Publications

struvite calculi.	although calcium oxalate stones are the most common type of calculi found in the united states, struvite or infection stones are quite common and generally pose a difficult treatment dilemma. the presence of urinary infection with a urease-producing organism is necessary for these stones to form. proteus species account for the majority of infections that cause struvite stones in all age ranges. however, other organisms also produce urease and may be detected in conjunction with struvite calculi ...	1996	8890398
a simple method to detect bacteriolytic enzymes produced by enterobacteriaceae.	the production of bacteriolytic enzymes by enterobacteriaceae in various growth conditions was investigated. peptone-based media containing killed gram-negative cells facilitated detection of bacteriolytic enzyme production in the highest number of species. these belonged to the genera serratia, proteus, morganella and providencia. in contrast, escherichia coli, shigella, salmonella, klebsiella, enterobacter and citrobacter species did not produce bacteriolytic activities in any of the condition ...	1996	8893522
hyperammonemic encephalopathy in a four-year-old child with prune belly syndrome.		1996	8895934
ferryl intermediates of catalase captured by time-resolved weissenberg crystallography and uv-vis spectroscopy.	various enzymes use semi-stable ferryl intermediates and free radicals during their catalytic cycle, amongst them haem catalases. structures for two transient intermediates (compounds i and ii) of the nadph-dependent catalase from proteus mirabilis (pmc) have been determined by time-resolved x-ray crystallography and single crystal microspectrophotometry. the results show the formation and transformation of the ferryl group in the haem, and the unexpected binding of an anion during this reaction ...	1996	8901874
effect of urinary tract infection on disintegration of calcium-containing stones.	to evaluate the possible effect of urinary tract infection (uti) on the fragility of calcium-containing stones (calcium-oxalate, calcium-phosphate), the results of extracorporeal shock wave lithotripsy (eswl) therapy in 81 patients with uti and 30 patients with no sign of uti were comparatively evaluated. eswl parameters (shock wave number, kilovolt value, treatment time) and stone-free status during 3 and 6 months of follow-up were assessed for each patient, and the results were evaluated with ...	1996	8912450
phylogenetic relationships of entomopathogenic nematophilic bacteria: xenorhabdus spp. and photorhabdus sp.	phylogenetic relationships of xenorhabdus spp. and photorhabdus sp. were investigated on the basis of 16s rrna gene sequences. xenorhabdus spp. and photorhabdus sp. were grouped together with proteus vulgaris and arsenophonus nasoniae. this group was distant from other members of the family enterobacteriaceae. xenorhabdus japonicus, previously proposed as a new species, was nearly located to xenorhabdus nematophilus. signature nucleotides of x. japonicus were identified that distinguish it other ...	1996	8914266
structure of the o-specific polysaccharide chain and serological characterization of the proteus penneri 62 lipopolysaccharide compared with the lipopolysaccharides of the p. penneri strains.	the chemical structure of the o-specific polysaccharide chain of proteus penneri 62 lipopolysaccharide (lps) containing n-acetylisomuramic acid was established using acid hydrolysis, solvolysis with anhydrous hydrogen fluoride and 1h and 13c nmr spectroscopy. cross reactivity of the anti-o-serum p. penneri 62 with a number of other strains of the same species isolated in the usa, canada, germany and poland is discussed.	1996	8915524
structure of a new n-acetylisomuramic acid-containing o-specific polysaccharide of proteus penneri strains 19 and 35.	o-specific polysaccharides, together with oligosaccharide products of their degradation, were isolated by gpc after mild acid delipidation of lipopolysaccharides of proteus penneri strains 19 and 35. the polysaccharides had the same trisaccharide repeating unit containing one residue each of d-galactose, 2-acetamido-2-deoxy-d-glucose, and 2-acetamido-3-o-[(s)-1-carboxyethyl]-2-deoxy-d-glucose (n-acetylisomuramic acid). on the basis of 1d and 2d 1h and 13c nmr spectroscopy, including 2d correlati ...	1996	8916545
cytotoxic and antibacterial activities of sesquiterpene lactones isolated from tanacetum praeteritum subsp. praeteritum.	ten sesquiterpene lactones and one sesquiterpene isolated from tanacetum praeteritum subsp. praeteritum: 1 alpha, 6 alpha -dihydroxyisocostic acid methyl ester (2), 1 alpha-hydroxy-1-deoxoarglanine (3), douglanin (5), santamarin (6), reynosin (7), 1-epitatridin b (8), ludovicin a (10), armexin (12), armefolin (13), armexifolin (14), and 3 alpha-hydroxyreynosin (15) were tested against the human lung carcinoma cell line glc4 and the colorectal cancer cell line colo 320 as well as against the bact ...	1996	8923806
[enterobacteria isolated from commercial fishes from the volga-caspian basin].	enteric bacteria, such as proteus, citrobacter [correction of cytobacter], and providencia, were found to be prevalent particularly in the gills and intestines of marketable fishes of the volga and caspian seas. half of all the enteric bacteria was detected in the fish kidneys, spleen, and liver. this shows it necessary to standardize different types of fish raw materials.	1996	8925957
proteus mirabilis ambient-temperature fimbriae: cloning and nucleotide sequence of the aft gene cluster.	uropathogenic proteus mirabilis produces at least four types of fimbriae. amino acid sequences from two peptides, derived by tryptic digestion of the structural subunit of one type of these fimbriae, the ambient-temperature fimbriae, were determined: nvvpgqpsstq and liegenqlnyna. pcr primers, based on these sequences and that of the n terminus, were used to amplify a 359-bp fragment. a cosmid clone, isolated from a p. mirabilis genomic library by hybridization with the 359-bp pcr product, was us ...	1996	8926119
prevalence, antimicrobial properties and beta-lactamase production of haemolytic enterobacteria in patients with diarrhoea and urinary tract infections in legos, nigeria.	to determine the prevalence, antimicrobial properties and beta-lactamase production of haemolytic enterobacteria in patients with diarrhoea and urinary tract infections in lagos, nigeria.	1996	8771935
increased serum and synovial fluid antibodies to immunoselected peptides in patients with rheumatoid arthritis.	to investigate the role of potential immunoselected phages displaying random peptides in addition to possible antigen leads in rheumatoid arthritis (ra) by assaying the levels of synovial fluid (sf) and serum antibodies to synthetic peptides.	1996	8774161
