Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| study of interleukin-10 and interleukin-12 productions in response to lipopolysaccharides extracted from two different brucella strains. | the aim of the present study is to investigate the cytokines induction by smooth lipopolysaccharides (s-lps) extracted from brucella melitensis (rev1 vaccine strain) and brucella abortus (a field isolate). these lipopolysaccharides were used to induce inflammatory cytokines production in peripheral blood cell culture of healthy individuals. secretion of il-10 and il-12 (p70) were measured by means of specific elisa. in addition, intracellular expression of il-12 was assessed in cd14+ cells by fl ... | 2002 | 11848132 |
| analysis of sampling strategies to substantiate freedom from disease in large areas. | in this paper, we deal with the strategies of surveys to substantiate freedom from disease for a certain territory. infection might not be distributed homogeneously. so, a relatively high within-herd prevalence might be observed while the herd-level prevalence is lower. for this situation, we compare various two-stage sample strategies. the calculation of appropriate sample sizes becomes quite complicated. the theoretical generalization of the hypergeometric distribution by cameron and baldock [ ... | 2002 | 11849726 |
| biological warfare: implications for antimicrobial use. | biological warfare is intended to incapacitate a large number of individuals at a single exposure, creating epidemic-type disease, death, and social chaos. the organisms with potential for immediate use as bacteriologic weapons are bacillus anthracis, brucella melitensis, yersinia pestis, and vibrio cholera, all necessitating antibiotic therapy for a cure. it is reasonable, therefore, to assume that a biological attack, or even a hoax, would requiure thousands of individuals over a large area to ... | 2002 | 11853654 |
| immunogenicity of the brucella melitensis recombinant ribosome recycling factor-homologous protein and its cdna. | a study was conducted to evaluate the immunogenicity of the brucella melitensis ribosome recycling factor (rrf)-homologous protein (cp24). the cp24 gene was cloned, expressed in escherichia coli and purified. the resulting purified recombinant protein (rcp24) produced delayed-type hypersensitivity (dth) reactions in b. melitensis-infected mice but not in naive controls. thus, we decided to characterise the immune responses generated with dna vaccination (pcdnacp24) or immunisation with the rcp24 ... | 2002 | 11858876 |
| isolation of brucella melitensis from an arabian oryx (oryx leucoryx). | 2002 | 11888113 | |
| brucellosis in the etiology of febrile neutropenia: case report. | brucellosis is one of the leading diseases in the differential diagnosis of fever of unknown origin in some parts of the world. it can lead to treatment failure because of slow growth in blood cultures and late appearance of signs and symptoms in patients with febrile neutropenia who were unresponsive to empirical antibiotic treatment. during the last year in our oncology unit adjuvant chemotherapy was given to 3 patients with breast (n=1) and stomach cancer (n=2) and febrile neutropenia was see ... | 2002 | 11892906 |
| splenic infarction associated with acute brucella mellitensis infection. | 2002 | 11913506 | |
| identification of the brucella melitensis vaccine strain rev.1 in animals and humans in israel by pcr analysis of the psti site polymorphism of its omp2 gene. | adverse effects of strain persistence and secretion in milk have been encountered with the brucella melitensis vaccine strain rev.1. field isolates obtained from vaccinated animals and from a human resembled the vaccine strain rev.1 by conventional bacteriological tests. the lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirmation of the homology of the rev.1-like field isolates to the vaccine strain. the composition of the omp ... | 2002 | 11923376 |
| medically treated splenic abscess due to brucella melitensis. | brucellosis may lead to complications that affect different organ systems, including the liver and spleen. in acute disease, hepatosplenic abscess is a rare complication. we report herein a woman with splenic abscess due to acute brucella melitensis infection who was successfully treated with antibiotics alone. | 2002 | 11928846 |
| chronic hepatosplenic abscesses in brucellosis. clinico-therapeutic features and molecular diagnostic approach. | in order to analyze the clinical and therapeutic features of chronic hepatosplenic abscesses, and to define the diagnostic yield of new molecular techniques, we describe seven cases, four hepatic and three splenic, of this uncommon complication of brucellosis. onset of symptoms in all cases was insidious and the diagnostic delay considerable. abdominal ct scan showed large, poorly defined lesions, with heterogeneous attenuation and thick central calcifications surrounded by hypointense areas. hi ... | 2002 | 11929686 |
| combined brucella melitensis and streptococcal viridans endocarditis. | infectious endocarditis is a potentially lethal inflammation of the hearts' inner lining invaded by microorganisms. the mortality from this illness increases as the number of infective organisms rises to 2, due to involvement of the left side of the heart. these microorganisms usually arise from the patient's own flora but can be acquired from the environment. fever and heart murmurs are the principal clinical manifestations followed by a plethora of peripheral signs due to dissemination of micr ... | 2002 | 11938375 |
| brucellosis as a cause of septic loosening of total hip arthroplasty. | infection after a total hip arthroplasty is a severe complication and is associated with a high incidence of morbidity. we describe a case of late infection, 5 years after the implantation of a cemented hip prosthesis. the infection was caused by gram-negative brucella melitensis and occurred in a 63-year-old man who owned cattle. as far as we know, such a complication has never been published before. | 2002 | 11938520 |
