Publications

selective switch between latency and lytic replication of kaposi's sarcoma herpesvirus and epstein-barr virus in dually infected body cavity lymphoma cells.	the bc-1 cell line, derived from a body cavity-based, b-cell lymphoma, is dually infected with epstein-barr virus (ebv) and kaposi's sarcoma-associated herpesvirus (kshv). in these studies, the relationships between these two gammaherpesviruses and bc-1 cells were characterized and compared. single-cell cloning experiments suggested that all bc-1 cells contain both genomes. in more than 98% of cells, both viruses were latent. the two viruses could be differentially induced into their lytic cycle ...	1997	8985352
kaposi's sarcoma-associated herpesvirus gene expression in endothelial (spindle) tumor cells.	the recent discovery of dna sequences of a new human herpesvirus in kaposi's sarcoma (ks) has fueled speculation that this virus might cause ks. the mere presence, however, of a virus in a complex multicellular tumor like ks could just as well be construed as evidence of a passenger agent. we sought stronger evidence linking the ks-associated herpesvirus (kshv) to tumor formation by using in situ hybridization to investigate the specificity, constancy, and timing of kshv gene expression in ks tu ...	1997	8985403
human herpesvirus 8 encodes a homolog of interleukin-6.	kaposi's sarcoma is a multifocal lesion that is reported to be greatly influenced by cytokines such as interleukin-6 (il-6) and oncostatin m. dna sequences of a novel human gammaherpesvirus, termed human herpesvirus 8 (hhv-8) or kaposi sarcoma-associated herpesvirus, have been identified in all epidemiological forms of kaposi's sarcoma with high frequency. the presence of hhv-8 dna is also clearly associated with certain b-cell lymphomas (body cavity-based lymphomas) and multicentric castleman's ...	1997	8985427
causing cancer by remote control?		1997	9206838
detection of human herpesvirus 8 dna in kaposi's sarcoma lesions and peripheral blood of human immunodeficiency virus-positive patients and correlation with serologic measurements.	polymerase chain reaction (pcr) was used to examine human herpesvirus 8 (hhv-8) dna from kaposi's sarcoma (ks) lesions, normal skin, and peripheral blood mononuclear cells (pbmc) from human immunodeficiency virus (hiv)-infected patients who did or did not have ks. of 9 ks biopsies, 8 were positive for five hhv-8 open-reading frames and ranged from 1 viral genome per 2.5-12.7 cells. two putative replicative gene rnas were detected by reverse transcription-pcr at low levels in 1 ks lesion. hhv-8 d ...	1997	9207353
frequent detection of kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) dna in saliva of human immunodeficiency virus-infected men: clinical and immunologic correlates.	the prevalence, quantity, temporal pattern, and clinical and immunologic correlates of shedding of kaposi's sarcoma (ks)-associated herpesvirus (kshv; or human herpesvirus [hhv]-8) dna in saliva were studied. kshv dna was detected in saliva from 18 (75%) of 24 human immunodeficiency virus (hiv)-positive patients with ks and from 1 of 1 hiv-negative patient with ks, 3 (15%) of 20 hiv-positive patients without ks, and none of 24 controls. kshv dna levels ranged from 10(2.4) to 10(6) copies/ml and ...	1997	9207354
hhv-8-positive body-cavity-based lymphoma: a novel lymphoma entity.		1997	9207392
herpes virus (kshv) associated kaposi sarcoma in a 3-year-old child with non-hiv-induced immunodeficiency.	a 3-year-old boy of german descent suffered from two episodes of streptococcus pneumoniae meningitis within 2 months. one month previously, the first skin lesion of kaposi sarcoma (ks) had been observed behind his right ear. during the following 2 years ks disseminated not only mucocutaneously but also to visceral organs. immunological evaluation revealed severe lymphocytopenia with reduced helper/suppressor t-cell ratio and impaired humoral immune response to pneumococci. extensive laboratory t ...	1997	9208237
detection of jc and bk viral genome in specimens of hiv-1 infected subjects.	human polyomaviruses jc and bk are ubiquitous in healthy human adults, persist as latent viruses and can be reactivated in the immunodeficient host giving different pathologies. due to the experimental evidence of their potential oncogenicity and neurotropism, as well as to the enhanced viral production induced by co-infection with hiv-1, a possible role of these polyomaviruses has been suggested in aids-associated progressive multifocal leucoencephalopathy (pml) and kaposi's sarcoma. jcv and bk ...	1997	9208421
kaposi's sarcoma associated herpes virus (kshv/hhv 8): epidemiology, molecular biology and tissue distribution.		1997	9208806
the kaposi's sarcoma-associated herpesvirus (human herpesvirus-8) in kaposi's sarcoma, malignant lymphoma, and other diseases.	two novel nonhuman dna fragments were discovered in an aids-related kaposi's sarcoma (ks) lesion using representational difference analysis.	1997	9209655
expression of hhv-8 latency-associated t0.7 rna in spindle cells and endothelial cells of aids-associated, classical and african kaposi's sarcoma.	analysis by polymerase chain reaction (pcr) and serological studies have demonstrated a close association between the novel human herpes virus, kaposi's sarcoma-associated herpes virus (kshv) or human herpes virus-8 (hhv-8) and the development of kaposi's sarcoma (ks). to clarify the role of hhv-8 in ks pathogenesis, we investigated at the cellular level by in situ hybridization the expression of a recently described 0.7-kb hhv-8-encoded mrna (t0.7 mrna) in ks tissues of different epidemiologica ...	1997	9212225
