Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| characterization of the mrgrs locus of the opportunistic pathogen burkholderia pseudomallei: temperature regulates the expression of a two-component signal transduction system. | burkholderia pseudomallei is a saprophyte in tropical environments and an opportunistic human pathogen. this versatility requires a sensing mechanism that allows the bacterium to respond rapidly to altered environmental conditions. we characterized a two-component signal transduction locus from b. pseudomallei 204, mrgr and mrgs, encoding products with extensive homology with response regulators and histidine protein kinases of escherichia coli, bordetella pertussis, and vibrio cholerae. | 2006 | 16893462 |
| imported melioidosis--south florida, 2005. | in 2005, two cases of melioidosis (one in august, one in october) were reported to the florida department of health, the first cases since reporting the disease became mandatory in florida in 2003. in one case, burkholderia pseudomallei was not recognized as the bacterium that causes the disease melioidosis, which led to a delay in reporting the case to the local health department. in both cases, delayed recognition and unsafe laboratory practices resulted in laboratory workers being exposed to ... | 2006 | 16915220 |
| the innate interferon gamma response of balb/c and c57bl/6 mice to in vitro burkholderia pseudomallei infection. | burkholderia pseudomallei is the causative agent for melioidosis. for many bacterial infections, cytokine dysregulation is one of the contributing factors to the severe clinical outcomes in the susceptible hosts. the c57bl/6 and balb/c mice have been established as a differential model of susceptibility in murine melioidosis. in this study, we compared the innate ifn-gamma response to b. pseudomallei between the c57bl/6 and balb/c splenocytes and characterized the hyperproduction of ifn-gamma in ... | 2006 | 16919160 |
| in silico analysis of burkholderia pseudomallei genome sequence for potential drug targets. | recent advances in dna sequencing technology have enabled elucidation of whole genome information from a plethora of organisms. in parallel with this technology, various bioinformatics tools have driven the comparative analysis of the genome sequences between species and within isolates. while drawing meaningful conclusions from a large amount of raw material, computer-aided identification of suitable targets for further experimental analysis and characterization, has also led to the prediction ... | 2006 | 16922696 |
| temperature-regulated microcolony formation by burkholderia pseudomallei requires pila and enhances association with cultured human cells. | burkholderia pseudomallei is the causative agent of melioidosis, a potentially fatal disease that is endemic to northern australia and southeast asia and is acquired from soil or water. adherence of b. pseudomallei 08 to cultured cells increases dramatically following prior growth at 30 degrees c or less compared to that following prior growth at 37 degrees c. here, we show that this occurs almost entirely as the result of microcolony formation (bacterium-bacterium interactions) following growth ... | 2006 | 16926432 |
| development of mouse hybridomas for production of monoclonal antibodies specific to burkholderia mallei and burkholderia pseudomallei. | burkholderia mallei and b. pseudomallei are designated category b biothreat agents on the "select agents" list established by the nih and cdc. development of monoclonal antibodies (mabs) that could effectively differentiate these two closely related species of bacteria and other non-pathogenic burkholderia bacteria is urgently needed. splenocytes from mice immunized with various antigen preparations from either b. mallei (american type culture collection [atcc] 23344) or b. pseudomallei (atcc 23 ... | 2006 | 16934015 |
| pyogenic liver abscess caused by burkholderia pseudomallei in taiwan. | pyogenic liver abscess in taiwan is a well-known disease entity, commonly associated with a single pathogen, klebsiella pneumoniae. melioidosis is an endemic disease in taiwan that can manifest as multiple abscesses in sites including the liver. we report three cases of liver abscesses caused by burkholderia pseudomallei. the first patient was a 54-year-old diabetic woman, who presented with liver abscess and a left subphrenic abscess resulting from a ruptured splenic abscess, co-infected with k ... | 2006 | 16935773 |
| melioidosis. | melioidosis is increasingly recognized around the world. despite several decades of clinical research, the mortality rate for melioidosis remains high. this review focuses on studies that relate to patient management, including risk factors, diagnosis, treatment and prediction of the outcome. a brief summary of studies relating to genomics and genotyping, immunology and pathogenesis is provided. | 2006 | 16940864 |
| a man with a saccular aneurysm of the left common iliac artery. | 2006 | 16941374 | |
| expression, purification, crystallization and preliminary crystallographic analysis of bipd, a component of the burkholderia pseudomallei type iii secretion system. | a construct consisting of residues 10-310 of bipd, a component of the burkholderia pseudomallei type iii secretion system (t3ss), has been overexpressed as a gst fusion, cleaved from the gst tag and purified. crystals were grown of native and selenomethionine-labelled bipd. the crystals grow in two different polymorphs from the same condition. the first polymorph belongs to space group c222, with unit-cell parameters a = 103.98, b = 122.79, c = 49.17 a, a calculated matthews coefficient of 2.4 a ... | 2006 | 16946464 |
| high resolution structure of bipd: an invasion protein associated with the type iii secretion system of burkholderia pseudomallei. | burkoldheria pseudomallei is a gram-negative bacterium that possesses a protein secretion system similar to those found in salmonella and shigella. recent work has indicated that the protein encoded by the bipd gene of b. pseudomallei is an important secreted virulence factor. bipd is similar in sequence to ipad from shigella and sipd from salmonella and is therefore likely to be a translocator protein in the type-iii secretion system of b. pseudomallei. the crystal structure of bipd has been so ... | 2006 | 16950399 |
| antibiotic susceptibility of 50 clinical isolates of burkholderia pseudomallei from singapore. | 2006 | 16951412 | |
