Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| assessment of toxicity and biodegradability on activated sludge of priority and emerging pollutants. | several methods for evaluating the toxicity and biodegradability of hazardous pollutants (chlorinated compounds, chemical additives and pharmaceuticals) have been studied in this work. different bioassays using representative bacteria of marine and terrestrial ecosystems such as vibrio fischeri and pseudomonas putida have been used to assess the ecotoxicity. activated sludge was used to analyse the effect of those pollutants in a biological reactor of a sewage treatment plant (stp). the results ... | 2016 | 26243262 |
| a newly isolated pseudomonas putida s-1 strain for batch-mode-propanethiol degradation and continuous treatment of propanethiol-containing waste gas. | pseudomonas putida s-1 was isolated from activated sludge. this novel strain was capable of degrading malodorous 1-propanethiol (pt). pt degradation commenced with no lag phase by cells pre-grown in nutrition-rich media, such as luria-bertani (lb), and pt-contained mineral medium at specific growth rates of 0.10-0.19 h(-1); this phenomenon indicated the operability of a large-scale cell culture. a possible pt degradation pathway was proposed on the basis of the detected metabolites, including di ... | 2016 | 26476310 |
| a holistic view of polyhydroxyalkanoate metabolism in pseudomonas putida. | polyhydroxyalkanoate (pha) metabolism has been traditionally considered as a futile cycle involved in carbon and energy storage. the use of cutting-edge technologies linked to systems biology has improved our understanding of the interaction between bacterial physiology, pha metabolism and other cell functions in model bacteria such as pseudomonas putida kt2440. pha granules or carbonosomes are supramolecular complexes of biopolyester and proteins that are essential for granule segregation durin ... | 2016 | 25556983 |
| influence of the crc regulator on the hierarchical use of carbon sources from a complete medium in pseudomonas. | the crc protein, together with the hfq protein, participates in catabolite repression in pseudomonads, helping to coordinate metabolism. little is known about how crc affects the hierarchy of metabolite assimilation from complex mixtures. using proton nuclear magnetic resonance (nmr) spectroscopy, we carried out comprehensive metabolite profiling of culture supernatants (metabolic footprinting) over the course of growth of both pseudomonas putida and p. aeruginosa, and compared the wild-type str ... | 2016 | 26568055 |
| genetically engineered pseudomonas putida x3 strain and its potential ability to bioremediate soil microcosms contaminated with methyl parathion and cadmium. | a multifunctional pseudomonas putida x3 strain was successfully engineered by introducing methyl parathion (mp)-degrading gene and enhanced green fluorescent protein (egfp) gene in p. putida x4 (cctcc: 209319). in liquid cultures, the engineered x3 strain utilized mp as sole carbon source for growth and degraded 100 mg l(-1) of mp within 24 h; however, this strain did not further metabolize p-nitrophenol (pnp), an intermediate metabolite of mp. no discrepancy in minimum inhibitory concentrations ... | 2016 | 26521245 |
| environment-induced conformational and functional changes of l-2-haloacid dehalogenase. | 2-haloacid dehalogenases have been highly studied due to their potential applications in chemical industries and bioremediation. although biochemical and structural characterizations of the enzyme have been detailed, no information was available regarding environmental effects on the structure-function relationship. here, circular dichroism spectroscopy (cd) was used to investigate the correlation between changes on the conformation and the function of l-2-haloacid dehalogenase (hadl aj1) from t ... | 2016 | 26508534 |
| effects of even and odd number fatty acids cofeeding on pha production and composition in pseudomonas putida bet001 isolated from palm oil mill effluent. | the biosynthesis of medium-chain-length poly-3-hydroxyalkanoates by pseudomonas putida bet001 cultivated on mixed carbon sources was investigated. the mixed carbon sources consisted of heptanoic acid (ha) and oleic acid (oa). a relatively low pha content at 1.2% (w/w) and 11.4% (w/w) was obtained when ha or oa was used as the sole carbon source. when these fatty acids were supplied as a mixture, pha content increased threefold. interestingly, the mixture-derived pha composed of both odd and even ... | 2016 | 25643814 |
| a novel enterocin t1 with anti-pseudomonas activity produced by enterococcus faecium t1 from chinese tibet cheese. | an enterocin-producing enterococcus faecium t1 was isolated from chinese tibet cheese. the enterocin was purified by sp-sepharose and reversed phase hplc. it was identified as unique from other reported bacteriocins based on molecular weight (4629 da) and amino acid compositions; therefore it was subsequently named enterocin t1. enterocin t1 was stable at 80-100 °c and over a wide ph range, ph 3.0-10.0. protease sensitivity was observed to trypsin, pepsin, papain, proteinase k, and pronase e. im ... | 2016 | 26745981 |
| identification and characterization of a novel (+)-γ-lactamase from microbacterium hydrocarbonoxydans. | 2-azabicyclo[2.2.1]hept-5-en-3-one (γ-lactam) is an important precursor of many carbocyclic nucleoside analogs and pharmaceuticals. (-)-γ-lactam has attracted much attention because of its role as an intermediate of antiviral drugs such as abacavir and carbovir. (+)-γ-lactamase can be used for the kinetic resolution of γ-lactam to obtain (-)-γ-lactam. in this study, a novel (+)-γ-lactamase (mh33h4-5540) was discovered from the gene library of microbacterium hydrocarbonoxydans based on a colorime ... | 2016 | 27255489 |
| stereochemical consequences of vinylpyruvate hydratase-catalyzed reactions. | a stereochemical analysis has been carried out on two vinylpyruvate hydratases (vph), which convert 2-hydroxy-2,4-pentadienoate to 2-keto-4s-hydroxypentanoate in meta-fission pathways. bacterial strains with this pathway can use aromatic compounds as sole sources of energy and carbon. the analysis was carried out using the 5-methyl and 5-chloro derivatives of 2-hydroxy-2,4-pentadienoate with the enzymes from pseudomonas putida mt-2 (pp) and leptothrix cholodnii sp-6 (lc). in both organisms, vph ... | 2016 | 27362840 |
| two novel sets of genes essential for nicotine degradation by sphingomonas melonis ty. | nicotine is a type of environmental pollutant present in the tobacco waste that is generated during tobacco manufacturing. sphingomonas melonis ty can utilize nicotine as a sole source of carbon, nitrogen and energy via a variant of the pyridine and pyrrolidine pathway (the vpp pathway). in this study, we report the identification of two novel sets of genes, ndra1a2a3, and ndrb1b2b3b4, which are crucial for nicotine degradation by strain ty. ndra1a2a3 and ndrb1b2b3b4 exhibit similarity with both ... | 2016 | 28144232 |
| nutrition effects on the biofilm immobilization and 3,5-dnba degradation of comamonas testosteroni a3 during bioaugmentation treatment. | the survival of inoculated microbes is critical for successful bioaugmentation in wastewater treatment. the influence of readily available nutrients (rans) on the colonization of two functional bacteria, pseudomonas putida m9, a strong biofilm-forming strain, and comamonas testosteroni a3, a 3,5-dinitrobenzoic acid (3,5-dnba)-degrading strain, in biofilms was studied with 3,5-dinitrobenoic acid synthetic wastewater (dcmm) complemented with various ratios of luria-bertani broth (lb). with the inc ... | 2016 | 25345550 |
