Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| degradation and conversion of endosulfan by newly isolated pseudomonas mendocina zam1 strain. | endosulfan contamination is one of the major concerns of soil ecosystem, which causes detrimental effects not only to humans but also to animals and plants. therefore, the aim of this study was to isolate and identify a novel bacterial strain capable of degrading endosulfan in agriculture contaminated soils. a novel bacterial strain was isolated from the sugarcane field contaminated with endosulfan, and was named as zam1 strain. the zam1 bacterial strain was further identified as pseudomonas men ... | 2017 | 28667651 |
| exacerbation of bronchiectasis by pseudomonas monteilii: a case report. | pseudomonas spp are important opportunistic and nosocomial pathogens. one such species is pseudomonas monteilii (p. monteilii). it has been described as an environmental contaminant and potential pathogen. we identified this organism as the causative agent of an exacerbation of bronchiectasis and an environmental contaminant in our hospital on two separate occasions. | 2017 | 28738817 |
| effects of humic acid on the interactions between zinc oxide nanoparticles and bacterial biofilms. | the effects of humic acid (ha) on interactions between zno nanoparticles (zno nps) and pseudomonas putida kt2440 biofilms at different maturity stages were investigated. three stages of biofilm development were identified according to bacterial adenosine triphosphate (atp) activity associated with biofilm development process. in the initial biofilm stage 1, the atp content of bacteria was reduced by more than 90% when biofilms were exposed to zno nps. however, in the mature biofilm stages 2 and ... | 2017 | 28851497 |
| growth phase-dependent expression profiles of three vital h-ns family proteins encoded on the chromosome of pseudomonas putida kt2440 and on the pcar1 plasmid. | h-ns family proteins are nucleoid-associated proteins that form oligomers on dna and function as global regulators. they are found in both bacterial chromosomes and plasmids, and were suggested to be candidate effectors of the interaction between them. tura and turb are the predominantly expressed h-ns family proteins encoded on the chromosome of pseudomonas putida kt2440, while pmr is encoded on the carbazole-degradative incompatibility group p-7 plasmid pcar1. previous transcriptome analyses s ... | 2017 | 28851281 |
| a bacterial multi-domain nad-independent d-lactate dehydrogenase utilizes fad and fe-s clusters as cofactors and quinone as electron acceptor for d-lactate oxidization. | bacterial membrane-associated nad-independent d-lactate dehydrogenase (fe-s d-ildh) oxidizes d-lactate into pyruvate. sequence analysis of the enzyme reveals that it contains a fe-s oxidoreductase domain in addition to an fad-containing dehydrogenase domain, which differs from other typical d-ildhs. fe-s d-ildh from pseudomonas putida kt2440 was purified as a his-tagged protein and characterized in detail. this monomeric enzyme exhibited activities with l-lactate and several d-2-hydroxyacids. qu ... | 2017 | 28847921 |
| electrochemical hydroxylation of c3-c12 n-alkanes by recombinant alkane hydroxylase (alkb) and rubredoxin-2 (alkg) from pseudomonas putida gpo1. | an unprecedented method for the efficient conversion of c3-c12 linear alkanes to their corresponding primary alcohols mediated by the membrane-bound alkane hydroxylase (alkb) from pseudomonas putida gpo1 is demonstrated. the x-ray absorption spectroscopy (xas) studies support that electrons can be transferred from the reduced alkg (rubredoxin-2, the redox partner of alkb) to alkb in a two-phase manner. based on this observation, an approach for the electrocatalytic conversion from alkanes to alc ... | 2017 | 28827709 |
| heterologous expression of phac(2) gene and poly-3-hydroxyalkanoate production by recombinant cupriavidus necator strains using canola oil as carbon source. | many heterologous transformation studies have been carried out using the cupriavidus necator phb(-4) strain to investigate the expression characteristics of various polyhydroxyalkanoate (pha) synthase enzymes. in this study, we generated a recombinant c. necator phb(-4) strain by transforming a plasmid (pmrc03) harbouring the synthetic phac2 gene of pseudomonas putida ca-3. under conditions favourable for expression of the phac2 p.putca-3 gene, canola oil was used as carbon source for the synthe ... | 2017 | 28827158 |
| crystal structures reveal metal-binding plasticity at the metallo-β-lactamase active site of pqqb from pseudomonas putida. | pqqb is an enzyme involved in the biosynthesis of pyrroloquinoline quinone and a distal member of the metallo-β-lactamase (mbl) superfamily. pqqb lacks two residues in the conserved signature motif hxhxdh that makes up the key metal-chelating elements that can bind up to two metal ions at the active site of mbls and other members of its superfamily. here, we report crystal structures of pqqb bound to mn(2+), mg(2+), cu(2+), and zn(2+). these structures demonstrate that pqqb can still bind metal ... | 2017 | 28825148 |
| oil biodegradation: interactions of artificial marine snow, clay particles, oil and corexit. | during the deepwater horizon (dwh) oil spill, interactions between oil, clay particles and marine snow lead to the formation of aggregates. interactions between these components play an important, but yet not well understood, role in biodegradation of oil in the ocean water. the aim of this study is to explore the effect of these interactions on biodegradation of oil in the water. laboratory experiments were performed, analyzing respiration and n-alkane and btex biodegradation in multiple condit ... | 2017 | 28821355 |
| pseudomonas wadenswilerensis sp. nov. and pseudomonas reidholzensis sp. nov., two novel species within the pseudomonas putida group isolated from forest soil. | within the frame of a biotechnological screening, we isolated two pseudomonas strains from forest soil. 16s rrna gene sequence analysis indicated that strain ccos 864t shared 99.8 % similarity with pseudomonas donghuensis hyst, while strain ccos 865t shared 99.0 % similarity with pseudomonas putida dsm 291t and lower similarity with other p. putida group type strains. based on multilocus sequence analysis, the two strains were genotypically distinct from each other, each forming a separate clade ... | 2017 | 28820109 |
