Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| rescue of infectious virus from nonproducer rous cells by chick cellular dna. | the rescue of infectious virus from nonproducer bh rsv(-) cells by chick cellular dna was attempted in order to investigate the functional state of endogenous and exogenous retroviral genes integrated within the cellular dna. no infectious virus was rescued by transfection with dnas of chick helper factor (chf)-negative chick embryo cells (cec), chf-positive cec or uninfected cec producing endogenous rous associated virus (rav-0). on the other hand, infectious rous viruses with the phenotype of ... | 1989 | 2558502 |
| alkaline phosphatase fusions to the respiratory syncytial virus f protein as an approach to analyze its membrane topology. | manoil and beckwith (1985) have constructed a transposon, tnphoa, that permits the generation of hybrid proteins composed of alkaline phosphatase (ap) lacking its signal peptide fused to amino-terminal sequences of other proteins. this transposon has been used to localize export signals and analyze membrane topology of bacterial proteins. we have applied this approach to the membrane fusion protein (f) of respiratory syncytial virus (rsv). the transposon tnphoa and a plasmid directing bacterial ... | 1989 | 2558867 |
| potential usefulness of a cultured glioma cell line induced by rous sarcoma virus in b10.a mouse as an immunotherapy model. | a cultured glioma cell line, sr-b10.a, which was derived from a brain tumor induced in an adult female b10.a mouse by rous sarcoma virus (rsv), has been established. the morphological appearance of the tumor produced by s.c. inoculating sr-b10.a cells was analogous to an astrocytoma of human glioma. glial fibrillary acidic protein as well as s-100 protein was positive in these sr-b10.a tumor cells. a population doubling time of the cultured cells was 18.5 hours. chromosomal analysis revealed a d ... | 1989 | 2559217 |
| cellular immunity in chicks expressing fibrosarcomatous liver tumor following embryo infection with subgroup a rous sarcoma virus. | bryan standard strain of rous sarcoma virus (bs-rsv) of subgroup a was inoculated into heavy and light breeds of chicken embryos via chorioallantoic membrane (cam) to ascertain cell-mediated immune response, as measured by a leukocyte migration inhibition (lmi) test. chicks hatched from eggs with pock-positive cams were more likely to develop liver tumors than those hatched from eggs with pock-negative cams. chicks that developed tumors usually had a positive cell-mediated immune response, and t ... | 1989 | 2559698 |
| expression of a prokaryotic gene in cultured lung endothelial cells after lipofection with a plasmid vector. | transfection of cultured cells with functioning foreign genes can be a powerful tool for delineating mechanisms controlling gene expression and for manipulating cellular synthetic machinery. we transfected cultured bovine pulmonary artery endothelial cells with a plasmid (prsvcat) containing the prokaryotic gene, chloramphenicol acetyltransferase (cat), driven by a rous sarcoma virus (rsv) promoter. transfection was accomplished by binding the plasmid dna to specially synthesized cationic liposo ... | 1989 | 2559763 |
| insulin enhances glucocorticoid induction of gene expression in a sequence specific manner. | glucocorticoid receptor (gr) induces transcriptional activation of specific genes by binding to cis-acting enhancer sequences termed glucocorticoid responsive elements (gres). insulin regulates gene transcription part of which is mediated sequences dominated by gres. by transient expression in htc cells of fusion gene consist mmtv 5' flanking sequences containing the gres on the thymidine kinase promoter (tk) and the coding region of the chloramphenicol acetyl transferase gene (cat), we demonstr ... | 1989 | 2560516 |
| a variant schmidt-ruppin strain of rous sarcoma virus with increased affinity for mammalian cells. | sr-rsv-d(h), a variant virus with extremely high tropism for mammalian cells, was isolated by passage of the schmidt-ruppin strain of rous sarcoma virus of subgroup d (sr-rsv-d) through hamster cells. this variant virus has acquired an altered envelope glycoprotein, encoded by the env gene, that has high affinity for receptors on the surface of mammalian cells. the variant virus transforms rat cells at about 100 times the efficiency of the parental virus, sr-rsv-d(s), as assayed by focus formati ... | 1989 | 2560773 |
| augmentation of anti-tumor immunity in low-responder mice by various biological response modifiers: analysis of effector mechanism. | in order to elucidate the role of biological response modifiers (brms) in anti-tumor immunotherapy, we examined their effect on the induction of anti-tumor immunity in low-responder mice which hardly exhibit anti-tumor resistance against syngeneic rous sarcoma virus (rsv)-induced tumors, such as b10 or b10.br mice. the anti-tumor immunity induction in the low-responder mice was 0% on immunization with mitomycin c-treated syngeneic tumor cells alone. however, if brms were used as an adjuvant, bcg ... | 1989 | 2560774 |
| analysis of b-g and immune response genes in the iowa state university s1 chicken line by hybridization of sperm deoxyribonucleic acid with a major histocompatibility complex class ii probe. | sperm dna was isolated from chickens of the iowa state university s1 line. birds were from sublines selected for b-g antigen, humoral immune response to glutamic acid-alanine-tyrosine (irgat), and response to rous sarcoma virus-induced (rsv) tumors. the dna was digested with restriction enzymes and subjected to southern blot analysis with a dna probe specific for a class ii gene of the chicken major histocompatibility complex (mhc). restriction fragment length polymorphisms (rflp) associated wit ... | 1989 | 2565033 |
| occurrence and aetiology of acute respiratory diseases: results of a longterm surveillance programme. | totals of 58,661,000 acute respiratory disease (ard) cases, 1,376,651 bronchitis and pneumonia complications, and 93,042 deaths from influenza, bronchitis, pneumonia or chronic pulmonary affection were notified during 11 years of ard surveillance from 1975 to 1986. all ard seasons started with the first phase in september-december; this increase in morbidity was caused chiefly by adenoviruses, parainfluenza viruses, rhinoviruses and m. pneumoniae. second wave of ard morbidity occurring in januar ... | 1989 | 2565674 |
| synthesis of respiratory syncytial virus rna in cell-free extracts. | a cell-free system has been used to synthesize respiratory syncytial virus (rsv) rna in vitro. polyadenylated species representing all size classes of rsv mrnas were labelled. some of the labelled rna was seen in a cscl gradient at the density characteristic of negative-strand viral nucleocapsids. experiments using a thiotriphos-phorylated nucleotide indicate that rna chains are initiated de novo in the cell-free system. | 1989 | 2567342 |
| increase in ribosomal protein s6 phosphorylation is due to v-erbb-transforming activity and not to v-erba mitogenic activity in avian erythroblastosis virus-infected chicken embryo fibroblasts. | avian erythroblastosis virus (aev-es4), a transforming avian retrovirus, transforms chicken embryo fibroblasts (cefs) in culture and induces the maintenance of ribosomal protein s6 phosphorylation in the absence of serum. this effect is less pronounced after aev-es4 transformation than after transformation by rous sarcoma virus (pr-rsv a). however, our results indicate that the two viruses induce an activation of the same s6 phosphokinase, as evidenced by the identity of s6 phosphopeptides and p ... | 1989 | 2567979 |