| [analysis of immune response of guinea pigs infected with brucella melitensis]. | the dynamics of the first antigen specific stage of immune response to brucella infection was experimentally studied with the method of binding adsorbed antigenic immunoreagents with lymphocytes. the study revealed that the content of antigen-binding lymphocytes (abl) reached its maximum as early as on day 7 after infection, gradually decreasing afterwards (but even on day 90 abl could be detected in the blood). the specificity of abl was proved by the fact that they were absent in noninfected a ... | 2002 | 11949255 |
| brucella melitensis endocarditis of ventricular septal defect patch: successful surgical treatment without replacement. | 2003 | 12539006 | |
| nramp1 is not a major determinant in the control of brucella melitensis infection in mice. | brucella, the causative agent of brucellosis in animals and humans, can survive and proliferate within macrophages. macrophages mediate mouse resistance to various pathogens through the expression of the nramp1 gene. the role of this gene in the control of brucella infection was investigated. when balb/c mice (nramp1(s)) and c.cb congenic mice (nramp1(r)) were infected with brucella melitensis, the number of brucella organisms per spleen was significantly larger in the c.cb mice than in the balb ... | 2003 | 12540538 |
| biosynthesis of phosphatidylcholine in bacteria. | phosphatidylcholine (pc) is the major membrane-forming phospholipid in eukaryotes and can be synthesized by either of two pathways, the methylation pathway or the cdp-choline pathway. many prokaryotes lack pc, but it can be found in significant amounts in membranes of rather diverse bacteria and based on genomic data, we estimate that more than 10% of all bacteria possess pc. enzymatic methylation of phosphatidylethanolamine via the methylation pathway was thought to be the only biosynthetic pat ... | 2003 | 12547654 |
| optimum detection times for bacteria and yeast species with the bactec 9120 aerobic blood culture system: evaluation for a 5-year period in a turkish university hospital. | we tracked and documented the time of positivity of blood cultures by using the bactec 9120 (becton dickinson diagnostic instrument systems) blood culture system over a 5-year study period. a 7-day protocol of the incubation period was selected, and a total of 11156 blood cultures were evaluated. the clinically significant microorganisms (32.95%) were isolated in 3676 specimens. gram-positive and -negative bacterial isolation rates were found to be 41.07 and 44.88%, respectively. yeasts were fou ... | 2003 | 12574291 |
| serological diagnosis of brucellosis caused by brucella canis. | blood serum samples from 2,328 dogs were tested to detect antibodies against brucella canis with the agar gel immunodiffusion (agid) and 2-mercaptoethanol slide agglutination test (me-sat) using brucella ovis as the antigen. all blood serum samples were also evaluated for antibodies against brucella abortus and brucella melitensis using the rose bengal test. twentyfive (1.07%) of the sera evaluated were considered positive with agid test. only 4 (16%) of these blood serum samples were positive w ... | 2003 | 12578313 |
| epidemiological, clinical and laboratory features of brucellosis: a prospective evaluation of 120 adult patients. | this prospective study was carried out in the department of infectious diseases of gaziantep university between january 1997 and december 1999 to evaluate the epidemiological, clinical and laboratory features of brucellosis in south-eastern turkey. one hundred and twenty consecutive patients with active brucellosis were enrolled. the commonest way of transmission was ingestion of milk products from diseased animals. brucella melitensis was isolated in the specimens of 31 (45.5%) of 68 patients. ... | 2003 | 12587937 |
| comparison of polymerase chain reaction and bacteriological culture for the diagnosis of sheep brucellosis using aborted fetus samples. | pcr assay has been shown to be a promising option for the diagnosis of brucellosis. however, few studies have been performed with field samples in order to evaluate the assay as a diagnostic tool. in this study, routine use of a species-specific pcr assay previously developed for the identification of brucella cultures was assessed for the detection of brucella dna directly from the stomach contents of aborted sheep fetuses. the assay is based on the insertion sequence is711 in the brucella chro ... | 2003 | 12591206 |
| production of the type iv secretion system differs among brucella species as revealed with virb5- and virb8-specific antisera. | expression of the virb operon, encoding the type iv secretion system required for brucella suis virulence, occurred in the acidic phagocytic vacuoles of macrophages and could be induced in minimal medium at acidic ph values. to analyze the production of virb proteins, polyclonal antisera against b. suis virb5 and virb8 were generated. western blot analysis revealed that virb5 and virb8 were detected after 3 h in acidic minimal medium and that the amounts increased after prolonged incubation. unl ... | 2003 | 12595417 |
| antibodies to the cp24 protein of brucella melitensis lack diagnostic usefulness in ovine brucellosis. | the potential diagnostic usefulness of antibodies to the ribosome recycling factor of brucella melitensis (cp24) was assessed in sheep by an indirect elisa with purified recombinant cp24. sera from uninfected animals from the uk (n=44) and from local flocks (n=42), from sheep naturally infected with b. melitensis (n=12) or b. ovis (n=12), and from lambs (n=7) or pregnant ewes (n=6) vaccinated with b. melitensis rev-1, were assayed. high specific optical densities (od(with antigen) - od(without a ... | 2003 | 12636998 |
| the recombinant omp31 from brucella melitensis alone or associated with rough lipopolysaccharide induces protection against brucella ovis infection in balb/c mice. | immunogenicity and protective activity against brucella ovis of detergent-extracted recombinant omp31 (romp31 extract) from brucella melitensis produced in escherichia coli, purified rough lipopolysaccharide from b. ovis (r-lps) and a mixture of romp31 extract and r-lps (romp31 extract + r-lps) were assessed in balb/c mice. the experimental vaccines were compared with a hot saline extract (hs extract) from b. ovis mainly composed of outer membrane proteins (omps) and r-lps, and known to be prote ... | 2003 | 12650766 |
