Publications

pharmacological and genetic evidence for gap junctions as potential new insecticide targets in the yellow fever mosquito, aedes aegypti.	the yellow fever mosquito aedes aegypti is an important vector of viral diseases that impact global health. insecticides are typically used to manage mosquito populations, but the evolution of insecticide resistance is limiting their effectiveness. thus, identifying new molecular and physiological targets in mosquitoes is needed to facilitate insecticide discovery and development. here we test the hypothesis that gap junctions are valid molecular and physiological targets for new insecticides. g ...	2015	26325403
metazoan trna introns generate stable circular rnas in vivo.	we report the discovery of a class of abundant circular noncoding rnas that are produced during metazoan trna splicing. these transcripts, termed trna intronic circular (tric)rnas, are conserved features of animal transcriptomes. biogenesis of tricrnas requires anciently conserved trna sequence motifs and processing enzymes, and their expression is regulated in an age-dependent and tissue-specific manner. furthermore, we exploited this biogenesis pathway to develop an in vivo expression system f ...	2015	26194134
increasing dengue incidence in singapore over the past 40 years: population growth, climate and mobility.	in singapore, the frequency and magnitude of dengue epidemics have increased significantly over the past 40 years. it is important to understand the main drivers for the rapid increase in dengue incidence. we studied the relative contributions of putative drivers for the rise of dengue in singapore: population growth, climate parameters and international air passenger arrivals from dengue endemic countries, for the time period of 1974 until 2011. we used multivariable poisson regression models w ...	2015	26322517
involvement of antizyme characterized from the small abalone haliotis diversicolor in gonadal development.	the small abalone haliotis diversicolor is an economically important mollusk that is widely cultivated in southern china. gonad precocity may affect the aquaculture of small abalone. polyamines, which are small cationic molecules essential for cellular proliferation, may affect gonadal development. ornithine decarboxylase (odc) and antizyme (az) are essential elements of a feedback circuit that regulates cellular polyamines. this paper presents the molecular cloning and characterization of az fr ...	2015	26313647
effective disposal of nitrogen waste in blood-fed aedes aegypti mosquitoes requires alanine aminotransferase.	to better understand the mechanisms responsible for the success of female mosquitoes in their disposal of excess nitrogen, we investigated the role of alanine aminotransferase (alat) in blood-fed aedes aegypti. transcript and protein levels from the 2 alat genes were analyzed in sucrose- and blood-fed a. aegypti tissues. alat1 and alat2 exhibit distinct expression patterns in tissues during the first gonotrophic cycle. injection of female mosquitoes with either double-stranded rna (dsrna)-alat1 ...	2015	26310269
de novo transcriptome assembly and analysis to identify potential gene targets for rnai-mediated control of the tomato leafminer (tuta absoluta).	providing double-stranded rna (dsrna) to insects has been proven to silence target genes, and this approach has emerged as a potential method to control agricultural pests by engineering plants to express insect dsrnas. a critical step of this technology is the screening of effective target genes essential for insect development and/or survival. the tomato leafminer (tuta absoluta meyrick) is a major solanum lycopersicum (tomato) pest that causes significant yield losses and has recently invaded ...	2015	26306628
a novel olfactory pathway is essential for fast and efficient blood-feeding in mosquitoes.	in mosquitoes, precise and efficient finding of a host animal is crucial for survival. one of the poorly understood aspects of mosquito blood-feeding behavior is how these insects target an optimal site in order to penetrate the skin and blood vessels without alerting the host animal. here we provide new findings that a piercing structure of the mouthpart of the mosquitoes, the stylet, is an essential apparatus for the stage in blood feeding. indeed, the stylet possesses a number of sensory hair ...	2015	26306800
estimation of the basic reproductive ratio for dengue fever at the take-off period of dengue infection.	estimating the basic reproductive ratio ℛ 0 of dengue fever has continued to be an ever-increasing challenge among epidemiologists. in this paper we propose two different constructions to estimate ℛ 0 which is derived from a dynamical system of host-vector dengue transmission model. the construction is based on the original assumption that in the early states of an epidemic the infected human compartment increases exponentially at the same rate as the infected mosquito compartment (previous work ...	2015	26413140
a catalog of proteins expressed in the ag secreted fluid during the mature phase of the chinese mitten crabs (eriocheir sinensis).	the accessory gland (ag) is an important component of the male reproductive system of arthropods, its secretions enhance fertility, some ag proteins bind to the spermatozoa and affect its function and properties. here we report the first comprehensive catalog of the ag secreted fluid during the mature phase of the chinese mitten crab (eriocheir sinensis). ag proteins were separated by one-dimensional gel electrophoresis and analyzed by reverse phase high-performance liquid chromatography coupled ...	2015	26305468
risk factors associated with asthma, atopic dermatitis and rhinoconjunctivitis in a rural senegalese cohort.	the world allergy organization estimates that 40 % of the world's population is affected by allergic diseases. the international study of asthma and allergies in childhood has completed phase iii and it has now become clear that these diseases have increased in developing countries, especially africa, where prevalence rates were formerly low. despite an increase in studies in africa, few sub-saharan west african countries are represented; the focus has remained on urban populations and little at ...	2015	26306096
β carbonic anhydrase is required for female fertility in drosophila melanogaster.	carbonic anhydrases (cas, ec 4.2.1.1) are ubiquitous enzymes that catalyze the reversible hydration reaction of carbon dioxide. cas are present as six structurally divergent enzyme families: α, β, γ, δ, ζ and η. β-cas have a wide distribution across different species including invertebrates. previously, we showed that drosophila melanogaster β-ca is a highly active mitochondrial enzyme. in this study, we investigated the function of drosophila β-ca by silencing the expression of the β-ca gene us ...	2015	26300950
