Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| birth of twins after in vitro fertilization with flow-cytometric sorted buffalo (bubalus bubalis) sperm. | flow-cytometric sorting of mammalian sperm for production of offspring with the desired sex is one of the most important new biotechnologies available for livestock industry. the objectives of this study were: (i) to sort the sperm into x- and y-enriched populations and (ii) use the sorted sperm for in vitro fertilization (ivf) to produce sex-preselected embryo and offspring. the results revealed that the accuracy of sorted x- and y-sperm was 94% and 89%, respectively. there was a decrease in bl ... | 2007 | 17074453 |
| congenital ocular dermoid cyst in a river buffalo (bubalus bubalis) calf. | clinical and histopathological findings of a congenital ocular dermoid cyst, located at the lower eyelid of a river female buffalo (bubalus bubalis) calf were presented. a soft, fluctuant, non-tender, hyperaemic cystic mass was detected overlaying the left eye. fine needle aspirate revealed filamentous debris with no malignant cells. the cyst was treated surgically by orbital exenteration and subsequently subjected to histopathological examination. the histopathological study disclosed a conjunc ... | 2007 | 17359456 |
| isolation and sequence characterization of mammary derived growth inhibitor gene of riverine buffalo (bubalus bubalis). | in this study, attempts have been made to identify and characterize water buffalo (bubalus bubalis) mammary derived growth inhibitor (mdgi) gene, isolated from a mammary gland cdna library of lactating buffalo. the complete mdgi cdna was of 698 nucleotides, consisting 61 nucleotides in 5' utr, coding region of 402 nucleotides, and 235 nucleotides representing the 3' utr. comparison of nucleotide and deduced amino acid sequence data with that of mdgi/fatty acid binding protein (fabp) of other spe ... | 2007 | 17453652 |
| mitochondrial dna analyses of indian water buffalo support a distinct genetic origin of river and swamp buffalo. | water buffalo (bubalus bubalis) is broadly classified into river and swamp categories, but it remains disputed whether these two types were independently domesticated, or if they are the result of a single domestication event. in this study, we sequenced the mitochondrial d-loop region and cytochrome b gene of 217 and 80 buffalo respectively from eight breeds/locations in northern, north-western, central and southern india and compared our results with published mediterranean and swamp buffalo s ... | 2007 | 17459014 |
| sequence variation and molecular evolution of hormone-sensitive lipase genes in species of bovidae. | the partial sequences of exon i of hormone-sensitive lipase (hsl) genes in yak (bos grunniens), cattle (bos taurus), zebu (bos indicus), and buffalo (bubalus bubalis) were analyzed. comparisons of these sequences and the deduced amino acid sequences with the homologous hsl gene and protein sequences in other mammalian species including pig (sus scrofa), human (homo sapiens), mouse (mus musculus), and rat (rattus sp.) retrieved from the genbank were carried out and finally a phylogenetic tree was ... | 2007 | 17469775 |
| comparative pharmacokinetics of sulfamethazine after intravenous administration in bovine (bos taurus) and buffalo (bubalis bubalis) calves. | 2007 | 17472661 | |
| buffalos (bubalus bubalis) cloned by nuclear transfer of somatic cells. | cloning of buffalos (bubalus bubalis) through nuclear transfer is a potential alternative approach in genetic improvement of buffalos. however, to our knowledge, cloned offspring of buffalos derived from embryonic, fetal, or somatic cells have not yet been reported. thus, factors affecting the nuclear transfer of buffalo somatic cells were examined, and the possibility of cloning buffalos was explored in the present study. treatment of buffalo fibroblasts and granulosa cells with aphidicolin plu ... | 2007 | 17475931 |
| advanced reproductive technology in the water buffalo. | embryo transfer techniques in water buffalo were derived from those in cattle. however, the success rate is much lower in buffaloes, due to their inherent lower fertility and poor superovulatory response. the buffalo ovary has a smaller population of recruitable follicles at any given time than the ovary of the cow (89% fewer at birth). in addition, estrus detection is problematic. progress in the field of embryo transfer in water buffalo has been slow, and is primarily due to a poor response to ... | 2007 | 17481722 |
| morphological features of spermatozoa of swamp buffalo ai bulls in thailand. | the appearance and incidence of sperm abnormalities was studied in 115 ejaculates, collected periodically over 1 year covering all seasons from five mature, healthy swamp buffalo (bubalus bubalis) bulls reared under tropical conditions and serving as the current source of semen for artificial insemination (ai) in thailand. light microscopy of stained smears was used to investigate sperm head shape morphology, while unstained wet smears were used to examine other sperm abnormalities. the most com ... | 2007 | 17493162 |
| low incidence of bovine leukocyte adhesion deficiency (blad) carriers in indian cattle and buffalo breeds. | blad is an autosomal recessive genetic disease that affects holstein-friesian (hf) cattle worldwide. it is a disease characterized by a reduced expression of the adhesion molecules on neutrophils. the disease is caused by a mutation that replaces adenine at 383 with guanine, which causes an amino acid change from aspartic acid to glycine. blood samples and a few semen samples were collected from 1250 phenotypically normal individuals, including hf (n=377), hf crossbred (n=334), jersey (105), oth ... | 2007 | 17495349 |
| cloning, sequencing and expression of cdna of bovine neutrophil beta-defensin from water buffalo (bubalus bubalis). | neutrophil beta-defensins have been identified as naturally occurring potent antibacterial cationic peptides serving as effector molecules of innate immunity that provide a first line of defence against pathogens. considering the broad-spectrum antimicrobial activity against microorganisms and role in innate immunity of the neutrophil beta-defensins, it has been characterized in many livestock species including cattle, sheep, caprine and porcines. here we report the isolation, cloning, sequencin ... | 2007 | 17504507 |
| isolation and characterization of eg-like cells from chinese swamp buffalo (bubalus bubalis). | there have been few studies done on the isolation and characterization of chinese swamp buffalo embryonic germ cells (eg cells). here, we first report on eg-like cells isolated from chinese swamp buffalo fetuses. the results showed the cells grew in large, multilayered colonies, which were densely packed with an obvious border resembling mouse embryonic stem cells (es cells) and eg cells. the buffalo eg-like cells expressed ap, ssea-1, ssea-3, ssea-4 and oct-4. by rt-pcr, we found that undiffere ... | 2007 | 17532234 |
| microvascular architecture of the near-term uterine caruncles in water buffaloes (bubalus bubalis). | the present investigation was carried out on five near-term pregnant water buffaloes for studying the microvascular architecture of the uterine caruncles. the vascular casts were obtained by injection of 4:1 mixture of mercox and methylmethacrylate through the branches of the uterine arteries. after complete polymerization of the plastic, corrosion was conducted in 20% potassium hydroxide, then the vessel casts were immersed in distilled water, cut into small pieces, sputter coated with gold, an ... | 2007 | 17535357 |
