Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| ventriculitis due to infection with rhizopus arrhizus. | a 52-year-old heart-lung transplant patient presented to the emergency department with acute onset of neurologic symptoms. mri showed ballooning of the left ventricle, midline shift and contrast enhancement in the anterior horn of the left ventricle. ventricle neuroendoscopy revealed whitish, floccose aerial structures within the left ventricle. brain biopsy cultures grew rhizopus arrhizus. therapy with liposomale amphotericin b and posaconazole was performed. except for hemianopsia and deficits ... | 2015 | 26862476 |
| [construction of an integration vector carrying hygromycin b resistance gene and its genetic transformation in rhizopus oryzae]. | to construct a system of genetic transformation suitable for rhizopus oryzae, we constructed a single-exchange vector pbs-hygro carrying hygromycin b resistance gene (hph) as its selective marker using gene splicing by overlap extension pcr (soe pcr) technique. we introduced this recombinant vector into rhizopus oryzae as 3.819 by peg/cacl2-mediated transformation of protoplast, electroporation of protoplast and germinated spores; and we studied the effects of hydrolysis time, field strength and ... | 2015 | 26762042 |
| a fermentative approach towards optimizing directed biosynthesis of fumaric acid by rhizopus oryzae 1526 utilizing apple industry waste biomass. | the present research account deals with the bioproduction of fumaric acid (fa) from apple pomace ultrafiltration sludge (apus) and apple pomace (ap) through fermentation. the filamentous fungus rhizopus oryzae 1526 was used as a biocatalyst and its morphological impact on fa production was analysed in detail. for submerged fermentation, 40 g l(-1) of total solids concentration of apus, ph 6.0, 30 °c, 200 rpm flask shaking speed and 72 h of incubation were found to be optimum for fa production (2 ... | 2015 | 26615750 |
| purification, cloning and immuno-biochemical characterization of a fungal aspartic protease allergen rhi o 1 from the airborne mold rhizopus oryzae. | fungal allergy is considered as serious health problem worldwide and is increasing at an alarming rate in the industrialized areas. rhizopus oyzae is a ubiquitously present airborne pathogenic mold and an important source of inhalant allergens for the atopic population of india. here, we report the biochemical and immunological features of its 44 kda sero-reactive aspartic protease allergen, which is given the official designation 'rhi o 1'. | 2015 | 26672984 |
| study of metabolic profile of rhizopus oryzae to enhance fumaric acid production under low ph condition. | ensuring a suitable ph is a major problem in industrial organic acid fermentation. to circumvent this problem, we used a metabolic profiling approach to analyze metabolite changes in rhizopus oryzae under different ph conditions. a correlation between fumaric acid production and intracellular metabolic characteristics of r. oryzae was revealed by principal component analysis. the results showed that to help cell survival in the presence of low ph, r. oryzae altered amino acid and fatty acid meta ... | 2015 | 26481229 |
| mediastinitis and bronchial perforations due to mucormycosis. | diabetes mellitus is the most common predisposing condition for mucormycosis, which is emerging as an important invasive fungal infection worldwide. isolated mediastinitis is a very rare presentation of mucormycosis. a 57-year-old woman with uncontrolled type 2 diabetes mellitus and ketoacidosis presented with septic shock and was subsequently found to have mucor mediastinitis with multiple bronchial perforations. the organism was identified as rhizopus oryzae with the help of dna sequencing. | 2015 | 26348693 |
| role of spacer length in interaction between novel gemini imidazolium surfactants and rhizopus oryzae lipase. | an insight into the effects of new ionic liquid-type gemini imidazolium cationic surfactants on the structure and function of the lipases is of prime importance for their potential application. changes in the activity, stability and structure of rhizopus oryzae lipase in the presence of novel gemini surfactants, [c16-3-c16im]br2 and [c16-12-c16im]br2 were probed in the present study. surfactant with shorter spacer length, [c16-3-c16im]br2 was found to be better in improving the hydrolytic activi ... | 2015 | 26318664 |
| metabolic control analysis of l-lactate synthesis pathway in rhizopus oryzae as 3.2686. | the relationship between the metabolic flux and the activities of the pyruvate branching enzymes of rhizopus oryzae as 3.2686 during l-lactate fermentation was investigated using the perturbation method of aeration. the control coefficients for five enzymes, pyruvate dehydrogenase (pdh), pyruvate carboxylase (pc), pyruvate decarboxylase (pdc), lactate dehydrogenase (ldh), and alcohol dehydrogenase (adh), were calculated. our results indicated significant correlations between pdh and pc, pdc and ... | 2015 | 26288952 |
| activation of glycerol metabolic pathway by evolutionary engineering of rhizopus oryzae to strengthen the fumaric acid biosynthesis from crude glycerol. | rhizopus oryzae is strictly inhibited by biodiesel-based by-product crude glycerol, which results in low fumaric acid production. in this study, evolutionary engineering was employed to activate the glycerol utilization pathway for fumaric acid production. an evolved strain g80 was selected, which could tolerate and utilize high concentrations of crude glycerol to produce 14.9g/l fumaric acid with a yield of 0.248g/g glycerol. key enzymes activity analysis revealed that the evolved strain displa ... | 2015 | 26253910 |
| mechanism of adsorptive removal of methylene blue using dried biomass of rhizopus oryzae. | adsorption is an efficient way to remove synthetic dyes from industrial effluent. here, we show mechanism of adsorptive removal of cationic dye methylene blue (mb) from its aqueous solution using dried biomass of rhizopus oryzae as a biosorbent. the optimum ph and temperature for adsorption was found to be 7.0 and 28 °c, respectively. scanning electron microscopy (sem) of the biomass suggested distinct changes in surface topology post-mb adsorption, while fourier transform infrared (ftir) study ... | 2015 | 26234436 |
| [invasive fungal disease (ifd) by filamentous fungi in the valparaíso region, chile, since implementation of rapid laboratory diagnosis]. | invasive fungal diseases (ifd) by filamentous fungi are a common cause of morbidity and mortality in immunocompromised patients, especially those with myeloid leukemia. in 2011 a protocol for the rapid diagnosis of ifd by filamentous fungi was implemented in valparaiso region. | 2015 | 26065456 |
| [effects of pellet characteristics on l-lactic acid fermentation by rhizopus oryzae]. | effects of pellet morphology, diameter, density, and interior structure on l-lactic acid fermentation by rhizopus oryzae were characterized for different inoculum sizes and concentrations of peptone and caco3. | 2015 | 26065280 |
| influence of altered nadh metabolic pathway on the respiratory-deficient mutant of rhizopus oryzae and its l-lactate production. | respiratory-deficient mutants of rhizopus oryzae (r. oryzae) as 3.3461 were acquired by ultraviolet (uv) irradiation to investigate changes in intracellular nadh metabolic pathway and its influence on the fermentation characteristics of the strain. compared with r. oryzae as 3.3461, the intracellular atp level of the respiratory-deficient strain uv-1 decreased by 52.7 % and the glucose utilization rate rose by 8.9 %; when incubated for 36 h, the activities of phosphofructokinase (pfk), hexokinas ... | 2015 | 26047930 |