genome analysis and polar tube firing dynamics of mosquito-infecting microsporidia.	microsporidia are highly divergent fungi that are obligate intracellular pathogens of a wide range of host organisms. here we review recent findings from the genome sequences of mosquito-infecting microsporidian species edhazardia aedis and vavraia culicis, which show large differences in genome size, although similar numbers of predicted genes. we also show a video of e. aedis polar tube firing, which is the dramatic mechanism used by microsporidia to deliver the germ cell (sporoplasm) into the ...	2015	26300319
oxidative stress and maxi calcium-activated potassium (bk) channels.	all cells contain ion channels in their outer (plasma) and inner (organelle) membranes. ion channels, similar to other proteins, are targets of oxidative impact, which modulates ion fluxes across membranes. subsequently, these ion currents affect electrical excitability, such as action potential discharge (in neurons, muscle, and receptor cells), alteration of the membrane resting potential, synaptic transmission, hormone secretion, muscle contraction or coordination of the cell cycle. in this c ...	2015	26287261
energy homeostasis control in drosophila adipokinetic hormone mutants.	maintenance of biological functions under negative energy balance depends on mobilization of storage lipids and carbohydrates in animals. in mammals, glucagon and glucocorticoid signaling mobilizes energy reserves, whereas adipokinetic hormones (akhs) play a homologous role in insects. numerous studies based on akh injections and correlative studies in a broad range of insect species established the view that akh acts as master regulator of energy mobilization during development, reproduction, a ...	2015	26275422
chitosan: an update on potential biomedical and pharmaceutical applications.	chitosan is a natural polycationic linear polysaccharide derived from chitin. the low solubility of chitosan in neutral and alkaline solution limits its application. nevertheless, chemical modification into composites or hydrogels brings to it new functional properties for different applications. chitosans are recognized as versatile biomaterials because of their non-toxicity, low allergenicity, biocompatibility and biodegradability. this review presents the recent research, trends and prospects ...	2015	26287217
spatial and temporal clustering of chikungunya virus transmission in dominica.	using geo-referenced case data, we present spatial and spatio-temporal cluster analyses of the early spread of the 2013-2015 chikungunya virus (chikv) in dominica, an island in the caribbean. spatial coordinates of the locations of the first 417 reported cases observed between december 15th, 2013 and march 11th, 2014, were captured using the global positioning system (gps). we observed a preponderance of female cases, which has been reported for chikv outbreaks in other regions. we also noted st ...	2015	26274813
vector competence of aedes aegypti mosquitoes for filarial nematodes is affected by age and nutrient limitation.	mosquitoes are one of the most important vectors of human disease. the ability of mosquitoes to transmit disease is dependent on the age structure of the population, as mosquitoes must survive long enough for the parasites to complete their development and infect another human. age could have additional effects due to mortality rates and vector competence changing as mosquitoes senesce, but these are comparatively poorly understood. we have investigated these factors using the mosquito aedes aeg ...	2015	25446985
chikungunya: here today, where tomorrow?		2015	25576501
a mosquito survey of the twin-island caribbean nation of saint kitts and nevis, 2010.	adult mosquito surveys of saint kitts and nevis (skn) were performed in the dry season (march 16-23, 2010) in saint kitts, and the rainy season (october 18-25, 2010) in skn. biogents (bg) sentinel traps were set with co₂and bg lure in urban, rural, mangrove, and dry forest habitats. mosquitoes were identified to species, and reverse transcription-polymerase chain reaction was performed on potential vector species for dengue virus (denv), chikungunya virus (chikv), and west nile virus (wnv). the ...	2015	26675458
chikungunya.	chikungunya is an acute viral disease characterised by fever and painful arthralgia. the arthritic symptoms associated with chikungunya can be debilitating and may persist for months or even years in some patients. severe neurological complications such as encephalitis have also been reported during recent large outbreaks. the disease is caused by chikungunya virus (chikv), a mosquito-borne alphavirus from the togaviridae family, which has recently emerged to become one of the most important exo ...	2015	26601450
historical inability to control aedes aegypti as a main contributor of fast dispersal of chikungunya outbreaks in latin america.	the arrival of chikungunya fever (chikf) in latin american countries has been expected to trigger epidemics and challenge health systems. historically considered as dengue-endemic countries, abundant aedes aegypti populations make this region highly vulnerable to chikungunya virus (chikv) circulation. this review describes the current dengue and chikf epidemiological situations, as well as the role of uncontrolled ae. aegypti and aedes albopictus vectors in spreading the emerging chikv. comments ...	2015	26518229
arbovirus surveillance and first report of chikungunya virus in wild populations of aedes aegypti from guerrero, mexico.	we carried out dengue (denv) and chikungunya virus (chikv) surveillance in wild populations of aedes aegypti from guerrero, mexico, from 2012 to 2014 following a standard national protocol of the mexican dengue control program. a total of 284 pools (15-30 specimens/pool) of female mosquitoes were tested with real-time reverse transcriptase-polymerase chain reaction to detect denv and chikv. we report for the 1st time the detection of chikv from field-collected mosquitoes at acapulco and juchitán ...	2015	26375910
assessing carbon dioxide and synthetic lure-baited traps for dengue and chikungunya vector surveillance (3).	the aedes mosquito vectors of dengue virus (denv) and chikungunya virus (chikv) are attracted to specific host cues that are not generated by traditional light traps. for this reason multiple companies have designed traps to specifically target those species. recently the standard trap for denv and chikv vectors, the bg-sentinel (bgs) trap, has been remodeled to be more durable and better suited for use in harsh field conditions, common during military operations, and relabeled the bg-sentinel 2 ...	2015	26375905
[lessons learned in the control of aedes aegypti to address dengue and the emergency of chikungunya in iquitos, peru].	dengue has affected iquitos since 1990 causing outbreaks of major impact on public health and for this reason great efforts have been made for its temporal control. currently, with the expansion of the chikungunya virus in the americas and the threat of the emergence of the virus in iquitos, we reflect on lessons learned by way of the activities undertaken in the area of vector control; epidemiological surveillance, diagnosis and clinical management during periods of outbreaks of dengue, in a wa ...	2015	26102121