| comparison of two doses of ropivacaine for lumbosacral epidural analgesia in buffalo calves (bubalus bubalis). | six male buffalo calves aged from six to eight months and weighing 100 to 120 kg were used to evaluate the efficacy and safety of caudal epidural analgesia produced by doses of 37.5 mg (5 ml) and 75 mg (10 ml) ropivacaine (0.75 per cent). the study was completed in two phases. in phase 1, all the animals were used twice to evaluate the analgesic and clinical effects of two doses of ropivacaine. in phase 2, the animals were divided into two groups of three to evaluate the effects of two doses of ... | 2007 | 17545647 |
| single-dose pharmacokinetics of marbofloxacin in egyptian buffalo (bubalus bubalis l.) steers. | the objective of this study was to investigate the pharmacokinetics of marbofloxacin (mar) following intravenous (iv) and intramuscular (im) administration of a 2.0 mg/kg body weight dosage to five healthy egyptian buffalo steers. a cross-over design was used with a washout period of 2 weeks. blood samples were obtained at 0, 5,10,15, and 20 min and at 0.5,0.75,1,2,4,6,8,10,12,24,30 and 48 hours after marbofloxacin administration. the serum marbofloxacin concentrations were quantitated using a m ... | 2007 | 17555042 |
| preliminary radiation hybrid map for river buffalo chromosome 6 and comparison to bovine chromosome 3. | we present the first radiation hybrid (rh) map of river buffalo (bubalus bubalis) chromosome 6 (bbu6) developed with a recently constructed river buffalo whole-genome rh panel (bburh(5000)). the preliminary map contains 33 cattle-derived markers, including 12 microsatellites, 19 coding genes and two ests, distributed across two linkage groups. retention frequencies for markers ranged from 14.4% to 40.0%. most of the marker orders within the linkage groups on bbu6 were consistent with the cattle ... | 2007 | 17559550 |
| phylogenetic relationships and status quo of colonies for gayal based on analysis of cytochrome b gene partial sequences. | thirty-three mutations and four different haplotypes were found when cytochrome b(cytb) gene partial sequences of 12 gayals were analyzed. together with sequences of bos indicus, bos taurus, bos grunniens and bos gaurus with bubalus bubalis as the out group, the partial sequences of cytb gene of gayals were aligned and base composition and nucleotide variation of cytb gene were analyzed. the phylogenetic trees were constructed by the nj method and the mp method respectively, both supporting almo ... | 2007 | 17560527 |
| biometry of frozen-thawed sperm from eight breeds of indian buffaloes (bubalus bubalis). | sperm morphometry, in combination with other objective traits, can be useful for developing a fertility index. the objective of the present study was to measure various biometric end points of frozen-thawed sperm from eight breeds of indian buffaloes (murrah, surti, tarai, mehsana, jaffrabadi, bhadawari, pandharpuri and nili-ravi). the sperm head of pandharpuri buffaloes had the greatest length (10.21 microm), width (6.05 microm), area (52.31 microm(2)) and perimeter (31.86 microm). the ratio of ... | 2007 | 17573105 |
| protocols for synchronizing estrus and ovulation in buffalo (bubalus bubalis): a review. | poor estrus expression and a prolonged intercalving interval compromise the reproductive efficiency of female buffaloes. these limitations are exacerbated during the hot season, when fertility decreases dramatically. pregnancy rate decrease further because difficulties in detecting estrus. to improve reproductive efficiency, several protocols of estrus and ovulation synchronization have been developed. these procedures are based on manipulating the cl, either to induce premature luteolysis using ... | 2007 | 17112577 |
| genetic variation of foot-and-mouth disease virus isolates recovered from persistently infected water buffalo (bubalus bubalis). | genetic variation of foot-and-mouth disease virus (fmdv) isolates, serotype o, recovered serially over a 1-year period from persistently infected buffalos was assessed. the persistent state was established experimentally with plaque-purified fmdv, strain o(1)campos, in five buffalos (bubalus bubalis). viral isolates collected from esophageal-pharyngeal (ep) fluids for up to 71 weeks after infection were analyzed at different times by nucleotide sequencing and t(1) rnase oligonucleotide fingerpri ... | 2007 | 17113729 |
| effects of growth hormone-releasing factor on growth hormone response, growth and feed conversion efficiency in buffalo heifers (bubalus bubalis). | the aim of this study was to determine the benefits of growth hormone-releasing factor (grf) on growth and feed conversion efficiency (fce) in buffaloes. twelve murrah buffalo heifers (bubalus bubalis) of mean age 24.8 months and mean body weight 302.4kg were divided into two groups (treatment and control) with six animals in each group. the buffaloes were given intravenous injections of bovine grf (bgrf) at a dose rate of 10microg/100kg body weight or an equal volume of saline at 15-day interva ... | 2007 | 17113797 |
| molecular characterization of coding sequences and analysis of toll-like receptor 3 mrna expression in water buffalo (bubalus bubalis) and nilgai (boselaphus tragocamelus). | toll-like receptor 3 (tlr3), an antiviral innate immunity receptor recognizes double-stranded rna, preferably of viral origin and induces type i interferon production, which causes maturation of phagocytes and subsequent release of chemical mediators from phagocytes against some viral infections. the present study has characterized tlr3 complementary dna (cdna) in buffalo (bubalus bubalis) and nilgai (boselaphus tragocamelus). tlr3 coding sequences of both buffalo and nilgai were amplified from ... | 2007 | 17119952 |
| bovine and water buffalo mx2 genes: polymorphism and antiviral activity. | millennia-long selective pressure of single-strand rna viruses on the bovine mx locus has increased the advantages of using the bovine mx protein to evaluate the ultimate significance of the antiviral role of mx proteins. the conclusions of research based only on the bovine mx1 protein showed the need for comprehensive studies that demonstrate the role of all isoforms, individually or together, especially in the presence of a second isoform, the bovine mx2 gene. this study provides information a ... | 2007 | 17119954 |
| protective effect of the nramp1 bb genotype against brucella abortus in the water buffalo (bubalus bubalis). | we tested 413 water buffalo cows (142 cases and 271 controls) for the presence of anti-brucella abortus antibodies (by the skin test, the agglutination test, and the complement fixation test) and the nramp1 genotype (by capillary electrophoresis). four alleles (nramp1a, -b, -c, and -d) were detected in the 3' untranslated region of the nramp1 gene. the bb genotype was represented among only controls, providing evidence that this genotype confers resistance to brucella abortus. the monocytes from ... | 2007 | 17145946 |
| seasonal variation in semen quality of swamp buffalo bulls (bubalus bubalis) in thailand. | to test the hypothesis that season affects the semen quality of swamp buffalo (bubalus bubalis) bulls used for artificial insemination (ai) under tropical conditions in thailand, as it does in bos taurus and bos indicus. | 2007 | 17187160 |
| giardia and cryptosporidium in water buffaloes (bubalus bubalis). | a cross-sectional survey of giardia duodenalis and cryptosporidium parvum infection in the water buffalo (bubalus bubalis) was carried out in central italy. a geographical information system (gis) was constructed utilizing as data-layers the topographic base map and the digital aerial photographs of the study area, as well as the geo-referenced points of all the buffalo farms. the survey was conducted on a sample of 90 farms, selected using a grid approach followed by proportional allocation. fo ... | 2007 | 17211662 |