| burkholderia oklahomensis sp. nov., a burkholderia pseudomallei-like species formerly known as the oklahoma strain of pseudomonas pseudomallei. | c6786, the clinical isolate of the 'oklahoma' strain of pseudomonas (now burkholderia) pseudomallei, was originally isolated in 1973 from a wound infection resulting from a farming accident in oklahoma, usa. environmental isolates c7532 and c7533 from the oklahoma accident site were found to match c6786. these three isolates and a clinical isolate originally identified as b. pseudomallei that was recovered from a person in georgia, usa, involved in an automobile accident were characterized by bi ... | 2006 | 16957116 |
| environmental factors that affect the survival and persistence of burkholderia pseudomallei. | 2006 | 16980433 | |
| melioidosis: an imported case from madagascar. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia but is a rare pathogen in other parts of the world. no human case of melioidosis has been reported in madagascar until now. we describe a case of pulmonary melioidosis probably acquired in madagascar. | 2006 | 16987131 |
| a live experimental vaccine against burkholderia pseudomallei elicits cd4+ t cell-mediated immunity, priming t cells specific for 2 type iii secretion system proteins. | burkholderia pseudomallei is the etiological agent of melioidosis, a serious human disease for which no vaccine is available. immunization of susceptible balb/c mice with the live attenuated mutant b. pseudomallei ilvi (referred to as "2d2") generated significant, although incomplete, immunity. splenic b. pseudomallei-specific t cells, detected in immunized mice, proliferated and produced interferon-gamma in vitro in response to dead bacteria. assessment of t cell antigen specificity indicated t ... | 2006 | 17041850 |
| activity of tigecycline in the treatment of acute burkholderia pseudomallei infection in a murine model. | burkholderia pseudomallei is the causative agent of melioidosis. standard therapy includes ceftazidime alone or in combination with co-trimoxazole. tigecycline, a novel agent, has displayed activity against b. pseudomallei. we evaluated the in vivo efficacy of tigecycline using a murine model of melioidosis. mice were infected with either a high or low virulence b. pseudomallei isolate followed by administration of antibiotics alone or in combination (tigecycline, ceftazidime, tigecycline plus c ... | 2006 | 17046208 |
| pneumonia and septicemia caused by burkholderia thailandensis in the united states. | burkholderia thailandensis is closely related to burkholderia pseudomallei, the causative agent of melioidosis. it is generally considered avirulent and previously has been reported to occur only in southeast asia. we report the first case of pneumonia and septicemia caused by b. thailandensis in the united states. | 2006 | 17050819 |
| cutaneous melioidosis. | melioidosis is an infection caused by the gram-negative bacterium burkholderia pseudomallei. this disease is endemic in southeast asia and north australia with sporadic occurrence in temperate countries, mostly imported from travellers. any organ can be involved in melioidosis whereby burkholderia pseudomallei causes an acute inflammatory reaction with rapid development of small abcesses which tend to coalesce to form larger abscesses. cutaneous manifestations vary greatly. we report a man with ... | 2006 | 17062053 |
| genome-wide expression analysis of burkholderia pseudomallei infection in a hamster model of acute melioidosis. | burkholderia pseudomallei is the causative agent of melioidosis and represents a potential bioterrorism threat. in the current studies we have examined gene expression in b. pseudomallei in an animal model of acute melioidosis using whole-genome microarrays. gene expression profiles were generated by comparing transcriptional levels of b. pseudomallei-expressed genes in infected hamster organs including liver, lung, and spleen following intraperitoneal and intranasal routes of infection to those ... | 2006 | 16988221 |
| global map of growth-regulated gene expression in burkholderia pseudomallei, the causative agent of melioidosis. | many microbial pathogens express specific virulence traits at distinct growth phases. to understand the molecular pathways linking bacterial growth to pathogenicity, we have characterized the growth transcriptome of burkholderia pseudomallei, the causative agent of melioidosis. using a fine-scale sampling approach, we found approximately 17% of all b. pseudomallei genes displaying regulated expression during growth in rich medium, occurring as broad waves of functionally coherent gene expression ... | 2006 | 16997946 |
| role of inducible nitric oxide synthase and nadph oxidase in early control of burkholderia pseudomallei infection in mice. | infection with the soil bacterium burkholderia pseudomallei can result in a variety of clinical outcomes, including asymptomatic infection. the initial immune defense mechanisms which might contribute to the various outcomes after environmental contact with b. pseudomallei are largely unknown. we have previously shown that relatively resistant c57bl/6 mice can restrict bacterial b. pseudomallei growth more efficiently within 1 day after infection than highly susceptible balb/c mice. by using thi ... | 2006 | 17000727 |
| pharmacokinetic and pharmacodynamic assessment of co-amoxiclav in the treatment of melioidosis. | we conducted a prospective pharmacokinetic study of oral co-amoxiclav in patients with melioidosis to determine the optimal dosage and dosing interval in this potentially fatal infection. | 2006 | 17003061 |
| method for regulated expression of single-copy efflux pump genes in a surrogate pseudomonas aeruginosa strain: identification of the bpeef-oprc chloramphenicol and trimethoprim efflux pump of burkholderia pseudomallei 1026b. | construction and integration of recombinant mini-tn7 expression vectors into the chromosome of a surrogate, efflux-sensitized, and biosafe pseudomonas aeruginosa host was validated as a generally applicable method for studies of uncharacterized bacterial efflux pumps. using this method, the burkholderia pseudomallei bpeef-oprc operon was shown to encode a chloramphenicol and trimethoprim efflux pump. | 2006 | 17005832 |
| melioidosis in an italian tourist injured by the tsunami in thailand. | 2006 | 17024805 | |
| pcr-based identification of burkholderia pseudomallei. | dna amplification techniques are being used increasingly in clinical laboratories to confirm the identity of medically important bacteria. a pcr-based identification method has been in use in our centre for 10 years for burkholderia pseudomallei and was used to confirm the identity of bacteria isolated from cases of melioidosis in ceará since 2003. this particular method has been used as a reference standard for less discriminatory methods. in this study we evaluated three pcr-based methods of b ... | 2006 | 17086309 |