susceptibility of aedes albopictus and aedes aegypti to three imported chikungunya virus strains, including the e1/226v variant in taiwan.	an e1/226v variant chikungunya virus (chikv) efficiently transmitted by aedes albopictus to humans poses a significant threat to public health for those areas with the presence of aedes albopictus, including taiwan.	2015	25715998
comparative efficacy of existing surveillance tools for aedes aegypti in western kenya.	all traditional surveillance techniques for aedes aegypti have been developed for the cosmopolitan domestic subspecies ae. aegypti aegypti, and not the sylvatic subspecies, ae. aegypti formosus. the predominant form in western kenya is ae. aegypti formosus that is rarely associated with human habitations but is linked to transmission of sylvatic dengue virus strains. we compared five surveillance methods for their effectiveness in sampling ae. aegypti formosus with the goal of determining a sust ...	2015	26611965
natural vertical transmission of dengue viruses in aedes aegypti in selected sites in cebu city, philippines.	we attempted to determine the vertical transmission of dengue virus (denv) in aedes aegypti in selected sites in cebu city, philippines. mosquito sub-adults were collected monthly from households and the field during the wet-dry-wet season from november, 2011 to july, 2012 and were laboratory-reared to adults. viral rna extracts in mosquitoes were assayed by hemi-nested rt-pcr. results showed that 62 (36.26%; n=679) out of 171 mosquito pools (n=2,871) were denv+. the minimum infection rate (mir) ...	2015	26611963
innate antiviral immunity against dengue virus.	dengue virus (denv), the most prevalent mosquito-borne viral diseases in humans worldwide, causes dengue fever, a mild form of the disease, as well as dengue hemorrhagic fever/dengue shock syndrome, a more severe form which can be life-threatening. the four serotypes of denv (denv1-4) are positive-sense, single stranded rna virus belonging to the flaviviridae family and are transmitted by aedes aegypti and aedes albopictus mosquitoes. together, they are estimated to cause almost 100 million symp ...	2015	26559229
[estimation of aedes aegypti (l.) (diptera: culicidae) productivity in households and public spaces in a dengue endemic city in colombia].	aedes aegypti is a vector for the dengue virus in colombia. its productivity can be estimated using pupal counts.	2015	26535548
essential oils from zanthoxylum fagara wild lime, ruta chalepensis l. and thymus vulgaris l.: composition and activity against aedes aegypti larvae.	the dengue virus is transmitted by aedes aegypti. several plants are used to control this mosquito. in the present study the chemical composition of the essential oils of ruta chalepensis, zanthoxylum fagara and thymus vulgaris were analyzed, and their activities against larvae of two a. aegypti populations were evaluated. the major compounds found in t. vulgaris were thymol and -cymene at 39.8% and 30.5%, respectively, with the major components being oxygenated monoterpenes and monoterpene hyd ...	2015	26525020
dengue fever and dengue hemorrhagic fever in adults.	dengue fever and dengue hemorrhagic fever are re-emerging diseases that are endemic in the tropics. the global prevalence of dengue cases has increased in south-east asia, africa, the western pacific, and the americas. the increasingly widespread distribution and the rising incidence of dengue virus infections are related to increased distribution of aedes aegypti, an increasingly urban population, and increasing air travel. several southeast asian countries show that the age of the reported den ...	2015	26506734
a role for vector control in dengue vaccine programs.	development and deployment of a successful dengue virus (denv) vaccine has confounded research and pharmaceutical entities owing to the complex nature of denv immunity and concerns over exacerbating the risk of denv hemorrhagic fever (dhf) as a consequence of vaccination. thus, consensus is growing that a combination of mitigation strategies will be needed for denv to be successfully controlled, likely involving some form of vector control to enhance a vaccine program. we present here a determin ...	2015	26478199
assessment of the relationship between entomologic indicators of aedes aegypti and the epidemic occurrence of dengue virus 3 in a susceptible population, são josé do rio preto, são paulo, brazil.	the aims of this study were to describe the occurrence of dengue in space and time and to assess the relationships between dengue incidence and entomologic indicators. we selected the dengue autochthonous cases that occurred between september 2005 and august 2007 in são josé do rio preto to calculate incidence rates by month, year and census tracts. the monthly incidence rates of the city were compared to the monthly breteau indices (bi) of the são josé do rio region. between december 2006 and f ...	2015	25484110
dengue virus 4 (denv-4) re-emerges after 30 years in brazil: cocirculation of denv-2, denv-3, and denv-4 in bahia.	dengue fever (df) is a mosquito-borne viral disease of great concern in tropical and subtropical regions of the world. one important cause of the increase in df is rapid development and urbanization has led to proliferation of the aedes aegypti mosquito, the vector responsible for transmission of the illness. surveillance of dengue virus (denv) infection in brazil shows the predominance of denv-1, denv-2, and denv-3 until 2010. this study reports the reappearance of denv-4 in brazil for the firs ...	2015	25420650
dengue fever in europe: could there be an epidemic in the future?	dengue virus (denv) is the arbovirus with the widest impact on human health. although its dispersal is partially conditioned by environmental constraints that limit the distribution of its main vector (aedes aegypti), denv has been spreading geographically in recent times, but mostly afflicting tropical and subtropical regions. with no prophylactic vaccine or specific therapeutics available, vector control remains the best alternative to restrain its circulation. moreover, the establishment of t ...	2015	25399556
protein-protein interactions between a. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cdna library.	dengue virus (denv) is principally transmitted by the aedes aegypti mosquito. to date, mosquito population control remains the key strategy for reducing the continuing spread of denv. the focus on the development of new vector control strategies through an understanding of the mosquito-virus relationship is essential, especially targeting the midgut, which is the first mosquito organ exposed to denv infection.	2015	26719940
first evidence of dengue virus infection in wild caught mosquitoes during an outbreak in assam, northeast india.	dengue is one of the major public health problems worldwide, transmitted mainly by aedes aegypti and ae. albopictus mosquitoes. rapid urbanisation and industrialisation have led to an increase in vector population in northeastern states of india. in 2013, guwahati, the capital city of assam, india experienced an outbreak of dengue. this study was undertaken with an objective to determine infection rates of dengue viruses (denv) in both the established vectors present in this region.	2015	26714508
