Publications

the descriptive epidemiology of yersiniosis: a multistate study, 2005-2011.	yersiniosis, a foodborne infection of zoonotic origin caused by the bacteria yersinia enterocolitica and yersinia pseudotuberculosis, is a reportable disease in 38 states. both sporadic and foodborne outbreaks of yersiniosis have been reported in the u.s., with annual occurrence of an estimated 98,000 episodes of illness, 533 hospitalizations, and 29 deaths. we analyzed surveillance data from nine non-foodnet-participating u.s. states during the period 2005-2011 to describe the epidemiology of t ...	2017	25931631
x-ray crystal structures of the pheromone-binding domains of two quorum-hindered transcription factors, yenr of yersinia enterocolitica and cepr2 of burkholderia cenocepacia.	the ability of luxr-type proteins to regulate transcription is controlled by bacterial pheromones, n-acylhomoserine lactones (ahls). most luxr-family proteins require their cognate ahls for activity, and some of them require ahls for folding and stability, and for protease-resistance. however, a few members of this family are able to fold, dimerize, bind dna, and regulate transcription in the absence of ahls; moreover, these proteins are antagonized by their cognate ahls. one such protein is yen ...	2017	28614901
stand-alone eal domain proteins form a distinct subclass of eal proteins involved in regulation of cell motility and biofilm formation in enterobacteria.	the second messenger cyclic dimeric gmp (c-di-gmp) is almost ubiquitous among bacteria as are the c-di-gmp turnover proteins, which mediate the transition between motility and sessility. eal domain proteins have been characterized as c-di-gmp-specific phosphodiesterases. while most eal domain proteins contain additional, usually n-terminal, domains, there is a distinct family of proteins with stand-alone eal domains, exemplified by salmonella enterica serovar typhimurium proteins stm3611 (yhjh/p ...	2017	28652301
endogenous hepcidin and its agonist mediate resistance to selected infections by clearing non-transferrin-bound iron.	the iron-regulatory hormone hepcidin is induced early in infection, causing iron sequestration in macrophages and decreased plasma iron; this is proposed to limit the replication of extracellular microbes, but could also promote infection with macrophage-tropic pathogens. the mechanisms by which hepcidin and hypoferremia modulate host defense, and the spectrum of microbes affected, are poorly understood. using mouse models, we show that hepcidin was selectively protective against siderophilic ex ...	2017	28465342
evaluation of virulence genes in yersinia enterocolitica strains using sybr green real-time pcr.	yersinia enterocolitica comprises six biotypes 1a, 1b, 2, 3, 4, and 5. the virulence of the strains belonging to biotypes 1b and 2-5 depends on the presence of both chromosomal and plasmid-borne genes. strains belonging to biotype 1a do not carry the virulence plasmid pyv. however, they carry other virulence genes, such as ystb and hrep. the aim of this study was to investigate the distribution of yada, virf, inv, ysta, ystb, myfa, hrep and ymoa virulence genes in y. enterocolitica strains in or ...	2017	28400007
galectin-1-driven tolerogenic programs aggravate yersinia enterocolitica infection by repressing antibacterial immunity.	yersinia enterocolitica is an enteropathogenic bacterium that causes gastrointestinal disorders, as well as extraintestinal manifestations. to subvert the host's immune response, y. enterocolitica uses a type iii secretion system consisting of an injectisome and effector proteins, called yersinia outer proteins (yops), that modulate activation, signaling, and survival of immune cells. in this article, we show that galectin-1 (gal-1), an immunoregulatory lectin widely expressed in mucosal tissues ...	2017	28716827
secondary focal form of yersinia enterocolitica infection with prolonged polyarthritis in young caucasian male: a case report.	current issue deals with an interesting clinical case of a rare infectious disease in a caucasian young male patient, caused by yersinia enterocоlitica. infection proceeded in the development of secondary focal form, which was accompanied by prolonged polyarthritis. we described a clinical case of secondary focal form with prolonged polyarthritis caused by y. enterocolitica o:3 serogroup in young patient with the purpose of focusing on the early clinical and laboratory diagnosistics of yersinios ...	2017	28711901
prevalence of yersinia enterocolitica bioserotype 3/o:3 among children with diarrhea, china, 2010-2015.	yersinia enterocolitica is thought to not significantly contribute to diarrheal disease in china, but evidence substantiating this claim is limited. we determined the prevalence of y. enterocolitica infection and strain types present among children <5 years of age with diarrhea in china. the overall prevalence of pathogenic isolates was 0.59%. prevalence of pathogenic bioserotype 3/o:3 varied geographically. in this population, the presence of fecal leukocytes was a characteristic of y. enteroco ...	2017	28820132
draft genome sequences of yersinia frederiksenii, yersinia intermedia, and yersinia kristensenii strains from brazil.	yersinia enterocolitica-like strains are usually understudied. in this work, we reported the draft genome sequences of two yersinia frederiksenii, two yersinia intermedia, and two yersinia kristensenii strains isolated from humans, animals, food, and the environment in brazil. these draft genomes will provide better molecular characterizations of these species.	2017	28798182