| in vitro motility of a population of clinical burkholderia pseudomallei isolates. | melioidosis, a serious infection caused by burkholderia pseudomallei, is a leading cause of community-acquired sepsis in northeast thailand, and the commonest cause of death from community-acquired pneumonia in the top end of northern australia. the causative organism is a gram-negative, motile bacillus that is a facultative intracellular pathogen. b. pseudomallei flagella have been proposed as a possible vaccine candidate and putative virulence determinant. flagella expression was highly conser ... | 2006 | 17100392 |
| short report: melioidosis in myanmar: forgotten but not gone? | a serologic survey of adults resident in myanmar was conducted to define the presence of antibodies to burkholderia pseudomallei, the cause of melioidosis. antibodies were detectable by indirect hemagglutination assay (iha) in 757 (78%) of 968 adults, of whom 69 (7%) had an iha titer > or =1:160. | 2006 | 17123993 |
| melioidosis in the americas. | melioidosis is a potentially severe bacterial infection caused by burkholderia pseudomallei. there has been growing awareness of the disease in the americas, particularly since the vietnam conflict when it was diagnosed in returning service personnel. accidental laboratory exposure indicates the difficulty making a culture-based diagnosis when melioidosis has not been considered in the differential diagnosis. melioidosis is most likely underdiagnosed in tropical central and south america where c ... | 2006 | 17123994 |
| computed tomography characteristics of burkholderia pseudomallei-associated liver abscess. | 2006 | 17109298 | |
| the mvin homolog in burkholderia pseudomallei is essential for viability and virulence. | the virulence factors of burkholderia pseudomallei, the causative agent of melioidosis, are not fully understood. we have identified a gene with homology to the salmonella typhimurium mouse virulence gene, mvin, a member of the mouse virulence factor family. expression studies with an insertional mutant containing a lux operon demonstrated that the expression of the gene is influenced by free-iron availability in the media and by growth phase. the mutant displayed an increased ld50 value in the ... | 2006 | 17110975 |
| quorum sensing regulates dpsa and the oxidative stress response in burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a fatal human tropical disease. the non-specific dna-binding protein dpsa plays a key role in protecting b. pseudomallei from oxidative stress mediated, for example, by organic hydroperoxides. the regulation of dpsa expression is poorly understood but one possibility is that it is regulated in a cell population density-dependent manner via n-acylhomoserine lactone (ahl)-dependent quorum sensing (qs) since a lux-box motif has been l ... | 2006 | 17159218 |
| fatal burkholderia pseudomallei septicaemia. | 2006 | 17180814 | |
| melioidosis: a potentially life threatening infection. | melioidosis is caused by the gram-negative bacillus, burkholderia pseudomallei. it is endemic in tropical australia and in southeast asian countries. the overall mortality from this infection remains extremely high despite recent advancement in its treatment. this review discuss about clinical manifestations, diagnosis and management of melioidosis. | 2006 | 17240600 |
| mycobacterium avium and burkholderia pseudomallei (melioidosis) coinfection in an hiv-positive patient. | a 29 year old hiv positive thai female with cd4 count of 10 cells/mm3 presented with chronic diffuse abdominal pain, fever, weight loss, anemia and leucopenia. ultrasonography demonstrated diffuse upper abdominal lymphadenopathy with ascites. microbiological and molecular work up of the specimen obtained by ultrasound-guided lymph node aspiration revealed co-infection with burkholderia pseudomallei and mycobacterium avium. indirect hemagglutination, igm-indirect fluorescent antibody, and igg-ind ... | 2006 | 17348247 |
| [cutaneous melioidosis: report of a case]. | 2006 | 17374271 | |
| melioidosis with endophthalmitis. | 2006 | 17030726 | |
| prospective evaluation of a rapid immunochromogenic cassette test for the diagnosis of melioidosis in northeast thailand. | a prospective study was performed to compare a rapid immunochromogenic cassette test (ict) with the indirect haemagglutination assay (iha) and clinical rules for the diagnosis of melioidosis in an endemic area. the sensitivity and specificity of the igg ict was 86% and 47%, and the igm ict was 82% and 47%, respectively. these were similar to the results for iha (sensitivity 73%, specificity 64%) and clinical rules (73% and 37%). ict lacks clinical utility as a result of high background rates of ... | 2006 | 16168447 |
| immunogenicity and anti-burkholderia pseudomallei activity in balb/c mice immunized with plasmid dna encoding flagellin. | plasmid dna encoding the flagella protein (flagellin) was used as a vaccination candidate for the evaluation of its immunogenicity and for protection against infection with burkholderia pseudomallei. firstly, flagellin encoding plasmid dna was injected into balb/c mice intramuscularly and this elicited both a humoral and a cellular immune response. total igg production and the clonal expansion of the spleen cells increased in response to flagellin. the igg subclass response exhibited a dominance ... | 2006 | 16169637 |
| development of a 5'-nuclease real-time pcr assay targeting flip for the rapid identification of burkholderia mallei in clinical samples. | burkholderia mallei is a potential biological agent that causes glanders or farcy in solipeds, a disease notifiable to the office international des epizooties (oie). the number of reported outbreaks has increased steadily during the last decade, but diagnosis is hampered by the low bacterial load in infected tissues and excretions. | 2006 | 16449212 |
| genetic and transcriptional analysis of the siderophore malleobactin biosynthesis and transport genes in the human pathogen burkholderia pseudomallei k96243. | burkholderia pseudomallei is a gram-negative facultative intracellular pathogen that causes melioidosis, an invasive disease of humans and animals. to address the response of this bacterium to iron-limiting conditions, we first performed a global transcriptional analysis of rna extracted from bacteria grown under iron-limiting and iron-rich conditions by microarrays. we focused our study on those open reading frames (orfs) induced under iron limitation, which encoded predicted proteins that coul ... | 2006 | 16452439 |