erratum to: nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae).		2015	26412056
sterol carrier protein 2, a critical host factor for dengue virus infection, alters the cholesterol distribution in mosquito aag2 cells.	host factors that enable dengue virus (denv) to propagate in the mosquito host cells are unclear. it is known that cellular cholesterol plays an important role in the life cycle of denv in human host cells but unknown if the lipid requirements differ for mosquito versus mammalian. in mosquito aedes aegypti, sterol carrier protein 2 (scp-2) is critical for cellular cholesterol homeostasis. in this study, we identified scp-2 as a critical host factor for denv production in mosquito aag2 cells. tre ...	2015	26336241
comparison of the insecticidal characteristics of commercially available plant essential oils against aedes aegypti and anopheles gambiae (diptera: culicidae).	aedes aegypti and anopheles gambiae are two mosquito species that represent significant threats to global public health as vectors of dengue virus and malaria parasites, respectively. although mosquito populations have been effectively controlled through the use of synthetic insecticides, the emergence of widespread insecticide-resistance in wild mosquito populations is a strong motivation to explore new insecticidal chemistries. for these studies, ae. aegypti and an. gambiae were treated with c ...	2015	26336230
detection of dengue virus in mosquito extracts and human clinical samples using a field expedient molecular platform.	dengue fever occurs in localized outbreaks and can significantly erode troop strength and mission readiness. timely identification of dengue virus (denv) provides for rapid and appropriate patient management decisions, such as medical evacuation and supportive therapies, as well as help to promote force health protection through vector control and personal protective measures. the "ruggedized" advanced pathogen identification device is a field-friendly pcr (polymerase chain reaction) platform th ...	2015	26327544
nanoparticles in the fight against mosquito-borne diseases: bioactivity of bruguiera cylindrica-synthesized nanoparticles against dengue virus den-2 (in vitro) and its mosquito vector aedes aegypti (diptera: culicidae).	mosquitoes are blood-feeding insects serving as the most important vectors for spreading human pathogens and parasites. dengue is a viral disease mainly vectored through the bite of aedes mosquitoes. its transmission has recently increased in urban and semi-urban areas of tropical and subtropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depend on effective vector control measures. mangrov ...	2015	26290219
role of aedes aegypti and aedes albopictus during the 2011 dengue fever epidemics in hanoi, vietnam.	to record the human cases of dengue fever (df) and investigate the aedes mosquito species circulating during the hanoi 2011 df epidemics.	2015	26276285
different serotypes of dengue virus (denv) imported by polish travellers from dengue endemic areas to poland.	dengue viruses are the most widespread arboviruses (transmitted mainly by aedes aegypti and ae. albopictus mosquitoes), which have shown an unexpected geographic expansion. there are four dengue virus serotypes: denv-1, denv-2, denv-3, and denv-4. subsequent infections increase the risk of developing severe dengue fever.	2015	26119675
detection of dengue virus in individual aedes aegypti mosquitoes in delhi, india.	delhi, the capital city of india, has so far witnessed several outbreaks of dengue fever since 1967 (last one reported in 2013). improved virological and entomological surveillance are the only tools that can help in prevention of dengue as well as in the development of dengue control programmes. the aim of the study was to conduct a prospective field study to detect dengue virus in adult aedes aegypti mosquitoes collected from various localities represented by different socioeconomic groups in ...	2015	26119544
[vertical transmission of dengue virus in aedes aegypti, peru].		2015	26102125
[dengue in peru: a quarter century after its reemergence].	a health problem each time more frequent and dispersed in tropical and subtropical areas of the world, including peru where it entered in 1990, is dengue. it is produced by the dengue virus with four serotypes and transmitted by aedes aegypti, a vector that coexists with humans and whose presence is favored by deficient sanitary, social and economic conditions. manifestations of severe forms of the disease such as shock and bleeding, are related to the frequent co-circulation of the four serotyp ...	2015	26102118
green-synthesized silver nanoparticles as a novel control tool against dengue virus (den-2) and its primary vector aedes aegypti.	dengue is an arthropod-borne viral infection mainly vectored through the bite of aedes mosquitoes. recently, its transmission has strongly increased in urban and semi-urban areas of tropical and sub-tropical regions worldwide, becoming a major international public health concern. there is no specific treatment for dengue. its prevention and control solely depends on effective vector control measures. in this study, we proposed the green-synthesis of silver nanoparticles (agnp) as a novel and eff ...	2015	26063530
development and validation of an arthropod maceration protocol for zoonotic pathogen detection in mosquitoes and fleas.	arthropod-borne diseases remain a pressing international public health concern. while progress has been made in the rapid detection of arthropod-borne pathogens via quantitative real-time (qpcr), or even hand-held detection devices, a simple and robust maceration and nucleic acid extraction method is necessary to implement biosurveillance capabilities. in this study, a comparison of maceration techniques using five types of beads followed by nucleic acid extraction and detection were tested usin ...	2015	26047188
dengue virus detection in aedes aegypti larvae from southeastern brazil.	the transmission of dengue, the most important arthropod-borne viral disease in brazil, has been intensified over the past decades, along with the accompanying expansion and adaptation of its aedes vectors. in the present study, we mapped dengue vectors in ouro preto and ouro branco, minas gerais, by installing ovitraps in 32 public schools. the traps were examined monthly between september, 2011 through july, 2012 and november, 2012 to april, 2013. the larvae were reared until the fourth stadiu ...	2015	26047186
sero-prevalence of dengue infections in the kassala state in the eastern part of the sudan in 2011.	dengue fever is a vector-borne disease that is transmitted to humans by infected aedes aegypti mosquitoes. the eastern part of the sudan is one of the regions that is affected by dengue virus circulation. in this study, we estimated the prevalence of dengue infections in the kassala state in the eastern part of the sudan. the study objective was to estimate the sero-prevalence of dengue igg/igm antibodies in the kassala locality in 2011. this was a cross sectional community-based study that util ...	2015	25975993
