic structures involved in vectorial transport of water and electrolytes across the intestinal epithelium. zonula occludens toxin derived from vibrio cholerae interacts with a specific intestinal epithelial surface receptor, with subsequent activation of a complex intracellular cascade of events that regulate tight junction permeability. we postulated that this toxin may mimic the effect of a functionally and immunologically related endogenous modulator of i ...	2000	11082037
vibrio cholerae requires rpos for efficient intestinal colonization.	vibrio cholerae is a facultative intestinal pathogen that lives in aquatic environments, often in association with planktonic species. in the suckling mouse, oral inoculation with v. cholerae leads to intestinal colonization and symptoms of diarrheal disease. results reported here indicate a role for the alternative sigma factor, rpos, in intestinal colonization in this model of cholera. we constructed within rpos multiple independent mutations which consistently resulted in a fivefold decrease ...	2000	11083783
sequence analysis of tnphoa insertion sites in vibrio cholerae mutants defective in rugose polysaccharide production.	vibrio cholerae can switch from a smooth to a wrinkled or rugose colony phenotype characterized by the secretion of a polysaccharide that enables the bacteria to survive harsh environmental conditions. in order to understand the genetic basis of rugosity, we isolated tnphoa-induced stable, smooth mutants of two o1 el tor rugose strains and mapped the insertion sites in several of the mutants using a modified y-adapter pcr technique. one of the tnphoa insertions was mapped to the first gene of th ...	2000	11083805
phylogeny of vibrio cholerae based on reca sequence.	we sequenced a 705-bp fragment of the reca gene from 113 vibrio cholerae strains and closely related species. one hundred eighty-seven nucleotides were phylogenetically informative, 55 were phylogenetically uninformative, and 463 were invariant. not unexpectedly, vibrio parahaemolyticus and vibrio vulnificus strains formed out-groups; we also identified isolates which resembled v. cholerae biochemically but which did not cluster with v. cholerae. in many instances, v. cholerae serogroup designat ...	2000	11083852
ctx prophages in classical biotype vibrio cholerae: functional phage genes but dysfunctional phage genomes.	ctxphi is a filamentous, lysogenic bacteriophage whose genome encodes cholera toxin, the primary virulence factor produced by vibrio cholerae. ctx prophages in o1 el tor and o139 strains of v. cholerae are found within arrays of genetically related elements integrated at a single locus within the v. cholerae large chromosome. the prophages of o1 el tor and o139 strains generally yield infectious ctxphi. in contrast, o1 classical strains of v. cholerae do not produce ctxphi, although they produce ...	2000	11092860
monoclonal antibody ds6 detects a tumor-associated sialoglycotope expressed on human serous ovarian carcinomas.	a newly developed murine monoclonal antibody, ds6, immunohistochemically reacts with an antigen, ca6, that is expressed by human serous ovarian carcinomas but not by normal ovarian surface epithelium or mesothelium. ca6 has a limited distribution in normal adult tissues and is most characteristically detected in fallopian tube epithelium, inner urothelium and type 2 pneumocytes. pre-treatment of tissue sections with either periodic acid or neuraminidase from vibrio cholerae abolishes immunoreact ...	2000	11093807
cluster-analysis & patterns of dissemination of multidrug resistance among clinical strains of vibrio cholerae in calcutta, india.	antimicrobial resistance among vibrio cholerae has been monitored for several years in calcutta. to investigate the changing trends in multidrug resistance (mdr) among different serogroups of v. cholerae and to perform software assisted cluster analysis the current study was undertaken.	2000	11094852
environmental cues and gene expression in porphyromonas gingivalis and actinobacillus actinomycetemcomitans.	microorganisms typically adapt to environmental cues by turning on and off the expression of virulence genes which, in turn, allows for optimal growth and survival within different environmental niches. this adaptation strategy includes sensing and responding to changes in nutrients, ph, temperature, oxygen tension, redox potential, microbial flora, and osmolarity. for a bacterium to adhere to, penetrate, replicate in, and colonize host cells, it is critical that virulence genes are expressed du ...	2000	11355267