| indirect hemagglutination assay in patients with melioidosis in northern australia. | melioidosis is caused by the saprophytic organism burkholderia pseudomallei. the use of the indirect hemagglutination assay (iha) has found widespread use in areas endemic for this disease. using this assay, we explored the serologic profile of 275 patients with culture-confirmed melioidosis in the northern territory of australia. based on a threshold titer of 1:40, the sensitivity of the iha on admission was 56%. female patients, those with positive blood cultures, and those with pneumonia inde ... | 2006 | 16474092 |
| quorum sensing in the genus burkholderia. | the genus burkholderia contains over 30 species, many of which are important human pathogens. in addition to the primary pathogens burkholderia pseudomallei and burkholderia mallei, several species have emerged as opportunistic pathogens in persons suffering from cystic fibrosis (cf) and immunocompromised individuals. all burkholderia species investigated so far employ quorum-sensing (qs) systems that rely on n-acyl-homoserine lactone (ahl) signal molecules to express certain phenotypic traits i ... | 2006 | 16490397 |
| cpg-modified plasmid dna encoding flagellin improves immunogenicity and provides protection against burkholderia pseudomallei infection in balb/c mice. | the plasmid dna encoding the flic gene of burkholderia pseudomallei combined with cpg oligodeoxynucleotide (odn) was injected intramuscularly into balb/c mice, resulting in the increased production of certain humoral antibodies and flagellin-specific spleen cell clonal expansion. cpg odn, as an immunoadjuvant, was added to the plasmid containing the flic gene in order to obtain ongoing expression in muscle for a long period. functional expression of flagellin from the constructed cpg-modified pl ... | 2006 | 16495541 |
| protection against heterologous burkholderia pseudomallei strains by dendritic cell immunization. | burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacterium which can cause either chronic infections or acute lethal sepsis in infected individuals. the disease is endemic in southeast asia and northern australia, but little is known about the mechanisms of protective immunity to the bacterium. in this study, we have developed a procedure to utilize dendritic cells in combination with cpg oligodeoxynucleotides as a vaccine delivery vector to induce protective imm ... | 2006 | 16495542 |
| multiple visceral abscess in a case of melioidosis. | 2006 | 16505577 | |
| computed tomography characteristics of burkholderia pseudomallei liver abscess. | we report 3 cases of burkholderia pseudomallei liver abscesses that have a characteristic "honeycomb" appearance on computed tomography scans. such a finding should prompt physicians to include b. pseudomallei infection in the differential diagnoses of liver abscesses and consider initiation of empirical therapy for melioidosis in high-risk patients from areas of endemicity. | 2006 | 16511765 |
| role of t cells in innate and adaptive immunity against murine burkholderia pseudomallei infection. | antigen-specific t cells are important sources of interferon (ifn)-gamma for acquired immunity to intracellular pathogens, but they can also produce ifn- gamma directly via a "bystander" activation pathway in response to proinflammatory cytokines. we investigated the in vivo role of cytokine- versus antigen-mediated t cell activation in resistance to the pathogenic bacterium burkholderia pseudomallei. ifn-gamma, interleukin (il)-12, and il-18 were essential for initial bacterial control in infec ... | 2006 | 16388484 |
| development and evaluation of a real-time pcr assay targeting the type iii secretion system of burkholderia pseudomallei. | here we report on the development of a discriminatory real-time assay for the rapid identification of burkholderia pseudomallei isolates and the evaluation of this assay for sensitivity against related species and detection in spiked human blood samples. the assay targets a 115-base-pair region within orf2 of the b. pseudomallei type iii secretion system gene cluster and distinguishes b. pseudomallei from other microbial species. assay performance was evaluated with 224 geographically, temporall ... | 2006 | 16390953 |
| serological evidence for increased human exposure to burkholderia pseudomallei following the tsunami in southern thailand. | a serological study was performed to determine recent human exposure to burkholderia pseudomallei (the cause of melioidosis) in residents of southern thailand affected by the tsunami of 26 december 2004. the findings were suggestive of increased recent exposure in both tsunami survivors and uninjured bystanders. survivors of the thailand tsunami may be at increased risk of melioidosis. | 2006 | 16390980 |
| melioidotic osteomyelitis treated with antibiotic-calcium hydroxyapatite composite: case report with four-year follow-up. | melioidosis is caused by an infection by burkholderia pseudomallei. osteomyelitis is a recognised manifestation of melioidosis but burkholderia pseudomallei is a relatively rare aetiological agent in musculoskeletal infections. we report a 32-year-old diabetic man with septicaemia due to melioidotic infection of the spleen, liver and distal femur. the osteomyelitis relapsed despite being treated with the standard radical debridement and insertion of gentamycinimpregnated polymethylmetacrylate (p ... | 2006 | 16397726 |
| melioidosis as a cause of multifocal osteomyelitis. | 2006 | 16374119 | |
| use of various common isolation media to evaluate the new vitek 2 colorimetric gn card for identification of burkholderia pseudomallei. | the use of automated systems in the modern microbiology laboratory is becoming commonplace as the pressure of cost containment impacts on staff resources. with increased international travel and threats of bioterrorism, recognition and accurate identification of organisms such as burkholderia pseudomallei is important. in areas where this organism is endemic, identification is not usually problematic. this study evaluates the performance of the new vitek 2 colorimetric gn card for the identifica ... | 2006 | 16517866 |