natural transovarial transmission of dengue virus 4 in aedes aegypti from cuiabá, state of mato grosso, brazil.	dengue is the most prevalent arboviral disease in tropical areas. in mato grosso, outbreaks are reported every year, but studies on dengue in this state are scarce.	2015	25860459
susceptibility of indigenous and transplanted mosquito spp. to dengue virus in japan.	dengue fever, an acute, mosquito-borne, febrile illness caused by flavivirus spp., is a problem in africa, south and southeast asia, latin america, and the caribbean. a dengue outbreak occurred after nearly 70 years of absence or no detection, and then 158 autochthonous cases occurred in japan from august to october 15, 2014. the most competent mosquito vectors for dengue virus transmission were aedes aegypti and a. albopictus. since a. albopictus is widely distributed across japan and a. aegypt ...	2015	25766611
the consequences of co-infections for parasite transmission in the mosquito aedes aegypti.	co-infections may modify parasite transmission opportunities directly as a consequence of interactions in the within-host environment, but also indirectly through changes in host life history. furthermore, host and parasite traits are sensitive to the abiotic environment with variable consequences for parasite transmission in co-infections. we investigate how co-infection of the mosquito aedes aegypti with two microsporidian parasites (vavraia culicis and edhazardia aedis) at two levels of larva ...	2015	25311642
functional implications of the peptidoglycan recognition proteins in the immunity of the yellow fever mosquito, aedes aegypti.	peptidoglycan recognition proteins (pgrps) play essential roles in the immune systems of insects and higher animals against certain pathogens, including bacteria. in insects, most studies on the functions of pgrps have been performed in drosophila, with only limited studies in mosquitoes, which are important disease vectors. in the present study, we analysed the pgrp sequences of the yellow fever mosquito, aedes aegypti, acquired from two genome databases, and identified a total of seven pgrp ge ...	2015	25588548
molecular characterization of an apolipophorin-iii gene from the chinese oak silkworm, antheraea pernyi (lepidoptera: saturniidae).	apolipophorin-iii (apolp-iii) acts in lipid transport, lipoprotein metabolism, and innate immunity in insects. in this study, an apolp-iii gene of antheraea pernyi pupae (ap-apolp-iii) was isolated and characterized. the full-length cdna of ap-apolp-iii is 687 bp, including a 5'-untranslated region (utr) of 40 bp, 3'-utr of 86 bp and an open reading frame of 561 bp encoding a polypeptide of 186 amino acids that contains an apolipophorin-iii precursor domain (pf07464). the deduced ap-apolp-iii pr ...	2015	25348706
interaction of lysinibacillus sphaericus binary toxin with mosquito larval gut cells: binding and internalization.	the binary toxin produced by lysinibacillus sphaericus is composed of bina and binb subunits. together, but not separately, the two subunits are highly toxic to culex quinquefasciatus larvae, but show no toxicity to aedes aegypti. the molecular mechanism underlying intoxication has not been clearly elucidated. the present study compares the binding and the internalization of binary toxin into the midgut epithelial cells of susceptible c. quinquefasciatus mosquito larvae with those of bin-refract ...	2015	26408968
effects of artesunate treatment on plasmodium gallinaceum transmission in the vectors aedes aegypti and culex quinquefasciatus.	in the absence of vaccines, chemotherapy is an effective and economical way for controlling malaria. development of anti-malarial drugs that target pathogenic blood stage parasites and gametocytes is preferable for the treatment as it can alleviate the host's morbidity and mortality and block transmission of the plasmodium parasite. recently, our laboratory has developed an in vivo transmission blocking assay that involves administration of 7 consecutive daily doses of a test compound into domes ...	2015	25466617
behavioral response of aedes aegypti (diptera: culicidae) larvae to synthetic and natural attractants and repellents.	aedes aegypti (l.) (diptera: culicidae) is the key vector of three important arboviral diseases: dengue, yellow fever, and chikungunya. immature stages of this species inhabit human-made containers placed in residential landscapes. in this study, we evaluated a few compounds in a sensitive behavioral assay with ae. aegypti larvae. the orientation of larvae to different compounds was surveyed using a performance index (pi). the pi represents the response to each odorant, where a value of +1 is in ...	2015	26352935
efficient transposition of the youngest miniature inverted repeat transposable element family of yellow fever mosquito in yeast.	miniature inverted repeat transposable elements (mites) are often the most numerous dna transposons in plant and animal genomes. the dramatic amplification of mite families during evolution is puzzling, because the transposase sources for the vast majority of mite families are unknown. the yellow fever mosquito genome contains > 220-mb mite sequences; however, transposition activity has not been demonstrated for any of the mite families. the gnome elements are the youngest mite family in this ge ...	2015	25754725
mosquito larvicidal and pupaecidal potential of prodigiosin from serratia marcescens and understanding its mechanism of action.	mosquitoes spread lethal diseases like malaria and dengue fever to humans. considering mosquito vector control as one of the best alternatives to reduce new infections, here we have analyzed the effect of purified pigment prodigiosin extracted from serratia marcescens (nmcc 75) against larval and pupal stages of aedes aegypti and anopheles stephensi mosquitoes. mosquito larvicidal activities of purified prodigiosin revealed lc50 values of 14 ± 1.2, 15.6 ± 1.48, 18 ± 1.3, 21 ± 0.87 µg/ml against ...	2015	26267052
di-rhamnolipid is a mosquito pupicidal metabolite from pseudomonas fluorescens (vcrc b426).	pseudomonas fluorescens migula (vcrc b426) produces a secondary metabolite, which was found to be active against pupae of vector mosquitoes namely culex quinquefasciatus, anopheles stephensi and aedes aegypti. the mosquito pupicidal metabolite from p. fluoescens was mass produced and separated by ethyl acetate extraction and purified further by silica gel column chromatography, fplc, hplc and tlc. the purified metabolite was characterized by nmr, ft-ir, lc-ms and maldi-tof. the ft-ir, (1)h and ( ...	2015	25912083