[a new look at the mechanism of cholera endemicity caused by vibrio cholerae biotype eltor].	cholera caused by vibrio cholerae biotype eltor (evc) is an endemic disease, subsiding in winter and reappearing in spring and summer. investigating the state of evc during the intermittent time is of crucial importance in controlling this disease.	2000	11775883
viable but nonculturable bacteria: a survival strategy.	when bacteria are introduced into a new environment, environmental changes with which they are confronted may include temperature, nutrient concentration, salinity, osmotic pressure, and ph. bacterial cells dynamically adapt to these shifts in their environment, employing a variety of genetic mechanisms. bacteria, with the ability to utilize constitutive and inducible enzyme synthesis, can accommodate to growth-limiting nutrients and adjust or reroute metabolic pathways to avoid metabolic and/or ...	2000	11810550
serotypes and phage types of vibrio cholerae in mysore.	a total of 565 samples of acute diarrhoeal stools from children received over a period of twelve months from november 1996 to october 1997, yielded 289 (51.15%) isolates of v. cholerae. out of these, 277 (95.85%) were v. cholerae 01 serotype ogawa, 7 (2.42%) were v. cholerae 0139 and 5 (1.73%) were v. cholerae non-o1 non-0139. phage typing of the isolates was carried out by niced calcutta. out of 78 isolates, 69 (88.46%) belong to phage type t27. of the remaining, 3 (3.85%), 3(3.85%), 2(2.56%) a ...	2000	11668944
[simultaneous detection and differentiation of o1 classical e1 tor, o139 and nono1 nono139 strains of vibrio cholerae by using multiplex pcr].	to explore the rapid and sensitive procedures for detection and differentiation of o1 classical(cvc), el tor(evc), o139 and nono1 nono139 strains of vibrio cholerae.	2000	11372395
[cholera 1].		2000	11385767
[cholera 2].		2000	11385820
an outbreak of eltor cholera in aizwal town of mizoram, india.	during the months of may, june and through early part of july 1994, an unusual occurrence of severe dehydrating watery diarrhoea cases and deaths were reported from aizwal town, the capital of mizoram, a north-eastern state of india. vibrio cholerae 01 biotype eltor, the causative agent responsible for this outbreak, was isolated from 50.0% of hospitalised cases. the disease affected older children and adults more (52.9%) than younger children below five years of age. vibrio cholerae 01 strains ...	2000	11407007
mechanical carrier of bacterial enteric pathogens by chrysomya megacephala (diptera: calliphoridae) in chiang mai, thailand.	chrysomya megacephala was studied regarding its mechanically bacterial carrier in urban areas of chiang mai, northern thailand. fifty-six adult flies were randomly collected using sweep insect net during april-may, 1999 from 3 fresh food markets and examined for bacteriological isolation. among them, 49 flies (87.5%) were bacterial carriers. the total 22 bacterial species and 8 groups were isolated. three species previously reported as the bacterial enteric pathogens causing diarrheal disease we ...	2000	11414447
epidemiology of cholera outbreak in kampala, uganda.	to provide epidemiological description of the cholera outbreak which occurred in kampala between december 1997 and march 1998.	2000	12862150
antimicrobial susceptibility pattern of vibrio cholerae o1 strains during two cholera outbreaks in dar es salaam, tanzania.	to determine and compare the antimicrobial susceptibility patterns of vibrio cholerae 01 strains, which were isolated in two cholera epidemics in 1997 and 1999 in dar es salaam.	2000	12862151
[dr. d. nicasio landa, official epidemic doctor during the cholera epidemic of 1854-1855].	in this unpublished work from 1861, which we have transcribed, dr. nicasio landa (1830-1891) is revealed to have been at the forefront in carrying out epidemiological studies in spain. he traced the medical topography, or geographical pathology, of cholera by provinces, by means of calculating the incidence rate ("millesimal proportion of those infected") and lethality rates ("millesimal proportion of the dead") using the official data on the sick and deceased in the cholera epidemic of 1854-185 ...	2000	12886322