| lactic acid production from xylose by engineered saccharomyces cerevisiae without pdc or adh deletion. | production of lactic acid from renewable sugars has received growing attention as lactic acid can be used for making renewable and bio-based plastics. however, most prior studies have focused on production of lactic acid from glucose despite that cellulosic hydrolysates contain xylose as well as glucose. microbial strains capable of fermenting both glucose and xylose into lactic acid are needed for sustainable and economic lactic acid production. in this study, we introduced a lactic acid-produc ... | 2015 | 26043971 |
| cell surface display and characterization of rhizopus oryzae lipase in pichia pastoris using sed1p as an anchor protein. | it has been investigated to conduct the surface displaying of lipase from rhizopus oryzae onto the cells of pichia pastoris yeast using sed1p as an anchor protein. a yeast cell surface display plasmid ppiczαa-rol-histag-sed1p was constructed by fusing rol and sed1p gene fragments into the plasmid ppiczαa, followed by introducing recombinant plasmid into p. pastoris cells. surface display levels were monitored by western blot and immunofluorescence microscopy. the activity of displaying lipase ob ... | 2015 | 26013444 |
| repetitive genomic sequences as a substrate for homologous integration in the rhizopus oryzae genome. | the vast number of repetitive genomic elements was identified in the genome of rhizopus oryzae. such genomic repeats can be used as homologous regions for integration of plasmids. here, we evaluated the use of two different repeats: the short (575 bp) rptz, widely distributed (about 34 copies per genome) and the long (2053 bp) rpth, less prevalent (about 15 copies). the plasmid carrying rptz integrated, but did so through a 2256-bp region of homology to the pyrg locus, a unique genomic sequence. ... | 2015 | 25986546 |
| generation of a functionally distinct rhizopus oryzae lipase through protein folding memory. | rhizopus oryzae lipase (rol) has a propeptide at its n-terminus that functions as an intramolecular chaperone and facilitates the folding of mature rol (mrol). in this study, we successfully generated a functionally distinct imprinted mrol (mrolimp) through protein folding memory using a mutated propeptide. the mutated propeptide left its structural memory on mrol and produced mrolimp that exhibited different substrate specificities compared with mrolwt (prepared from the wild type propeptide), ... | 2015 | 25970342 |
| l-lactic acid production by aspergillus brasiliensis overexpressing the heterologous ldha gene from rhizopus oryzae. | lactic acid is the building block of poly-lactic acid (pla), a biopolymer that could be set to replace petroleum-based plastics. to make lactic acid production cost-effective, the production process should be carried out at low ph, in low-nutrient media, and with a low-cost carbon source. yeasts have been engineered to produce high levels of lactic acid at low ph from glucose but not from carbohydrate polymers (e.g. cellulose, hemicellulose, starch). aspergilli are versatile microbial cell facto ... | 2015 | 25935554 |
| engineering scheffersomyces stipitis for fumaric acid production from xylose. | in this work, scheffersomyces stipitis, the yeast with excellent xylose-utilizing ability, was firstly engineered for fumaric acid production from xylose with the heterologous reductive pathway from rhizopus oryzae fm19, and 1.86g/l fumaric acid was produced by the initial strain psrpmf under the oxygen-limited condition. furthermore, three strategies were performed to improve the fumaric acid production, including increasing the reductive pathway activity by codon optimization, blocking the fum ... | 2015 | 25863201 |
| a new strategy for co-composting dairy manure with rice straw: addition of different inocula at three stages of composting. | in considering the impact of inoculation time and the characteristics of composting material and inoculants on the usefulness of inoculation, a new composting strategy has been proposed and studied, in which three inocula were inoculated at three stages of composting process respectively: inoculum a (thermoactinomyces sp. gf1 and gf2) was inoculated before fermentation to increase or maintain high temperature of pile, inoculum b (coprinus cinerea and coprinus comatus) was inoculated after thermo ... | 2015 | 25837785 |
| iron starvation induces apoptosis in rhizopus oryzae in vitro. | mortality associated with mucormycosis remains high despite current antifungals. iron-starvation strategies have been shown to have promising activity against mucorales. we hypothesized that iron starvation enhances apoptosis in rhizopus oryzae. apoptosis was characterized in r. oryzae transformed with rnai plasmid targeting ftr1 expression (iron permease mutant) or empty plasmid grown in iron rich (0.125% fecl3) and iron depleted media (ynb+1mm ferrozine and 1 mm ascorbic acid). increased apopt ... | 2015 | 25830548 |
| cutaneous mucormycosis of scalp and eyelids in a child with type i diabetes mellitus. | scalp mucormycosis in children is extremely rare. we present a case of pediatric scalp mucormycosis caused by rhizopus oryzae in a 9-year-old diabetic girl who was successfully diagnosed and treated with amphotericin b deoxycholate and wound debridement. at 3 months follow up, the patient was stable although she had lost her vision. | 2015 | 25784223 |
| graphene oxide immobilized enzymes show high thermal and solvent stability. | the thermal and solvent tolerance of enzymes is highly important for their industrial use. we show here that the enzyme lipase from rhizopus oryzae exhibits exceptionally high thermal stability and high solvent tolerance and even increased activity in acetone when immobilized onto a graphene oxide (go) nanosupport prepared by staudenmaier and brodie methods. we studied various forms of immobilization of the enzyme: by physical adsorption, covalent attachment, and additional crosslinking. the act ... | 2015 | 25757536 |
| effect of dilution rate and methanol-glycerol mixed feeding on heterologous rhizopus oryzae lipase production with pichia pastoris mut(+) phenotype in continuous culture. | the induction using substrate mixtures is an operational strategy for improving the productivity of heterologous protein production with pichia pastoris. glycerol as a cosubstrate allows for growth at a higher specific growth rate, but also has been reported to be repressor of the expression from the aox1 promoter. thus, further insights about the effects of glycerol are required for designing the induction stage with mixed substrates. the production of rhizopus oryzae lipase (rol) was used as a ... | 2015 | 25740724 |
| enzymatic biodiesel synthesis from yeast oil using immobilized recombinant rhizopus oryzae lipase. | the recombinant rhizopus oryzae lipase (1-3 positional selective), immobilized on relizyme od403, has been applied to the production of biodiesel using single cell oil from candida sp. leb-m3 growing on glycerol from biodiesel process. the composition of microbial oil is quite similar in terms of saponifiable lipids than olive oil, although with a higher amount of saturated fatty acids. the reaction was carried out in a solvent system, and n-hexane showed the best performance in terms of yield a ... | 2015 | 25731926 |
