Publications

[marfan's syndrome complicated by fibrous-cavernous pulmonary tuberculosis and aspergillosis].		1982	6760187
saprophytic invasion of infarcted pulmonary tissue by aspergillus species.	saprophytic invasion of infarcted pulmonary tissue has received only passing reference in published reports. five patients with colonisation of pulmonary infarcts by aspergillus are described, with discussion of the clinical and histopathological features. the characteristic features were widespread invasion of dead tissue by fungal hyphae, in three cases with liquefaction resulting in the production of a cavitated lesion. there was no evidence that the vascular thrombosis and the consequent inf ...	1982	6760449
studies on the composition and antibacterial activity of uterine fluid from mares.	forty uterine fluid samples were obtained during oestrus and dioestrus of successive cycles from 4 mares classified as resistant to bacterial infection of the uterus. the flushings were assayed for peroxidase activity and ability to influence phagocytosis by polymorphonuclear neutrophils. uterine fluid volumes and protein concentrations and serum oestrogen values were similar during oestrus and dioestrus. serum progesterone concentrations were depressed during oestrus. equine neutrophils adheren ...	1982	6762436
[precipitins in sarcoidosis].		1982	6763093
[solid-phase radioimmunoassay for measurement of igg, iga and igm antibodies against aspergillus fumigatus].		1982	6763647
[comparative study of fungicidal activity in vitro of various quaternary ammonium compounds used in ophthalmology].	a study was conducted to evaluate the antifungal activity in vitro of 5 quaternary ammonium compounds. n cetylpyridinium was found to be particularly active against candida albicans and aspergillus fumigatus. the association of n cetylpyridinium and of neosynephrine, found in various eye drop compositions, resulted in potentation of the antifungal activity of this quaternary ammonium.	1982	6754796
[aspergilli and chronic lung diseases].	in this study, mycological examinations of morning sputum of patients with chronic bronchitis and bronchial asthma has been performed. specimens were also examined from patients with lobar pneumonia as a control. a fumigatus and a. niger has been isolated from patients chronic bronchitis in frequency of 4.5% and 0.8% respectively. these 2 fungi has also been isolated from patients with bronchial asthma in frequency of 24.5% (a. fumigatus) and 2.4% (a. niger). the frequency of isolation of a. fum ...	1982	6755197
[mycoses caused by fungal spores in indoor air].	fungal spores in indoor air may cause mycoses and allergies in man. the following different types of exposure are interesting from the epidemiological view-point: (a) the occupational exposure (work done with fungi-contaminated material), (b) the accidental exposure (formation and growing of fungi by constructive defects), and (c) the inconspicuous constant exposure (as part of lifestyle, e.g. by plants or small pets). it is stressed that the only way to characterize the indoor air quality with ...	1982	6764028
a comparison of surface and cytoplasmic antigens of aspergillus fumigatus in an enzyme-linked immunosorbent assay (elisa).		1982	6759936
antitumor effect of heat-killed aspergillus fumigatus mycelium in a mouse model.	a suspension of heat-killed aspergillus fumigatus mycelium inhibited the growth of a chemically-induced mouse bladder tumor (mbt). tumor growth was inhibited when the mycelium was injected into mice in a mixture with the tumor cells, when injected into growing tumors, and when introduced ip at the time tumor cells were injected into the hind leg muscle. in the concentrations that affected tumor growth no toxicity of the fungus preparation was observed. the fungal suspension was more effective ag ...	1982	6760960
isolation and ultrastructural examination of conidial wall components of coccidioides and aspergillus.	the role of the propagule in the initiation of fungal disease is not fully appreciated. knowledge of the nature of early interactions between infectious propagules and host tissue during onset of respiratory mycoses is particularly scanty. this stems partly from lack of sufficient data on the structure, chemical composition and immunological activity of the microbial surface. in this study, a technique is described for isolation and subsequent ultrastructural examination of the surface wall comp ...	1982	6764024
studies on aspergillus fumigatus from the human lungs.	on the basis of cultural, morphological and developmental studies of the two aspergillus isolates from the lungs of an aspergillosis patient, the taxonomic status of aspergillus phialiseptus is discussed. the elimination of the name a. phialiseptus is proposed as the isolates were found by the authors to belong to a. fumigatus.	1982	6750981
[antifungal agents in otorhinolaryngology].	numerous progresses are realized in the chemotherapy of mycoses, particularly in the field of deep mycoses due to 3 categories of systemic antifungal agents: polyenes (oral nystatin, oral and intravenous amphotericin b), 5-fluorocytosine (oral and intravenous) and imidazole derivatives (oral and intravenous miconazole, oral ketoconazole). the old drugs as nystatin and amphotericin b per os continue to have a remarkable effect in oro-pharyngeal candidosis, chiefly after sufficient local contact w ...	1982	7158918