| effects of norakin on respiratory syncytial virus in tissue culture and in mice. | norakin at 1 microgram/ml inhibits the reproduction of respiratory syncytial virus (rsv) in vero cells to 50% and at 5 micrograms/ml to 90%. the development of lung lesions in rsv-infected balb/c mice was suppressed by 70% when the animals were treated with two doses (25 mg/kg each) of norakin, 30 min before and 4 hr post infection. (p.i.), respectively. | 1989 | 2569812 |
| serum antibodies against respiratory tract viruses: a prospective three-year follow-up from birth. | acute otitis media (aom) has been epidemiologically related to viral respiratory tract infections, and viral antigens have also been detected in middle ear secretion in some aom episodes. successive serum samples from children followed prospectively for three years from birth were analysed for igg antibodies against respiratory syncytial virus (rsv), adenoviruses and influenza a virus. values from serum antibody activity gradually decreased during the first six months of life, followed by a grad ... | 1989 | 2584849 |
| respiratory syncytial virus: a nursing perspective. | respiratory syncytial virus (rsv), a common winter illness, is most devastating in infants with underlying pulmonary and cardiovascular disease. ribavirin aerosol is an effective treatment when used with appropriate nursing management. | 1989 | 2587088 |
| detection of multiple viral agents in nasopharyngeal specimens yielding respiratory syncytial virus (rsv). an assessment of diagnostic strategy and clinical significance. | a retrospective study of 6 years (1981-1987) experience with clinical specimens of pediatric patients submitted for identification of respiratory viruses was undertaken to determine the prevalence of multiple viral isolates and to assess the impact of dual infections on severity of clinical disease. respiratory syncytial virus (rsv), the most frequently identified agent, was detected in cell culture and/or by immunofluorescence (if) in 666 of 2,415 specimens examined. a second virus was isolated ... | 1989 | 2591168 |
| hla class i antigens in severe rsv bronchiolitis. | cytotoxic t-lymphocytes (ctl) recognize virus-infected cells in association with hla class i antigens. there is strong evidence of the importance of ctl in respiratory syncytial virus (rsv) infections. we looked for but were unable to demonstrate an association between particular hla class i antigens and severe rsv bronchiolitis in infants. | 1989 | 2595727 |
| [severe respiratory syncytial virus infections. study of 87 infants hospitalized in an epidemic]. | during a winter epidemic, 87 infants were admitted to necker-enfants-malades hospital with a severe respiratory syncitial virus (rsv) infection. these infants fell into two groups: 37 infants without any medical history and 50 showing an underlying pathology (immune deficiencies, heart disease, cns disorders, digestive malformations, allergic manifestations). of the 37 infants with no medical history, most were below the age of 6 months and the rsv infection was manifested clinically by bronchio ... | 1989 | 2604509 |
| antiviral activities and the mechanism of 9-beta-d-ribofuranosyl-6-alkylthiopurines on several rna viruses from animals. | a series of 9-beta-d-ribofuranosyl-6-alkylthiopurines (6-alkyl ti) were found to inhibit in vitro replication of infectious hematopoietic necrosis virus (ihnv), human influenza virus (ifv) and respiratory syncytial virus (rsv) with ic50 values of about 0.06 microgram/ml, 0.7-1.5 micrograms/ml and 1-3 micrograms/ml, respectively. viral rna synthesis in infected cells in the presence of actinomycin d was inhibited by treatment with the compounds dose-dependently. it was also found that the decreas ... | 1989 | 2608474 |
| the 1a protein of respiratory syncytial virus is an integral membrane protein present as multiple, structurally distinct species. | the respiratory syncytial virus (rsv) 1a protein was previously identified as a 7.5-kilodalton (kda) nonglycosylated species that, on the basis of its predicted sequence determined from the sequence of its mrna, contains a hydrophobic central domain that was suggestive of membrane interaction. here, four major, structurally distinct intracellular species of the 1a protein were identified in cells infected by rsv or by a recombinant vaccinia virus expressing the 1a gene. the four species of 1a we ... | 1989 | 2649692 |
| comparison of the anti-respiratory syncytial virus activity and toxicity of papaverine hydrochloride and pyrazofurin in vitro and in vivo. | based on reports describing their broad antiviral activity, the toxicity and antiviral efficacy of papaverine hydrochloride and pyrazofurin against respiratory syncytial virus (rsv) infection were tested in vitro in tissue culture cells and in vivo in cotton rats. papaverine inhibited rsv replication in vitro; however, the median minimal toxic dose-median minimal inhibitory concentration ratios (mtd50:mic50) in vitro and in vivo for papaverine were less than 4. further work with this compound wa ... | 1989 | 2653219 |
| use of high-speed rolling to detect respiratory syncytial virus in cell culture. | we examined the effect of motion on respiratory syncytial virus (rsv) growth in cell culture. infected cultures were incubated stationary, rolling, or on an orbital shaker. enzyme immunoassay (eia) results for cultures infected with high concentrations of a laboratory strain of rsv were similar for all incubation conditions. however, cultures infected with low concentrations of virus and rolled at 96 rpm had a significantly greater mean eia optical density (1.78 +/- 0.22) than cultures rolled at ... | 1989 | 2654185 |
| respiratory syncytial virus alters electrophysiologic properties in cotton rat airway epithelium. | the effect of respiratory syncytial virus (rsv) infection on the electrophysiologic properties of the airway epithelium was studied in tracheas obtained from cotton rats, after in vivo exposure to the virus. rsv infection was documented by tissue culture infectivity and immunofluorescent antibody techniques. light microscopic studies of the tracheas 72 hours after exposure to rsv revealed normal epithelial morphology. rsv infection produced a 28% decrease in short-circuit current (isc) and an al ... | 1989 | 2654848 |
| mechanism of lung injury in cotton rats immunized with formalin-inactivated respiratory syncytial virus. | respiratory syncytial virus (rsv) seronegative cotton rats were immunized intramuscularly at four and eight weeks of age with either formalin-inactivated, alum-precipitated rsv grown in hep-2 cell tissue cultures or virus-free hep-2 cell tissue cultures similarly prepared. sham-immunized animals served as controls. at 12 weeks of age, all animals were challenged with 6 x 10(5) plaque forming units of live rsv via the intranasal route. animals were killed at predetermined days and evaluated for r ... | 1989 | 2655343 |
| a sensitive technique to monitor gene transfer and expression in bone marrow stem cells. | the polymerase chain reaction technique (pcr), a primer-mediated enzymatic amplification of specific target sequences, was used to monitor gene transfer into hematopoietic progenitor stem cells. a gene coding for human interleukin 1 alpha (il-1 alpha) was cotransfected with the rous sarcoma virus (rsv) cat plasmid into mouse bone marrow cells by electroporation. individual chloramphenicol (cam)-resistant bone marrow progenitor colonies (granulocyte-macrophage colony-forming units; cfu-gm) contai ... | 1989 | 2666145 |
| evaluation of the abbott testpack rsv enzyme immunoassay for detection of respiratory syncytial virus in nasopharyngeal swab specimens. | the abbott testpack rsv assay (abbott laboratories, north chicago, ill.), a rapid (20-min) enzyme immunoassay, was compared with culture and direct immunofluorescence (dfa) of nasopharyngeal cells for the detection of respiratory syncytial virus (rsv) in nasopharyngeal swab specimens. nasopharyngeal swab specimens, collected from 234 infants, were placed in viral transport medium. portions of specimen in transport medium were used for each test. of 234 specimens, 70 (30%) were culture positive, ... | 1989 | 2666434 |
