Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| heme signaling impacts global gene expression, immunity and dengue virus infectivity in aedes aegypti. | blood-feeding mosquitoes are exposed to high levels of heme, the product of hemoglobin degradation. heme is a pro-oxidant that influences a variety of cellular processes. we performed a global analysis of heme-regulated aedes aegypti (yellow fever mosquito) transcriptional changes to better understand influence on mosquito physiology at the molecular level. we observed an iron- and reactive oxygen species (ros)-independent signaling induced by heme that comprised genes related to redox metabolis ... | 2015 | 26275150 |
| high throughput profiling of the cotton bollworm helicoverpa armigera immunotranscriptome during the fungal and bacterial infections. | innate immunity is essential in defending against invading pathogens in invertebrates. the cotton bollworm, helicoverpa armigera (hübner) is one of the most destructive lepidopteran pests, which causes enormous economic losses in agricultural production worldwide. the components of the immune system are largely unknown in this insect. the application of entomopathogens is considered as an alternative to the chemical insecticides for its control. however, few studies have focused on the molecular ... | 2015 | 26001831 |
| research on bacterial virulence in the developing countries. | 2015 | 25713813 | |
| bilateral conjunctivitis in a returned traveller. | 2015 | 25590460 | |
| of elic and evolution. | 2015 | 25548134 | |
| insect-specific virus discovery: significance for the arbovirus community. | arthropod-borne viruses (arboviruses), especially those transmitted by mosquitoes, are a significant cause of morbidity and mortality in humans and animals worldwide. recent discoveries indicate that mosquitoes are naturally infected with a wide range of other viruses, many within taxa occupied by arboviruses that are considered insect-specific. over the past ten years there has been a dramatic increase in the literature describing novel insect-specific virus detection in mosquitoes, which has p ... | 2015 | 26378568 |
| insect-specific flaviviruses: a systematic review of their discovery, host range, mode of transmission, superinfection exclusion potential and genomic organization. | there has been a dramatic increase in the number of insect-specific flaviviruses (isfs) discovered in the last decade. historically, these viruses have generated limited interest due to their inability to infect vertebrate cells. this viewpoint has changed in recent years because some isfs have been shown to enhance or suppress the replication of medically important flaviviruses in co-infected mosquito cells. additionally, comparative studies between isfs and medically important flaviviruses can ... | 2015 | 25866904 |
| midgut immune responses induced by bacterial infection in the silkworm, bombyx mori. | insect gut epithelial cells produce reactive oxygen species (ros) and antimicrobial peptides (amps) to protect hosts from pathogenic microorganisms. in this study, we evaluate the pathogenicity of pseudomonas aeruginosa and bacillus bombysepticus in the silkworm, bombyx mori. survival curves show that b. bombysepticus is deadly when larval silkworms are infected orally. bacterial infection caused intestinal hydrogen peroxide (h2o2) and nitric oxide (no) levels to increase significantly by 8 and ... | 2015 | 26465135 |
| drosophila innate immunity: regional and functional specialization of prophenoloxidases. | the diversification of immune systems during evolution involves the expansion of particular gene families in given phyla. a better understanding of the metazoan immune system requires an analysis of the logic underlying such immune gene amplification. this analysis is now within reach due to the ease with which we can generate multiple mutations in an organism. in this paper, we analyze the contribution of the three drosophila prophenoloxidases (ppos) to host defense by generating single, double ... | 2015 | 26437768 |
| dengue virus infection of aedes aegypti requires a putative cysteine rich venom protein. | dengue virus (denv) is a mosquito-borne flavivirus that causes serious human disease and mortality worldwide. there is no specific antiviral therapy or vaccine for denv infection. alterations in gene expression during denv infection of the mosquito and the impact of these changes on virus infection are important events to investigate in hopes of creating new treatments and vaccines. we previously identified 203 genes that were ≥5-fold differentially upregulated during flavivirus infection of the ... | 2015 | 26491875 |
| an insight into the functional role of thioredoxin reductase, a selenoprotein, in maintaining normal native microbiota in the gulf coast tick (amblyomma maculatum). | tick selenoproteins have been associated with antioxidant activity in ticks. thioredoxin reductase (trxr), also a selenoprotein, belongs to the pyridine nucleotide-disulphide oxidoreductase family of proteins and is an important antioxidant. molecular interactions between native microbiota and tick hosts have barely been investigated to date. in this study, we determined the functional role of trxr in tick feeding and in maintenance of the native microbial community. trxr transcript levels remai ... | 2015 | 26184979 |
| nuclease tudor-sn is involved in tick dsrna-mediated rna interference and feeding but not in defense against flaviviral or anaplasma phagocytophilum rickettsial infection. | tudor staphylococcal nuclease (tudor-sn) and argonaute (ago) are conserved components of the basic rna interference (rnai) machinery with a variety of functions including immune response and gene regulation. the rnai machinery has been characterized in tick vectors of human and animal diseases but information is not available on the role of tudor-sn in tick rnai and other cellular processes. our hypothesis is that tick tudor-sn is part of the rnai machinery and may be involved in innate immune r ... | 2015 | 26186700 |
| movement of regulatory rna between animal cells. | recent studies suggest that rna can move from one cell to another and regulate genes through specific base-pairing. mechanisms that modify or select rna for secretion from a cell are unclear. secreted rna can be stable enough to be detected in the extracellular environment and can enter the cytosol of distant cells to regulate genes. mechanisms that import rna into the cytosol of an animal cell can enable uptake of rna from many sources including other organisms. this role of rna is akin to that ... | 2015 | 26138457 |
| infectious uveitis. | infectious uveitis is one of the most common and visually devastating causes of uveitis in the us and worldwide. this review provides a summary of the identification, treatment, and complications associated with certain forms of viral, bacterial, fungal, helminthic, and parasitic uveitis. in particular, this article reviews the literature on identification and treatment of acute retinal necrosis due to herpes simplex virus, varicella virus, and cytomegalovirus. while no agreed-upon treatment has ... | 2015 | 26618074 |