| biotechnological production of aromatic compounds of the extended shikimate pathway from renewable biomass. | aromatic chemicals that contain an unsaturated ring with alternating double and single bonds find numerous applications in a wide range of industries, e.g. paper and dye manufacture, as fuel additives, electrical insulation, resins, pharmaceuticals, agrochemicals, in food, feed and cosmetics. their chemical production is based on petroleum (btx; benzene, toluene, and xylene), but they can also be obtained from plants by extraction. due to petroleum depletion, health compliance, or environmental ... | 2016 | 27871872 |
| extraction method plays critical role in antibacterial activity of propolis-loaded hydrogels. | extracted propolis has been used for a long time as a remedy. however, if the release rate of propolis is not controlled, the efficacy is reduced. to overcome this issue, extracted propolis was added to a cryogel system. propolis collected from southern brazil was extracted using different methods and loaded at different concentrations into polyvinyl alcohol (pva) and polyacrylic acid hydrogels as carrier systems. the material properties were investigated with a focus on the propolis release pro ... | 2016 | 26886307 |
| identification of a chemoreceptor that specifically mediates chemotaxis toward metabolizable purine derivatives. | chemotaxis is an essential mechanism that enables bacteria to move toward favorable ecological niches. escherichia coli, the historical model organism for studying chemotaxis, has five well-studied chemoreceptors. however, many bacteria with different lifestyle have more chemoreceptors, most of unknown function. using a high throughput screening approach, we identified a chemoreceptor from pseudomonas putida kt2440, named mcph, which specifically recognizes purine and its derivatives, adenine, g ... | 2016 | 26355499 |
| dna sequence-selective c8-linked pyrrolobenzodiazepine-heterocyclic polyamide conjugates show anti-tubercular-specific activities. | new chemotherapeutic agents with novel mechanisms of action are in urgent need to combat the tuberculosis pandemic. a library of 12 c8-linked pyrrolo[2,1-c][1,4]benzodiazepine (pbd)-heterocyclic polyamide conjugates (1-12) was evaluated for anti-tubercular activity and dna sequence selectivity. the pbd conjugates were screened against slow-growing mycobacterium bovis bacillus calmette-guérin and m. tuberculosis h37rv, and fast-growing escherichia coli, pseudomonas putida and rhodococcus sp. rha1 ... | 2016 | 27168314 |
| integrated acoustic separation, enrichment, and microchip polymerase chain reaction detection of bacteria from blood for rapid sepsis diagnostics. | this paper describes an integrated microsystem for rapid separation, enrichment, and detection of bacteria from blood, addressing the unmet clinical need for rapid sepsis diagnostics. the blood sample is first processed in an acoustophoresis chip, where red blood cells are focused to the center of the channel by an acoustic standing wave and sequentially removed. the bacteria-containing plasma proceeds to a glass capillary with a localized acoustic standing wave field where the bacteria are trap ... | 2016 | 27264110 |
| genetic programming of catalytic pseudomonas putida biofilms for boosting biodegradation of haloalkanes. | bacterial biofilms outperform planktonic counterparts in whole-cell biocatalysis. the transition between planktonic and biofilm lifestyles of the platform strain pseudomonas putida kt2440 is ruled by a regulatory network controlling the levels of the trigger signal cyclic di-gmp (c-di-gmp). this circumstance was exploited for designing a genetic device that over-runs the synthesis or degradation of c-di-gmp--thus making p. putida to form biofilms at user's will. for this purpose, the transcripti ... | 2016 | 26620533 |
| unusual poly-3-hydroxyalkanoates (phas) biosynthesis behavior of pseudomonas putida bet001 and delftia tsuruhatensis bet002 isolated from palm oil mill effluent. | pseudomonas putida bet001 and delftia tsuruhatensis bet002, isolated from palm oil mill effluent, accumulated poly (3-hydroxyalkanoates) (pha) when grown on aliphatic fatty acids, sugars and glycerol. the substrates were supplied at 20:1 c/n mole ratio. among c-even n-alkanoic acids, myristic acid gave the highest pha content 26 and 28 wt% in p. putida and d. tsuruhatensis respectively. among c-odd n-alkanoic acids, undecanoic gave the highest pha content at 40 wt% in p. putida and 46 wt% in d. ... | 2016 | 26800648 |
| investigation of plasmid-induced growth defect in pseudomonas putida. | genetic engineering in bacteria mainly relies on the use of plasmids. but despite their pervasive use for physiological studies as well as for the design and optimization of industrially used production strains, only limited information about plasmid induced growth defects is available for different replicons and organisms. here, we present the identification and characterization of such a phenomenon for pseudomonas putida transformants carrying the pbbr1-derived plasmid pmis1. we identified the ... | 2016 | 27287537 |
| structural similarities and differences in h-ns family proteins revealed by the n-terminal structure of turb in pseudomonas putida kt2440. | h-ns family proteins play key roles in bacterial nucleoid compaction and global transcription. mvat homologues in pseudomonas have almost negligible amino acid sequence identity with h-ns, but can complement an hns-related phenotype of escherichia coli. here, we report the crystal structure of the n-terminal dimerization/oligomerization domain of turb, an mvat homologue in pseudomonas putida kt2440. our data identify two dimerization sites; the structure of the central dimerization site is almos ... | 2016 | 27709616 |
| screening through the plicable promoter toolbox enhances protein production in escherichia coli. | escherichia coli is a common host for recombinant protein production in which production titers are highly dependent on the employed expression system. promoters are thereby a key element to control gene expression levels. in this study, a novel plicable promoter toolbox was developed which enables in a single cloning step and after a screening experiment to identify out of ten iptg-inducible promoters (t7, a3, lpp, tac, pac, sp6, lac, npr, trc and syn) the most suitable one for high level prote ... | 2016 | 27753230 |
| regulation of hydroxylation and nitroreduction pathways during metabolism of the neonicotinoid insecticide imidacloprid by pseudomonas putida. | imidacloprid (imi) is mainly metabolized via nitroreduction and hydroxylation pathways, which produce different metabolites that are toxic to mammals and insects. however, regulation of imi metabolic flux between nitroreduction and hydroxylation pathways is still unclear. in this study, pseudomonas putida was found to metabolize imi to 5-hydroxy and nitroso imi and was therefore used for investigating the regulation of imi metabolic flux. the cell growth time, cosubstrate, dissolved oxygen conce ... | 2016 | 27230024 |
| design of inducible expression vectors for improved protein production in ralstonia eutropha h16 derived host strains. | ralstonia eutropha h16 (cupriavidus necator h16) is a gram-negative, facultative chemolithoautotrophic bacterium which can use h2 and co2 as sole energy and carbon sources in the absence of organic substrates. the biotechnological use of r. eutropha h16 on an industrial scale has already been established; however, only a small number of tools promoting inducible gene expression is available. within this study two systems promoting inducible expression were designed on the basis of the strong j5 ... | 2016 | 27085887 |