| [molecular typing of the burkholderia pseudomallei strains with different resistance to antibiotics]. | wild type strains of burkholderia pseudomallei, spontaneous mutants with high resistance to fluoroquinolones and ceftazidime, and tn5-induced mutants with reduced resistance level were studied using polymorphic and gene-specific dna fingerprinting. cluster analysis of genomic dna patterns obtained using pcr with arbitrary primer (5'-gtttcgctcc-3') and primer specific to the class i integrase intll gene (5'-cctcccgcacgatgatc-3') was performed. according to the dna pattern conformity, the distinct ... | 2006 | 16512603 |
| extreme weather events and environmental contamination are associated with case-clusters of melioidosis in the northern territory of australia. | melioidosis, the infection due to the environmental organism burkholderia pseudomallei, is endemic to northern australia and south east asia. it is associated with exposure to mud and pooled surface water, but environmental determinants of this disease are poorly understood. we defined case-clusters in northern australia, determined their contribution to the observed rate of melioidosis, and explored clinical features and associated environmental factors. | 2006 | 16326823 |
| burkholderia pseudomallei infection in chronic granulomatous disease. | melioidosis is a severe illness caused by burkholderia pseudomallei, a sepsis-causing bacterial pathogen that is common in east asia. we reexamined the underlying diagnosis in a 12-year-old boy who was diagnosed in the french west indies with melioidosis when he was 4 years old. our investigations led to the determination of chronic granulomatous disease (cgd), an inherited condition characterized by phagocytic cell dysfunction. this is the third reported case of melioidosis associated with cgd ... | 2006 | 16328364 |
| actin-dependent movement of bacterial pathogens. | listeria, rickettsia, burkholderia, shigella and mycobacterium species subvert cellular actin dynamics to facilitate their movement within the host cytosol and to infect neighbouring cells while evading host immune surveillance and promoting their intracellular survival. 'attaching and effacing' escherichia coli do not enter host cells but attach intimately to the cell surface, inducing motile actin-rich pedestals, the function of which is currently unclear. the molecular basis of actin-based mo ... | 2006 | 16415925 |
| melioidosis: insights into the pathogenicity of burkholderia pseudomallei. | burkholderia pseudomallei is a potential bioterror agent and the causative agent of melioidosis, a severe disease that is endemic in areas of southeast asia and northern australia. infection is often associated with bacterial dissemination to distant sites, and there are many possible disease manifestations, with melioidosis septic shock being the most severe. eradication of the organism following infection is difficult, with a slow fever-clearance time, the need for prolonged antibiotic therapy ... | 2006 | 16541135 |
| endotoxin-like properties of a rhamnolipid exotoxin from burkholderia (pseudomonas) plantarii: immune cell stimulation and biophysical characterization. | here we report on the purification, structural characterization, and biological activity of a glycolipid, 2-o-alpha-l-rhamnopyranosyl-alpha-l-rhamnopyranosyl-alpha(r)-3-hydroxytetradecanoyl-(r)-3-hydroxytetradecanoate (rl-2,2(14)) produced by burkholderia (pseudomonas) plantarii. rl-2,2(14) is structurally very similar to a rhamnolipid exotoxin from pseudomonas aeruginosa and identical to the rhamnolipid of burkholderia pseudomallei, the causative agent of melioidosis. interestingly, rl-2,2(14) ... | 2006 | 16542152 |
| six cases of suppurative lymphadenitis caused by burkholderia pseudomallei infection. | melioidosis, an infection caused by burkholderia pseudomallei, is endemic in southeast asia and northern australia. it can have various presentations, ranging from acute septicaemia to chronic localized infection. uncomplicated, localized infection usually involves skin or subcutaneous tissues. suppurative lymphadenitis caused by melioidosis has been rarely encountered by clinicians practicing in endemic areas. we encountered six cases of suppurative lymphadenitis caused by burkholderia pseudoma ... | 2006 | 16542691 |
| development of a polymerase chain reaction assay for the specific identification of burkholderia mallei and differentiation from burkholderia pseudomallei and other closely related burkholderiaceae. | burkholderia mallei and burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category b biothreat agents. we used an in silico approach to compare the b. mallei atcc 23344 and b. pseudomallei k96243 genomes to identify nucleotide sequences unique to b. mallei. five distinct b. mallei dna sequences and/or genes were identified and evaluated for polymerase chain reaction (pcr) assay development. g ... | 2006 | 16546342 |
| clinical guideline for diagnosis and management of melioidosis. | melioidosis is an emerging infection in brazil and neighbouring south american countries. the wide range of clinical presentations include severe community-acquired pneumonia, septicaemia, central nervous system infection and less severe soft tissue infection. diagnosis depends heavily on the clinical microbiology laboratory for culture. burkholderia pseudomallei, the bacterial cause of melioidosis, is easily cultured from blood, sputum and other clinical samples. however, b. pseudomallei can be ... | 2006 | 16547571 |
| stent-graft for recurrent melioidosis mycotic aortic aneurysm. | melioidosis is a tropical disease caused by burkholderia pseudomallei and is prevalent in south east asia and northern australia. it can infect any organ system and is potentially deadly. melioidosis causing a mycotic aneurysm of the aorta is rare. we present a patient with a melioidosis mycotic aneurysm of the descending aorta presenting with fever and right pleural effusion, managed successfully with initial dacron graft repair with staged omental reinforcement, and subsequent endovascular ste ... | 2006 | 16551809 |
| in vitro activity of tigecycline against burkholderia pseudomallei and burkholderia thailandensis. | investigation of the in vitro activity of tigecycline against burkholderia pseudomallei and burkholderia thailandensis revealed that the inhibition zone diameters of tigecycline against all isolates were >or=20 mm and that the mic50 values were 0.5 and 1 microg/ml and the mic90 values were 2 and 1.5 microg/ml for b. pseudomallei and b. thailandensis, respectively. | 2006 | 16569883 |