| photoautotrophic production of polyhydroxyalkanoates in a synthetic mixed culture of synechococcus elongatus cscb and pseudomonas putida cscab. | one of the major challenges for the present and future generations is to find suitable substitutes for the fossil resources we rely on today. cyanobacterial carbohydrates have been discussed as an emerging renewable feedstock in industrial biotechnology for the production of fuels and chemicals, showing promising production rates when compared to crop-based feedstock. however, intrinsic capacities of cyanobacteria to produce biotechnological compounds are limited and yields are low. | 2017 | 28814973 |
| oxygen mass transfer and shear stress effects on pseudomonas putida bcrc 14365 growth to improve bioreactor design and performance. | in this work, the experimental evidence is presented for two basic issues including oxygen mass transfer and shear analysis on the microorganism containing medium on the most prominent sections of the bioreactor. computational fluid dynamics (cfd) methodology reproduces shear rate values for specific impeller designs using the commercial code (fluent 6.2). cfd calculates volumetric mass transfer coefficient based on the higbie's penetration theory. four types of impeller are used. the spherical ... | 2017 | 28803423 |
| identification and elucidation of in vivo function of two alanine racemases from pseudomonas putida kt2440. | the genome of pseudomonas putida kt2440 contains two open reading frames (orfs), pp_3722 and pp_5269, that encode proteins with a pyridoxal phosphate (plp) binding motif and a high similarity to alanine racemases. alanine racemases play a key role in the biosynthesis of d-alanine, a crucial amino acid in the peptidoglycan layer. for these orfs, we generated single and double mutants and found that inactivation of pp_5269 resulted in d-alanine auxotrophy, while inactivation of pp_3722 did not. fu ... | 2017 | 28799718 |
| inactivation combined with cell lysis of pseudomonas putida using a low pressure carbon dioxide microbubble technology. | inactivation processes can be classified into non-thermal inactivation methods such as ethylene oxide and γ-radiation, and thermal methods such as autoclaving. the ability of carbon dioxide enriched microbubbles to inactivate pseudomonas putida suspended in physiological saline, as a non-thermal sterilisation method, was investigated in this study with many operational advantages over both traditional thermal and non-thermal sterilisation methods. | 2017 | 28781404 |
| production, biophysical characterization and crystallization of pseudomonas putida graa and its complexes with grat and the grata operator. | the grata operon from pseudomonas putida encodes a toxin-antitoxin module with an unusually moderate toxin. here, the production, saxs analysis and crystallization of the antitoxin graa, the grata complex and the complex of graa with a 33 bp operator fragment are reported. graa forms a homodimer in solution and crystallizes in space group p21, with unit-cell parameters a = 66.9, b = 48.9, c = 62.7 å, β = 92.6°. the crystals are likely to contain two graa dimers in the asymmetric unit and diffrac ... | 2017 | 28777088 |
| chemoenzymatic synthesis of a novel borneol-based polyester. | terpenes are a class of natural compounds that have recently moved into the focus as a bio-based resource for chemical production, owing to their abundance, their mostly cyclic structures, and the presence of olefin or single hydroxy groups. to apply this raw material in new industrial fields, a second hydroxy group is inserted into borneol by cytochrome p450cam (cyp101) enzymes in a whole-cell catalytic biotransformation with pseudomonas putida kt2440. next, a semi-continuous batch system was d ... | 2017 | 28772002 |
| mass cytometry for detection of silver at the bacterial single cell level. | background: mass cytometry (cytometry by time of flight, cytof) allows single-cell characterization on the basis of specific metal-based cell markers. in addition, other metals in the mass range such as silver can be detected per cell. bacteria are known to be sensible to silver and a protocol was developed to measure both the number of affected cells per population and the quantities of silver per cell. methods: for mass cytometry ruthenium red was used as a marker for all cells of a population ... | 2017 | 28769897 |
| a novel strategy to immobilize bacteria on polymer particles for efficient adsorption and biodegradation of soluble organics. | a novel strategy was used to immobilize bacterial cells on the surface of functional polymer particles for the efficient adsorption and biodegradation of organics in wastewater. first, the bacterial cells were aggregated using a vinyl-containing pre-polymer, and the obtained bacteria-pre-polymer complex was then used as a particle stabilizer to construct a stable pickering emulsion of functional cross-linking monomers and hydrophobic superparamagnetic iron oxide nanoparticles (the oil phase) in ... | 2017 | 28767111 |
| evaluation of bioaugmentation and biostimulation on arsenic remediation in soil through biovolatilization. | arsenic (as) removal through microbially driven biovolatilization can be explored as a potential method for as bioremediation. however, its effectiveness needs to be improved. biostimulation with organic matter amendment and bioaugmentation with the inoculation of genetic engineered bacteria could be potential strategies for as removal and site remediation. here, the experiments were conducted to evaluate the impacts of rice straw and biochar amendment, inoculation of genetic engineered pseudomo ... | 2017 | 28766144 |
| combinatorial metabolic engineering of pseudomonas putida kt2440 for efficient mineralization of 1,2,3-trichloropropane. | an industrial waste, 1,2,3-trichloropropane (tcp), is toxic and extremely recalcitrant to biodegradation. to date, no natural tcp degraders able to mineralize tcp aerobically have been isolated. in this work, we engineered a biosafety pseudomonas putida strain kt2440 for aerobic mineralization of tcp by implantation of a synthetic biodegradation pathway into the chromosome and further improved tcp mineralization using combinatorial engineering strategies. initially, a synthetic pathway composed ... | 2017 | 28765600 |
| municipal wastewater treatment via co-immobilized microalgal-bacterial symbiosis: microorganism growth and nutrients removal. | a symbiotic microalgal-bacterial system may be an optional technology for wastewater treatment. in this study, co-immobilized of a bacterium isolated from a municipal wastewater treatment plant (pseudomonas putida) and a microalgae chlorella vulgaris was used in the study of cell growth and nutrient removal during wastewater treatment under batch and continuous culture conditions. under batch culture conditions, co-immobilization treatment significantly increased the cell density of c. vulgaris ... | 2017 | 28738545 |