| brucella prosthetic joint infection: a report of 3 cases and a review of the literature. | we report 3 cases of brucella melitensis infection of prosthetic hips and knees, and we summarize data about 4 cases reported in the literature. six of the 7 affected patients were men. the median duration from prosthesis implantation to the onset of symptoms was 38.7 months. five patients had only local symptoms. preoperative joint aspirates yielded negative culture results for 3 patients, and blood culture results were negative for 6 patients. excisional arthroplasty was the initial interventi ... | 2003 | 12652405 |
| therapeutic effect of spiramycin in brucellosis. | this study was undertaken to investigate the usefulness of spiramycin in treatment for brucellosis in an animal model. | 2003 | 12654065 |
| interactions between brucella melitensis and human phagocytes: bacterial surface o-polysaccharide inhibits phagocytosis, bacterial killing, and subsequent host cell apoptosis. | brucellae are gram-negative intracellular pathogens that survive and multiply within host phagocytic cells. smooth organisms present o-polysaccharides (ops) on their surface. the wboa gene, which codes for the enzyme glycosyl transferase, is essential for the assembly of o-chain in brucella. deletion of wboa in smooth, virulent b. melitensis 16m results in a rough mutant designated wrr51. unlike b. abortus, both smooth and rough strains of b. melitensis are resistant to complement-mediated killi ... | 2003 | 12654833 |
| brucella melitensis infection stimulates an immune response leading to kikuchi-fujimoto disease. | a 43-year-old greek cattler with a history of brucellosis three months previously for which he was treated with sulfonamides for three weeks, was admitted to the hospital complaining of fever, arthralgias, night sweats, painful cervical and axillary lymph nodes as well as a weight loss of 8 kg in the previous four months. since microbiological and serological studies did not give a specific diagnosis, an open cervical lymph node biopsy was performed. the histological examination revealed kikuchi ... | 2003 | 12655790 |
| [maculopapular eruption with fluctuating fever]. | 2003 | 12671590 | |
| brucella melitensis bacteremia in children: review of 62 cases. | brucella bacteremia is not uncommon in children living in endemic areas. reports on brucella bacteremia, however, are scarce. its clinical features and complications are unknown. this retrospective review describes the clinical and laboratory characteristics, the relapse rate, and response to different regimens of antimicrobials in children with brucella bacteremia over a 5-year period. antimicrobial susceptibility testing was performed on all isolates. data on 62 children with brucella bacterem ... | 2003 | 12678419 |
| [subphrenic abscess during the course of acute brucellosis: diagnosis and treatment with percutaneous sonographically-guided drainage]. | abscess formation during the course of acute brucellosis is a rare event. a case of subphrenic abscess, the first to our knowledge, is described. a 49 years-old male patient with fever and a mild increase in alt and gamma-gt was referred to our institution. routine blood exams tested negative and antibodies against brucella spp. were also negative. ct examination of abdomen was normal. after 8 days, us examination showed a liquid area under the right diaphragma and us-guided puncture revealed an ... | 2003 | 12719669 |
| misidentification of brucella melitensis as ochrobactrum anthropi by api 20ne. | 2003 | 12721322 | |
| [brucellosis]. | 2003 | 12722260 | |
| type iii secretion homologs are present in brucella melitensis, b. ovis, and b. suis biovars 1, 2, and 3. | protein sequences from characterized type iii secretion (tts) systems were used as probes in silico to identify several tts gene homologs in the genome sequence of brucella suis biovar 1 strain 1330. four of the genes, named flhb, flip, flir, and flif on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in pcr and hybridization assays to determine their distribution among other brucella nomen species and biovars. the results indicated that flhb, flip, flir and ... | 2003 | 12732970 |
| isolation and expression of recombinant antibody fragments to the biological warfare pathogen brucella melitensis. | brucella melitensis is a highly infectious animal pathogen able to cause a recurring debilitating disease in humans and is therefore high on the list of biological warfare agents. immunoglobulin genes from mice immunized with gamma-irradiated b. melitensis strain 16m were used to construct a library that was screened by phage display against similarly prepared bacteria. the selected phage particles afforded a strong enzyme-linked immunosorbent assay (elisa) signal against gamma-irradiated b. mel ... | 2003 | 12738372 |
| spontaneous bacterial peritonitis due to brucella melitensis. | peritonitis is an extremely rare complication of brucellosis. a case is reported of blood and ascitic culture-proven spontaneous bacterial peritonitis caused by brucella melitensis, in a patient who had also cirrhosis. | 2003 | 12751716 |
| acute abdomen due to brucella melitensis. | a case of acute abdomen caused by a brucella melitensis is reported. the patient presented with biliary involvement in the form of acute acalculous cholecystitis and developed acute appendicitis that resulted in his surgical treatment. | 2003 | 12751720 |
| [sarcoidosis and brucellosis: a strange and infrequent association]. | a cattlewoman, a 63-year-old patient with sarcoidosis associated to mellitensis brucellosis, was studied. clinical manifestations of both multisystemic diseases were compared, as well as diagnostic and therapeutic aspects; we concluded that coexistence of both entities is infrequent but may occur due to increased presentation of the diseases. | 2003 | 12754952 |
| predicting gene expression levels from codon biases in alpha-proteobacterial genomes. | predicted highly expressed (phx) genes in five currently available high g+c complete alpha-proteobacterial genomes are analyzed. these include: the nitrogen-fixing plant symbionts sinorhizobium meliloti (sinme) and mesorhizobium loti (meslo), the nonpathogenic aquatic bacterium caulobacter crescentus (caucr), the plant pathogen agrobacterium tumefaciens (agrtu), and the mammalian pathogen brucella melitensis (brume). three of these genomes, sinme, agrtu, and brume, contain multiple chromosomes o ... | 2003 | 12775761 |