| abstracts from the 38th annual meeting of the society of general internal medicine. | 2015 | 25869016 | |
| anopheles midgut epithelium evades human complement activity by capturing factor h from the blood meal. | hematophagous vectors strictly require ingesting blood from their hosts to complete their life cycles. exposure of the alimentary canal of these vectors to the host immune effectors necessitates efficient counteractive measures by hematophagous vectors. the anopheles mosquito transmitting the malaria parasite is an example of hematophagous vectors that within seconds can ingest human blood double its weight. the innate immune defense mechanisms, like the complement system, in the human blood sho ... | 2015 | 25679788 |
| developmental effects of ionizing radiation in aedes aegypti. | 2015 | 5080436 | |
| prolyl oligopeptidase from the blood fluke schistosoma mansoni: from functional analysis to anti-schistosomal inhibitors. | blood flukes of the genus schistosoma cause schistosomiasis, a parasitic disease that infects over 240 million people worldwide, and for which there is a need to identify new targets for chemotherapeutic interventions. our research is focused on schistosoma mansoni prolyl oligopeptidase (smpop) from the serine peptidase family s9, which has not been investigated in detail in trematodes. | 2015 | 26039195 |
| [effects of 2 antimetabolites on the development of aedes aegypti larvae]. | 2015 | 4662010 | |
| paratransgenesis: a promising new strategy for mosquito vector control. | the three main mosquito genera, anopheles, aedes and culex, transmit respectively malaria, dengue and lymphatic filariasis. current mosquito control strategies have proved unsuccessful, and there still is a substantial number of morbidity and mortality from these diseases. genetic control methods have now arisen as promising alternative strategies, based on two approaches: the replacement of a vector population by disease-refractory mosquitoes and the release of mosquitoes carrying a lethal gene ... | 2015 | 26104575 |
| aerobic bacterial flora of biotic and abiotic compartments of a hyperendemic zoonotic cutaneous leishmaniasis (zcl) focus. | identification of the microflora of the sand fly gut and the environmental distribution of these bacteria are important components for paratransgenic control of leishmania transmission by sand flies. | 2015 | 25630498 |
| bacterial diversity associated with wild caught anopheles mosquitoes from dak nong province, vietnam using culture and dna fingerprint. | microbiota of anopheles midgut can modulate vector immunity and block plasmodium development. investigation on the bacterial biodiversity in anopheles, and specifically on the identification of bacteria that might be used in malaria transmission blocking approaches, has been mainly conducted on malaria vectors of africa. vietnam is an endemic country for both malaria and bancroftian filariasis whose parasitic agents can be transmitted by the same anopheles species. no information on the microbio ... | 2015 | 25747513 |
| the waal gene mutation compromised the inhabitation of enterobacter sp. ag1 in the mosquito gut environment. | the mosquito gut harbors a variety of bacteria that are dynamically associated with mosquitoes in various contexts. however, little is known about bacterial factors that affect bacterial inhabitation in the gut microbial community. enterobacter sp. ag1 is a predominant gram negative bacterium in the mosquito midgut. | 2015 | 26306887 |
| the dimerization state of the mammalian high mobility group protein at-hook 2 (hmga2). | the mammalian high mobility group protein at-hook 2 (hmga2) is a chromosomal architectural transcription factor involved in cell transformation and oncogenesis. it consists of three positively charged "at-hooks" and a negatively charged c-terminus. sequence analyses, circular dichroism experiments, and gel-filtration studies showed that hmga2, in the native state, does not have a defined secondary or tertiary structure. surprisingly, using combined approaches of 1-ethyl-3-(3-dimethylaminopropyl) ... | 2015 | 26114780 |
| oviposition responses of aedes mosquitoes to bacterial isolates from attractive bamboo infusions. | the mosquitoes aedes aegypti and aedes albopictus are vectors of pathogenic viruses that cause major human illnesses including dengue, yellow fever and chikungunya. both mosquito species are expanding their geographic distributions and now occur worldwide in temperate and tropical climates. collection of eggs in oviposition traps (ovitraps) is commonly used for monitoring and surveillance of container-inhabiting aedes populations by public health agencies charged with managing mosquito-transmitt ... | 2015 | 26399712 |
| the wolbachia wo bacteriophage proteome in the aedes albopictus c/wstr1 cell line: evidence for lytic activity? | wolbachia pipientis (rickettsiales), an obligate intracellular alphaproteobacterium in insects, manipulates host reproduction to maximize invasion of uninfected insect populations. modification of host population structure has potential applications for control of pest species, particularly if wolbachia can be maintained, manipulated, and genetically engineered in vitro. although wolbachia maintains an obligate mutualism with genome stability in nematodes, arthropods can be co-infected with dist ... | 2015 | 26427709 |
| nanoparticles as potential new generation broad spectrum antimicrobial agents. | the rapid emergence of antimicrobial resistant strains to conventional antimicrobial agents has complicated and prolonged infection treatment and increased mortality risk globally. furthermore, some of the conventional antimicrobial agents are unable to cross certain cell membranes thus, restricting treatment of intracellular pathogens. therefore, the disease-causing-organisms tend to persist in these cells. however, the emergence of nanoparticle (np) technology has come with the promising broad ... | 2015 | 26329777 |
| non-specific esterases in several organs of aedes aegypti (l.) during female adult life. | 2015 | 4709581 | |
| the genome of diuraphis noxia, a global aphid pest of small grains. | the russian wheat aphid, diuraphis noxia kurdjumov, is one of the most important pests of small grains throughout the temperate regions of the world. this phytotoxic aphid causes severe systemic damage symptoms in wheat, barley, and other small grains as a direct result of the salivary proteins it injects into the plant while feeding. | 2015 | 26044338 |