| dural sinus thrombosis in melioidosis: the first case report. | melioidosis which is infection with burkholderia pseudomallei, is an important cause of sepsis in india, southeast asia and northern australia. mortality is high and treatment is problematic. neurological melioidosis is unusual but meningoencephalitis, encephalomyelitis and brain microabscess can occur dural sinus thrombosis is not an uncommon cerebrovascular disorder with various etiologies. hypercoagulable state, pregnancy, dehydration, certain blood dyscrasia and contraceptive pills are commo ... | 2006 | 16579013 |
| using real-time pcr to specifically detect burkholderia mallei. | burkholderia mallei is the causative agent of human and animal glanders and is a category b biothreat agent. rapid diagnosis of b. mallei and immediate prophylactic treatment are essential for patient survival. the majority of current bacteriological and immunological techniques for identifying b. mallei from clinical samples are time-consuming, and cross-reactivity with closely related organisms (i.e. burkholderia pseudomallei) is a problem. in this investigation, two b. mallei-specific real-ti ... | 2006 | 16585642 |
| use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of burkholderia pseudomallei. | melioidosis, a disease caused by the bacterium burkholderia pseudomallei, is endemic in southeast asia and northern australia. we used suppression subtractive hybridization (ssh) to identify sequences that varied between two b. pseudomallei isolates from australia and determined the distribution of 45 ssh-derived sequences among a panel of b. pseudomallei and b. thailandensis isolates. sequences exhibiting variable prevalence were included in a variable amplicon typing (vat) scheme designed to s ... | 2006 | 16597858 |
| the impact of homologous recombination on the generation of diversity in bacteria. | the imprint of demographic and selective processes on bacterial population structure needs to be evaluated as deviation from the expectations of an appropriate null neutral model. we explore the impact of varying the population mutation and recombination rates theta and rho on ideal populations, using a recently developed model of neutral drift at multiple loci. this model may be fitted to experimental data to provide estimates of these parameters, and we do so for seven bacterial species (neiss ... | 2006 | 16236325 |
| decreased protein c, protein s, and antithrombin levels are predictive of poor outcome in gram-negative sepsis caused by burkholderia pseudomallei. | acute septicemic melioidosis is associated with systemic release of endotoxin and the proinflammatory cytokines tumor necrosis factor (tnf)-alpha, interleukin-1, and interleukin-6. excessive release of these cytokines may lead to endothelial injury, depletion of naturally occurring endothelial modulators, microvascular thrombosis, organ failure, and death. | 2006 | 16290015 |
| cutaneous melioidosis in a swedish tourist after the tsunami in 2004. | a tourist from sweden developed cutaneous melioidosis after the tsunami in thailand on 26 december 2004. melioidosis is a severe, chronic infection which is endemic in thailand and is caused by burkholderia pseudomallei. persons with traumatic injuries inflicted by the tsunami have increased risks of being infected by b. pseudomallei and melioidosis should be suspected if abscesses of the skin or inner organs develop in the months or years after the trauma. | 2006 | 16338844 |
| rapid molecular typing of burkholderia pseudomallei, isolated in an outbreak of melioidosis in singapore in 2004, based on variable-number tandem repeats. | an increase in the number of reported melioidosis cases was observed in the first 4 months of 2004. these cases were associated with a significant increase in case-fatality rate compared with the past 5 years. in order to exclude the possibility of a single source, including the possibility of intentional release of burkholderia pseudomallei, we applied a multiplex pcr-based multilocus variable-number tandem repeat (vntr) assay to determine the clonality of the clinical isolates. our investigati ... | 2006 | 16343569 |
| polysaccharides and virulence of burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, an infectious disease of humans and animals. gene clusters which encode capsular polysaccharide (type i o-ps) and lps (type ii o-ps), both of which play roles in virulence, have previously been identified. here, the identification of two further putative clusters, type iii o-ps and type iv o-ps, is reported. mice challenged with type iii o-ps or type iv o-ps mutants showed increased mean times to death (7.8 and 11.6 days) compared ... | 2007 | 17644705 |
| [the use of fluorescent liposomal immunoanalysis for the diagnostics and indication of glanders and melioidosis]. | the authors have developed a homogenous method for the detection of the cells, surface antigen, and ag8 of glanders and melioidosis pathogens and antibodies to them, based on the compliment-dependent lysis of ag8-sensitized liposomes. the method is highly specific; its sensitivity to antibodies to glanders and melioidosis pathogens is 24 to 440 times higher than that of rid: its sensitivity to ag8 is 100 ng/ml corresponding to 1.25x10-10 m, and its sensitivity to cells is 10(5) to 10(6) cells/ml ... | 2007 | 18228662 |
| atypical presentations of melioidosis as emerging threat: a case report. | we report two atypical presentations of melioidosis as mediastinal lymphadenitis and prostatic abscess with burkholderia pseudomallei, the emerging category 2 organism which led to diagnostic and therapeutic dilemma and thereby, delay in appropriate management. any similar presentation should always be supported by microbiological opinion without any delay, which can help in instituting proper antibiotics with successful outcome. | 2007 | 18306611 |
| survival of burkholderia pseudomallei on environmental surfaces. | the survival of the biothreat agent burkholderia pseudomallei on the surfaces of four materials was measured by culture and esterase activity analyses. the culture results demonstrated that this organism persisted for <24 h to <7 days depending on the material, bacterial isolate, and suspension medium. the persistence determined by analysis of esterase activity, as measured with a scanrdi solid-phase cytometer, was always longer than the persistence determined by culture analysis. | 2007 | 17951437 |
| osteomyelitis of parietal bone in melioidosis. | 2007 | 17953108 | |