| an unusual ulcer: a case of cutaneous mucormycosis caused by rhizopus oryzae. | mucormycoses are high-mortality infections feared by clinicians worldwide. they predominantly affect immunocompromised hosts and are associated with a spectrum of disease. we describe a case of cutaneous mucormycosis caused by rhizopus oryzae in a patient with multiple risk factors cured with complete surgical excision and a short course of antifungal therapy. | 2015 | 27330940 |
| cloning, expression, and characterization of a novel xylose reductase from rhizopus oryzae. | rhizopus oryzae is valuable as a producer of organic acids via lignocellulose catalysis. r. oryzae metabolizes xylose, which is one component of lignocellulose hydrolysate. in this study, a novel nadph-dependent xylose reductase gene from r. oryzae as 3.819 (roxr) was cloned and expressed in pichia pastoris gs115. homology alignment suggested that the 320-residue protein contained domains and active sites belonging to the aldo/keto reductase family. sds-page demonstrated that the recombinant xyl ... | 2015 | 25709086 |
| enhancement of immobilized lipase activity by design of polymer brushes on a hollow fiber membrane. | a polymer brush possessing aminoethanol (ae) functional groups for lipase immobilization was grafted onto a hollow fiber membrane by radiation-induced graft polymerization. almost the ae groups-grafted polymer brushes unfold through positive charge repulsion between the ae groups, enabling multi-layer immobilization of lipase. the hydroxyl groups in ae can also retain water molecules around hydrophilic part of the lipase. in this study, we controlled the length and density of the polymer brushes ... | 2015 | 25704080 |
| an uncommon cause of allergic fungal sinusitis: rhizopus oryzae. | we report what we believe is the first case of allergic fungal rhinosinusitis (afrs) caused by the fungus rhizopus oryzae. our patient was a 32-year-old woman who presented with unilateral nasal polyps and chronic nasal dysfunction. computed tomography of the sinuses detected left-sided pansinusitis and bone erosion. t2-weighted magnetic resonance imaging demonstrated a signal void that suggested the presence of a fungal infection. the patient underwent unilateral ethmoidectomy. histologic exami ... | 2015 | 25606840 |
| a metabolic-based approach to improve xylose utilization for fumaric acid production from acid pretreated wheat bran by rhizopus oryzae. | in this work, wheat bran (wb) was utilized as feedstock to synthesize fumaric acid by rhizopus oryzae. firstly, the pretreatment process of wb by dilute sulfuric acid hydrolysis undertaken at 100°c for 30min offered the best performance for fumaric acid production. subsequently, through optimizing the seed culture medium, a suitable morphology (0.55mm pellets diameter) of r. oryzae was obtained. furthermore, a metabolic-based approach was developed to profile the differences of intracellular met ... | 2015 | 25594507 |
| a macrokinetic model-based comparative meta-analysis of recombinant protein production by pichia pastoris under aox1 promoter. | an unstructured macrokinetic model for heterologous production of rhizopus oryzae lipase (rol) by a pichia pastoris paox1 based system was developed. mean specific rates and state variables for various fed-batch cultures, under methanol limited and non-limited conditions were used for modeling. the most representative kinetic functions (viz., monod, haldane, pirt and luedeking-piret) were used for the specific rates of cell growth (µ), substrate consumption (qs ) and product formation (qp ). the ... | 2015 | 25546846 |
| protective effect of aqueous extracts from rhizopus oryzae on liver injury induced by carbon tetrachloride in rats. | hepatoprotective effects of rhizopus oryzae/ u-1 aqueous extract (ru) were demonstrated in carbon tetrachloride (ccl4 )-induced liver-injured rats. in order to investigate the ru effects, the rats were administered ru at a dose of 10 or 100 mg/kg of body weight for 10 days before induction of the liver injury by oral administration of ccl4 (125 mg/kg body weight). (i) pretreatment with ru caused a significant decrease in serum lactate dehydrogenase (ldh), alanine aminotransferase (alt) and aspar ... | 2015 | 25496319 |
| rhizopus oryzae lipase immobilized on hierarchical mesoporous silica supports for transesterification of rice bran oil. | the tunable textural properties of self-oriented mesoporous silica were investigated for their suitability as enzyme immobilization matrices to support transesterification of rice bran oil. different morphologies of mesoporous silica (rod-like, rice-like, and spherical) were synthesized and characterized by scanning electron microscopy (sem), transmission electron microscopy (tem), and nitrogen adsorption-desorption isotherms. the surface area, pore size, and ordered arrangement of the pores wer ... | 2015 | 25488500 |
| disseminated mucormycosis due to rhizopus oryzae diagnosed by skin biopsy. | 2015 | 25470108 | |
| efficient co-displaying and artificial ratio control of α-amylase and glucoamylase on the yeast cell surface by using combinations of different anchoring domains. | recombinant yeast strains that display heterologous amylolytic enzymes on their cell surface via the glycosylphosphatidylinositol (gpi)-anchoring system are considered as promising biocatalysts for direct ethanol production from starchy materials. for the effective hydrolysis of these materials, the ratio optimization of multienzyme activity displayed on the cell surface is important. in this study, we have presented a ratio control system of multienzymes displayed on the yeast cell surface by u ... | 2015 | 25432675 |
| effective treatment of cerebral mucormycosis associated with brain surgery. | isolated cerebral mucormycosis is rare. we describe a patient with a brain tumor and a surgically-related rhizopus oryzae brain abscess. her abscess was effectively treated with posaconazole, micafungin and colony-stimulating factor followed by posaconazole alone. to our knowledge, cerebral mucormycosis secondary to brain surgery had not been previously reported. | 2015 | 25420158 |
| outcome of mucormycosis after treatment: report of five cases. | mucormycoses are serious infections caused by filamentous fungi of the order mucorales. they occur most often in immunocompromised patients. we report five cases of mucormycosis in patients hospitalized in the infectious diseases department in sousse - tunisia between 2000 and 2013. they were 4 males and one female, mean age 60 years. three patients were diabetic and one patient had acute leukemia. the locations of mucormycosis were rhinocerebral, rhino-orbital, auricular, pulmonary and cutaneou ... | 2015 | 26137308 |
| high production of fumaric acid from xylose by newly selected strain rhizopus arrhizus rh 7-13-9#. | fumaric acid, as an important material for polymerization, is highly expected to be produced by fermentation of lignocellulosic biomass which is composed of cellulose, hemicellulose and lignin. xylose as the main component of hemicellulose cannot be efficiently utilized by most of the common fermentation. in this study, a new strain rhizopus arrhizus rh 7-13-9# was selected from the r. arrhizus rh 7-13 through a novel convenient and efficient selection method. efficient production of fumaric aci ... | 2015 | 25862014 |
| invasive rhino-orbito-cerebral mucormycosis in a diabetic patient - the need for prompt treatment. | mucormycosis is a rare life threatening fungal infection predominately seen in immunocompromised or diabetic patients. the following case is of a known type ii diabetic patient who presented with sepsis and sudden unilateral loss of vision secondary to infective rhino-orbito-cerebral mucormycosis. treatment of the condition required extensive surgical intervention and medical management for a life saving outcome. | 2015 | 25750854 |