| brucellar spinal epidural abscess. | 2003 | 12776958 | |
| evaluation of a modified rose bengal test and an indirect enzyme-linked immunosorbent assay for the diagnosis of brucella melitensis infection in sheep. | a modified rose bengal test (mrb) and an indirect elisa (ielisa) with protein g as the conjugate, were evaluated for the diagnosis of brucella melitensis infection in unvaccinated sheep with a known bacteriological status, and their diagnostic efficacy was compared with that of the standard rose bengal (rb) and complement fixation (cf) tests used in the current eradication campaign in eu countries. all tests showed 100% specificity when testing the sera from 212 brucella-free sheep. when testing ... | 2003 | 12791239 |
| persisting fever in a patient with brucella endocarditis: occult splenic abscess. | brucella endocarditis, despite its high mortality rate with combined medical and surgical treatment, has a low occurrence rate in cases of brucellosis and has been endemic in regions surrounding turkey. rarely, patients with infective endocarditis with common microorganisms develop a splenic abscess. a patient is reported on with brucella endocarditis and persistent fever. an occult splenic abscess was found. this is the second reported case in the literature of brucella endocarditis with spleni ... | 2003 | 12807868 |
| epitope mapping of the brucella melitensis bp26 immunogenic protein: usefulness for diagnosis of sheep brucellosis. | sequencing of bp26, the gene encoding the brucella sp. immunogenic bp26 periplasmic protein, was performed in the reference strains of brucella abortus, b. suis, and b. ovis. the three bp26 sequences were almost identical to that published for b. melitensis 16m bp26, and only minor nucleotide substitutions, without modifying the amino acid sequence, were observed between species. the bp26 genes of the seven b. abortus biovar reference strains and b. abortus s19 and rb51 vaccine strains were also ... | 2003 | 12853399 |
| expression cloning and biochemical characterization of a rhizobium leguminosarum lipid a 1-phosphatase. | lipid a of rhizobium leguminosarum, a nitrogen-fixing plant endosymbiont, displays several significant structural differences when compared with escherichia coli. an especially striking feature of r. leguminosarum lipid a is that it lacks both the 1- and 4'-phosphate groups. distinct lipid a phosphatases that attack either the 1 or the 4' positions have previously been identified in extracts of r. leguminosarum and rhizobium etli but not sinorhizobium meliloti or e. coli. here we describe the id ... | 2003 | 12869541 |
| characterization of new members of the group 3 outer membrane protein family of brucella spp. | impairment of the omp25 gene in brucella spp. leads to attenuated strains and confers protection to the host. omp25 and omp31, whose functions remain unknown, were the first characterized members of group 3 outer membrane proteins (omps) (25 to 34 kda). recently, genomic and proteomic approaches identified five new putative members of this family, some of which are produced in b. melitensis or b. abortus. in the present study, using protein microsequencing, we identified new members of group 3 o ... | 2003 | 12874309 |
| biotypes and antimicrobial susceptibilities of brucella isolates. | 41 brucella strains isolated from blood and cerebrospinal fluid cultures were identified to species level and biotypes detected. all of the isolates were brucella melitensis: 2 strains of b. melitensis biotype-1 and 39 strains of b. melitensis biotype-3. in vitro activities of these strains were detected by the e test method. according to the 90% minimal inhibitory concentration (mic90) values, the most active agent was doxycycline (mic90 0.064 microg/ml), followed by ciprofloxacin (mic90 0.25 m ... | 2003 | 12875523 |
| spontaneous bacterial peritonitis caused by brucella melitensis. | this report presents a case of blood and ascitic fluid culture-proven spontaneous bacterial peritonitis caused by brucella melitensis in a 67-y-old male with congestive heart failure. ten cases of reported previously brucella peritonitis are reviewed, 3 of which were ascitic fluid culture negative. | 2003 | 12875525 |
| molecular host-pathogen interaction in brucellosis: current understanding and future approaches to vaccine development for mice and humans. | brucellosis caused by brucella spp. is a major zoonotic disease. control of brucellosis in agricultural animals is a prerequisite for the prevention of this disease in human beings. recently, brucella melitensis was declared by the centers for disease control and prevention to be one of three major bioterrorist agents due to the expense required for the treatment of human brucellosis patients. also, the economic agricultural loss due to bovine brucellosis emphasizes the financial impact of bruce ... | 2003 | 12525425 |
| purification and characterization of an immunogenic aminopeptidase of brucella melitensis. | an immunogenic aminopeptidase was purified from brucella melitensis strain vtrm1. the purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. this procedure resulted in a yield of 29% and a 144-fold increase in specific activity. the aminopeptidase appeared to be a monomeric enzyme with a molecular mass of 96 kda and an isoelectric point of 4.8. its activity was optimal at ph 7.0 at 40 degrees c. the enzyme was strongly inhibited by edta, 1,10-phenathr ... | 2003 | 12933870 |
| epidemiology of ovine brucellosis in awassi sheep in northern jordan. | we used a combined cross-sectional and longitudinal design to estimate seroprevalence of brucella antibodies in awassi sheep and the incidence of abortion due to brucellosis during one lambing season, and to test risk factors. the brucella organisms isolated from aborted fetuses and vaginal swabs were characterized as brucella melitensis biotype 3. seventy awassi sheep flocks were selected randomly from northern jordan. sixty two of the 70 flocks were used in the cross-sectional study and 8 floc ... | 2003 | 12941554 |