specificity studies of bacterial sulfatases by means of structurally defined sulfated oligosaccharides isolated from shark cartilage chondroitin sulfate d.	chondro-4-sulfatase and chondro-6-sulfatase from proteus vulgaris and delta-hexuronate-2-sulfatase from flavobacterium heparinum are potentially useful tools for structural studies of chondroitin sulfate and dermatan sulfate. their substrate specificities were investigated with various structurally defined, sulfated hexasaccharides isolated from chondroitin sulfate as described in the accompanying report [sugahara, k., nadanaka, s., takeda, k. & kojima, t. (1996) eur. j. biochem. 239, 871-880]. ...	1996	8774737
structural analysis of unsaturated hexasaccharides isolated from shark cartilage chondroitin sulfate d that are substrates for the exolytic action of chondroitin abc lyase.	the enzymatic action of highly purified chondroitin abc lyase from proteus vulgaris is dependent on the size of the substrate, and the enzyme does not cleave tetrasaccharides, irrespective of their sulfation profiles [sugahara, k., shigeno, k., masuda, m., fujii, n., kurosaka, a. & takeda, k. (1994) carbohydr. res. 255, 145-163]. to characterize the enzyme action in more detail, we isolated nine sulfated hexasaccharides from commercial shark cartilage chondroitin sulfate d, after partial digesti ...	1996	8774738
detection of proteus mirabilis urease gene in urinary calculi by polymerase chain reaction.	urea-splitting microorganisms cannot always be detected by stone or urine culture in patients with infection stones. detection of genetic elements within the calculi by the polymerase chain reaction (pcr) may be a useful alternative. in this study, we assessed the usefulness of the pcr method in detecting the urease gene specific to proteus mirabilis in urinary calculi.	1996	8776618
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1994). iii. secular changes in susceptibility].	susceptibilities of enterococcus faecalis, staphylococcus aureus, citrobacter spp., enterobacter spp., escherichia coli, klebsiella spp., proteus mirabilis, pseudomonas aeruginosa and serratia spp. isolated from patients with urinary tract infections (utis) in 10 hospitals during june 1994 to may 1995 to various antimicrobial agents were compared with those in the same period of previous years according to a classification, uncomplicated utis, complicated utis without indwelling catheter, and co ...	1996	8776631
evaluation of chromagar orientation for differentiation and presumptive identification of gram-negative bacilli and enterococcus species.	a new chromogenic plate medium, chromagar orientation, was evaluated for use in the differentiation and presumptive identification of gram-negative bacilli and enterococcus species by a multipoint inoculation (replicator) technique. in this study, 1,404 gram-negative bacilli and 74 enterococcal isolates were tested on chromagar orientation. six control american type culture collection strains were also included with the testing to ensure quality control of the media. of the escherichia coli isol ...	1996	8784591
[in vitro antibacterial activity of a new parenteral penem, sulopenem].	eighty percent minimum inhibitory concentrations (mic80) of sulopenem against clinically isolated 12 to 80 strains of each of different bacteria were as follows: methicillin-susceptible staphylococcus aureus (mssa): 0.20 micrograms/ml, methicillin-resistant s. aureus (mrsa): 50 micrograms/ml, coagulase-negative staphylococci: 3.13 micrograms/ml, streptococcus pyogenes: < or = 0.013 microgram/ml, streptococcus pneumoniae: < or = 0.013 microgram/ml, beta-streptococci: 0.05 microgram/ml, enterococc ...	1996	8786624
[in vitro and in vivo antibacterial activities of sulopenem, a new penem antibiotic].	the in vitro and in vivo antibacterial activities of sulopenem, a new penem, were evaluated in comparison with imipenem (ipm), meropenem (mepm), ceftazidime (caz) and flomoxef (fmox). sulopenem had broad and potent antibacterial spectra against gram-positive and gram-negative bacteria, including enterococcus faecalis, proteus vulgaris, morganella morganii, enterobacter spp. and citrobacter freundii. sulopenem showed concentration-dependent bactericidal activities against staphylococcus aureus, e ...	1996	8786626
serum bactericidal activities and comparative pharmacokinetics of meropenem and imipenem-cilastatin.	the pharmacokinetics and serum bactericidal activities (sbas) of imipenem and meropenem were investigated in a randomized crossover study. twelve healthy male volunteers received a constant 30-min infusion of either 1 g of imipenem plus 1 g of cilastatin or 1 g of meropenem. the concentrations of the drugs in serum and urine were determined by bioassay and high-pressure liquid chromatography. pharmacokinetic parameters were based on an open two-compartment model and a noncompartmental technique. ...	1996	8787889
a survey of sixty turkey flocks exhibiting hepatic foci taken at time of processing.	fifty turkey flocks including 24 16-week-old male flocks and 26 20-week-old male flocks were sampled at time of processing. hepatic foci were cultured for aerobic and anaerobic bacteria. the majority of these did not have any bacteria recovered from the lesions. of the bacteria that were recovered, most were facultative anaerobes, with escherichia coli and salmonella sp. comprising the most common isolates. all of the birds examined (300 total) for parasites were infected with varying levels of ...	1996	8790901
the structure of the o-specific polysaccharide of proteus penneri 52.	an acidic o-specific polysaccharide isolated from proteus penneri 52 contains d-galacturonic acid, 2-acetamido-2-deoxy-d-glucose, and 2-acetamido-2-deoxy-d-galactose in the ratio 1:2:2. on the basis of sugar analysis and nmr spectroscopy, which included one-dimensional tocsy, two-dimensional cosy, heteronuclear 13c, 1h-cosy, and rotating-frame noe spectroscopy, the following structure of the pentasaccharide repeating unit of the o-specific polysaccharide was established: [sequence: see text] cro ...	1996	8797860
sonographically guided aspiration: an alternative therapy for tubo-ovarian abscess.	our objective was to evaluate the role of single-step ultrasound-guided aspiration in conjunction with intracavitary antibiotic instillation for the treatment of tubo-ovarian abscess. ten women with the clinical and sonographic diagnosis of tubo-ovarian abscess, who failed to respond to systemic antibiotic therapy, were treated by ultrasound-guided aspiration of the abscess followed by intracavitary instillation of a combination of antibiotics. all ten women improved clinically and none required ...	1996	8807762