| development of cell-mediated cytotoxic immunity to respiratory syncytial virus in human infants following naturally acquired infection. | with virus-infected autologous and allogenic mononuclear cells as specific targets, the development of cell-mediated cytotoxic reactivity to respiratory syncytial virus (rsv) was studied in peripheral blood lymphocytes (pbl) in groups of infants with acute rsv infection and in other control groups of subjects during a community outbreak of rsv infection. no rsv-specific cellular cytotoxicity was observed in cord blood lymphocytes and in other uninfected controls. the pbl of infants with acute rs ... | 1989 | 2666570 |
| antigenic characterization of respiratory syncytial virus associated with acute respiratory infections in uruguayan children from 1985 to 1987. | the occurrence of subgroup a and b strains of respiratory syncytial virus (rsv) was studied during three epidemic years, 1985 to 1987, in uruguay. a set of monoclonal antibodies was selected according to their reactivity with local rsv isolates and used for the typing of rsv directly in nasopharyngeal cells by indirect immunofluorescence. of 77 specimens, 69 could be typed as belonging to subgroup a or b, 5 could not be typed with the restricted set of monoclonal antibodies employed, and 3 react ... | 1989 | 2671013 |
| detection of respiratory syncytial virus in nasopharyngeal secretions by dna-rna hybridization. | we have developed an rna-cdna hybridization assay for the detection of respiratory syncytial virus (rsv) rna in nasopharyngeal samples. we chose to use as probe a cdna complementary to the nucleocapsid protein gene of rsv, integrated into the plasmid vector pbr322. the lower limit of sensitivity of the assay is 8.2 x 10(2) pfu of the long strain of rsv. in throat washes with added cell-free virus, the assay can detect 3.3 x 10(3) pfu of rsv. respiratory secretions were collected from a group of ... | 1989 | 2671029 |
| immunosuppression of the antibody response to respiratory syncytial virus (rsv) by pre-existing serum antibodies: partial prevention by topical infection of the respiratory tract with vaccinia virus-rsv recombinants. | immunization strategies to prevent respiratory syncytial virus (rsv) disease will involve immunization of infants less than 2 months of age who possess maternally derived rsv antibodies. vaccinia-rsv recombinant viruses are useful tools for defining parameters important in immunization against rsv and also are being considered as live virus vaccines for use in humans. previous studies demonstrated that passively acquired rsv antibodies can suppress the immune response and the protective efficacy ... | 1989 | 2671259 |
| rapid detection of respiratory syncytial virus in nasopharyngeal secretions by immunofluorescence and elisa does not justify discontinuation of virus isolation. | direct fluorescent antibody assay (dfa) using monoclonal antibody and enzyme-linked immunosorbent assay (elisa) for rapid detection of respiratory syncytial virus (rsv) in nasopharyngeal secretions (nps) were compared with conventional virus isolation and identification procedures in cell cultures. when 134 nps were examined from infants and young children with acute respiratory tract infection, 42 (31%) were culture-positive for rsv and 31 of these were detected by the appearance of a typical c ... | 1989 | 2674634 |
| viral etiology in acute lower respiratory infections in children from a closed community. | the aim of this study was to determine the viral agents associated with acute lower respiratory infections (alri) in young children. during a 2-yr period, 204 nasopharyngeal aspirates (npa) from children under 4 yr of age living in an orphanage and exhibiting febrile alri were studied by both indirect immunofluorescence (iif) and isolation in four cell lines. npa cell smears as well as tissue culture cells exhibiting cytopathic effect (cpe) or hemadsorption were stained by iif for respiratory sy ... | 1989 | 2675703 |
| protection of cotton rats against human respiratory syncytial virus by vaccination with a novel chimeric fg glycoprotein. | the cotton rat model of experimental human respiratory syncytial virus (rsv) infection was used to study the efficacy of fg, a novel chimeric glycoprotein which was expressed in insect cells using a baculovirus vector. fg contained the extracellular regions of the f (fusion) and g (attachment) glycoproteins of rsv. vaccination with fg resulted in induction of neutralizing antibody and was correlated with protection of lung tissue from rsv challenge against both serogroup a and b virus strains. b ... | 1989 | 2677234 |
| respiratory syncytial virus-induced acute lung injury in adult patients with bone marrow transplants: a clinical approach and review of the literature. | acute lung injury induced by respiratory syncytial virus (rsv) is a major cause of morbidity and mortality in patients who have undergone bone marrow transplantation. twenty-nine of the 74 patients who received bone marrow transplants at the university of minnesota during a 1-year period developed evidence of acute lung injury, and rsv was identified as the cause in 8. we discuss the clinical course of these 8 patients and offer a clinical approach to rsv infection occurring after bone marrow tr ... | 1989 | 2677595 |
| [outbreak of respiratory syncytial virus in a neonatal unit]. | described is an outbreak of nosocomial rsv infection, following the admission of a 20-day-old and 11-day-old newborn with lower respiratory infections, diagnosed as being caused by rsv through detection of viral antigens by direct fat (fluorescent antibody test) of nasopharyngeal samples. later on, 5 of the other 20 newborn in patients in the same care unit also showed positive results in direct fat, for rsv. the clinical manifestations of these patients were mild even in those cases with underl ... | 1989 | 2679277 |
| eukaryotic promoters drive gene expression in escherichia coli. | eight eukaryotic promoters have been tested for their activity in vivo in escherichia coli. the rat beta-actin, rat amylase, rat chymotrypsin b, mouse metallothionein i, rat insulin i, human insulin, rous sarcoma virus long terminal repeat (rsv ltr) and hepatitis b viral precore promoter activities were measured by using the bacterial chloramphenicol acetyltransferase coding sequences as the reporter function and by primer extension rna analysis. all eight promoter-chloramphenicol acetyltransfer ... | 1989 | 2681182 |
| immunization of cotton rats with the fusion (f) and large (g) glycoproteins of respiratory syncytial virus (rsv) protects against rsv challenge without potentiating rsv disease. | a formalin-inactivated respiratory syncytial virus (rsv) vaccine tested 22 years ago failed to protect infant vaccinees against rsv infection or disease. instead, lower respiratory tract disease was enhanced during subsequent infection by rsv. enhancement of pulmonary pathology is also observed when cotton rats are immunized with formalin-inactivated rsv and subsequently infected with this virus. a major question that must be addressed for each new paramyxovirus vaccine is whether the immunogen ... | 1989 | 2692334 |
| rapid detection of respiratory syncytial virus by a biotin-enhanced immunoassay: test performance by laboratory technologists and housestaff. | a biotin-enhanced enzyme immunoassay (eia) for respiratory syncytial virus (rsv) antigen detection (testpack rsv) was prospectively compared with virus isolation in cell culture and immunofluorescence. of 156 nasopharyngeal swab specimens from infants with respiratory symptoms, 81 (52%) yielded rsv in culture. compared with culture the sensitivity of the eia was 95% and specificity was 92%; the specificity increased to 97% with a blocking assay. compared with immunofluorescence the sensitivity o ... | 1989 | 2696927 |