| purification and some properties of galectin-1 derived from water buffalo (bubalus bubalis) brain. | an increasing number of galectins have been found in various animal species, the most abundant of which is galectin-1. the purpose of the present study was to purify and characterize galectin-1 from buffalo brain. we purified the galectin using a combination of ammonium sulphate fractionation and affinity chromatography and the homogeneity was determined by both native polyacrylamide gel electrophoresis (page) and denaturing sds-page. the molecular weight of the galectin as determined by sds-pag ... | 2007 | 17218122 |
| effects of vitrification procedures on subsequent development and ultrastructure of in vitro-matured swamp buffalo (bubalus bubalis) oocytes. | the purpose of the present study was to investigate the effects of two vitrification procedures on developmental capacity and ultrastructural changes of matured swamp buffalo oocytes. in vitro-matured oocytes were vitrified by using 35 and 40% ethylene glycol as vitrification solution for solid surface vitrification (ssv) and in-straw vitrification (isv), respectively. survival rate of vitrified-warmed oocytes, evaluated on the basis of ooplasm homogeneity, oolemma integrity and zona pellucida i ... | 2007 | 17257525 |
| milk of cow (bos taurus), buffalo (bubalus bubalis), and goat (capra hircus): a better alternative than fetal bovine serum in media for primary isolation, in vitro cultivation, and maintenance of leishmania donovani promastigotes. | tyndalized milk of goat, cow, and buffalo was found to be a potential substitute for fetal bovine serum (fbs) in the medium for the cultivation of leishmania donovani promastigotes. the numbers (means) of promastigotes reached 2.6 x 10(7), 2.3 x 10(7), and 2.1 x 10(7)/ml, respectively, in the medium supplemented with 10% milk of goat, cow, and buffalo, in comparison to 1.9 x 10(7)/ml in the control with 10% fbs. in primary isolation, the milk-supplemented medium showed that 22 out of 26 samples ... | 2007 | 17287321 |
| characterization and multilineage differentiation of embryonic stem cells derived from a buffalo parthenogenetic embryo. | embryonic stem (es) cells derived from mammalian embryos have the ability to form any terminally differentiated cell of the body. we herein describe production of parthenogenetic buffalo (bubalus bubalis) blastocysts and subsequent isolation of an es cell line. established parthenogenetic es (pges) cells exhibited diploid karyotype and high telomerase activity. pges cells showed remarkable long-term proliferative capacity providing the possibility for unlimited expansion in culture. furthermore, ... | 2007 | 17290421 |
| blood biochemical and ruminal liquor profile in buffaloes (bubalus bubalis) showing omasal impaction. | the blood biochemical and ruminal fluid parameters of 5 buffaloes showing omasal impaction were studied, together with 10 healthy buffaloes as control. the diseased buffaloes had significantly higher serum aspartate aminotransferase, bilirubin, glucose, blood urea nitrogen, creatinine, total protein, globulin and fibrinogen levels and significantly lower plasma calcium, potassium and chloride concentrations than the controls. the ruminal liquor of the diseased buffaloes revealed characteristic p ... | 2007 | 17294261 |
| genetic traceability of the geographical origin of typical italian water buffalo mozzarella cheese: a preliminary approach. | to distinguish italian protected designation of origin (pdo) water buffalo mozzarella from different producers on a molecular basis in relation to the place of manufacturing within the production district, and to develop a tool for genetic traceability of typical dairy products. | 2007 | 17309615 |
| independent maternal origin of chinese swamp buffalo (bubalus bubalis). | to obtain more knowledge on the origin and genetic diversity of the swamp buffalo (bubalus bubalis) in china, the complete mitochondrial d-loop sequences of 119 samples representing seven native types were compared. two mitochondrial dna (mtdna) lineages (lineages a and b) were determined for the chinese swamp buffalo. examination of the diversity patterns suggest that lineage a has undergone a population expansion event. divergence of lineages a and b was estimated at 18,000 years ago. combined ... | 2007 | 17313588 |
| differentiating human bone from animal bone: a review of histological methods. | this review brings together a complex and extensive literature to address the question of whether it is possible to distinguish human from nonhuman bone using the histological appearance of cortical bone. the mammalian species included are rat, hare, badger, racoon dog, cat, dog, pig, cow, goat, sheep, deer, horse, water buffalo, bear, nonhuman primates, and human and are therefore not exhaustive, but cover those mammals that may contribute to a north american or eurasian forensic assemblage. th ... | 2007 | 17316219 |
| the nramp1aa genotype confers susceptibility to brucella abortus in water buffalo. | the 3' untranslated region of the water buffalo nramp1 (natural resistance-associated macrophage protein 1) gene contains two alleles (nramp1a and nramp1b), as detected by the denaturing gradient gel electrophoresis (dgge) technique. the nramp1bb genotype is associated with resistance of water buffalo to the intracellular pathogen brucella abortus. this article provides evidence that the nramp1aa genotype is associated with susceptibility to the same pathogen. susceptibility or resistance of wat ... | 2007 | 17334658 |
| qualitative and quantitative studies of the inferior olivary complex in the water buffalo (buballus bubalis). | the shape and neuronal number of the inferior olivary complex (ioc) were determined in the water buffalo (buballus bubalis). the configuration and interrelations of the ioc compartments were ascertained by investigating serial sections through the whole rostro-caudal extent of the ioc. nissl-stained celloidin sections of six water buffalo's brainstems were used. the ioc in the water buffalo consisted of three major nuclei and four small cell groups. the medial accessory olivary nucleus (mao) had ... | 2007 | 17339758 |
| development of a competitive quantitative pcr strategy for evaluating the expression stability of 18s rrna during in vitro maturation of buffalo (bubalus bubalis) follicular oocytes. | the present work describes the development of a quantitative competitive pcr strategy for quantifying the relative abundance of 18s rrna transcripts in buffalo oocytes during in vitro maturation (ivm). as a method, the competitive pcr overcomes some of the shortcomings of conventional reverse transcriptase polymerase chain reaction (rt-pcr) procedure making it a more authentic quantitative method. a composite primer based approach was used to generate the competitor cdna to be used as external c ... | 2007 | 17348978 |
| seroepidemiology of neospora caninum and toxoplasma gondii in cattle and water buffaloes (bubalus bubalis) in the people's republic of china. | a seroepidemiological survey of neospora caninum and toxoplasma gondii in cattle and water buffaloes was carried out in the people's republic of china. serum samples were obtained from dairy (n=262, 9 herds in 9 provinces) and beef cattle (n=10, 1 herd) and water buffaloes (n=40) in china. all sera were tested for antibodies to n. caninum and t. gondii by an enzyme-linked immunosorbent assay (elisa) and an indirect agglutination test (iat), respectively. the overall seroprevalence of n. caninum ... | 2007 | 17010521 |
| vegf system expression in different stages of estrous cycle in the corpus luteum of non-treated and superovulated water buffalo. | water buffaloes are easily adaptable animals, whose raising and economical exploitation have been growing in the last three decades all over the world. hyperstimulation of ovarian function in this species is a common technique aiming to improve reproductive performance. superovulatory treatment affects corpus luteum (cl) function, which is highly correlated to angiogenic process. the aim of this study was therefore to assess the temporal protein and mrna expression of vegf and its receptors in t ... | 2007 | 17014980 |