hydrolysis of vegetable oils and triglycerides by thermotolerant and zoopathogenic species of aspergillus from nigerian palm produce.	the ability of aspergillus fumigatus fres. and aspergillus nidulans (eidam) wint obtained from nigerian palm produce to degrade vegetable oils and triglycerides and the production and activity of their extracellular lipases were studied. both species readily hydrolysed palm oil and palm kernel oil among others liberating free fatty acids in the process. good growth with mycelia production of both fungi were also recorded on the triglycerides used as sources of carbon at 37 degrees c with the bes ...	1982	7040975
isolation of 80s ribosomes from spores of aspergillus fumigatus and their antigenicity in rabbits.	ribosomes were obtained from spores of aspergillus fumigatus by mechanical disruption and differential centrifugation. the initial preparation (crude ribosomes) contained spore components which appeared to be broken fragments of plasmalemma with or without organelles. purified ribosomes free of membranous material were prepared by gel filtration chromatography on sepharose cl-4b. monomeric 80s ribosomes consisting of 40% protein and 60% rna, and morphologically characteristic of fungal ribosomes ...	1982	7040976
vaccination studies of aspergillosis in turkeys: subcutaneous inoculation with several vaccine preparations followed by aerosol challenge exposure.	five vaccines were prepared from an isolate of aspergillus fumigatus: a culture filtrate vaccine (i), a spore vaccine (ii), a mycelial vaccine (iii), and 2 germling vaccines (iv and v). the 2 germling vaccines were produced on different media. two experiments were conducted to test the vaccines for efficacy in groups of 21 turkey poults by giving each bird 2 subcutaneous inoculations of the respective vaccine at 1 and 2 weeks of age. the birds were challenge exposed to an aerosol of spores of a ...	1982	7041709
aspergillus fumigatus infection of the optic nerve with mycotic arteritis of cerebral vessels.	a 56-year-old caucasian male, with no obvious immune deficit or systemic disease, presented with unilateral loss of vision due to a. fumigatus infection involving the right optic nerve. there was no proptosis. despite fungal chemotherapy he developed central nervous system involvement with mycotic arteritis of the branches of the right middle cerebral artery, temporal lobe infarction and rupture of a mycotic aneurysm of the right internal carotid artery, and died. review of the literature concer ...	1982	7042514
[features of aspergillosis in ent. histopathology of fungal infections of the paranasal sinuses caused by aspergillus fumigatus (author's transl)].	fungal diseases of nasal and paranasal sinuses caused by aspergillus fumigatus are not rare. second to diagnosis in defined culture medium only, histological diagnosis can easily be achieved in haematoxilin-eosin-, and pas-stained samples of fungal masses in the sinuses. specific histological features of aspergillus fumigatus are described, especially the onion-skin like structure of the fungus balls, and their development discussed. out of seventy cases only one showed invasion of the mucosa of ...	1982	7042658
the antifungal activity of carrier peptides, l-arginyl-x-l-phenylalanine, containing amino acid antagonists or atypical non-biogenic d-amino acids in the central position.	eleven analogues of l-arginyl-d-allo-threonyl-l-phenylalanine, a naturally occurring peptide with antifungal activity, were synthesized. two tripeptides of the form l-arginyl-x-l-phenylalanine (x = p-f-dl-phenylalanine or m-f-dl-tyrosine) inhibited aspergillus fumigatus and aspergillus flavus. in comparison with the free antagonists, the tripeptide-bound antagonists were more active against candida-albicans-isolates which means that the amino acid sequence may serve as carrier function: it enhan ...	1982	7124161
[a case of aspergillotic meningoencephalitis associated with trigeminal neuralgia].	a case of aspergillotic meningoencephalitis associated with trigeminal neuralgia was reported. the patient, a 41-year-old female, was admitted to our hospital on nov. 20, 1977 with the chief complaint of right trigeminal neuralgia. on admission right facial paresthesia and right abducens palsy were found. the skull x-ray and tomogram showed enlargement of the right superior orbital fissure. ct scan revealed an irregular high density around the right superior orbital fissure. the examination of s ...	1982	7133307
amino acid sequence homologies in alfa-sarcin, restrictocin and mitogillin.		1982	7150290
functional studies on mouse macrophages activated by phospholipids from listeria monocytogenes and aspergillus fumigatus.	the administration of phospholipids from listeria monocytogenes and aspergillus fumigatus to mice resulted in the development of macrophages with increased functional activity. it has been found that stimulated macrophages have increased phagocytic activity, enhanced chemotactic reactivity and augmented adherence to plastic surface. the activity of fcig macrophages and complement receptors as well as the rate of the recovery of the fcig receptors after phagocytosis were significantly higher in a ...	1983	6422902
determination of antibodies to partially purified aspergillus antigens by an enzyme-linked immunosorbent assay.	a total of 75 sera from 17 patients with pulmonary aspergillosis and 58 controls were investigated by elisa for igg antibodies against different antigen fractions of aspergillus fumigatus. somatic antigen obtained by homogenization of young hyphi was fractionated on a sephadex g-200 column, and 11 antigen fractions (i-xi) were prepared. antibodies to fractions i-xi were detected in sera from patients with aspergillosis, whereas antibodies in sera from control patients and healthy blood donors we ...	1983	6337106