| [effect of the environment on the development of respiratory allergies]. | allergy reactions may be regarded as resulting from exposure to allergens of subjects who are genetically predisposed to synthesize ige. the presence of allergens in the environment is necessary to acquire sensitization. with some allergens, sensitization seems to develop very rapidly. the introduction or presence in the environment of certain allergens, such as house dust mites, may increase the prevalence of asthma. despite conflicting reports, in atopic subjects exposure to pet animals also i ... | 1989 | 2699066 |
| serum immunoglobulin g antibody subclass response to respiratory syncytial virus f and g glycoproteins after first, second, and third infections. | serum samples from 31 children who experienced two or three infections with respiratory syncytial virus (rsv) in the first four years of life were tested in an enzyme-linked immunosorbent assay to examine the immunoglobulin g (igg) subclass responses to the rsv f and g surface glycoproteins associated with primary infection and reinfection. we sought to determine whether the greater degree of glycosylation of the g glycoprotein was reflected in an igg subclass immune response more like that to a ... | 1989 | 2715331 |
| characterization of prolonged apneic episodes associated with respiratory syncytial virus infection. | we used high-speed polygraphic recordings to document in detail the sequence of events during prolonged apneic spells in two infants with respiratory syncytial viral (rsv) infection. also, we elicited upper airway reflexes by stimulating the airway with saline during sleep. spontaneous prolonged apneic spells were recorded in both infants, and these consisted of two distinctly different apnea types. a number of similarities between apnea of prematurity, upper airway reflex apnea and the apneas i ... | 1989 | 2717245 |
| immunoglobulin-class-specific immune response to respiratory syncytial virus structural proteins in infants, children, and adults. | the protein specificities of igg, igm, and iga antibodies induced during respiratory syncytial virus (rsv) infection in 74 patients (4 weeks to 81 years of age) were investigated using the technique of immunoblotting. although the pattern of antibody reactivity varied among patients, most of the humoral immune response in all age groups was directed against the 48, 42, 35, and 27 k proteins. an infant's own antibody response was discernible in 55 of the 57 children below 1 year of age, despite t ... | 1989 | 2723614 |
| immune response to experimentally induced infection with respiratory syncytial virus: possible role in the development of pulmonary disease. | groups of mice were immunized intraperitoneally with hep-2 lysate or respiratory syncytial virus (rsv)-infected hep-2 cells with or without adjuvant(s). the animals were subsequently challenged intranasally (i.n.) with rsv and/or hep-2 lysate and studied for rsv shedding in the lung, antigen-specific antibody response and pulmonary histopathology. a significant decrease in virus shedding was detected in each of four groups of animals immunized with a virus preparation and in two of four groups t ... | 1989 | 2732693 |
| induction of in vitro differentiation of mouse embryonal carcinoma (f9) and erythroleukemia (mel) cells by herbimycin a, an inhibitor of protein phosphorylation. | herbimycin a is one of the benzenoid ansamycin antibiotics isolated from a culture of streptomyces species (omura, s., a. nakagawa, and n. sadakane. 1979. tetrahedron lett. 1979: 4323-4326). recent studies have shown that the antibiotic not only inhibits the phosphorylation of p60src in rous sarcoma virus- (rsv) infected cells, but also reverses the cellular phenotypes acquired by transfection with tyrosine kinase oncogenes (uehara, y., m. hori, t. takeuchi, and h. umezawa. 1985. jpn. j. cancer ... | 1989 | 2745553 |
| bronchial reactivity in infants in acute respiratory failure with viral bronchiolitis. | airway reactivity and the effects of bronchodilators in infants are controversial. we studied the response to bronchodilator treatment in 14 mechanically ventilated infants (mean age, 2.74 months; range, 0.6-5.9) in respiratory failure caused by respiratory syncytial virus (rsv)-associated bronchiolitis. sixteen infants without lung disease, undergoing elective surgery, provided normal values. maximum expiratory deflation flow-volume (dfv) curves were produced by manual inflation of the lungs wi ... | 1989 | 2748221 |
| [respiratory insufficiency in acute bronchiolitis in infancy]. | forty-one infants with acute viral bronchiolitis were hospitalized in our paediatric intensive care unit during the seven year period from 1980 to 1987. in 14 out of 27 evaluated patients, respiratory syncitial virus (rsv) was detected in the nasal secretions. twenty-three children required only supportive care and monitoring. eighteen infants had to be ventilated because of respiratory failure. the major indication for mechanical ventilation was an arterial or capillary pco2 of more than 64 mmh ... | 1989 | 2755928 |
| prevalence of respiratory syncytial virus subgroups over six consecutive outbreaks: 1981-1987. | indirect immunofluorescence with strain-specific monoclonal antibodies was used to determine the phenotype of respiratory syncytial virus (rsv) isolates obtained from infants hospitalized in greater boston over six successive outbreaks from 1981 to 1987. of 981 isolates, 591 (60%) were classified as subgroup a and 383 (39%) as subgroup b. the prevalence of subgroups varied both between and within yearly outbreaks. in 1983-84 and 1984-85, both subgroups circulated concurrently and in almost equal ... | 1989 | 2760479 |
| the frequency, distribution and effects of antibodies, to seven putative respiratory pathogens, on respiratory disease and weight gain in feedlot calves in ontario. | during 1983-85, 279 calves requiring treatment for bovine respiratory disease and 290 comparison (control) animals from 15 different groups of feedlot calves were bled on arrival and again at 28 days postarrival. their sera were then analyzed for antibodies to seven putative respiratory pathogens. on arrival, the prevalences of indirect agglutination titers to pasteurella haemolytica, p. haemolytica cytotoxin, mycoplasma bovis and m. dispar were greater than 50%, the prevalence of titers to bovi ... | 1989 | 2766158 |
| evidence that the fusion protein of respiratory syncytial virus exists as a dimer in its native form. brief report. | the quaternary structure of respiratory syncytial virus (rsv) fusion protein has been studied. crosslinking studies were done to stabilize the noncovalently associated proteins. these stable, heat-resistant, covalently linked complexes were analyzed by sodium dodecyl sulfate-polyacrylamide electrophoresis. in situ crosslinking studies demonstrated that the fusion protein of rsv exists as a dimer in its native form on the surface of infected cells. the purified protein was also found to be presen ... | 1989 | 2774976 |
| immunoglobulin a antibody response to respiratory syncytial virus structural proteins in colostrum and milk. | immunoglobulin a (iga) antibody response to respiratory syncytial virus (rsv) structural proteins in colostrum and milk was investigated by a radioimmunoprecipitation assay. by using [35s]methionine-labeled rsv-infected hep-2 cells and antiserum to human iga as the capture antibody, iga antibody responses to large glycoprotein, fusion protein, nucleoprotein, phosphoprotein, and matrix protein were demonstrated in colostrum and milk. the iga antibody response was mainly directed against fusion pr ... | 1989 | 2778061 |