| isolation and characterization of embryonic stem cell-like cells from in vitro-produced buffalo (bubalus bubalis) embryos. | this study was carried out to isolate and characterize buffalo embryonic stem (es) cell-like cells from in vitro-produced embryos. inner cell mass (icm) cells were isolated either mechanically or by enzymatic digestion from 120 blastocysts whereas 28 morulae were used for the isolation of blastomeres mechanically. the icm cells/ blastomeres were cultured on mitomycin-c-treated feeder layer. primary cell colony formation was higher (p < 0.05) for hatched blastocysts (73.1%, 30/41) than that for e ... | 2007 | 17034054 |
| cryopreservation of in vitro matured buffalo (bubalus bubalis) oocytes by minimum volumes vitrification methods. | the aim of this study was to evaluate the efficiency of the solid surface vitrification (ssv) and the cryoloop vitrification (clv) methods to cryopreserve in vitro matured buffalo oocytes. another objective of the work was to investigate whether the presence of cumulus cells affects the efficiency of oocyte vitrification in this species. in the ssv method, oocytes were vitrified in a solution of 35% ethylene glycol, 5% polyvinyl-pyrrolidone and 0.4% trehalose and they were warmed in a 0.3m treha ... | 2007 | 16698202 |
| ultrastructural and morphometric characterization of buffalo (bubalus bubalis) ovarian preantral follicles. | the main objective of the present study was to characterize buffalo preantral ovarian follicles. parts of ovarian cortex, collected from postpubertal buffalo females that were having estrous cycles at regular intervals, were selected under stereomicroscopy and processed for optic and transmission electron microscopy. primordial follicles were characterized as an oocyte encircled by one layer of flattened cells. the buffalo primordial follicle has a mean diameter of 35 microm and the oocyte diame ... | 2007 | 16580155 |
| tyrosine phosphorylation of a 38-kda capacitation-associated buffalo (bubalus bubalis) sperm protein is induced by l-arginine and regulated through a camp/pka-independent pathway. | the aim of the present study was to determine the effect of l-arginine on nitric oxide (no*) synthesis, capacitation and protein tyrosine phosphorylation in buffalo spermatozoa. ejaculated buffalo spermatozoa were capacitated in the absence or presence of heparin, or l-arginine or n(omega)-nitro-l-arginine methyl ester (l-name), an inhibitor of nitric oxide synthase (nos) for 6 h. capacitating spermatozoa generated no* both spontaneously and following stimulation with l-arginine and l-name quenc ... | 2008 | 17355240 |
| production of superoxide anion and hydrogen peroxide by capacitating buffalo (bubalus bubalis) spermatozoa. | in the present study attempts were made to detect and quantify the generation of superoxide anion (o(2)(*-)) and hydrogen peroxide (h(2)o(2)) by capacitating buffalo spermatozoa. ejaculated buffalo spermatozoa were suspended in sp-talp medium at 50x10(6)ml(-1) and incubated at 38.5 degrees c with 5% co(2) in air in the absence or presence of heparin (a capacitation inducer) or reduced nicotinamide adenine dinucleotide phosphate (nadph) or diphenyleneiodonium (dpi, a flavoprotein inhibitor) for 6 ... | 2008 | 17270367 |
| evaluation of sperm functional attributes in relation to in vitro sperm-zona pellucida binding ability and cleavage rate in assessing frozen thawed buffalo (bubalus bubalis) semen quality. | the objective of this study was to evaluate sperm functional attributes in relation to in vitro sperm-zona binding ability and cleavage rate in assessing frozen thawed buffalo (bubalus bubalis) semen quality. frozen-thawed forty-eight ejaculates from eight surti buffalo bulls (six ejaculates/bull) obtained by artificial vagina were used. frozen semen from each bull was thawed, pooled, and subjected for sperm functional (six replicates) and in vitro fertilization (four replicates) tests. the prog ... | 2008 | 17576042 |
| expression stability of two housekeeping genes (18s rrna and g3pdh) during in vitro maturation of follicular oocytes in buffalo (bubalus bubalis). | the present study was undertaken to evaluate the expression stability of two housekeeping genes (hkgs), 18s rrna and g3pdh during in vitro maturation (ivm) of oocytes in buffalo, which qualifies their use as internal controls for valid qrt-pcr estimation of other oocyte transcripts. a semi quantitative rt-pcr system was used with optimised qrt-pcr parameters at exponential pcr cycle for evaluation of temporal expression pattern of these genes over 24 h of ivm. 18s rrna was found more stable in i ... | 2008 | 17537596 |
| ultrasonographic fetometry and determination of fetal sex in buffaloes (bubalus bubalis). | the aim of the current study was to establish ultrasonic biometric threshold of different fetal parts in buffaloes and to evaluate the feasibility and accuracy of ultrasonic fetal sex determination. serial ultrasonographic examinations were carried out on twelve pregnant buffalo-cows, during which fetal parts were measured, and fetal sex and presentation were determined. the obtained results revealed that embryo and amniotic vesicle (av) were detected by the forth and fifth week of pregnancy, re ... | 2008 | 17544605 |
| 2,3,7,8-tetrachlorodibenzo-p-dioxin increases bovine herpesvirus type-1 (bhv-1) replication in madin-darby bovine kidney (mdbk) cells in vitro. | dioxin-2,3,7,8-tetrachlorodibenzo-p-dioxin (tcdd) is a common environmental toxin of current interest. in the last years, higher levels of tcdd than those permitted in ue [european commission. 2002. european commission recommendation 2002/201/ce. official gazette, l 67/69] were detected in milk samples from cow, water buffalo, goat, and sheep raised on some areas of campania region (south italy). dioxin often causes immunosuppression and might render the animal liable to viral infections. in add ... | 2008 | 17516555 |
| influence of the duration of in vitro maturation and gamete co-incubation on the efficiency of in vitro embryo development in italian mediterranean buffalo (bubalus bubalis). | the aim of this study was to investigate the effect of the duration of oocyte in vitro maturation (ivm) and gamete co-incubation on the in vitro embryo (ivep) production efficiency in river buffalo. in experiment 1, abattoir-derived cumulus oocyte complexes were fixed at 0, 3, 6, 9, 12, 15, 18, 21 and 24 h after the start of in vitro maturation to study the kinetics of nuclear maturation. in experiment 2, cumulus oocyte complexes were fertilized in vitro following in vitro maturation for 18, 21, ... | 2008 | 17481834 |
| effect of slow freezing on morphology and developmental competence of buffalo (bubalus bubalis) immature oocytes. | the present study was conducted to evaluate the effects of three cryoprotectants, dimethyl sulphoxide (dmso), ethylene glycol (eg) and 1,2-propanediol (proh), each used at two concentrations (1.0 and 1.5 m) on the morphology, maturation rate and developmental capacity of usable quality immature buffalo oocytes subjected to slow freezing. the addition of the cryoprotectant before freezing and its dilution after thawing were carried out in a two- (for 1.0 m) or three-step manner (for 1.5 m). the i ... | 2008 | 17449204 |
| expression of cytochrome p450 aromatase transcripts in buffalo (bubalus bubalis)-ejaculated spermatozoa and its relationship with sperm motility. | the cytochrome p450 aromatase (aromp450) deficient mice are infertile due to an impairment of spermatogenesis associated with a decrease in sperm motility and inability to fertilize oocytes. the sperm analysis showed decreased sperm motility in humans, having cyp19 gene mutations. further, in human, it was hypothesized that aromatase could be used as marker of sperm quality, particularly in the acquisition of its motility. however, there is no information regarding the expression of aromp450 in ... | 2008 | 17851018 |