clastogenic factors in plasma of hiv-1 infected patients activate hiv-1 replication in vitro: inhibition by superoxide dismutase.	the frequent neoplastic disorders present in hiv-infected patients and the implication of oxidative stress in aids-kaposi's sarcoma pathogenesis prompted us to study whether the mechanisms implicated in genotoxic effects of clastogenic factors (cfs) (i.e., chromosome damaging materials released by cells under conditions of oxidant stress) can play a role in hiv-1 expression and whether exogenous superoxide dismutase can inhibit the clastogenic and hiv-inducing effects of cfs. cfs were found in t ...	1997	9215803
kaposi's sarcoma-associated herpesvirus/human herpesvirus-8: a new virus in human pathology.	the discovery of a new human herpesvirus in kaposi's sarcoma (ks) tissue of patients with aids has opened up new vistas in virology and oncology. this herpesvirus was first descriptively named ks-associated herpesvirus (kshv), but was recently renamed human herpesvirus 8 (hhv8). kshv/hhv8 dna has been found in all forms of ks, suggesting that it might be involved in the pathogenesis of ks. in addition, kshv/hhv8 can be detected in both malignant and benign lymphoproliferative disease. kshv/hhv8 ...	1997	9216532
lack of evidence of human herpesvirus 8 dna sequences in hiv-negative patients with various lymphoproliferative disorders of the skin.	human herpesvirus 8 (hhv-8) is a new virus which has been reported in kaposi's sarcoma and some lymphoproliferative disorders such as castleman's disease and body-cavity-based lymphoma. because hhv-8 shares homology with epstein-barr virus (ebv), we searched for the presence of hhv-8 dna sequences in various cutaneous t- and b-cell lymphoma by the polymerase chain reaction (pcr). forty-seven hiv-negative patients with cutaneous lymphoma or large plaque parapsoriasis were enrolled in the study. f ...	1997	9217812
simultaneous onset of primary cutaneous b-cell lymphoma and human herpesvirus 8-associated kaposi's sarcoma.	we report the simultaneous occurrence of kaposi's sarcoma (ks) and primary cutaneous b-cell lymphoma (cbcl) of the leg in a 79-year-old woman, seronegative for hiv-1, htlv-1 and htlv-2. the cbcl underwent complete clinical remission after local radiotherapy, whilst the ks became disseminated within a year following diagnosis. however, 2 years after the diagnosis of ks, the patient died with neurological symptoms. these were presumed to be due to involvement of the central nervous system by lymph ...	1997	9217827
complete sequence and genomic analysis of murine gammaherpesvirus 68.	murine gammaherpesvirus 68 (gammahv68) infects mice, thus providing a tractable small-animal model for analysis of the acute and chronic pathogenesis of gammaherpesviruses. to facilitate molecular analysis of gammahv68 pathogenesis, we have sequenced the gammahv68 genome. the genome contains 118,237 bp of unique sequence flanked by multiple copies of a 1,213-bp terminal repeat. the gc content of the unique portion of the genome is 46%, while the gc content of the terminal repeat is 78%. the uniq ...	1997	9223479
the 222- to 234-kilodalton latent nuclear protein (lna) of kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) is encoded by orf73 and is a component of the latency-associated nuclear antigen.	kaposi's sarcoma (ks)-associated herpesvirus or human herpesvirus 8 (kshv/hhv8) is the likely cause of ks and primary effusion lymphomas or body cavity-based lymphomas (bcbls). a latency-associated nuclear immunofluorescence antigen (lana) (d. h. kedes, e. operskalski, m. busch, r. kohn, j. flood, and d. ganem, nat. med. 2:918-924, 1996; s. j. gao, l. kingsley, m. li, w. zheng, c. parravicini, j. ziegler, r. newton, c. r. rinaldo, a. saah, j. phair, r. detels, y. chang, and p. s. moore, nat. med ...	1997	9223481
human herpesvirus-8-associated body cavity-based lymphoma in human immunodeficiency virus-infected patients: a unique b-cell neoplasm.	human immunodeficiency virus (hiv)-related body cavity-based lymphomas (bcbls) are known to exhibit unusual clinical, immunophenotypic, and genotypic features, and have recently been found to harbor dna sequences of a new human herpesvirus, designated kaposi's sarcoma-associated herpesvirus (kshv) or human herpesvirus 8 (hhv-8). the authors have encountered eight cases of hhv-8-associated bcbl in hiv-infected patients. a literature search revealed an additional 50 reported cases of hiv-related b ...	1997	9224748
lymphomas in patients with sjogren's syndrome are marginal zone b-cell neoplasms, arise in diverse extranodal and nodal sites, and are not associated with viruses.	the occurrence of non-hodgkin's lymphoma (nhl) is the most serious complication of sjogren's syndrome (ss). we performed a study of 16 nhls occurring in patients with an underlying ss. these lymphomas arose not only in salivary glands (7 cases) but also in other mucosal extranodal sites (the stomach [4 cases], the lung [3 cases], the skin [3 cases], the buccal mucosa [1 case], the thymus [1 case]) and in nodal sites (8 cases). low-grade marginal zone lymphomas (mzl) were diagnosed in 12 of the 1 ...	1997	9226177