| the ssr protein (t1e_1405) from pseudomonas putida dot-t1e enables oligonucleotide-based recombineering in platform strain p. putida em42. | some strains of the soil bacterium pseudomonas putida have become in recent years platforms of choice for hosting biotransformations of industrial interest. despite availability of many genetic tools for this microorganism, genomic editing of the cell factory p. putida em42 (a derivative of reference strain kt2440) is still a time-consuming endeavor. in this work we have investigated the in vivo activity of the ssr protein encoded by the open reading frame t1e_1405 from pseudomonas putida dot-t1 ... | 2016 | 27367544 |
| esterase estk from pseudomonas putida mt-2: an enantioselective acetylesterase with activity for deacetylation of xylan and poly(vinylacetate). | an extracellular esterase gene estk was identified in pseudomonas putida mt-2, and was overexpressed to high levels in escherichia coli. the recombinant estk enzyme was purified and characterised kinetically against p-nitrophenyl ester and other aryl-alkyl ester substrates, and was found to be selective for hydrolysis of acetyl ester substrates, with high activity for p-nitrophenyl acetate (kcat 5.5 s(-1) , km 285 μm). recombinant estk was found to catalyse deacetylation of acetylated beech xyla ... | 2016 | 27696534 |
| the expression of many chemoreceptor genes depends on the cognate chemoeffector as well as on the growth medium and phase. | chemoreceptor-based signaling is a major bacterial signal transduction mechanism. escherichia coli, the traditional model, has five chemoreceptors. recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. pseudomonas putida kt2440 is an alternative model that has 27 chemoreceptors and the cognate chemoeffector is known for many of them. here, we address the question whether and which factors modulate chemoreceptor gene expression. we report reverse transc ... | 2016 | 27632030 |
| nitrile-synthesizing enzyme: gene cloning, overexpression and application for the production of useful compounds. | one of the nitrile-synthesizing enzymes, β-cyano-l-alanine synthase, catalyzes β-cyano-l-alanine (β-cnala) from potassium cyanide and o-acetyl-l-serine or l-cysteine. we have identified this enzyme from pseudomonas ovalis no. 111. in this study, we cloned the β-cnala synthase gene and expressed it in escherichia coli and rhodococcus rhodochrous. furthermore, we carried out co-expression of β-cnala synthase with nitrilase or nitrile hydratases in order to synthesize aspartic acid and asparagine f ... | 2016 | 27250664 |
| cyp101j2, cyp101j3, and cyp101j4, 1,8-cineole-hydroxylating cytochrome p450 monooxygenases from sphingobium yanoikuyae strain b2. | we report the isolation and characterization of three new cytochrome p450 monooxygenases: cyp101j2, cyp101j3, and cyp101j4. these p450s were derived from sphingobium yanoikuyae b2, a strain that was isolated from activated sludge based on its ability to fully mineralize 1,8-cineole. genome sequencing of this strain in combination with purification of native 1,8-cineole-binding proteins enabled identification of 1,8-cineole-binding p450s. the p450 enzymes were cloned, heterologously expressed (n- ... | 2016 | 27590809 |
| efficient hydroxylation of 1,8-cineole with monoterpenoid-resistant recombinant pseudomonas putida gs1. | in this work, monoterpenoid hydroxylation with pseudomonas putida gs1 and kt2440 were investigated as host strains, and the cytochrome p450 monooxygenase cyp176a1 (p450cin) and its native redox partner cindoxin (cinc) from citrobacter braakii were introduced in p. putida to catalyze the stereoselective hydroxylation of 1,8-cineole to (1r)-6β-hydroxy-1,8-cineole. growth experiments in the presence of 1,8-cineole confirmed pseudomonads' superior resilience compared to e. coli. whole-cell p. putida ... | 2016 | 27263007 |
| pqsbc, a condensing enzyme in the biosynthesis of the pseudomonas aeruginosa quinolone signal: crystal structure, inhibition, and reaction mechanism. | pseudomonas aeruginosaproduces a number of alkylquinolone-type secondary metabolites best known for their antimicrobial effects and involvement in cell-cell communication. in the alkylquinolone biosynthetic pathway, the β-ketoacyl-(acyl carrier protein) synthase iii (fabh)-like enzyme pqsbc catalyzes the condensation of octanoyl-coenzyme a and 2-aminobenzoylacetate (2-aba) to form the signal molecule 2-heptyl-4(1h)-quinolone. pqsbc, a potential drug target, is unique for its heterodimeric arrang ... | 2016 | 26811339 |
| data on the standardization of a cyclohexanone-responsive expression system for gram-negative bacteria. | engineering of robust microbial cell factories requires the use of dedicated genetic tools somewhat different from those traditionally used for laboratory-adapted microorganisms. we have edited and formatted the chnr/p chnb regulatory node of acinetobacter johnsonii to ease the targeted engineering of ectopic gene expression in gram-negative bacteria. the proposed compositional standard was thoroughly verified with a monomeric and superfolder green fluorescent protein (msf•gfp) in escherichia co ... | 2016 | 26870759 |
| evaluation of the catalytic contribution from a positioned general base in ketosteroid isomerase. | proton transfer reactions are ubiquitous in enzymes and utilize active site residues as general acids and bases. crystal structures and site-directed mutagenesis are routinely used to identify these residues, but assessment of their catalytic contribution remains a major challenge. in principle, effective molarity measurements, in which exogenous acids/bases rescue the reaction in mutants lacking these residues, can estimate these catalytic contributions. however, these exogenous moieties can be ... | 2016 | 27410422 |
| structure of methionine γ-lyase from clostridium sporogenes. | methionine γ-lyase (mgl) is a pyridoxal 5'-phosphate-dependent enzyme that catalyzes the γ-elimination reaction of l-methionine. the enzyme is a promising target for therapeutic intervention in some anaerobic pathogens and has attracted interest as a potential cancer treatment. the crystal structure of mgl from clostridium sporogenes has been determined at 2.37 å resolution. the fold of the protein is similar to those of homologous enzymes from citrobacter freundii, entamoeba histolytica, pseudo ... | 2016 | 26750487 |
| mcpq is a specific citrate chemoreceptor that responds preferentially to citrate/metal ion complexes. | chemoreceptors are at the beginning of chemosensory pathways that mediate chemotaxis. pseudomonas putida kt2440 is predicted to have 27 chemoreceptors, most of which uncharacterized. we have previously identified mcps as chemoreceptor for krebs cycle intermediates. citrate is primarily present in the environment as metal complex, which, however, is not recognized by mcps. we show here that the mcps paralogue mcpq recognizes specifically citrate and citrate/metal(2+) complexes. the mcpq ligand bi ... | 2016 | 26463109 |
| direct detection of burkholderia cepacia in susceptible pharmaceutical products using semi-nested pcr. | burkholderia cepaciahas recently received a considerable attention as one of the major risks in susceptible pharmaceutical products. this microorganism can easily propagate and cause vast and severe contamination, especially to the water supplies for pharmaceutical companies. moreover, it proliferates within the products and can cause severe infections for humans. therefore, fast and sensitive detection of these bacteria is of a great demand. the present study introduces improved application of ... | 2016 | 26797972 |