| molecular characterization of cdna encoding b. taurus cathelicidin-7 like antibiotic peptide from bone marrow cells of bubalus bubalis. | cathelicidins represent a diverse family of endogenous cationic antibiotic peptide present in all mammalian species. in the present study, a novel cathelicidin cdna was identified and characterized from bone marrow cells of buffalo (bubalus bubalis) using rt-pcr based approach. the cdna encodes a propeptide of 1.18 kda with net positive charge at neutral ph. the precursor peptide possesses a signal peptide of 29 amino acids and a biologically active peptide of 34 residues. comparison of sequence ... | 2008 | 17852341 |
| physicochemical properties and oxidative inactivation of soluble lectin from water buffalo (bubalus bubalis) brain. | lectins are carbohydrate-binding proteins present in a wide variety of plants and animals, which serve various important physiological functions. a soluble beta-galactoside binding lectin has been isolated and purified to homogeneity from buffalo brain using ammonium sulphate precipitation (40-70%) and gel permeation chromatography on sephadex g50-80 column. the molecular weight of buffalo brain lectin (bbl) as determined by sds-page under reducing and non-reducing conditions was 14.2 kda, howev ... | 2008 | 17763948 |
| characterization of the luteinizing hormone beta (lh-beta) subunit gene in the indian river buffalo (bubalus bubalis). | the cdna sequence of leuteinizing hormone (lh) beta subunit was determined to understand the molecular basis for silent oestrus behavior and poor response to superovulation in buffalo. the lh-beta cdna contains an open reading frame 426 bp long. the deduced sequence of the lh-beta is 141 amino acids in length. the amino acid sequences of the indian river buffalo lh-beta subunit showed overall similarity to those of other mammals. the nucleotide sequence variability of lh-beta was studied in more ... | 2008 | 17485093 |
| in vitro embryo development and blastocyst hatching rates following vitrification of river buffalo embryos produced from oocytes recovered from slaughterhouse ovaries or live animals by ovum pick-up. | the present study was undertaken to determine whether the source of oocytes (ovum pick up versus slaughterhouse ovaries) affected in vitro embryo production and embryo survival (as measured by blastocyst hatching rates) following vitrification in buffaloes (bubalus bubalis). oocytes recovered from live buffaloes (n=6) by ovum pick up (opu) and by manual aspiration from slaughterhouse ovaries were in vitro matured, fertilized and cultured to blastocyst stage under same culture conditions. vitrifi ... | 2008 | 17689038 |
| duck egg yolk in extender improves the freezability of buffalo bull spermatozoa. | we investigated the use of duck egg yolk (dey), guinea fowl egg yolk (gfey) and indian indigenous hen (desi) egg yolk (idey) in extender for improving the post-thaw quality of buffalo (bubalus bubalis) bull spermatozoa, and compared it with commercial hen egg yolk (chey; control). for this purpose, two consecutive ejaculates of semen from each of two nili-ravi buffalo bulls were collected on 1 day each week for 5 weeks (replicates; n=5) with artificial vagina (42 degrees c). split pooled ejacula ... | 2008 | 17709214 |
| effect of reactive oxygen species on capacitation and associated protein tyrosine phosphorylation in buffalo (bubalus bubalis) spermatozoa. | in the present study, the effect of two particular reactive oxygen species (ros), superoxide anion (o(2)(-)) and hydrogen peroxide (h(2)o(2)) on buffalo (bubalus bubalis) sperm capacitation and associated protein tyrosine phosphorylation was studied. ejaculated buffalo spermatozoa were suspended in sp-talp medium at 50 x 10(6)/ml and incubated at 38.5 degrees c for 6h with or without heparin (10(g/ml; a positive control), or xanthine (x; 0.5mm)-xanthine oxidase (xo; 0.05 u/ml)-catalase (c; 2100 ... | 2008 | 17646064 |
| effect of selenium and copper supplementation on blood metabolic profile in male buffalo (bubalus bubalis) calves. | twenty male buffalo calves (8-9 months, 112.1 +/- 7.69 kg) were divided into four groups of five animals in each and fed diets without (t1) or supplemented with 0.3 ppm selenium (se) (t2), 0.3 ppm se + 10 ppm copper (cu) (t3), and 10 ppm cu (t4) for 120 days during which blood samples were collected on day 0, 40, 80, and 120. concentrations of glucose, total protein, urea, uric acid, and creatinine were similar in all the four groups, but the level of globulin was significantly (p < 0.01) increa ... | 2008 | 18185997 |
| multi-host transmission dynamics of schistosoma japonicum in samar province, the philippines. | among the 6.7 million people living in areas of the philippines where infection with schistosoma japonicum is considered endemic, even within small geographical areas levels of infection vary considerably. in general, the ecological drivers of this variability are not well described. unlike other schistosomes, s. japonicum is known to infect several mammalian hosts. however, the relative contribution of different hosts to the transmission cycle is not well understood. here, we characterize the t ... | 2008 | 18215106 |
| a novel method for species identification in milk and milk-based products. | this study describes a method for species-specific detection of animal dna from different species (cattle, sheep, goat, water buffalo) in milk and dairy products. a primer set was designed in conserved region on the basis of the alignment of the sequence codifying the genomic kappa-casein gene in order to amplify all four species with a single primer pair. polymorphisms were detected via minisequencing with extension primers designed in conserved sequences for haplotype determination that allow ... | 2008 | 18226307 |
| a survey of abortifacient infectious agents in livestock in luzon, the philippines, with emphasis on the situation in a cattle herd with abortion problems. | in the philippines, insufficient consideration has been given to the implementation of systematic control measures against major abortifacient infectious agents in livestock. to elucidate the epidemiology of abortifacient infectious agents in livestock, the prevalence of four abortifacient agents was assessed. initially, a total of 96 cattle including 17 cows with history of abortion were examined in a herd in luzon at the request of the farm owner. six (35.3%) of the 17 aborting cows were found ... | 2008 | 18243149 |
| [molecular phylogeny of the gayal inferred from the analysis of cytochrome b gene entire sequences]. | the gayal (bos frontalis) is a very rare, semi-wild and semi-domestic bovine species. there still exist remarkable divergences on the gayal's origin and phylogenetic status. the cytochrome b (cyt b) gene entire sequences (1,140 bp) of 11 gayals were sequenced and analyzed. combined with other bovine cyt b entire sequences cited in genbank, the phylogenetic trees of genus bos were reconstructed by neighbor-joining (nj) and maximum parsimony (mp) methods with bubalus bubalis as outgroup. sequence ... | 2008 | 18244904 |
| a radiation hybrid map of river buffalo (bubalus bubalis) chromosome 7 and comparative mapping to the cattle and human genomes. | a preliminary radiation hybrid (rh) map containing 50 loci on chromosome 7 of the domestic river buffalo bubalus bubalis (bbu; 2n = 50) was constructed based on a comparative mapping approach. the rh map of bbu7 includes thirty-seven gene markers and thirteen microsatellites. all loci have been previously assigned to bos taurus (bta) chromosome bta6, which is known for its association with several economically important milk production traits in cattle. the map consists of two linkage groups spa ... | 2008 | 18253035 |