[microbiological quality of street-vendor ice cream in dakar].	during a multicenter study initiated by the international network of pasteur institutes and associated institutes, microbiological quality of street-vended ice creams in dakar was evaluated. 313 samples of ice creams from 170 street-vendors were collected and tested for common foodborne pathogens and indicator organisms. results showed that microbiological quality of 45% of tested samples was unsatisfactory because of large populations of aerobic mesophilic organisms (36.7%), thermotolerant coli ...	2000	14666784
notes on the spectrum of opiate use.		2000	97557
characterization of fractional mutations in drosophila: role of rna, protein & repair synthesis.		2000	94307
[a delicate intervention: tonsillectomy in adults].		2000	92060
can the family physician avoid conflict of interest in the gatekeeper role? an affirmative view.		2000	2723596
acyclovir for treatment of mucocutaneous herpes infection in a child with leukaemia.		2000	91931
[pancreatic exocrine function in diseases of the digestive organs].		2000	316614
[biochemical basis of parenteral feeding. composition of the organism and its response to fasting].		2000	97736
[sialic acid content and igg binding of the glycocalyx of preserved erythrocytes].	fractions of light and heavy erythrocytes were separated by centrifugation from blood samples banked in acd-ag medium at 4 degrees c for periods up to 6 weeks. both light and heavy erythrocytes have lost about 4,9% of their content of sialic acids during banking for 6 weeks. this reduction is in accord with a 6%-decrease of their agglutination by means of alcian blue. it is, however, a variance with the inhibition of agglutination by anti-igg sera. the present findings provide evidence for the r ...	2000	89989
historical perspectives of the united states air force, army, navy, public health service, and veterans administration nursing services.		2000	97587
phenomenological aspects of phencyclidine abuse among ethnic groups in hawaii.		2000	101869
antimicrobial potency of the leaf - stalk extract of curcuma longa (linn).	the methanolic extract of the leaf-stalk of curcuma longa linn, was tested for its minimum inhibitor concentration (mic) against gram positive-staphylococcus aureus, bacillus pumilus, bacillus subtilis, klebsiella pnemoniae, bacillus cereus, streptococcus pneumoniae, lactobacillus arabinosus and gram negative e.coli, shigella dysenteriae, shigella sonnei, shigella boydii, salmonella typhimurium, proteus mirabilis, and vibrio cholerae strains, further, the ones of inhibition produced by the crude ...	2000	22557005
endocrine testicular function in mink during the first year of life.	the endocrine testicular function in mink (mustela vison) was investigated during the first year of life encompassing puberty, the first mating season and the phase of regression thereafter. the mink, relatively easily accessible as a semi-domesticated animal, was chosen as an example of a mammalian seasonal breeder. in plasma samples from 7 to 17 animals collected on 10 occasions between july and april testosterone and delta4-androstenedione were determined by simultaneous radioimmunoassay. a s ...	2000	1173496
the suprahyoid muscles of the tufted capuchin (cebus apella).		2000	116332
[questions and answers on heart diseases. 8. heart auscultation].		2000	112302
conflagration in an ambulance oxygen system.		2000	1127787
[beta-mimetics and tachyphylaxis].	this study covers current knowledge concerning the risks of tachyphylaxis induced by prolonged use of beta-mimetics in the asthmatic. the organization of the adrenergic membrane system is reviewed, as well as the various mechanisms which may lead to desensitization. experimental evidence of the latter is described. the most extensive studies have involved circulating cells and, more precisely, lymphocytes and polynuclear neutrophils. clinical trials are then dealt with. studies concerned with br ...	2000	2869566
[bedside nursing. assistance for a patient with puerperal neurosis associated with stress of child care].		2000	112295
[détermination of salivary ph by contact ph metry. study of ph on the tongue and of the orifices of wharton's and stenon's ducts].	the authors describe an electrochemical technique for the measurement of salivary ph by contact. they present a critical study fo 167 cases. the values obtained are characteristic in terms of age and the site of determination (dorsal surface of the tongue, orifice of wharton's or stenon's ducts).	2000	26965