| purification and characterization of a cytochrome c with novel caspase-3 activation activity from the pathogenic fungus rhizopus arrhizus. | members of rhizopus species are the most common cause of mucormycosis, a rare but often fatal fungal infection. host induced pathogen apoptosis and pathogen induced host cell apoptosis are often involved in fungal infections. in many organisms, the release of mitochondrial cytochrome c can trigger apoptosis by activating caspase proteases, but the role of fungal cytochrome c in apoptosis remains unknown. | 2015 | 26334686 |
| two-step biocatalytic process using lipase and whole cell catalysts for biodiesel production from unrefined jatropha oil. | to avoid lipase deactivation by methanol in the enzymatic transesterification process, a two-step biocatalytic process for biodiesel production from unrefined jatropha oil was developed. | 2015 | 26063623 |
| deciphering the uniqueness of mucoromycotina cell walls by combining biochemical and phylogenomic approaches. | most fungi from the mucoromycotina lineage occur in ecosystems as saprobes, although some species are phytopathogens or may induce human mycosis. mucoromycotina represent early diverging models that are most valuable for understanding fungal evolution. here we reveal the uniqueness of the cell wall structure of the mucoromycotina rhizopus oryzae and phycomyces blakesleeanus compared with the better characterized cell wall of the ascomycete neurospora crassa. we have analysed the corresponding po ... | 2015 | 25143134 |
| evaluation of the matrix-assisted laser desorption/ionization time-of-flight mass spectrometry bruker biotyper for identification of penicillium marneffei, paecilomyces species, fusarium solani, rhizopus species, and pseudallescheria boydii. | we evaluated the performance of matrix-assisted laser desorption ionization-time of flight mass spectrometry (maldi-tof ms), the maldi bruker biotyper system (microflex lt; bruker daltonik gmbh, bremen, germany), on the identification of 50 isolates of clinically encountered molds, including penicillium marneffei (n = 28), paecilomyces species (n = 12), fusarium solani (n = 6), rhizopus species (n = 3), and pseudallescheria boydii (n = 1). the isolates were identified to species levels by sequen ... | 2015 | 26217315 |
| screening of accurate clones for gene synthesis in yeast. | methods for error-less gene synthesis are desired because synthesized genes often contain mutations. by cloning pcr-assembled oligonucleotide fragments fused to a selection marker in yeast, we developed a novel method to screen accurate clones in gene synthesis. as a model case, the 555-bp luciferase gene from gaussia princeps (gluc) was synthesized to contain yeast-optimized codons (called ygluc hereafter). after standard pcr-mediated oligonucleotide assembly, many clones showed no luciferase a ... | 2015 | 25201012 |
| in vitro activity of isavuconazole and comparators against clinical isolates of the mucorales order. | the in vitro activity of isavuconazole against mucorales isolates measured by eucast e.def 9.2 and clsi m38-a2 methodologies was investigated in comparison with those of amphotericin b, posaconazole, and voriconazole. seventy-two isolates were included: 12 of lichtheimia corymbifera, 5 of lichtheimia ramosa, 5 of group i and 9 of group ii of mucor circinelloides, 9 of rhizomucor pusillus, 26 of rhizopus microsporus, and 6 of rhizopus oryzae. species identification was confirmed by internal trans ... | 2015 | 26438494 |
| evaluation of antimicrobial activity of extracts of in vivo and in vitro grown vinca rosea l. (catharanthus roseus) against pathogens. | the antimicrobial activity of vinca rosea was evaluated against pathogenic bacterial strains (bacillus subtilis, b. licheniformis and azotobacter sp.) and fungal strains (asprgillus niger, alternaria solani and rhizopus oryzae) using agar well diffusion method. methanolic extracts of in vivo leaf, in vitro leaf, in vitro calluses of leaf, nodal and fruit explants were used and exhibited antimicrobial activity as indicated by minimum inhibitory concentration (mic). in vitro extracts showed better ... | 2015 | 26004716 |
| multicenter evaluation of mic distributions for epidemiologic cutoff value definition to detect amphotericin b, posaconazole, and itraconazole resistance among the most clinically relevant species of mucorales. | clinical breakpoints (cbps) have not been established for the mucorales and any antifungal agent. in lieu of cbps, epidemiologic cutoff values (ecvs) are proposed for amphotericin b, posaconazole, and itraconazole and four mucorales species. wild-type (wt) mic distributions (organisms in a species-drug combination with no detectable acquired resistance mechanisms) were defined with available pooled clsi mics from 14 laboratories (argentina, australia, canada, europe, india, mexico, and the unite ... | 2015 | 25583714 |
| biosynthesis of human diazepam and clonazepam metabolites. | a screening of fungal and microbial strains allowed to select the best microorganisms to produce in high yields some of the human metabolites of two benzodiazepine drugs, diazepam and clonazepam, in order to study new pharmacological activities and for chemical standard proposes. among the microorganisms tested, cunninghamella echinulata atcc 9244 and rhizopus arrhizus atcc 11145 strains, were the most active producers of the mains metabolites of diazepam which included demethylated, hydroxylate ... | 2015 | 25655722 |
| modification of prenylated stilbenoids in peanut (arachis hypogaea) seedlings by the same fungi that elicited them: the fungus strikes back. | aspergillus oryzae and rhizopus oryzae were compared for inducing the production of prenylated stilbenoids in peanut seedlings. the fungus was applied at two different time points: directly after soaking (day 1) or after 2 days of germination (day 3). aspergillus- and rhizopus-elicited peanut seedlings accumulated an array of prenylated stilbenoids, with overlap in compounds induced, but also with compounds specific to the fungal treatment. the differences were confirmed to be due to modificatio ... | 2015 | 26458982 |
| a prospective study of mucormycosis in north india: experience from a tertiary care hospital. | mucormycosis is an emerging angioinvasive infection caused by the ubiquitous filamentous fungi of the order mucorales and class of mucormycetes. we conducted a prospective study of 38 patients who were diagnosed as having mucormycosis in a tertiary care hospital during january 2010 to june 2011. the cases were analyzed regarding the site of involvement, underlying disease and species of fungi isolated, antifungal susceptibility pattern of the isolates, and outcome of therapy. the mean age of the ... | 2015 | 25587084 |
| functional properties of filamentous fungi isolated from the indonesian fermented dried cassava, with particular application on poultry. | the study aimed to evaluate the probiotic properties, antioxidant activity and fermentative capacity of acremonium charticola and rhizopus oryzae isolated from the indonesian fermented dried cassava, with particular application on poultry. a. charticola inhibited the growth of escherichia coli and aspergillus flavus. a. charticola and r. oryzae grew in potato dextrose agar (pda) adjusted to ph 3 and 8 or in pda supplemented with bile salt up to 0.8%. after soaking for 8 hr, the survival rate of ... | 2015 | 26839501 |