development of fluorescent-antibody reagents for demonstration of pseudallescheria boydii in tissues.	we prepared fluorescent-antibody reagents to detect and identify pseudallescheria boydii in tissue. antisera to broken mycelium and condidia (particulate antigens) and to culture filtrates (soluble antigens) of p. boydii were produced in rabbits. antisera and globulin fractions of the antisera were labeled with fluorescein isothiocyanate and evaluated for their ability to stain p. boydii in tissues. the conjugates were first tested with cultures of 25 p. boydii isolates and 26 heterologous fungi ...	1983	6195179
rapid hematogenous dissemination of aspergillus fumigatus and a. flavus spores in turkey poults following aerosol exposure.	cultures of blood samples taken from 3-week-old turkey poults exposed to aerosolized spores of either aspergillus fumigatus or a. flavus for 15 min yielded organisms. aspergillus fumigatus was also isolated from brain and liver tissue samples taken immediately after exposure. exfoliated cells from the lungs of 10 poults exposed to aerosolized spores of a. fumigatus were allowed to attach to glass slides in tissue culture. several types of the fixed and stained cells had attached or ingested spor ...	1983	6418131
total serum ige levels in venezuelan schoolchildren.	seventy-eight disease-free children were evaluated by prist for total serum ige in order to establish the normal range for this immunoglobulin and assess its utility, in tropical climates, such as venezuela, as a diagnostic tool for clinical allergy. seventy-eight normals were selected from a group of 1053 children, aged 7-12 years from fourteen schools in caracas. exclusion from the normal pool was based on nationality, and on historical, clinical, and/or laboratory evidence of atopic and/or in ...	1983	6640887
[cerebral aspergillosis: presentation of a case].		1983	6391387
aspergillosis of the cerebral ventricles in a heroin abuser. case report and review of the literature.	aspergillosis of the cerebral ventricles developed in a 36-year-old heroin abuser who died during the sixth week of illness. the diagnosis was not made during life. a review of our case and of four previous cases from the literature indicates that aspergillosis should be considered in parenteral drug abusers with the syndrome of chronic meningitis or ventriculitis. the diagnosis is difficult to establish, but serologic tests may be helpful. this form of cns aspergillosis can be cured with antifu ...	1983	6401419
computer-assisted rapid enzyme-linked immunosorbent assay (elisa) in the serological diagnosis of aspergillosis.	a computer-assisted method for the positive/negative discrimination of elisa data is described. this method was applied to a rapid elisa procedure for igg class antibodies to aspergillus fumigatus in which the performance time of the test was reduced to 1 h. the method gives results which compare well with those by agar gel double diffusion (agdd). the computer-assisted reading, calculation and tabulation of elisa results from one microtitre plate is performed in less than 2 min and permits anal ...	1983	6402546
persistent dyspnea in a man with cystic fibrosis.		1983	6404806
efficacy of enzyme-linked immunosorbent assay in serodiagnosis of aspergillosis.	sera from 43 cases of aspergillosis, 73 cases of other bronchopulmonary diseases, and 50 healthy persons were examined for the presence of antibodies to aspergillus fumigatus. enzyme-linked immunosorbent assay proved to be more efficacious than the immunodiffusion test and counterimmunoelectrophoresis in the cases of allergic bronchopulmonary and invasive aspergillosis.	1983	6406541
exoantigen tests for the immunoidentification of fungal cultures.	exoantigen tests for the immunoidentification of fungal pathogens are playing a new and significant role in the diagnostic laboratory. properly performed and controlled exoantigen tests lead to rapid, accurate identification of cultures of many fungal pathogens. the tests are particularly valuable in identifying dimorphic pathogens that are difficult to convert or with atypical cultures. we review the value of exoantigen tests for identifying mycelial form fungi: aspergillus spp. blastomyces der ...	1983	6410243
"haysickness" in icelandic horses: precipitin tests and other studies.	blood samples were taken from 18 healthy horses (group a), 15 horses clinically diagnosed to have "haysickness" ("farmer's lung") (group b), 10 closely related horses (group c) and 14 inbred horses (group d). precipitins in sera were measured by double gel diffusion test against micropolyspora faeni, thermoactinomyces vulgaris, aspergillus fumigatus, alternaria, penicillium and rhizopus species. in group a, all the horses were precipitin negative except one with a faint reaction to rhizopus spec ...	1983	6411460
comparison of antibody measurements against aspergillus fumigatus by means of double-diffusion and enzyme-linked immunosorbent assay (elisa).	igg antibody titers against aspergillus fumigatus from different sera were measured by means of a standardized elisa and compared with precipitates measured by double diffusion (d.d.). there was a significant correlation between the number of precipitates and the elisa igg titers in the 758 sera examined. however, in several individual sera within this group, large deviations between these two immunologic parameters were found. further analysis indicated that elisa detects antibodies against non ...	1983	6411795