hhv8 and kaposi's sarcoma: a time cohort study.	the recent finding that human herpes virus 8 (hhv8) is found in the majority of kaposi's sarcoma (ks) cases supports the epidemiological observation that the tumour may be caused by an infectious agent. this study aimed to address when and how hhv8 evolved.	1997	9231158
kaposi's sarcoma-associated herpesvirus/human herpesvirus type 8 encephalitis in hiv-positive and -negative individuals.	kaposi's sarcoma-associated herpesvirus (kshv) human herpesvirus type 8 (hhv-8) has been associated with kaposi's sarcoma and a variety of benign lymphoid proliferations including angioimmunoblastic lymphadenitis with dysproteinemia and castleman's disease. kshv/hhv-8 has also been associated with inflammatory conditions including interstitial pneumonitis. although herpesviruses are commonly associated with encephalitis in immunosuppressed individuals, kshv/hhv-8 has not previously been associat ...	1997	9233458
transplantation-associated malignancies: restriction of human herpes virus 8 to kaposi's sarcoma.	the human herpes virus 8 (hhv8) has been detected in all forms of kaposi's sarcoma. hhv8 was also reported to be present in epithelial skin tumors of patients after renal transplantation, raising the question of the clinical relevance of hhv8 in transplant-related tumors.	1997	9233723
a kaposi's sarcoma-associated herpesvirus-encoded cytokine homolog (vil-6) activates signaling through the shared gp130 receptor subunit.	the present studies analyzed the biologic activity of a gene product (vil-6) encoded by the recently discovered kaposi's sarcoma-associated herpesvirus (kshv) bearing 24.8% amino acid identity with human interleukin-6 (huil-6). based on this similarity, we hypothesized that this viral homolog might trigger the jak/stat pathway, which typically is engaged by il-6 and other cytokines. activation of receptor-associated janus tyrosine kinases (jaks) results in the subsequent phosphorylation of signa ...	1997	9235971
sequences of human herpesvirus 8 are not detected in various non-kaposi sarcoma vascular lesions.		1997	9236539
splenic vascular tumors: a histologic, immunophenotypic, and virologic study.	vascular tumors of the spleen include several different entities, some of which are unique to that organ. twenty-two such proliferations were studied, including 10 hemangiomas, six littoral cell angiomas, four angiosarcomas, and two hamartomas. the hemangiomas included seven with localized tumors and three with diffuse angiomatosis of the spleen. all cases were studied by paraffin section immunohistochemistry with a large panel of antibodies. in addition, all cases were studied for the presence ...	1997	9236839
no role for human herpes virus 8 in the etiology of infantile capillary hemangioma.	infantile capillary hemangiomas are vascular neoplasms that can appear quite infiltrative histologically and that are characterized by cords of cells with areas of marked cellularity. these lesions have been shown to contain a population of rapidly proliferative endothelial cells. given the recent association between the presence of human herpes virus 8 (hhv8) and another proliferative vascular lesion, kaposi's sarcoma, we used polymerase chain reaction technology to examine a series of 16 biops ...	1997	9237177
human herpesvirus 8 dna sequences in cd8+ t cells.		1997	9237728
[kaposi disease].		1997	9238185
epstein-barr virus latent gene expression in primary effusion lymphomas containing kaposi's sarcoma-associated herpesvirus/human herpesvirus-8.	primary effusion (body cavity-based) lymphoma (pel) is a recently recognized subtype of malignant lymphoma that exhibits distinctive clinical and biological features, most notably its usual infection with the kaposi's sarcoma-associated herpesvirus (kshv). the vast majority of cases also contain epstein-barr virus (ebv). this dual viral infection is the first example of a consistent dual herpesviral infection in a human neoplasm and provides a unique model to study viral interactions. we analyze ...	1997	9242551
[the 8th human herpesvirus].		1997	9246120
characterization of in vitro culture of hiv-negative kaposi's sarcoma-derived cells. in vitro responses to alfa interferon.	we established long-term cultures from skin tumors of nine patients suffering from classical kaposi's sarcoma (ks). spindle cells obtained after enzymatic digestion were cultured on gelatin- or fibronectin-coated flasks in dmem with 15% fetal calf serum, afgf and heparin. immunohistochemical staining was positive for mhc class i, laminin, type iv collagen, vimentin, alpha smooth muscle actin (20-40% of cells), caldesmon (20%), calponin (20-40%) and smooth muscle myosin (20-40%), and was negative ...	1997	9248622
transfusional risk of hhv-8 infection.		1997	9250205
absence of human herpes virus 8 dna sequences in large granular lymphocyte (lgl) leukemia.	the etiology of large granular lymphocyte (lgl) leukemia is uncertain. recently, a kaposi's sarcoma-associated herpes virus, denoted as human herpes virus 8 (hhv-8), has been identified. some data suggest that hhv-8 and epstein-barr virus (ebv) may interact to induce malignant transformation. infection with ebv has been implicated in the pathogenesis of some cases of lgl leukemia. therefore, we performed pcr analyses for hhv-8 detection in samples from nineteen patients with lgl leukemia; three ...	1997	9250803