| identification of a critical determinant that enables efficient fatty acid synthesis in oleaginous fungi. | microorganisms are valuable resources for lipid production. what makes one microbe but not the other able to efficiently synthesize and accumulate lipids is poorly understood. in the present study, global gene expression prior to and after the onset of lipogenesis was determined by transcriptomics using the oleaginous fungus mortierella alpina as a model system. a core of 23 lipogenesis associated genes was identified and their expression patterns shared a high similarity among oleaginous microb ... | 2015 | 26059272 |
| coccidioides endospores and spherules draw strong chemotactic, adhesive, and phagocytic responses by individual human neutrophils. | coccidioides spp. are dimorphic pathogenic fungi whose parasitic forms cause coccidioidomycosis (valley fever) in mammalian hosts. we use an innovative interdisciplinary approach to analyze one-on-one encounters between human neutrophils and two forms of coccidioides posadasii. to examine the mechanisms by which the innate immune system coordinates different stages of the host response to fungal pathogens, we dissect the immune-cell response into chemotaxis, adhesion, and phagocytosis. our singl ... | 2015 | 26070210 |
| a facile synthesis of novel miconazole analogues and the evaluation of their antifungal activity. | four novel miconazole analogues (8-11) were synthetized and evaluated for activity against four filamentous fungi (mucor hiemalis, aspergillus fumigatus, trichosporon cutaneum, and rhizopus oryzae) and eight species of candida as yeast specimens. compounds 9 and 10 showed very good activity when evaluated in yeast (mic 0.112 and 0.163 μg/ml) compared to the reference compound, itraconazole (mic 0.067 μg/ml). the best antifungal activity in filamentous strains was shown by compound 9. hence compo ... | 2015 | 25989345 |
| assessment of microbial contamination within working environments of different types of composting plants. | the objective of the study was to determine the degree of microbiological contamination, type of microflora, bioaerosol particle size distribution, and concentration of endotoxins in dust in different types of composting plants. in addition, this study provides a list of indicator microorganisms that pose a biological threat in composting facilities, based on their prevalence within the workplace, source of isolation, and health hazards. we undertook microbiological analysis of the air, work sur ... | 2015 | 25947216 |
| glucocorticosteroids do not impact directly growth rate and biomass of rhizopus arrhizus (syn. r. oryzae) in vitro. | glucocorticoid (gc) use is a common risk factor for invasive fungal infections. this is attributed to the complex dysregulation of immunity caused by gcs. however, studies have demonstrated increased growth with gc exposure for some molds, such as aspergillus fumigatus and exserohilum rostratum. no such data exist for mucorales. therefore, we investigated the influence of gc exposure on the growth of rhizopus arrhizus (syn. r. oryzae) in different culture media and in different atmospheres. we m ... | 2015 | 25942104 |
| evaluation of aerial microbial pollutants in al-haram al-nabawi during pilgrimage of 2013. | al-madinah al-munawwarah is the second holiest site in islam. the possibility of new emerging microbes is valid due to the increased number of pilgrims. the objectives of the current study were to estimate the numbers of fungi and bacteria inside and outside al-haram al-nabawi and to find whether new bacterial and fungal species have emerged compared to previous studies. air samples were collected twice a day from 12 spots and four directions during the pilgrim year of 2013 for four consecutive ... | 2015 | 28053593 |
| mycofabricated biosilver nanoparticles interrupt pseudomonas aeruginosa quorum sensing systems. | quorum sensing (qs) is a chemical communication process that pseudomonas aeruginosa uses to regulate virulence and biofilm formation. disabling of qs is an emerging approach for combating its pathogenicity. silver nanoparticles (agnps) have been widely applied as antimicrobial agents against human pathogenic bacteria and fungi, but not for the attenuation of bacterial qs. here we mycofabricated agnps (mfagnps) using metabolites of soil fungus rhizopus arrhizus brs-07 and tested their effect on q ... | 2015 | 26347993 |
| fob1 and fob2 proteins are virulence determinants of rhizopus oryzae via facilitating iron uptake from ferrioxamine. | dialysis patients with chronic renal failure receiving deferoxamine for treating iron overload are uniquely predisposed for mucormycosis, which is most often caused by rhizopus oryzae. although the deferoxamine siderophore is not secreted by mucorales, previous studies established that rhizopus species utilize iron from ferrioxamine (iron-rich form of deferoxamine). here we determined that the cbs domain proteins of fob1 and fob2 act as receptors on the cell surface of r. oryzae during iron upta ... | 2015 | 25974051 |
| new insight into microbial diversity and functions in traditional vietnamese alcoholic fermentation. | the roles of microorganisms in traditional alcoholic fermentation are often assumed based on abundance in the starter and activity in pure culture. there is a serious lack of hard evidence on the behavior and activity of individual microbial species during the actual fermentation process. in this study, microbial succession and metabolite changes during 7days of traditional vietnamese alcoholic fermentation were monitored. special attention was devoted to starch degradation. in total, 22 microbi ... | 2016 | 27236224 |
| antifungal activity of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides: a novel type of compound afforded by azide-enolate (3+2) cycloaddition. | the first report of 1'-homo-n-1,2,3-triazol-bicyclic carbonucleosides (7a and 7b) is described herein. azide-enolate (3+2) cycloaddition afforded the synthesis of this novel type of compound. antifungal activity was evaluated in vitro against four filamentous fungi (aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae and mucor hiemalis) as well as nine species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 7a and 7b are promising candidates for co ... | 2016 | 27656774 |
| enose technology can detect and classify human pathogenic molds in vitro: a proof-of-concept study of aspergillus fumigatus and rhizopus oryzae. | invasive pulmonary mold disease (ipmd) is often fatal in neutropenic patients. this is because ipmd is difficult to diagnose timely, especially when non-aspergillus molds are the causative agent, as they are usually not associated with a positive galactomannan assay. in 2013 we showed that exhaled breath analysis might be used to diagnose invasive aspergillosis through profiling of patterns in exhaled volatile organic compounds (vocs) by electronic nose (enose) technology. the current study aime ... | 2016 | 27447026 |
| acute invasive fungal rhinosinusitis: our experience with 18 cases. | acute invasive fungal rhinosinusitis (aifrs) is a rapidly progressive life threatening infection that is seen most commonly among immunocompromised patients. we present a case series of 18 patients clinically and histopathologically diagnosed with aifrs with a mean follow-up of 9.11 ± 2.51 months (range 6-17). demographic data, apparent symptoms and signs, underlying disorders, and outcomes are discussed. the mean age was 39.56 ± 20.66 years (range 2-75). the most common underlying diseases were ... | 2016 | 27272179 |