| comparative fish-mapping of twelve loci in river buffalo and sheep chromosomes: comparison with hsa8p and hsa4q. | twelve loci (11 of type i and 1 of type ii) previously fish-mapped in cattle were comparatively fish-mapped in both river buffalo chromosome 1p (bbu1p) and homologous chromosome 26 of sheep (oar26), extending the cytogenetic maps in both chromosome species and providing a more precise localization of these loci in single chromosome bands than previous locations on bta27. bovine bac clones containing dctd, c4orf20, casp3, tlr3, msr1, fat, lonrf1, dlc1, c8orf41, cssm036, lsm1 and eif4ebp1 were use ... | 2008 | 18253036 |
| cysteamine supplementation of in vitro maturation medium, in vitro culture medium or both media promotes in vitro development of buffalo (bubalus bubalis) embryos. | the effects of supplementation of in vitro maturation (ivm) or in vitro culture (ivc) or both ivm and ivc media with cysteamine on the yield, hatching rate (hr) and total cell number (tcn) of buffalo blastocysts were examined. oocytes obtained from slaughterhouse buffalo ovaries were subjected to ivm and ivf. the ivm or ivc media were supplemented with 0, 50, 100 or 200 microm cysteamine. supplementation of ivm medium with 50 microm cysteamine increased (p < 0.01) the cleavage rate and blastocys ... | 2008 | 18255014 |
| comparative therapeutic effect of toltrazuril, sulphadimidine and amprolium on eimeria bovis and eimeria zuernii given at different times following infection in buffalo calves (bubalus bubalis). | we compared the therapeutic effect of three anticoccidial drugs (toltrazuril, sulphadimidine and amprolium) in buffalo (bubalus bubalis) calves experimentally infected with eimeria bovis (e. bovis) and e. zuernii oocysts (3 x 104oocyst/calf). buffalo calves (1.5-4 month old, 70-kg body weight) were randomly allocated into 3 groups (9 calves each). group t was experimentally infected with oocysts and treated with toltrazuril (20 mg/kg bw twice orally at a 1-week interval). group s was experimenta ... | 2008 | 18262668 |
| development of a pronuclear dna microinjection technique for production of green fluorescent protein-expressing bubaline (bubalus bubalis) embryos. | oocytes from abattoir-derived bubaline (bubalus bubalis) ovaries were subjected to ivm and ivf; the objective was to develop a pronuclear dna microinjection technique to produce embryos expressing green fluorescent protein (gfp). the largest proportion (61.2%) of zygotes in which one (1 pn) or two pronuclei (2 pn) were visible was when centrifugation (14,000 x g for 15 min) was done 16 h after insemination. centrifugation had no adverse effects on cleavage rate, development to morulae/blastocyst ... | 2008 | 18272213 |
| oocyte recovery by ovum pick up and embryo production in river buffaloes (bubalus bubalis). | ovum pick up (opu) was conducted twice a week for 12 weeks in six cycling, non-descriptive (local breed), indian buffaloes to study the efficiency of opu on recovery of oocytes for embryo production. opu was performed using an ultrasound equipment with a 5-mhz transvaginal transducer, a single-lumen, 18-gauge, 55-cm-long needle and a constant vacuum pressure of 110 mmhg. the number and size of follicles were determined before puncture. the recovered oocytes were graded, washed, matured for 24 h ... | 2008 | 18282214 |
| follicular fluid concentrations of glucose, lactate and pyruvate in buffalo and sheep, and their effects on cultured oocytes, granulosa and cumulus cells. | the objective was to determine ovarian follicular fluid concentrations of glucose, lactate, and pyruvate in relation to follicle size in buffalo and sheep. the effect of varying concentrations of these substances on in vitro oocyte maturation, oocyte protein content, and granulosa and cumulus cell growth was also investigated. follicular fluid was aspirated from various sizes of follicles (from ovaries without a dominant follicle) collected from adult, cycling nonpregnant buffalo (bubalus bubali ... | 2008 | 17950822 |
| detection of bovine viral diarrhoea virus (bvdv) nucleic acid and antigen in different organs of water buffaloes (bubalus bubalis). | bovine viral diarrhoea virus (bvdv) is a pestivirus that infects mainly bovine cattle. nevertheless, there are several reports about infections in other members of the artiodactyla order including serological studies, that indicate infection of bvdv in buffaloes. the aim of this article is to study the presence of bvdv in three young water buffaloes, displaying nonspecific clinical signs, compatible with the bvdv infection. both immunohistochemistry and rt-pcr confirmed the presence of bvdv in t ... | 2008 | 17949764 |
| biological and genetic analysis of a bovine-like coronavirus isolated from water buffalo (bubalus bubalis) calves. | we describe the isolation, biological and genetic characterization of a host-range variant of bovine coronavirus (bcov) detected in water buffalo (bubalus bubalis). by conventional and real-time rt-pcr assays, the virus was demonstrated in the intestinal contents of two 20-day-old buffalo calves dead of a severe form of enteritis and in the feces of additional 17 buffalo calves with diarrhea. virus isolation, hemagglutination and receptor-destroying enzyme activity showed that the buffalo corona ... | 2008 | 17916374 |
| pathogenic 'bison-type' mycobacterium avium subspecies paratuberculosis genotype characterized from riverine buffalo (bubalus bubalis) in north india. | despite low per-animal productivity of ruminants in developing countries, johne's disease has not been investigated in buffaloes, which are primarily found in these countries. this is due to lack of expertise, diagnostic kits and priority to production diseases like johne's disease. presence of pathogenic mycobacterium avium subspecies paratuberculosis (map) was investigated by screening of target tissues (mesenteric lymph nodes and large intestine) by culture and is 900 pcr, in 50 sacrificed bu ... | 2008 | 17804069 |
| mannose-binding lectin haplotypes influence brucella abortus infection in the water buffalo (bubalus bubalis). | a case-control study established that the haplotype pair hya/hya at the mbl (mannose binding lectin) locus of water buffalo is associated with resistance to brucella abortus infection (p < 10(-7)) and the haplotype pairs lyd/lyd with susceptibility to the same pathogen (p < 10(-7)). the subjects included in the present study were tested twice-at a 1-month interval-for the presence of anti-b. abortus antibodies in the serum by agglutination, complement fixation and flow cytometry. cases (335 subj ... | 2008 | 18330558 |
| in vitro embryo production in buffalo (bubalus bubalis) using sexed sperm and oocytes from ovum pick up. | the objective was to explore the use of sexed sperm and opu-derived oocytes in an ivp system to produce sex-preselected bubaline embryos. oocytes were recovered from 20 fertile murrah and nili-ravi buffalo cows by repeated (twice weekly) ultrasound-guided transvaginal ovum pick up (opu), or by aspiration of abbatoir-derived bubaline ovaries, and subjected to ivf, using frozen-thawed sexed or unsexed bubaline semen. on average, 4.6 oocytes were retrieved per buffalo per session (70.9% were grades ... | 2008 | 18336893 |
| genetic relationship and diversity analysis of indian water buffalo (bubalus bubalis). | the water buffalo (bubalus bubalis) is an important dairy animal on the indian subcontinent and in southeast asian countries. the diversity and differentiation among 12 populations or breeds of buffalo were studied. data were generated and analyzed from 527 animals belonging to 10 recognized breeds and 2 additional populations of indian buffalo by using 22 microsatellite loci. relationships among buffalo breeds and populations were estimated based on genetic distances. the bayesian analysis grou ... | 2008 | 18344309 |