| mutualism breakdown by amplification of wolbachia genes. | most insect species are associated with vertically transmitted endosymbionts. because of the mode of transmission, the fitness of these symbionts is dependent on the fitness of the hosts. therefore, these endosymbionts need to control their proliferation in order to minimize their cost for the host. the genetic bases and mechanisms of this regulation remain largely undetermined. the maternally inherited bacteria of the genus wolbachia are the most common endosymbionts of insects, providing some ... | 2015 | 25668031 |
| invasion of aedes albopictus (diptera: culicidae) into central africa: what consequences for emerging diseases? | aedes albopictus, a mosquito native to asia, has invaded all five continents during the past three decades. it was reported in central africa in the 2000s, first in cameroon, and, since then, has colonised almost all countries of the region. the species, originally considered a secondary vector of dengue viruses, has been showed to play a major role in transmission of chikungunya virus in numerous countries, including in the central african region. we review the current spread of ae. albopictus ... | 2015 | 25885461 |
| the first imported case infected with chikungunya virus in korea. | chikungunya is caused by an arbovirus transmitted by aedes mosquito vector. with the increase of habitat of mosquito by global warming and frequent international travel and interchange, chikungunya reemerged and showed global distribution recently. until now there has not been reported any case infected with chikungunya virus in korea. a 23-year-old man has been the republic of the philippines for 1 week, and visited our emergency center due to fever and back pain. chikungunya viral infection wa ... | 2015 | 25844264 |
| proteomics profiling of chikungunya-infected aedes albopictus c6/36 cells reveal important mosquito cell factors in virus replication. | chikungunya virus (chikv) is the only causative agent of chikv fever with persistent arthralgia, and in some cases may lead to neurological complications which can be highly fatal, therefore it poses severe health issues in many parts of the world. chikv transmission can be mediated via the aedes albopictus mosquito; however, very little is currently known about the involvement of mosquito cellular factors during chikv-infection within the mosquito cells. unravelling the neglected aspects of mos ... | 2015 | 25738640 |
| a multipurpose, high-throughput single-nucleotide polymorphism chip for the dengue and yellow fever mosquito, aedes aegypti. | the dengue and yellow fever mosquito, aedes aegypti, contributes significantly to global disease burden. genetic study of aedes aegypti is essential to understanding its evolutionary history, competence as a disease vector, and the effects and efficacy of vector control methods. the prevalence of repeats and transposable elements in the aedes aegypti genome complicates marker development and makes genome-wide genetic study challenging. to overcome these challenges, we developed a high-throughput ... | 2015 | 25721127 |
| a case of diabetic ketoacidosis following chikungunya virus infection. | chikungunya is a mosquito-borne viral disease that has recently become endemic in the caribbean, including the island of puerto rico. we present the case of a 50-year-old puerto rican man who traveled to st. louis for business and was diagnosed with acute chikungunya virus infection with atypical features causing diabetic ketoacidosis. this case highlights the need to keep tropical infectious diseases on the differential diagnosis in appropriate individuals and the ways in which tropical infecti ... | 2015 | 26033023 |
| influence of breeding site availability on the oviposition behaviour of aedes aegypti. | despite the importance of the mosquito aedes aegypti in the transmission of arboviruses, such as yellow fever, chikungunya fever and dengue fever, some aspects of their behaviour remain unknown. in the present study, the oviposition behaviour of ae. aegypti females that were exposed to different densities of breeding sites (2, 4, 8 and 16) was evaluated in laboratory and semi-field conditions. the number of breeding sites that were used was proportional to the number available, but tended toward ... | 2015 | 26154742 |
| complete genome sequence of chikungunya virus isolated from an aedes aegypti mosquito during an outbreak in yemen, 2011. | chikungunya virus is recognized as a serious public health problem. the complete genome was sequenced for a chikungunya virus isolated from the mosquito aedes aegypti during a 2011 outbreak in al hodayda, yemen, which resulted in significant human fatalities. phylogenetic analysis demonstrated that this yemeni isolate is most closely related to indian ocean strains of the east/central/south african genotype. | 2015 | 26184944 |
| an outbreak of chikungunya in rural bangladesh, 2011. | the first identified chikungunya outbreak occurred in bangladesh in 2008. in late october 2011, a local health official from dohar sub-district, dhaka district, reported an outbreak of undiagnosed fever and joint pain. we investigated the outbreak to confirm the etiology, describe the clinical presentation, and identify associated vectors. | 2015 | 26161995 |
| differential analysis of the secretome of wrl68 cells infected with the chikungunya virus. | the chikungunya virus (chikv) is an arthropod borne virus. in the last 50 years, it has been the cause of numerous outbreaks in tropical and temperate regions, worldwide. there is limited understanding regarding the underlying molecular mechanisms involved in chikv replication and how the virus interacts with its host. in the present study, comparative proteomics was used to identify secreted host proteins that changed in abundance in response to early chikv infection. two-dimensional gel electr ... | 2015 | 26083627 |
| caribbean and la réunion chikungunya virus isolates differ in their capacity to induce proinflammatory th1 and nk cell responses and acute joint pathology. | chikungunya virus (chikv) is a mosquito-borne arthralgic alphavirus that has garnered international attention as an important emerging pathogen since 2005. more recently, it invaded the caribbean islands and the western hemisphere. intriguingly, the current chikv outbreak in the caribbean is caused by the asian chikv genotype, which differs from the la réunion lr2006 opy1 isolate belonging to the indian ocean lineage. here, we adopted a systematic and comparative approach against lr2006 opy1 to ... | 2015 | 25995257 |
| chikungunya virus transmission potential by local aedes mosquitoes in the americas and europe. | chikungunya virus (chikv), mainly transmitted in urban areas by the mosquitoes aedes aegypti and aedes albopictus, constitutes a major public health problem. in late 2013, chikv emerged on saint-martin island in the caribbean and spread throughout the region reaching more than 40 countries. thus far, ae. aegypti mosquitoes have been implicated as the sole vector in the outbreaks, leading to the hypothesis that chikv spread could be limited only to regions where this mosquito species is dominant. | 2015 | 25993633 |