[aspergillus infection of severe burns].	aspergillus, which has a saprophytic life, is an opportunistic fungus and manifests his pathogenicity when a weakened state of the host facilitates its development. the conditions are particularly favorable in severely burned patients like the 54-year-old woman presented here. the criteria of pathogenicity of this opportunistic fungus, the incidence of epidemiology and prognosis of this type of infection and the clinical signs that allow an early diagnosis are discussed here.	1983	6414854
[aspergillus mycosis of the paranasal sinuses. roentgen diagnosis, clinical course and histopathology].		1983	6415447
aspergillus endophthalmitis in an intravenous drug user.	a 27-year-old male drug user administered methamphetamine hydrochloride solution intravenously (iv) from a storage vial. within two weeks he was admitted with a fungal endophthalmitis of the right eye. a diagnostic and therapeutic vitrectomy was performed. aspergillus fumigatus was cultured from the drug storage vial and the vitreous biopsy specimen. the endophthalmitis was not controlled by iv and intravitreal amphotericin b. pathological examination revealed fungal abscesses in the vitreous an ...	1983	6418052
superinfections in herpes simplex keratitis.	we reviewed 15 cases of culture-proven corneal superinfections in 15 patients (eight men and seven women ranging in age from 41 to 86 years) with recurrent herpes simplex keratitis. the factors that appeared to increase the risk of superinfection were the presence of an epithelial defect (found in all 15 cases), a history of recurrent herpetic keratouveitis (found in ten cases), and the use of topical corticosteroids (found in 13 cases). eight of the 15 patients were taking antibiotics at the ti ...	1983	6614114
the diagnostic approach to immediate hypersensitivity in patients with allergic rhinitis; a comparison of nasal challenges and serum rast.	nasal challenges performed in 182 patients with allergic rhinitis revealed 273 positive and 125 negative responses to house dust, grass pollen, cat and dog danders and aspergillus fumigatus, which all correlated with disease history and intracutaneous tests. in these randomly selected patients the specific ige antibodies in the serum to the same allergens as those used for nasal challenges were determined quantitatively by serum rast. additionally, blood and nasal eosinophilia were recorded duri ...	1983	6614602
minimal inhibitory concentrations of lucknomycin, a new polyenic derivative, for candida and aspergillus spp.	the minimal inhibitory concentrations (mics) of lucknomycin, a new polyenic derivative, were determined for 101 clinical isolates of candida, 38 clinical or environmental strains of aspergillus fumigatus, and 30 isolates of a. niger. the most susceptible species were candida albicans and candida tropicalis (mean mic, 0.4 micrograms/ml). aspergillus spp. were less susceptible, with mean mics of 0.60 micrograms/ml for aspergillus niger and 9.2 micrograms/ml for aspergillus fumigatus.	1983	6625552
killing of aspergillus spores depends on the anatomical source of the macrophage.	to resolve the controversy over the capacity of macrophages to kill or inhibit germination of aspergillus spores, we compared this function in peritoneal and alveolar macrophages. alveolar macrophages from rabbits killed 82 to 90% and completely digested 72 to 82% of spores of aspergillus fumigatus in 30 h. in contrast, peritoneal macrophages could not even inhibit the germination of ingested spores; more than 85% transformed into mycelia within 24 h. killing by alveolar macrophages was delayed ...	1983	6642661
pulmonary fibrosis, with sarcoid granulomas and angiitis, associated with handling of mouldy lichen.	we report a case of extrinsic allergic bronchiolo-alveolitis associated with handling of mouldy lichen. the patient had periodic dyspnoea, cough and mucoid sputum, and a micronodular infiltration of both lungs with slight restrictive ventilatory insufficiency. an open lung biopsy showed interstitial inflammation and fibrosis with sarcoid granulomas and angiitis. the symptoms disappeared during long term corticosteroid therapy, but reappeared every year when the patient participated in the handli ...	1983	6653692
intrinsic polymorphonuclear chemotactic defect in a boy with chronic granulomatous disease.	a six year old boy is described who suffured from recurrent and protracted infections of multiple organs by various catalase positive bacteria. a severe episode of osteomyelitis involving several bones was caused by aspergillus fumigatus. studies of his pmns revealed impaired metabolic as well as microbicidal functions characteristic of cgd. chemiluminescence in response to both opsonized zymosan and sodium fluoride was markedly depressed, while control pmns showed significant responses. control ...	1983	6670661
invasive aspergillosis in renal transplant recipients: correlation with corticosteroid therapy.	during a 31-month period in 1979-1981, nine patients at a renal transplant center in tennessee developed invasive infections with aspergillus species. despite an extensive search, no common environmental source of contamination was found. a matched case-control study of host risk factors showed that leukopenia, prior administration of antibiotics, and treatment with azathioprine and antilymphocyte serum were not significantly related to the development of aspergillosis. in contrast, the administ ...	1983	6350492