| flow cytometric analysis of pulmonary lymphocytes from mice infected with respiratory syncytial virus. | balb/c mice were infected intra-nasally with respiratory syncytial virus (rsv) and cells recovered from the lungs by single or repeated bronchoalveolar lavage (bal). single bal gave enough cells (1 - 2 x 10(5) cells/mouse) for morphological study of giemsa stained cytospin preparations, and allowed the same mouse lungs to be assayed for virus titre or processed for histology. in normal or sham-infected mice the majority of recovered cells were macrophages, with less than 5% lymphocytes. followin ... | 1989 | 2784741 |
| interleukin-1 inhibitor production by human mononuclear leukocytes and leukocyte subpopulations exposed to respiratory syncytial virus: analysis and comparison with the response to influenza virus. | net interleukin-1 (il-1) inhibitor activity is induced by exposure of purified human monocytes-macrophages to respiratory syncytial virus (rsv). furthermore, il-1 inhibitor activity was produced by monocytes-macrophages exposed to rsv in the presence of lymphocytes, that is, by unseparated mononuclear leukocytes (mnl). purified rsv-exposed lymphocytes, as well as the lymphocytes exposed within mnl preparations, produced net il-1 inhibitor activity. in contrast, net il-1 activity was produced whe ... | 1989 | 2788203 |
| clinical characteristics of respiratory syncytial virus infections in healthy versus previously compromised host. | in an effort to delineate the clinical characteristics of respiratory syncytial virus (rsv) infection in the compromised host, we compared children with bronchopulmonary dysplasia (bpd), congenital heart disease (chd), premature birth, failure to thrive, and gastroesophageal reflux to previously healthy children. during a four-year period, 262 patients were admitted to the hospital with rsv infection diagnosed by a rapid rsv antigen detection test. children with bpd or chd had more hospital days ... | 1989 | 2797931 |
| duration of hospitalization in previously well infants with respiratory syncytial virus infection. | to describe the typical hospital course of infection in previously well infants hospitalized with respiratory syncytial virus (rsv) infection, we reviewed the charts of 196 patients with laboratory-proved respiratory syncytial virus infection in the 1987-1988 respiratory disease season. eighty-seven of the children had been previously well. their mean duration of hospitalization was 3.4 days. previously well infants younger than 6 weeks of age experienced significantly longer hospitalizations an ... | 1989 | 2797955 |
| effect of vaccination on cell populations in lung washes from calves after infection with respiratory syncytial virus. | the inflammatory response in the air-passages of the lungs of calves after intranasal inoculation with respiratory syncytial virus (rsv) was compared in rsv-vaccinated and control animals. total cells recovered from lung washings remained the same; however, the fold by eight days after infection and the type of cells changed from a predominance (85 per cent) of macrophages to equal proportions of macrophages and neutrophils (45 per cent) during the course of infection. the absolute numbers of ne ... | 1989 | 2799080 |
| hilar enlargement in respiratory syncytial virus pneumonia. | the clinical and radiographic features of ten children with hilar enlargement in association with proven respiratory syncytial virus (rsv) infection are described. hilar enlargement was seen in 10/35 children with rsv infection, and was invariably unilateral and right sided. it is recommended that rsv pneumonia be considered in children with unilateral hilar enlargement if tuberculosis has been excluded, and the onset of disease is rapid. | 1989 | 2806272 |
| antibody-mediated enhancement of respiratory syncytial virus infection in two monocyte/macrophage cell lines. | monoclonal antibodies (mabs) specific for two surface glycoproteins of respiratory syncytial virus (rsv) were found to enhance rsv infection in two macrophagelike cell lines (p388d1 and thp-1). mabs to an irrelevant antigen (pneumococcal polysaccharide) and to the nucleocapsid of rsv did not enhance infection. blocking either the fc segment of the monoclonal antibody of the fc receptor on the cells diminished the enhancement, suggesting that this phenomenon involves attachment of the monoclonal ... | 1989 | 2809253 |
| bacterial coinfection in children hospitalized with respiratory syncytial virus infections. | clinical and bacterial findings were prospectively studied in 90 children hospitalized because of middle or lower respiratory tract infection caused by respiratory syncytial virus (rsv) during a surveillance period of 12 months. the results were compared with those of rsv-negative children hospitalized with identical indications during the 3 peak months of the rsv epidemic (n = 91) or for the 3 months after the outbreak (n = 99). a high frequency of pneumonia and acute otitis media were found in ... | 1989 | 2812913 |
| respiratory syncytial virus infections in children admitted to the intensive care unit. | during the winter of 1986-1987, 64 children with respiratory syncytial virus (rsv) infection were admitted to our hospital. the diagnosis was made by direct immunofluorescent antibody technique. twenty-three children (36%) needed intensive care treatment. nearly 11 (52%) had a preexisting disease state, identified as a risk factor i.e., prematurity (n = 8), bronchopulmonary dysplasia (n = 2), congenital heart disease (n = 1). twelve patients (50%) were intubated and ventilated. conditions for in ... | 1989 | 2812976 |
| a mutation in v-src that removes a single conserved residue in the sh-2 domain of pp60v-src restricts transformation in a host-dependent manner. | the v-src oncogene of rous sarcoma virus (rsv) is able to transform both avian and mammalian cells, but the mutant allele v-src-l displays a host range dependence for transformation, transforming chicken but not rat cells with wild-type efficiency. this host range restriction can be detected by measuring growth in low serum, saturation density, and anchorage independent growth. in addition, rat cells expressing v-src-l do not form tumors in syngeneic rats or nude mice, but rsv carrying the mutan ... | 1989 | 2462061 |
| isolation of a catalytically competent phosphorylated tyrosine kinase from rous sarcoma virus-induced rat tumor by immunoadsorption to and hapten elution from phosphotyrosine binding antibodies. | a procedure has been developed for the isolation of a catalytically competent phosphorylated tyrosine kinase (rsv y-kinase) from avian sarcoma virus-induced rat tumors. the procedure involves reaction of partially purified rsv y-kinase with atp to effect tyrosyl phosphorylation of catalytically competent rsv y-kinase. tyrosyl phosphorylated rsv y-kinase was isolated from the heterogeneous reaction mixture by immunoadsorption on immobilized phosphotyrosyl binding antibodies and elution with the h ... | 1990 | 1690027 |
| phosphorylation of connexin43 gap junction protein in uninfected and rous sarcoma virus-transformed mammalian fibroblasts. | gap junctions are membrane channels that permit the interchange of ions and other low-molecular-weight molecules between adjacent cells. rous sarcoma virus (rsv)-induced transformation is marked by an early and profound disruption of gap-junctional communication, suggesting that these membrane structures may serve as sites of pp60v-src action. we have begun an investigation of this possibility by identifying and characterizing putative proteins involved in junctional communication in fibroblasts ... | 1990 | 1690850 |
| demonstration of respiratory syncytial virus in an autopsy series. | respiratory syncytial virus (rsv) antigen was demonstrated in formalin-fixed, paraffin-embedded autopsy tissue using an immunoperoxidase technique. eighteen autopsy cases were selected on the basis of one of the following criteria: a positive culture for rsv, antemortem or postmortem; positive elisa test for rsv, antemortem or postmortem; or postmortem histology suggestive of paramyxovirus infection. controls included three cases from which parainfluenza or influenza virus had been cultured and ... | 1990 | 1695371 |