| a combination of doxycycline and ribavirin alleviated chikungunya infection. | lack of vaccine and effective antiviral drugs against chikungunya virus (chikv) outbreaks have led to significant impact on health care in the developing world. here, we evaluated the antiviral effects of tetracycline (tetra) derivatives and other common antiviral agents against chikv. our results showed that within the tetra derivatives group, doxycycline (doxy) exhibited the highest inhibitory effect against chikv replication in vero cells. on the other hand, in the antiviral group ribavirin ( ... | 2015 | 25970853 |
| high rate of subclinical chikungunya virus infection and association of neutralizing antibody with protection in a prospective cohort in the philippines. | chikungunya virus (chikv) is a globally re-emerging arbovirus for which previous studies have indicated the majority of infections result in symptomatic febrile illness. we sought to characterize the proportion of subclinical and symptomatic chikv infections in a prospective cohort study in a country with known chikv circulation. | 2015 | 25951202 |
| seroepidemiological survey of chikungunya in and around the regions of bijapur (vijayapura - north karnataka). | chikungunya is a debilitating, non-fatal, mosquito borne viral fever caused by chikungunya virus (chiva). the disease is transmitted to humans by the bite of aedes aegypti and aedes albopictus mosquitoes. severe outbreaks of chikungunya have been reported in several countries of africa and asia. chikungunya fever is characterized by fever with sudden onset, arthralgia, rash, headache and myalgia. however, arthralgia is painful and long-lasting, affecting primarily the peripheral joints. | 2015 | 26155474 |
| small mosquitoes, large implications: crowding and starvation affects gene expression and nutrient accumulation in aedes aegypti. | environmental factors such as temperature, nutrient availability, and larval density determine the outcome of postembryonic development in mosquitoes. suboptimal temperatures, crowding, and starvation during the larval phase reduce adult mosquito size, nutrient stores and affect vectorial capacity. | 2015 | 25924822 |
| socio-economic and climate factors associated with dengue fever spatial heterogeneity: a worked example in new caledonia. | understanding the factors underlying the spatio-temporal distribution of infectious diseases provides useful information regarding their prevention and control. dengue fever spatio-temporal patterns result from complex interactions between the virus, the host, and the vector. these interactions can be influenced by environmental conditions. our objectives were to analyse dengue fever spatial distribution over new caledonia during epidemic years, to identify some of the main underlying factors, a ... | 2015 | 26624008 |
| potential of selected senegalese aedes spp. mosquitoes (diptera: culicidae) to transmit zika virus. | zika virus (zikv; genus flavivirus, family flaviviridae) is an emerging virus of medical importance maintained in a zoonotic cycle between arboreal aedes spp. mosquitoes and nonhuman primates in african and asian forests. serological evidence and virus isolations have demonstrated widespread distribution of the virus in senegal. several mosquito species have been found naturally infected by zikv but little is known about their vector competence. | 2015 | 26527535 |
| short-term suppression of aedes aegypti using genetic control does not facilitate aedes albopictus. | under permit from the national biosafety commission for the use of genetically modified organisms, releases of a genetically engineered self-limiting strain of aedes aegypti (ox513a) were used to suppress urban pest ae. aegypti in west panama. experimental goals were to assess the effects on a coexisting population of ae. albopictus and examine operational parameters with relevance to environmental impact. | 2015 | 26374668 |
| detection of chikungunya virus in nepal. | chikungunya virus (chikv) is an emerging alphaviral disease and a public health problem in south asia including nepal in recent years. in this study, sera were collected from patients presenting with fever, headache, muscular pain, fatigue, and joint pain of both upper and lower extremities. a total of 169 serum samples were tested for chikv and dengue virus (denv) by using immunoglobulin m (igm) and immunoglobulin g (igg) antibody using enzyme-linked immunosorbent assay (elisa) method during au ... | 2015 | 26195462 |
| psychiatric morbidity in patients with chikungunya fever: first report from india. | chikungunya fever is an acute illness caused by an arbovirus and has various complications like neurological, psychological, dermatological and even multi organ failure. psychiatric co-morbidity is not very well studied till now. this is the first report from india. | 2015 | 26557595 |
| french invasive asian tiger mosquito populations harbor reduced bacterial microbiota and genetic diversity compared to vietnamese autochthonous relatives. | the asian tiger mosquito aedes albopictus is one of the most significant pathogen vectors of the twenty-first century. originating from asia, it has invaded a wide range of eco-climatic regions worldwide. the insect-associated microbiota is now recognized to play a significant role in host biology. while genetic diversity bottlenecks are known to result from biological invasions, the resulting shifts in host-associated microbiota diversity has not been thoroughly investigated. to address this su ... | 2015 | 26441903 |
| the chikungunya epidemic: a look at five cases. | chikungunya is an infection caused by the chikungunya virus and transmitted by the bite of infected mosquito. the most common symptoms of chikungunya virus infection are fever, joint pain or rash. chikungunya virus outbreaks had been identified in countries in africa, asia, europe, and the indian and pacific oceans. in late 2013, the first local transmission of chikungunya virus in the americas was identified in caribbean countries and territories. chikungunya virus disease became a nationally n ... | 2015 | 26793467 |
| fitness cost in field and laboratory aedes aegypti populations associated with resistance to the insecticide temephos. | the continued use of chemical insecticides in the context of the national program of dengue control in brazil has generated a high selective pressure on the natural populations of aedes aegypti, leading to their resistance to these compounds in the field. fitness costs have been described as adaptive consequences of resistance. this study evaluated the biological and reproductive performance of a. aegypti strains and a field population resistant to temephos, the main larvicide used for controlli ... | 2015 | 26715037 |