| a loop-mediated isothermal amplification assay and sample preparation procedure for sensitive detection of xanthomonas fragariae in strawberry. | xanthomonas fragariae is a bacterium that causes angular leaf spot of strawberry. asymptomatic infection is common and contributes to the difficulties in disease management. the aim of this study was to develop a loop-mediated isothermal amplification (lamp) assay as an efficient method for detection of asymptomatic infections of x. fragariae. in addition, a new method of sample preparation was developed that allows sampling of a larger amount of plant tissue, hence increasing the detection rate ... | 2016 | 26766068 |
| activity enhancement based on the chemical equilibrium of multiple-subunit nitrile hydratase from bordetella petrii. | the maturation mechanism of nitrile hydratase (nhase) of pseudomonas putida nrrl-18668 was discovered and named as "self-subunit swapping." since the nhase of bordetella petrii dsm 12804 is similar to that of p. putida, the nhase maturation of b. petrii is proposed to be the same as that of p. putida. however, there is no further information on the application of nhase according to these findings. we successfully rapidly purified nhase and its activator through affinity his tag, and found that t ... | 2016 | 27075457 |
| bacterial flagellar motility on hydrated rough surfaces controlled by aqueous film thickness and connectedness. | recent studies have shown that rates of bacterial dispersion in soils are controlled by hydration conditions that define size and connectivity of the retained aqueous phase. despite the ecological implications of such constraints, microscale observations of this phenomenon remain scarce. here, we quantified aqueous film characteristics and bacterial flagellated motility in response to systematic variations in microhydrological conditions on porous ceramic surfaces that mimic unsaturated soils. w ... | 2016 | 26757676 |
| a hospital-wide outbreak of serratia marcescens, and ishikawa's "fishbone" analysis to support outbreak control. | a nosocomial outbreak of serratia marcescens in respiratory samples predominantly from patients in a surgical intensive care unit is reported. most of these patients were cardiac surgical patients. initially, a vigorous but inconclusive investigation was implemented on the basis of standardized (according the us centers for disease control and prevention) steps of outbreak investigation. then, a systemic quality management approach with "fishbone" analysis was added. as a consequence, plausible ... | 2016 | 26783861 |
| bromochloromethane, a methane analogue, affects the microbiota and metabolic profiles of the rat gastrointestinal tract. | bromochloromethane (bcm), an inhibitor of methanogenesis, has been used in animal production. however, little is known about its impact on the intestinal microbiota and metabolic patterns. the present study aimed to investigate the effect of bcm on the colonic bacterial community and metabolism by establishing a wistar rat model. twenty male wistar rats were randomly divided into two groups (control and treated with bcm) and raised for 6 weeks. bacterial fermentation products in the cecum were d ... | 2016 | 26567308 |
| diffusion of bacterial cells in porous media. | the chemotaxis signal transduction network regulates the biased random walk of many bacteria in favorable directions and away from harmful ones through modulating the frequency of directional reorientations. in mutants of diverse bacteria lacking the chemotaxis response, migration in classic motility agar, which constitutes a fluid-filled porous medium, is compromised; straight-swimming cells unable to tumble become trapped within the agar matrix. spontaneous mutations that restore spreading hav ... | 2016 | 26745427 |
| efficient and automated large-scale detection of structural relationships in proteins with a flexible aligner. | the total number of known three-dimensional protein structures is rapidly increasing. consequently, the need for fast structural search against complete databases without a significant loss of accuracy is increasingly demanding. recently, topsearch, an ultra-fast method for finding rigid structural relationships between a query structure and the complete protein data bank (pdb), at the multi-chain level, has been released. however, comparable accurate flexible structural aligners to perform effi ... | 2016 | 26732380 |
| a multi-substrate approach for functional metagenomics-based screening for (hemi)cellulases in two wheat straw-degrading microbial consortia unveils novel thermoalkaliphilic enzymes. | functional metagenomics is a promising strategy for the exploration of the biocatalytic potential of microbiomes in order to uncover novel enzymes for industrial processes (e.g. biorefining or bleaching pulp). most current methodologies used to screen for enzymes involved in plant biomass degradation are based on the use of single substrates. moreover, highly diverse environments are used as metagenomic sources. however, such methods suffer from low hit rates of positive clones and hence the dis ... | 2016 | 26822785 |
| threonine aldolases: perspectives in engineering and screening the enzymes with enhanced substrate and stereo specificities. | threonine aldolases have emerged as a powerful tool for asymmetric carbon-carbon bond formation. these enzymes catalyse the unnatural aldol condensation of different aldehydes and glycine to produce highly valuable β-hydroxy-α-amino acids with complete stereocontrol at the α-carbon and moderate specificity at the β-carbon. a range of microbial threonine aldolases has been recently recombinantly produced by several groups and their biochemical properties were characterized. numerous studies have ... | 2016 | 26810201 |
| substrate and cofactor range differences of two cysteine dioxygenases from ralstonia eutropha h16. | cysteine dioxygenases (cdos), which catalyze the sulfoxidation of cysteine to cysteine sulfinic acid (csa), have been extensively studied in eukaryotes because of their roles in several diseases. in contrast, only a few prokaryotic enzymes of this type have been investigated. in ralstonia eutropha h16, two cdo homologues (cdoa and cdob) have been identified previously. in vivo studies showed that escherichia coli cells expressing cdoa could convert 3-mercaptopropionate (3mp) to 3-sulfinopropiona ... | 2016 | 26590284 |
| isolation of polyvalent bacteriophages by sequential multiple-host approaches. | many studies on phage biology are based on isolation methods that may inadvertently select for narrow-host-range phages. consequently, broad-host-range phages, whose ecological significance is largely unexplored, are consistently overlooked. to enhance research on such polyvalent phages, we developed two sequential multihost isolation methods and tested both culture-dependent and culture-independent phage libraries for broad infectivity. lytic phages isolated from activated sludge were capable o ... | 2016 | 26590277 |
| bacteria induce pigment formation in the basidiomycete serpula lacrymans. | basidiomycete fungi are characterized ecologically for their vital functional role in ecosystem carbon recycling and chemically for their capacity to produce a diverse array of small molecules. chromophoric natural products derived from the quinone precursor atromentin, such as variegatic acid and involutin, have been shown to function in redox cycling. yet, in the context of an inter-kingdom natural system these pigments are still elusive. here, we co-cultured the model saprotrophic basidiomyce ... | 2016 | 27699944 |