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 38-1983. empyema 40 years after a thoracoplasty.		1983	6350875
[pulmonary aspergillosis with abscess formation].		1983	6351394
keratomycosis in a buffalo calf (bubalus bubalis) caused by aspergillus fumigatus.		1983	6351411
mycotic invasion of the mare's uterus.	uterine infections in three mares following repeated uterine manipulations and antibiotic therapy were caused by aspergillus fumigatus in two cases and candida albicans in the third. two of the infections were noticed during dioestrus and one at oestrus and one mare produced a purulent vulval discharge. large numbers of neutrophils, increased protein concentrations and the presence of fungal hyphae in flushings from the uterine lumen were indicative of active infection. the mycotic infections ca ...	1983	6351412
circulating immune complexes and rheumatoid factors in patients with farmer's lung.	circulating igg immune complexes (cics), igm rheumatoid factors (rfs), complement level (c3 and c4), and igg antibodies to aspergillus fumigatus, aspergillus umbrosus, thermoactinomyces vulgaris, and micropolyspora faeni were analysed in the sera of 14 patients with farmer's lung (fl), 10 in the acute and four in the subacute phase of the disease. ten spouses of fl patients served as exposed healthy controls. c3 and c4 were measured fluoronephelometrically. c3 levels were above the normal range ...	1983	6351661
purification of the major endoglucanase from aspergillus fumigatus fresenius.	aspergillus fumigatus (fresenius), imi 246651, a.t.c.c. 46324, produces two beta-glucosidase enzymes, cotton-solubilizing activity, xylanase and endoglucanase enzymes which can be separated by gel-filtration chromatography. the major endoglucanase does not bind to concanavalin a-sepharose and does not stain with periodic acid/schiff reagent. it is homogeneous on polyacrylamide isoelectric focusing (pi = 7.1) and has a mol.wt. of 12500 by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis ...	1983	6351841
pathogenicity of aspergillus fumigatus in chicks, guinea fowl, rabbits and mice.		1983	6353224
role of endotoxin of aspergillus fumigatus in its pathogenicity.		1983	6353225
intracerebral aspergillus abscess: case report.	intracranial aspergillosis is rare and often fatal. the authors report the successful treatment of a right temporal intracerebral aspergillus abscess.	1983	6353266
orbital aspergillosis.	we treated a 40-year-old male who had orbital aspergillosis and presumed early intracranial extension with limited orbital exenteration and adjunctive amphotericin b. forty-two months later the patient is alive without recurrence. our patient illustrates the typical presentation of orbital aspergillosis with severe periorbital pain and proptosis; however, he did not have the usual concomitant sinusitis. aggressive surgical and antifungal therapy in this case was successful in preventing further ...	1983	6343937
[photocoagulation of mycotic fundus changes in rabbits].		1983	6361363
localized invasive pulmonary aspergillosis treated by surgical excision.	invasive aspergillosis developed in a patient without preexisting lung disease or immunosuppression, after she had received only a one-month course of corticosteroid therapy. surgical excision, without antifungal therapy, resulted in eradication of the infection.	1983	6353603
recovery of aspergillus fumigatus aerospora from municipal sewage sludge composting operations in the state of maine.	an investigation was conducted to determine the effect of composting activity on ambient and transient viable aspergillus fumigatus aerospora at three composting operations (westbrook, bangor, old town) in the state of maine. meteorological conditions and seasonality were also considered. the purpose of the study was to determine whether composting operations caused increased public health risks due to the generation and dispersal of elevated a. fumigatus aerospora concentrations. ambient aerosp ...	1983	6355853
[pleural aspergillosis].		1983	6356126
comparison of partially purified mycelial and culture filtrate antigens of aspergillus fumigatus by enzyme-linked immunosorbent assay.	an aqueous extract of aspergillus fumigatus mycelium was fractionated by precipitation with ammonium sulphate to give protein-enriched and carbohydrate-enriched preparations. the antigenic activity of the fractions so obtained was assessed in an enzyme-linked immunosorbent assay using pooled positive and negative reference sera. the best discrimination between such sera was obtained when using either the fraction insoluble in 50--75% ammonium sulphate or the material soluble in a saturate soluti ...	1983	6356408
serologic testing of wild roe deer (capreolus capreolus l.) from the trois fontaines forest region of eastern france.		1983	6358537
studies on experimental aspergillosis in immunodeficient mice.	mice with either chemically suppressed immune response or with specifically induced t cell or b cell immunodeficiency were challenged with aspergillus fumigatus spores. chemical immunosuppressants used were cortisone, cyclophosphamide or silica. t cell deficiency was produced by thymectomy and als administration, whereas, b cell deficiency was produced by administration of anti-mouse igm antibodies in newborn mice. the susceptibility of such animals to a. fumigatus challenge was monitored by mor ...	1983	6326425
