Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the detection of porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans in the supragingival plaque of children with and without caries. | the present study was undertaken to determine the presence of 3 periodontopathic bacteria in the supragingival plaques of 3- to 16-year-old children with different oral health conditions. | 2003 | 12723840 |
| fluorescence in situ hybridization (fish) for direct visualization of bacteria in periapical lesions of asymptomatic root-filled teeth. | whether micro-organisms can live in periapical endodontic lesions of asymptomatic teeth is under debate. the aim of the present study was to visualize and identify micro-organisms within periapical lesions directly, using fluorescence in situ hybridization (fish) in combination with epifluorescence and confocal laser scanning microscopy (clsm). thirty-nine periapical lesions were surgically removed, fixed, embedded in cold polymerizing resin and sectioned. the probe eub 338, specific for the dom ... | 2003 | 12724371 |
| adhesins encoded by the gingipain genes of porphyromonas gingivalis are responsible for co-aggregation with prevotella intermedia. | co-aggregation among bacterial cells caused by the adherence of one bacterial species to another is a potential colonization mechanism. several putative aggregation factors for co-aggregation between porphyromonas (por.) gingivalis and prevotella (pre.) intermedia were partially purified from por. gingivalis vesicles by gel filtration and affinity chromatography. antisera against the aggregation factors were made. analysis using these antisera revealed that 18 and 44 kda proteins might be respon ... | 2003 | 12724387 |
| cleavage of cd14 and lbp by a protease from prevotella intermedia. | periodontitis is an inflammatory disease caused by subgingival microorganisms and their components, such as lipopolysaccharide (lps). responses of the host to lps are mediated by cd14 and lps-binding protein (lbp). in this study, it was determined that proteases from a periodontal pathogen, prevotella intermedia, cleave cd14 and lbp, and thereby modulate the virulence of lps. culture supernatants from two strains of p. intermedia (atcc 25611 and 25261) cleaved cd14 and lbp in a concentration-dep ... | 2003 | 12728301 |
| effect of black-pigmented bacteria on the plasminogen-plasmin system in human pulp and osteoblastic cells. | to date, there have been relatively few studies addressing the presence and expression of the plasminogen-plasmin system at the site of bacterial infection. the aim of the present study was to investigate the effect of black-pigmented bacteria on the expression of the plasminogen-plasmin system in human pulp and osteoblastic cells. | 2003 | 12738955 |
| induction of interleukin-6 gene expression by pro-inflammatory cytokines and black-pigmented bacteroides in human pulp cell cultures. | to investigate the effect of pro-inflammatory cytokines and black-pigmented bacteroides on the expression of il-6 gene in human pulp fibroblasts. | 2003 | 12752649 |
| accidentally induced periodontitis in primary dentition: longitudinal examinations of periodontal bacteria and clinical conditions. | periodontitis is very rare in the primary dentition although it can be accidentally induced in young children. the purpose of this study was to describe a case of periodontitis in a 4-year-old child, which was accidentally induced by insertion of small plastic tubes into the dental cervix of the primary incisors during play, and to discuss the clinical, radiographic, and microbiological findings. removal of the plastic tubes resulted in resolution of the periodontal condition. prevotella nigresc ... | 2003 | 12752919 |
| generation of gingival t cell lines/clones specific with porphyromonas gingivalis pulsed dendritic cells from periodontitis patients. | it is well documented that in periodontitis lesions, most infiltrated gingival t cells are antigen-specific memory t cells. these cells play an important role as regulators and effector cells in the pathogenesis of periodontitis. in this study, we used dendritic cells (dcs) as antigen-presenting cells to generate human gingival t cell lines and clones specific for porphyromonas gingivalis from periodontitis patients. | 2003 | 12753363 |
| inhibitory effects of green tea catechins on protein tyrosine phosphatase in prevotella intermedia. | members of the prevotella intermedia group possess protein tyrosine phosphatase (ptpase). the purpose of this study was to investigate the effects of catechin derivatives from japanese green tea on the activity of ptpase in p. intermedia and related organisms. multilocus enzyme electrophoresis of alkaline phosphatase derived from p. intermedia, prevotella nigrescens, prevotella pallens and porphyromonas gingivalis revealed a species-specific migration pattern. among the tea catechin derivatives ... | 2003 | 12753472 |
| purification and partial characterization of a dipeptidyl peptidase from prevotella intermedia. | a peptidase hydrolyzed x-pro-p-nitroanilide was purified from the cell extract of prevotella intermedia atcc 25611 by ion-exchange chromatography and hydrophobic interaction chromatography. the purified enzyme exhibited a molecular size of 74 kda from sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the maximum enzyme activity was found between ph 7.0 and ph 7.5. this peptidase was a serine enzyme and hydrolyzed lys-pro-p-nitroanilide, arg-pro-p-nitroanilide, and ala-pro-p-nitroanil ... | 2003 | 12753473 |
| chemical structure and immunobiological activity of lipid a from prevotella intermedia atcc 25611 lipopolysaccharide. | the novel chemical structure and immunobiological activities of prevotella intermedia atcc 25611 lipid a were investigated. a lipopolysaccharide (lps) preparation of p. intermedia was extracted using a phenol-chloroform-petroleum ether method, after which its purified lipid a was prepared by weak acid hydrolysis followed by chromatographic separations. the lipid a structure was determined by mass spectrometry and nuclear magnetic resonance to be a diglucosamine backbone with a phosphate at the 4 ... | 2003 | 12753913 |
| comparison of laboratory methods for detecting beta-lactamase-positive strains in the species prevotella intermedia sensu lato isolated from periodontal pockets. | 2003 | 12783282 | |
