Publications

the use of visual feedback to control vocal intensity and nasalization.	a device which provides visual feedback of vocal intensity, nasalization, or a ratio of nasalization to vocal intensity, is described. the device was used in several experiments in which control of vocal intensity was a necessary component. normal and hypernasal speakers were able to control their vocal intensities successfully when using the visual display. the display also was used in an experiment designed to determine how well normal speakers can manipulate their nasalization without visual ...	1999	89126
use of permanent hair dyes and cancer in registered nurses.		1999	89538
inosine analogs as substrates for adenosine kinase--influence of ionization of the n-1 proton on the rate of phosphorylation.		1999	23125
n-acetylprocainamide pharmacokinetics in functionally anephric patients before and after perturbation by hemodialysis.	napa pharmacokinetics were studied in 6 functionally anephric patients. distribution and nonrenal elimination of this drug were found to be the same as in individuals with normal renal function but renal clearance was reduced, resulting in a mean elimination t 1/2 of 41.9 hr (6.2 hr in normal subjects). renal clearance of napa correlated well with clcr. dialysis removed napa from both red blood cells and plasma and increased clt approximately fourfold. dialysis itself resulted in a 77% reduction ...	1999	91468
morphological alterations in the cells of the developing quail oviduct as influenced by estradiol-17 beta and the insecticide kepone: i. observations by light and scanning electron microscopy.		1999	91390
prenatal diagnosis of congenital adrenal hyperplasia.		1999	91004
diagnosis and staging of prostatic cancer by transrectal ultrasonography. a preliminary study.	sixty men (7 normal, 53 with prostatic disease) underwent transrectal ultrasonic scanning of their prostates in order to assess the technique and evaluate its reliability in the detection and staging of prostatic cancer. the prostatic capsule was clearly seen in 58 men; non-integrity of the capsule occurred only in those with proven cancer (17 cases). an ultrasound diagnosis of cancer was made for 32 of 33 men with proven disease and it was shown that ultrasound demonstrated anterior perforation ...	1999	93975
evaluation of four "kit" immunoassay methods for determination of alpha-fetoprotein in serum during pregnancy.	we evaluated four immunoassay methods for determination of alpha-fetoprotein in serum. all are commercially available in kit form in north american and we find them acceptable with respect to sensitivity, stability, precision, linearity, and analytical recovery. for the dainabott method, the median values from 16 through 20 weeks of gestation are 34.4, 38.1, 42.1, 51.4, and 66.3 micrograms/l for each week, respectively. corresponding values for the amersham method are 27.9, 31.4, 34.9, 38.2, and ...	1999	91454
mycosis fungoides with verrucous lesions.		1999	84478
malignant potential of intestinal plasma cells in alpha-chain disease.		1999	84179
[the current discussion: drug treatment of pain caused by cancer].		1999	93853
selenium in the heart of china.		1999	90974
[evaluation of unions of dentists on more solid basis].		1999	96805
treatment of myasthenia gravis. report on 139 patients.	in the treatment of myasthenia gravis (mg) considerable progress has recently been achieved. our experience is based on the observation of 139 patients with an average follow-up of 3 years and 4 months. a treatment plan and results are presented. indications for thymectomy: all cases of mg in adult life, apart from ocular myasthenia without radiological thymoma and without electrophysiological and pharmacological signs of generalization; before puberty only cases with radiological thymoma and se ...	1999	93623
[pituitary reserve of thyrotrophic hormone in hypothyroid children].		1999	103634
current views on immunoglobulin abnormalities and b cell proliferations.		1999	96675
hemolysis induced by cefazolin and cephalothin in a patient with penicillin sensitivity.	a patient with penicillin sensitivity, who had never received a cephalosporin antibiotic previously, developed anemia and spherocytosis following the administration of cefazolin. hemolysis abated when the drug was discontinued on the fourth day, and recurred on day six when cephalothin therapy was begun. igg and complement components were present on the patient's erythrocytes, and igg antibodies in her serum reacted with normal red blood cells which had been coated with benzylpenicillin, cefazol ...	1999	96555