antigens of aspergillus fumigatus. ii. electrophoretic and clinical studies.	cell sap (cs) and culture filtrate (cf) preparations of aspergillus fumigatus strains ag-507, ag-515, and ag-534 were analysed by two dimensional electrophoresis (2-de; i.e., first dimension isoelectric focusing, second dimension sodium dodecyl sulphate gradient pore gel), which enabled detection of strain- and species-specific components. in cs preparations it was shown that cs2, a fraction isolated from strain ag-507 by gel filtration, consists of the major protein components in the cs of the ...	1983	6347877
[study of fungal flora of the skin of patients with psoriasis].		1983	6361912
influence of econazole and 5-fluorocytosine on the ultrastructure of aspergillus fumigatus and the cytochemical localization of calcium ions as measured by laser microprobe mass analysis.	the ultrastructural changes and the localization of ca++ ions have been investigated in aspergillus fumigatus after exposure in vitro to econazole (50 micrograms ml-1) and to 5-fluorocytosine (100 micrograms ml-1) for 24 h. the changes obtained with econazole concerned the cell periphery, necrotization of mitochondria and hyperactivity of the central vacuole. after cytochemical ca++ localization a marked increase in precipitate was observed on mitochondria, vacuoles and collapsed membranes, comp ...	1983	6362038
[pilot study on the incidence of aspergillus fumigatus in in mucoviscidosis patients].		1983	6359121
antigen detection in the diagnosis of invasive aspergillosis. utility in controlled, blinded trials.	two blinded, controlled trials were done to evaluate the usefulness of fungal antigen detection for the diagnosis of invasive aspergillosis. detection of aspergillus fumigatus carbohydrate by radioimmunoassay was compared with antibody detection by an enzyme-linked immunosorbent assay and with diagnostic microbiologic and histopathologic procedures. in the first trial, antigenemia was detected in 4 of 6 leukemic patients with invasive pulmonary aspergillosis, but not in 8 acute leukemic controls ...	1983	6359996
[detection of anti-aspergillus igg type antibody in the sera by solid phase immunoradiometric assay--comparison with gel precipitation test in cases of pulmonary aspergilloma].		1983	6363780
mycotic abortions in equines.		1983	6363920
aspergillus terreus endocarditis--a case report.		1983	6368727
immediate-type reactions in patients with allergic bronchopulmonary aspergillosis.	allergic features of 38 patients with allergic bronchopulmonary aspergillosis (abpa) were reviewed. these features included skin reactivity to other inhalant antigens and to molds other than aspergillus fumigatus (af) plus clinical manifestations of rhinitis, conjunctivitis, asthma, eczema, urticaria, anaphylaxis, food allergy, and drug allergy. abpa patients have a high degree of allergic reactivity in all these clinical features, in particular, clearly documented food allergy. these findings d ...	1983	6853923
a radioimmunoassay index for allergic bronchopulmonary aspergillosis.	differentiating patients with allergic bronchopulmonary aspergillosis from those with asthma who have immediate cutaneous reactivity to aspergillus fumigatus may be difficult. both groups have ige and igg antibodies against aspergillus antigen. we describe methods for establishing ige and igg antibody indices using sera from asthmatic patients as a control serum pool. the ige and igg antibody indices are usually both positive and at least one index was positive in all patients during the acute s ...	1983	6859719
antibodies against aspergillus fumigatus in bronchial secretions from patients without aspergillosis.	antibodies to aspergillus fumigatus were investigated by indirect immunofluorescence (iif) and enzyme-linked immunosorbent assay (elisa) in bronchial secretions (bs) and sera from 40 patients with neoplasia, lung infiltrates of non-fungal aetiology or chronic bronchitis. by iif, igg aspergillus antibodies were found in 23 samples of bs (58%) (titre range 10-40) and in all 40 sera (titre range 10-1280). iga aspergillus antibodies were detected in 33 samples of bs (83%) (titre range 10-160) and in ...	1983	6880743
marijuana smoking and fungal sensitization.	the possible role of marijuana (mj) in inducing sensitization to aspergillus organisms was studied in 28 mj smokers by evaluating their clinical status and immune responses to microorganisms isolated from mj. the spectrum of illnesses included one patient with systemic aspergillosis and seven patients with a history of bronchospasm after the smoking of mj. twenty-one smokers were asymptomatic. fungi were identified in 13 of 14 mj samples and included aspergillus fumigatus, a. flavus, a. niger, m ...	1983	6833678
serum ige and ige antibody levels in patients with bronchial asthma, atopic dermatitis, eosinophilic granulomas of the soft tissue (kimura's disease) and other diseases.	hyper-ige immunoglobulinemia was observed in bronchial asthma, atopic dermatitis, eosinophilic granuloma of the soft tissue (kimura's disease), disseminated visceral eosinophilic granulomas (zuelzer-apt syndrome) and disseminated eosinophilic collagen disease, ige antibodies to environmental allergens (dermatophagoides farinae mites, aspergillus fumigatus, mountain cedar pollen, and candida albicans) were found in bronchial asthma, atopic dermatitis and combinations of the two, but were not sign ...	1983	6600385