| spectra of central nervous system melioidosis. | burkholderia pseudomallei infection of the central nervous system (cns) is rare with less than 50 cases reported over the last 30 years. the retrospective melioidosis study at university malaya medical centre has documented three cases of cns melioidosis out of more than 160 cases of melioidosis since 1978. there were two patients with brain abscess and one with spinal epidural abscess. the predisposing factors were: one patient was an aboriginal farmer and the other two were diabetic. their age ... | 2007 | 17964168 |
| lymphocytic pleural effusion in acute melioidosis. | an endemic outbreak of melioidosis developed in southern taiwan following a flood caused by a typhoon in july 2005. a total of 27 patients were diagnosed with the acute and indigenous form of pulmonary melioidosis. parapneumonic pleural effusions were noted on chest x-rays in six patients. thoracentesis was done in three patients and all revealed lymphocyte predominance in differential cell count. burkholderia pseudomallei was isolated in the pleural effusion in one of them. all three patients s ... | 2007 | 17964969 |
| pacemaker infection secondary to burkholderia pseudomallei. | infection is a relatively rare but devastating complication of intracardiac device implantation. burkholderia pseudomallei is the organism which causes melioidosis, an endemic and lethal infection in the tropics. we describe a case of pacemaker infection secondary to burkholderia pseudomallei, which was treated by explantation of the device and appropriate antimicrobial therapy. | 2007 | 17976112 |
| burkholderia rhizoxinica sp. nov. and burkholderia endofungorum sp. nov., bacterial endosymbionts of the plant-pathogenic fungus rhizopus microsporus. | several strains of the fungus rhizopus microsporus harbour endosymbiotic bacteria for the production of the causal agent of rice seedling blight, rhizoxin, and the toxic cyclopeptide rhizonin. r. microsporus and isolated endobacteria were selected for freeze-fracture electron microscopy, which allowed visualization of bacterial cells within the fungal cytosol by their two parallel-running envelope membranes and by the fine structure of the lipopolysaccharide layer of the outer membrane. two repr ... | 2007 | 17978222 |
| quantitation of b. pseudomallei in clinical samples. | we undertook a prospective study to quantitate burkholderia pseudomallei in blood, urine, respiratory secretions, and pus [corrected] obtained from 414 patients with melioidosis. the median was count 1.1, 1.5 x 10(4), 1.1 x 10(5), and 1.1 x 10(7) cfu/ml in these sample types, respectively. this provides important insights into the likely feasibility of future studies such as expression microarray analysis using clinical material. | 2007 | 17984331 |
| prospective clinical evaluation of the accuracy of 16s rrna real-time pcr assay for the diagnosis of melioidosis. | the accuracy of a burkholderia pseudomallei 16s rrna real-time pcr assay was evaluated against culture for the diagnosis of melioidosis in thailand. a total of 846 samples were obtained from 383 patients with suspected melioidosis. one or more specimens were pcr positive for 47 of 77 patients with culture-proven melioidosis (sensitivity 61.0%, 95% ci: 49.2-72.0%). pcr was negative for all 306 patients who were culture negative for b. pseudomallei (specificity 100%, 95% ci: 98.8-100%). diagnostic ... | 2007 | 17984332 |
| virulence of clinical and environmental isolates of burkholderia oklahomensis and burkholderia thailandensis in hamsters and mice. | burkholderia pseudomallei is the etiologic agent of the tropical disease melioidosis and is considered to be a potential biological weapon. two b. pseudomallei-like species, burkholderia oklahomensis and burkholderia thailandensis, have been described in the literature. infections with both of these microorganisms have occurred in the united states, but little is known about the relative virulence of these isolates in animal models of infection. in this study, b. oklahomensis and b. thailandensi ... | 2007 | 17986086 |
| [mission oriented diagnostic real-time pcr]. | in out of area military missions soldiers are potentially exposed to bacteria that are endemic in tropical areas and can be used as biological agents. it can be difficult to culture these bacteria due to sample contamination, low number of bacteria or pretreatment with antibiotics. commercial biochemical identification systems are not optimized for these agents which can result in misidentification. immunological assays are often not commercially available or not specific. real-time pcr assays a ... | 2007 | 17987355 |
| expression profile and function of triggering receptor expressed on myeloid cells-1 during melioidosis. | triggering receptor expressed on myeloid cells-1 (trem-1) amplifies toll-like receptor-initiated responses against pathogens. we aimed to characterize trem-1 expression and function during sepsis caused by burkholderia pseudomallei (melioidosis). | 2007 | 18008257 |
| genome-wide prediction and annotation of burkholderia pseudomallei arac/xyls family transcription regulator. | many members of the arac/xyls family transcription regulator have been proven to play a critical role in regulating bacterial virulence factors in response to environmental stress. by using the hidden markov model (hmm) profile built from the alignment of a 99 amino acid conserved domain sequence of 273 arac/xyls family transcription regulators, we detected a total of 45 arac/xyls family transcription regulators in the genome of the gram-negative pathogen, burkholderia pseudomallei. further in s ... | 2007 | 18391231 |
| [fluoroquinolones: antimicrobial activity and chemotherapeutic efficacy with respect to various pathogens in highly dangerous diseases]. | comparative in vitro and in vivo efficacy of new fluoroquinolones with respect to pathogens of tularemia, glanders, melioidosis and anthrax was estimated. it was shown that the strains of the tularemia, glanders, melioidosis and anthrax pathogens were in vitro highly susceptible to the new agents. the experiments on laboratory animals demonstrated that pefloxacin and sparfloxacin had extremely broad spectra and were of special value in emergency prophylaxis of tularemia, glanders, melioidosis an ... | 2007 | 19275053 |
| to catch a killer gene. | 2007 | 17380132 | |
| control of quorum sensing by a burkholderia pseudomallei multidrug efflux pump. | the burkholderia pseudomallei khw quorum-sensing systems produced n-octanoyl-homoserine lactone, n-decanoyl-homoserine lactone, n-(3-hydroxy)-octanoyl-homoserine lactone, n-(3-hydroxy)-decanoyl-homoserine lactone, n-(3-oxo)-decanoyl-homoserine lactone, and n-(3-oxo)-tetradecanoyl-homoserine lactone. the extracellular secretion of these acyl-homoserine lactones is dependent absolutely on the function of the b. pseudomallei bpeab-oprb efflux pump. | 2007 | 17384185 |