hormonal regulation of osmotic and ionic balance in the haemolymph of the larvae of dragonfly, aeschna cyanea (müller) (odonata : aeschnidae).	the present studies provide an evidence of an active involvement of the neurosecretory a cells of the thoracic ganglia in both, the internal hypo- and hyperosmotic regulation in the larvae of aeschna cyanea.	1999	93445
practical calibration considerations for commissioning an 8mv linear accelerator.		1999	103532
[studies on elastase in pancreatic diseases (author's transl)].		1999	93654
[presence in the sub-esophageal ganglinnof the caterpillar (thaumetopoea pityocampa schiff) of cells containing anti-alpha-endorphins as revealed by immunofluorescence].	immunohistological investigations have been performed with vertebrate neuropeptide antiserums in the subaesophageal ganglion of larval thaumetopoea pityocampa (lepidoptera). positive immunoreaction was observed in two groups of cells only with an alpha-endorphin-antiserum. these cells are azocarmine ground positive earth and paraldehyde-fuchsin ground negative earth or gomori ground negative earth. in the brain, immunohistological staining was completely negative.	1999	97029
[screening in pediatric practice].		1999	96516
[family work: cooperation with families of young schizophrenics--a new dimension in psychiatric nursing].		1999	2326744
nephropathy induced by d-penicillamine.		1999	97051
[pattern and nomenclature of serum proteins of the amphibian urodele ambystoma dumerilii duges].	by means of many electrophoresis techniques, the serum of ambystoma dumerilii was studied. the pattern in cellulose acetate is different from a. mexicanum proteinogram. direct two-dimensional analysis in acetate/polyacrylamid gel with progressively increasing concentration from 4-30%, gives us the opportunity to observe 41 proteic components. by immunological results, the fastest proteic fraction is equivalent to a globuline with alpha-mobility more than an albumin. a nomenclature for this blood ...	1999	96971
cadmium and prostatic carcinoma.		1999	95781
[clinical study of the antiarrhythmic action of mexityl in ventricular disorders of the cardiac rhythm].	the anti-arrhythmic activity of mexitil was studied in 36 patients with frequent, prognostically unfavourable ventricular extrasystoles of various etiology. quantitative and qualitative assessment of rhythm disorders was accomplished by bicycle ergometry and ecg recording for 24 hours by means of portable monitors. the disorders of rhythm were appraised prior to treatment, during mexitil medication in daily doses of 750--1200 mg/24 hrs administered for 3--5 days, and against the background of pl ...	1999	91699
plasma arginine vasopressin in diabetic ketoacidosis.	plasma arginine vasopressin (avp) was measured serially in 10 patients during the first 24 hours of treatment of diabetic ketoacidosis. avp was elevated in all cases initially, ranging from 4.0--122.0 pmol/l, the basal level in healthy hydrated subjects being 1.57 +/- 0.59 pmol/l (+/- 1 sd). the levels fell progressively during the course of treatment. while there was no evidence that the massive increases in avp noted in this study were associated with water overload it remains to be determined ...	1999	103767
[several features of the antioxidant action of chemical carcinogens].	comparative studies of antioxidative effects of phenole inhibitors (vitamine e) amino-and sulfur-containing radioprotectors, and chemical cancerogens on oxidation of unsatured fatty acid were carried out. some polycyclic hydrocarbons and azo-compounds produce a prolonged and stable decrease of peroxide accumulation rate in oxidized oleic acid. due to this property in the in vivo systems chemical cancerogenes can induce prolonged stable changes in the parameters of vitally important redox process ...	1999	96873
[inborn errors of metabolism (author's transl)].		1999	96846
manometry in the evaluation of esophageal function.		1999	99711
prostaglandin e, pessaries for induction of labour.	vaginal pessaries containing 3 mg of prostaglandin e2 were used to induce labour in 200 patients with variable induction features. prostaglandin-induced labour was augmented where necessary by synthetic oxytocin. there was on failed induction. only 23% of patients with favourable induction features and 53% of patients with unfavourable features needed oxytocin. there were no adverse fetal or maternal effects. the prostaglandin e2 pessary was as effective in inducing labour as 350 microgram extra ...	1999	85163