| [recombinant rna-dependent dna-polymerase from rous sarcoma virus. isolation and properties]. | preparations of recombinant reverse transcriptase rsv were isolated from e. coli hb101/pmf14 cell cultures. the enzyme purified to homogeneity was shown to be made up of two subunits with molecular masses of 97 +/- 4 and 61 +/- 3 kda. a comparison of enzymatic properties of recombinant transcriptase to those of the enzyme isolated from the rsv (rauss sarcoma) virus demonstrated that in the preparations under study the recombinant reverse transcriptase exists in a subunit form, alpha beta, and ma ... | 1990 | 1696135 |
| purification and properties of extracellular matrix-degrading metallo-proteinase overproduced by rous sarcoma virus-transformed rat liver cell line, and its identification as transin. | rous sarcoma virus-transformed rat liver cell line rsv-brl secreted a neutral proteinase in a latent precursor form with a molecular weight (mr) of 57,000 (57k) as a major secreted protein. this enzyme was a calcium-dependent metallo-proteinase. the proenzyme was purified from the serum-free conditioned medium of the transformed cells by affinity chromatographies on a zinc chelate sepharose column and a reactive red agarose column. when activated by treatment with trypsin or p-aminophenylmercuri ... | 1990 | 1963430 |
| viruses associated with acute lower respiratory tract infections in children from the eastern highlands of papua new guinea (1983-1985). | this study, conducted at goroka hospital from january 1983 to june 1985, examined the viruses identified in nasopharyngeal aspirates (npa) and urines collected from 716 hospitalised children with moderate or severe pneumonia, in npa from 170 children with mild pneumonia treated as outpatients and in npa from a control group of 428 children attending the outpatient department of goroka hospital suffering from minor ailments other than upper or lower respiratory tract infections. one or more virus ... | 1990 | 1963705 |
| evaluation of the toxicity and antiviral activity of carbocyclic 3-deazaadenosine against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. | the toxicity and antiviral efficacy of carbocyclic 3-deazaadenosine (cc3ado) against respiratory syncytial (rsv) and parainfluenza type 3 (piv3) virus infections were tested in tissue culture and in cotton rats. the mean median efficacious dose (ed50) of cc3ado in hep2 cells against rsv and piv3 was 9 and 14 micrograms/ml, respectively. these values were 85- and 55-fold less than the median inhibitory (toxic) dose (id50) of cc3ado in this cell line (750 micrograms/ml), and similar to values obta ... | 1990 | 1965109 |
| toxicity and antiviral activity of ly253963 against respiratory syncytial and parainfluenza type 3 viruses in tissue culture and in cotton rats. | ly253963, the sodium salt of 1,3,4-thiadiazol-2-ylcyanamide, was evaluated in tissue culture and in cotton rats for toxicity and antiviral efficacy against respiratory syncytial (rsv) and parainfluenza type 3 (piv3) viruses. the selective index (ratio of the median toxic dose: median efficacious dose) of ly253963 in hep2 tissue culture cells was greater than 100 against both rsv and piv3. when given intraperitoneally to cotton rats, the minimum protective dose of ly253963 against both of these v ... | 1990 | 1965110 |
| transmembrane interactions at cell adhesion and invasion sites. | chicken embryonic fibroblasts transformed by rous sarcoma virus (rsv-cef) invade into a film of the extracellular matrix (ecm) by extending membrane protrusions, termed the invadopodia. the invadopodia share similar cytoskeletal components and membrane receptors for ecm components as adhesion sites. however, the organization of these transmembrane components at invadopodia and adhesion sites differs. in addition, degradation of the ecm occurs at sites of the invadopodia, but not at focal adhesio ... | 1990 | 1965957 |
| analysis of b complex polymorphism in rous sarcoma progressor and regressor chickens with b-g, b-f, and b-l beta probes. | molecular polymorphism of the b complex was studied in serologically defined b19 haplotypes by use of class i, class ii, and class iv probes in southern blot experiments in chickens. all chickens studied shared identical class iv restriction patterns. in contrast, class i and class ii probes revealed six and five subtypes of b19 haplotype, respectively. these subtypes may be resolved in three homozygous genotypes and their corresponding heterozygous combinations. previous genetic selection allow ... | 1990 | 1980812 |
| [ultrastructural changes in middle ear and cochlea caused by respiratory syncytial virus in guinea pigs]. | respiratory syncytial virus (rsv) was inoculated into the middle ear of guinea pigs. preyer's reflex began to decrease since 21st day. the positive rate of rsv culture from middle ear mucosa within the first week was 50%. the titer of igg antibody against rsv in sera rose gradually. electron microscopy showed enlarged rough endoplasmic reticulum, swelling mitochondria and pyknosis of nuclei in the epithelial and mononuclear cells of middle ear. the surface of corti's organ was covered with exuda ... | 1990 | 2076325 |
| primary pulmonary murine cytotoxic t lymphocyte specificity in respiratory syncytial virus pneumonia. | effector cells capable of lysing respiratory syncytial virus (rsv)-infected cells were isolated from the lungs of intranasally infected mice. an examination of their specificity showed that cytolysis was major histocompatibility complex restricted. using recombinant vaccinia viruses containing cloned rsv genes to infect target cells, balb/c (h-2d) pulmonary effector cells were shown to recognize the fusion protein (f) and to a lesser extent the nucleoprotein (n). cells specific for the major gly ... | 1990 | 2077342 |
| [seroepidemiologic studies of the distribution of respiratory syncytial virus in wild ruminants]. | sera from wild ruminants (302 roe, 39 red deer, 17 fallow deer, 1 mouflon) were tested for antibodies against respiratory syncytial virus (rsv). 22 sera (6.1%) were positive: 20 roe, 1 red deer, 1 mouflon. the findings are discussed from the epizootiological point of view. | 1990 | 2078187 |
| [the role of respiratory tract viruses in the etiology of obstructive bronchitis in infants]. | out of 524 children with acute respiratory infections in 141 obstructive bronchitis was diagnosed (ozo). seventy cases could be linked to viral infection. viral infections tested (influenza virus a, b, parainfluenza typ 1-3, rsv, adenoviruses) were more frequently associated with ozo than other acute respiratory infections of unknown etiology. majority infections induced by influenza virus a and parainfluenza virus typ 2 were accompanied by ozo symptoms. of the highest risk of acquiring ozo desp ... | 1990 | 2084449 |
| comparison of lung histopathology and bronchoalveolar lavage cytology in mice and cotton rats infected with respiratory syncytial virus. | lung histology as well as cell number and size distribution in bronchoalveolar lavage (bal) were compared in balb/c mice and in cotton rats, of various immune status regarding the respiratory syncytial virus (rsv), when subjected to challenge with rsv. in mock-immunized animals, rsv infection typically caused microscopic inflammatory lesions of the lungs and the presence of inflammatory cells in the bal. immunization with a formalin inactivated vaccine prior to challenge increased the severity o ... | 1990 | 2087875 |