| prevalence of periodontal bacterial infection in non-obese japanese type 2 diabetic patients: relationship with c-reactive protein and albuminuria. | the aim of the present study was to investigate the relationship between periodontal bacteria infection ( porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedius) and c-reactive protein (crp) and albuminuria in non-obese japanese type 2 diabetic patients. one hundred and thirty-four non-obese japanese type 2 diabetic patients without evidence of current acute illness including clinically significant acute infectious disease were enrolled into the study. the degree ... | 2004 | 15002063 |
| quadrant root planing versus same-day full-mouth root planing. | the aim of this study was to determine whether same-day full-mouth scaling and root planing (fm-srp) and quadrant scaling and root planing (q-srp) resulted in variations in the systemic humoral immune response dynamics (antibody titres and avidity) during active treatment and 3 and 6 months post-therapy. | 2004 | 15016017 |
| debridement and local application of tetracycline-loaded fibres in the management of persistent periodontitis: results after 12 months. | the aim of our study was to evaluate the clinical, radiological and microbiological response to the local delivery of tetracycline (te) of sites with persistent periodontal lesions. | 2004 | 15016019 |
| quadrant root planing versus same-day full-mouth root planing. ii. microbiological findings. | the aim of this study was to test the hypothesis that over a period of 6 months, same-day full-mouth scaling and root planing (fm-srp) resulted in greater reductions in the detection frequency of five putative periodontal pathogens compared with quadrant scaling and root planing (q-srp) in chronic periodontitis patients. | 2004 | 15016040 |
| specific antibiotics in the treatment of periodontitis--a proposed strategy. | the purpose of the present study was to propose a strategy for the selection of antibiotics that specifically target complexes of periodontal pathogens present in patients with periodontitis. | 2004 | 15025229 |
| cervical spinal epidural abscess and meningitis due to prevotella oris and peptostreptococcus micros after retropharyngeal surgery. | 2004 | 15034651 | |
| geographical differences in bacteria detected in endodontic infections using polymerase chain reaction. | the polymerase chain reaction (pcr) is an innovative nucleic acid-based assay that has the highest sensitivity of any microbiological technique for the detection of bacteria. the purpose of this study was to use pcr to detect the presence of specific species of bacteria in samples collected from two geographical locations. microbial samples from abscesses of endodontic origin were collected from patients in portland, oregon, and rio de janeiro, brazil. pcrs with species-specific oligonucleotide ... | 2004 | 15055430 |
| risk factors for periodontitis in hiv patients. | the purpose of this study was to identify risk factors for periodontitis associated with human immunodeficiency virus (hiv) infection. | 2004 | 15102043 |
| quantitative detection of periodontal pathogens using real-time polymerase chain reaction with taqman probes. | quantitative analysis, with identification of periodontopathic bacteria, is important for the diagnosis, therapeutic evaluation and risk assessment of periodontal disease. we developed a highly sensitive and specific method using real-time polymerase chain reaction (pcr) to detect and quantify six periodontal bacteria: porphyromonas gingivalis, tannerella forsythia, actinobacillus actinomycetemcomitans, treponema denticola, prevotella intermedia, and prevotella nigrescens. species-specific taqma ... | 2004 | 15107068 |
| the microbial synergy of peptostreptococcus micros and prevotella intermedia in a murine abscess model. | this study characterized the microbial interaction of peptostreptococcus micros and prevotella intermedia, the major pathogens of dentoalveolar infection, using a murine model. subcutaneous injection of p. micros cells in the dorsum of the mouse together with living cells of p. intermedia resulted in a significantly larger abscess when compared with single injection of the organisms (p < 0.02). the abscess size was also significantly increased (p < 0.05) when the plate-cultured cell suspension o ... | 2004 | 15107069 |
| induction of calprotectin release by porphyromonas gingivalis lipopolysaccharide in human neutrophils. | calprotectin, a major cytosolic protein of leukocytes, is detected in neutrophils, monocytes/macrophages, and epithelial cells. this protein is known to be a marker for several inflammatory diseases and is detected in inflammatory gingival tissue with periodontal disease. recently, we found that the calprotectin level in gingival crevicular fluid from periodontitis patients was significantly higher than that of healthy subjects. however, the regulation of calprotectin in periodontal disease is u ... | 2004 | 15107070 |
| comparison of type 1 and type 2 cytokine production by mononuclear cells cultured with streptococcus mutans and selected other caries bacteria. | a feature of pulpal immune responses is the predominance of type 1 cytokine mrna under shallow caries and a mixed (type 1/type 2) profile under deep caries. these results prompted an examination of the cytokine profiles induced by bacteria in shallow caries (streptococcus mutans and actinomyces viscosus) and deep caries (lactobacillus casei, pseudoramibacter alactolyticus, and prevotella intermedia). all isolates induced interferon-gamma and interleukin-10, whereas interleukin-4 and interleukin- ... | 2004 | 15107645 |
| effects of eight antibacterial agents on cell survival and expression of epithelial-cell- or cell-adhesion-related genes in human gingival epithelial cells. | our previous studies suggest that little adverse effect on the growth of the periodontal ligament would be expected, if tetracycline, minocycline, ofloxacin, roxithromycin, clarithromycin, and azithromycin were topically administered to the periodontal pocket at their mic90 doses required to inhibit the growth of 90% of periodontopathic bacteria, including porphyromonas gingivalis, prevotella intermedia, and actinobacillus actinomycetemcomitans. in the present study, we investigated the cytocida ... | 2004 | 14687228 |
