Publications

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development of a novel multiplexed qpcr and pyrosequencing method for the detection of human pathogenic yersiniae.the purpose of this study was to develop a novel and robust molecular assay for the detection of human pathogenic yersiniae (i.e. yersinia enterocolitica, y. pseudotuberculosis and y. pestis) in complex food samples. the assay combines multiplexed real-time pcr (qpcr) and pyrosequencing for detecting and differentiating human pathogenic yersiniae with high confidence through sequence based confirmation. the assay demonstrated 100% specificity and inclusivity when tested against a panel of 14 y. ...201728704728
a dynamic and adaptive network of cytosolic interactions governs protein export by the t3ss injectisome.many bacteria use a type iii secretion system (t3ss) to inject effector proteins into host cells. selection and export of the effectors is controlled by a set of soluble proteins at the cytosolic interface of the membrane spanning type iii secretion 'injectisome'. combining fluorescence microscopy, biochemical interaction studies and fluorescence correlation spectroscopy, we show that in live yersinia enterocolitica bacteria these soluble proteins form complexes both at the injectisome and in th ...201728653671
lactoferrin protects against intestinal inflammation and bacteria-induced barrier dysfunction in vitro.the iron-binding glycoprotein lactoferrin (lf) is naturally present in human breast milk. several studies suggest that lf contributes to infant health and development owing to a variety of protective effects, including antimicrobial and anti-inflammatory features. therefore, we aimed to elucidate its protective properties on intestinal epithelial barrier dysfunction induced by infection or inflammation using the human epithelial cell culture models ht-29/b6 and t84. during barrier perturbation i ...201728614589
thirty years of human infections caused by yersinia enterocolitica in northern spain: 1985-2014.yersinia enterocolitica infection is a zoonosis with worldwide distribution, gastroenteritis being by far the most common clinical manifestation of human infection. in gipuzkoa, northern spain, human y. enterocolitica infections increased from the mid-1980s to the beginning of the 21st century (from 7·9 to 23·2 annual episodes per 100 000 population) to decrease to 7·2 annual episodes per 100 000 population in the last years of the study. the hospital admission rate due to yersiniosis during the ...201728578732
next-generation sequencing virulome analysis of a yersinia enterocolitica subsp. palearctica bioserotype 4/o:3 st18 isolated from human blood in brazil.yersinia enterocolitica is a widespread gram-negative bacterium that causes gastrointestinal disease and other clinical manifestations in humans. potentially pathogenic y. enterocolitica has been isolated in brazil, from human, environmental, food, and animal sources. herein we report a genome sequence of y. enterocolitica subsp. palearctica strain ye 19, serotype o:3, biotype 4, sequence type 18, with virulence determinants isolated from human blood in rio de janeiro in 2005. the results corrob ...201728571687
antimicrobial resistance and plasmid replicons in yersinia enterocolitica strains isolated in brazil in 30 years.some studies evaluated the resistance profile of the y. enterocolitica strains isolated in diverse countries. however, in brazil the isolation and the study of y. enterocolitica are not common and therefore information about the antimicrobial resistance profile of this species in this country is scarce. therefore, the aim of this study was to evaluate the antimicrobial resistance of y. enterocolitica of biotypes 1a, 2 and 4 isolated from clinical and non-clinical sources between 1979 and 2012, i ...201728558260
development of an lps-based elisa for diagnosis of yersinia enterocolitica o:3 infections in danish patients: a follow-up study.the bacterium yersinia enterocolitica causes gastroenteritis in humans. the study aimed to develop a diagnostic enzyme-linked immunosorbent assay (elisa) for detection of yersinia enterocolitica o:3 lps antibodies in sera from danish patients with suspected yersinia enterocolitica o:3 gastrointestinal infection. as a part of this, antibody decay profiles after culture confirmed yersinia enteritis were studied.201728545413
genetic diversity, virulotyping and antimicrobial resistance susceptibility of yersinia enterocolitica isolated from pigs and porcine products in malaysia.the objectives of the present study were to determine the antimicrobial resistance, virulotypes and genetic diversity of yersinia enterocolitica isolated from uncooked porcine food and live pigs in malaysia.201728542807
comparison of growth and the cytokines induced by pathogenic yersinia enterocolitica bio-serotypes 3/o: 3 and 2/o: 9.pathogenic yersinia enterocolitica is widely distributed in china where the primary bio-serotypes are 3/o: 3 and 2/o: 9. recently, the distribution of 2/o: 9 strains are being gradually replaced by 3/o: 3 strains where presently 3/o: 3 strains are the major pathogenic y. enterocolitica in china. to identify the growth conditions and cytokines induced by y. enterocolitica and providing some clues for this shift, we performed competitive growth in vitro and in vivo for these two bio-serotype strai ...201728507952
reactive arthritis caused by yersinia enterocolitica enteritis.we report a case of reactive arthritis (rea) triggered by yersinia enterocolitica enteritis. a 24-year-old japanese man developed polyarthritis in the lower limbs. two weeks prior to these symptoms, he noted diarrhea, right lower abdominal pain and a fever. y. enterocolitica was not isolated from a stool culture; however, he was diagnosed with rea based on the colonoscopic findings of a high anti-y. enterocolitica antibody titer and hla-b27 antigen positivity. following treatment with methotrexa ...201728502944