| azide-enolate 1,3-dipolar cycloaddition in the synthesis of novel triazole-based miconazole analogues as promising antifungal agents. | seven miconazole analogs involving 1,4,5-tri and 1,5-disubstituted triazole moieties were synthesized by azide-enolate 1,3-dipolar cycloaddition. the antifungal activity of these compounds was evaluated in vitro against four filamentous fungi, including aspergillus fumigatus, trichosporon cutaneum, rhizopus oryzae, and mucor hiemalis as well as three species of candida spp. as yeast specimens. these pre-clinical studies suggest that compounds 4b, 4d and 7b can be considered as drug candidates fo ... | 2016 | 26890112 |
| identification and characterization of haemofungin, a novel antifungal compound that inhibits the final step of haem biosynthesis. | during recent decades, the number of invasive fungal infections among immunosuppressed patients has increased significantly, whereas the number of effective systemic antifungal drugs remains low and unsatisfactory. the aim of this study was to characterize a novel antifungal compound, cw-8/haemofungin, which we previously identified in a screen for compounds affecting fungal cell wall integrity. | 2016 | 26747101 |
| stimulation with lysates of aspergillus terreus, candida krusei and rhizopus oryzae maximizes cross-reactivity of anti-fungal t cells. | invasive fungal diseases caused by filamentous fungi and yeasts are significant causes of morbidity and mortality in immunosuppressed hematology patients. we previously published a method to expand aspergillus fumigatus-specific t cells for clinical cell therapy. in the present study, we investigated expansion of t cells specific for other fungal pathogens and creation of a broadly reactive panfungal t-cell product. | 2016 | 26552765 |
| discrimination of aspergillosis, mucormycosis, fusariosis, and scedosporiosis in formalin-fixed paraffin-embedded tissue specimens by use of multiple real-time quantitative pcr assays. | in a retrospective multicenter study, 102 formalin-fixed paraffin-embedded (ffpe) tissue specimens with histopathology results were tested. two 4- to 5-μm ffpe tissue sections from each specimen were digested with proteinase k, followed by automated nucleic acid extraction. multiple real-time quantitative pcr (qpcr) assays targeting the internal transcribed spacer 2 (its2) region of ribosomal dna, using fluorescently labeled primers, was performed to identify clinically important genera and spec ... | 2016 | 27605714 |
| stress response in medically important mucorales. | mucorales are saprobes, ubiquitously distributed and able to infect a heterogeneous population of human hosts. the fungi require robust stress responses to survive in human host. we tested the growth of mucorales in the presence of different abiotic stress. eight pathogenic species of mucorales, including rhizopus arrhizus, rhizopus microsporus, rhizomucor pusillus, apophysomyces elegans, licthemia corymbifera, cunninghamella bertholletiae, syncephalastrum racemosum and mucor racemosus, were exp ... | 2016 | 27292160 |
| synthesis, characterization and biological approach of metal chelates of some first row transition metal ions with halogenated bidentate coumarin schiff bases containing n and o donor atoms. | the impregnation of halogen atoms in a molecule is an emerging trend in pharmaceutical chemistry. the presence of halogens (cl, br, i and f) increases the lipophilic nature of molecule and improves the penetration of lipid membrane. the presence of electronegative halogen atoms increases the bio- activity of core moiety. in the present study, co(ii), ni(ii) and cu(ii) complexes are synthesised using schiff bases (hl(i) and hl(ii)), derived from 8-formyl-7-hydroxy-4-methylcoumarin/3-chloro-8-form ... | 2016 | 26874303 |
| pleosporalone a, the first azaphilone characterized with aromatic a-ring from a marine-derived pleosporales sp. fungus. | a fungal strain, pleosporales sp. cf09-1, was isolated from marine sediment collected from the bohai sea. a novel azaphilone derivative, named pleosporalone a (1), along with two known analogues, cohaerins a and b (2 and 3), were obtained and identified from the culture extract of pleosporales sp. cf09-1. their planar structures were elucidated by detailed analysis of spectroscopic data and by comparison with related known compounds. pleosporalone a (1) represents the first azaphilone derivative ... | 2016 | 27385435 |
| fumarate production by torulopsis glabrata: engineering heterologous fumarase expression and improving acid tolerance. | fumarate is a well-known biomass building block compound. however, the poor catalytic efficiency of fumarase is one of the major factors preventing its widespread production. to address this issue, we selected residues 159hpnd162 of fumarase from rhizopus oryzae as targets for site-directed mutagenesis based on molecular docking analysis. twelve mutants were generated and characterized in detail. kinetic studies showed that the km values of the p160a, p160t, p160h, n161e, and d162w mutants were ... | 2016 | 27711153 |
| immunoreactivity, sensory and physicochemical properties of fermented soy protein isolate. | the effect of induced liquid state fermentation (bacillus subtilis, rhizopus oryzae, saccharomyces cerevisiae, lactobacillus helveticus) on the immunoreactivity, physicochemical and sensory properties of soy protein isolate (spi) was studied. l. helveticus revealed the most abundant reduction in terms of immunoreactivity within soluble protein fractions, up to 100%, which could be measured by in vitro sandwich elisa using mouse monoclonal anti-glym5 antibodies (mabs). almost no binding was found ... | 2016 | 27006235 |
| antioxidant and neuroprotective effects of doenjang prepared with rhizopus, pichia, and bacillus. | a new type of doenjang was manufactured by mixing soaked soybean, koji (rhizopus oryzae), cheonggukjang (bacillus amyloliquefaciens mj1-4 and b. amyloliquefaciens emd17), and pichia farinosa sy80 as a yeast, salt, and water, followed by fermentation with koji that was made by fermenting whole wheat with r. oryzae. the mixed culture doenjang was designed to have a more palatable flavor and stronger biological activities than the conventional product. the extract of mixed culture doenjang showed h ... | 2016 | 27752498 |
| reclamation of dpk hydrocarbon polluted agricultural soil using a selected bulking agent. | in the present study, laboratory scale bioremediation of dual purpose kerosene (dpk) hydrocarbon polluted soil using bulking agent (saw dust) was carried out. the effect of different parameters such as total petroleum hydrocarbon (tph), dehydrogenase activity (dhase) and ph on bioremediation performance were evaluated. studied parameters such as microbial dynamics, percentage degradation (95.20%), dhase (8.20 ± 0.43) were found to be higher in saw dust amended system and significantly differed w ... | 2016 | 26934642 |
| decreased pseudomonas aeruginosa biofilm formation on nanomodified endotracheal tubes: a dynamic lung model. | ventilator-associated pneumonia (vap) is a serious complication of mechanical ventilation that has been shown to be associated with increased mortality rates and medical costs in the pediatric intensive care unit. currently, there is no cost-effective solution to the problems posed by vap. endotracheal tubes (etts) that are resistant to bacterial colonization and that inhibit biofilm formation could provide a novel solution to the problems posed by vap. the objective of this in vitro study was t ... | 2016 | 27563242 |