[in vitro activity of a new fluoroquinolone, marbofloxacin (ro 09-1168) against strictly anaerobic bacteria and some bacteria from human fecal flora].	as recommended by the eec, the in vitro activity of a veterinary quinolone has to be evaluated on human strains isolated from the gut and especially against anaerobes. thus, the mics for marbofloxacin were determined by the reference agar method (norma nccls m11 a3) using wilkins chalgren medium for the 124 anaerobic strains and mueller hinton agar for e. coli, proteus, enterococcus and lactobacillus. on the whole aerobes, the activity of marbofloxacin was equal or slightly greater to that of of ...	1996	8758470
molecular cloning and overexpression of a glutathione transferase gene from proteus mirabilis.	the structural gene of the proteus mirabilis glutathione transferase gstb1-1 (gstb) has been isolated from genomic dna. a nucleotide sequence determination of gstb predicted a translational product of 203 amino acid residues, perfectly matching the sequence of the previously purified protein [mignogna, allocati, aceto, piccolomini, di ilio, barra and martini (1993) eur. j. biochem. 211, 421-425]. the p. mirabilis gst sequence revealed 56% identity with the escherichia coli gst at dna level and 5 ...	1996	8761466
study of a melanic pigment of proteus mirabilis.	the present study sought to determine whether the pigment produced by proteus mirabilis from the l-forms of various aromatic amino acids under aerobic conditions is melanic in nature. it is a black-brown pigment which behaves like a melanin in many respects, namely solubility features, bleaching by oxidizing agents and positive response to the fontana-masson assay. in the present study, for the first time, it was shown by electron spin resonance analysis that a bacterial melanin is able to act a ...	1996	8761735
douglas-fir root-associated microorganisms with inhibitory activity towards fungal plant pathogens and human bacterial pathogens.	a microbial culture collection composed of 1820 bacterial strains, including 298 actinomycete strains, was established from the roots of douglas-fir (pseudotsuga menziesii (mirb.) franco) seedlings harvested from conifer nurseries and forest sites. two hundred and thirty-four strains inhibited the growth of fusarium, cylindrocarpon, and (or) pythium spp. in in vitro assays. a significantly greater proportion of bacterial strains from actinomycete genera exhibited antifungal properties compared w ...	1996	8764683
[urinary infection in urolithiasis patients in the epirus district (northeastern greece)].	the relationship between renal stones and urinary tract infection is frequent but not well-known. in this study, urinary tract infection was found in 12% of renal stone formers. it is four times more common in females than in males. urea splitting bacteria (proteus, klebsiella, staphylococcus and pyocyaneus) lead to stone formation. they were identified in 72% of cases. proteus was predominant and the organism most frequently found in staghorn stone formers. other non urea-splitting bacteria (e. ...	1996	8766147
comparative evaluation of orally active antibiotics against community-acquired pathogens: results of eight european countries.	in this multicenter study conducted in eight european countries, 13,173 pathogens--all isolated from community-acquired infections in 1992 and 1993--were evaluated for their susceptibility to the following orally active antibiotics: penicillin g, ampicillin, amoxycillin plus clavulanic acid, cefaclor, cefuroxime, cefetamet, doxycycline and erythromycin. ten centers in italy, five in germany, in the netherlands and switzerland, four in greece and spain, three in hungary and one in finland contrib ...	1996	8751263
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1994). i. susceptibility distribution].	the frequencies of isolation and susceptibilities to antimicrobial agents were investigated on 567 bacterial strains isolated from patients with urinary tract infections in 10 hospitals during the period of june 1994 to may 1995. of the above total bacterial isolates, gram-positive bacteria accounted for 26.8% and a majority of them were enterococcus faecalis. gram-negative bacteria accounted for 73.2% and most of them were escherichia coli. 1. enterococcus faecalis. ampicillin (abpc) and imipen ...	1996	8752862
[comparative studies on activities of antimicrobial agents against causative organisms isolated from patients with urinary tract infections (1994). ii. background of patients].	clinical background was investigated on 628 bacterial strains isolated from patients with urinary tract infections (utis) in 10 hospitals during period from june, 1994 to may, 1995. 1. distributions of sex, age and urinary tract infections among over sixties males, the majority was taken by complicated urinary tract infections. among females, the uncomplicated urinary tract infections was most frequent without a relation of age. as for over 40 females, the increase of complicated uti was admitte ...	1996	8752863
[structure of the o-specific polysaccharide from proteus mirabilis o30].	based on acid hydrolysis, methylation, and one- and two-dimensional 1h- and 13c-nmr spectroscopy, including homonuclear and 1h, 13c heteronuclear correlation spectroscopy (cosy) and rotating-frame nuclear overhauser effect spectroscopy (roesy); it was found that the o-specific polysaccharide chain of the lipopolysaccharide of the bacterium proteus mirabilis o30 is a hexosamino-glucuronan built up of tetrasaccharide repeating units having the following structure: [formula: see text] the degree of ...	1996	8754267
concentrations of ceftriaxone (1,000 milligrams intravenously) in abdominal tissues during open prostatectomy.	ceftriaxone concentrations in abdominal tissues were evaluated at different stages of open prostatectomy. ceftriaxone was administered as antibiotic prophylaxis, and 15 consecutive patients were given a single dose of ceftriaxone (1,000 mg intravenously in 1 min) 30 min before surgery. ceftriaxone concentrations in tissue were determined at three stages of the surgical procedure; upon the opening of the abdominal cavity, during the prostatectomy, and upon the closure of the abdominal cavity. sam ...	1996	8723493
an abietane diterpene and two phenolics from salvia forskahlei.	from the roots of salvia forskahlei a new diterpenoid, forskalinone, two new aromatic compounds, the octanol esters of cis- and trans-4-o-methyl-caffeic acid dimers, were isolated together with the known compounds stigmast-3-one, sitosterol and alpha-amyrin. the structures of the new and the known compounds were established by spectral data. the antimicrobial activity of forskalinone and the dimeric cinnamic acid esters was tested against standard bacterial strains and a yeast, namely bacillus s ...	1996	8728063