| brucellosis outbreak due to unpasteurized raw goat cheese in andalucia (spain), january - march 2002. | eleven brucellosis cases were identified in three municipalities of cordoba (andalucia, spain). a case-control study was conducted, selecting three cases per control. persons having eaten unpasteurized raw goat cheese produced in a farmhouse located in the epidemic territory, were at higher risk for presenting brucellosis (or=21.6, ic95%=1.6-639.8). brucella melitensis serovar 3 was identified in clinical specimens and in goat tissue and milk samples from the herd's farmhouse. preventive measure ... | 2003 | 12941982 |
| neurobrucellosis presenting as leukoencephalopathy: the role of cytotoxic t lymphocytes. | a 65-year-old man developed a leukoencephalopathy associated with neurobrucellosis. the disease followed a 15-month progressive course with neurologic symptoms, and magnetic resonance imaging revealed bilateral symmetrical t2 signal hyperintensities in the white matter. biopsy of the cerebral cortex and white matter was significant for nongranulomatous meningoencephalitis with reactive microgliosis and astrogliosis. the inflammatory infiltrate was predominantly composed of t lymphocytes, includi ... | 2003 | 12946213 |
| shell vial culture as a tool for isolation of brucella melitensis in chronic hepatic abscess. | a 50-year-old man was admitted with a liver abscess and positive serology for brucella spp. liver pus and blood cultures remained sterile on conventional culture. inoculation of liver pus onto eukaryotic cells by the centrifugation-shell vial technique yielded brucella melitensis, identified by 16s rrna gene amplification and sequencing. | 2003 | 12958294 |
| fluorescence polarization assay for diagnosis of human brucellosis. | fluorescence polarization immunoassay (fpa) uses molecular rotational properties to measure antibody binding to antigen directly. the potential use of this method was assessed in comparison to a competitive enzyme immunoassay (celisa) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the fpa was 97.9 % using a cut-off value of 72 mp. sera from bruce ... | 2003 | 12972582 |
| brucellosis and the respiratory system. | brucellosis is a zoonotic disease that remains endemic worldwide. its clinical manifestations and focal complications are often troublesome in making a diagnosis. involvement of the respiratory system in brucellosis is an acknowledged but rare event that is only occasionally described in literature. we describe 37 cases of respiratory involvement during the course of brucellosis that presented as pneumonia, bronchopneumonia, pleural effusion with a predominance of monocytic or lymphocytic infilt ... | 2003 | 13130417 |
| statistical procedures for calculating the residual virulence of brucella abortus strain 19 (s19) and brucella melitensis strain rev 1 vaccines in mice: theoretical basis and practical applications. | regular control of the biological quality of live brucella abortus strain 19 (s19) and b. melitensis strain rev 1 vaccines is essential for the successful management of ruminant brucellosis in affected countries. the reference procedures recommended by the oie (world organisation for animal health) and the european pharmacopoeia include the determination of residual virulence, expressed as the recovery time 50 (rt50), of the tested (problem) vaccine in a reference mouse model compared with the r ... | 2003 | 15005562 |
| identification of species of brucella using fourier transform infrared spectroscopy. | fourier transform infrared spectroscopy (ftir) is a technique that has been used over the years in chemical analysis for the identification of substances and is one that may be applied to the characterisation of microorganisms. the marked tendency of brucella towards variation in the smooth rough phase, together with the laboriousness and risk involved in the methods used in their identification, make their classification difficult. we studied the type strains of the different species and biovar ... | 2003 | 14500003 |
| neurobrucellosis and a demonstration of its involvement in spinal roots via magnetic resonance imaging. | to present a neurobrucellosis case with spinal root involvement by means of magnetic resonance imaging (mri). | 2003 | 14504617 |
| anatomy of an exposure: a hospital lab's recovery of brucella melitensis. | 2003 | 14524145 | |
| cloning and expression of bartonella henselae sucb gene encoding an immunogenic dihydrolipoamide succinyltransferase homologous protein. | immunoscreening of a zap genomic library of bartonella henselae strain houston-1 expressed in escherichia coli resulted in the isolation of a clone containing 3.5 kb bamhi genomic dna fragment. this 3.5 kb dna fragment was found to contain a sequence of a gene encoding a protein with significant homology to the dihydrolipoamide succinyltransferase of brucella melitensis (sucb). subsequent cloning and dna sequence analysis revealed that the deduced amino acid sequence from the cloned gene showed ... | 2003 | 14524617 |
| [acute calculous cholecystitis associated with brucella melitensis]. | 2003 | 14525714 | |
| molecular characterization of brucella abortus chromosome ii recombination. | large-scale genomic rearrangements including inversions, deletions, and duplications are significant in bacterial evolution. the recently completed brucella melitensis 16m and brucella suis 1330 genomes have facilitated the investigation of such events in the brucella spp. suppressive subtractive hybridization (ssh) was employed in identifying genomic differences between b. melitensis 16m and brucella abortus 2308. analysis of 45 ssh clones revealed several deletions on chromosomes of b. abortus ... | 2003 | 14526025 |
| acute brucellosis presenting with erythema nodosum. | brucellosis is a common world-wide zoonotic disease. cutaneous manifestations are not specific and affect 1-14% of patients with brucellosis. we describe two cases of young males presenting with skin lesions of erythema nodosum on the anterior surface of the legs. histopathology of skin biopsy revealed septal panniculitis, but the positive cultures of blood or bone marrow for brucella melitensis established the diagnosis of brucellosis. | 2003 | 14561053 |
| human mammary abscess caused by brucella melitensis: a case report. | 2003 | 14563232 | |