inter-epidemic abundance and distribution of potential mosquito vectors for rift valley fever virus in ngorongoro district, tanzania.	background rift valley fever (rvf) is a mosquito-borne viral zoonosis that primarily affects ruminants but also has the capacity to infect humans. objective to determine the abundance and distribution of mosquito vectors in relation to their potential role in the virus transmission and maintenance in disease epidemic areas of ngorongoro district in northern tanzania. methods a cross-sectional entomological investigation was carried out before the suspected rvf outbreak in october 2012. mosquitoe ...	2015	28156815
[detection of flavivirus in mosquitoes (diptera: culicidae) from easter island-chile].	flaviviruses are arthropod-borne viruses, mainly by mosquitoes of the genera aedes and culex (culicidae) that are detected in tropical and subtropical areas. main flaviviruses of public health importance are: dengue, west nile virus, yellow fever, among others. in continental chile, flaviviruses has not been detected. however, there are indigenous cases of dengue detected in easter island since 2002, as the presence of its vector aedes aegypti. the aim of this study was: to determine diversity o ...	2015	25860055
ovicidal and adulticidal potential of leaf and seed extract of albizia lebbeck (l.) benth. (family: fabaceae) against culex quinquefasciatus, aedes aegypti, and anopheles stephensi (diptera: culicidae).	several diseases are associated to the mosquito-human interaction. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world. in the present study, hexane, benzene, chloroform, ethyl acetate, and methanol extracts of leaf and seed of albizia lebbeck were assayed for their toxicity agai ...	2015	25681143
laboratory evaluation of indian medicinal plants as repellents against malaria, dengue, and filariasis vector mosquitoes.	mosquito-borne diseases have an economic impact, including loss in commercial and labor outputs, particularly in countries with tropical and subtropical climates; however, no part of the world is free from vector-borne diseases. mosquitoes are the carriers of severe and well-known illnesses such as malaria, arboviral encephalitis, dengue fever, chikungunya fever, west nile virus, and yellow fever. these diseases produce significant morbidity and mortality in humans and livestock around the world ...	2015	25399815
virulency of novel nanolarvicide from trichoderma atroviride against aedes aegypti (linn.): a clsm analysis.	aedes aegypti is the vector for transmitting dengue, chikungunya, and yellow fever. these diseases' transmission has increased predominantly in urban and semi-urban areas as a major public health concern. in present investigation, trichoderma atroviride culture filtrates were used for the synthesis of silver nanoparticle. moreover, t. atroviride is a free-living and rapidly growing fungi common in soil and root ecosystem. this fungi is an exceptionally good model for biocontrol and more signific ...	2015	25907629
efficacy of tolypocladium cylindrosporum against aedes aegypti eggs, larvae and adults.	the aim of this study was to test the activity of seven tolypocladium cylindrosporum isolates against different developmental stages of aedes aegypti.	2015	26332164
chemical composition and biological activity of essential oils of dracocephalum heterophyllum and hyssopus officinalis from western himalaya.	the essential oils of two representatives of the lamiaceae, dracocephalum heterophyllum benth. and hyssopus officinalis l., are described for their antifungal, antibacterial, larvicidal and inect biting deterrent activities. additionally, the chemical compositions of the essential oils, analyzed by simultaneous gc-ms and gc-fid, and odor descriptions are given. the main components of h. officinalis oil were pinocarvone, cis-pinocamphone, and β-pinene. citronellol was found as the main compound o ...	2015	25920235
effects of proteinase inhibitor from adenanthera pavonina seeds on short- and long term larval development of aedes aegypti.	currently, one of the major global public health concerns is related to the transmission of dengue/yellow fever virus by the vector aedes aegypti. the most abundant digestive enzymes in ae. aegypti midgut larvae are trypsin and chymotrypsin. since protease inhibitors have the capacity to bind to and inhibit the action of insect digestive proteinases, we investigated the short- and long-term effects of adenanthera pavonina seed proteinase inhibitor (apti) on ae. aegypti larvae, as well as a possi ...	2015	25796215
[management of the yellow fever epidemic in 2010 in séguéla (côte d'ivoire): value of multidisciplinary investigation].	in august 2010, five positive cases of yellow fever were reported in the region of séguéla, in the northwest of côte d'ivoire, affected by an armed conflict since 2002. in order to evaluate the extent of yellow fever virus circulation and the risk for local people, a multidisciplinary investigation was carried out by the côte-d'ivoire ministry of health and public hygiene.	2015	25629680
evaluating liquid and granular bacillus thuringiensis var. israelensis broadcast applications for controlling vectors of dengue and chikungunya viruses in artificial containers and tree holes.	the principal vectors of chikungunya and dengue viruses typically oviposit in water-filled artificial and natural containers, including tree holes. despite the risk these and similar tree hole-inhabiting mosquitoes present to global public health, surprisingly few studies have been conducted to determine an efficient method of applying larvicides specifically to tree holes. the stihl sr 450, a backpack sprayer commonly utilized during military and civilian vector control operations, may be suita ...	2015	26335473
susceptibility profile of aedes aegypti from santiago island, cabo verde, to insecticides.	in 2009, cabo verde diagnosed the first dengue cases, with 21,137 cases reported and aedes aegypti was identified as the vector. since the outbreak, chemical insecticides and source reduction were used to control the mosquito population. this study aimed to assess the susceptibility of a. aegypti populations from santiago, cabo verde to insecticides and identify the mechanisms of resistance. samples of a. aegypti eggs were obtained at two different time periods (2012 and 2014), using ovitraps in ...	2015	26307496
the genetic architecture of a complex trait: resistance to multiple toxins produced by bacillus thuringiensis israelensis in the dengue and yellow fever vector, the mosquito aedes aegypti.	the bacterial insecticide bacillus thuringiensis subsp. israelensis (bti) is an increasingly popular alternative to chemical insecticides for controlling mosquito populations. because bti toxicity relies on the action of four main toxins, resistance to bti is very likely a complex phenotype involving several genes simultaneously. dissecting the underlying genetic basis thus requires associating a quantitative measure of resistance to genetic variation at many loci in a segregating population. he ...	2015	26238211