allergic bronchopulmonary aspergillosis: reactivity of ige and igg antibodies with antigenic components of aspergillus fumigatus (ige/igg antigen complexes).	sera of patients with abpa were tested by xrie tests incorporating their own serum (self-xrie) to detect the presence of igg/ige antigen complexes to a "reference" aspergillus fumigatus preparation. of the 32 sera studied, 29 (90%) had visible precipitin (igg) peaks, and 27 of these 29 as well as the three apparently precipitin-negative sera, i.e., 30 (94%), showed binding of specific ige by autoradiography. the two precipitin-positive sera that did not show ige binding were also skin test negat ...	1983	6605984
airborne bacteria, endotoxin and fungi in dust in poultry and swine confinement buildings.	airborne dust in swine and poultry confinements was analyzed to determine concentrations of total and gram-negative bacteria, total fungi, aspergillus fumigatus and endotoxin. airborne concentrations of total and gram-negative bacteria in swine and confinement units have been found to be as high as, or higher, than those found in other environments, such as wastewater treatment plants and cotton card rooms, where microbiologically contaminated organic dusts were present. airborne endotoxin conce ...	1983	6613856
mechanisms of destruction of aspergillus fumigatus hyphae mediated by human monocytes.	normal monocytes attached to and destroyed aspergillus hyphae by morphologic and metabolic criteria. inhibition by anaerobiosis, azide, cyanide, halide-free conditions, catalase, histidine, and tryptophan suggested mediation of hyphal damage primarily through the myeloperoxidase system. however, myeloperoxidase-independent oxidative or nonoxidative mechanisms appeared active in hyphal damage by monocytes from patients with myeloperoxidase deficiency or chronic granulomatous disease (cgd). moreov ...	1983	6300255
treatment of experimental murine aspergillosis with bay n7133.	bay n7133, a recently developed imidazole antifungal drug, was evaluated in experimental murine aspergillosis. balb/c mice were challenged with conidia of aspergillus fumigatus by either the intravenous or the intranasal route. one day after challenge, oral treatment with bay n7133, ketoconazole, or a placebo control was begun bay n7133 was well absorbed after oral administration and penetrated all tissues evaluated. there was some evidence for accelerated inactivation of the drug after prolonge ...	1983	6313822
antigens/allergens of aspergillus fumigatus. identification of antigenic components reacting with both igg and ige antibodies of patients with allergic bronchopulmonary aspergillosis.	evidence that both ige and igg antibodies present in the sera of allergic bronchopulmonary aspergillosis patients (abpa) combine with several of the antigenic components of aspergillus fumigatus has been obtained using a self-crossed radioimmunoelectrophoresis test. in this test, patient's serum was used to develop its own crossed immunoprecipitation pattern to a reference antigen before proceeding to autoradiography with 125i-anti-human ige in the usual way. comparison of autoradiograph with im ...	1983	6192954
hyperreactivity of mediator-releasing cells from patients with allergic bronchopulmonary aspergillosis as evidenced by basophil histamine release.	histamine release was performed on 28 patients with allergic bronchopulmonary aspergillosis (abpa) in various stages and on 14 patients with mold-sensitive asthma, by using aspergillus antigens, other mold antigens, and anti-ige. total serum ige, ige and igg antibodies to aspergillus fumigatus (af), and end point cutaneous titration for af were determined for each patient. there was greater histamine release to aspergillus mix (p = 0.0000) and anti-ige (p = 0.047) in patients with abpa than in p ...	1983	6194196
effects of interferon-alpha on cytomegalovirus reactivation syndromes in renal-transplant recipients.	we have previously demonstrated that six weeks of prophylaxis with interferon-alpha delays cytomegalovirus excretion and decreases viremia in recipients of kidney transplants. in a double-blind trial to evaluate the effects of a longer course of prophylaxis, we gave either 3 x 10(6) units of interferon or placebo intramuscularly to 42 patients before transplant surgery was performed. after surgery, doses were given three times a week for six weeks and then twice a week for eight weeks (total of ...	1983	6304513
[disseminated aspergillosis in chronic respiratory insufficiency. diagnostic and therapeutic aspects].		1983	6226984
immune complex crescentic glomerulonephritis associated with pulmonary aspergillosis.	we describe a case of immune complex crescentic glomerulonephritis associated with invasive pulmonary aspergillus fumigatus infection. immunofluorescent and immunoperoxidase investigations showed the presence of aspergillus fumigatus antigen in the glomerular immune complexes.	1983	6229467
use of aspergillus fumigatus mycelial antigens in enzyme-linked immunosorbent assay and counter-immunoelectrophoresis.	antigens obtained from the ruptured mycelium of aspergillus fumigatus were separated on the basis of their attachment or non-attachment to concanavalin a-linked sepharose gel. they were analysed by double-diffusion and two-dimensional immunoelectrophoresis with rabbit antiserum and sera from patients with aspergillus-related disease. their sensitivity was assessed in an enzyme-linked immunosorbent assay (elisa) for immunoglobulin g. sera which gave very low or high extinction readings on elisa g ...	1983	6337264
invasive pulmonary aspergillosis associated with aspergilloma in sarcoidosis.	we have described two patients with corticosteroid-treated sarcoidosis and with autopsy-proven aspergilloma and diffuse invasive pulmonary aspergillosis. in one, growth of the fungus within a cyst was probably followed by progression to diffuse pulmonary infection. in the second, lung necrosis from invasive infection may have led to the formation of the mycetoma found at autopsy.	1983	6337409