| surface complexation modeling of cu(ii) sorption to montmorillonite-bacteria composites. | surface complexation modeling, isothermal titration calorimetry, and batch adsorption were employed to characterize the adsorption of cu onto montmorillonite, pseudomonas putida x4, and their composites at mass ratios of 2:1, 6:1, and 12:1, respectively. different enthalpy values were found for cu adsorption to permanent (-6.43kj/mol) and variable charge sites (8.51kj/mol) on montmorillonite. the component additivity (ca) method was used to predict the adsorption of cu on the composites by combi ... | 2017 | 28738531 |
| gene expression during btex biodegradation by a microbial consortium acclimatized to unleaded gasoline and a pseudomonas putida strain (hm346961) isolated from it. | pseudomonas putida strain (hm346961) was isolated from a consortium of bacteria acclimatized to unleaded gasoline-contaminated water. the consortium can efficiently remove benzene, toluene, ethylbenzene and xylene (btex) isomers, and a similar capability was observed with the p. putida strain. proteome of this strain showed certain similarities with that of other strains exposed to the hydrocarbon compounds. furthermore, the toluene di-oxygenase (tod) gene was up-regulated in p. putida strain wh ... | 2017 | 28735314 |
| transcriptional modulation of transport and metabolism associated gene clusters leading to utilization of benzoate in preference to glucose in pseudomonas putida csv86. | the effective elimination of xenobiotic pollutants from the environment can be achieved by efficient degradation by microorganisms even in the presence of sugars or organic acids. pseudomonas putida csv86 displays a unique property to utilize aromatic compounds prior to glucose. the draft genome and transcription analyses revealed that glucose uptake and benzoate transport and metabolism genes are clustered at glc and ben loci, respectively, as two distinct operons. when grown on glucose+benzoat ... | 2017 | 28733285 |
| foam adsorption as an ex situ capture step for surfactants produced by fermentation. | in this report, a method for a simultaneous production and separation of a microbially synthesized rhamnolipid biosurfactant is presented. during the aerobic cultivation of flagella-free pseudomonas putida em383 in a 3.1l stirred tank reactor on glucose as a sole carbon source, rhamnolipids are produced and excreted into the fermentation liquid. here, a strategy for biosurfactant capture from rhamnolipid enriched fermentation foam using hydrophobic-hydrophobic interaction was investigated. five ... | 2017 | 28723386 |
| delignification and enhanced gas release from soil containing lignocellulose by treatment with bacterial lignin degraders. | the aim of the study was to isolate bacterial lignin-degrading bacteria from municipal solid waste soil, and to investigate whether they could be used to delignify lignocellulose-containing soil, and enhance methane release. | 2017 | 28393443 |
| controlling the regioselectivity of baeyer-villiger monooxygenases by mutation of active-site residues. | baeyer-villiger monooxygenase (bvmo)-mediated regiodivergent conversions of asymmetric ketones can lead to the formation of "normal" or "abnormal" lactones. in a previous study, we were able to change the regioselectivity of a bvmo by mutation of the active-site residues to smaller amino acids, which thus created more space. in this study, we demonstrate that this method can also be used for other bvmo/substrate combinations. we investigated the regioselectivity of 2-oxo-δ(3) -4,5,5-trimethylcyc ... | 2017 | 28504873 |
| suppression of enteric bacteria by bacteriophages: importance of phage polyvalence in the presence of soil bacteria. | bacteriophages are widely recognized for their importance in microbial ecology and bacterial control. however, little is known about how phage polyvalence (i.e., broad host range) affects bacterial suppression and interspecies competition in environments harboring enteric pathogens and soil bacteria. here we compare the efficacy of polyvalent phage pef1 versus coliphage t4 in suppressing a model enteric bacterium (e. coli k-12) in mixtures with soil bacteria (pseudomonas putida f1 and bacillus s ... | 2017 | 28414441 |
| structural characterization of pyoverdines produced by pseudomonas putida kt2440 and pseudomonas taiwanensis vlb120. | the previously unknown sequences of several pyoverdines (pvd) produced by a biotechnologically-relevant bacterium, namely, pseudomonas taiwanensis vlb120, were characterized by high performance liquid chromatography (hplc)-high resolution mass spectrometry (hrms). the same structural characterization scheme was checked before by analysis of pseudomonas sp. putida kt2440 samples with known pvds. a new sample preparation strategy based on solid-phase extraction was developed, requiring significant ... | 2017 | 28631237 |
| presence of vim-positive pseudomonas species in chickens and their surrounding environment. | metallo-β-lactamase gene blavim was identified on the chromosome of four pseudomonas sp. isolates from a chicken farm, including one pseudomonas aeruginosa isolate from a swallow (yanornis martini), one pseudomonas putida isolate from a fly, and two p. putida isolates from chickens. the four isolates shared two variants of blavim-carrying genomic contexts that resemble the corresponding regions of clinical metallo-β-lactamase-producing pseudomonas spp. our study suggests that the surveillance of ... | 2017 | 28438943 |
| draft genome sequence of acinetobacter johnsonii c6, an environmental isolate engaging in interspecific metabolic interactions. | acinetobacter johnsonii c6 originates from creosote-polluted groundwater and performs ecological and evolutionary interactions with pseudomonas putida in biofilms. the draft genome of a. johnsonii c6 is 3.7 mbp and was shaped by mobile genetic elements. it reveals genes facilitating the biodegradation of aromatic hydrocarbons and resistance to antimicrobials and metals. | 2017 | 28428290 |
| in vitro selection and identification of potential probiotics isolated from the gastrointestinal tract of nile tilapia, oreochromis niloticus. | fish gut bacteria can be used as probiotics for aquaculture. the aim of this study is to screen and identify beneficial probiotic bacteria from the gut of nile tilapia, oreochromis niloticus. nine out of one hundred thirty-five isolates were non-pathogenic through intraperitoneal injection and had antibacterial activities with at least a strain from the five isolated fish pathogens, aeromonas sobria, aeromonas hydrophila, pseudomonas aeruginosa, pseudomonas putida, and staphylococcus aureus. fur ... | 2017 | 28819794 |