the efficacy of oral zinc therapy as an alternative to penicillamine for wilson's disease.		2000	3336426
topical ocular administration of cromolyn sodium for treatment in seasonal ragweed conjunctivitis.		2000	112137
[determination of relative humidity of gas mixtures to be inspired during endotracheal anesthesia and ventilation].		2000	4688745
a case of a right coronary artery to left ventricle fistula observed over twenty years.	since the natural history of a coronary artery to left ventricle fistula is not well known, a case of such a fistula in 36-year-old female patient, followed for 20 years is presented. two-dimensional echocardiography performed as a follow-up examination revealed enlargement of the right coronary artery and the right valsalva sinus. two-dimensional color flow mapping showed the jet flow to the apex from the base of the left ventricle only during the diastolic phase. an aortogram confirmed that en ...	2000	2810924
[significance of immuno-serological studies in chronic hepatopathies].		2000	97683
replication of poly da and poly ra by a drosophila dna polymerase.	the activity of a 7.3s-8.3s drosophila dna polymerase was characterized in detail using poly da.p(dt)[unk] and poly ra.p(dt)[unk]. with poly da.p(dt)[unk], mg(2+) ion was the preferred divalent cation, and enzyme activity was inhibited by k(+) ion and by spermidine. with poly ra.p(dt)[unk], mn(2+) ion was the preferred divalent cation and enzyme activity was stimulated by k(+) ion and by spermidine. the dependence of enzyme activity on the concentration of primer-template and on the ratio of pri ...	2000	97638
[diagnosis of mediterranean eruptive fever by indirect immunofluorescence].		2000	384363
[factor analysis of bone mineral dynamics in patients with renal osteodystrophy].	remarkably high accumulation of tc-99m-mdp is seen in the skull on bone scintigraphy of patients with renal osteodystrophy (rod), especially with secondary hyperparathyroidism. for the quantitative evaluation, the factor analysis (fa) was used for the early phase of bone scan. tc-99m-mdp (14.8 mbq/body weight kg) was injected as a bolus through the medial antecubital vein. dynamic acquisition of 40 x 30 sec frames were performed in a 64 x 64 matrix. for pre-processing, nine points smoothing was ...	2000	2810901
external baccalaureate degrees for physician assistants.		2000	16251
[pulmonary embolism precipitated after swimming: report of a case of spontaneous resolution].		2000	2640858
three types of paranoid processes.	in classical descriptive psychiatry the term 'paranoid' is often used ambiguously -- referring to a variety of clinical processes which should be more clearly differentiated. specifically, in this paper, we have differentiated three distinct sets of clinical phenomena all usually lumped together as 'paranoid': 1. paranoid from a sense of guilt, 2. paranoid from a sense of low self-esteem, and 3. paranoid from a sense of persecution. these three processes are distinct descriptively, dynamically a ...	2000	14813
[six channels telemetry system for recording masticatory forces of complete denture wearers].	a six channels telemetry system for recording masticatory force of complete denture wearers has been developed. this system is consisted of three blocks, the transmission block, the receiver block and the data storage and correction block. the transmission block is embedded in a lower complete denture. system has six load sensors and a standard resistor to correct sensor data. data from six load sensors and standard resistor as well as standard zero are multiplexed using a division multiplex com ...	2000	2810879
a note on the birth of a baboon (papio anubis).		2000	97191
[serum beta 2-microglobulin and renal function (author's transl)].		2000	89679
lateral wall of the olfactory fossa in determining intracranial extension of sinus carcinomas.	the role of plain skull radiography and pluridirectional tomography in determining anterior cranial fossa extension of paranasal sinus tumors is reassessed. the lateral wall of the olfactory fossa, a thin bony plate forming part of the roof of the ethmoid sinuses, is routinely visualized on plain skull radiography and pluridirectional tomography in the coronal plane and serves as an indicator of intracranial tumor extension. in a series of 47 patients with paranasal sinus tumors, 100% true posit ...	2000	111511