| in vitro evaluation of the antimicrobial activity of chlorhexidine and sodium hypochlorite. | the aim of this study was to investigate in vitro the antimicrobial activity of 0.2%, 1%, and 2% chlorhexidine gluconate (chx gel and chx liquid), against endodontic pathogens and compare the results with the ones achieved by 0.5%, 1%, 2.5%, 4%, and 5.25% sodium hypochlorite (naocl). a broth dilution test was performed, and the timing for irrigants to kill microbial cells was recorded and statistically analyzed. both 2.0% gel and liquid formulations eliminated staphylococcus aureus and candida a ... | 2004 | 14716261 |
| in vitro activation of the hemolysin in prevotella nigrescens atcc 33563 and prevotella intermedia atcc 25611. | hemolytic activity was evaluated in the putative periodontopathogens prevotella intermedia and prevotella nigrescens. whole cells of both species present weak hemolytic activity evidenced only by solid media assays after 48 h of bacterial growth or after 5 h of interaction with erythrocytes at 37 degrees c in liquid assays. in this work we show that the use of crude extract allowed the detection of a higher hemolytic activity for p. intermedia, but surprisingly not for p. nigrescens. incubation ... | 2004 | 14759706 |
| selected endodontic pathogens in the apical third of infected root canals: a molecular investigation. | bacteria located at the apical portion of the root canals are conceivably in a strategic position to induce damage to the periradicular tissues and resulting inflammatory diseases. this study sought to investigate the prevalence of 11 selected putative endodontic pathogens in the apical third of infected root canals associated with periradicular lesions. the apical root portion of 23 extracted teeth with carious pulpal exposures and attached periradicular lesions was sectioned, and the root cana ... | 2004 | 15329567 |
| relationship of subgingival and salivary microbiota to gingival overgrowth in heart transplant patients following cyclosporin a therapy. | severe gingival overgrowth (go) is induced in patients taking cyclosporin a (csa) following organ transplantation. determining which patient will develop go is still not possible. the purpose of this study was to establish an association between csa and gingival overgrowth in heart transplant patients taking into account periodontal and microbiological conditions. | 2004 | 15341348 |
| localized aggressive periodontitis in a six-year-old: a case report. | the purpose of this report was to describe an approach to diagnose and effectively treat a pediatric patient with localized aggressive periodontitis. a 6-year-old female presented with clinical and radiographic evidence of severe attachment loss around several primary teeth. she had no history of systemic disease, periodontal disease, or caries prior to the periodontal abscess that prompted her referral. routine immunological tests did not reveal any functional defects, but dna testing for perio ... | 2004 | 15344629 |
| clinical and microbiologic changes after removal of orthodontic appliances. | the goal of this study was to evaluate the clinical and microbiological factors associated with orthodontic appliances during an episode of gingival inflammation and the impact of appliance removal on periodontal health. this prospective study included 10 patients, aged 12 to 20 years, with clinical signs of gingival inflammation at the final phase of orthodontic treatment (appliance removal). plaque index, gingival index, and probing depth were evaluated, and microbiological samples were collec ... | 2004 | 15356501 |
| clinical and microbiological changes associated with the use of combined antimicrobial therapies to treat "refractory" periodontitis. | the present investigation examined clinical and microbial changes after a combined aggressive antimicrobial therapy in subjects identified as "refractory" to conventional periodontal therapy. | 2004 | 15367191 |
| contribution of periodontal pathogens on tongue dorsa analyzed with real-time pcr to oral malodor. | oral malodor is considered to originate primarily from tongue microbiota populations. however, the relationship between oral malodor and tongue microbiota remains unclear. in this study, tongue periodontal pathogens were analyzed via real-time pcr, and the association between oral malodor and tongue periodontal pathogens, including porphyromonas gingivalis, tannerella forsythia, prevotella intermedia, prevotella nigrescens and treponema denticola, was examined. the subject population consisted o ... | 2004 | 15380777 |
| possible periodontal pathogens associated with clinical symptoms of periodontal disease in japanese high school students. | the purpose of this study is to investigate how the components of biofilm and clinical oral status change in adolescents and to identify specific periodontal pathogens as risk markers for the onset of periodontitis. | 2004 | 15455735 |
| clinical and microbiological effects of topical subgingival application of hyaluronic acid gel adjunctive to scaling and root planing in the treatment of chronic periodontitis. | hyaluronic acid (ha) has shown anti-inflammatory effects in gingivitis therapy. the potential benefits of local subgingival application of ha adjunctive to scaling and root planing (srp) were evaluated in this study. | 2004 | 15455740 |
| modified oligosaccharides as potential dental plaque control materials. | metabolic acids produced by oral pathogens demineralize tooth surfaces, leading to dental caries. glucosyltransferases are the key factor in this process. we synthesized various modified oligosaccharides and tested them for their inhibitory effects on glucosyltransferase activity. oligosaccharides were produced using a mixed-culture fermentation of lipomyces starkeyi and leuconostoc mesenteroides and then further modified as iron- and sulfate-oligosaccharides. iron- and sulfate-oligosaccharides ... | 2004 | 15458342 |
| characterization of a bacteriocin produced by prevotella nigrescens atcc 25261. | to characterize the antimicrobial activity produced by prevotella nigrescens atcc 25261, and to evaluate its safety on cultured gingival fibroblasts. | 2004 | 15482437 |
| the effect of periodontal treatment on the salivary bacterial load and early plaque formation. | the purpose of the present study was to investigate in periodontitis patients the relationship between the number of bacteria in the saliva and the amount of de novo plaque formation before and after treatment. | 2004 | 15491312 |
| subgingival microbiota of chronic periodontitis subjects from different geographic locations. | most clinical studies assume that the subgingival microbiota is similar from one geographic location to another. the purpose of the present investigation was to examine the composition of the subgingival microbiota in chronic periodontitis subjects from four countries. | 2004 | 15491316 |