| evaluation of nutrients removal (no3-n, nh3-n and po4-p) with chlorella vulgaris, pseudomonas putida, bacillus cereus and a consortium of these microorganisms in the treatment of wastewater effluents. | in this research removal of nh3-n, no3-n and po4-p nutrients from municipal wastewater was studied, using chlorella vulgaris, pseudomonas putida, bacillus cereus and an artificial consortium of them. the objective is to analyze the performance of these microorganisms and their consortium, which has not been previously studied for nutrient removal in municipal wastewater. a model wastewater was prepared simulating the physicochemical characteristics found at the wastewater plant in chapala, mexic ... | 2017 | 28708609 |
| molecular investigation on the binding of cd(ii) by the binary mixtures of montmorillonite with two bacterial species. | bacteria-phyllosilicate complexes are commonly found in natural environments and are capable of immobilizing trace metals. however, the molecular binding mechanisms of heavy metals to these complex aggregates still remain poorly understood. this study investigated cd adsorption on gram-positive bacillus subtilis, gram-negative pseudomonas putida and their binary mixtures with montmorillonite using surface complexation model, cd k-edge extended x-ray absorption fine structure (exafs) spectroscopy ... | 2017 | 28754562 |
| dual infection by burkholderia cepaciaand pseudomonas putida in an infective endocarditis case. | infective endocarditis is rarely caused by burkholderia cepacia. pseudomonas putidahas not been reported to cause infective endocarditis so far. this is the first case of infective endocarditis being reported, that is caused by pseudomonas putidaand burkholderia cepaciain an immunocompetent host with no predisposing factors. aortic valve replacement surgery was carried out and antibiotics were given, to which the patient responded well and recovered. | 2017 | 28689527 |
| igm-mediated autoimmune responses to oxidative specific epitopes, but not nitrosylated adducts, are significantly decreased in pregnancy: association with bacterial translocation, perinatal and lifetime major depression and the tryptophan catabolite (trycat) pathway. | immunoglubulin (ig)m responses directed to oxidative specific epitopes (oses) and nitric oxide (no)-adducts are significantly associated with major depression and physio-somatic symptoms. end of term serum igm responses to oses and no-adducts were assayed in pregnant women with (n = 24) and without prenatal depression (n = 25) as well as in 24 non-pregnant women. associations of igm/iga responses to gram-negative gut commensal bacteria (leaky gut index) and iga/igm responses to tryptophan catabo ... | 2017 | 28600633 |
| a versatile one-step crispr-cas9 based approach to plasmid-curing. | plasmids are widely used and essential tools in molecular biology. however, plasmids often impose a metabolic burden and are only temporarily useful for genetic engineering, bio-sensing and characterization purposes. while numerous techniques for genetic manipulation exist, a universal tool enabling rapid removal of plasmids from bacterial cells is lacking. | 2017 | 28764701 |
| construction and optimization of a heterologous pathway for protocatechuate catabolism in escherichia coli enables bioconversion of model aromatic compounds. | the production of biofuels from lignocellulose yields a substantial lignin byproduct stream that currently has few applications. biological conversion of lignin-derived compounds into chemicals and fuels has the potential to improve the economics of lignocellulose-derived biofuels, but few microbes are able both to catabolize lignin-derived aromatic compounds and generate valuable products. while escherichia coli has been engineered to produce a variety of fuels and chemicals, it is incapable of ... | 2017 | 28733280 |
| developing a genetically encoded, cross-species biosensor for detecting ammonium and regulating biosynthesis of cyanophycin. | responding to nitrogen status is essential for all living organisms. bacteria have evolved various complex and exquisite regulatory systems to control nitrogen metabolism. however, natural nitrogen regulatory systems, owing to their complexity, often function only in their original hosts and do not respond properly when transferred to another species. by harnessing the lactococcus glnra system, we developed a genetically encoded, cross-species ammonium biosensor that displays a dynamic range up ... | 2017 | 28683543 |
| a novel programmable lysozyme-based lysis system in pseudomonas putida for biopolymer production. | cell lysis is crucial for the microbial production of industrial fatty acids, proteins, biofuels, and biopolymers. in this work, we developed a novel programmable lysis system based on the heterologous expression of lysozyme. the inducible lytic system was tested in two gram-negative bacterial strains, namely escherichia coli and pseudomonas putida kt2440. before induction, the lytic system did not significantly arrest essential physiological parameters in the recombinant e. coli (ecpi) and p. p ... | 2017 | 28663596 |
| de novo biosynthesis of glutarate via α-keto acid carbon chain extension and decarboxylation pathway in escherichia coli. | microbial based bioplastics are promising alternatives to petroleum based synthetic plastics due to their renewability and economic feasibility. glutarate is one of the most potential building blocks for bioplastics. the recent biosynthetic routes for glutarate were mostly based on the l-lysine degradation pathway from pseudomonas putida that required lysine either by feeding or lysine overproduction via genetic manipulations. herein, we established a novel glutarate biosynthetic pathway by inco ... | 2017 | 28618222 |
| interspecies cross-talk between co-cultured pseudomonas putida and escherichia coli. | pseudomonas putida and escherichia coli are ubiquitous microorganisms that can be isolated from soil rhizosphere, the surface of vegetables, fresh waters and wastewaters - environments in which they likely co-exist. despite this, the potential interactions between these microbes have not been studied in detail. to analyse these interactions, we carried out rna-seq transcriptomic analysis of these microbes as monocultures and as co-cultures. our results show that co-culture of these microbes sign ... | 2017 | 28585781 |