the protease and the assembly protein of kaposi's sarcoma-associated herpesvirus (human herpesvirus 8).	a genomic clone encoding the protease (pr) and the assembly protein (ap) of kaposi's sarcoma-associated herpesvirus (kshv) (also called human herpesvirus 8) has been isolated and sequenced. as with other herpesviruses, the pr and ap coding regions are present within a single long open reading frame. the mature kshv pr and ap polypeptides are predicted to contain 230 and 283 residues, respectively. the amino acid sequence of kshv pr has 56% identity with that of herpesvirus salmiri, the most simi ...	1997	9261433
transmissible kaposi's sarcoma-associated herpesvirus (human herpesvirus 8) in saliva of men with a history of kaposi's sarcoma.	we have evaluated the physical state and infectious nature of kaposi's sarcoma-associated herpesvirus (kshv) in the saliva of nine persons with past or current kaposi's sarcoma (ks). kshv dna in saliva had the physical characteristics of dna present in virions. inoculation of 293 cells with cell-free saliva fluid resulted in the persistence of kshv dna in culture for at least 13 passages of the cells. the addition of tetradecanoyl phorbol acetate to kshv-infected 293 cells led to increased viral ...	1997	9261440
kaposi's sarcoma-associated herpesvirus sequences in benign lymphoid proliferations not associated with human immunodeficiency virus.	kaposi's sarcoma-associated herpesvirus (kshv) dna sequences have been identified in approximately 95% of kaposi's sarcoma (ks) lesions and primary effusion lymphomas (pels), suggesting a pathogenetic role for this virus in these lesions. however, kshv has also been identified in a variety of specimens, including lymph nodes, peripheral blood b cells, semen, and prostate tissue, with varying frequencies. this suggests that kshv, like epstein-barr virus, may be ubiquitously distributed. to evalua ...	1997	9264363
kaposi's sarcoma-associated herpesvirus (kshv): a new viral pathogen associated with kaposi's sarcoma, primary effusion lymphoma, and multicentric castleman's disease.		1997	9265864
human herpes virus 6 and human herpes virus 8 dna sequences in brains of multiple sclerosis patients, normal adults and children.	in order to determine whether the newly discovered human herpesviruses (hhvs) are involved in multiple sclerosis (ms), we investigated by polymerase chain reaction the presence of specific deoxyribonucleic acid (dna) sequences belonging to human herpesvirus 6 (hhv-6) and to human herpesvirus 8 (hhv-8), in the peripheral blood mononuclear cells (pbmcs), and in the brain and spinal cord plaques from ms patients. normal adult and stillborn children's brains were investigated as controls. pbmcs from ...	1997	9266465
role of human herpesvirus 8 in kaposi sarcoma unclear.		1997	9267258
kaposi's sarcoma as manifestation of immunosuppression in organ transplant recipients.	we report 2 patients, negative for human immunodeficiency virus (hiv) antibodies, who developed generalized kaposi's sarcoma (ks), one 5 months after renal transplantation while receiving cyclosporin a and prednisone, and the other 24 months after heart transplantation while receiving cyclosporin a, azathioprine and prednisone. reduction of the immunosuppressive therapy combined with chemotherapy and radiotherapy in both cases led to a remission of ks without development of transplant rejection.	1997	9267755
a patient with classical kaposi's sarcoma and angiosarcoma: bad luck or a common aetiology?	classical kaposi's sarcoma and angiosarcoma are rare malignant tumours arising from vascular tissue. however, they have distinct histological features and appear also to differ in their epidemiology and pathogenesis. we present the case history of a patient in whom both these tumours occurred simultaneously. the relationship between these events is discussed in the light of the new virus, human herpes virus 8.	1997	9269554
[human herpesvirus 8. a kaposi sarcoma-associated herpesvirus?].		1997	9273753
absence of human herpesvirus-8 dna in angiosarcomas and other skin tumours in immunocompetent patients, and in graft-versus-host disease in the immunosuppressed recipients of bone marrow transplants.		1997	9274652
a broad-spectrum chemokine antagonist encoded by kaposi's sarcoma-associated herpesvirus.	kaposi's sarcoma-associated herpesvirus encodes a chemokine called vmip-ii. this protein displayed a broader spectrum of receptor activities than any mammalian chemokine as it bound with high affinity to a number of both cc and cxc chemokine receptors. binding of vmip-ii, however, was not associated with the normal, rapid mobilization of calcium from intracellular stores; instead, it blocked calcium mobilization induced by endogenous chemokines. in freshly isolated human monocytes the virally en ...	1997	9287217
[detection of human herpesvirus 8 (hhv 8) in kaposi sarcomas of the gastrointestinal tract].	involvement of kaposi's sarcoma in the gastrointestinal tract is common in aids patients. the disease is, however, usually asymptomatic and, due to the tumor growth primarily in the submucosa, biopsy diagnosis is possible in under 25%. the recently described human herpes virus 8 (hhv8) is closely associated with all forms of kaposi's sarcoma. detection of hhv8 in the tissue samples may therefore improve the diagnosis of gastrointestinal kaposi's sarcoma. in the present study we analyze autopsy s ...	1997	9289827
[human herpesvirus 8 (kaposi's sarcoma-associated herpesvirus].		1997	9290315