| correlation of clinicohaematological parameters in paediatric dengue: a retrospective study. | dengue is one of the arthropod-borne (arbo) viral diseases transmitted by female mosquito aedes aegypti. dengue fever has a wide spectrum of clinical presentation ranging from flu-like illness to severe complicated stage of dengue hemorrhagic fever leading to mortality. this was a retrospective study conducted in a tertiary care hospital in coastal karnataka, south india, to know the correlation between the clinical presentation and haematological parameters in the paediatric cases presented wit ... | 2015 | 26819620 |
| peridomestic infection as a determining factor of dengue transmission. | the study of endemic dengue transmission is essential for proposing alternatives to impact its burden. the traditional paradigm establishes that transmission starts around cases, but there are few studies that determine the risk. | 2015 | 26671573 |
| human social behavior and demography drive patterns of fine-scale dengue transmission in endemic areas of colombia. | dengue is known to transmit between humans and a. aegypti mosquitoes living in neighboring houses. although transmission is thought to be highly heterogeneous in both space and time, little is known about the patterns and drivers of transmission in groups of houses in endemic settings. we carried out surveys of pcr positivity in children residing in 2-block patches of highly endemic cities of colombia. we found high levels of heterogeneity in pcr positivity, varying from less than 30% in 8 of th ... | 2015 | 26656072 |
| assessing the presence of wuchereria bancrofti in vector and human populations from urban communities in conakry, guinea. | the global programme to eliminate lymphatic filariasis was launched in 2000 with the goal of interrupting transmission of lymphatic filariasis (lf) through multiple rounds of mass drug administration (mda). in guinea, there is evidence of ongoing lf transmission, but little is known about the most densely populated parts of the country, including the capital conakry. in order to guide the lf control and elimination efforts, serological and entomological surveys were carried out to determine whet ... | 2015 | 26410739 |
| annona muricata (annonaceae): a review of its traditional uses, isolated acetogenins and biological activities. | annona muricata is a member of the annonaceae family and is a fruit tree with a long history of traditional use. a. muricata, also known as soursop, graviola and guanabana, is an evergreen plant that is mostly distributed in tropical and subtropical regions of the world. the fruits of a. muricata are extensively used to prepare syrups, candies, beverages, ice creams and shakes. a wide array of ethnomedicinal activities is contributed to different parts of a. muricata, and indigenous communities ... | 2015 | 26184167 |
| interaction of a dengue virus ns1-derived peptide with the inhibitory receptor kir3dl1 on natural killer cells. | killer immunoglobulin-like receptors (kirs) interact with human leucocyte antigen (hla) class i ligands and play a key role in the regulation and activation of nk cells. the functional importance of kir-hla interactions has been demonstrated for a number of chronic viral infections, but to date only a few studies have been performed in the context of acute self-limited viral infections. during our investigation of cd8(+) t cell responses to a conserved hla-b57-restricted epitope derived from den ... | 2015 | 26439909 |
| production of infectious dengue virus in aedes aegypti is dependent on the ubiquitin proteasome pathway. | dengue virus (denv) relies on host factors to complete its life cycle in its mosquito host for subsequent transmission to humans. denv first establishes infection in the midgut of aedes aegypti and spreads to various mosquito organs for lifelong infection. curiously, studies have shown that infectious denv titers peak and decrease thereafter in the midgut despite relatively stable viral genome levels. however, the mechanisms that regulate this decoupling of infectious virion production from vira ... | 2015 | 26566123 |
| a re-examination of the history of etiologic confusion between dengue and chikungunya. | contrary to the perception of many researchers that the recent invasion of chikungunya (chik) in the western hemisphere marked the first episode in history, a recent publication reminded them that chik had prevailed in the west indies and southern regions of the united states from 1827-1828 under the guise of "dengue" (den), and that many old outbreaks of so-called "dengue" actually represented the chik cases erroneously identified as "dengue." in hindsight, this confusion was unavoidable, given ... | 2015 | 26562299 |
| dengue virus infection alters post-transcriptional modification of micrornas in the mosquito vector aedes aegypti. | recent discoveries regarding the importance of isomirs have increased our understanding of the regulatory complexities of the mirnaome. observed changes in the mirna profiles in mosquitoes infected with flaviviruses have implicated small rnas in the interactions between viruses and their vectors. here we analysed the isomir profiles of both uninfected and infected aedes aegypti mosquitoes with the major human pathogen dengue virus (denv). we found that several specific isomirs were significantly ... | 2015 | 26514826 |
| the effects of socioeconomic and environmental factors on the incidence of dengue fever in the pearl river delta, china, 2013. | an outbreak of dengue fever (df) occurred in guangdong province, china in 2013 with the highest number of cases observed within the preceding ten years. df cases were clustered in the pearl river delta economic zone (prd) in guangdong province, which accounted for 99.6% of all cases in guangdong province in 2013. the main vector in prd was aedes albopictus. we investigated the socioeconomic and environmental factors at the township level and explored how the independent variables jointly affect ... | 2015 | 26506616 |
| wolbachia and the insect immune system: what reactive oxygen species can tell us about the mechanisms of wolbachia-host interactions. | wolbachia are intracellular bacteria that infect a vast range of arthropod species, making them one of the most prevalent endosymbionts in the world. wolbachia's stunning evolutionary success is mostly due to their reproductive parasitism but also to mutualistic effects such as increased host fecundity or protection against pathogens. however, the mechanisms underlying wolbachia phenotypes, both parasitic and mutualistic, are only poorly understood. moreover, it is unclear how the insect immune ... | 2015 | 26579107 |