| synthetic biology of polyhydroxyalkanoates (pha). | microbial polyhydroxyalkanoates (pha) are a family of biodegradable and biocompatible polyesters which have been extensively studied using synthetic biology and metabolic engineering methods for improving production and for widening its diversity. synthetic biology has allowed pha to become composition controllable random copolymers, homopolymers, and block copolymers. recent developments showed that it is possible to establish a microbial platform for producing not only random copolymers with c ... | 2017 | 28567487 |
| identification of two secondary acyltransferases of lipid a in pseudomonas putida kt2442. | identify the secondary acyltransferases of lipid a in pseudomonas putida. | 2017 | 28557109 |
| mqsr/mqsa toxin/antitoxin system regulates persistence and biofilm formation in pseudomonas putida kt2440. | bacterial toxin/antitoxin (ta) systems have received increasing attention due to their prevalence, diverse structures, and important physiological functions. in this study, we identified and characterized a type ii ta system in a soil bacterium pseudomonas putida kt2440. this ta system belongs to the mqsr/mqsa family. we found that pp_4205 (mqsr) greatly inhibits cell growth in p. putida kt2440 and escherichia coli, the antitoxin pp_4204 (mqsa) neutralizes the toxicity of the toxin mqsr, and the ... | 2017 | 28536573 |
| continuous supply of glucose and glycerol enhances biotransformation of ricinoleic acid to (e)-11-(heptanoyloxy) undec-9-enoic acid in recombinant escherichia coli. | this study aimed at the development of biotransformation strategies with feeding of energy sources for bioconversion of ricinoleic acid to (e)-11-(heptanoyloxy) undec-9-enoic acid (11-houa), a key intermediate of brassylic acid, by recombinant escherichia coli overexpressing an alcohol dehydrogenase from micrococcus luteus and a baeyer-villiger monooxygenase from pseudomonas putida kt2440. feeding of glucose or glycerol facilitated both the preparation of high-density cell biocatalyst and supply ... | 2017 | 28536060 |
| characterisation of a 3-hydroxypropionic acid-inducible system from pseudomonas putida for orthogonal gene expression control in escherichia coli and cupriavidus necator. | 3-hydroxypropionic acid (3-hp) is an important platform chemical used as a precursor for production of added-value compounds such as acrylic acid. metabolically engineered yeast, escherichia coli, cyanobacteria and other microorganisms have been developed for the biosynthesis of 3-hp. attempts to overproduce this compound in recombinant pseudomonas denitrificans revealed that 3-hp is consumed by this microorganism using the catabolic enzymes encoded by genes hpdh, hbdh and mmsa. 3-hp-inducible s ... | 2017 | 28496205 |
| cleaning and disinfection of biofilms composed of listeria monocytogenes and background microbiota from meat processing surfaces. | surfaces of food processing premises are exposed to regular cleaning and disinfection (c&d) regimes, using biocides that are highly effective against bacteria growing as planktonic cells. however, bacteria growing in surface associated communities (biofilms) are typically more tolerant towards c&d than their individual free cells counterparts, and survival of pathogens such as listeria monocytogenes may be affected by interspecies interactions within biofilms. in this study, pseudomonas and acin ... | 2017 | 28667108 |
| the antibacterial activity and mechanism of ginkgolic acid c15:1. | the present study investigated the antibacterial activity and underlying mechanisms of ginkgolic acid (ga) c15:1 monomer using green fluorescent protein (gfp)-labeled bacteria strains. | 2017 | 28088196 |
| metabolic pathway for a new strain pseudomonas synxantha lsh-7': from chemotaxis to uptake of n-hexadecane. | bacteria can use n-hexadecane as a carbon source, but it remains incompletely understood whether n-hexadecane is transformed into metabolic intermediates prior to cellular uptake or not. we newly isolated a strain identified as pseudomonas synxantha lsh-7' and conducted chemotaxis experiment of this bacterial strain towards n-hexadecane, hexadecanol and hexadecanoic acid with qualitative assays respectively. furthermore, we described the identification of extracellular alkane hydroxylase and alc ... | 2017 | 28051099 |
| enhanced heterologous protein productivity by genome reduction in lactococcus lactis nz9000. | the implementation of novel chassis organisms to be used as microbial cell factories in industrial applications is an intensive research field. lactococcus lactis, which is one of the most extensively studied model organisms, exhibits superior ability to be used as engineered host for fermentation of desirable products. however, few studies have reported about genome reduction of l. lactis as a clean background for functional genomic studies and a model chassis for desirable product fermentation ... | 2017 | 28049473 |
| isolation and identification of phyllospheric bacteria possessing antimicrobial activity from astragalus obtusifolius, prosopis juliflora, xanthium strumarium and hippocrepis unisiliqousa. | the widespread utilization of antimicrobial compounds has caused emergence of resistant microorganisms in the world. hence, the research to probe the products with antimicrobial features has led to finding natural habitats and discovering new pharmaceutical products. | 2017 | 28090278 |
| characterization of four new distinct ω-transaminases from pseudomonas putida nbrc 14164 for kinetic resolution of racemic amines and amino alcohols. | four uncharacterized ω-transaminases (ωtas) from pseudomonas putida nbrc 14164 have been identified and cloned from the pool of fully sequenced genomes. the genes were functionally expressed in escherichia coli bl21, and the enzymes were purified and characterized. four tas showed highly (s)-selective ωta activity and converted (s)-α-methylbenzylamine and pyruvate to acetophenone and l-ala. the maximum activity of cloned enzymes was in the ph range of 8.0-8.5 (pp36420), 8.5-9.5 (pp21050), 9.0-9. ... | 2017 | 27714638 |
| engineering gram-negative microbial cell factories using transposon vectors. | the construction of microbial cell factories à la carte largely depends on specialized molecular biology and synthetic biology tools needed to reprogram bacteria for modifying their existing functions or for bestowing them with new-to-nature tasks. in this chapter, we document the use of a series of broad-host-range mini-tn5 vectors for the delivery of gene(s) into the chromosome of gram-negative bacteria and for the generation of saturated, random mutagenesis libraries for studies of gene funct ... | 2017 | 27709582 |