structural and immunochemical studies on the lipopolysaccharide of the 't-antigen'-containing mutant proteus mirabilis r14/1959.	in doc-page, lipopolysaccharide (lps) of proteus mirabilis r14/1959 (rb-type) mutant showed a ladder-like migration pattern indicating the presence of a high molecular weight polysaccharide chain. the isolated polysaccharide, called t-antigen because of similarity with the t1 chain of salmonella friedenau lps, contained d-glucose, d-galacturonic acid (d-gala), and d-glcnac in molar ratios 2:1:1 and was structurally different from the o-antigen of the parental s-strain p. mirabilis s1959 but iden ...	1996	8731019
effect of aerobic bacteriology on the clinical presentation and treatment results of chronic suppurative otitis media.	the effect of aerobic bacteriology on the clinical presentation, complications of the disease and long-term results of surgical treatment was assessed in a cohort of 368 patients with chronic suppurative otitis media. bacteriological findings showed no significant difference between child and adult patients. staphylococcus aureus was isolated in cholesteatoma ears more frequently than pseudomonas aeruginosa, in chronic ears without cholesteatoma the situation was reversed. bacteriological findin ...	1996	8733450
glycerol and ichthammol: medicinal solution or mythical potion?	glycerol and ichthammol (g & i) has been used for generations by otologists. however, there is a paucity of information on both its mode of action and its anti-bacterial properties. the aim of this paper was to ascertain firstly, what the most common organisms found in discharging ears were and secondly, what antibacterial activity g & i had against these organisms. all ear swabs from 1992-1994 in our unit were reviewed to ascertain the prevalence of the commonly isolated organisms. fresh isolat ...	1996	8733451
antimicrobial activity of paliurus spina-christi mill. (christ's thorn).		1996	8735457
lymphangiolipoma of the thoracic spine in a pediatric patient with proteus syndrome.	proteus syndrome is a rare hamartomatous disorder involving macrodactyly, hemihypertrophy, and subcutaneous lymphangiomas; fewer than 25 cases have been reported worldwide. we report a case of a thoracic epidural lymphangiolipoma in a 5-year-old boy with proteus syndrome. computerized axial tomography (ct) of the thoracic spine revealed a left posterior mediastinal mass that extended into the spinal canal through adjacent neural foramina. no sign of spinal cord compression was observed despite t ...	1996	8739411
potential contribution of optional urease-positive bacteria to idiopathic urinary calcium stone formation. ii. microlith formation kinetics in a fermenter model of the urinary tract infected by optional urease-positive microorganisms.	we investigated the effects of weak to moderate urease hydrolysis by optional urease-positive microorganisms in an artificial urine model enriched with calcium phosphate and calcium oxalate in respect of calcium stone formation. the incubation experiments were performed using a discontinuously running fermenter device to simulate the urinary system. the kinetics of cell division rates, ph and ammonium ion production were measured and correlated to crystallite appearance in the incubation medium. ...	1996	8740975
distribution of diaminopropane and acetylspermidine in enterobacteriaceae.	polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ...	1996	8742354
an evaluation of the in vitro activity of piperacillin/tazobactam.	tazobactam is a new, irreversible inhibitor of bacterial beta-lactamases of staphylococci, plasmid-mediated beta-lactamases of the tem and shv types found in escherichia coli and klebsiella species and beta-lactamases of anerobes such as bacteroides species. its combination with piperacillin, a broad spectrum ureido-penicillin, would be expected to improve the activity of piperacillin against staphylococci, tem and shv beta-lactamase producing gram negative bacteria and anerobes. minimal inhibit ...	1996	8743825
production of superoxide dismutases from proteus mirabilis and proteus vulgaris.	proteus mirabilis and proteus vulgaris expressed a combination of superoxide dismutase (sod) activities, which was assigned to fesod1, fesod2 and mnsod for p. mirabilis, and fesod, mnsod and cuznsod for p. vulgaris. production of the sod proteins was dependent on the availability of iron, whether cells were grown under anaerobiosis or aerobiosis and growth phase. nalidixic acid and chloramphenicol inhibited cell growth and the iron- and dioxygen-dependent production of sod. these results support ...	1996	8744895
[determination of radioresistance of microbes of different species irradiated separately and jointly].	radioresistance of lactose positive and lactose negative esch. coli, lactose positive esch. coli and proteus, yellow sarcina and white staphylococcus, white and pink yeasts, white and yellow staphylococcus was studied after irradiation and seeding on hard media using separate and combined methods. in most cases radioresistance of the microbes did not depend of exposure conditions. however, lactose positive esch. coli irradiated with proteus increased proteus radioresistance to its own standard. ...	1996	8696486
influence of fowl uropygial gland and its secretory lipid components on growth of skin surface bacteria of fowl.	bacterial species, which occur on the breast skin surface of adult (1 year old) white leghorn fowl with intact uropygial gland, were identified as : staphylococcus epidermidis, sarcina lutea, streptomyces sp. and a facultative diphtheroid belonging to the genus corynebacterium; s. epidermidis being the most predominant one. two species of bacteria, namely, staphylococcus aureus and proteus sp. were shown to colonize the skin surface after 60 days of captivity. extirpation of uropygial gland caus ...	1996	8698407
isolation and characterisation of dog uropathogenic proteus mirabilis strains.	proteus mirabilis strains isolated from the urine of dogs with urinary tract infections, were characterised with respect to the production of haemolysin and fimbriae. in contrast to healthy dogs, p. mirabilis was also isolated in high numbers from the faeces of dogs suffering from recurrent urinary tract infections. production of fimbriae was demonstrated by electron microscopy and the presence of genes for two different types of major fimbrial subunits (mr/p-like or uca-like) was demonstrated b ...	1996	8701578
[the immune reactivity of patients with chronic bronchitis on vaccinal therapy].	the treatment of 17 patients having chronic obstructive bronchitis with a combined vaccine resulted in a rise in the number of t lymphocytes and t suppressors, as well as a simultaneous increase in their functional activity, in these patients. moreover, an increase in the ingestive activity of neutrophils and in the concentration of immune complexes was registered. treatment with the vaccine containing staphylococcus aureus, klebsiella pneumoniae, proteus and escherichia coli antigens facilitate ...	1996	8701661