cancer incidence among an hiv-infected cohort. pulmonary complications of hiv infection study group.	malignancies, particularly kaposi's sarcoma and non-hodgkin's lymphoma (nhl), are associated with human immunodeficiency virus (hiv) infection. cancer incidence among 1,073 asymptomatic hiv-infected individuals from the pulmonary complications of hiv infection study cohort, persons from six states followed from 1988 to 1994, was examined. total cancer incidence was 3.99/100 person-years; for kaposi's sarcoma, incidence was 2.64 cases/100 person-years, and for nhl, it was 1.18 cases/100 person-ye ...	1997	9290507
lymphoma of the extrahepatic biliary ducts in acquired immunodeficiency syndrome.	gastrointestinal neoplasia in acquired immunodeficiency syndrome (aids) frequently presents as a non-hodgkin's lymphoma or kaposi's sarcoma, at sites including the oral cavity, esophagus, small bowel, and mesenteries. primary lymphoma of the biliary ducts, even in the setting of aids, is rare. this patient, an older male with no preoperatively known aids risk factors, presented with painless jaundice and radiographic findings of focal hepatic duct stenosis, suggesting either a klatskin tumor or ...	1997	9290526
human herpesvirus 8 infection occurs following adolescence in the united states.	most recent evidence suggests that human herpesvirus 8 (hhv-8) infection is restricted to persons with kaposi's sarcoma (ks) or to persons who may subsequently develop ks. to accurately determine the prevalence of infection in the united states, children and adults with aids were examined for evidence of hhv-8 infection to see whether hhv-8 (like other herpesviruses) would be readily detected in immunosuppressed persons. by use of nested polymerase chain reaction, dna specific for hhv-8, epstein ...	1997	9291330
absence of detectable human herpesvirus 8 in the semen of human immunodeficiency virus-infected men without kaposi's sarcoma.	the prevalence of human herpesvirus 8 (hhv-8)/kaposi's sarcoma (ks)-associated herpesvirus was investigated in the semen of 99 human immunodeficiency virus (hiv)-infected men (median cd4 cell count, 357/mm3) by use of a polymerase chain reaction (pcr) assay capable of detecting <10 copies of hhv-8 dna. of the subjects, 95 (96%) self-identified as men who have sex with men (msm), and 3 had a history of clinical ks. seminal cell specimens were negative for hhv-8 in 98 subjects. none of the 26 with ...	1997	9291331
high-dose interferon-alpha2a exerts potent activity against human immunodeficiency virus type 1 not associated with antitumor activity in subjects with kaposi's sarcoma.	anti-human immunodeficiency virus type 1 (hiv-1) activity was assessed in hiv-1-infected homosexual and bisexual men receiving 18-36 miu/day of recombinant interferon (ifn)-alpha2a for kaposi's sarcoma (ks). the median baseline hiv-1 rna level was 4.99 log10 copies/ml. seventeen subjects (68%) showed an rna decline > or = .5 log10/ml, with a maximum at week 4 (median decline = 1.91, range = 3.64-1.15; p = .0007), after which rna levels stabilized. eight subjects (32%) with lower median initial c ...	1997	9291341
prevalence of human herpesvirus-8 in skin lesions.	a putative new herpesvirus, designated kaposi's sarcoma-associated herpesvirus (kshv) or human herpesvirus-8 (hhv-8) was recently identified in aids-related kaposi's sarcoma. the dna sequence of the virus is homologous to the gamma-herpesviruses such as herpesvirus saimiri or epstein-barr virus, both of which are tumorigenic, suggesting that this new virus may also be oncogenic. thus, we determined whether the virus is present in other skin lesions. dna samples from 118 skin specimens were teste ...	1997	9292063
aids-related kaposi's sarcoma: prospective validation of the aids clinical trials group staging classification. aids clinical trials group oncology committee.	to prospectively validate the aids clinical trials group (actg) staging classification for aids-associated kaposi's sarcoma (ks).	1997	9294471
human immunodeficiency virus (hiv)-related pulmonary complications in pregnancy.	with changes in the demographics of human immunodeficiency virus (hiv) infection, women and children are becoming the fastest growing group of newly infected patients. with longer survival after hiv infection, more women infected with hiv are becoming pregnant. pulmonary disease is one of the most common presenting conditions in an aids-defining illness. pneumocystis carini pneumonia and tuberculosis are the most common disorders that herald the onset of aids. they are also the most frequently e ...	1997	9298723
detection of human herpesvirus 8 dna sequences in peripheral blood mononuclear cells of children.	human herpesvirus 8 (hhv-8) dna sequences were examined in peripheral blood mononuclear cell (pbmc) dna samples of 56 children, 15 healthy adults, and 10 renal transplant patients by the polymerase chain reaction (pcr). the pcr amplification was carried out using the published ks330(233) primer pairs to amplify hhv-8 dna sequences. the pcr-amplified products were confirmed by southern blot hybridization with radiolabeled 233 bp hhv-8 dna fragment, which was cloned and sequenced from the pcr-ampl ...	1997	9298737