[computerized tomography of intra-articular calcaneus fracture. comparison: radiomorphologic result--clinical result].	in 55 patients with intraarticular fracture of the calcaneus computed tomography of the fractured bone was performed immediately after the accident and 6-12 months later. we recorded the fracture type according to the classification established by essex-lopresti. we evaluated the degree of bony destruction, deformation of the calcaneus and the extent of articular bursting. we recorded the severity of the fracture with reference to a 10-point scale and compared this with the clinical outcome afte ...	1999	1566091
palliative operations for incurable gastric cancer.		1999	93827
[correlation of the hourly and daily clearance in prostate adenoma].		1999	93822
the effects of the evolution of the health care system on the problems of health systems access, cost, and quality.		1999	115955
chido, rodgers and c4. in vivo and in vitro coating of red blood cells, grouping and antibody detection.	c4 sucrose/low ionic strength (lis)-coated red blood cells (rbc) are excellent for the detection of the previously 'nebulous' antibodies, anti-chido and anti-rodgers, as well as for serum/plasma typing of these antigens by an inhibition technique. by enzyme treatment of such cells, it is confirmed that the ch and rg antigens reside on the c4d part of the c4 molecule. freshly taken rbc from normal individuals were examined with a sensitive auto-analyzer technique with anti-chido, anti-c4 and anti ...	1999	91269
significance of the acetylation phenotype and the therapeutic effect of procainamide.	in order to estimate the relative anti-arrhythmic effect of procainamide and n-acetyl-procainamide, 18 randomly selected, patients with arrhythmia were divided into two groups; the first was treated with pronestyl in the first half of the investigation period, followed by duretter in the second half, and the second group began with duretter and terminated with pronestyl. the concentrations of procainamide and n-acetylprocainamide were measured twice a day during the steady state part of each tre ...	1999	84761
[evaluation of blood-liquor barrier by protein gradients in congenital hydrocephalus in infancy].		1999	88669
ectopic pregnancy in southern iran: a statistical review of 96 cases.	a review is presented of 96 cases of ectopic pregnacy over a 7-year period. it is noted that in recent years the incidence of this problem has increased. inadequately diagnosed and treated pelvic inflammatory disease has been found to be the main predisposing factor (40%). the rate of extra-uterine gestation was 1:336 deliveries. in this study, previous fertility was high, and primigravidae constituted only 10.4% of the total. the effect of previous pelvic operations and pelvic infection is disc ...	1999	15916
restricting legal abortion: some maternal and child health effects in romania.	the dramatic rise of the romanian birth rate in 1967 following the restrictive abortion decree of late 1966 is well known. less well known are significant changes in key maternal and child health indicators in the period after 1966. possible reasons for these changes are discussed as are the implications of the romanian experience for population policy and abortion research in the united states.	1999	1115131
critical concerns: national health insurance.		1999	96458
[breathing therapy--ascpects of long-term home treatment of chronic obstructive lung disease (author's transl)].		1999	96434
[gougerot-sjögren syndrome associated with dysimmune adenopathy and mixed cryoglobulinemia].		1999	96425
ontogeny of immunity in amphibians: changes in antibody repertoires and appearance of adult major histocompatibility antigens in xenopus.	larval xenopus anti-2,4-dinitrophenyl antibodies of the low-molecular weight type can be analyzed by isoelectric focusing (ief). within a clone of genetically identical animals, tadpoles make antibodies whose ief spectrotypes are shared by most of the individuals. adults of the same clone also make antibodies of identical spectrotype, but the adult pattern can be very different from the larval one, although both responses are heterogeneous. the larval spectrotypes that one cannot see in a primar ...	1999	93547
vinblastine, methotrexate, bleomycin, in the management of head and neck cancer.		1999	93540
histochemical study of orcein-positive hepatocellular material in paraffin sections of liver biopsy samples in the course of various liver diseases.	a histochemical study was carried out on orcein-positive hepatocellular material in 111 cases, 27 of which were stained positively by orcein. orcein-positive material was very frequently found in protracted viral hepatitis and in chronic active hepatitis, as well as in other liver diseases with or without cholestasis; it was absent in liver cirrhosis. in all the cases considered the orcein-positive material was mainly formed of proteins, rich in amino acids with sulfhydryl and disulphide radical ...	1999	91188