| a preliminary study on the antibacterial mechanism of tegillarca granosa hemoglobin by derived peptides and peroxidase activity. | the blood clam, tegillarca granosa, is one of the few bivalve molluscs containing hemoglobin (hb). in the present study, we purified two types of t. granosa hemoglobin, tg-hbi and tg-hbii, using size exclusion chromatography and measured their antibacterial and peroxidase activities. we also tested antibacterial activities of peptides prepared by trypsin digestion of purified tg-hb and reversed-phase high-performance liquid chromatography purification. purified tg-hbi and tg-hbii showed antibact ... | 2016 | 26876330 |
| cd(ii) sorption on montmorillonite-humic acid-bacteria composites. | soil components (e.g., clays, bacteria and humic substances) are known to produce mineral-organic composites in natural systems. herein, batch sorption isotherms, isothermal titration calorimetry (itc), and cd k-edge exafs spectroscopy were applied to investigate the binding characteristics of cd on montmorillonite(mont)-humic acid(ha)-bacteria composites. additive sorption and non-additive cd(ii) sorption behaviour is observed for the binary mont-bacteria and ternary mont-ha-bacteria composite, ... | 2016 | 26792640 |
| silver nanoparticles formation by extracellular polymeric substances (eps) from electroactive bacteria. | microbial extracellular polymeric substances (eps) excreted from microorganisms were a complex natural biological polymer mixture of proteins and polysaccharides, which played an important roles in the transport of metals, such as ag(+). electroactive bacteria, is an important class of environmental microorganisms, which can use iron or manganese mineral as terminal electron acceptors to generate energy for biosynthesis and cell maintenance. in this work, the eps extracted of three electroactive ... | 2016 | 26797954 |
| mitochondrial carriers link the catabolism of hydroxyaromatic compounds to the central metabolism in candida parapsilosis. | the pathogenic yeast candida parapsilosis metabolizes hydroxyderivatives of benzene and benzoic acid to compounds channeled into central metabolism, including the mitochondrially localized tricarboxylic acid cycle, via the 3-oxoadipate and gentisate pathways. the orchestration of both catabolic pathways with mitochondrial metabolism as well as their evolutionary origin is not fully understood. our results show that the enzymes involved in these two pathways operate in the cytoplasm with the exce ... | 2016 | 27707801 |
| disruption of pseudomonas putida by high pressure homogenization: a comparison of the predictive capacity of three process models for the efficient release of arginine deiminase. | disruption of pseudomonas putida kt2440 by high-pressure homogenization in a french press is discussed for the release of arginine deiminase (adi). the enzyme release response of the disruption process was modelled for the experimental factors of biomass concentration in the broth being disrupted, the homogenization pressure and the number of passes of the cell slurry through the homogenizer. for the same data, the response surface method (rsm), the artificial neural network (ann) and the suppor ... | 2016 | 27699703 |
| plant drought tolerance enhancement by trehalose production of desiccation-tolerant microorganisms. | a collection of desiccation-tolerant xeroprotectant-producing microorganisms was screened for their ability to protect plants against drought, and their role as plant growth-promoting rhizobacteria was investigated in two different crops (tomato and pepper). the most commonly described biochemical mechanisms for plant protection against drought by microorganisms including the production of phytohormones, antioxidants and xeroprotectants were analyzed. in particular, the degree of plant protectio ... | 2016 | 27746776 |
| harnessing host-vector microbiome for sustainable plant disease management of phloem-limited bacteria. | plant health and productivity is strongly influenced by their intimate interaction with deleterious and beneficial organisms, including microbes, and insects. of the various plant diseases, insect-vectored diseases are of particular interest, including those caused by obligate parasites affecting plant phloem such as candidatus (ca.) phytoplasma species and several species of ca. liberibacter. recent studies on plant-microbe and plant-insect interactions of these pathogens have demonstrated that ... | 2016 | 27746788 |
| draft genome sequences of four hospital-associated pseudomonas putida isolates. | we present here the draft genome sequences of four pseudomonas putida isolates belonging to a single clone suspected for nosocomial transmission between patients and a bronchoscope in a tertiary hospital. the four genome sequences belong to a single lineage but contain differences in their mobile genetic elements. | 2016 | 27688339 |
| activation of the glutamic acid-dependent acid resistance system in escherichia coli bl21(de3) leads to increase of the fatty acid biotransformation activity. | the biosynthesis of carboxylic acids including fatty acids from biomass is central in envisaged biorefinery concepts. the productivities are often, however, low due to product toxicity that hamper whole-cell biocatalyst performance. here, we have investigated factors that influence the tolerance of escherichia coli to medium chain carboxylic acid (i.e., n-heptanoic acid)-induced stress. the metabolic and genomic responses of e. coli bl21(de3) and mg1655 grown in the presence of n-heptanoic acid ... | 2016 | 27681369 |
| structural and histone binding ability characterization of the arb2 domain of a histone deacetylase hda1 from saccharomyces cerevisiae. | hda1 is the catalytic core component of the h2b- and h3- specific histone deacetylase (hdac) complex from saccharomyces cerevisiae, which is involved in the epigenetic repression and plays a crucial role in transcriptional regulation and developmental events. though the n-terminal catalytic hdac domain of hda1 is well characterized, the function of the c-terminal arb2 domain remains unknown. in this study, we determine the crystal structure of the arb2 domain from s. cerevisiae hda1 at a resolut ... | 2016 | 27665728 |
| comprehensive genomic and phenotypic metal resistance profile of pseudomonas putida strain s13.1.2 isolated from a vineyard soil. | trace metals are required in many cellular processes in bacteria but also induce toxic effects to cells when present in excess. as such, various forms of adaptive responses towards extracellular trace metal ions are essential for the survival and fitness of bacteria in their environment. a soil pseudomonas putida, strain s13.1.2 has been isolated from french vineyard soil samples, and shown to confer resistance to copper ions. further investigation revealed a high capacity to tolerate elevated c ... | 2016 | 27730570 |
| molecular detection of acanthamoeba spp., naegleria fowleri and vermamoeba (hartmannella) vermiformis as vectors for legionella spp. in untreated and solar pasteurized harvested rainwater. | legionella spp. employ multiple strategies to adapt to stressful environments including the proliferation in protective biofilms and the ability to form associations with free-living amoeba (fla). the aim of the current study was to identify legionella spp., acanthamoeba spp., vermamoeba (hartmannella) vermiformis and naegleria fowleri that persist in a harvested rainwater and solar pasteurization treatment system. | 2016 | 27724947 |
| highly efficient and stable novel nanobiohybrid catalyst to avert 3,4-dihydroxybenzoic acid pollutant in water. | the present study reported for the first time covalent immobilization of protocatechuate 3,4-dioxygenase (3,4-pod) onto functionalized multi-walled carbon nanotubes (f-mwcnt) for degrading the toxic 3,4-dihydroxybenzoic acid (3,4-dhba) pollutant in water. the f-mwcnts had a maximum 3,4-pod loading of 1060 μg/mg. immobilized 3,4 pod had 44% of relative structural changes to its free configurations. nevertheless, >90% of relative activity and about 50% of catalytic efficiency were retained to the ... | 2016 | 27721429 |