| [evaluation of methods for the detection of syncytial respiratory virus in nasopharyngeal secretions]. | the screening for respiratory syncytial virus (rsv) in nasopharyngeal secretions with enzyme immunoassay (elisa) and indirect immunofluorescence (iif) has been evaluated in infants and young children with acute respiratory infection. both methods were compared with viral isolation in hep-2 cells and the investigation of fluorescent foci in cell cultures inoculated by centrifugation. 226 samples were evaluated by iff, 182 of which were also evaluated by elisa while 158 were inoculated into cell c ... | 1990 | 2098144 |
| [pneumonia in adults caused by the respiratory syncytial virus]. | respiratory syncytial virus (rsv) usually affects children but sometimes affects a certain group of adults. a case of an 81 year old female with idiopathic thrombocytopenic purpura who had pneumonia caused by rsv is presented. the literature is reviewed as well as the most frequent clinical manifestations, diagnosis methods and treatment. | 1990 | 2103289 |
| targeted immunoglobulin therapy for the prevention of neonatal infections. | premature infants demonstrate hypoglobulinemia and are at increased risk for serious infections. although a cause-and-effect relation between low serum igg levels and neonatal infections has not been established, prophylaxis of such severe infections may be possible by replacement of antibody with intravenous immunoglobulin (ivig). for success, ivig must provide specific antibodies to neonatal pathogens and reach therapeutic serum igg target levels. pilot investigations have demonstrated that iv ... | 1990 | 2114035 |
| acute and long-term effects of viral bronchiolitis in infancy. | about 1% of infants are admitted to hospital with acute bronchiolitis; 85% of cases are caused by infection with respiratory syncytial virus (rsv). the pathophysiological changes during the acute illness are inflammatory obstruction in the small airways with submucosal cellular infiltration, epithelial necrosis and mucous plugging; frc increases and dynamic compliance falls. failure to respond to bronchodilator drugs suggests that muscle spasm contributes relatively little to the airway narrowin ... | 1990 | 2117143 |
| economic and long-term benefits of ribavirin therapy on respiratory syncytial virus infection. | ribavirin is a broad-spectrum antiviral agent. administered as an aerosol, it has been shown to be clinically effective in improving the signs and symptoms of viral bronchiolitis in infancy, particularly cases due to respiratory syncytial virus (rsv). this paper reviews the evidence for economic and/or long-term clinical benefits from using ribavirin in the acute illness. there are data to suggest that use of ribavirin may lead to a reduction in therapeutic interventions and duration of hospital ... | 1990 | 2117144 |
| ig/ebp-1: a ubiquitously expressed immunoglobulin enhancer binding protein that is similar to c/ebp and heterodimerizes with c/ebp. | we report the isolation and characterization of cdna clones that encode a protein with the same dna binding specificity as the immunoglobulin heavy chain enhancer binding protein e (muebp-e). we call the gene encoding this protein ig/ebp-1. a fusion protein encoded by the cdna binds specifically to muebp-e-binding sites (e sites) in both the igh enhancer and the vh1 promoter. sequence analysis reveals that ig/ebp-1 is a member of the "basic-zipper" family of dna-binding proteins that are charact ... | 1990 | 2121606 |
| overexpression of avian or mouse c-jun in primary chick embryo fibroblasts confers a partially transformed phenotype. | the coding sequences of avian (quail) or murine c-jun proto-oncogenes were introduced into a non-defective retroviral vector derived from rous sarcoma virus (rsv) in which c-jun replaces v-src. primary avian fibroblasts chronically infected with either one of these viruses exhibit some phenotypic traits characteristic of rsv-transformed cells, including sustained growth in low serum medium and ability to develop colonies from single cells in agar, even though they are still of normal morphology ... | 1990 | 2123532 |
| a study of nonbacterial agents of acute lower respiratory tract infection in thai children. | from january 1986 to december 1987, 596 children less than 5 years of age with lower respiratory tract infection (lri)--manifested as laryngitis, croup, bronchitis, bronchiolitis, and pneumonia--were studied for evidence of infection with respiratory tract viruses mycoplasma pneumoniae, and chlamydia trachomatis. of the 596 children in the study, 315 were ambulatory and 281 were hospitalized. virologic studies included isolation and rapid diagnosis of virus from specimens of nasopharyngeal aspir ... | 1990 | 2125359 |
| respiratory syncytial virus modulation of adult and neonatal lymphocyte mitogenic responses and the role of interferon-gamma. | infection of human adult and neonatal mononuclear leukocytes (mnls) with respiratory syncytial virus (rsv) caused significant inhibition of lymphoproliferation in response to various mitogens (heat-killed staphylococcus aureus, phytohaemagglutinin, concanavalin-a and pokeweed mitogen). antibody to human interferon-gamma enhanced the rsv-induced suppression of lymphoproliferation in adult, but not neonatal mnls. these results are consistent with the hypothesis that rsv can suppress the developmen ... | 1990 | 2129048 |
| lymphocyte subpopulations in peripheral blood of lambs experimentally infected with bovine respiratory syncytial virus. | the lymphocyte subpopulations of peripheral blood of normal lambs and lambs experimentally infected with bovine respiratory syncytial virus (rsv) were analysed by flow cytometry, using a panel of monoclonal antibodies against specific lymphocyte epitopes. experimental infection with bovine rsv was characterized by a significant rise in sbu-t8+ (cd8+ or cytotoxic) t cells and a significant reduction in sbu-t4+ (cd4+ or helper) t cells and b (lca p220+) lymphocytes (p less than 0.05). the helper/s ... | 1990 | 2140217 |
| coding strategy of rice stripe virus: major nonstructural protein is encoded in viral rna segment 4 and coat protein in rna complementary to segment 3. | the two major proteins found in plants infected with rice stripe virus (rsv), coat protein and a major nonstructural protein (major ns), were purified and their partial amino acid sequences were determined. oligonucleotides were synthesized according to the amino acid sequence information, and used as probes for northern blot analyses of four single-stranded rna species (segments 1-4) and four double-stranded rna species of rsv. the results indicated that the coding strategy of rsv was similar t ... | 1990 | 2141205 |
| protection from herpes simplex virus type 1 lethal and latent infections by secreted recombinant glycoprotein b constitutively expressed in human cells with a bk virus episomal vector. | the herpes simplex virus type 1 (hsv-1) glycoprotein b (gb-1) gene, deleted of 639 nucleotides that encode the transmembrane anchor sequence and reconstructed with the extramembrane and intracytoplasmic domains, was cloned under control of the rous sarcoma virus long terminal repeat in the episomal replicating vector prp-rsv, which contains the origin of replication and early region of the human papovavirus bk as well as a cdna for a mutant mouse dihydrofolate reductase that is resistant to meth ... | 1990 | 2152829 |
| identification of a third protein factor which binds to the rous sarcoma virus ltr enhancer: possible homology with the serum response factor. | we have identified a new protein factor (efiii) in nuclear extracts of quail fibroblasts and chick embryos which binds specifically in vitro to a 26-bp region of the rous sarcoma virus (rsv) long terminal repeat (ltr) enhancer. the efiii binding site in the rsv ltr exhibits a strong sequence homology to the serum response element (sre). the sre is a 22-bp cis-acting dna sequence element, first identified upstream of the human c-fos gene, which can confer serum inducibility to heterologous promot ... | 1990 | 2152992 |