| activation and proliferation of t lymphocyte subpopulations in patients with brucellosis. | t-cell proliferation is a standard method to evaluate cellular immune responses against intracellular infectious agents. the present study was undertaken to look for expression of an early activation marker (cd69) and proliferation using a nonradioactive method to evaluate cellular immune response against a salt-extractable antigen from brucella melitensis 16m (rcm-bm) in patients suffering from brucellosis. | 2003 | 14567397 |
| [evaluation of 2 hemoculture media for the isolation of brucella spp.]]. | the diagnostic efficiency of two hemoculture media for the detection of different species of brucella strains was evaluated. strains of brucella melitensis, brucella suis, brucella abortus, brucella ovis, and brucella abortus s19 were used. each strain was diluted in phosphate buffer saline (pbs) to obtain a concentration of 10(5) colony forming units/ml (cfu/ml). blood from goats, pigs, cattle, and sheep was mixed with the bacterial suspension to obtain a final concentration minor or equal to 1 ... | 2003 | 14587372 |
| ovarian abscess due to brucella melitensis. | the case of a 25-y-old woman with brucellar ovarian abscess is reported. cultures of blood, ascites and a pus specimen yielded brucella melitensis. the possibility of ovarian abscess being caused by brucella melitensis should be considered in countries where the infection is endemic. | 2003 | 14606619 |
| spontaneous bacterial peritonitis due to brucella infection. | brucella infection is a systemic disease, but the microorganism rarely causes infections in the gastrointestinal system such as hepatitis, cholecystitis, colitis and pancreatitis. spontaneous bacterial peritonitis due to brucella is extremely rare. herein, we report a case of cirrhosis complicated with nongranulomatous hepatitis and peritonitis, both due to brucella. a 63 year-old man with diabetes mellitus was admitted to hospital with complaints of weakness, backache, abdominal pain and abdomi ... | 2003 | 14614644 |
| brucella spondylitis and sacroiliitis in the general population in mumbai. | 1) to identify patients of brucella spondylitis and sacroiliitis amongst patients of 'infective spondylitis' and 'sacroiliitis', 2) to study clinical and radiographic features and outcome of brucella spondylitis. | 2003 | 14621032 |
| attenuated signature-tagged mutagenesis mutants of brucella melitensis identified during the acute phase of infection in mice. | for this study, we screened 1,152 signature-tagged mutagenesis mutants of brucella melitensis 16m in a mouse model of infection and found 36 of them to be attenuated in vivo. molecular characterization of transposon insertion sites showed that for four mutants, the affected genes were only present in rhizobiaceae. another mutant contained a disruption in a gene homologous to mosa, which is involved in rhizopine biosynthesis in some strains of rhizobium, suggesting that this sugar may be involved ... | 2003 | 14638795 |
| the effects of streptozotocin-induced diabetes on brucellosis of rats. | it is believed that an infection is more common and runs a more protracted course in people with diabetes. in clinical practice, it is important to be aware of these associations, as the prognosis is often dependent upon prompt recognition and appropriate treatment. to show the course of brucellosis in the diabetic state, a model of brucella melitensis infection was used in the setting of streptozotocin-induced diabetes in rat. b. melitensis infection proceeded more severely in diabetic rats and ... | 2003 | 14642313 |
| neglected case of osteoarticular brucella infection of the knee. | a 49-year-old farmer had a history of recurrent knee effusion for 20 years. he did not report undergoing any diagnostic or therapeutic procedures apart from repeated aspirations of the joint fluid. after the isolation of brucella melitensis from the joint fluid, computed tomography-guided bone biopsy was performed and histopathologic examination of the biopsy sample confirmed the diagnosis of chronic brucella osteomyelitis. arthroscopic synovectomy combined with antimicrobial therapy with doxycy ... | 2003 | 14652892 |
| pathways for phosphatidylcholine biosynthesis in bacteria. | phosphatidylcholine (pc) is the major membrane-forming phospholipid in eukaryotes with important structural and signalling functions. although many prokaryotes lack pc, it can be found in significant amounts in membranes of rather diverse bacteria. two pathways for pc biosynthesis are known in bacteria, the methylation pathway and the phosphatidylcholine synthase (pcs) pathway. in the methylation pathway, phosphatidylethanolamine is methylated three times to yield pc, in reactions catalysed by o ... | 2003 | 14663079 |
| the effect of levamisole combined with the classical treatment in chronic brucellosis. | levamisole is an immunopotenciator drug which is used as an antihelmintic drug as well as very effective remedy on cellular immunity compared with humoral immunity. a total 71 patients (37 men, 34 women) who referred to our department between march 1997 and december 2001, with a history of the disease for about 1 year, were diagnosed as having chronic brucellosis through those tests brucella serum agglutination test (sat), sat with coombs and sat with 2-mercaptoethanol. the patients were randoml ... | 2003 | 14690014 |
| guillain-barré syndrome associated with acute neurobrucellosis. | we report the case of a 14-year-old girl with guillain-barré syndrome associated with brucellosis due to brucella melitensis. the diagnosis was established by the isolation of b. melitensis from her blood and by the determination of high levels of brucella aglutinins in her sera and cerebrospinal fluid. a combination of rifampin, co-trimoxazole and physical therapy resulted in complete healing within 30 days. antibrucellar treatment continued for 12 weeks. this case report suggests that brucello ... | 2003 | 14712899 |
| brucellosis as a cause of prolonged fever in an era of pasteurized milk. | 2003 | 15260406 | |