| the distribution of periodontopathic bacteria among japanese children and their parents. | it is not well known how periodontopathic bacteria colonize in the oral cavity during childhood. the purpose of this study was to investigate the distribution of periodontopathic bacteria in oral cavities of children and their parents and the relationship between the bacterial findings and clinical parameters. | 2004 | 15491344 |
| prevotella intermedia lipopolysaccharide stimulates release of nitric oxide by inducing expression of inducible nitric oxide synthase. | the purpose of this study was to examine the effects of lipopolysaccharide from prevotella intermedia, a major cause of inflammatory periodontal disease, on the production of nitric oxide (no) and expression of inducible nitric oxide synthase (inos) in the murine macrophage cell line raw264.7. we also attempted to throw light on the signaling mechanisms involved in p. intermedia lipopolysaccharide-induced no production. | 2004 | 15491347 |
| periodontal pathogens in atheromatous plaques. a controlled clinical and laboratory trial. | a possible relationship between periodontitis and cardiovascular disease has been suggested. the aims of this controlled clinical study were: (i) to ascertain the presence of periodontal bacteria dna [actinobacillus actinomycetemcomitans, fusobacterium nucleatum, porphyromonas gingivalis, prevotella intermedia, tannerella forsythensis (formerly bacteroides forsythus)] in carotid atheromatous plaques and (ii) to assess the concomitant presence of the same periodontal bacteria dna, if any, in peri ... | 2004 | 15491349 |
| detection frequency of periodontitis-associated bacteria by polymerase chain reaction in subgingival and supragingival plaque of periodontitis and healthy subjects. | the aim of this study was to compare the detection frequencies of 25 bacterial species in subgingival and supragingival plaque of 18 untreated periodontitis subjects and 12 periodontally healthy subjects. genomic dna was extracted from subgingival and supragingival plaque samples, and bacterial detection was performed by polymerase chain reaction of the 16s rrna genes. fourteen bacteria showed no relationship with periodontitis, and 11 of these 14 species were frequently detected (> or =50%) in ... | 2004 | 15491463 |
| substance p enhances expression of lipopolysaccharide-induced inflammatory factors in dental pulp cells. | to examine how substance p (sp) is related with dental pulp inflammation, we examined the effects of sp on expression of genes for inflammatory factors in human dental pulp cell cultures. using reverse transcriptase-polymerase chain reaction, we found that prevotella intermedia lipopolysaccharide (lps) induced expression of sp and sp-receptor mrnas, and that somatostatin inhibited the lps-induced expression of sp mrna. we also found that sp enhanced lps-induced stimulation of nf-kappab binding a ... | 2004 | 15505507 |
| intra-familial distribution of nine putative periodontopathogens in dental plaque samples analyzed by pcr. | it is of great importance to understand the distribution of periodontopathogens within family members when considering the risk of periodontitis in children. the purpose of this study was to investigate the distribution of periodontopathogens among family members. we used the polymerase chain reaction method to test 4,8, and 7 probands with healthy gingiva, gingivitis, and periodontitis, respectively, and their 60 immediate family members. plaque samples were collected from all erupted teeth sit ... | 2004 | 15508747 |
| rate of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. | although microbiological studies have identified more than 400 bacterial species in periodontal pockets, only a limited number have been implicated as periodontal pathogens. the purpose of this study was to investigate the incidence of cultivable subgingival periodontopathogenic bacteria in chronic periodontitis. bacterial samples were collected with sterile paper points from the deepest periodontal pockets ((5 mm) of 203 patients: 92 males and 111 females, aged 35-55 years. the samples were cul ... | 2004 | 15508748 |
| [periodontitis and cardiovascular diseases--review of publications]. | nowadays periodontal diseases are treated as a one of a social diseases. the main consequences of them are: premature teeth loss and possibility of inducing, aggravating and modifying many systemic disorders, such as endo- and myocarditis, glomerulonephritis, iriditis, retinitis, rheumatic polyarthritis. the scientific data performed in the last 10 years indicate links between periodontitis and atheromatosis, coronary heart disease and acute coronary events, including myocardial infarction. in t ... | 2004 | 15515818 |
| colonization by porphyromonas gingivalis and prevotella intermedia from teeth to osseointegrated implant regions. | colonization by periodontopathic bacteria is a risk factor for peri-implantitis. the purpose of this study was to investigate the colonization by black-pigmented anaerobic bacteria that occurs between the time before fixture installation and 6 months after inserting superstructures in implant treatment in partial edentulous cases. dental plaque was serially collected from around the natural teeth and implants in 12 patients in whom a dental implant was indicated, and porphyromonas gingivalis and ... | 2004 | 15536858 |
| herpesviruses, the missing link between gingivitis and periodontitis? | herpesviruses appear to assume a major etiopathogenic role in various types of destructive periodontal disease. human cytomegalovirus (hcmv), epstein-barr virus (ebv) and hcmv-ebv co-infection are closely associated with disease-active periodontitis in juveniles and adults, with acute necrotizing ulcerative gingivitis in children, and with periodontal abscesses. in particular, hcmv reactivation in periodontitis lesions seems to be linked to advancing disease. hcmv infects periodontal monocytes/m ... | 2004 | 15553977 |
| single species biofilm-forming ability of root canal isolates on gutta-percha points. | the participation of bacterial biofilms in the over-filled gutta-percha points associated with refractory periapical periodontitis has recently been reported. this study investigated the initial biofilm-forming ability of root canal isolates (enterococcus faecalis, streptococcus sanguis, strep. intermedius, strep. pyogenes, staphylococcus aureus, fusobacterium nucleatum, propionibacterium acnes, porphyromonas gingivalis and prevotella intermedia) on gutta-percha points in vitro. each bacterial s ... | 2004 | 15560836 |