identification tests for acidic and neutral compounds extracted from putrefied viscera.		1999	89185
amino-terminal sequence of the 21 kda apoprotein of a minor light-harvesting pigment-protein complex of the photosystem ii antenna (lhc iid/cp24).	the 21 kda apoprotein of lhc iid, a minor light-harvesting antenna component of photosystem ii, has been isolated and subjected to n-terminal protein sequencing. a sequence of 66 residues was obtained which contains regions of considerable homology to both those reported for lhc ii and lhc i, but which is obviously distinct from them. the proposed occurrence of an identical 21 kda lhc subunit in both photosystems i and ii is shown to be incorrect.	1999	2192916
muscle mass and composition in malnourished infants and children and changes seen after recovery.		1999	96411
[diurnal variations in glucose tolerance in normal subjects and changes induced by oral glibenclamide].		1999	96388
v and c parts of immunoglobulin kappa-chain genes are separate in myeloma.		1999	96352
effect of oral contraceptives on platelet noradrenaline and5-hydroxytryptamine receptors and aggregation.	characteristics of receptor sites on platelets for noradrenaline (na) and serotonin (5-ht) changed significantly between day 21 and day 28 of treatment in healthy women taking oral contraceptives. na-induced and 5-ht-induced platelet aggregation and 5-ht uptake vaired accordingly. in untreated women with normal cycles no changes in either receptor site or in platelet function occurred between the luteal and postmenstrual phases. it is concluded that the observed changes are due to a pharmacologi ...	1999	91026
cryoprecipitate preparation.		1999	84193
ischemic stroke due to protein c deficiency.	plasma protein c exerts anticoagulatory effects by inactivating factors v and viii. hereditary protein c deficiency is transmitted as an autosomal dominant disorder. homozygous individuals usually develop purpura fulminans as newborns; heterozygous protein c-deficient individuals are at increased risk for venous thrombosis and pulmonary embolism. however, arterial thrombosis has been only rarely observed. we describe a young patient with heterozygous protein c deficiency who experienced a severe ...	1999	2195715
[the validation of the use of thymic preparations in tuberculous infection by experimental data].		1999	1866916
sturge-weber syndrome: presentation as a focal seizure disorder without nevus flammeus.		1999	97040
a comparison of pediatric versus adult cadaver donor kidneys for transplantation.		1999	2326916
[surgical treatment of digestive complications of radiotherapy (author's transl)].		1999	96647
crossed cerebellar diaschisis and crossed cerebellar atrophy: correlation of mr findings, clinical symptoms, and supratentorial diseases in 26 patients.	crossed cerebellar diaschisis is a condition in which cerebellar hypometabolism is ascribed to functional disconnection of the contralateral hemisphere from the cerebral cortex. interruption of the cerebropontine-cerebellar pathway is thought to be the most likely mechanism of this remote transneuronal metabolic depression. this phenomenon can be diagnosed by positron emission tomography, as there is hypometabolism in the affected cerebellar hemisphere. the morphologic features of the affected c ...	1999	1566683
[qualitative and quantitative investigations of the area striata in an ontogenic series of male tupaia belangeri].	the cyto-, myelo- and fibrilloarchitectonics of the area striata and its laminae were described in 36 male tupaia belangeri at an age of 36 to 536 days of ontogenesis. the fresh volumes of the area striata and its laminae were determined and the growth of the fresh volumes described with the 6-parametric growth-function: (formula: see text) likewise, the matter lying directly under the area striata were determined and these data were analysed with the logistic growth-function: (formula: see text ...	1999	408411
severe hypertension in primary aldosteronism and good response to surgery.	11 patients with primary aldosteronism have been encountered over 11 years and submitted to surgery in a provincial teaching hospital serving a population of 3 million. contrary to classical teaching, the hypertension has usually been very severe. precise identification of the site of the lesion preoperatively has been possible by the measurement of adrenal-vein aldosterone levels, and results of surgery have been excellent. the iodocholesterol adrenal scan also correctly identified the site of ...	1999	85065