| co-production of fungal biomass derived constituents and ethanol from citrus wastes free sugars without auxiliary nutrients in airlift bioreactor. | the potential of two zygomycetes fungi, mucor indicus and rhizopus oryzae, in assimilating citrus waste free sugars (cwfs) and producing fungal chitosan, oil, and protein as well as ethanol was investigated. extraction of free sugars from citrus waste can reduce its environmental impact by decreasing the possibility of wild microorganisms growth and formation of bad odors, a typical problem facing the citrus industries. a total sugar concentration of 25.1 g/l was obtained by water extraction of ... | 2016 | 26927089 |
| production and optimization of biodiesel using mixed immobilized biocatalysts in packed bed reactor. | vegetable oils are used as raw materials for biodiesel production using transesterification reaction. several methods for the production of biodiesel were developed using chemical (alkali and acidic compounds) and biological catalysts (lipases). biodiesel production catalyzed by lipases is energy and cost-saving processes and is carried out at normal temperature and pressure. the need for an efficient method for screening larger number of variables has led to the adoption of statistical experime ... | 2016 | 25940482 |
| heterologous expression of an active chitin synthase from rhizopus oryzae. | chitin synthases are highly important enzymes in nature, where they synthesize structural components in species belonging to different eukaryotic kingdoms, including kingdom fungi. unfortunately, their structure and the molecular mechanism of synthesis of their microfibrilar product remain largely unknown, probably because no fungal active chitin synthases have been isolated, possibly due to their extreme hydrophobicity. in this study we have turned to the heterologous expression of the transcri ... | 2016 | 27777036 |
| antibacterial effects of biosynthesized silver nanoparticles on surface ultrastructure and nanomechanical properties of gram-negative bacteria viz. escherichia coli and pseudomonas aeruginosa. | understanding the interactions of silver nanoparticles (agnps) with the cell surface is crucial for the evaluation of bactericidal activity and for advanced biomedical and environmental applications. biosynthesis of agnps was carried out through in situ reduction of silver nitrate (agno3) by cell free protein of rhizopus oryzae and the synthesized agnps was characterized by uv-vis spectroscopy, high resolution transmission electron microscopy (hrtem), dynamic light scattering (dls), ζ-potential ... | 2016 | 26829373 |
| food waste fermentation to fumaric acid by rhizopus arrhizus rh7-13. | fumaric acid as a four-carbon unsaturated dicarboxylic acid is widely used in the food and chemical industries. food waste (fw), rich in carbohydrates and protein, is a promising potential feedstock for renewable bio-based chemicals. in this research, we investigated the capability of rhizopus arrhizus rh7-13 in producing fumaric acid from fw. the liquid fraction of the fw (l-fw) was proven to be the best seed culture medium in our research. when it was however used to be fermentation medium, th ... | 2016 | 27387957 |
| erratum to: purification and characterization of a cytochrome c with novel caspase-3 activation activity from the pathogenic fungus rhizopus arrhizus. | 2016 | 26895854 | |
| a case of rhino-orbital mucormycosis in diabetes with haematogenous cerebral spread. | we document the first case of haematogenous cerebral spread in rhizopus arrhizus rhino-orbital mucormycosis, and of posaconazole related adrenal insufficiency. a patient presenting with diabetic ketoacidosis and sinusitis was treated with right medial maxillectomy, ethmoidectomy and iv liposomal amphotericin. orbital exenteration was performed after intraorbital spread of infection. iv caspofungin and posaconazole was added but complicated by adrenal insufficiency. mri revealed a new left lentif ... | 2016 | 27766196 |
| asymmetric bioconversion of acetophenone in nano-sized emulsion using rhizopus oryzae. | the fungal morphologies and pellet sizes were controlled in acetophenone reduction by rhizopus oryzae. the acetophenone conversion and (s)-phenylethanol enantiomeric excesses (e.e.) reached the peak after 72 h of incubation when using pellets with 0.54 mm diameter, which showed an excellent performance compared with suspended mycelia, clumps, and pellets with 0.65 or 0.75 mm diameter. furthermore, nano-sized acetophenone was used as a substrate to improve the performances of biotransformation wo ... | 2016 | 26403817 |
| enhancement and modeling of microparticle-added rhizopus oryzae lactic acid production. | lactic acid has a wide industrial application area and can be produced by fungal strains. however, excessive bulk growth form of fungi during the fermentations is a major problem, which limits the fermentation performance. microparticles are excellent tools to prevent bulk fungal growth and provide homogenized fermentation broth to increase uniformity and the prediction performance of the models. therefore, in this study, addition of aluminum oxide and talcum microparticles into fermentations wa ... | 2016 | 26658984 |
| assessing adsorption of polycyclic aromatic hydrocarbons on rhizopus oryzae cell wall components with water-methanol cosolvent model. | the contribution of different fungal cell wall components in adsorption of polycyclic aromatic hydrocarbons (pahs) is still unclear. we isolated rhizopus oryzae cell walls components with sequential extraction, characterized functional groups with nexafs spectra, and determined partition coefficients of pahs on cell walls and cell wall components with cosolvent model. spectra of nexafs indicated that isolated cell walls components were featured with peaks at ~532.7 and ~534.5ev energy. the lipid ... | 2016 | 26655233 |
| enzymatic hydrolysis of polyester thin films: real-time analysis of film mass changes and dissipation dynamics. | cleavage of ester bonds by extracellular microbial hydrolases is considered a key step during the breakdown of biodegradable polyester materials in natural and engineered systems. here we present a novel analytical approach for simultaneous detection of changes in the masses and rigidities of polyester thin films during enzymatic hydrolysis using a quartz crystal microbalance with dissipation monitoring (qcm-d). in experiments with poly(butylene succinate) (pbs) and the lipase of rhizopus oryzae ... | 2016 | 26599203 |
| variation in accumulation of isoflavonoids in phaseoleae seedlings elicited by rhizopus. | seeds from seven species of tribe phaseoleae, i.e. phaseolus, vigna, lablab and psophocarpus, were investigated for inducibility of isoflavonoids by germination with or without subsequent elicitation with rhizopus oryzae. germination alone poorly induced isoflavonoid production (in the range of 0.2-0.7 mg representative compound equivalents (rce)/g dw), whereas application of rhizopus onto the seedlings increased the isoflavonoid content considerably (in the range of 0.5-3.3 mg rce/g dw). the in ... | 2016 | 26593543 |
| cloning and expression of trehalose-6-phosphate synthase 1 from rhizopus oryzae. | trehalose is a reducing disaccharide acting as a protectant against environmental stresses in many organisms. in fungi, trehalose-6-phosphate synthase 1 (tps1) plays a key role in the biosynthesis of trehalose. in this study, a full-length cdna from rhizopus oryzae encoding tps1 (designated as rotps1) was isolated. the rotps1 cdna is composed of 2505 nucleotides and encodes a protein of 834 amino acids with a molecular mass of 97.8 kda. the amino acid sequence of rotps1 has a relatively high hom ... | 2016 | 26567772 |