an improved kinetic assay for the characterization of metal-dependent pectate lyases.pectate lyases are a subset of polysaccharide lyases (pls) that specifically utilize a metal dependent β-elimination mechanism to cleave glyosidic bonds in homogalacturonan (hg; α-d-1,4-galacturonic acid). most commonly, pls harness calcium for catalysis; however, some pl families (e.g., pl2 and pl22) display preferences for transitional metals. deploying alternative metals during β-elimination is correlated with signature coordination pocket chemistry, and is reflective of the evolution, functi ...201728417359
draft genome sequences of four yersinia enterocolitica strains, isolated from wild ungulate carcasses.this study describes the draft genome sequences of four yersinia enterocolitica strains, originally isolated from ungulate carcasses. these isolates were typed biochemically and two were determined to be highly virulent (biotype 1b). the draft genome sequences had a mean size of 4.77 mb and a mean g+c content of 47.1%.201728408689
low prevalence of human enteropathogenic yersinia spp. in brown rats (rattus norvegicus) in flanders.brown rats (rattus norvegicus) have been identified as potential carriers of yersinia enterocolitica and y. pseudotuberculosis, the etiological agents of yersiniosis, the third most reported bacterial zoonosis in europe. enteropathogenic yersinia spp. are most often isolated from rats during yersiniosis cases in animals and humans, and from rats inhabiting farms and slaughterhouses. information is however lacking regarding the extent to which rats act as carriers of these yersinia spp.. in 2013, ...201728403184
evaluation of virulence genes in yersinia enterocolitica strains using sybr green real-time pcr.yersinia enterocolitica comprises six biotypes 1a, 1b, 2, 3, 4, and 5. the virulence of the strains belonging to biotypes 1b and 2-5 depends on the presence of both chromosomal and plasmid-borne genes. strains belonging to biotype 1a do not carry the virulence plasmid pyv. however, they carry other virulence genes, such as ystb and hrep. the aim of this study was to investigate the distribution of yada, virf, inv, ysta, ystb, myfa, hrep and ymoa virulence genes in y. enterocolitica strains in or ...201728400007
impact of acanthamoeba cysts on stress resistance of salmonella enterica serovar typhimurium, yersinia enterocolitica 4/o:3, listeria monocytogenes 1/2a, and escherichia coli o:26.the formation of robust resting cysts enables acanthamoeba to resist harsh environmental conditions. this study investigated to what extent these cysts are resistant to physical and chemical stresses as applied in food industry cleaning and disinfection procedures. moreover, it was assessed whether certain intracystic meat-borne bacterial pathogens are more stress resistant than free-living bacterial monocultures and if intracystic passage and subsequent association with trophozoites induces cro ...201728526786
green catanionic gemini surfactant-lichenysin mixture: improved surface, antimicrobial, and physiological properties.catanionic surfactant mixtures form a wide variety of organized assemblies and aggregates with improved physicochemical and biological properties. the green catanionic mixture n(α)n(ω)-bis(n(α)caproylarginine) α,ω-propyldiamide (c3(ca)2):lichenysin (molar ratio 8:2) showed antimicrobial synergies against yersinia enterocolitica, bacillus subtilis, escherichia coli o157:h7, and candida albicans. flow cytometry and viability studies indicated that this catanionic mixture increases the probability ...201728636319
investigation into the antimicrobial action and mechanism of a novel endogenous peptide β-casein 197 from human milk.a novel endogenous peptide cleaved from 197-213 aa of β-casein, named β-casein 197, was identified by tandem mass spectrometry. β-casein 197 constituted a significant proportion of the peptide content in preterm milk. this study investigated the antibacterial effects and mechanisms against common pathogenic bacteria. six bacterial strains were selected for this study: escherichia coli, staphylococcus aureus, yersinia enterocolitica, listeria monocytogenes, klebsiella pneumonia and bacillus subti ...201728591979
emerging microbiota during cold storage and temperature abuse of ready-to-eat salad​.introduction: ready-to-eat (rte) leafy vegetables have a natural leaf microbiota that changes during different processing and handling steps from farm to fork. the objectives of this study were (i) to compare the microbiota of rte baby spinach and mixed-ingredient salad before and after seven days of storage at 8°c or 15°c; (ii) to explore associations between bacterial communities and the foodborne pathogens listeria monocytogenes, pathogenic yersinia enterocolitica, and pathogen model organism ...201728649305
characterization of high exopolysaccharide-producing lactobacillus strains isolated from mustard pickles for potential probiotic applications.the aim of this study was to characterize high exopolysaccharide (eps)-producing lactic acid bacteria (lab) isolated from mustard pickles in taiwan for potential probiotic applications. among 39 collected lab strains, four most productive eps-producing strains were selected for further analysis. comparative analyses of 16s rdna genes rpoa and phes sequences demonstrated that these strains were members of lactobacillus plantarum-group (lpg). ncd 2, nld 4, slc 13, and nld 16 showed survival rates ...201728617525
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