the medi-drama as an instrument to teach doctor-patient relationships.	this paper reviews an experience of the curriculum development group of the college of family physicians of canada in describing the doctor-patient relationship and its value in the clinical process. it proposes the use of a medi-drama or multi-scene script encompassing a broad range of bio-psycho-social-ethical issues as a practical tool to teach the doctor-patient relationship. principles for conducting a medi-drama are presented, as are the advantages of this experiential teaching modality. a ...	1999	2640693
replacement therapy in the mucopolysaccharidoses.		1999	103935
comparison between hla-d (lcl) and dr typing.		1999	93807
sodium iron nafeedta as an iron fortification compound in central america. absorption studies.	studies were performed in seven children and 98 adults to compare the proportion of iron absorbed when administered as ferric sulfate (fe2(so4)3), nafeedta, hemoglobin (hb), and ferrous ascorbate. studies in children (mostly iron deficient) showed that when the compounds were given with a milk-rice-sugar formula totalling 5 mg fe, iron from hemoglobin was absorbed best, followed by nafeedta and by fe2(so4)3 (mean percent absorption +/-sd = 34.5 +/- 1.5, 8.6 +/- 1.9 and 3.3 +/- 1.5, respectively) ...	1999	96688
[comparative studies on the demonstrability of eosinophilic granuloma using biebrich's scarlet staining and adam's reaction].		1999	85391
hyperosmolar hyperglycemic non-ketotic syndrome treated without insulin: case reports.		1999	99698
[smoking and serum alpha 1-antitrypsin in 1296 healthy men (author's transl)].	serum alpha 1-antitrypsin (alpha 1at) was measured by radial immunodiffusion in 1296 healthy men aged 18--50 years. other biological criteria, including leukocyte count and alpha 2-globulins were measured and the subjects were given a detailed questionnaire on their smoking habits. results showed a very strong positive relationship between smoking and serum alpha 1at: the heavy smokers had a serum alpha 1at 20% higher than the non-smokers, and among subjects who stopped smoking, the level return ...	1999	93523
[value and limit of photocoagulation for juxtafoveolar sub-retinal vessels (author's transl)].	sub-retinal new vessels arising from the choroid are the most dangerous components in disciform maculopathies. the greatest advance made in their treatment has been due to the use of photocoagulation over the last few years, but their destruction by this method is still a very controversial subject. the aim of the present study was to define which lesions should be treated and which should not. results demonstrated that the use of photocoagulation to destroy new vascular membrane which is very c ...	1999	94069
some response characteristics of co2 electrodes.	radiometer carbon dioxide electrodes and probably other electrodes of similar design do not give the same readings, except under certain very limited conditions, when used to measure carbon dioxide concentrations in solution and the gas phase with which the liquid is in equilibrium. blood samples of high p(co2), when introduced into the sample chamber of an electrode at low p(co2), lose co2 to the electrode resulting in a reduction of sample p(co2) and hence, because the sample size is very smal ...	1999	96504
[high sexual selectivity of hydrazine derivatives potentiating the antitumor effect of cytostatics].		1999	103698
molecular perspectives of monovalent cation selective transmembrane channels.		1999	94042
prenatal methylmercury poisoning. clinical observations over five years.	thirty-two infants prenatally exposed to methylmercury and their mothers were examined over a five-year period after the iraqi methylmercury epidemic. severity of poisoning in mothers was related to the peak mercury concentration in their hair and in the infants to the maximum concentration in maternal hair during pregnancy. in nine cases of cerebral palsy, methylmercury exposure occurred only during the last trimester. all infants except three (two were orphaned soon after birth and one was bot ...	1999	84530
screening for pancreatic disease: a comparison of grey-scale ultrasonography and isotope scanning.	the efficiency of ultrasound in the diagnosis of pancreatic disease was compared prospectively with that of selenomethionine isotope scanning in 46 patients presenting with abdominal pain or weight-loss or with jaundice. of 14 patients who later proved to have pancreatic carcinoma, all had an abnormal isotope scan and 13 had an abnormal ultrasound scan. of 10 patients with chronic pancreatitis, all had an abnormal isotope scan and 9 had an abnormal ultrasound scan. the small advantage of selenom ...	1999	85871