| water buffalo mozzarella cheese stored in polysaccharide-based gels: correlation between prolongation of the shelf-life and physicochemical parameters. | an innovative packaging system has been developed, based on natural gels, that has shown the peculiar characteristic to strongly increase the shelf life of water buffalo mozzarella cheese. to explain the mechanism of action of the gel, measurements of ca and na in the cheese and in the storage liquid were carried out, together with ph determination. a correlation has been found between the constant level of ca and ph in the cheese and the prolongation of nutritional characteristics; in fact, bot ... | 2008 | 18349224 |
| organization and differential expression of the gaca/gata tagged somatic and spermatozoal transcriptomes in buffalo bubalus bubalis. | simple sequence repeats (ssrs) of gaca/gata have been implicated with differentiation of sex-chromosomes and speciation. however, the organization of these repeats within genomes and transcriptomes, even in the best characterized organisms including human, remains unclear. the main objective of this study was to explore the buffalo transcriptome for its association with gaca/gata repeats, and study the structural organization and differential expression of the gaca/gata repeat tagged transcripts ... | 2008 | 18366692 |
| epizootic abortion related to infections by chlamydophila abortus and chlamydophila pecorum in water buffalo (bubalus bubalis). | water buffalo (bubalus bubalis) are affected by high rates of embryonic mortality and abortion related to infectious diseases and non-infectious factors. a number of viral and bacterial infections have been associated with reproductive failure, but there is limited information on the role of chlamydial infections. in order to investigate the presence and the role of chlamydiaceae in water buffalo a retrospective study was performed in a herd with a history of reproductive failure. during an 11-m ... | 2008 | 18374406 |
| peptidomic approach based on combined capillary isoelectric focusing and mass spectrometry for the characterization of the plasmin primary products from bovine and water buffalo beta-casein. | the main peptides produced by hydrolysis of water buffalo beta-casein with plasmin were characterized by capillary electrophoresis and mass spectrometry and compared with their bovine homologous. a novel breakdown product arising from the hydrolysis of water buffalo beta-casein, originated by the presence of a plasmin-sensitive lys bond at position 68 was identified, which was not present in bovine beta-casein. on the basis of this evidence, an improved procedure for the detection and the differ ... | 2008 | 18400224 |
| acute coliform mastitis in buffaloes (bubalus bubalis): clinical findings and treatment outcomes. | this report was delineated to study the clinical, bacteriological and therapeutic aspects concerned with acute coliform mastitis in buffaloes. bacteriological examination of 80 quarter milk samples obtained aseptically from 56 buffaloes with acute mastitis revealed that coliform bacteria was the most common pathogen (45 cases) followed by staphylococcus aureus (seven cases) then streptococcus uberis (three cases), and streptococcus agalactiae (one case). clinically, hotness, swelling and painful ... | 2008 | 18422251 |
| ameliorative effects of boron on serum profile in buffalo (bubalus bubalis) fed high fluoride ration. | an experiment was undertaken to evaluate the protective role of boron on the serum profile of buffalo calves fed a high fluoride ration. twelve male murrah buffalo (bubalus bubalis) calves of 6-8 months age, divided into three groups of four calves in each, were fed basal diets and supplemented with sodium fluoride (naf, 60 ppm) alone or in combination with borax (na2b4o7.10h2o, 140 ppm) for 90 days. boron (b) was added in the ration as borax to make @140 ppm boron (elemental b) on dm basis in t ... | 2008 | 18422253 |
| first radiation hybrid map of the river buffalo x chromosome (bbux) and comparison with btax. | we report the first radiation hybrid map of the river buffalo x chromosome generated from a recently constructed river buffalo (bubalus bubalis) whole-genome radiation hybrid panel (bburh(5000)). this map contains a total of 33 cattle-derived markers, including 10 genes, four ests and 19 microsatellites. the markers are distributed in two linkage groups: lg1 contains eight markers spanning 125.6 cr, and lg2 contains 25 markers spanning 366.3 cr. lg1 contains six markers in common with bovine seq ... | 2008 | 18307583 |
| isolation of new pregnancy-associated glycoproteins from water buffalo (bubalus bubalis) placenta by vicia villosa affinity chromatography. | the present study describes the isolation and characterization of new pregnancy-associated glycoprotein molecules (pag) from midpregnancy and late-pregnancy placentas in the water buffalo (bubalus bubalis). after extraction, the homogenates are subjected to acid and ammonium sulfate precipitations followed by deae chromatography. subsequently, the water buffalo pag (wbpag) from these solutions are enriched by vicia villosa agarose (vva) affinity chromatography. as determined by western blotting ... | 2008 | 18308351 |
| diagnostic and prognostic indicators of omasal impaction in buffaloes (bubalus bubalis). | twelve of 46 female buffaloes with abdominal disorders were diagnosed with omasal impaction. they had been fed finely chopped machine-prepared straw. they were characterised by anorexia, an absence of defecation, abdominal distension, ruminal hypomotility or atony and a suspension of rumination. omasal impaction was confirmed upon left flank laparorumenotomy on the basis of the size of the omasum and the consistency of its contents. after ruminal evacuation, a long flexible pipe was introduced t ... | 2008 | 18310560 |
| molecular characterization of toll-like receptor 2 of ruminants and comparative expression in nilgai (boselaphus tragocamelus) vs. buffalo (bubalus bubalis). | toll-like receptors play role in the innate immune responses and orchestrate the adaptive immunity by induction of proinflammatory cytokines and up-regulation of costimulatory molecules. the present study has characterized tlr2 cdna in nilgai, buffalo, sheep and goat that recognizes the peptidoglycan of gram-positive bacteria. tlr2 coding sequences were amplified from monocytes cdna and cloned in pgemt-easy vector for nucleotide sequencing. sequence analysis revealed 2355-bp-long tlr2 open readi ... | 2008 | 18312593 |
| effect of antibiotics in extender on bacterial and spermatozoal quality of cooled buffalo (bubalus bubalis) bull semen. | the present study was designed to study the effect of traditional antibiotic combination (streptomycin and penicillin; sp) and relatively modern combination of antibiotics (gentamycin, tylosin, lincomycin and spectinomycin; gtls) in extender on bacterial control and spermatozoal quality of liquid buffalo bull semen stored at 5 degrees c. semen collected from nili-ravi buffalo bulls (n = 10) was diluted with skim milk extender containing either sp (streptomycin 1000 microg/ml and penicillin 1000 ... | 2008 | 18042206 |
| association of microsatellite (gt)n polymorphism at 3'utr of nramp1 with the macrophage function following challenge with brucella lps in buffalo (bubalus bubalis). | brucella abortus is a facultative intracellular pathogen that survives and replicates in host macrophages. hence, macrophage function plays an important role in influencing natural resistance/susceptibility to intracellular pathogen. the natural resistance associated macrophage protein 1 (nramp1; erstwhile referred as ity/lsh/bcg), a transmembrane protein, regulates activity of macrophages against intracellular pathogens. in bovine, natural resistance to brucellosis is significantly associated w ... | 2008 | 18078724 |