cry4ba and cyt1aa proteins from bacillus thuringiensis israelensis: interactions and toxicity mechanism against aedes aegypti.	individual crystal proteins from bacillus thuringiensis israelensis exhibit variable levels of insecticidal activities against mosquito larvae. in all cases, they are much less active compared to the whole crystal proteins due to described complex synergistic interactions among them. in the present study we investigated the effects of cyt1a98 (a cyt1aa type protein) on cry4blb (a cry4ba type toxin) insecticidal activity toward the dengue vector aedes aegypti. the bioassay analyses demonstrated t ...	2015	26238170
evidence of two mechanisms involved in bacillus thuringiensis israelensis decreased toxicity against mosquito larvae: genome dynamic and toxins stability.	biopesticides based on bacillus thuringiensis israelensis are the most used and most successful around the world. this bacterium is characterized by a dynamic genome able to win or lose genetic materials which leads to a decrease in its effectiveness. the detection of such phenomena is of great importance to monitor the stability of b. thuringiensis strains in industrial production processes of biopesticides. new local b. thuringiensis israelensis isolates were investigated. they present variabl ...	2015	26070692
effects of the p20 protein from bacillus thuringiensis israelensis on insecticidal crystal protein cry4ba.	the accessory protein p20 from bacillus thuringiensis israelensis has been defined as an important molecular chaperone for forming crystal cyt1aa, and enhancing cry11aa and cry4aa expression. to investigate its putative role in cry4ba delta-endotoxin production and toxicity, a p20 gene was cloned and introduced into b. thuringiensis recombinant strain expressing cry4ba type gene (cry4blb). the delta-endotoxin synthesis was enhanced by 262%. the generated inclusions were assayed against third ins ...	2015	25931398
two specific membrane-bound aminopeptidase n isoforms from aedes aegypti larvae serve as functional receptors for the bacillus thuringiensis cry4ba toxin implicating counterpart specificity.	the interaction between bacillus thuringiensis cry toxins and their receptors on midgut cells of susceptible insect larvae is the critical determinant in toxin specificity. besides gpi-linked alkaline phosphatase in aedes aegypti mosquito-larval midguts, membrane-bound aminopeptidase n (aaeapn) is widely thought to serve as a cry4ba receptor. here, two full-length aaeapn isoforms, aaeapn2778 and aaeapn2783, predicted to be gpi-linked were cloned and successfully expressed in spodoptera frugiperd ...	2015	25871797
maintenance of residual activity of bt toxin by using natural and synthetic dyes: a novel approach for sustainable mosquito vector control.	mosquito control protein from bacillus thuringiensis gets inactivated with exposure to sunlight. to address this issue, the potential of synthetic and natural dye was investigated as sunlight protectants. bt sv2 in absence of dyes when exposed to sunlight showed reduced effectiveness against the fourth instars of mosquito larvae. whereas acriflavin, congo red and violacein were able to maintain 86.4%, 91.6% and 82.2% mosquito larvicidal efficacy of bt sv2 against ivth instars larvae of anopheles ...	2015	25699646
functional assembly of 260-kda oligomers required for mosquito-larvicidal activity of the bacillus thuringiensis cry4ba toxin.	oligomerization has been shown to contribute to the toxicity of bacillus thuringiensis cry toxins. mutations have been made in the cry4ba toxin and resulted in toxic to non-toxic mutants toward aedes aegypti larvae. in this study, cry4ba wild type and mutants were analyzed for oligomer formation in vitro, biochemical properties and their relationships with larvicidal activity. in vitro, the cry4ba forms two-main types of the oligomers including (1) the 260-kda and larger oligomers, which assembl ...	2015	25687547
characterisation of novel bacillus thuringiensis isolates against aedes aegypti (diptera: culicidae) and ceratitis capitata (diptera: tephridae).	bacillus thuringiensis is successfully used in pest management strategies as an eco-friendly bioinsecticide. isolation and identification of new strains with a wide variety of target pests is an ever growing field. in this paper, new b. thuringiensis isolates were investigated to search for original strains active against diptera and able to produce novel toxins that could be used as an alternative for the commercial h14 strain. biochemical and molecular characterization revealed a remarkable di ...	2015	25433312
[vital study of the fatty body of larvae of aedes aegypti l. infected with densonucleosis virus].		2015	67557
extracellular synthesis of silver nanoparticles using bacillus megaterium against malarial and dengue vector (diptera: culicidae).	biosynthesis of silver nanoparticles has provoked nowadays and alternative to physical and chemical approaches. in the present study, silver nanoparticles (agnps) were synthesized extracellular method using bacillus megaterium. the agnps formations were confirmed initially through color change, and the aliquots were characterized through uv-visible spectrophotometer, followed by scanning electron microscopy (sem), energy-dispersive x-ray (edx) spectroscopy, and fourier transform infrared (ftir) ...	2015	26231835
[ecology of aedes aegypti and aedes albopictus in the americas and disease transmission].	the recent range expansion of the mosquito aedes albopictus has been associated in some areas by declines in abundance or local elimination of aedes aegypti, but the two species still coexist in large regions of the americas. we offer a summary of the possible mechanisms responsible for the abundance and displacement pattern observed and of their significance in terms of disease transmission. among these mechanisms we may mention the competition for limiting resources, the differences in the abi ...	2015	26535539
s argassum muticum-synthesized silver nanoparticles: an effective control tool against mosquito vectors and bacterial pathogens.	mosquito-borne diseases represent a deadly threat for millions of people worldwide. furthermore, pathogens and parasites polluting water also constitute a severe plague for populations of developing countries. in this research, silver nanoparticles (agnp) were synthesized using the aqueous extract of the seaweed sargassum muticum. the production of agnp was confirmed by surface plasmon resonance band illustrated in uv-vis spectrophotometry. agnp were characterized by ftir, sem, edx, and xrd anal ...	2015	26281786
insecticidal potency of aspergillus terreus against larvae and pupae of three mosquito species anopheles stephensi, culex quinquefasciatus, and aedes aegypti.	microbial control agents offer alternatives to chemical pest control, as they can be more selective than chemical insecticides. the present study evaluates the mosquito larvicidal and pupicidal potential of fungus mycelia using ethyl acetate and methanol solvent extracts produced by aspergillus terreus against anopheles stephensi, culex quinquefasciatus, and aedes aegypti. the a. terreus mycelia were extracted after 15 days from sabouraud dextrose broth medium. the ethyl acetate extracts showed ...	2015	26139412