[economic crisis in public health in the capitalist countries].		2000	97790
[therapy of cronh's disease in the acute stage, using ornidazole (tiberal). preliminary results].	9 patients with acute exacerbations of crohn's disease were treated over 2.5 months or longer with ornidazole (nitroimidazole). while 3 patients showed no response at all to 5 cycles of ornidazole treatment, 6 patients improved markedly within 2-3 months. in all but 1 patient, the improvement lasted during long-term therapy (average 9 months). therapy was discontinued in 5 of these patients after 2.5-14 months; 2 patients had severe recurrences within 2-10 weeks, while 3 patients remained well d ...	2000	97778
hypertensive complications in patients with aldosterone-producing adenomas.		2000	87647
a novel biological function of macrophages associated with antigen discrimination properties.	the ability of fresh or cultured subpopulation of adherent peritoneal exudate cells to perform discrete biological functions commonly associated with macrophages, namely antibody dependent cytotoxicity (adcc) and phagocytosis, has been compared with the ability of the same cells to present tnp coupled to carbohydrate or protein carriers for a subsequent antibody response, or semi-allogeneic cells for t cell cytotoxic responses, in tissue culture. data are presented to show that adcc reactivity r ...	2000	94755
fluorimetric assay for prenatal detection of hurler and scheie homozygotes or heterozygotes.		2000	87574
early detection of cancer of oesophagus along caspian littoral. report of a pilot project.	a simple mass-screening procedure for the early detection by brush cytology of oesophageal cancer in 280 adults in high-incidence villages of the caspian littoral is described. the presence of atypical cells in 7%, and the discovery of frank carcinoma in 3 symptom-free people encourages continuation of the project.	2000	87555
[renal insufficiency in congenital renal changes with special reference to a personal series].		2000	112375
[rights and responsibilities of the patient-consumer. the french-language federation of consumers attacks health care costs].		2000	102092
nursing assistants - an untapped resource in providing quality care to the elderly ill.		2000	101575
[whole-body radiometry using methionine-75se for determining intrahepatic cholestasis].		2000	101729
anomolous staining patterns in immunohistologic studies of malignant lymphoma.	a number of immunoperoxidase studies of malignant lymphomas have reported polytypic light chain staining of neoplastic cells, thus bringing into question the concept that the monoclonality of b cell lymphomas is reflected in their synthesis of monotypic light chain. in this study of a large number of ig positive lymphomas, staining for a wide variety of antigens has identified clear differences between monotypic ig synthesizing cells and cells staining polytypically which appear to be taking up ...	2000	90079
[treatment of pain with psychotropic drugs (author's transl)].	30 patients suffering from therapy-resistant chronic pain were divided into three groups according to drive and mood: i. psychomotor inhibition combined with depressive mood, ii. agitation and depressive mood, iii. agitation combined normal mood. the psychopharmacological treatment of pain showed sufficient results, when the drugs were directed to the target symptoms "agitation", "lack of drive", and "depression". the differentiated administration of activating or sedative antidepressants and ne ...	2000	27829
[treatment of acute chloroquine poisoning].	acute chloroquine poisoning is life threatening with risk of death from apnoea and cardiac arrhythmia within a few hours of ingestion. mechanical ventilation, infusion of pressor agents and large doses of diazepam seem to provide effective treatment. this treatment was introduced by riou et alii (n engl j med 1988; 318; 1-6), and we used it successfully in a case of severe chloroquine poisoning. intensive treatment was given during the first two days of intoxication, when the whole blood chloroq ...	2000	2327037
perinatal perspective: introduction to this issue of the qrb.		2000	101945