| oral flora in the age of molecular biology. | the present work describes the fundamental aspects of the molecular methods that are applied to oral microbiology: probes, pcr, multiple real time pcr, 16s rdna, and proteomics. likewise, it presents the results obtained by applying these methods to the study of the bacterial flora encountered inside the mouth. by identifying 16s rdna, up to 132 known species and 215 new, unknown phylotypes have been detected inside patients s periodontal pockets. we are currently using a commercial system based ... | 2004 | 15580127 |
| microbiological basis of oral infections and sensitivity to antibiotics. | because oral infections are common, the physician must understand the underlying etiology, pathogeny, and other variables that determine how these processes evolve in order to choose the most appropriate antibiotic drug. the special characteristics of the oral cavity determine the make-up of the microflora that lives there. different anaerobic species belonging to the peptostreptococcus, prevotella, fusobacterium, gemella, and porphyromonas genera are of particular interest, as are the aerobic s ... | 2004 | 15580129 |
| clinical and microbiological associations in chronic periodontitis. | chronic periodontitis is the most prevalent form of periodontal disease. the subgingival microflora has been associated with the onset and progression of the disease. the aim of this work was to establish associations and correlations between clinical indicators and subgingival plaque microbiological data in patients diagnosed with moderate or severe periodontitis. one hundred sites with a probing depth of > or = 5 mm were selected. plaque index (pi), gingival index (gi), bleeding on probing (bp ... | 2004 | 15584257 |
| the aetiology and treatment of oral halitosis: an update. | halitosis refers to the condition of offensive mouth odour. more than 90% of cases of halitosis originate from the oral cavity. the implicated bacteria (fusobacterium nucleatum, prevotella intermedia, and tannerella forsythensis) are located in stagnant areas in the oral cavity, such as the dorsal surface of tongue, periodontal pockets, and interproximal areas. these bacteria proteolyse the amino acids releasing volatile sulphur compounds. the management of halitosis involves determining and eli ... | 2004 | 15591601 |
| antibiotic resistance profile of the subgingival microbiota following systemic or local tetracycline therapy. | tetracyclines have been extensively used as adjunctives to conventional periodontal therapy. emergence of resistant strains, however, has been reported. this study evaluated longitudinally the tetracycline resistance patterns of the subgingival microbiota of periodontitis subjects treated with systemic or local tetracycline therapy+scaling and root planing (srp). | 2004 | 15142209 |
| bacterial and viral dna in periodontal disease: a study using multiplex pcr. | recent studies have suggested an association between periodontal disease and the presence of herpesviruses, in particular: epstein-barr virus (ebv) and human cytomegalovirus (cmv) (contreras et al., 1999--contreras et al., 2000--slots et al., 2000--ting et al., 2000). in the work reported in this paper, we use a multiplex polymerase chain reaction (pcr) to compare the presence of herpesviruses and putative bacterial pathogens in patients with periodontal disease and in healthy individuals. direc ... | 2004 | 15164623 |
| the bacteriology of the supragingival plaque of child dental patients in kuwait. | to investigate the bacterial flora of the gingival plaques of pediatric dental patients in order to establish baseline data for future studies on the complex microbiota of the oral cavity of this group of child dental patients. | 2004 | 15181322 |
| purification and characterization of a hemoglobin-binding outer membrane protein of prevotella intermedia. | an outer membrane hemoglobin-binding protein of prevotella intermedia was identified and purified in the present study. hemoglobin-binding protein was purified via a series of column chromatographic methods. the molecular mass of the purified protein, which was approximately 60 kda, was determined by sds-page. hemoglobin binding of the protein was examined by western blot and dot blot assays. hemoglobin-binding activity was ph dependent; the strongest binding activity occurred at ph 5.0. globin ... | 2004 | 15183882 |
| herpesviral-bacterial interrelationships in aggressive periodontitis. | recent findings have begun to provide a basis for a causal link between herpesviruses and aggressive periodontitis. one theory is that herpesviruses cooperate with specific bacteria in the etiopathogenesis of the disease. this study examined whether the presence of herpesviruses [human cytomegalovirus (hcmv), epstein-barr virus (ebv) type 1, herpes simplex virus (hsv) type 1 and 2] is associated with the presence of putative pathogenic bacteria (porphyromonas gingivalis, prevotella intermedia, t ... | 2004 | 15206912 |
| genetic analysis of an ambler class a extended-spectrum beta-lactamase from capnocytophaga ochracea. | a beta-lactamase gene (cfxa3, 966 bp) was isolated from a beta-lactam-resistant capnocytophaga ochracea clinical isolate and amplified using primers from the cfxa gene of bacteroides vulgatus. the mics of third-generation cephalosporins were much higher than those of the transconjugant escherichia coli strain. the deduced protein sequence, by comparison with cfxa2 of prevotella intermedia, had a y239d substitution and possessed the characteristics of a class a, group 2e beta-lactamase. | 2004 | 14766881 |
| gingival crevice microbiota from chinese patients with gingivitis or necrotizing ulcerative gingivitis. | the objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 chinese patients with gingivitis or necrotizing ulcerative gingivitis (nug). subjects were recruited in four dental clinics in eastern china. samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. the analyses showed that the fusiform taxa (fusobacter ... | 2004 | 14871191 |