genome mining offers a new starting point for parasitology research.	parasites including helminthes, protozoa, and medical arthropod vectors are a major cause of global infectious diseases, affecting one-sixth of the world's population, which are responsible for enormous levels of morbidity and mortality important and remain impediments to economic development especially in tropical countries. prevalent drug resistance, lack of highly effective and practical vaccines, as well as specific and sensitive diagnostic markers are proving to be challenging problems in p ...	2015	25563615
[effect of bacillus thuringiensis var. israelensis (bti) on detoxification enzyme activity of larvae of culex pipiens pallens and aedes aegypti].	to investigate the effect of bacillus thuringiensis var. israelensis (bti) on the activities of three detoxification enzymes of culex pipiens pallens and aedes aegypti larvae.	2015	26767261
bacillus thuringiensis subsp. israelensis producing endochitinase chia74δsp inclusions and its improved activity against aedes aegypti.	the objective of this study was to produce stable inclusions of chitinase chia74δsp in bacillus thuringiensis subsp. israelensis (bti) and to assay its insecticidal activity against aedes aegypti larvae.	2015	26434743
[identification of microrna-like viral small rnas from aaedv].	several virus families have been shown to encode micrornas (mirnas), which have roles in the infection and replication of viruses in host cells. these virus-encoded mirnas are identified in double-stranded dna virus (dsdna virus) and in several rna virus families, but not in single-stranded dna virus (ssdna virus). we used a bioinformatics approach based on vmir, mirnafold and maturepred software to predict virus-encoded mirna-like small rnas from the genome of a ssdna virus: aedes aegypti denso ...	2015	26738292
[predatory capacity of macrobrachium tenellum on aedes aegypti larvae in lab conditions].	in the last few years, a lot of importance has been given to natural predators against aedes aegypti. several organisms have been studied both in lab and in the field so as to find out their capacity to devour mosquito larvae. high densities of macrobrachium tenellum are found in natural conditions, it is not aggressive and may stand wide ranges of temperature, rates of salinity and oxygen concentrations.	2015	23424807
comparison among homemade repellents made with cloves, picaridin, andiroba, and soybean oil against aedes aegypti bites.		2015	22231260
comparison of field and laboratory-based tests for behavioral response of aedes aegypti (diptera: culicidae) to repellents.	the repellent and irritant effects of three essential oils-clove, hairy basil, and sweet basil-were compared using an excito-repellency test system against an insecticide-resistant strain of aedes aegypti (l.) females from pu teuy, kanchanaburi province. deet was used as the comparison standard compound. tests were conducted under field and controlled laboratory conditions. the most marked repellent effect (spatial noncontact assay) among the three test essential oils was exhibited by sweet basi ...	2015	26470388
[competition between aedes aegypti and aedes albopictus larvae in the laboratory].	this study had the aim of evaluating the effects of intra and interspecies larval competition between aedes aegypti and aedes albopictus, survival of larvae time taken to develop and wing length. the experiment was carried out with three densities and five proportions of the species. aedes aegypti survival was greater than aedes albopictus survival at intermediate density, while it was lower at high density. only the time taken for aedes albopictus to develop was affected. the differences found ...	2015	19009190
[prevalence of reactions secundary to mosquito bites aedes aegypti at en el regional center of allergy and clinical immunology, university hospital, de monterrey, nuevo leon].	although systemic reactions resulting from hymenoptera stings have been studied extensively, the prevalence of allergic reactions to mosquitoes is unknown.	2015	20857628
[epidemics and disease during the revolution period in mexico].	the health condition in mexico was bad around de beginning of the revolutionary period. the movement of troops led the development of epidemics like yellow fever, typhus, smallpox, and influenza that were enhance with natural disasters and hunger in whole country, from cost to cost and in the north big cities like monterrey, guadalajara and saltillo. doctor liceaga conducted a well planned campaign against yellow fever eradicating water stagnant deposits in order to combat the vector transmissio ...	2015	20929620
travel-related mosquito-transmitted disease questionnaire survey among health professionals in taiwan.	health-care professionals can help travelers by providing accurate pre-travel counseling for mosquito-transmitted diseases such as malaria, yellow fever, and dengue fever. governments and international organizations will benefit from knowledge survey among health professionals in this field to promote the development of travel health profession. this study investigates physicians' and nurses' knowledge regarding malaria, yellow fever, and dengue fever.	2015	21199140
[zika virus: another emerging arbovirus in venezuela?].	current health conditions of venezuela, together with the tropical proliferation of different types of aedes (aegypti and albopictus), suggest the arrival to this country of zika virus (zikv). the pan american health organization urges latin american countries to take measures to prevent the zikv entry and advises that these measures must be designed to detect the introduction of zikv in an area, track its spread and actively monitor the disease. in venezuela and other latin-american countries, ...	2015	26710538
screening for mosquito larvicidal activity of thai mushroom extracts with special reference to steccherinum sp against aedes aegypti (l.) (diptera: culicidae).	for over 50 years, biological control of mosquito larvae has depended mainly on plant extracts, fish, bacteria, protozoa, filamentous fungi, viruses or nematodes. in this study, we screened 143 mushroom samples from 44 confirmed species in thailand for their mosquito larvicidal activity. one g% (w/v) aqueous extracts of dried powdered mushroom samples were tested against 3rd stage aedes aegypti larvae. four mushroom species, namely, thaeogyroporus porentosus, xylaria nigripes, chlorophyllum sp a ...	2015	26867377
absence of aedes aegypti (l.) on an ecological island: competitive exclusion?	ovitrap surveillance was conducted in 2012 and 2006 in malay and aboriginal villages on carey island. in each village, standard ovitraps were placed indoors and outdoors at randomly selected houses/locations. all l3 larvae recovered were identified up to species level. results demonstrated that only larvae of aedes albopictus were found in all the positive ovitraps placed indoors and outdoors. in 2012, a high ovitrap index (oi) of 66.7% indoor and 84.0% outdoor in the malay village; and 62.5% in ...	2015	26863856