| copulation activity, sperm production and conidia transfer in aedes aegypti males contaminated by metarhizium anisopliae: a biological control prospect. | dengue is the most prevalent arboviral disease transmitted by aedes aegypti worldwide, whose chemical control is difficult, expensive, and of inconsistent efficacy. releases of metarhizium anisopliae--exposed ae. aegypti males to disseminate conidia among female mosquitoes by mating represents a promising biological control approach against this important vector. a better understanding of fungus virulence and impact on reproductive parameters of ae. aegypti, is need before testing auto-dissemina ... | 2015 | 26473490 |
| economic burden of dengue virus infection at the household level among residents of puerto maldonado, peru. | dengue virus (denv) was reintroduced to peru in the 1990s and has been reported in puerto maldonado (population ~65,000) in the peruvian southern amazon basin since 2000. this region also has the highest human migration rate in the country, mainly from areas not endemic for denv. the objective of this study was to assess the proportion of household income that is diverted to costs incurred because of dengue illness and to compare these expenses between recent migrants (rms) and long-term residen ... | 2015 | 26217040 |
| region-wide synchrony and traveling waves of dengue across eight countries in southeast asia. | dengue is a mosquito-transmitted virus infection that causes epidemics of febrile illness and hemorrhagic fever across the tropics and subtropics worldwide. annual epidemics are commonly observed, but there is substantial spatiotemporal heterogeneity in intensity. a better understanding of this heterogeneity in dengue transmission could lead to improved epidemic prediction and disease control. time series decomposition methods enable the isolation and study of temporal epidemic dynamics with a s ... | 2015 | 26438851 |
| widespread signatures of local mrna folding structure selection in four dengue virus serotypes. | it is known that mrna folding can affect and regulate various gene expression steps both in living organisms and in viruses. previous studies have recognized functional rna structures in the genome of the dengue virus. however, these studies usually focused either on the viral untranslated regions or on very specific and limited regions at the beginning of the coding sequences, in a limited number of strains, and without considering evolutionary selection. | 2015 | 26449467 |
| a survey of the 2014 dengue fever epidemic in guangzhou, china. | in 2014, a serious dengue outbreak in guangzhou occurred, consisting of 37 354 laboratory confirmed cases of infection. in this study, the clinical picture of dengue fever due to dengue virus (denv) type 1 in guangzhou was described. clinical and laboratory data collected by studying 726 sera of suspected clinical cases from hospitals and 328 sera of healthy persons from two residence communities were analyzed during the outbreak, and 484 patients were diagnosed with an acute dengue infection. f ... | 2015 | 26954995 |
| gene expression changes in the salivary glands of anopheles coluzzii elicited by plasmodium berghei infection. | malaria is a devastating infectious disease caused by plasmodium parasites transmitted through the bites of infected anopheles mosquitoes. salivary glands are the only mosquito tissue invaded by plasmodium sporozoites, being a key stage for the effective parasite transmission, making the study of anopheles sialome highly relevant. | 2015 | 26395987 |
| a dengue outbreak on a floating village at cat ba island in vietnam. | a dengue outbreak in an ecotourism destination spot in vietnam, from september to november 2013, impacted a floating village of fishermen on the coastal island of cat ba. the outbreak raises questions about how tourism may impact disease spread in rural areas. | 2015 | 26395076 |
| dengue: etiology of acute febrile illness in abidjan, côte d'ivoire, in 2011-2012. | the burden of dengue in africa is not well understood. a prospective study was conducted in abidjan, côte d'ivoire from december 2011 to december 2012 to estimate the proportion of dengue and malaria cases among febrile patients during a period when dengue was not known to be circulating in the region, and to describe the clinical and virological characteristics of laboratory-diagnosed dengue cases. | 2015 | 26385938 |
| inferring protective cd8+ t-cell epitopes for ns5 protein of four serotypes of dengue virus chinese isolates based on hla-a, -b and -c allelic distribution: implications for epitope-based universal vaccine design. | dengue is one of the most globally serious vector-borne infectious diseases in tropical and subtropical areas for which there are currently no effective vaccines. the most highly conserved flavivirus protein, ns5, is an indispensable target of cd8+ t-cells, making it an ideal vaccine design target. using the immune epitope database (iedb), cd8+ t-cell epitopes of the dengue virus (denv) ns5 protein were predicted by genotypic frequency of the hla-a,-b, and-c alleles in chinese population. antige ... | 2015 | 26381649 |
| use of centrifugal filter devices to concentrate dengue virus in mosquito per os infection experiments. | dengue virus (denv) is an arbovirus transmitted to humans by the bite of infected aedes mosquitoes. experimental per os infection of mosquitoes with denv is usually a preliminary step in virus/vector studies but it requires being able to prepare artificial blood-meals with high virus titers. we report here the convenient use of centrifugal filter devices to quickly concentrate denv particles in cell-culture supernatants. the median viral titer in concentrated-supernatants was 8.50 log10 tcid50/m ... | 2015 | 26372347 |
| identification of essential containers for aedes larval breeding to control dengue in dhaka, bangladesh. | dengue fever (df), one of the most important emerging arboviral diseases, is transmitted through the bite of container breeding mosquitoes aedes aegypti and aedes albopictus. a household entomological survey was conducted in dhaka from august through october 2000 to inspect water-holding containers in indoor, outdoor, and rooftop locations for aedes larvae. the objective of this study was to determine mosquito productivity of each container type and to identify some risk factors of households in ... | 2015 | 26865829 |
| impact of human mobility on the emergence of dengue epidemics in pakistan. | the recent emergence of dengue viruses into new susceptible human populations throughout asia and the middle east, driven in part by human travel on both local and global scales, represents a significant global health risk, particularly in areas with changing climatic suitability for the mosquito vector. in pakistan, dengue has been endemic for decades in the southern port city of karachi, but large epidemics in the northeast have emerged only since 2011. pakistan is therefore representative of ... | 2015 | 26351662 |