| effect of type of cryoprotectant on morphology and developmental competence of in vitro-matured buffalo (bubalus bubalis) oocytes subjected to slow freezing or vitrification. | the present study examined the effects of different cryoprotectants on morphology and developmental competence of in vitro-matured buffalo oocytes after slow freezing or vitrification. after slow freezing in dimethyl sulfoxide (dmso), ethylene glycol (eg) or 1,2-propanediol (proh), at 1.0 or 1.5 m each, the proportion of morphologically normal oocytes recovered was significantly higher (p < 0.05) with 1.5 than 1.0 m for all cryoprotectants and was highest (p < 0.05) for 1.5 m dmso. following vit ... | 2008 | 18462611 |
| sex chromosome abnormalities and sterility in river buffalo. | thirteen male river buffaloes, 119 females with reproductive problems (which had reached reproductive age but had failed to become pregnant in the presence of bulls) and two male co-twins underwent both clinical and cytogenetic investigation. clinical analyses performed by veterinary practitioners revealed normal body conformation and external genitalia for most females. however, some subjects showed some slight male traits such as large base horn circumference, prominent withers and tight pelvi ... | 2008 | 18467836 |
| chromosomal expression and localization of aphidicolin-induced fragile sites in the standard karyotype of river buffalo (bubalus bubalis). | the present study reports on the chromosomal expression and localization of aphidicolin-induced fragile sites in the standard karyotype of river buffalo (bubalus bubalis, 2n = 50) with the aim of establishing a 'fragile site map' of the species. totally, 400 aphidicolin-induced breakages were analyzed from eight young and clinically healthy animals, four males and four females; these breakages were localized in 106 rbg-negative chromosome bands or at the band-interband regions. the number of bre ... | 2008 | 18467845 |
| to catch a buffalo: field immobilisation of asian swamp buffalo using etorphine and xylazine. | to demonstrate the efficacy of a mixture of etorphine and xylazine to safely immobilise wild buffalo (bubalus bubalis) in the field. | 2008 | 18498561 |
| restricted genetic diversity in the ubiquitous cattle parasite, sarcocystis cruzi. | although parasites of the genus sarcocystis have likely cycled between bovine herbivores and canine carnivores for tens of millions of years, humans may have profoundly influenced the ecology and evolution of those prevalent in domesticated dogs and cattle. to preliminarily assess the possibility of such anthropogenic effects, we surveyed genetic variation in conserved (18s small subunit) and variable (its-1) portions of ribosomal dna from a large sample of sarcocystis cruzi occurring in taurine ... | 2008 | 18501682 |
| echinococcus ortleppi and e. granulosus g1, g2 and g3 genotypes in italian bovines. | to increase the knowledge on echinococcus genotypes infesting cattle and water buffaloes (bubalus bubalis) born and bred in italy, the germinal layer of hydatid cysts was collected from the liver and the lungs of 80 animals slaughtered in 2007. two mitochondrial genes (the cytochrome c oxidase subunit i and the nadh subunit i) were tested by pcr. four genotypes were identified: g1 (sheep strain), g2 (tasmanian sheep strain), g3 (buffalo strain), and g5 (cattle strain). fertile cysts were detecte ... | 2008 | 18514422 |
| dna-based vaccines protect against zoonotic schistosomiasis in water buffalo. | schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in china where water buffaloes account for approximately 75% of disease transmission. interventions that reduce schistosome infection in water buffaloes will enhance their health simultaneously reducing disease transmission to humans. while chemotherapy has proved successful, it requires continued time consuming and expensive mass treatments. a more sustainable option would be development of vaccines that ... | 2008 | 18524429 |
| assessment of water buffalo health and productivity in a communal management system in the philippines. | this study aimed to generate a profile of the health and productivity of water buffaloes in a communal setting. using the epi-info version 6.04 for data management, a coded information system was used to accommodate data coming from the reference population. calves and cows that were born and milked, respectively, were enrolled and monitored for six months. the key outcomes of interest monitored in this study included mortality, morbidity and productivity. results of the study showed a 93.7 perc ... | 2008 | 18551780 |
| relationship between peripheral plasma inhibin and fsh concentrations in sahiwal cows (bos indicus) and murrah buffaloes (bubalus bubalis) during estrous cycle. | the present investigation was undertaken to study the relationship between circulating inhibin and fsh concentrations during the estrous cycle in buffaloes and sahiwal cattle. the pattern of inhibin concentrations was similar, with peak concentrations on day -2 (day 0 = day of estrus) and minimum concentrations on days 12 and 11 in buffaloes and cattle, respectively. circulating fsh concentrations were the highest on day 0 and lowest on days 8 and 13 in buffaloes and cattle, respectively. periph ... | 2008 | 18575966 |
| cryptosporidiosis in buffalo calves (bubalus bubalis): prevalence and potential risk factors. | the objective of the present study was to describe the prevalence and risk factors associated with cryptosporidiosis in buffalo calves in middle egypt. during one year, 458 fecal samples were collected from buffalo calves less than 3 month age in 55 small scale herds and examined for the presence of cryptosporidium oocysts. data describing age, gender, season, and herd management practices were gathered to assess potential risk factors. fecal examination showed that 14.19% of the examined calves ... | 2008 | 18575969 |
| fatal intestinal coccidiosis in a three week old buffalo calf (bubalus bubalus). | the water buffalo (bubalus bubalus) is important to the economy of several countries, especially in asia and brazil. little is known of the impact of coccidiosis in buffaloes. cattle and buffaloes are considered to have common species of eimeria but critical cross transmissions have not been made because it is difficult to raise these hosts coccidian free. clinical coccidiosis was confirmed post mortem in a 22-day old buffalo calf that died after a 3-4 day illness. oocysts morphologically identi ... | 2008 | 18576888 |
| evaluation of a fluorescence polarization assay for the detection of serum antibodies to brucella abortus in water buffalo (bubalus bubalis). | the fluorescence polarization assay (fpa) was evaluated for the serological diagnosis of brucellosis in water buffalo (bubalus bubalis) in southern italy. this assay uses o-polysaccharide prepared from brucella abortus lipopolysaccharide conjugated with fluorescein isothiocyanate as a tracer. it has many methodological advantages over older, more established tests and can be performed in a fraction of the time. sera from 890 buffalos from the campania region - 526 positive sera and 364 negative ... | 2008 | 18599128 |
| experimental transmission of foot-and-mouth disease among indian buffalo (bubalus bubalis) and from buffalo to cattle. | indian buffalo and cattle were infected experimentally with a serotype o strain of foot-and-mouth disease virus of buffalo origin. whereas intradermolingual inoculation of buffalo produced largely sub-clinical infection, inoculation in the dental pad produced vesicles in the mouth and on the feet. a buffalo infected via the dental pad transmitted infection to cattle and buffalo by direct contact with them for 24h. the contact-exposed buffalo developed (1) delayed-onset clinical signs, and (2) sh ... | 2008 | 18619607 |