antimicrobial activity of a 13 amino acid tryptophan-rich peptide derived from a putative porcine precursor protein of a novel family of antibacterial peptides.	it has long been speculated that porcine cathelin is an n-terminal fragment of a longer precursor protein which possesses antimicrobial activity. in an attempt to find such a precursor, a cdna clone was recently isolated and sequenced by screening a cdna library from porcine bone marrow. in order to identify the functional activity of the putative protein encoded by an open reading frame, we have synthesized various lengths of peptides that correspond to the c-terminal region of the protein and ...	1996	8706838
abnormal phagocytic function in children under one year of age.	the action of white blood cells (wbc) against proteus mirabilis was assayed in 22 children following meningitis or septicaemia and compared with that in control groups of: (1) adult volunteers, (2) children undergoing minor surgical procedures for non-infective conditions, and (3) children being investigated for suspected renal disease. wbc bacterial killing tests were normal in all 55 adult volunteers. abnormal results were obtained in 11 of 22 (50%) children with meningitis or septicaemia, 10 ...	1996	8708365
ticarcillin/clavulanic acid: determination of minimal inhibitory concentrations against bacterial strains isolated from patients in intensive care units. comparison with other agents.	a total of 303 bacterial strains isolated from bronchoaspirates of intensive care unit (icu) patients, collected through june and december 1993, were tested for susceptibility to ticarcillin/clavulanic acid, imipenem, amikacin, ceftazidime, ciprofloxacin and piperacillin. the minimal inhibitory concentration (mic) for each antibiotic was determined according to the nccls, by means of serial dilution on microplates. the isolates, 80.8% of which were beta-lactamase producing strains, belonged to p ...	1996	8708742
piperacillin/tazobactam in the treatment of hospitalized patients with urinary tract infections: an open non-comparative and multicentered trial.	the aim of this multicentered, prospective and open study was to determine the clinical and bacteriological efficacy and safety of piperacillin/tazobactam (4g/500 mg iv tid) in the treatment of 79 adult patients with complicated urinary tract infections (uti) requiring hospitalization. forty-seven women and 32 men (mean age 54.2 years, and range 21-91) from 4 argentinean and 6 mexican hospitals were enrolled. sixty-one clinically and bacteriologically evaluable patients were treated for a mean o ...	1996	8708743
differentiation of proteus mirabilis and proteus vulgaris strains by means of proticine typing: a longitudinal epidemiological study.	in the years 1979, 1980, 1982-83, 1986-87 and 1992-93, 673 strains of p. mirabilis and 25 strains of p. vulgaris were isolated from the urinary tracts of patients at a teaching hospital in brno. in 1982-83 and 1992-93, strains of p. mirabilis and p. vulgaris were isolated from the urine and faeces of two groups of brno population and used as controls. using the p/s typing method, 94.7% of hospital isolates and 85.5% of control strains could be differentiated by their types. the strains that coul ...	1996	8996720
screening of in vitro antibacterial activity from syzygium guineense (willd) hydrosoluble dry extract.	diarrhoea is one of the most important causes of infant mortality in the world. as modern drugs are expensive or unavailable in developing countries, many people use traditional medicines in africa for the treatment of several diseases. in our study, we investigated the antibacterial activity of syzygium guineense extract in order to assess its activity on some bacterial strains involved in diarrhoeal diseases and to justify its use. the aqueous dry extract was prepared by decoction followed by ...	1996	9008903
comparison of serological reactions of rickettsiae-infected patients and rabbit anti-proteus ox antibodies with proteus ox2, ox19 and oxk lipopolysaccharides.	the reactivity of anti-rickettsiae human antibodies with proteus ox cells is used as a presumptive rickettsial diseases diagnostic test weil-felix reaction. in presented studies we compare the reactivity of human anti-rickettsiae and rabbit anti-proteus antibodies with series of proteus ox2, ox19 and oxk lipopolysaccharides (lps). polyclonal rabbit anti-ox2, anti-ox19 and anti-oxk sera reacted only with the homologous lps--ox2, ox19 and oxk, respectively. the antibodies of japanese spotted fever ...	1996	9017163
glutamate dehydrogenase antigen detection in plasmodium falciparum infections.	the usefulness of malaria diagnosis by plasmodium falciparum gdh (nadp+), obtained by affinity chromatography, is demonstrated in elisa assays, testing igg antibodies against gdh (nadp+) from patients with acute malaria, who have had two or more episodes of malaria, or from sera of hyperimmune patients. gdh (nadp+) thermal stability was demonstrated in a high heat resistance assay. the immunofluorescence assay demonstrated that anti-culture (p. falciparum) supernatant serum and anti-gdh (nadp+) ...	1996	9017909
the small intestine as the origin of bacteremia in acute diffuse peritonitis.	studies have demonstrated the failure of gut barrier function in all cases of experimental acute diffuse peritonitis. histobacterioscopy and electron microscopy showed the occurrence of bacteria under the basal membrane, in lymphatic and blood capillaries of the small intestinal villi. experimental and clinical trials with blood sampling from different regions of the circulation have demonstrated the gut origin polymicrobial bacteremia in 66% of patients and in 75% of experimental animals with a ...	1996	9025333
[the value of bacteriologic examination in cataract surgery].	the purpose of our study was to compare the incidence of intrabulbar infections following cataract extraction and its correlation with the results of preoperative bacterial cultures. we examined 2344 patients who were operated on from 1st january 1993 to 31st december 1994 in our hospital. patients were divided into 3 groups. in the first group surgery, was performed only in cases where preoperative cultures were negative, in the second group conjunctival cultures were prepared just before surge ...	1996	9026569
[influence of vapours of paint and toxic dusts on mucous membranes of the upper airways in paint and varnish factory workers].	macroscopic, cytologic and bacteriologic conditions of mucous membranes of the upper airways in workers (n = 146) of the polifarb factory, wrocław, exposed to dusts and solvent vapours used in the paint and varnish production was estimated. in 89% of the workers, pathologic changes in throat mucous membranes were observed. three types of macroscopic changes were distinguished. in workers with the shortest period of employment, laryngeal oedema congestion alteration was diagnosed, and atrophic ch ...	1996	9026624