| salicylic acid is involved in the basal resistance of tomato plants to citrus exocortis viroid and tomato spotted wilt virus. | tomato plants expressing the nahg transgene, which prevents accumulation of endogenous salicylic acid (sa), were used to study the importance of the sa signalling pathway in basal defence against citrus exocortis viroid (cevd) or tomato spotted wilt virus (tswv). the lack of sa accumulation in the cevd- or tswv-infected nahg tomato plants led to an early and dramatic disease phenotype, as compared to that observed in the corresponding parental money maker. addition of acibenzolar-s-methyl, a ben ... | 2016 | 27893781 |
| enhancing poly(3-hydroxyalkanoate) production in escherichia coli by the removal of the regulatory gene arca. | recombinant escherichia coli is a desirable platform for the production of many biological compounds including poly(3-hydroxyalkanoates), a class of naturally occurring biodegradable polyesters with promising biomedical and material applications. although the controlled production of desirable polymers is possible with the utilization of fatty acid feedstocks, a central challenge to this biosynthetic route is the improvement of the relatively low polymer yield, a necessary factor of decreasing t ... | 2016 | 27878786 |
| draft genome sequence of pseudomonas mosselii gil3, isolated from catfish and antagonistic against hypervirulent aeromonas hydrophila. | pseudomonas mosselii gil3 was isolated from a catfish that survived from lethal challenge with hypervirulent aeromonas hydrophila (vah). when assayed in vitro, the bacterium showed antagonism against vah. sequence analysis revealed that the genome of p. mosselii gil3 encodes numerous aromatic metabolism pathways and proteins for biosynthesis of antimicrobial compounds. | 2016 | 27856595 |
| two-step production of phenylpyruvic acid from l-phenylalanine by growing and resting cells of engineered escherichia coli: process optimization and kinetics modeling. | phenylpyruvic acid (ppa) is widely used in the pharmaceutical, food, and chemical industries. here, a two-step bioconversion process, involving growing and resting cells, was established to produce ppa from l-phenylalanine using the engineered escherichia coli constructed previously. first, the biotransformation conditions for growing cells were optimized (l-phenylalanine concentration 20.0 g·l-1, temperature 35°c) and a two-stage temperature control strategy (keep 20°c for 12 h and increase the ... | 2016 | 27851793 |
| flavin-dependent redox transfers by the two-component diketocamphane monooxygenases of camphor-grown pseudomonas putida ncimb 10007. | the progressive titres of key monooxygenases and their requisite native donors of reducing power were used to assess the relative contribution of various camphor plasmid (cam plasmid)- and chromosome-coded activities to biodegradation of (rac)-camphor at successive stages throughout growth of pseudomonas putida ncimb 10007 on the bicylic monoterpenoid. a number of different flavin reductases (frs) have the potential to supply reduced flavin mononucleotide to both 2,5- and 3,6-diketocamphane mono ... | 2016 | 27754389 |
| draft genome sequence of the marine rhodobacteraceae strain o3.65, cultivated from oil-polluted seawater of the deepwater horizon oil spill. | the marine alphaproteobacterium strain o3.65 was isolated from an enrichment culture of surface seawater contaminated with weathered oil (slicks) from the deepwater horizon (dwh) oil spill and belongs to the ubiquitous, diverse and ecological relevant roseobacter group within the rhodobacteraceae. here, we present a preliminary set of physiological features of strain o3.65 and a description and annotation of its draft genome sequence. based on our data we suggest potential ecological roles of th ... | 2016 | 27777651 |
| structure and catalytic mechanism of monodehydroascorbate reductase, mdhar, from oryza sativa l. japonica. | ascorbic acid (asa) maintains redox homeostasis by scavenging reactive oxygen species from prokaryotes to eukaryotes, especially plants. the enzyme monodehydroascorbate reductase (mdhar) regenerates asa by catalysing the reduction of monodehydroascorbate, using nadh or nadph as an electron donor. the detailed recycling mechanism of mdhar remains unclear due to lack of structural information. here, we present the crystal structures of mdhar in the presence of cofactors, nicotinamide adenine dinuc ... | 2016 | 27652777 |
| genome-based genetic tool development for bacillus methanolicus: theta- and rolling circle-replicating plasmids for inducible gene expression and application to methanol-based cadaverine production. | bacillus methanolicus is a thermophilic methylotroph able to overproduce amino acids from methanol, a substrate not used for human or animal nutrition. based on our previous rna-seq analysis a mannitol inducible promoter and a putative mannitol activator gene mtlr were identified. the mannitol inducible promoter was applied for controlled gene expression using fluorescent reporter proteins and a flow cytometry analysis, and improved by changing the -35 promoter region and by co-expression of the ... | 2016 | 27713731 |
| engineering of tm1459 from thermotoga maritima for increased oxidative alkene cleavage activity. | oxidative cleavage of alkenes is a widely employed process allowing oxyfunctionalization to corresponding carbonyl compounds. recently, a novel biocatalytic oxidative alkene cleavage activity on styrene derivatives was identified in tm1459 from thermotoga maritima. in this work we engineered the enzyme by site-saturation mutagenesis of active site amino acids to increase its activity and to broaden its substrate scope. a high-throughput assay for the detection of the ketone products was successf ... | 2016 | 27713741 |
| triclosan in water, implications for human and environmental health. | triclosan (tcs) is a broad spectrum antibacterial agent present as an active ingredient in some personal care products such as soaps, toothpastes and sterilizers. it is an endocrine disrupting compound and its increasing presence in water resources as well as in biosolid-amended soils used in farming, its potential for bioaccumulation in fatty tissues and toxicity in aquatic organisms are a cause for concern to human and environmental health. tcs has also been detected in blood, breast milk, uri ... | 2016 | 27722057 |
| biosynthesis and molecular actions of specialized 1,4-naphthoquinone natural products produced by horticultural plants. | the 1,4-naphthoquinones (1,4-nqs) are a diverse group of natural products found in every kingdom of life. plants, including many horticultural species, collectively synthesize hundreds of specialized 1,4-nqs with ecological roles in plant-plant (allelopathy), plant-insect and plant-microbe interactions. numerous horticultural plants producing 1,4-nqs have also served as sources of traditional medicines for hundreds of years. as a result, horticultural species have been at the forefront of many b ... | 2016 | 27688890 |
| physical forces shape group identity of swimming pseudomonas putida cells. | the often striking macroscopic patterns developed by motile bacterial populations on agar plates are a consequence of the environmental conditions where the cells grow and spread. parameters such as medium stiffness and nutrient concentration have been reported to alter cell swimming behavior, while mutual interactions among populations shape collective patterns. one commonly observed occurrence is the mutual inhibition of clonal bacteria when moving toward each other, which results in a distinc ... | 2016 | 27695443 |