| microbiological examination of infected dental root canals. | the aim of this study was to investigate the root canal microbiota of primary and secondary root-infected canals and the association of constituent species with specific endodontic signs and symptoms. | 2004 | 14871344 |
| subgingival microbiota of chilean patients with chronic periodontitis. | an association between race/ethnicity and the composition of the subgingival microbiota has been found in chronic periodontitis. a study was undertaken to determine the characteristics of the subgingival microbiota of chronic periodontitis in chileans residing in santiago. | 2004 | 15212354 |
| evaluation of the whole genome dna-dna hybridization technique to identify bacteria in histological sections of periradicular lesions. | whole genome dna-dna hybridization has been used to identify bacteria in periradicular lesions partly because there is no amplification of the bacteria, therefore, minor contaminants are not detected. there are, however, potential pitfalls with this technique, including inability to distinguish dead bacteria, cross-reactions of species within a genus, and inability to detect species present in low numbers because of loss of dna during extraction and purification. alternatively, inadequate extrac ... | 2004 | 15220650 |
| [necrotizing ulcerative periodontitis. diagnosis, treatment and follow-up--a case report]. | necrotizing ulcerative gingivitis (nug) and necrotizing ulcerative periodontitis (nup) share many clinical and etiologic characteristics. both forms are characterized by necrotic gingival tissues, bleeding and pain. malodor and malaise may be also present. nug is an infection limited to the gingiva whereas nup also involves the attachment apparatus. predisposing factors may include immune dysfunction, poor oral hygiene, cigarette smoking and emotional stress. nug and nup might possibly be differ ... | 2004 | 15250177 |
| [genetic and microbiologic tests in periodontal disease]. | the presence of genetic polymorphisms il-1a and il-1b was demonstrated to be associated to an increased clinical severity of periodontitis. the aim of our study was to verify the existence of a relationship between such genetic polymorphisms and pathogenic variations of actinobacillus actinomycetemcomitans (aa), prevotella intermedia (pi) and porphyromonans gingivalis (pg), in patients with periodontitis before and after active therapy. | 2004 | 15266289 |
| the microbial outcome observed with polymerase chain reaction in subjects with recurrent periodontal disease following local treatment with 25% metronidazole gel. | the aim of this study was to evaluate the microbial outcome in patients with recurrent periodontal disease following treatment with 25% metronidazole gel using the polymerase chain reaction (pcr). twenty subjects in a maintenance care program but with recurrent periodontal disease participated. three months after scaling and root planing a total of 40 sites, 2 in each patient, with pocket probing depth of > or = 5 mm were selected. one site randomly selected was treated with 25% metronidazole ge ... | 2004 | 15272511 |
| implant therapy following liver transplantation: clinical and microbiological results after 10 years. | the number of immunocompromised patients is increasing as a consequence of successful organ transplantation. placing dental implants in these patients has been questioned because of their increased risk for infections. the 10-year follow-up data of a 71-year-old liver transplant recipient with long-term immunosuppressive therapy is reported. six months after liver transplantation, two interforaminal implants were inserted in the edentulous mandible, and an overdenture using non-rigid telescopic ... | 2004 | 15295960 |
| microbiological shifts in intra- and extraoral habitats following mechanical periodontal therapy. | the aim of the present study was to analyze the intra- and extraoral colonization dynamics of periodontal pathogens following supra- and subgingival debridement. | 2004 | 15312101 |
| induction of vascular endothelial growth factor expression in human pulp fibroblasts stimulated with black-pigmented bacteroides. | to investigate the effect of black-pigmented bacteroides on the expression of vascular endothelial growth factor (vegf) gene in human pulp fibroblasts. | 2004 | 15317561 |
| initial antimicrobial effect of controlled-release doxycycline in subgingival sites. | to determine the ability of a 10% doxycycline hyclate controlled-release polymer (atridox) to suppress periodontopathic bacteria when placed subgingivally following scaling and root planing (sc/rp). | 2004 | 15324352 |
| quantitative detection of periodontopathogens by real-time pcr. | specific bacteria are believed to play an important role in chronic periodontitis, yet the significance of their relative numbers in initiation and progress of the disease is still unclear. we report here the development of a sensitive, quantitative pcr technique for enumerating actinobacillus actinomycetemcomitans (aa), porphyromonas gingivalis (pg), prevotella intermedia (pi), dialister pneumosintes (dp) and micromonas micros (mm) as well as total eubacteria in subgingival plaque samples from ... | 2004 | 15325758 |
| microbiological profile of early onset/aggressive periodontitis patients. | the objectives of this study were to characterize the bacterial profile and to seek possible bacterial associations in the subgingival microbiota of early onset periodontitis/aggressive periodontitis patients by using two different techniques, culture and immunofluorescence. | 2004 | 15327644 |
| detection of dna from periodontal pathogenic bacteria in biofilm obtained from waterlines in dental units. | direct person-to-person transmission of periodontal bacteria through saliva has recently been widely reported and dental units have been demonstrated to retract saliva from patients under treatment and to release it into the mouths of subjects undergoing the next operation. in this study the presence of a group of periodontal pathogenic bacteria inside waterlines in dental units was investigated using polymerase chain reaction (pcr) based methods. briefly, 18 dental units of three different manu ... | 2004 | 15646054 |
| pi30 dna probe may be useful for the identification of prevotella intermedia at the species or strain level. | recently, we introduced a new method for the rapid screening of bacterial species-or subspecies-specific dna probes, named the "inverted dot blot hybridization screening method." this method has subsequently been then applied to develop species-or strain-specific dna probes for prevotella intermedia and prevotella nigrescens. in a previous study, the inverted dot blot hybridization data showed that a probe, pi30, was specific for p. intermedia. in this study, the dna probe pi30 was evaluated by ... | 2004 | 15611609 |