[the development of a proteus mirabilis macrocolony on a solid nutrient medium].	as revealed in this study, the macrocolony of proteus mirabilis, formed on solid culture medium, may consist both of the main part and of sporadically appearing dissociating subcolonies, considerably differing one from another. the outer edge of the main part of the macrocolony of swarming cells is represented by bacteria located in three perpendicular directions. the next intermediate area consisting of two layers is represented by bacteria oriented, as a rule, in one direction. the center of t ...	1996	9027169
[cytolysins and homologous proteins produced by gram-negative bacteria. ii. hemolysins of aeromonas, serratia, proteus and morganella and proteins from gram-negative bacteria related to cytolysins].	this article presents the genetic determination, synthesis and activation of hemolysins produced by mentioned above bacteria, as well as the possible role of these toxins in the pathogenicity. all these cytolysins are related to rtx proteins presented to the previous paper. the short information about cytotoxic proteins from other gram-negative bacteria (p. aeruginosa, k. pneumoniae, vibrionaceae, g. vaginalis) and homologous proteins from e. chrysanthemi and r. leguminosarum biovar viciae are a ...	1996	9019745
the effect of positive semen bacterial and ureaplasma cultures on in-vitro fertilization success.	a total of 342 couples planning to undergo in-vitro fertilization (ivf) were examined for the presence of bacteria in semen prior to and during the procedure. pregnancy rates were analysed retrospectively to ascertain any adverse affects if > 10000 colony forming units (cfu)/ml bacteria were detected in the semen sample. the most common bacteria isolated from semen were enterococcus spp. (73%). the presence of these bacteria did not affect the pregnancy rate of the patients with positive culture ...	1996	9021392
[antimicrobial activities of cefetamet against clinical isolates from urinary tract infection].	in order to evaluate antimicrobial activity of cefetamet (cemt), minimum inhibitory concentrations (mics) of cemt and control drugs were determined against gram-negative rods mainly from complicated urinary tract infections examined in our laboratory from april to september of 1994. the results are summarized as follows; 1. the obtained strains were citrobacter diversus 20, citrobacter freundii 30, enterobacter aerogenes 20, enterobacter cloacae 30, serratia marcescens 30, proteus mirabilis 30, ...	1996	9032594
[expression of synthetic gene for human angiogenin in e. coli cells].	a synthetic gene for human angiogenin was cloned into prtang under control of the proteus mirabilis reca promoter. after induction with mitomycin c, escherichia coli cells transformed with prtang produced an additional 14.2-kda protein. according to electrophoretic and immunochemical analyses, this protein was identical to human angiogenin.	1996	9054338
the associated microflora to the larvae of human bot fly dermatobia hominis l. jr. (diptera: cuterebridae) and its furuncular lesions in cattle.	the microflora associated to furuncular lesions, larvae and pupae of dermatobia hominis, as well as the relationships between parasite, host and microflora associated, as a comprehensive microsystem, has been studied. one hundred and two furuncular myiasis due to d. hominis larvae in several breeds of cattle were studied and the following bacterial species were significant: staphylococcus aureus, s. epidermidis, s. warneri, bacillus subtilis and escherichia coli. closely related, the microflora ...	1996	9040848
[fluoroquinolone and aminoglycoside resistance in chromosomal cephalosporinase-overproducing gram-negative bacilli strains with inducible beta-lactamase].	the increasing prevalence of stable derepressed mutants over-producers of type i chromosomal cephalosporinase in inducible enterobacteriaceae and pseudomonas aeruginosa challenges the adequacy of third generation cephalosporins in the empirical treatment of certain nosocomial infections. we sought to determine the frequency of stable over-producers of type i enzyme and their associated resistance to fluoroquinolones and aminoglycosides.	1996	9044634
[evaluation of chromogenic medium cps id2 (biomérieux) in urine cultures].	the aim of the study was to evaluate the chromogenic agar plate cps id2 (biomérieux) and determine its cost-benefit ratio.	1996	9044635
[a case for diagnosis: proteus syndrome].		1996	9033725
[aids manifestations in otorhinolaryngology].	the otorhinolaryngological signs of aids are reviewed (both analysis and synthesis) following the chronological order of the literature. the earliest clinical pictures, their frequency and time of onset, are described by the authors studied. in 1986 the ent signs of this disease were well known, and in our region the same multiple, polyfacetic aspects are seen. personal experience of this is described, emphasizing how seldom the diagnosis has been made, except early on. usually the cases seen an ...	1996	9064187
[proteus lipopolysaccharides].		1996	9072758
[serologic studies of cross reactions between strains of proteus mirabilis oxk (pro 10/52) and proteus mirabilis s1959].	strong cross-reactions are described between proteus mirabilis strains having the same structures of repeating units of their o-specific polysaccharides. these strains are used in routine diagnosis of rickettsiae.	1996	9072766
[serologic cross reactions between strains of proteus mirabilis belonging to serogroup o43 i o10].	in this work an epitope has been determined on lipopolysaccharide of proteus mirabilis o43. this epitope is recognized by specific antibodies.	1996	9072767
acquired tracheomalacia--a case report.	tracheomalacia is a condition characterized by weakness of the tracheal walls and supporting cartilage. it may be congenital, occurring in association with other lesions, such as vascular ring, tracheoesophageal fistula, dyschondroplasia, congenital cysts or tumors, or it may be acquired, most commonly as a result of protracted endotracheal intubation and less often of irradiation, trauma or neoplasm. we present a case of acquired tracheomalacia in a 15-year-old boy, a victim of proteus syndrome ...	1996	9084553
prevalence of pathogenic role of morganella-proteus providencia-group of bacteria in human faeces.	speculation in literature that organisms of the morganella-proteus-providencia (mpp) group can cause diarrhoea led to the present studies which investigated their occurrence and pathogenic role in human faeces. faecal specimens were collected from a total of 307 subjects, 80 of which were from diarrhoea cases and 277 from healthy controls. ninety-two species of the mpp (24/80 diarrhoea and 68/277 control) were isolated from 30% of all subjects. none of the species of the mpp group was significan ...	1996	9110049