the structure and coding organization of the genomic termini of kaposi's sarcoma-associated herpesvirus.	kaposi's sarcoma-associated herpesvirus (kshv) is a novel human herpesvirus closely linked to two aids-related neoplasms. we have prepared dna from kshv virions produced in cell culture and have examined the structure of the viral genomic termini. as in the related simian herpesvirus h. saimiri (hvs), the central unique region of kshv dna is bounded by tandemly repeated units of noncoding, gc-rich dna. the kshv repeats are 803 bp in length and are 85% g+c. each molecule harbors 35-45 such repeat ...	1997	9299627
human herpesvirus 8 and kaposi's sarcoma.	in the last 2 years, the discovery that the suspected causative agent of kaposi's sarcoma (ks) is a new gamma-herpesvirus, called human herpesvirus type 8 (hhv8) or kaposi's sarcoma-associated herpesvirus (kshv), has been followed by studies showing it to be a sine qua non of all clinical forms of ks and a specific marker for ks in the differential diagnosis of angioproliferative lesions. reports that the virus is ubiquitous have been based on the polymerase chain reaction and appear to be contr ...	1997	9300629
[severe complications, adverse effects due to blood transfusion and its measures--transfusion transmitted viral infections].	since the implementation of blood screening for hbs-ag, htlv-1-ab, hiv-ab and hcv-ab, donor blood collected by the japan red cross blood center has become among the safest in the world. the current incidence of hbv, htlv-1, hiv and hcv transmission by blood transfusion are estimated to be 1/500,000, 1/50,000, 1/1,000,000 and 1/500, respectively. even though the latest, most advanced techniques are used, it is impossible to completely prevent transmission of these viruses. hiv transmission from d ...	1997	9301297
in vitro antiviral drug sensitivity of the kaposi's sarcoma-associated herpesvirus.	kaposi's sarcoma-associated herpesvirus (kshv), or human herpesvirus 8, has been implicated as the causative agent of kaposi's sarcoma. retrospective studies show that the risk of development of kaposi's sarcoma is significantly lower in aids patients who received ganciclovir or phosphonoformic acid (pfa) therapy. therefore, in vitro antiviral drug sensitivity of kshv was studied.	1997	9302441
no effect of protease inhibitor on clinical and virological evolution of castleman's disease in an hiv-1-infected patient.		1997	9302455
kaposi's sarcoma--associated herpesvirus: a newly recognized pathogen.		1997	9305454
primary plasmacytoma of lymph nodes.	primary plasmacytoma of the lymph nodes is very rare, and there are fewer than 20 reported cases. these cases appeared to have a better prognosis than other extramedullary plasmacytomas, with rare recurrence and no progression to myeloma after treatment. to better characterize the clinicopathological features and the pathogenesis of primary plasmacytoma of the lymph nodes, we reviewed our consultation files and retrieved seven such cases. the age of presentation ranged from 39 to 76 years (media ...	1997	9308734
malignant tumors and aids.	one in six patients with acquired immunodeficiency syndrome (aids) both in the usa and europe develop malignancies, in particular kaposi's sarcoma (ks) and non-hodgkin's lymphoma (nhl). after an initial rapid increase, the proportion of aids patients with ks steadily declined in the usa and in europe, while the proportion of aids-nhl has been stable during the last decade in the usa and europe. human immunodeficiency virus (hiv) infected patients are living longer due to advances in antiretrovir ...	1997	9309244
[recent data on the epidemiology of non-hodgkin lymphoma. groupe d'etudes des lymphomes de l'adulte (gela)].	non-hodgkin's lymphoma is a common condition whose incidence has increased by 75% over the last 15 years. the hiv epidemic is among the factors that have contributed to this increase: patients with aids have 1% annual risk of developing non-hodgkin's lymphoma, and the increase in survival of aids patients has led to an increase in the frequency of aids-associated lymphomas. a number of other viruses are directly involved in the occurrence of lymphomas, such as the htlv-1 and the ebv. the hsv typ ...	1997	9309258
effect of dna synthesis inhibitors on kaposi's sarcoma-associated herpesvirus cyclin and major capsid protein gene expression.	epidemiological studies strongly suggest that a newly discovered herpesvirus, kaposi's sarcoma associated-herpesvirus (kshv/hhv8), is the likely infectious cause of kaposi's sarcoma (ks). identification of this agent suggests the possibility that existing anti-herpesvirus chemotherapeutics have activity against kshv. using kshv/epstein-barr virus (ebv)-coinfected cell line bc-1 and kshv-infected/ebv-negative cell line bc-3, the effect of dna polymerase inhibitors in the presence of virus-inducin ...	1997	9310290
monocytes in kaposi's sarcoma lesions are productively infected by human herpesvirus 8.	pcr analysis and serological studies demonstrated a close association between kaposi's sarcoma (ks)-associated herpesvirus, or human herpesvirus 8 (hhv-8), and the development of kaposi's sarcoma (ks). the majority of the ks cells were shown to be latently infected by the virus. in this study we investigated which type of cell is productively infected in ks lesions. in situ hybridization was performed with strand-specific rna probes complementary to the sequences coding for the minor capsid prot ...	1997	9311888
human herpesvirus 8 strain variability in clinical conditions other than kaposi's sarcoma.		1997	9311909
human herpesviruses at sixes, sevens, and more.		1997	9313006
herpesvirus-like dna sequences in kaposi's sarcoma lesions from two women with aids.		1997	9314474