| epidemiological scenario of dengue in brazil. | dengue is the most important reemerging mosquito-borne viral disease worldwide. it is caused by any of four dengue virus types or serotypes (denv-1 to denv-4) and is transmitted by mosquitoes from the genus aedes. ecological changes have favored the geographic expansion of the vector and, since the dengue pandemic in the asian and pacific regions, the infection became widely distributed worldwide, reaching brazil in 1845. the incidence of dengue in brazil has been frequently high, and the number ... | 2015 | 26413514 |
| source and purity of dengue-viral preparations impact requirement for enhancing antibody to induce elevated il-1β secretion: a primary human monocyte model. | dengue virus is a major global health threat and can lead to life-threatening hemorrhagic complications due to immune activation and cytokine production. cross-reactive antibodies to an earlier dengue virus infection are a recognized risk factor for severe disease. these antibodies bind heterologous dengue serotypes and enhance infection into fc-receptor-bearing cells, a process known as antibody-dependent enhancement of infection. one crucial cytokine seen elevated in severe dengue patients is ... | 2015 | 26301593 |
| regulation of gene expression patterns in mosquito reproduction. | in multicellular organisms, development, growth and reproduction require coordinated expression of numerous functional and regulatory genes. insects, in addition to being the most speciose animal group with enormous biological and economical significance, represent outstanding model organisms for studying regulation of synchronized gene expression due to their rapid development and reproduction. disease-transmitting female mosquitoes have adapted uniquely for ingestion and utilization of the hug ... | 2015 | 26274815 |
| expression of dengue virus ns3 protein in drosophila alters its susceptibility to infection. | we developed a drosophila model in which the dengue virus ns3 protein is expressed in a tissue specific and inducible manner. dengue virus ns3 is a multifunctional protein playing a major role during viral replication. both protease and helicase domains of ns3 are interacting with human and insect host proteins including innate immune components of the host machinery. we characterized the ns3 transgenic flies showing that ns3 expression did not affect fly development. to further study the links ... | 2015 | 26267447 |
| cross-sectional community-based study of the socio-demographic factors associated with the prevalence of dengue in the eastern part of sudan in 2011. | dengue is caused by an arthropod-borne flavivirus. infection can be either primary or secondary based on serology, with each stage of the disease characterized by specific serological conversion and antibody formation. further study is needed to fully identify the factors associated with and predisposing to dengue infection. the objective of this study was to identify socio-demographic factors associated with the prevalence of dengue serotypes in kassala state in the eastern part of sudan in 201 ... | 2015 | 26084275 |
| activation of tlr2 and tlr6 by dengue ns1 protein and its implications in the immunopathogenesis of dengue virus infection. | dengue virus (dv) infection is the most prevalent mosquito-borne viral disease and its manifestation has been shown to be contributed in part by the host immune responses. in this study, pathogen recognition receptors, toll-like receptor (tlr) 2 and tlr6 were found to be up-regulated in dv-infected human pbmc using immunofluorescence staining, flow cytometry and western blot analyses. using elisa, il-6 and tnf-α, cytokines downstream of tlr2 and tlr6 signaling pathways were also found to be up-r ... | 2015 | 26226614 |
| characterization of n-glycan structures on the surface of mature dengue 2 virus derived from insect cells. | denv envelope glycoprotein (e) is responsible for interacting with host cell receptors and is the main target for the development of a dengue vaccine based on an induction of neutralizing antibodies. it is well known that denv e glycoprotein has two potential n-linked glycosylation sites at asn67 and asn153. the n-glycans of e glycoprotein have been shown to influence the proper folding of the protein, its cellular localization, its interactions with receptors and its immunogenicity. however, th ... | 2015 | 26208004 |
| interaction of mean temperature and daily fluctuation influences dengue incidence in dhaka, bangladesh. | local weather influences the transmission of the dengue virus. most studies analyzing the relationship between dengue and climate are based on relatively coarse aggregate measures such as mean temperature. here, we include both mean temperature and daily fluctuations in temperature in modelling dengue transmission in dhaka, the capital of bangladesh. we used a negative binomial generalized linear model, adjusted for rainfall, anomalies in sea surface temperature (an index for el niño-southern os ... | 2015 | 26161895 |
| evidence based community mobilization for dengue prevention in nicaragua and mexico (camino verde, the green way): cluster randomized controlled trial. | to test whether community mobilization adds effectiveness to conventional dengue control. | 2015 | 26156323 |
| the global compendium of aedes aegypti and ae. albopictus occurrence. | aedes aegypti and ae. albopictus are the main vectors transmitting dengue and chikungunya viruses. despite being pathogens of global public health importance, knowledge of their vectors' global distribution remains patchy and sparse. a global geographic database of known occurrences of ae. aegypti and ae. albopictus between 1960 and 2014 was compiled. herein we present the database, which comprises occurrence data linked to point or polygon locations, derived from peer-reviewed literature and un ... | 2015 | 26175912 |
| targeted release and fractionation reveal glucuronylated and sulphated n- and o-glycans in larvae of dipteran insects. | mosquitoes are important vectors of parasitic and viral diseases with anopheles gambiae transmitting malaria and aedes aegypti spreading yellow and dengue fevers. using two different approaches (solid-phase extraction and reversed-phase or hydrophilic interaction hplc fractionation followed by maldi-tof ms or permethylation followed by nsi-ms), we examined the n-glycans of both a. gambiae and a. aegypti larvae and demonstrate the presence of a range of paucimannosidic glycans as well as bi- and ... | 2015 | 26047717 |
| reproduction-immunity trade-offs in insects. | immune defense and reproduction are physiologically and energetically demanding processes and have been observed to trade off in a diversity of female insects. increased reproductive effort results in reduced immunity, and reciprocally, infection and activation of the immune system reduce reproductive output. this trade-off can manifest at the physiological level (within an individual) and at the evolutionary level (genetic distinction among individuals in a population). the resource allocation ... | 2015 | 26667271 |