| cloning, expression, and characterization of a peptidoglycan hydrolase from the burkholderia pseudomallei phage st79. | the lytic phage st79 of burkholderia pseudomallei can lyse a broad range of its host including antibiotic resistant isolates from within using a set of proteins, holin, lysb, lysc and endolysin, a peptidoglycan (pg) hydrolase enzyme. the phage st79 endolysin gene identified as peptidase m15a was cloned, expressed and purified to evaluate its potential to lyse pathogenic bacteria. the molecular size of the purified enzyme is approximately 18 kda and the in silico study cited here indicated the pr ... | 2016 | 27637947 |
| prevalence and antimicrobial resistance pattern of bacterial strains isolated from patients with urinary tract infection in messalata central hospital, libya. | to investigate the prevalence of urinary tract infection among patients at messalata central hospital, libya, to identify the causative bacteria, and to explore their resistance pattern to antimicrobials. | 2016 | 27569886 |
| application of cinder gel-beads/reeds combination strategy for bioremediation of pyrene- and indeno(1,2,3-cd)pyrene-contaminated estuarine wetlands. | pseudomonas putida pyr1 and acinetobacter baumannii inp1 isolated from liaohe estuarine wetlands were entrapped in cinder beads to make cinder gel-beads. they were combined with reeds for bioremediation of pyrene- and indeno(1,2,3-cd)pyrene-contaminated estuarine wetlands. the results showed that the removal percentages of pyrene and indeno(1,2,3-cd)pyrene (69.2 and 89.8 % respectively) in 40 days using cinder gel-beads/reeds were obviously higher than those using cinder gel-beads(52.6 and 70.0 ... | 2016 | 26897584 |
| clinical usefulness of arbekacin. | arbekacin is a broad-spectrum aminoglycoside used to treat methicillin-resistant staphylococcus aureus (mrsa). arbekacin has antibacterial activities against high-level gentamicin-resistant enterococci, multidrug-resistant pseudomonas aeruginosa, and acinetobacter baumannii et al. here, we reviewed in vitro data on arbekacin in staphylococci and gram-negative microorganisms. we also reviewed clinical studies for clinical efficacy and microbiologic efficacy data in patients with identified mrsa a ... | 2016 | 27104010 |
| integrated analysis of gene expression and metabolic fluxes in pha-producing pseudomonas putida grown on glycerol. | given its high surplus and low cost, glycerol has emerged as interesting carbon substrate for the synthesis of value-added chemicals. the soil bacterium pseudomonas putida kt2440 can use glycerol to synthesize medium-chain-length poly(3-hydroxyalkanoates) (mcl-pha), a class of biopolymers of industrial interest. here, glycerol metabolism in p. putida kt2440 was studied on the level of gene expression (transcriptome) and metabolic fluxes (fluxome), using precisely adjusted chemostat cultures, gro ... | 2016 | 27142075 |
| regio- and stereoselective aliphatic-aromatic cross-benzoin reaction: enzymatic divergent catalysis. | the catalytic asymmetric synthesis of chiral 2-hydroxy ketones by using different thiamine diphosphate dependent enzymes, namely benzaldehyde lyase from pseudomonas fluorescens (pfbal), a variant of benzoylformate decarboxylase from pseudomonas putida (ppbfd-l461a), branched-chain 2-keto acid decarboxylase from lactococcus lactis (llkdca) and a variant of pyruvate decarboxylase from acetobacter pasteurianus (appdc-e469g), was studied. starting with the same set of substrates, substituted benzald ... | 2016 | 27515897 |
| editorial: wired for life. | 2016 | 27242692 | |
| the preprotein translocase yidc controls respiratory metabolism in mycobacterium tuberculosis. | the yidc-oxa1-alb3 preprotein translocases play a vital role in membrane insertion of proteins in eukaryotes and bacteria. in a recent study we observed that rv3921c, which encodes putative yidc translocase in mycobacterium tuberculosis (mtb), is essential for in vitro growth of bacteria. however, the exact function of this particular protein remains to identify in mycobacterial pathogens. by performing a systematic study here we show that yidc of mtb is an envelope protein, which is required fo ... | 2016 | 27166092 |
| study on wastewater toxicity using toxtrak™ method. | toxtrak™ method is an analytical tool for the measurement of toxicity of drinking water, wastewater and natural water. it is based upon the estimation of the inhibitive effect on bacterial respiration processes. the main aim of this work was to test the applicability of toxtrak™ method in the assessment of wastewater toxicity in a full-scale wwtp in poland. in order to achieve it, the study was divided into two parts. first, the validation of toxtrak™ method was performed. second, wastewater tox ... | 2016 | 26832868 |
| rational design, synthesis, anti-hiv-1 rt and antimicrobial activity of novel 3-(6-methoxy-3,4-dihydroquinolin-1(2h)-yl)-1-(piperazin-1-yl)propan-1-one derivatives. | in the present study, fifteen novel 3-(6-methoxy-3,4-dihydroquinolin-1(2h)-yl)-1-(piperazin-1-yl)propan-1-one (6a-o) derivatives were designed as inhibitor of hiv-1 rt using ligand based drug design approach and in-silico evaluated for drug-likeness properties. designed compounds were synthesized, characterized and in-vitro evaluated for rt inhibitory activity against wild hiv-1 rt strain. among the tested compounds, four compounds (6a, 6b, 6j and 6o) exhibited significant inhibition of hiv-1 rt ... | 2016 | 27288643 |
| mechanism-specific and whole-organism ecotoxicity of mono-rhamnolipids. | biosurfactants like rhamnolipids are promising alternatives to chemical surfactants in a range of applications. a wider use requires an analysis of their environmental fate and their ecotoxicological potential. in the present study mono-rhamnolipids produced by a recombinant pseudomonas putida strain were analyzed using the green toxicology concept for acute and mechanism-specific toxicity in an ecotoxicological test battery. acute toxicity tests with the invertebrate daphnia magna and with zebr ... | 2016 | 26802344 |
| molecular and spectroscopic characterization of aspergillus flavipes and pseudomonas putida l-methionine γ-lyase in vitro. | pseudomonas putida l-methionine γ-lyase (ppmgl) has been recognized as an efficient anticancer agent, however, its antigenicity and stability remain as critical challenges for its clinical use. from our studies, aspergillus flavipes l-methionine γ-lyase (afmgl) displayed more affordable biochemical properties than ppmgl. thus, the objective of this work was to comparatively assess the functional properties of afmgl and ppmgl via stability of their internal aldimine linkage, tautomerism of pyrido ... | 2016 | 27796875 |
| psamm: a portable system for the analysis of metabolic models. | the genome-scale models of metabolic networks have been broadly applied in phenotype prediction, evolutionary reconstruction, community functional analysis, and metabolic engineering. despite the development of tools that support individual steps along the modeling procedure, it is still difficult to associate mathematical simulation results with the annotation and biological interpretation of metabolic models. in order to solve this problem, here we developed a portable system for the analysis ... | 2016 | 26828591 |