| compositional changes in (iso)flavonoids and estrogenic activity of three edible lupinus species by germination and rhizopus-elicitation. | the effects of germination and elicitation on (iso)flavonoid composition of extracts from three edible lupine species (lupinus luteus, lupinus albus, lupinus angustifolius) were determined by rp-uhplc-ms(n). the total (iso)flavonoid content of lupine increased over 10-fold upon germination, with the total content and composition of isoflavonoids more affected than those of flavonoids. glycosylated isoflavones were the most predominant compounds found in lupine seedlings. lesser amounts of isofla ... | 2016 | 26749476 |
| biodiesel production from nannochloropsis gaditana lipids through transesterification catalyzed by rhizopus oryzae lipase. | biodiesel (fatty acid methyl esters, fames) was produced from saponifiable lipids (sls) extracted from wet nannochloropsis gaditana biomass using methanolysis catalyzed by rhizopus oryzae intracellular lipase. sls were firstly extracted with ethanol to obtain 31 wt% pure sls. but this low sl purity also gave a low biodiesel conversion (58%). this conversion increased up to 80% using sls purified by crystallization in acetone (95 wt% purity). polar lipids play an important role in decreasing the ... | 2016 | 26735878 |
| impacts of lignocellulose-derived inhibitors on l-lactic acid fermentation by rhizopus oryzae. | inhibitors generated in the pretreatment and hydrolysis of corn stover and corn cob were identified. in general, they inhibited cell growth, lactate dehydrogenase, and lactic acid production but with less or no adverse effect on alcohol dehydrogenase and ethanol production in batch fermentation by rhizopus oryzae. furfural and 5-hydroxymethyl furfural (hmf) were highly toxic at 0.5-1 g l(-1), while formic and acetic acids at less than 4 g l(-1) and levulinic acid at 10 g l(-1) were not toxic. am ... | 2016 | 26724548 |
| droplet digital pcr-aided screening and characterization of pichia pastoris multiple gene copy strains. | pichia (syn. komagataella) pastoris is a widely used yeast platform for heterologous protein production. expression cassettes are usually stably integrated into the genome of this host via homologous recombination. although increasing gene dosage is a powerful strategy to improve recombinant protein production, an excess in the number of gene copies often leads to decreased product yields and increased metabolic burden, particularly for secreted proteins. we have constructed a series of strains ... | 2016 | 26704939 |
| in vitro antifungal susceptibility of clinical species belonging to aspergillus genus and rhizopus oryzae. | among filamentous fungal pathogens, aspergillus spp. and zygomycetes account for highest rates of morbidity and mortality among immunocompromised patients. recently developed antifungal drugs offer the potential to improve management and therapeutic outcomes of fungal infections. the aim of this study was to analyse the in vitro activities of voriconazole, itraconazole, amphotericin b and caspofungin against clinical isolates of aspergillus spp. and rhizopus oryzae. | 2016 | 26852191 |
| assay of antioxidant potential of two filamentous fungi isolated from the indonesian fermented dried cassava. | the antioxidant capacity and antioxidant constituents of two filamentous fungi (acremonium charticola and rhizopus oryzae) isolated from the indonesian fermented dried cassava (gathot) were evaluated in the present study. the antioxidant capacity of the fungal crude extracts was assessed based on the 2,2'-azino-bis(3-ethyl-benzthiazolin-6-sulfonicacid) (abts) method. total phenolics were determined based on the folin-ciocalteu method, while the flavonoids content in the fungal extracts was deter ... | 2016 | 26848695 |
| biocatalytic synthesis of novel partial esters of a bioactive dihydroxy 4-methylcoumarin by rhizopus oryzae lipase (rol). | highly regioselective acylation has been observed in 7,8-dihydroxy-4-methylcoumarin (dhmc) by the lipase from rhizopus oryzae suspended in tetrahydrofuran (thf) at 45 °c using six different acid anhydrides as acylating agents. the acylation occurred regioselectively at one of the two hydroxy groups of the coumarin moiety resulting in the formation of 8-acyloxy-7-hydroxy-4-methylcoumarins, which are important bioactive molecules for studying biotansformations in animals, and are otherwise very di ... | 2016 | 27834873 |
| fungal surface protein mediated one-pot synthesis of stable and hemocompatible gold nanoparticles. | despite their large secretome and wide applications in bioprocesses, fungi remain underexplored in metal nanoparticles (mnp) biosynthesis. previous studies have shown that cell surface proteins of rhizopus oryzae play a crucial role in biomineralization of au(iii) to produce gold nanoparticles (aunps). therefore, it is hypothesized that purified cell surface protein may produce in vitro aunps with narrow size distribution for biomedical and biocatalytic applications. however, different protein e ... | 2016 | 27866629 |
| omics-based approaches reveal phospholipids remodeling of rhizopus oryzae responding to furfural stress for fumaric acid-production from xylose. | in order to relieve the toxicity of furfural on rhizopus oryzae fermentation, the molecular mechanism of r. oryzae responding to furfural stress for fumaric acid-production was investigated by omics-based approaches. in metabolomics analysis, 29 metabolites including amino acid, sugars, polyols and fatty acids showed significant changes for maintaining the basic cell metabolism at the cost of lowering fumaric acid production. to further uncover the survival mechanism, lipidomics was carried out, ... | 2016 | 27697734 |
| utilization of discard bovine bone as a support for immobilization of recombinant rhizopus oryzae lipase expressed in pichia pastoris. | in this study the possibility of using discard bovine bone as support for immobilization of rhizopus oryzae lipase expressed in pichia pastoris was analyzed. discard bovine bone were milled and then subjected to a chemical treatment with acetone in order to remove lipids and blood traces. two types of supports were evaluated: bovine bone and calcined bovine bone for 2 h at 600°c. supports were characterized by: icp, sem, xrd, ftir, xps, and n2 adsorption isotherms. calcined bovine bone presented ... | 2016 | 27339042 |
| comparison of lipases for in vitro models of gastric digestion: lipolysis using two infant formulas as model substrates. | the aim of this study was to find a lipase suitable as a surrogate for human gastric lipase (hgl), since the development of predictive gastrointestinal lipolysis models are hampered by the lack of a lipase with similar digestive properties as hgl. three potential surrogates for hgl; rhizopus oryzae lipase (rol), rabbit gastric lipase (rgl) and recombinant hgl (rhgl), were used to catalyze the in vitro digestion of two infant formulas (a medium-chain triacylglyceride enriched formula (mc-if) and ... | 2016 | 27711870 |
| the environmental source of emerging apophysomyces variabilis infection in india. | the rare mucoraceous fungus, apophysomyces species complex ranks second after rhizopus arrhizus causing mucormycosis in india. the source of this agent in the environment is not clearly known. we conducted an environmental study to find its presence in indian soil. the soil samples from different geographical locations were analyzed for isolation of mucorales. rhizopus arrhizus (24.6%) was most commonly isolated from soil, followed by lichtheimia spp. (23.2%), cunninghamella spp. (21.7%), rhizop ... | 2016 | 27118802 |