| repeatability and pattern recognition of bacterial fatty acid profiles generated by direct mass spectrometric analysis of in situ thermal hydrolysis/methylation of whole cells. | direct ci mass spectrometry profiling of fatty acid methyl esters (fames) from in situ thermal hydrolysis/methylation (thm) of whole bacterial cells with tetramethylammonium hydroxide (tmah) has been demonstrated as a potential method for real time and fieldable detection/identification of microorganisms. bacillus anthracis (ames), yersinia pestis (nair. kenya), vibrio cholerae (e1 tor), brucella melitensis (abortus wild) and francisella tularensis (lvs vaccine) were profiled by this method duri ... | 2003 | 18968943 |
| kinetics of the antibody response in ewes experimentally infected with brucella melitensis biovar 3. | the authors evaluated the kinetics of antibody response in 46 ewes coming from officially brucellosis free flocks that were experimentally infected with brucella melitensis biovar 3, and monitored through three subsequent reproductive cycles. in this study, results of rose bengal test (rbt) and complement fixation test (cft) were considered. test results of 2nd and 3rd reproductive cycle show a peak in the antibody production at parturition, followed by a drop in the following months. the peak a ... | 2004 | 20437389 |
| comparative whole-genome hybridization reveals genomic islands in brucella species. | brucella species are responsible for brucellosis, a worldwide zoonotic disease causing abortion in domestic animals and malta fever in humans. based on host preference, the genus is divided into six species. brucella abortus, b. melitensis, and b. suis are pathogenic to humans, whereas b. ovis and b. neotomae are nonpathogenic to humans and b. canis human infections are rare. limited genome diversity exists among brucella species. comparison of brucella species whole genomes is, therefore, likel ... | 2004 | 15262941 |
| case report: brucellosis: a re-emerging disease in thailand. | brucellosis is a zoonotic disease prevalent in many countries, but it has been reported only once in thailand, 36 years ago. we describe here two consecutive cases of brucellosis in bangkok, thailand. both cases presented with prolonged fever and weight loss. blood cultures taken from 2 patients yielded brucella melitensis. the slide agglutination test of blood samples were also positive, with a titer of 1:64 for antibodies to brucella. the first patient responded to a combination of doxycycline ... | 2004 | 15272752 |
| [brucella myopericarditis]. | 2004 | 15274859 | |
| role of catalase in the virulence of brucella melitensis in pregnant goats. | an isogenic kate mutant derived from virulent brucella melitensis 16m displays hypersensitivity to hydrogen peroxide in disk sensitivity assays but retains the capacity to colonize pregnant goats and induce abortion. these experimental findings indicate that although the sole periplasmic catalase of brucella melitensis functions as an antioxidant, this enzyme does not play a critical role in virulence in the natural host. | 2004 | 15288933 |
| immunogenicity of recombinant omp31 from brucella melitensis in rams and serum bactericidal activity against b. ovis. | detergent-extracted recombinant omp31 (romp31 extract) from brucella melitensis produced in escherichia coli was previously identified as a protective immunogen against b. ovis in mice. in this study, we evaluated the immunogenicity of romp31extract in rams. this immunogen was emulsified in an oil adjuvant and administered three times with 4 and 8 weeks intervals. antibody response was measured in serum by whole b. ovis elisa. specific antibodies to purified romp31 (pet-omp31) were detected by w ... | 2004 | 15327795 |
| enhanced efficacy of recombinant brucella abortus rb51 vaccines against b. melitensis infection in mice. | brucella abortus strain rb51 is an attenuated rough strain, currently being used as the official live vaccine for bovine brucellosis in the usa and several other countries. in strain rb51, the wboa gene, encoding a glycosyltransferase required for the o-side chain synthesis, is disrupted by an is711 element. recently, we have demonstrated that strain rb51wboa, rb51 complemented with a functional wboa gene, remains rough but expresses low quantities of o-side chain in the cytoplasm. mice vaccinat ... | 2004 | 15327798 |
| antibody reactivity to omp31 from brucella melitensis in human and animal infections by smooth and rough brucellae. | group 3 of outer membrane proteins (omps) of brucella includes omp25 and omp31, which share 34% identity. omp25 is highly conserved in brucella species, and omp31 is present in all brucella species, except brucella abortus. antibodies to brucella melitensis omp31 have been sought only in infected sheep, and western blotting of sera from infected sheep did not reveal anti-omp31 reactivity. we obtained recombinant purified omp31 (b. melitensis) and tested its recognition by sera from humans and an ... | 2004 | 14715555 |
| fever without apparent source on clinical examination. | episodes of acute illness associated with fever are common in children. less common but also diagnostically challenging are episodes of prolonged fever in children. this article reports recent literature that has focused on the epidemiology, pathophysiology, clinical and laboratory evaluation, and treatment of both types of episodes. | 2004 | 14758122 |
| bartholin's gland abscess caused by brucella melitensis. | we report herein a case of bartholin's gland abscess caused by brucella melitensis. clinical microbiology laboratory workers in areas where this disease is endemic should be familiar with the bacteriological features of this organism and consider the possibility of a brucellar etiology in a broad range of clinical settings. | 2004 | 14766890 |
| generation of the brucella melitensis orfeome version 1.1. | the bacteria of the brucella genus are responsible for a worldwide zoonosis called brucellosis. they belong to the alpha-proteobacteria group, as many other bacteria that live in close association with a eukaryotic host. importantly, the brucellae are mainly intracellular pathogens, and the molecular mechanisms of their virulence are still poorly understood. using the complete genome sequence of brucella melitensis, we generated a database of protein-coding open reading frames (orfs) and constru ... | 2004 | 15489343 |
| a case of intracranial abscess due to brucella melitensis. | 2004 | 15494262 | |