angiogenic and hiv-inhibitory functions of kshv-encoded chemokines.	unique among known human herpesviruses, kaposi's sarcoma-associated herpesvirus (kshv or hhv-8) encodes chemokine-like proteins (vmip-i and vmip-ii). vmip-ii was shown to block infection of human immunodeficiency virus-type 1 (hiv-1) on a cd4-positive cell line expressing ccr3 and to a lesser extent on one expressing ccr5, whereas both vmip-i and vmip-ii partially inhibited hiv infection of peripheral blood mononuclear cells. like eotaxin, vmip-ii activated and chemoattracted human eosinophils b ...	1997	9323208
occurrence of human herpes virus 8 in kaposi's sarcoma and other tumours in south africa.		1997	9323419
risk of kaposi's sarcoma-associated herpes virus transmission from donor allografts among italian posttransplant kaposi's sarcoma patients.	kaposi's sarcoma-associated herpesvirus (kshv) is a newly discovered herpes virus found in all forms of kaposi's sarcoma (ks) including ks among immunosuppressed transplant patients. it is unknown whether this virus is transmitted by organ transplantation or is reactivated during immunosuppression among those patients infected before transplantation. to investigate the risk of kshv transmission during organ transplantation, we conducted a case-control study of transplant recipients with and with ...	1997	9326251
human herpesvirus-8.	our current understanding of kaposi's sarcoma (ks) has evolved from initial descriptions of this "idiopathic sarcoma" to a progressively detailed characterization of its probable infectious origin. this article traces the explosive discovery of human herpesvirus-8 (hhv-8) and its etiologic associations with ks, castleman's disease, and primary effusion lymphomas. a framework for understanding how viral infection leads to development of these unusual disease is presented. the possible role of hhv ...	1997	9327222
[intestinal diseases in hiv infection].	the gastrointestinal tract is very frequently affected by the manifestations of the acquired immunodeficiency syndrome (aids). a variety of opportunistic viral, fungal, bacterial, protozoal and helmintic infections and different unusual malignancies such as kaposi's sarcoma, non-hodgkin's lymphoma and papilloma-virus associated anal cancer are responsible for much of the morbidity and mortality in aids. because specific therapy is not always available, in particular diagnosis of potentially infe ...	1997	9333912
hhv-8 in pbmc and kaposi's sarcoma activity.		1997	9334874
human herpesvirus-8 (hhv-8) dna associated with anaplastic large cell lymphoma of the b-cell type in an hiv-1-positive patient.		1997	9335459
lack of serologic evidence for involvement of human herpesvirus 8 in autoimmune diseases.		1997	9336431
preventing and treating major opportunistic infections in aids. what's new and what's still true.	new highly active antiretroviral therapies are boosting the blood absolute cd4+ counts of many patients with aids and are decreasing the prevalence of aids-related opportunistic infections. nevertheless, the prevention, diagnosis, and treatment of opportunistic infections remain important features of management of hiv infection. in recent years, significant advances have been made in the prevention and treatment of opportunistic diseases such as pneumocystis carinii pneumonia, cytomegalovirus re ...	1997	9336601
quantitative polymerase chain reaction for human herpesvirus diagnosis and measurement of epstein-barr virus burden in posttransplant lymphoproliferative disorder.	human herpesviruses can cause acute diseases such as chicken pox or mononucleosis, but also may reactivate during immunosuppression and result in severe or life-threatening illnesses such as shingles or lymphoproliferative disorders. we report the development and validation of a quantitative pcr method to measure viral burden for all eight human herpesviruses (hsv1, hsv2, vzv, ebv, cmv, hhv6, hhv7, and kshv) in patients' samples. the method uses an internal standard that is coamplified with the ...	1997	9342002
human herpesvirus dna in prostate and semen from hiv-negative individuals in italy.		1997	9342083
bovine herpesvirus 4 borfe2 protein inhibits fas- and tumor necrosis factor receptor 1-induced apoptosis and contains death effector domains shared with other gamma-2 herpesviruses.	fas- and tumor necrosis factor receptor 1 (tnfr1)-induced apoptosis is mediated by the interaction of fadd with caspase-8. here, we report that the bovine herpesvirus 4 (bhv4) borfe2 gene encodes a protein that inhibits fas- and tnfr1-induced apoptosis and contains death effector domains (deds). using the yeast two-hybrid system, we found that the borfe2 protein interacts with the prodomain of caspase-8. furthermore, we show that bhv4 borfe2 is a member of a family of ded-containing proteins tha ...	1997	9343261
hhv-8 and multiple myeloma in france.		1997	9343508
hhv-8 and multiple myeloma in the uk.		1997	9343509
resolution of kaposi's sarcoma associated with undetectable level of human herpesvirus 8 dna in a patient with aids after protease inhibitor therapy.		1997	9344209
the epidemiology of soft tissue sarcoma.	soft tissue sarcoma (sts) accounts for approximately 1% of all cancers diagnosed annually in the united states. population-based data from connecticut covering the years 1935-1989 have shown an increasing incidence of sts in both genders, with a greater increase among men than women. the recent increase in acquired immune deficiency syndrome-related kaposi's sarcoma does not explain the upward trend in sts, dating back decades. etiologic heterogeneity is suggested by epidemiologic variations tha ...	1997	9344316