| development of a simplified purification method for a novel formaldehyde dismutase variant from pseudomonas putida j3. | formaldehyde dismutase (fdm) is a very interesting enzyme, due to the fact that it comprises an internal cofactor regeneration mechanism. the fdm, therefore, is able to catalyze redox reactions independent of exogenous cofactor addition, rendering the enzyme powerful for industrial applications. currently, only one enzyme of this type has been characterized enzymatically. furthermore, only one additional dna-sequence with high homology to fdm has been published. in this work, we identified a new ... | 2017 | 27836796 |
| effect of rhamnolipid solubilization on hexadecane bioavailability: enhancement or reduction? | in this study, liquid culture systems containing rhamnolipid-solubilized, separate-phase, and multi-state hexadecane as the carbon source were employed for examining the effect of rhamnolipid solubilization on the bioavailability of hexadecane. experimental results showed that the uptake of rhamnolipid-solubilized hexadecane by pseudomonas aeruginosa atcc 9027, a rhamnolipid producing strain, was enhanced compared to the uptake of mass hexadecane as a separate phase, indicating rhamnolipid solub ... | 2017 | 27773441 |
| fleq of pseudomonas putida kt2440 is a multimeric cyclic diguanylate binding protein that differentially regulates expression of biofilm matrix components. | the intracellular signal molecule cyclic di-gmp (c-di-gmp) is an important element in regulation of biofilm formation by bacteria. in pseudomonas aeruginosa, fleq functions as a c-di-gmp-dependent transcriptional regulator of expression of flagellar genes and the exopolysaccharide (eps) pel, a component of the biofilm extracellular matrix. in the plant-beneficial bacterium pseudomonas putida kt2440, a mutation in fleq reduces biofilm formation and colonization of plant surfaces. using isothermal ... | 2017 | 27503246 |
| bacteria can proliferate in thawed cryoprecipitate stored at room temperature for longer than 4 h. | although key coagulation factor activities are maintained in thawed cryoprecipitate stored for up to 24 h at ambient temperature, several jurisdictions limit such storage to 4-6 h. here, we separately spiked thawed cryoprecipitate units with four bacterial strains: staphylococcus epidermidis, serratia liquefaciens, pseudomonas putida and pseudomonas aeruginosa. no strains grew in the first 4 h of storage, but by 24 h, three of four exhibited up to 1000-fold proliferation. pathogen inactivation t ... | 2017 | 28382701 |
| indole-induced activities of β-lactamase and efflux pump confer ampicillin resistance in pseudomonas putida kt2440. | indole, which is widespread in microbial communities, has received attention because of its effects on bacterial physiology. pseudomonas putida and pseudomonas aeruginosa can acquire ampicillin (amp) resistance during growth on indole-amp agar. transcriptome, mutant, and inhibitor studies have suggested that amp resistance induced by indole can be attributed to increased gene expression of ttgab encoding two genes of rnd-type multidrug efflux operons and an ampc encoding β-lactamase. expression, ... | 2017 | 28352264 |
| dna polymerases imuc and dinb are involved in dna alkylation damage tolerance in pseudomonas aeruginosa and pseudomonas putida. | translesion dna synthesis (tls), facilitated by low-fidelity polymerases, is an important dna damage tolerance mechanism. here, we investigated the role and biological function of tls polymerase imuc (former dnae2), generally present in bacteria lacking dna polymerase v, and tls polymerase dinb in response to dna alkylation damage in pseudomonas aeruginosa and p. putida. we found that tls dna polymerases imuc and dinb ensured a protective role against n- and o-methylation induced by n-methyl-n'- ... | 2017 | 28118378 |
| novel insights into biosynthesis and uptake of rhamnolipids and their precursors. | the human pathogenic bacterium pseudomonas aeruginosa produces rhamnolipids, glycolipids with functions for bacterial motility, biofilm formation, and uptake of hydrophobic substrates. rhamnolipids represent a chemically heterogeneous group of secondary metabolites composed of one or two rhamnose molecules linked to one or mostly two 3-hydroxyfatty acids of various chain lengths. the biosynthetic pathway involves rhamnosyltransferase i encoded by the rhlab operon, which synthesizes 3-(3-hydroxya ... | 2017 | 27988798 |
| the ability of consortium wastewater protozoan and bacterial species to remove cod in the presence of nanomaterials under varying ph conditions. | the aim of this study was to ascertain the survival limit and capability of commonly found wastewater protozoan (aspidisca sp, trachelophyllum sp and peranema sp) and bacterial (bacillus licheniformis, brevibacillus laterosporus and pseudomonas putida) species to remove cod while exposed to commercial nanomaterials under varying ph conditions. the experimental study was carried out in modified mixed liquor media adjusted to various ph levels (ph 2, 7 and 10) and a comparative study was performed ... | 2017 | 28368692 |
| novel tools for the functional expression of metagenomic dna. | functional expression of genes from metagenomic libraries is limited by various factors including inefficient transcription and/or translation of target genes as well as improper folding and assembly of the corresponding proteins caused by the lack of appropriate chaperones and cofactors. it is now well accepted that the use of different expression hosts of distinct phylogeny and physiology can dramatically increase the rate of success. in the following chapter, we therefore describe tools and p ... | 2017 | 27900689 |
| influence of bacterial extracellular polymeric substances on the sorption of zn on γ-alumina: a combination of ftir and exafs studies. | extracellular polymeric substances (eps) isolated from bacteria, are abound of functional groups which can react with metals and consequently influence the immobilization of metals. in this study, we combined with zn k-edge extended x-ray absorption fine structure (exafs), fourier transform infrared (ftir) spectroscopy, and high-resolution transmission electron microscopy (hrtem) techniques to study the effects of eps isolated from bacillus subtilis and pseudomonas putida on zn sorption on γ-alu ... | 2017 | 27876416 |