| laboratory-acquired brucellosis. | we report two laboratory-acquired brucella melitensis infections that were shown to be epidemiologically related. blood culture isolates were initially misidentified because of variable gram stain results, which led to misdiagnoses and subsequent laboratory exposures. notifying laboratory personnel who unknowingly processed cultures from brucellosis patients is an important preventive measure. | 2004 | 15504276 |
| modeling of seb-induced host gene expression to correlate in vitro to in vivo responses. | detection of exposure to biological threat agents has relied on ever more sensitive methods for pathogen identification, but that usually requires pathogen proliferation to dangerous, near untreatable levels. recent events have demonstrated that assessing exposure to a biological threat agent well in advance of onset of illness or at various stages post-exposure is invaluable among the diagnostic options. there is an urgent need for better diagnostic tools that will be sensitive, rapid, and unam ... | 2004 | 15522586 |
| brucella endocarditis complicated with a mycotic aneurysm of the superior mesenteric artery: a case report. | brucella endocarditis, although a rare complication of brucellosis, is the main cause of death attributable to this disease. mycotic aneurysm is an important extracardiac complication of infective endocarditis. mycotic aneurysm of the superior mesenteric artery is extremely rare. in the present case, we report a patient with brucella melitensis endocarditis complicated with a superior mesenteric artery aneurysm. to our knowledge, this is the first such reported case. | 2004 | 14975571 |
| brucella pathogenesis, genes identified from random large-scale screens. | pathogenicity islands, specialized secretion systems, virulence plasmids, fimbriae, pili, adhesins, and toxins are all classical bacterial virulence factors. however, many of these factors, though widespread among bacterial pathogens, are not necessarily found among bacteria that colonize eukaryotic cells in a pathogenic/symbiotic relationship. bacteria that form these relationships have developed other strategies to infect and grow in their hosts. this is particularly true for brucella and othe ... | 2004 | 14979322 |
| use of an immunobinding test on nitrocellulose paper to diagnose caprine brucellosis. | 2004 | 14989360 | |
| protection against brucellosis in goats, five years after vaccination with reduced-dose brucella melitensis rev 1 vaccine. | the protection conferred by the reduced-dose rev 1 brucella melitensis vaccine in goats that had been immunized 5 years previously was evaluated. sixteen goats vaccinated 5 years before with rev 1 (1 x 10(5) cfu) and 5 non-vaccinated goats were challenged with b. melitensis 16m (4 x 10(5) cfu) using the conjunctival route. after giving birth or aborting, the goats were sacrificed and tissue samples were taken for bacteriological study. the challenge strain was recovered in 12%, of the animals fr ... | 2004 | 14998310 |
| real-time multiplex pcr assay for detection of brucella spp., b. abortus, and b. melitensis. | the identification of brucella can be a time-consuming and labor-intensive process that places personnel at risk for laboratory-acquired infection. here, we describe a real-time pcr assay for confirmation of presumptive brucella isolates. the assay was designed in a multiplex format that will allow the rapid identification of brucella spp., b. abortus, and b. melitensis in a single test. | 2004 | 15004098 |
| different responses of macrophages to smooth and rough brucella spp.: relationship to virulence. | by comparing smooth wild-type brucella strains to their rough mutants, we show that the lipopolysaccharide (lps) o side chain of pathogenic brucella has a dramatic impact on macrophage activation. it favors the development of virulent brucella by preventing the synthesis of immune mediators, important for host defense. we conclude that this o chain property is firmly linked to brucella virulence. | 2004 | 15039375 |
| adherence of brucella to human epithelial cells and macrophages is mediated by sialic acid residues. | the basis for the interaction of brucella species with the surface of epithelial cells before migration in the host within polymorphonuclear leucocytes is largely unknown. here, we studied the ability of brucella abortus and brucella melitensis to adhere to cultured epithelial (hela and hep-2) cells and thp-1-derived macrophages, and to bind extracellular matrix proteins (ecm). the brucellae adhered to epithelial cells forming localized bacterial microcolonies on the cell surface, and this proce ... | 2004 | 15056214 |
| in vitro antimicrobial susceptibility of brucella species. | mic50 and mic90 values of doxycycline, rifampicin, ciprofloxacin, trimethoprim-sulphamethoxazole and ceftriaxone for 42 blood isolates of brucella species were determined using the etest. thirty-seven isolates were identified as b. melitensis and five as b. abortus. doxycycline had the lowest and rifampicin the highest mic50 values. four strains were non-susceptible to rifampicin, and one strain was resistant to trimethoprim-sulphamethoxazole. there is no significantly important resistance probl ... | 2004 | 15081093 |
| a case of mitral stenosis complicated with seronegative brucella endocarditis. | brucellosis is a multisystemic disease. the most common cause of death from the disease is endocarditis. the aortic valve is most commonly affected. the disease rarely involves the mitral valve. a 30 year-old woman presented with complaints of chills and fever up to 38 degrees c at night, fatigue, palpitations, and dyspnea for the previous 3 weeks. cardiac auscultation revealed a diastolic murmur in the mitral area. her temperature was 38.3 degrees c. on echocardiographic examination, the mitral ... | 2004 | 15090714 |
| an in vitro study on antimicrobial activity of propolis from mugla province of turkey. | antibacterial and antifungal activities of acetone and dimethyl sulfoxide (dmso) extracts of 45 different propolis samples from the mugla province of turkey were investigated. antimicrobial activity of propolis varied depending on propolis sample, dosage of propolis, and the extraction solvents for all test microorganisms. antimicrobial activity of all propolis samples increased with increasing dosage without reaching a plateau at the highest dosage tested. except for brucella melitensis, the dm ... | 2004 | 15117559 |