engineering of yersinia phytases to improve pepsin and trypsin resistance and thermostability and application potential in the food and feed industry.	susceptibility to proteases usually limits the application of phytase. we sought to improve the pepsin and trypsin resistance of yeappa from yersinia enterocolitica and ykappa from y. kristensenii by optimizing amino acid polarity and charge. the predicted pepsin/trypsin cleavage sites f89/k226 in pepsin/trypsin-sensitive yeappa and the corresponding sites (f89/e226) in pepsin-sensitive but trypsin-resistant ykappa were substituted with s and h, respectively. six variants were produced in pichia ...	2017	28752758
low prevalence of human enteropathogenic yersinia spp. in brown rats (rattus norvegicus) in flanders.	brown rats (rattus norvegicus) have been identified as potential carriers of yersinia enterocolitica and y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in europe. enteropathogenic yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. information is however lacking regarding the extent to which rats act as carriers of these yersinia spp.. in 2013, ...	2017	28403184
development of a novel multiplexed qpcr and pyrosequencing method for the detection of human pathogenic yersiniae.	the purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. yersinia enterocolitica, y. pseudotuberculosis and y. pestis) in complex food samples. the assay combines multiplexed real-time pcr (qpcr) and pyrosequencing for detecting and differentiating human pathogenic yersiniae with high confidence through sequence based confirmation. the assay demonstrated 100% specificity and inclusivity when tested against a panel of 14 y. ...	2017	28704728
a dynamic and adaptive network of cytosolic interactions governs protein export by the t3ss injectisome.	many bacteria use a type iii secretion system (t3ss) to inject effector proteins into host cells. selection and export of the effectors is controlled by a set of soluble proteins at the cytosolic interface of the membrane spanning type iii secretion 'injectisome'. combining fluorescence microscopy, biochemical interaction studies and fluorescence correlation spectroscopy, we show that in live yersinia enterocolitica bacteria these soluble proteins form complexes both at the injectisome and in th ...	2017	28653671
reactive arthritis caused by yersinia enterocolitica enteritis.	we report a case of reactive arthritis (rea) triggered by yersinia enterocolitica enteritis. a 24-year-old japanese man developed polyarthritis in the lower limbs. two weeks prior to these symptoms, he noted diarrhea, right lower abdominal pain and a fever. y. enterocolitica was not isolated from a stool culture; however, he was diagnosed with rea based on the colonoscopic findings of a high anti-y. enterocolitica antibody titer and hla-b27 antigen positivity. following treatment with methotrexa ...	2017	28502944
an improved kinetic assay for the characterization of metal-dependent pectate lyases.	pectate lyases are a subset of polysaccharide lyases (pls) that specifically utilize a metal dependent β-elimination mechanism to cleave glyosidic bonds in homogalacturonan (hg; α-d-1,4-galacturonic acid). most commonly, pls harness calcium for catalysis; however, some pl families (e.g., pl2 and pl22) display preferences for transitional metals. deploying alternative metals during β-elimination is correlated with signature coordination pocket chemistry, and is reflective of the evolution, functi ...	2017	28417359
draft genome sequences of four yersinia enterocolitica strains, isolated from wild ungulate carcasses.	this study describes the draft genome sequences of four yersinia enterocolitica strains, originally isolated from ungulate carcasses. these isolates were typed biochemically and two were determined to be highly virulent (biotype 1b). the draft genome sequences had a mean size of 4.77 mb and a mean g+c content of 47.1%.	2017	28408689
lactoferrin protects against intestinal inflammation and bacteria-induced barrier dysfunction in vitro.	the iron-binding glycoprotein lactoferrin (lf) is naturally present in human breast milk. several studies suggest that lf contributes to infant health and development owing to a variety of protective effects, including antimicrobial and anti-inflammatory features. therefore, we aimed to elucidate its protective properties on intestinal epithelial barrier dysfunction induced by infection or inflammation using the human epithelial cell culture models ht-29/b6 and t84. during barrier perturbation i ...	2017	28614589
thirty years of human infections caused by yersinia enterocolitica in northern spain: 1985-2014.	yersinia enterocolitica infection is a zoonosis with worldwide distribution, gastroenteritis being by far the most common clinical manifestation of human infection. in gipuzkoa, northern spain, human y. enterocolitica infections increased from the mid-1980s to the beginning of the 21st century (from 7·9 to 23·2 annual episodes per 100 000 population) to decrease to 7·2 annual episodes per 100 000 population in the last years of the study. the hospital admission rate due to yersiniosis during the ...	2017	28578732
next-generation sequencing virulome analysis of a yersinia enterocolitica subsp. palearctica bioserotype 4/o:3 st18 isolated from human blood in brazil.	yersinia enterocolitica is a widespread gram-negative bacterium that causes gastrointestinal disease and other clinical manifestations in humans. potentially pathogenic y. enterocolitica has been isolated in brazil, from human, environmental, food, and animal sources. herein we report a genome sequence of y. enterocolitica subsp. palearctica strain ye 19, serotype o:3, biotype 4, sequence type 18, with virulence determinants isolated from human blood in rio de janeiro in 2005. the results corrob ...	2017	28571687