kshv and kaposi's sarcoma: the end of the beginning?		1997	9346233
kaposi's sarcoma presenting as a protracted multisystem illness in an adolescent liver transplant recipient.	kaposi's sarcoma (ks) is a common malignancy in patients with acquired immunodeficiency syndrome (aids), classically appearing as red to purple plaques containing small papules and nodules. we report our experience with an adolescent orthotopic liver transplant recipient who presented with an unusual presentation of ks. the patient had a protracted multisystem illness that began with hemolytic anemia, fevers, and fatigue and progressed to pancreatitis, sinusitis, lymphadenopathy, and mouth ulcer ...	1997	9346799
viral gene expression and pathogenesis in three emerging diseases: hiv and aids; htlv-i and ham/tsp; and hhv-8 and kaposi's sarcoma.		1997	9348166
kaposi's sarcoma-associated herpesvirus (kshv)/human herpesvirus 8 (hhv8)--a new human tumour virus.	recently, a novel dna virus has been molecularly cloned from kaposi's sarcoma (ks) tissue, a tumour common in acquired immune deficiency syndrome (aids). analysis of the viral genome confirms that it is a relative of human herpesviruses and the virus has been designated hhv-8. epidemiological evidence suggests a strong aetiological link between the presence of hhv-8 dna and/or antibodies against the virus, and ks. additional sequence analysis suggests that the hhv-8 genome contains sequences whi ...	1997	9349227
ultrastructural characterization of human herpesvirus 8 (kaposi's sarcoma-associated herpesvirus) in kaposi's sarcoma lesions: electron microscopy permits distinction from cytomegalovirus (cmv).	kaposi's sarcoma (ks) has been shown by molecular techniques to be associated with infection with human herpesvirus 8 (hhv8/kshv), but specific ultrastructural characterization of the virus has been impaired by the frequent presence in these lesions of other herpesviruses, particularly cytomegalovirus (cmv). since the ultrastructural appearance of hhv8/kshv has been studied in the cell line ks-1 uninfected with other viruses including cmv, it was possible to undertake a comparative study of cmv ...	1997	9349229
human herpesvirus 8 dna sequences in angiosarcoma of the face.		1997	9349352
kaposi's sarcoma and the new herpesvirus.		1997	9350198
absence of human herpesvirus 8 dna in benign and malignant endothelial lesions.		1997	9350784
histopathologic findings in the lymphoid and reticuloendothelial system in pediatric hiv infection: a postmortem study.	the present report describes the histopathological features of lymphoreticular tissues in 29 pediatric autopsies of human immunodeficiency virus (hiv)-infected patients. mean age for the whole group was 1.77 years; 68.9% and 62% of the cases were 2 years old or less and 1 year old or less at the time of death, respectively. twenty-one cases were categorized as acquired immunodeficiency syndrome (aids) and the rest included seven hiv-positive newborns and infants and two infants belonging to a hi ...	1997	9353825
hiv-associated human herpesvirus 8-positive primary lymphomatous effusions: radiologic findings in six patients.	to define the imaging features of body cavity-based lymphoma (bcbl) related to the acquired immunodeficiency syndrome. this is a peculiar type of non-hodgkin lymphoma harboring infection by human herpesvirus 8 (hhv-8) and displaying a peculiar tropism for the serous body cavities.	1997	9356629
follicular dendritic cell sarcoma complicated by hyaline-vascular type castleman's disease in a schizophrenic patient.	follicular dendritic cell (fdc) sarcoma is an exceedingly rare neoplasm of unknown pathogenesis. a case of fdc sarcoma complicated by the hyaline-vascular type castleman's disease occurring in a schizophrenic male is presented. swelling of the left cervical lymph node appeared in a 44-year-old male schizophrenic who had been medicated with major tranquilizers for 20 years. he had had a history of cervical lymphadenopathy 14 years before, for which a diagnosis of hyaline-vascular type castleman's ...	1997	9361105
study of prevalence, severity, and etiological factors associated with acute pancreatitis in patients infected with human immunodeficiency virus.	patients with the human immunodeficiency virus (hiv) disease can develop pancreatic gland inflammation from hiv infection and related causes, or from factors totally independent of it. the incidence and severity acute pancreatitis in patients with hiv diseases and the frequency of associated etiological factors have not been examined in any detail. the purpose of this study was to (a) determine the prevalence of acute pancreatitis, (b) evaluate severity of pancreatic gland inflammation, (c) iden ...	1997	9362189
human herpesvirus 8 in kaposi's sarcoma of an hiv-negative japanese woman with adult t-cell leukemia.		1997	9163629
clinical and pharmacokinetic study of oral etoposide in patients with aids-related kaposi's sarcoma with no prior exposure to cytotoxic therapy.	in this phase ii and pharmacokinetic study, chronic, low-dose, oral etoposide was evaluated for its efficacy in patients with aids-related kaposi's sarcoma who were not previously exposed to cytotoxic therapy.	1997	9164226