[the litter - aspergillus - mastitis].	a number of cases of bovine mastitis caused by aspergillus fumigatus are reported. the animals showed considerably swollen hard quarters associated with the production of large quantities of watery secretion and symptoms of systemic disease. both intramammary and systemic treatment with several antibacterial agents failed to produce any improvement, nor did an antimycotic administered by intramammary route result in improvement. severe mastitis marked by mycotic lesions was observed on slaughter ...	1983	6338618
immunological significance of aspergillus fumigatus in cane-sugar mills.	an aeromycological study has revealed the preponderance of aspergillus fumigatus at the bagasse-containing sites in sugar-cane mills located in the northwestern part of the punjab state. the fungus was frequently isolated from the sputum cultures of the mill workers and a significant number of the workers were positive reactors when challenged intracutaneously with aspergillin. immediate type i and late arthus type skin hypersensitivity was observed in 10.6% and 4.4%, respectively, of the 548 wo ...	1983	6338838
aspergillus fumigatus endocarditis on a normal heart valve.	we have described an elderly man with no known underlying predisposing systemic or valvular disease who had mitral valve infection and endophthalmitis due to aspergillus fumigatus. two-dimensional echocardiography was valuable in detecting fungal vegetations.	1983	6340215
orbital aspergillosis. report of a case diagnosed by fine needle aspiration biopsy.	presentation is made of a case of orbital aspergillosis, a rare condition that may clinically mimic nonspecific orbital inflammatory disease. it can be a subacute or chronic disease that develops in otherwise healthy individuals. fine needle aspiration biopsy, which has been useful in the diagnosis of a variety of orbital diseases, provided the initial diagnosis in this case.	1983	6340402
fungal protein produced on cassava for growing rats and pigs.		1983	6341343
enzyme-linked immunosorbent assay and its application to the serological diagnosis of fungal infection.		1983	6342173
natural atmospheric microbial conditions in a typical suburban area.	ambient outdoor concentrations and size distributions of airborne microbial particles were measured approximately weekly for 2 years in a washington, d.c., suburban area. the study objective was to characterize microbial air quality in the vicinity of a proposed sewage sludge composting facility. during the study, 379 samples were taken at 17 stations, using andersen microbial samplers. concentration ranges (in viable particles per cubic meter) were as follows: airborne mesophilic fungi, 0 to 7, ...	1983	6342536
antifungal activity of enilconazole on experimental aspergillosis in chickens.	day-old chickens were infected with various inoculum sizes of airborne spores of aspergillus fumigatus, which caused high rates of mortality and morbidity. fumigation with enilconazole smoke pellets was highly successful in preventing mortality, in reducing morbidity, and in neutralizing growth inhibition of infected chickens. no side effects were observed.	1983	6342595
[3 cases of naso-sinal aspergillosis].		1983	6352216
mould counts and exacerbations of allergic bronchopulmonary aspergillosis.	the purpose of this study was to determine whether exacerbations of allergic bronchopulmonary aspergillosis (abpa) were associated with the total outdoor mould counts in the chicago area. from 1976-1980, forty-nine flares of abpa (new pulmonary infiltrate with elevation in total serum ige) occurred in nineteen patients. thirty-eight (77.5%) of flares occurred during months june through november in association with increased outdoor mould counts in chicago. this association confirms earlier obser ...	1983	6342846
a non-immunosuppressed patient with presumably invasive pulmonary aspergillosis and a favourable outcome.		1983	6343901
an atypical isolate of aspergillus fumigatus from a man affected with a pulmonary disease.		1983	6346091
a study on the possible role of chymotrypsin in the aetiology of equine chronic obstructive pulmonary disease (copd).	the chymotrypsin activity of seven batches of micropolyspora faeni and of five batches of aspergillus fumigatus culture extracts, prepared for inhalation challenge in horses, was assayed and was found to range between 0.29 and 1.45 units/mg protein and 0.02 and 0.20 units/mg protein respectively. horses affected with chronic obstructive pulmonary disease (copd) were challenged with two batches of each antigen which had different chymotrypsin activities and no significant correlations were found ...	1983	6346665
aspergillus fumigatus and other thermotolerant fungi generated by hospital building demolition.	on 13 september 1981, a 51-year-old seven-story building within our hospital complex was demolished by explosives. the concern that this event might release large numbers of thermotolerant fungi (tf), potentially hazardous to immunosuppressed patients, led us to seal hospital windows and doors. the air-handling systems were also manipulated. concentrations of airborne tf, especially aspergillus fumigatus, were determined before and after demolition, using andersen and cassella air samplers with ...	1983	6354086
[combined treatment of corneal aspergillosis using the argon laser and clotrimazole].		1983	6355634