| susceptibility of some oral microorganisms to chlorhexidine and paramonochlorophenol. | since the use of antimicrobial agents is required in endodontic therapies, this study aimed at determining the minimum inhibitory concentrations (mics) of chlorhexidine digluconate and paramonochlorophenol (pmc) against microorganisms commonly found in endodontic infections. both agents were tested by agar dilution tests against pseudomonas aeruginosa, staphylococcus aureus, enterococcus faecalis, escherichia coli, candida albicans, prevotella intermedia, porphyromonas gingivalis, porphyromonas ... | 2004 | 15619879 |
| attachment of oral gram-negative anaerobic rods to a smooth titanium surface: an electron microscopy study. | attachment of bacteria to titanium may differ not only between bacterial species but also between strains within a species. the aim of the present in vitro study was to examine differences in bacterial attachment using 4 gram-negative anaerobic species of bacteria that are considered potential periodontal pathogens. | 2004 | 15623054 |
| the role of anaerobic bacteria in tonsillitis. | this review summarizes the information that supports the potential importance of anaerobic bacteria in tonsillitis. some anaerobic bacteria possess interfering capability with group a beta-hemolytic streptococci (gabhs) and other pathogens. the possible role of anaerobes in the acute inflammatory process in the tonsils is supported by several observations: anaerobes have been isolated from the cores of tonsils of patients with recurrent gabhs and non-gabhs tonsillitis (nst); the recovery of anae ... | 2005 | 15627441 |
| differences in the subgingival microbiota of swedish and usa subjects who were periodontally healthy or exhibited minimal periodontal disease. | previous studies have shown differences in the mean proportions of subgingival species in samples from periodontitis subjects in different countries, which may relate to differences in diet, genetics, disease susceptibility and manifestation. the purpose of the present investigation was to determine whether there were differences in the subgingival microbiotas of swedish and american subjects who exhibited periodontal health or minimal periodontal disease. | 2005 | 15642056 |
| streptococci dominate the diverse flora within buccal cells. | previously, we reported that intracellular actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and tannerella forsythensis were present within buccal epithelial cells from human subjects, as lesser components of a polymicrobial flora. in this study, we further characterized that intracellular flora by using the same double-labeling techniques to identify fusobacterium nucleatum, prevotella intermedia, oral campylobacter species, eikenella corrodens, treponema denticola, gemella haemo ... | 2005 | 16304448 |
| injectable polysaccharide microcapsules for prolonged release of minocycline for the treatment of periodontitis. | injectable polysaccharide microcapsules holding minocycline were fabricated from alginate and chitosan for the treatment of periodontitis. the microcapsules were examined for the release and degradation of minocycline, as well as antimicrobial activity. the microcapsules were biodegradable and released minocycline between 10 and 1000 microg ml(-1), which was higher than the usual therapeutic concentration (1-5 microg ml(-1)), for up to 7 days. these microcapsules showed a statistically significa ... | 2005 | 16314967 |
| [necrotizing periodontal disease: a manifestation of systemic disorders]. | necrotizing periodontal disease (npd) is an infection characterized by gingival necrosis presenting as "punched-out" papillae, with gingival bleeding, and pain. prevotella intermedia and spirochetes have been associated with the gingival lesions. predisposing factors may include emotional stress, immunosuppression, especially secondary to human immunodeficiency virus (hiv) infection, cigarette smoking, poor diet and pre-existing gingivitis. during the last few years, diagnosis of npd has became ... | 2005 | 16324485 |
| [bacterial spectra and antibiotics in odontogenic infections. renaissance of the penicillins?]. | the role played by odontogenic infection in dental, oral, and maxillofacial surgery is not to be underestimated even at the present time. an extensive, standardized, prospective study was performed with the intention of verifying the bacterial spectrum of odontogenic infections to evaluate antibiotic sensitivity. | 2005 | 16261393 |
| clinical and microbiological characterization of periodontal abscesses. | the knowledge of clinical features, microbial composition and susceptibility to antimicrobials of periodontal abscesses has recently improved. this descriptive clinical and microbiological study provides more information on the characteristics of periodontal abscesses. | 2005 | 16268997 |
| [experience in medical ozone use for root canal treatment]. | the results of clinical and laboratory assessment of effectiveness of psr-diagnosis are presented. the high efficacy of ozone therapy is revealed. the findings demonstrate the reduction of number in the micro-organisms in root canal: actinobacillus actinomycetemcomitans from 31.25% to 10.21%; bacteriodes forsythus from 68.75% to 15.50%; treponema denticola from 37.5% to 11.4%; porphyromonas gingivalis from 56.25% to 45%; prevotella intermedia from 16% to 0%. | 2005 | 16353031 |
| antimicrobial effect in vitro of chlorhexidine and calcium hydroxide impregnated gutta-percha points. | the aim of this study was to evaluate the antimicrobial activity of points containing antiseptics used for temporary obturation in endodontics. points containing calcium hydroxide (roeko and hygienic), and chlorhexidine (roeko) were tested and gutta-percha points served as control (meta dental corp.). the following microorganisms were studied: streptococcus mutans (sm), enterococcus faecalis (ef), staphylococcus aureus (sta), candida albicans (ca), fusobacterium nucleatum (fn) atcc 25586, porphy ... | 2005 | 16673792 |
| serum antibodies to oral anaerobic bacteria in patients with rheumatoid arthritis. | this study was conducted to determine the component that causes the disease in rheumatoid arthritis (ra), which shows great resemblance to periodontitis in a pathologic context. | 2005 | 16369381 |