cure of burkitt's lymphoma. ten-year follow-up of 157 ugandan patients.	192 ugandan patients with burkitt's lymphoma were treated with various regimens containing high-dose cyclophosphamide. 21 patients died during induction therapy, and 14 died after partial responses. of 157 patients who responded completely to treatment, 16 were lost to follow-up (10 in 69 died and 72 (51%) are alive and disease-free. 31 of the long-term survivors have relapsed at least once and have been successfully retreated. overall, 34 surviving patients had central-nervous-system involvemen ...	1999	91027
two effects of phosphodiesterase inhibitors on limulus ventral photoreceptors.		1999	91407
[treatment of allergic non-seasonal rhinitis with intal].		1999	91152
cellular changes in the toad urinary bladder in response to metabolic acidosis.	the urinary bladder of bufo marinus excretes h+ and nh+4, and the h+ excretion is increased when the animal is placed in metabolic acidosis. the mitochondria-rich (mr) cells mediate the h+ excretion by the bladder. the purpose of this study was to determine if there is a change in mr cells of the bladder during metabolic acidosis. bladders from normal toads and from toads that had been placed in metabolic acidosis were used. the bladders were mounted between plastic chambers and h+ excretion mea ...	1999	96269
antimicrobial effects of psidium guajava extract as one mechanism of its antidiarrhoeal action.	a morphine-like spasmolytic action (not naloxone reversible; involving the inhibition of acetylcholine release) and also effects on the transmural transport of electrolytes (na(+) and k(+)) and water have been reported as possible modes of the antidiarrhoeal action of polar fractions of psidium guajava leaf extractives. the objective for this study was to verify if the reported modes of the antidiarrhoeal action should be broadened to include direct antimicrobial actions on some of the more comm ...	1999	22589684
antibacterial activity of a chitooligosaccharide mixture prepared by cellulase digestion of shrimp chitosan and its application to milk preservation.	the antibacterial activity of a chitooligosaccharide mixture prepared by digestion of shrimp chitosan with cellulase at 50 degrees c for 14 h was evaluated. sugars with 1 to 8 degrees of polymer (dp) were found in this chitooligosaccharide mixture, and the weight percentage of sugars with dp > or = 6 was 44.3%. minimal lethal concentrations of this mixture against aeromonas hydrophila, escherichia coli, listeria monocytogenes, pseudomonas aeruginosa, salmonella typhimurium, shigella dysenteriae, ...	2000	10852568
vibrio vulnificus has the transmembrane transcription activator toxrs stimulating the expression of the hemolysin gene vvha.	in an attempt to dissect the virulence regulatory mechanism in vibrio vulnificus, we tried to identify the v. cholerae transmembrane virulence regulator toxrs (toxrs(vc)) homologs in v. vulnificus. by comparing the sequences of toxrs of v. cholerae and v. parahaemolyticus (toxrs(vp)), we designed a degenerate primer set targeting well-conserved sequences. using the pcr product as an authentic probe for southern blot hybridization, a 1.6-kb bglii-hindiii fragment and a 1.2-kb hindiii fragment con ...	2000	10852871
functional analysis of genes in the rfb locus of leptospira borgpetersenii serovar hardjo subtype hardjobovis.	lipopolysaccharide (lps) is a key antigen in immunity to leptospirosis. its biosynthesis requires enzymes for the biosynthesis and polymerization of nucleotide sugars and the transport through and attachment to the bacterial membrane. the genes encoding these functions are commonly clustered into loci; for leptospira borgpetersenii serovar hardjo subtype hardjobovis, this locus, named rfb, spans 36.7 kb and contains 31 open reading frames, of which 28 have been assigned putative functions on the ...	2000	10858186
use of rna arbitrarily primed-pcr fingerprinting to identify vibrio cholerae genes differentially expressed in the host following infection.	evidence suggests that a repertoire of vibrio cholerae genes are differentially expressed in vivo, and regulation of virulence factors in vivo may follow a different pathway. our work was aimed at characterization of in vivo-grown bacteria and identification of genes that are differentially expressed following infection by rna arbitrarily primed (rap)-pcr fingerprinting. the ligated rabbit ileal loop model was used. the motility of in vivo-grown bacteria increased by 350% over that of in vitro-g ...	2000	10858198