clinical trial of topical disodium chromoglycate in vernal keratoconjunctivitis.	the clinical picture of spring catarrh was studied in 96 cases. the duration of the disease was found to be 3.5 +/- 2.6 years. it exists all the year round with a higher frequency between april and august. male children were affected about three times more frequently than females. the effect of dscg was studied in 20 cases. it had a significant therapeutic effect. we advise a combined dscg and corticosteroid treatment in the period of exacerbation.	2000	101923
validity of some clinical signs as early indicators of protein calorie malnutrition in preschool children.		2000	98441
[acute renal insufficiency in hemorrhagic fever with nephrotic syndrome].		2000	27875
pus, deoxyribonucleic acid, and sputum viscosity.	on 100 sputum specimens selected from patients suffering from chronic bronchitis, bronchiectasis, asthma, and cystic fibrosis total deoxyribonucleic acid (dna) content has been related to macroscopic type, to total dry weight yield, and to the apparent viscosity of the secretion at 1350 s-1: since dna may be present, either as fibres or within cells, in one-third of the specimens the contribution of each form to the apparent viscosity was assessed. the effect on sputum viscosity of the addition ...	2000	26989
the localization of trnaasp2 genes from drosophila melanogaster by "in situ" hybridization.	transfer rnaasp2delta was isolated from drosophila melanogaster by affinity chromatography on concanavalin a-sepharose. the trna was iodinated "in vitro" with na [125i] and hybridized "in situ" to salivary gland chromosomes from drosophila. subsequent autoradiography allowed the localization of the genes for trnaasp2 to the left arm of the second chromosome in the regions 29 d and e.	2000	101767
evidence for the presence of an oncornaviral reverse transcriptase in an orbital tumor associated to acute myelomonocytic leukemia in children: biochemical and immunological characterization of the enzyme.		2000	94581
[arrhythmias in direct fetal electrocardiography].		2000	92107
fluorescence staining of living cells with fluorescamine.		2000	87001
genital herpes zoster: a case resembling primary herpes progenitalis with distal urethral involvement.		2000	101907
oral methionine loading as a cause of acute serum folate deficiency: its relevance to parenteral nutrition.	oral methionine supplements (8.0 g daily for 4 days) were given to five normal volunteers who continued to eat their usual diet. this treatment resulted in a significant fall in serum folate concentration. three days after the end of treatment concentrations had not completely returned to control values. the fall in concentration was prevented by giving oral folic acid supplements. it is suggested that folic acid supplements should be given to patients who are receiving intravenous infusions of ...	2000	97644
meeting exceptional nutritional needs. 1. total parenteral nutrition.	the provision of adequate nutrition to hospitalized patients with exceptional caloric requirements has been a problem until the recent advent of intravenous hyperalimentation. with total parenteral nutrition (tpn), the nutritional needs of any patient can be met by infusion. tpn solution is hypertonic, and administration requires central venous cannulation. the subclavian vein is usually chosen as route of access to the superior vena cava. strict aseptic technique must be used in inserting the c ...	2000	97643
selected genodermatoses.		2000	97616
a generalized response to protracted stress?		2000	97593
transference phenomena in the treatment of addictive illness: love and hate in methadone maintence.		2000	97533
[activities of a home helper. mobile bathing service].		2000	97458
[emergency care and nursing. 17. tetanus, gas gangrene, foreign bodies, drowning, and electrocution].		2000	97454
morphological abnormalities in cultured erythroid colonies (bfu-e) from the blood of two patients with hempas.	the results of cytological and ultrastructural analysis of erythroid burst colonies derived from the peripheral blood of two patients with hempas have been compared to those obtained in normal controls. using the plasma clot technique, in studies on 10 subjects we confirmed that most of the colonies consisted or erythroblasts with a synchronous and normal maturation involving a wave of nuclear extrusion at day 13. in contrast, the majority of well-haemoglobinized colonies from hempas consisted o ...	2000	89861