| detection of tannerella forsythia and/or prevotella intermedia might be useful for microbial predictive markers for the outcome of initial periodontal treatment in koreans. | a proportion of diseased sites in periodontal disease do not respond to the initial treatment, which might be due in part to the presence of specific microbial pathogens. the aim of this study was to clarify the value of microbial screening for predicting the outcome of periodontal treatment in koreans using a polymerase chain reaction (pcr). this study enrolled 32 adults with periodontal disease. microbial and clinical examinations were performed at the baseline and after the initial treatment ... | 2005 | 15665448 |
| periodontal infections and pre-term low birth weight: a case-control study. | pre-term delivery of low-birth-weight infants [pre-term low birth weight (plbw)] remains a significant public health issue and a major cause of neonatal death and long-term health problems. there is a growing consensus that infections remote from fetal-placental unit may influence plbw infants. recent studies have suggested that maternal periodontal disease may be an independent risk factor for plbw. the purpose of the present study was to evaluate the possible link between periodontal infection ... | 2005 | 15691348 |
| clinical and microbiological effect of scaling and root planing in smoker and non-smoker chronic and aggressive periodontitis patients. | to compare the effects of scaling and root planing (srp) on clinical and microbiological parameters at selected sites in smoker and non-smoker chronic and generalized aggressive periodontitis patients. | 2005 | 15691352 |
| antimicrobial actions of benzimidazoles against oral streptococci. | benzimidazoles, such as lansoprazole and omeprazole, are used extensively as proton-pump inhibitors to control stomach acid secretion and also have antimicrobial actions against helicobacter pylori. our objective was to determine whether they are potentially useful antimicrobials against oral bacteria. | 2005 | 15720569 |
| antimicrobial resistance and beta-lactamase production of clinical isolates of prevotella and porphyromonas species. | this study determined the beta-lactamase production and the antimicrobial resistance of 72 prevotella species and 48 porphyromonas species isolated from different clinical specimens. | 2005 | 15722627 |
| combined effects of nd:yag laser irradiation with local antibiotic application into periodontal pockets. | the purpose of the present study was to assess the effects of nd:yag laser irradiation into periodontal pockets with or without the combination of local antibiotic application on clinical parameters and microbiological prevalence. sixteen patients, each of whom had 4 or more sites with probing depth e x 4mm were included in this study. they were monitored clinically and microbiologically at baseline, 1 and 3 months after the treatment. subgingival plaque samples were taken from periodontally inv ... | 2005 | 15736890 |
| using photodynamic therapy to treat peri-implantitis. interview. | 2005 | 15747830 | |
| lethal photosensitization of oral pathogens via red-filtered halogen lamp. | objective of investigation: the ability of laser irradiation in the presence of photosensitizing agent to induce lethal effect on oral bacteria is well documented. we designed an in-vitro experiment to achieve phototoxic results on two common oral pathogens, using a high intensity, red filtered halogen lamp. our goal was to determine the minimum duration of light exposure and drug dilution to achieve at least 50% reduction in bacteria counts. | 2005 | 15752111 |
| [pharmacokinetic/pharmacodynamic analysis of antibiotic therapy in dentistry and stomatology]. | this study evaluates the efficacy of various antimicrobial treatments for orofacial infections on the basis of pharmacokinetic/pharmacodynamic (pk/pd) criteria. | 2005 | 15757581 |
| phototargeting oral black-pigmented bacteria. | we have found that broadband light (380 to 520 nm) rapidly and selectively kills oral black-pigmented bacteria (bpb) in pure cultures and in dental plaque samples obtained from human subjects with chronic periodontitis. we hypothesize that this killing effect is a result of light excitation of their endogenous porphyrins. cultures of prevotella intermedia and p. nigrescens were killed by 4.2 j/cm2, whereas p. melaninogenica required 21 j/cm2. exposure to light with a fluence of 42 j/cm2 produced ... | 2005 | 15793117 |
| dna probe identification of bacteria colonizing internal surfaces of the implant-abutment interface: a preliminary study. | currently, there is limited knowledge concerning the specific genus and species of bacteria that may colonize internal surfaces of the implant-abutment interface (iai) of two-stage dental implants. the purpose of this study was to use dna probe analysis to identify those periodontopathic bacteria that may inhabit the internal surfaces and healing abutment screw-threads of the iai of dental implants in situ. | 2005 | 15830645 |
| detection of heat shock proteins but not superantigen by isolated oral bacteria from patients with behcet's disease. | we isolated bacteria from periodontal sites and mixed saliva in eight patients with behcet's disease and surveyed them to determine whether they produced heat shock proteins (hsps) and superantigen. cultivable bacterial compositions from periodontal sites and saliva were examined by anaerobic culture using blood agar plates. gram-negative anaerobic rods such as prevotella intermedia, fusobacterium nucleatum, and capnocytophaga species were predominant in the isolates from the subgingival plaque ... | 2005 | 15836518 |
| treponema medium glycoconjugate inhibits activation of human gingival fibroblasts stimulated with phenol-water extracts of periodontopathic bacteria. | oral treponemes are well-known as causative agents of periodontal diseases; however, the details have not been fully clarified. here, we examined the effects of treponema medium glycoconjugate on the activation of human gingival fibroblasts using phenol-water extracts from porphyromonas gingivalis, prevotella intermedia, fusobacterium nucleatum subsp. nucleatum, and actinobacillus actinomycetemcomitans. the phenol-water extracts activated human gingival fibroblasts to mediate il-8 production, as ... | 2005 | 15840783 |