[the role of pseudomonas aeruginosa and proteus in the etiology of suppurative surgical infection].		1996	9138809
[the validation of empirical emergency antibacterial therapy in acute pyelonephritis].	as many as 254 patients with acute pyelonephritis were evaluated for the whole range of possible inflammatory process pathogens with the aid of automatic microbiological system [symbol: see text]. urine from the urinary bladder, renal pelves (as directed) was studied as was biopsy specimen of the kidney. analysis of species range of the identified organisms showed escherichia coli, proteus, and mycoplasma, enterobacter, coccal flora and pseudomonads to be in 27.9%, 15.9%, 14.9%, 8.6%, and 5.5% r ...	1996	9138828
preoperative treatment of children with chronic suppurative otitis media.	259 patients with chronic suppurative otitis media were bacteriologically assessed. 12 bacterial strains were isolated in 196 of the cases. the most common species present was staphylococcus - 77 (39%) cases, followed by proteus vulgaris isolated in 36 (18%) cases, and pseudomonas aeruginosa in 33 (17%) cases. the flora in 25 cases (18%) yielded two bacterial strains with identical oxygen demands, and a mixture of staphylococcus and streptococcus in 32%. in 60.6% of cases the flora was sensitive ...	1996	9145593
the role of o-acetylation in the metabolism of peptidoglycan in providencia stuartii.	the gentamicin 2'-n-acetyltransferase [ec 2.3.1.59; aac(2')-ia] of providencia stuartii was shown to contribute to the o-acetylation of peptidoglycan and mutants that either under- or overexpress the aac(2')-ia gene was characterized phenotypically to possess either lower or higher levels of peptidoglycan o-acetylation, respectively, compared to the wild-type. these mutants were subjected to scanning electron microscopy. p. stuartii pr100, with 42-44% peptidoglycan o-acetylation compared to 54% ...	1996	9158736
antagonistic activity of lactobacillus acidophilus fermented milk against different pathogenic bacteria.	lactobacillus acidophilus strains tested showed inhibitory activity towards salmonella typhi, staphylococcus aureus, escherichia coli, proteus vulgaris and yersinia enterocolitica. however, wide variations in the activity of the strains were observed. antagonistic activity of the strains exhibited high heat stability (120 min at 92 degrees c, 15 min at 121 degrees c) but it was markedly influenced by changes in ph. effect of kind of milk on antagonistic activity was variable, whereas skimming ha ...	1996	9246920
an in-vitro study of smear layer removal and microbial leakage along root-canal fillings.	the objective of this study was to determine the effect of removal of the smear layer on canal obturation as measured by penetration of bacteria from a coronal direction. fifty-four extracted human teeth were decoronated and instrumented in a uniform manner. following instrumentation the root canals of 20 teeth were rinsed with 17% edta and 5.25% naocl to remove the smear layer before obturation. a second group of 20 teeth were flushed with naocl alone. the teeth of both groups were obturated wi ...	1996	9206432
[etiological investigation on diarrhoeal diseases in the rural area of suixian county, yu dong plain in china].	to study the etiology of diarrhoeal diseases in the rural area, we carried out a study on diarrhoeal diseases in two natural villages. 15 kinds of diarrhoeal pathogens including 172 strains were isolated from 318 patients with diarrhoea making the isolation rate 49.37%; 12 kinds of diarrhoeal pathogens and 69 strains were isolated from 310 healthy controls and the isolation rate was 22.26%; 6 kinds of diarrhoeal pathogens and 44 strains were isolated in 405 samples obtained from the envronmenfal ...	1996	9387573
[the evaluation of cefmetazole in clinical use. the study group of cefmetazole in clinical use].	to study the clinical effect of cefmetazole (cmz), we treated with cmz 1,926 patients with various infections involving the respiratory tract, gastrointestinal tract, liver and biliary tract, skin and soft tissue and others. the marked effect rate was 56.5%, and the effect rate 33.7%. the sensitivity test of cmz was also performed in vitro. staphylococcus aureus, streptococcus, staphylococcus epidermis, e. coli, s. typhi, b. salmonella, b. shigella, b. klebsiella pneumoniae, b. proteus, etc. wer ...	1996	9592326
crystallization and preliminary x-ray analysis of a bacterial glutathione transferase.	crystals of a bacterial glutathione s-transferase from proteus mirabilis have been grown from polyethylene glycol by the hanging-drop vapour-diffusion method. successful crystallization required the presence of the substrate glutathione. the crystals belong to the tetragonal space group p4 with cell dimensions a = b = 90.9 and c = 117.3 a. they contain between three and six monomers in the asymmetric unit and diffract to beyond 2.3 a resolution.	1996	15299741
inhibitory effects of secondary metabolites from the red alga delisea pulchra on swarming motility of proteus mirabilis.	abnormal, uncoordinated swarming motility of the opportunistic human pathogen proteus mirabilis was seen when a crude extract of the australian red alga delisea pulchra was added to the medium. this occurred at concentrations at which growth rate, swimming motility, cell elongation, polynucleation, and hyperflagellation were not affected. one halogenated furanone from d. pulchra inhibited swarming motility at concentrations that did not affect growth rate and swimming motility. other structurall ...	1996	16535454
[treatment of pediatric outpatients with complicated urinary tract infections].	in an open and prospective study involving outpatient children with complicated urinary tract infections (uti), we evaluated the therapeutic efficacy of ceftriaxone administered intramuscularly, once-daily--50 to 70 mg/kg, during 8 to 10 days. initially, the selected patients exhibited at least two of the following clinical criteria: age below 6 months, any degree of toxicity, fever, strong suspicion or proved abnormalities of their urinary tracts and lumbar pain in children older than 4 years. ...	1996	14688917
a thin liquid film thickness shear mode bulk acoustic wave sensor for determination of proteus mirabilis.	a thickness shear mode bulk acoustic wave sensor coated with a thin liquid culture medium film was developed and applied to determine the concentration of proteus mirabilis (p. mirabilis). experiments demonstrated that there was a good linear relationship between the turning point time and the logarithm of the p. mirabilis concentration in the range 2.0 x 10(2)-2.0 x 10(6) cells ml(-1). the detection was fast and accurate because of the sharp turning point of the response due to the thin culture ...	1996	18966534