| rice-field drowning-associated pneumonia in which pseudomonas spp., aspergillus fumigatus, and cunninghamella sp. are isolated. | we herein report the case of an 84-year-old who developed pneumonia after drowning in a rice field. besides aspergillus fumigatus, many pathogens previously not reported in drowning-associated pneumonia (such as pseudomonas fluorescens, pseudomonas putida, nocardia niigatensis, and cunninghamella sp.) were isolated from his sputum. he received sulbactam/ampicillin, trimethoprim/sulfamethoxazole, voriconazole, levofloxacin and liposomal amphotericin b, but died due to respiratory failure. because ... | 2016 | 27041173 |
| characterization of a carbapenem-hydrolyzing enzyme, poxb, in pseudomonas aeruginosa pao1. | pseudomonas aeruginosa is an opportunistic pathogen often associated with severe and life-threatening infections that are highly impervious to treatment. this microbe readily exhibits intrinsic and acquired resistance to varied antimicrobial drugs. resistance to penicillin-like compounds is commonplace and provided by the chromosomal ampc β-lactamase. a second, chromosomally encoded β-lactamase, poxb, has previously been reported in p. aeruginosa. in the present work, the contribution of this cl ... | 2016 | 26621621 |
| preparation and biological activity of new collagen composites, part i: collagen/zinc titanate nanocomposites. | the aim of this investigation was to develop new antimicrobial collagen/zinc titanate (zntio3) biomaterials using a sol-gel cryogenic draying technology in keeping the native collagen activity. broad-spectrum antimicrobial activity was demonstrated against firmicutes (staphylococcus epidermidis, bacillus cereus, and candida lusitaniae) and gracilicutes (escherichia coli, salmonella enterica, and pseudomonas putida) microorganisms. the antimicrobial activity as well as the cytotoxicity were speci ... | 2016 | 27138724 |
| effect of fermented broth from lactic acid bacteria on pathogenic bacteria proliferation. | in this study, the effect that 5 fermented broths of lactic acid bacteria (lab) strains have on the viability or proliferation and adhesion of 7 potentially pathogenic microorganisms was tested. the fermented broth from lactococcus lactis c660 had a growth inhibitory effect on escherichia coli k92 that reached of 31%, 19% to pseudomonas fluorescens, and 76% to staphylococcus epidermidis. the growth of staph. epidermidis was negatively affected to 90% by lc. lactis 11454 broth, whereas the growth ... | 2016 | 26851857 |
| isolation and characterization of bacterial endophytes of curcuma longa l. | fourteen endophytic bacterial isolates were isolated from the rhizome of curcuma longa l. were characterized on the basis of morphology, biochemical characteristics and 16s rrna gene sequence analysis. the isolates were identified to six strains namely bacillus cereus (ecl1), bacillus thuringiensis (ecl2), bacillus sp. (ecl3), bacillus pumilis (ecl4), pseudomonas putida (ecl5), and clavibacter michiganensis (ecl6). all the strains produced iaa and solubilized phosphate and only two strains produ ... | 2016 | 28330130 |
| singly flagellated pseudomonas aeruginosa chemotaxes efficiently by unbiased motor regulation. | pseudomonas aeruginosais an opportunistic human pathogen that has long been known to chemotax. more recently, it has been established that chemotaxis is an important factor in the ability ofp. aeruginosato make biofilms. genes that allowp. aeruginosato chemotax are homologous with genes in the paradigmatic model organism for chemotaxis,escherichia coli however,p. aeruginosais singly flagellated ande. colihas multiple flagella. therefore, the regulation of counterclockwise/clockwise flagellar mot ... | 2016 | 27048795 |
| a 2-hydroxypyridine catabolism pathway in rhodococcus rhodochrous strain py11. | rhodococcus rhodochrous py11 (dsm 101666) is able to use 2-hydroxypyridine as a sole source of carbon and energy. by investigating a gene cluster (hpo) from this bacterium, we were able to reconstruct the catabolic pathway of 2-hydroxypyridine degradation. here, we report that in rhodococcus rhodochrous py11, the initial hydroxylation of 2-hydroxypyridine is catalyzed by a four-component dioxygenase (hpobcdf). a product of the dioxygenase reaction (3,6-dihydroxy-1,2,3,6-tetrahydropyridin-2-one) ... | 2016 | 26655765 |
| synthesis and evaluation of mannitol-based inhibitors for lipopolysaccharide biosynthesis. | antibiotic resistance is a serious threat against humankind and the need for new therapeutics is crucial. without working antibiotics, diseases that we thought were extinct will come back. in this paper two new mannitol bisphosphate analogs, 1,6-dideoxy-1,6-diphosphoramidate mannitol and 1,6-dideoxy-1,6-dimethansulfonamide mannitol, have been synthesized and evaluated as potential inhibitors of the enzyme gmhb in the biosynthesis of lipopolysaccharides. 1,6-dideoxy-1,6-diphosphoramidate mannitol ... | 2016 | 26981280 |
| novel rhizosphere soil alleles for the enzyme 1-aminocyclopropane-1-carboxylate deaminase queried for function with an in vivo competition assay. | metagenomes derived from environmental microbiota encode a vast diversity of protein homologs. how this diversity impacts protein function can be explored through selection assays aimed to optimize function. while artificially generated gene sequence pools are typically used in selection assays, their usage may be limited because of technical or ethical reasons. here, we investigate an alternative strategy, the use of soil microbial dna as a starting point. we demonstrate this approach by optimi ... | 2016 | 26637602 |
| [microbial composition of various surfaces of skin during develop- ment of atopic dermatitis based on data from maldi-tof mass-spectrometry identification method]. | study transformation of skin microflora during development of atopic dermatitis. | 2016 | 27228668 |
| mutation of the streptococcus gordonii thiol-disulfide oxidoreductase sdba leads to enhanced biofilm formation mediated by the ciarh two-component signaling system. | streptococcus gordonii is a commensal inhabitant of human oral biofilms. previously, we identified an enzyme called sdba that played an important role in biofilm formation by s. gordonii. sdba is thiol-disulfide oxidoreductase that catalyzes disulfide bonds in secreted proteins. surprisingly, inactivation of sdba results in enhanced biofilm formation. in this study we investigated the basis for biofilm formation by the δsdba mutant. the results revealed that biofilm formation was mediated by the ... | 2016 | 27846284 |
| adaptive evolution of phosphorus metabolism in prochlorococcus. | inorganic phosphorus is scarce in the eastern mediterranean sea, where the high-light-adapted ecotype hli of the marine picocyanobacterium prochlorococcus marinus thrives. physiological and regulatory control of phosphorus acquisition and partitioning has been observed in hli both in culture and in the field; however, the optimization of phosphorus metabolism and associated gains for its phosphorus-limited-growth (plg) phenotype have not been studied. here, we reconstructed a genome-scale metabo ... | 2016 | 27868089 |
| novel culturing techniques select for heterotrophs and hydrocarbon degraders in a subantarctic soil. | the soil substrate membrane system (ssms) is a novel micro-culturing technique targeted at terrestrial soil systems. we applied the ssms to pristine and diesel fuel spiked polar soils, along with traditional solid media culturing and culture independent 454 tag pyrosequencing to elucidate the effects of diesel fuel on the soil community. the ssms enriched for up to 76% of the total soil diversity within high diesel fuel concentration soils, in contrast to only 26% of the total diversity for the ... | 2016 | 27827405 |
| draft genome sequence of a multi-metal resistant bacterium pseudomonas putida ath-43 isolated from greenwich island, antarctica. | 2016 | 27877169 |