detection of dna via an ion channel switch biosensor.	detection of dna by an ion channel switch biosensor has been demonstrated in a model system, using single-stranded oligonucleotide sequences of 52-84 bases in length. two different biotinylated probes are bound, via streptavidin, either to the outer region of a gramicidin ion channel dimer or to an immobilized membrane component. the ion channels are switched off upon detection of dna containing complementary epitopes to these probes, separated by a nonbinding region, at nanomolar levels. the dn ...	2000	10860501
bacterial, viral and parasitic enteric pathogens associated with acute diarrhea in hospitalized children from northern jordan.	to determine the etiology of acute diarrhea in jordanian children under 5 years of age, we examined stool samples from 265 children admitted to the pediatric ward at princess rahma hospital for children, irbid, jordan, for parasites, rotavirus and enteric bacteria. using both traditional and molecular diagnostic techniques, we detected enteropathogens in 66.4% of patients with diarrhea. a single enteric pathogen was detected in 50.9% of the children, and multiple pathogens were detected in 15.5% ...	2000	10865179
cholera incidence and el niño-related higher ambient temperature.		2000	10865299
two-year study of the protective efficacy of the oral whole cell plus recombinant b subunit cholera vaccine in peru.	the protective efficacy of an oral inactivated whole cell vibrio cholerae plus recombinant b subunit cholera vaccine was determined against el tor cholera among peruvian children and adults (2-65 years old) in a randomized, double-blind manner. study subjects received 2 doses of vaccine or placebo 2 weeks apart, followed by a booster dose 10 months later. surveillance for cholera was performed actively, with 2 visits per week to each household, and passively, at a local hospital. stool samples w ...	2000	10823767
local and systemic immune responses to combined vibrio cholerae cvd103-hgr and salmonella typhi ty21a live oral vaccines after primary immunization and reimmunization.	the local and systemic antibody responses elicited following concomitant primary immunization and reimmunization with the live oral attenuated vibrio cholerae cvd103-hgr and salmonella typhi ty21a vaccine strains were determined in healthy adult volunteers. a more pronounced serum vibriocidal antibody response was generated after primary immunization compared to reimmunization 2.5 or 3.5 yr later. the seroconversion rate (> or =4-fold rise over baseline) was 81% subsequent to primary immunizatio ...	2000	10825607
health impairments arising from drinking water resources contaminated with vibrio cholerae.	the endemic and seasonal nature of cholera depends upon the survival of toxigenic vibrio cholerae in various niches of the aquatic environment. to understand the transmission and ecology of v. cholerae, it is necessary to know which component in the aquatic ecosystem can harbor it and thus contribute to the endemic presence. toxigenic v. cholerae is now recognized as an autochthonous member of the microflora in many aquatic environments based on its protracted survival and proliferation without ...	2000	10842790
identification of a novel gene, fimv, involved in twitching motility in pseudomonas aeruginosa.	transposon mutagenesis was used to identify a new locus required for twitching motility in pseudomonas aeruginosa. four tn5-b21 mutants which lacked twitching motility and a fifth which exhibited impaired motility were found to map to the same kpn:i restriction fragment at approximately 40 min on the p. aeruginosa genome. cloning and sequencing studies showed that all five transposon insertions occurred within the same 2.8 kb orf, which was termed fimv. the product of this gene has a putative pe ...	2000	10846211
a ganglioside-based assay for cholera toxin using an array biosensor.	a rapid assay for cholera toxin (ct) has been developed using a fluorescence-based biosensor. this sensor was capable of analyzing six samples simultaneously for ct in 20 min with few manipulations required by the operator. the biochemical assays utilized a ganglioside-"capture" format: ganglioside gm1, utilized for capture of analyte, was immobilized in discrete locations on the surface of the optical waveguide. binding of ct to immobilized gm1 was demonstrated with direct assays (using fluores ...	2000	10847619