| paediatric melioidosis with septic shock in a previously-well child. | we present a previously-healthy 12-year old girl from a rural community and who was admitted to a district general hospital in malaysia with coagulopathy and septic shock. despite receiving intensive care, she succumbed to her illness. blood cultures grew burkholderia pseudomallei. melioidosis is an unusual cause of paediatric gram-negative sepsis among children in malaysia. | 2007 | 17384864 |
| atp-binding cassette systems in burkholderia pseudomallei and burkholderia mallei. | atp binding cassette (abc) systems are responsible for the import and export of a wide variety of molecules across cell membranes and comprise one of largest protein superfamilies found in prokarya, eukarya and archea. abc systems play important roles in bacterial lifestyle, virulence and survival. in this study, an inventory of the abc systems of burkholderia pseudomallei strain k96243 and burkholderia mallei strain atcc 23344 has been compiled using bioinformatic techniques. | 2007 | 17391530 |
| human single-chain fv antibodies against burkholderia mallei and burkholderia pseudomallei. | much effort has been devoted to the development of mouse monoclonal antibodies that react specifically with burkholderia mallei and burkholderia pseudomallei for diagnostic and/or therapeutic purposes. our present study focused on the screening of a phage-displayed nonimmune human single-chain fv (scfv) antibody library against heat-killed b. mallei and b. pseudomallei for the generation of human scfv antibodies specific to the two pathogenic species of bacteria. using two different panning proc ... | 2007 | 17392491 |
| tandem repeat regions within the burkholderia pseudomallei genome and their application for high resolution genotyping. | the facultative, intracellular bacterium burkholderia pseudomallei is the causative agent of melioidosis, a serious infectious disease of humans and animals. we identified and categorized tandem repeat arrays and their distribution throughout the genome of b. pseudomallei strain k96243 in order to develop a genetic typing method for b. pseudomallei. we then screened 104 of the potentially polymorphic loci across a diverse panel of 31 isolates including b. pseudomallei, b. mallei and b. thailande ... | 2007 | 17397553 |
| imported melioidosis with an isolated cutaneous presentation in a 90-year-old traveller from bangladesh. | melioidosis is a tropical disease caused by infection with the bacterium burkholderia pseudomallei. most cases present as an acute febrile illness with severe pneumonia and sepsis. sub-acute and late onset disease can also occur melioidosis has been diagnosed among travellers who contracted the disease while staying in endemic areas during the rainy season. we report a case of travel-associated b. pseudomallei cutaneous infection in a febrile 90-year-old woman with diabetes mellitus, with early ... | 2007 | 17402688 |
| use of protein-specific monoclonal antibody-based latex agglutination for rapid diagnosis of burkholderia pseudomallei infection in patients with community-acquired septicemia. | a latex agglutination test employing monoclonal antibody specific to a 30-kda protein of burkholderia pseudomallei was used to detect the organisms in blood culture specimens from 1,139 patients with community-acquired septicemia. the sensitivity, specificity, and positive and negative predictive values of the test were 96.75%, 99.61%, 96.75%, and 99.61%, respectively. | 2007 | 17428952 |
| assessing the reliability of eburst using simulated populations with known ancestry. | the program eburst uses multilocus sequence typing data to divide bacterial populations into groups of closely related strains (clonal complexes), predicts the founding genotype of each group, and displays the patterns of recent evolutionary descent of all other strains in the group from the founder. the reliability of eburst was evaluated using populations simulated with different levels of recombination in which the ancestry of all strains was known. | 2007 | 17430587 |
| use of immunoblotting as an alternative method for serogrouping leptospira. | leptospirosis is a worldwide zoonotic disease caused by a spirochaete bacterium, leptospira. serological detection of this micro-organism basically relies on a conventional microscopic agglutination test (mat), which has some limitations and disadvantages. in the present study, immunoblotting has been applied as an alternative method for differentiating serogroups and serovars of leptospires. leptospiral whole-cell lysates from a total of 26 serovars were subjected to immunoblotting using rabbit ... | 2007 | 17446278 |
| melioidotic septic arthritis: a case report and literature review. | burkholderia pseudomallei, the causative agent of melioidosis, is endemic in southeast asia and northern australia. in recent years, the incidence of melioidosis has increased worldwide. septic arthritis is a rare but well-recognized manifestation of melioidosis. patients with underlying medical conditions, such as diabetes mellitus, renal impairment, cirrhosis, and malignancy are at greater risk. the presentations of melioidotic septic arthritis often mimic other disease processes and patients ... | 2007 | 17446969 |
| melioidosis complicated by pericarditis. | melioidosis is a bacterial infection caused by the gram-negative bacillus burkholderia pseudomallei. we report an unusual case of melioidosis that presented as a pyrexia of unknown origin complicated by pericardial effusion. our patient received a 6-week course of intravenous antibiotics, followed by 8 months of oral antibiotics, and made a complete recovery. this report illustrates the diagnostic and therapeutic challenge that clinicians may encounter when faced with this potentially fatal infe ... | 2007 | 17454903 |
| characteristics of phenol biodegradation in saline solutions by monocultures of pseudomonas aeruginosa and pseudomonas pseudomallei. | phenol is a highly toxic and carcinogenic compound and its biodegradation is very important to meet the environmental regulations. two bacterial strains capable of utilizing phenol as a sole source of carbon were isolated from the wastewater of a pharmaceutical industry. on the basis of morphological and biochemical characteristics these strains were identified as pseudomonas aeruginosa and pseudomonas pseudomallei. both of these strains were very efficient for phenol degradation. p. pseudomalle ... | 2007 | 17459580 |