| inhibited transport of graphene oxide nanoparticles in granular quartz sand coated with bacillus subtilis and pseudomonas putida biofilms. | increasing production and use of graphene oxide nanoparticles (gonps) boost their wide dissemination in the subsurface environments where biofilms occur ubiquitously, representative of the physical and chemical heterogeneities. this study aimed at investigating the influence of gram-positive bacillus subtilis (bs) and gram-negative pseudomonas putida (pp) biofilms on the transport of gonps under different ionic strengths (0.1, 0.5, and 1.0 mm cacl2) at neutral ph 7.2 in water-saturated porous me ... | 2017 | 27855326 |
| aquatic toxicity and biodegradability of a surfactant produced by bacillus subtilis ica56. | in this work, the environmental compatibility of a biosurfactant produced by a bacillus subtilis strain isolated from the soil of a brazilian mangrove was investigated. the biosurfactant, identified as surfactin, is able to reduce surface tension (st) to 31.5 ± 0.1 mn m(-1) and exhibits a lowcritical micelle concentration (cmc) value (0.015 ± 0.003 g l(-1)). the highest crude biosurfactant concentration (224.3 ± 1.9 mg l(-1)) was reached at 72 h of fermentation. acute toxicity tests, carried out ... | 2017 | 27791474 |
| unusual microorganisms and antimicrobial resistances in a group of syrian migrants: sentinel surveillance data from an asylum seekers centre in italy. | three years of civil war in syria have caused death and increase of communicable diseases. the suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps. | 2017 | 26987764 |
| degradation capacities of bacteria and yeasts isolated from the gut of dendroctonus rhizophagus (curculionidae: scolytinae). | bark beetles (curculionidae: scolytinae) feed on the xylem and phloem of their host, which are composed of structural carbohydrates and organic compounds that are not easily degraded by the insects. some of these compounds might be hydrolyzed by digestive enzymes produced by microbes present in the gut of these insects. in this study, we evaluated the enzymatic capacity of bacteria (acinetobacter lwoffii, arthrobacter sp., pseudomonas putida, pseudomonas azotoformans, and rahnella sp.) and yeast ... | 2017 | 27544667 |
| enantioselective resolution of (r,s)-carvedilol to (s)-(-)-carvedilol by biocatalysts. | among the microorganisms employed in the study, aspergillus niger (gufcc5443), escherichia coli (atcc9637), streptomyces halstedii (ckm-2), pseudomonas putida (ncib9494), cunninghamella elegans (ncim689) and sphingomonas paucimobilis (nctc11030) were capable for the enantioselective conversion of racemic carvedilol. immobilization technique enhanced the enantioselectivity of microorganisms and thus increased the enantiomeric purity of the drug. excellent enantiomeric ratios (e) were found in rea ... | 2017 | 28064425 |
| refactoring the embden-meyerhof-parnas pathway as a whole of portable glucobricks for implantation of glycolytic modules in gram-negative bacteria. | the embden-meyerhof-parnas (emp) pathway is generally considered to be the biochemical standard for glucose catabolism. alas, its native genomic organization and the control of gene expression in escherichia coli are both very intricate, which limits the portability of the emp pathway to other biotechnologically important bacterial hosts that lack the route. in this work, the genes encoding all the enzymes of the linear emp route have been individually recruited from the genome of e. coli k-12, ... | 2017 | 28121421 |
| inferring the chemotactic strategy of p. putida and e. coli using modified kramers-moyal coefficients. | many bacteria perform a run-and-tumble random walk to explore their surrounding and to perform chemotaxis. in this article we present a novel method to infer the relevant parameters of bacterial motion from experimental trajectories including the tumbling events. we introduce a stochastic model for the orientation angle, where a shot-noise process initiates tumbles, and analytically calculate conditional moments, reminiscent of kramers-moyal coefficients. matching them with the moments calculate ... | 2017 | 28114420 |
| heterogenic response of prokaryotes toward silver nanoparticles and ions is facilitated by phenotypes and attachment of silver aggregates to cell surfaces. | tons of anthropogenic silver nanoparticles (agnps) are assumed to be released into the environment due to their use in many consumer products. agnps are known to be toxic toward microorganisms and thus may harm their specific functions in ecosystems. here we explore the impact of agnps on functioning of single cells in microbial populations at doses typically found in anthropogenic environments. the response of single cells to agnps was analyzed by flow cytometry and using the fluorescent dyes p ... | 2017 | 28110496 |
| a combined approach for enhancing the stability of recombinant cis-dihydrodiol naphthalene dehydrogenase from pseudomonas putida g7 allowed for the structural and kinetic characterization of the enzyme. | the second enzyme of the naphthalene degradation pathway in pseudomonas putida g7 is nahb, a dehydrogenase that converts cis-1,2-dihydroxy-1,2-dihydronaphthalene to 1,2-dihydroxynaphthalene. we report the cloning, optimization of expression, purification, kinetic studies and preliminary structural characterization of the recombinant nahb. the nahb gene was cloned into a t7 expression vector and the enzyme was overexpressed in escherichia coli rosetta (de3) as an n-terminal hexa-histidine-tagged ... | 2017 | 28089880 |
| maximization of cell viability rather than biocatalyst activity improves whole-cell ω-oxyfunctionalization performance. | it is a common misconception in whole-cell biocatalysis to refer to an enzyme as the biocatalyst, thereby neglecting the structural and metabolic framework provided by the cell. here, the low whole-cell biocatalyst stability, that is, the stability of specific biocatalyst activity, in a process for the terminal oxyfunctionalization of renewable fatty acid methyl esters was investigated. this reaction, which is difficult to achieve by chemical means, is catalyzed by escherichia coli featuring the ... | 2017 | 27883174 |
| breaking down polystyrene through the application of a two-step thermal degradation and bacterial method to produce usable byproducts. | in this work, the objective is to examine a novel approach on the biodegradability of polystyrene is examined. a two-step method for the degradation of polystyrene to chemically useful products has been devised. initially, polystyrene is liquefied via a thermal degradation procedure predominantly into styrene monomers at low temperatures and left to cool down. application of microorganisms in a second step targets the breaking of the polymer into smaller organic molecules. microorganisms tested ... | 2017 | 27440221 |