evidence for a signaling system in helicobacter pylori: detection of a luxs-encoded autoinducer.	helicobacter pylori possesses a homolog of the luxs gene, initially identified by its role in autoinducer production for the quorum-sensing system 2 in vibrio harveyi. the genomes of several other species of bacteria, notably escherichia coli, salmonella enterica serovar typhimurium, and vibrio cholerae, also include luxs homologs. all of these bacteria have been shown to produce active autoinducers capable of stimulating the expression of the luciferase operon in v. harveyi. in this report, we ...	2000	10850976
ecological study of vibrio cholerae in vellore.	vellore is endemic for cholera due to vibrio cholerae o1 and o139. in a previous study the prevalence of vibrio cholerae in drinking water, lakes and sewage outfalls in a single 2-months period in vellore, india was determined. in addition water samples from three sites were also tested for the presence of v. cholerae o1 and o139 by fluorescent antibody staining. this follow on study has examined how the environmental distribution of v. cholerae at the same sites alters over a 12-month period an ...	2000	10813143
antibiotic resistance in the ecor collection: integrons and identification of a novel aad gene.	the 72 escherichia coli strains of the ecor collection were examined for resistance to 10 different antimicrobial agents including ampicillin, tetracycline, mercury, trimethoprim, and sulfonamides. eighteen strains were resistant to at least one of the antibiotics tested, and nearly 20% (14 of 72) were resistant to two or more. several of the resistance determinants were shown to be carried on conjugative elements. the collection was screened for the presence of the three classes of integrons an ...	2000	10817710
susceptibility of vibrio cholerae o139 to antibody-dependent, complement-mediated bacteriolysis.	volunteer studies with vibrio cholerae o1 have shown that the best correlate of a vaccine's protective efficacy is its propensity to elicit serum bactericidal responses in its recipients. attempts to detect such responses following infection with v. cholerae o139, however, have met with varying success. using a tube-based assay which involves viable counting, we now report that strains of serogroup o139 can appear to be sensitive or resistant to a fixed concentration of complement in the presenc ...	2000	10799459
zonulin, a newly discovered modulator of intestinal permeability, and its expression in coeliac disease.	we identified zonulin, a novel human protein analogue to the vibrio cholerae derived zonula occludens toxin, which induces tight junction disassembly and a subsequent increase in intestinal permeability in non-human primate intestinal epithelia. zonulin expression was raised in intestinal tissues during the acute phase of coeliac disease, a clinical condition in which tight junctions are opened and permeability is increased.	2000	10801176
purification and characterization of n-acetylglucosamine-6-phosphate deacetylase from a psychrotrophic marine bacterium, alteromonas species.	a psychrotrophic bacterium, strain mct-9, which produced an n-acetylglucosamine-6-phosphate deacetylase, was isolated from a deep-seawater sample in the mariana trough. the mct-9 strain was identified as alteromonas sp. the native enzyme had a molecular mass of 164,000 da, and was predicted to be composed of four identical subunits with molecular masses of 41,000 da. the purified enzyme hydrolyzed n-acetylglucosamine (glcnac), glcnac-6-phosphate, and glcnac-6-sulfate. considering the low k(m) an ...	2000	10804240
the marine pathogen vibrio vulnificus encodes a putative homologue of the vibrio harveyi regulatory gene, luxr: a genetic and phylogenetic comparison.	vibrio vulnificus is an opportunistic pathogen that exhibits numerous virulence factors, including the secretion of a zinc metalloprotease and the production of a capsule. we have cloned and sequenced a gene from v. vulnificus that is a homologue of the positive transcriptional regulator, luxr, of the lux operon in vibrio harveyi. this gene encodes a putative, single complete open reading frame designated smcr, which shares greater than 75% nucleotide identity with luxr of v. harveyi. the deduce ...	2000	10806366
[the cultivation of vibrio cholerae with green algae in an experiment].	relationships between vibrio cholerae of different origin and some serogroups with green algae scenedesmus quadricauda in mineral medium at two temperatures have been experimentally studied. differences in the relationships of various strains with green algae under the above-mentioned experimental conditions have been established. the study has shown that a decrease in the concentration and the death of vct+ and vct- vibrios of all strains under study occur in the linear phase of the development ...	2000	10808566