| plasminogen activator gene expression is induced by the src oncogene product and tumor promoters. | secretion of urokinase-type plasminogen activator (upa) by chicken embryo fibroblasts (cef) is increased approximately 50-fold following transformation by rous sarcoma virus (rsv). using a cloned and fully sequenced chicken upa cdna probe, we have established that this increase in plasminogen activator production can be largely accounted for by an increase in cellular upa mrna. cef contained on average less than 1 molecule of upa mrna/cell, whereas rsv-cef contained 25-60 molecules/cell. the inc ... | 1990 | 2153128 |
| localization of 9e3/cef-4 in avian tissues: expression is absent in rous sarcoma virus-induced tumors but is stimulated by injury. | the avian gene 9e3/cef-4, a member of the superfamily of genes that includes kc and gro, is expressed abundantly in exponentially growing cultures of chick embryo fibroblasts (cefs) and at high levels in cefs transformed with rous sarcoma virus (rsv). the product of this gene is a secreted protein that has homologies and structural similarities to inflammatory mediators. the function of 9e3 is obscure and its expression in vivo has not yet been investigated. we studied by in situ hybridization a ... | 1990 | 2155240 |
| a study of the dimer formation of rous sarcoma virus rna and of its effect on viral protein synthesis in vitro. | the genetic material of all retroviruses examined so far is an rna dimer where two identical rna subunits are joined at their 5' ends by a structure named dimer linkage structure (dls). since the precise location and structure of the dls as well as the mechanism and role(s) of rna dimerization remain unclear, we analysed the dimerization process of rous sarcoma virus (rsv) rna. for this purpose we set up an in vitro model for rsv rna dimerization. using this model rsv rna was shown to form dimer ... | 1990 | 2155394 |
| glycyl-l-glutamine stimulates the accumulation of a12 acetylcholinesterase but not of nicotinic acetylcholine receptors in quail embryonic myotubes by a cyclic amp-independent mechanism. | myotubes prepared from the japanese quail embryo at 9 days gestation were cultivated in the presence of glycyl-l-glutamine (gly-gln, beta-endorphin c-terminal dipeptide) or glycyl-glutamic acid (gly-glu), and changes in the activity of acetylcholinesterase (ache) molecular forms and binding of 125i-alpha-bungarotoxin (alpha bgt) to cell surface nicotinic acetylcholine receptors were measured. the a12 oligomer was the major form of ache in the cultures. the activity of all molecular forms of the ... | 1990 | 2156012 |
| surveillance of viral respiratory tract infections over a one year period in mainly hospitalized austrian infants and children by a rapid enzyme-linked immunosorbent assay diagnosis. | in order to obtain more information on viral respiratory tract infections in austrian infants and children, nasopharyngeal secretions from 1432 infants and children, collected from october 1984 to october 1985, were screened for the presence of respiratory syncytial virus (rsv), adenoviruses, parainfluenza virus type 1, 2, and 3, and influenza viruses type a and b, by enzyme-linked immunosorbent assay (elisa). the results obtained were analyzed with respect to incidence, seasonal distribution an ... | 1990 | 2156381 |
| evaluation of homology modeling of hiv protease. | the model of human immunodeficiency virus (hiv-1) protease which was based on the crystal structure of rous sarcoma virus (rsv) protease has been compared to the recently determined crystal structure of chemically synthesized hiv-1 protease. the overall difference between the model and crystal structure was 1.4 a root mean square (rms) deviation for 86 superimposed c alpha atoms. the position of the flexible flap differs in the model and six residues at the amino terminus were incorrectly placed ... | 1990 | 2158092 |
| serum and v-src increase the level of a ccaat-binding factor required for transcription from a retroviral long terminal repeat. | transcription from the long terminal repeat (ltr) of rous sarcoma virus (rsv) in rat 3y1 fibroblasts was dependent on the presence of serum. within 1 hr after addition of serum to a serum-deprived culture, there was a fivefold increase in the level of transcripts initiated at the ltr. this stimulation did not require synthesis of new proteins. the induction of transcription by serum was mostly dependent on two ccaat boxes in the ltr. within 1 hr after addition of serum, there was also an increas ... | 1990 | 2159932 |
| localization by mutational analysis of transcription factor binding sequences in the u3 region of rous sarcoma virus ltr. | the transcription factor binding sequences in the u3 region of rous sarcoma virus ltr have been determined by gel retardation assays using mutant synthetic oligonucleotides. the results indicate that the factor, e2bp, specifically binds to sequences tgcaatac and tgcaacat, which are localized between nucleotides -222 to -215 and -203 to -196, respectively. this factor is present at elevated levels in avian qt6 cells compared to mouse 3t3 and rat 2 tk- cells. e2bp binds to a sequence that is simil ... | 1990 | 2161147 |
| distinct and different effects of the oncogenes v-myc and v-src on avian sympathetic neurons: retroviral transfer of v-myc stimulates neuronal proliferation whereas v-src transfer enhances neuronal differentiation. | immature avian sympathetic neurons are able to proliferate in culture for a limited number of divisions albeit expressing several neuron-specific properties. the effect of avian retroviral transfer of oncogenes on proliferation and differentiation of sympathetic neurons was investigated. primary cultures of 6-d-old quail sympathetic ganglia, consisting of 90% neuronal cells, were infected by myelocytomatosis virus (mc29), which contains the oncogene v-myc, and by the v-src-containing rous sarcom ... | 1990 | 2161856 |
| transcriptional activity of the rous sarcoma virus long terminal repeat correlates with binding of a factor to an upstream ccaat box in vitro. | the avian nuclear protein, enhancer factor 1 (ef1), binds specifically to the long terminal repeat (ltr) of rous sarcoma virus (rsv) in a region that has been implicated in enhancer/promoter function. we have characterized the in vitro binding properties of this factor from chick embryo nuclear extracts by methylation interference/protection foot-printing of the wild-type ltr and also by gel electrophoretic mobility shift assays performed on a series of ltr mutants. we find that the inverted cca ... | 1990 | 2162108 |
| [molecular basis of retrovirus adaptation: nucleotide sequence of rous sarcoma virus adapted to duck cells]. | the host range of retroviruses is rather complex and specific. it is controlled by the products of viral structural genes that interact with the determinants both on the surface and within the cell. the possibility to infect and transform duck embryo fibroblasts is shown for the prague strain of chicken rous sarcoma virus (subgroup c), though virus production in these cells is restricted. however, after the 6th passage the "adapted" virus gave the titre practically the same as it was for chicken ... | 1990 | 2162309 |
| comparison of the crystal structures and intersubunit interactions of human immunodeficiency and rous sarcoma virus proteases. | the crystal structures of the proteases (prs) encoded by the rous sarcoma virus (rsv) and the human immunodeficiency virus (hiv) have been compared. the crystallographic monomer of hiv pr superimposes on the two crystallographically independent subunits of the rsv pr dimer with root mean square deviations of 1.45 and 1.55 a for 86 and 88 common c alpha atoms, respectively. there is a conserved structural core consisting of seven beta-strands forming two perpendicular layers, a helix, and the ami ... | 1990 | 2162350 |