screening of some food poisoning bacteria in sausage and hamburger meat.	microbial investigation of 30 samples of sausage meat and 30 samples of hamburger meat, freshly prepared, were collected randomly from different markets in alexandria city, to determine the bacteriological status of these products. the average counts/gm of total bacteria, total coliforms and fecal coliform in sausage meat were 1.33 x 10(7), 9.16 x 10(5) and 1.11 x 10(3) respectively, and in hamburger samples were 8.47 x 10(5), 2.63 x 10(3) and 1.35 x 10(3) respectively. shigella, escherichia col ...	1996	17217001
ureaplasma urealyticum as a causative organism of urinary tract infection stones.	ureaplasma urealyticum is a fastidious organism which is not recovered by conventional bacterial cultures techniques, but special cultures are required for its isolation and identification. as it is a urease-producing organism, it is considered a risk factor for the formation of struvite calculi in the urinary tract. a total of 30 patients with urinary infection stones (19 of them with the 1st formation and 11 with recurrent stone formation) were included in the study. both bladder urine specime ...	1996	17217015
urinary tract infection in patients with short-term indwelling.	seventy patients (44(62.9%) females) requiring short-term urinary bladder catheterisation at the kenyatta national hospital, nairobi formed the study population. their mean ages +/- sd was 41+/- 26 years (range 13-100 years). the common indications and objectives for catheterisation included keeping the environment dry (41.1%), relieving urinary retention (27.0%) and urinary incontinence (24.3%). urinary tract infection (uti) was documented in 48 (68.6%) of the patients. the commonest infection ...	1996	17451307
comparative in vitro activity of l-ofloxacin and fk037 to other agents against 10,040 fresh clinical isolates.	fresh clinical isolates collected from november 1, 1992 through november 1, 1993, were tested by agar dilution against 26 different antimicrobial agents including fk037 and l-ofloxacin. among the 10 040 organisms tested were staphylococcus aureus (n = 1222), methicillin-resistant staphylococcus aureus (mrsa, n = 455), staphylococcus epidermidis (n = 533), staphylococcus hominis (n = 90), staphylococcus hemolyticus (n = 89), streptococcus pneumoniae (n = 144), escherichia coli (n = 2326), klebsie ...	1996	18611710
health studies on the indian house crow (corvus splendens).	fifteen indian house crows (corvus splendens) were obtained from a live-trap in dar es salaam, tanzania. two died soon after arrival. the remainder were examined clinically prior to euthanasia. all birds were examined post-mortem and a limited number of laboratory investigations was performed. none of the birds showed significant clinical signs or pathological lesions. lice were found on one. only escherichia coli and proteus spp. were isolated from the rectum. coccidia were detected in three bi ...	1996	18645865
bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards.	a survey was carried out to investigate the aerobic bacterial flora of captive houbara (chlamydotis undulata), kori (ardeotis kori) and rufous-crested (eupodotis ruficrista) bustards maintained at the national avian research center, abu dhabi, united arab emirates. none of the houbara or rufous-crested bustards sampled were bred in captivity by the center but four of the kori bustards sampled were captive-bred. faecal, cloacal, oropharyngeal and skin samples (from under the wing) from clinically ...	1996	18645872
cache write generate for parallel image processing on shared memory architectures.	we investigate cache write generate, our cache mode invention. we demonstrate that for parallel image processing applications, the new mode improves main memory bandwidth, cpu efficiency, cache hits, and cache latency. we use register level simulations validated by the uw-proteus system. many memory, cache, and processor configurations are evaluated.	1996	18285208
medical microbiology	the gram-negative bacilli of the genera escherichia, klebsiella, enterobacter, serratia, citrobacter,and proteus(table 26- 1) are members of the normal intestinal flora of humans and animals and may be isolated from a variety of environmental sources. with the exception of proteus, they are sometimes collectively referred to as the coliform bacilli because of shared properties, particularly the ability of most sp ...	1996	21413290
proteus syndrome and distal arthrogryposis.		1997	9457508
[the results of experimental and clinical studies of a multicomponent vaccine made of the antigens from opportunistic microorganisms].		1997	9460881
[neurophysiological aspects of proteus syndrome].	the proteus syndrome was defined in 1983 by wiedeman. however, the first case mentioned in the literature was that of joseph merrick, the elephant man, presented by sir frederick treves in 1884. it is a rare pathological condition. its multiple clinical features include; partial gigantism of hands and/or feet, pigmented nevi, hemihypertrophy of the body, tumors, skeletal anomalies, growth disorders and visceral anomalies. hereditary transmission has not been clearly defined. diagnosis and treatm ...	1997	9462983
studies on antimicrobial effect of the antihistaminic phenothiazine trimeprazine tartrate.	the antibacterial and bactericidal activities of the antihistamine trimeprazine were studied against 243 strains of bacteria which included both gram positive and gram negative types. the susceptibility of these bacterial strains to trimeprazine was assessed by determining their minimum inhibitory concentration (mic) which was found to be between 10 and 100 micrograms/ml. nineteen strains of staphylococcus spp. and salmonella spp. were trimeprazine. most of the strains belonging to bacillus spp. ...	1997	9468728
structural analysis of compound i in hemoproteins: study on proteus mirabilis catalase.	ferryl catalysis has attracted considerable interest, because a diverse variety of enzymes use ferryl intermediates to perform difficult chemistry. the structure of the reactional intermediate compound i of proteus mirabilis catalase (pmc) has been solved using time-resolved x-ray diffraction techniques and single crystal microspectrophotometry. formation of compound i is characterized by significant changes in the absorbance spectrum, and the creation of an oxoferryl group on the distal side of ...	1997	9479449
[treatment of lower respiratory tract infections in the elderly].	sixty outpatients at the age of 65 to 75 years with exacerbated chronic bronchitis were treated with antibiotics: amoxycillin/clavulanic acid (20 patients), cefaclor (20 patients) and ciprofloxacin (20 patients). the treatment course in all the cases was 5 days. bacteriological tests of the sputum specimens and estimation of the isolate antibiotic susceptibility by the disk diffusion method were applied to all the patients before and after the treatment. 73 per cent of the patients had mixed inf ...	1997	9480649