| comparative transcriptomic analysis of immune responses of the migratory locust, locusta migratoria, to challenge by the fungal insect pathogen, metarhizium acridum. | the migratory locust, locusta migratoria manilensis, is an immensely destructive agricultural pest that forms a devastating and voracious gregarious phase. the fungal insect pathogen, metarhizium acridum, is a specialized locust pathogen that has been used as a potent mycoinsecticide for locust control. little, however, is known about locust immune tissue, i.e. fat body and hemocyte, responses to challenge by this fungus. | 2015 | 26503342 |
| transcriptomic analysis of musca domestica to reveal key genes of the prophenoloxidase-activating system. | the propo system regulates melanization in arthropods. however, the genes that are involved in the propo system in housefly musca domestica remain unclear. thus, this study analyzed the combined transcriptome obtained from m. domestica larvae, pupae, and adults that were either normal or bacteria-challenged by an escherichia coli and staphylococcus aureus mixture. a total of 54,821,138 clean reads (4.93 gb) were yielded by illumina sequencing, which were de novo assembled into 89,842 unigenes. o ... | 2015 | 26156588 |
| overexpression of tyrosine hydroxylase and dopa decarboxylase associated with pupal melanization in spodoptera exigua. | melanism has been found in a wide range of species, but the molecular mechanisms involved remain largely elusive. in this study, we studied the molecular mechanisms of the pupal melanism in spodoptera exigua. the full length cdna sequences of tyrosine hydroxylase (th) and dopa decarboxylase (ddc), two key enzymes in the biosynthesis pathway of melanin, were cloned, and their temporal expression patterns in the integument were compared during the larval-pupal metamorphosis process of the s. exigu ... | 2015 | 26084938 |
| key enzymes and proteins of crop insects as candidate for rnai based gene silencing. | rna interference (rnai) is a mechanism of homology dependent gene silencing present in plants and animals. it operates through 21-24 nucleotides small rnas which are processed through a set of core enzymatic machinery that involves dicer and argonaute proteins. in recent past, the technology has been well appreciated toward the control of plant pathogens and insects through suppression of key genes/proteins of infecting organisms. the genes encoding key enzymes/proteins with the great potential ... | 2015 | 25954206 |
| exome and transcriptome sequencing of aedes aegypti identifies a locus that confers resistance to brugia malayi and alters the immune response. | many mosquito species are naturally polymorphic for their abilities to transmit parasites, a feature which is of great interest for controlling vector-borne disease. aedes aegypti, the primary vector of dengue and yellow fever and a laboratory model for studying lymphatic filariasis, is genetically variable for its capacity to harbor the filarial nematode brugia malayi. the genome of ae. aegypti is large and repetitive, making genome resequencing difficult and expensive. we designed exome captur ... | 2015 | 25815506 |
| fipronil and permethrin combination: a novel ectoparasiticide for dogs. | 2015 | 25622552 | |
| an overview of malaria transmission from the perspective of amazon anopheles vectors. | in the americas, areas with a high risk of malaria transmission are mainly located in the amazon forest, which extends across nine countries. one keystone step to understanding the plasmodium life cycle in anopheles species from the amazon region is to obtain experimentally infected mosquito vectors. several attempts to colonise anopheles species have been conducted, but with only short-lived success or no success at all. in this review, we review the literature on malaria transmission from the ... | 2015 | 25742262 |
| evaluation of a protocol for remote identification of mosquito vector species reveals bg-sentinel trap as an efficient tool for anopheles gambiae outdoor collection in burkina faso. | feasibility and costs of monitoring efforts aimed to monitor mosquito species are strictly dependent on the presence of skilled entomologists directly in the field. however, in several contexts this is not possible or easy to organize, thus limiting the possibility to obtain crucial information on presence/abundance of potential disease vectors and of new invasive species. digital imaging approaches could be extremely useful in the frame of medical entomology to overcome this limit. this work de ... | 2015 | 25888896 |
| rotational symmetry of two pyrethroid receptor sites in the mosquito sodium channel. | voltage-gated sodium channels are the primary target of pyrethroid insecticides. although it is well known that specific mutations in insect sodium channels confer knockdown resistance (kdr) to pyrethroids, the atomic mechanisms of pyrethroid-sodium channel interactions are not clearly understood. previously, computer modeling and mutational analysis predicted two pyrethroid receptors, pyrethroid receptor site 1 (pyr1) (initial) and pyrethroid receptor site 2 (pyr2), located in the domain interf ... | 2015 | 25972447 |
| unraveling dual feeding associated molecular complexity of salivary glands in the mosquito anopheles culicifacies. | mosquito salivary glands are well known to facilitate meal acquisition, however the fundamental question on how adult female salivary gland manages molecular responses during sugar versus blood meal uptake remains unanswered. to investigate these responses, we analyzed a total of 58.5 million raw reads generated from two independent rnaseq libraries of the salivary glands collected from 3-4 day-old sugar and blood fed anopheles culicifacies mosquitoes. comprehensive functional annotation analysi ... | 2015 | 26163527 |
| small-scale evaluation of the efficacy and residual activity of alpha-cypermethrin wg (250 g ai/kg) for indoor spraying in comparison with alpha-cypermethrin wp (50 g ai/kg) in india. | indoor residual spraying (irs) with different formulations of insecticides is being used for the control of mosquito vectors in many countries. in the present study, residual efficacy and duration of effectiveness of irs with alpha-cypermethrin wg-sb (250 g ai/m2) formulation was compared with wp formulation (50 g ai/kg) in a small scale (phase ii) field trial. | 2015 | 26022085 |
| diversification of the genus anopheles and a neotropical clade from the late cretaceous. | the anopheles genus is a member of the culicidae family and consists of approximately 460 recognized species. the genus is composed of 7 subgenera with diverse geographical distributions. despite its huge medical importance, a consensus has not been reached on the phylogenetic relationships among anopheles subgenera. we assembled a comprehensive dataset comprising the coi, coii and 5.8s rrna genes and used maximum likelihood and bayesian inference to estimate the phylogeny and divergence times o ... | 2015 | 26244561 |