| seed vectored endophytic bacteria modulate development of rice seedlings. | the aim of the present study was to evaluate the effects of the removal of indigenous bacteria from rice seeds on seedling growth and development. here we report the presence of three indigenous endophytic bacteria in rice seeds that play important roles in modulating seedling development (shoot and root lengths, and formation of root hairs and secondary roots) and defense against pathogens. | 2017 | 28375579 |
| production of polyhydroxyalkanoates (pha) using sludge from different wastewater treatment processes and the potential for medical and pharmaceutical applications. | in this study, seven strains of bacteria with polyhydroxyalkanoates (pha)-producing ability (i.e., bacillus cereus, pseudomonas putida, bacillus pumilus, pseudomona-huttiensis, yersinia-frederiksenii, aeromonas ichthiosmia and sphingopyxis terrae) were isolated from various waste treatment plants in hong kong. simultaneous wastewater treatment and pha accumulation were successfully achieved in the bioreactors using isolated bacteria from different sludges. at the organic loading less than 13000 ... | 2017 | 28387154 |
| the n-acetylmuramic acid 6-phosphate phosphatase mupp completes the pseudomonas peptidoglycan recycling pathway leading to intrinsic fosfomycin resistance. | bacterial cells are encased in and stabilized by a netlike peptidoglycan (pgn) cell wall that undergoes turnover during bacterial growth. pgn turnover fragments are frequently salvaged by the cells via a pathway referred to as pgn recycling. two different routes for the recycling of the cell wall sugar n-acetylmuramic acid (murnac) have been recognized in bacteria. in escherichia coli and related enterobacteria, as well as in most gram-positive bacteria, murnac is recovered via a catabolic route ... | 2017 | 28351914 |
| identification and functional analysis of the activator gene involved in the biosynthesis of co-type nitrile hydratase from aurantimonas manganoxydans. | nitrile hydratase (nhase) has attracted considerable attention to synthesize valuable amides. here, the gene cluster of co-type nhase from aurantimonas manganoxydans atcc baa-1229 (nhase1229) was identified through edman degradation and recombinant expression, including α-subunit, β-subunit, and activator. the activator gene contained 408 bases, which was essential for the biosynthesis of nhase1229 in escherichia coli. the activator gene can be substituted by co-type activator from pseudonocardi ... | 2017 | 28323014 |
| expansion of the ω-oxidation system alkbgtl of pseudomonas putida gpo1 with alkj and alkh results in exclusive mono-esterified dicarboxylic acid production in e. coli. | the alkbgtl proteins coded on the alk operon from pseudomonas putida gpo1 can selectively ω-oxidize ethyl esters of c6 to c10 fatty acids in whole-cell conversions with escherichia coli. the major product in these conversions is the ω-alcohol. however, alkb also has the capacity to overoxidize the substrate to the ω-aldehyde and ω-acid. in this study, we show that alcohol dehydrogenase alkj and aldehyde dehydrogenase alkh are able to oxidize ω-alcohols and ω-aldehydes of esterified fatty acids r ... | 2017 | 28321989 |
| expression of heterologous sigma factor expands the searchable space for biofuel tolerance mechanisms. | microorganisms can produce hydrocarbons that can serve as replacements or additions to conventional liquid fuels for use in the transportation sector. however, a common problem in the microbial synthesis of biofuels is that these compounds often have toxic effects on the cell. in this study, we focused on mitigating the toxicity of the biojet fuel precursor pinene on escherichia coli. we used genomic dna from pseudomonas putida kt2440, which has innate solvent-tolerance properties, to create tra ... | 2017 | 28319371 |
| the xyls/pm regulator/promoter system and its use in fundamental studies of bacterial gene expression, recombinant protein production and metabolic engineering. | the xyls/pm regulator/promoter system originating from the pseudomonas putida tol plasmid pww0 is widely used for regulated low- and high-level recombinant expression of genes and gene clusters in escherichia coli and other bacteria. induction of this system can be graded by using different cheap benzoic acid derivatives, which enter cells by passive diffusion, operate in a dose-dependent manner and are typically not metabolized by the host cells. combinatorial mutagenesis and selection using th ... | 2017 | 28276630 |
| identification and characterization of a novel three-component phenazine-1-carboxylic acid 1, 2-dioxygenase in sphingomonas wittichii dp58. | phenazine-1-carboxylic acid, the main component of shenqinmycin, is widely used in southern china for the prevention of rice sheath blight. however, the fate of phenazine-1-carboxylic acid in soil remains uncertain. s. wittichii dp58 can use phenazine-1-carboxylic acid as its sole carbon and nitrogen sources for growth. in this study, dioxygenase-encoding genes, pcaa1a2, were found using transcriptome analysis to be highly upregulated upon phenazine-1-carboxylic acid biodegradation. pcaa1 shares ... | 2017 | 28188209 |
| risk of infection transmission in curvilinear array echoendoscopes: results of a prospective reprocessing and culture registry. | the complex design of the elevator mechanism in duodenoscopes has been recognized as a challenge for disinfection and recently implicated as a potential source of persistent bacterial contamination. curvilinear array (cla) echoendoscopes also have an elevator mechanism; however, there are no recommendations or data regarding the risk of persistent bacterial contamination of echoendoscopes. here we hoped to determine the yield of microbial growth with routine bacterial surveillance cultures of re ... | 2017 | 27478029 |
| simplified and representative bacterial community of maize roots. | plant-associated microbes are important for the growth and health of their hosts. as a result of numerous prior studies, we know that host genotypes and abiotic factors influence the composition of plant microbiomes. however, the high complexity of these communities challenges detailed studies to define experimentally the mechanisms underlying the dynamics of community assembly and the beneficial effects of such microbiomes on plant hosts. in this work, from the distinctive microbiota assembled ... | 2017 | 28275097 |