Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| synthesis of some 5'-amino-2',5'-dideoxy-5-iodouridine derivatives and their antiviral properties against herpes simplex virus. | in an attempt to improve the antiviral efficacy of 5'-amino-2',5'-dideoxy-5-iodouridine (aidu) the n-acetyl and n,3'-o-diacetyl derivatives were prepared. n-acetylation of aidu increased its ability to inhibit the phosphorylation of thymidine by the deoxypyrimidine kinase of herpes simplex virus type 1 (hsv1) while diacetylation had the converse effect. the affinity of the corresponding compounds containing uracil or thymine for virus deoxypyrimidine kinase was also determined. a range of n-acyl ... | 1982 | 6299187 |
| cell-mediated immunity in mice with primary, secondary and latent herpes simplex virus infection. | cell mediated immunity was studied by a cytopathic effect inhibition assay in mice infected in the ear with herpes simplex virus type 1 (hsv 1). activity appeared rapidly, reaching a high level 6 days after primary infection. it had fallen 10 days after infection and was undetectable during latency, 3-5 weeks after infection. the activity reappeared even more rapidly and strongly after reinoculation with the virus, but stimuli designed to induce recurrent disease did not induce clinical disease ... | 1982 | 6299234 |
| effect of acyclovir and presence of cellular and viral thymidine kinase activity in herpes simplex virus infected cells. brief report. | herpes simplex type 1 (hsv-1) infected human lung fibroblasts show a higher viral thymidine kinase activity than infected african green monkey kidney cells. this may partly account for the higher antiviral activity of acyclovir in the former cells. | 1982 | 6299236 |
| deoxythymidine kinases in varicella-zoster virus infected and biochemically transformed cells. | deoxythymidine kinase (tk) activity induced in varicella-zoster virus (vzv)-infected human embryonic fibroblast (hef) cells was immunologically distinguishable from that in non infected hef cells and also from that in herpes simplex virus type 1 (hsv-1) infected hef cells. the tks in vzv-biochemically transformed cells were immunologically the same as that induced in vzv-infected human cells and immunologically different from that in ltk- cells or in hsv-biochemically transformed cells. one peak ... | 1982 | 6299267 |
| comparison of restriction endonuclease profiles of dna from local herpes simplex virus type 2 (hsv-2) strains. | comparisons of restriction endonuclease profiles of dna isolated from nine genital hsv-2 strains isolated from 1973 to 1981 were made using six restriction endonucleases (bgl ii, hind iii, xba 1, eco ri, bam hi and hpa i). the only variation detected in the dna profiles was found with eco ri and bam hi, and in each case all the isolates could be classified into two groups. the difference between the two eco ri groups could be accounted for by the absence of an eco ri cleavage site from the hsv d ... | 1982 | 6293436 |
| modifications of the nuclear envelope of bhk cells after infection with herpes simplex virus type 1. | numerous discrete lesions, which we have termed blebs, appeared in the nucleus of bhk cells 10 to 15 h after infection with herpes simplex virus type 1 (hsv-1). they were formed in the inner portion of the nuclear envelope by the apposition of two thickened lamellae overlying a vacuole. as demonstrated by electron microscopic studies, blebs were regular and associated with the peripheral lamina in the nucleus, averaging 3.5 blebs per micron2. they appeared to be associated with an enrichment of ... | 1982 | 6294235 |
| identification and nucleotide sequences of two similar tandem direct repeats in epstein-barr virus dna. | epstein-barr virus dna is known to have partially homologous segments, designated dl and dr, near the left and right ends of the long unique region (raab-traub et al., cell 22:257-267, 1980). dl and dr are each partially composed of tandem direct repeat sequences. dl contains 11 to 14 repeats of a 124-base-pair sequence designated ir2. dr contains approximately 30 direct repeats of a 103-base-pair sequence designated ir4. the dl and dr sequences have colinear partial homology for approximately 2 ... | 1982 | 6294332 |
| transfer of human globin genes to erythroleukemic mouse cells. | thymidine kinase negative (tk-) friend cells were transformed with recombinant molecules carrying human globin genes and the thymidine kinase gene of herpes simplex virus type 1 dna. transformation frequencies of 1 transformant/microgram donor dna/1 x 10(6) cells were obtained by standard procedures and this was increased 20- to 30-fold by treating recipient cells with dimethyl sulfoxide or glycerol. transformed cell lines expressed thymidine kinase activity of viral origin as determined by its ... | 1982 | 6325149 |
| the immediate-early mrna that encodes the regulatory polypeptide vmw 175 of herpes simplex virus type 1 is unspliced. | the structure and precise map location of the 4.1-kb herpes simplex virus type 1 (hsv-1) immediate-early mrna (ie mrna-3) that encodes a regulatory polypeptide, with an approximate mol. wt. of 175 000, has been determined. nuclease s1 digestion procedures using labelled virus dna probes have shown that ie mrna-3 is unspliced and maps entirely within the trs/irs genome regions. dna sequences at the 5' and 3' ends of ie mrna-3 have been examined and regulatory signals involved in initiation of tra ... | 1982 | 6327260 |
| localization of an origin of dna replication within the trs/irs repeated region of the herpes simplex virus type 1 genome. | an assay has been developed and used to locate an origin of dna replication on the herpes simplex virus type 1 (hsv-1) genome. baby hamster kidney cells were transfected with circular plasmid molecules containing cloned copies of hsv-1 dna fragments, and helper functions were provided by superinfection with wild-type hsv-1. the presence of an hsv-1 origin of replication within a plasmid enabled amplification of the vector dna sequences, which was detected by the incorporation of [32p]orthophosph ... | 1982 | 6329712 |
| differential effects of e-5-(2-bromovinyl)-2'-deoxyuridine on infections with herpes simplex virus type 1 and type 2 in hairless mice. | 1982 | 7054316 | |
| in vitro metabolism of 1-beta-d-arabinofuranosylcytosine and 1-beta-2'-fluoroarabino-5-iodocytosine in normal and herpes simplex type 1 virus-infected cells. | phosphorylation of 1-beta-d-2'-f-arabino-5-iodocytosine (fiac), a newly synthesized pyrimidine nucleoside with potent antiherpesvirus activity, was compared with that of its parent compound, 1-beta-d-arabinofuranosylcytosine (ara-c). while ara-c was phosphorylated extensively by homogenates of normal, rapidly proliferating mouse tissues, fiac was a poor substrate for the nucleoside kinase occurring in such normal tissues. with cell homogenates of noninfected vero cells, thymidine (tdr) was phosp ... | 1982 | 7082345 |
| carbocyclic analogue of 3-deazaadenosine: a novel antiviral agent using s-adenosylhomocysteine hydrolase as a pharmacological target. | the carbocyclic analogue of 3-deazaadenosine (3-deaza-c-ado) has been synthesized and found to have antiviral activity in cell culture against herpes simplex virus type 1, vaccinia virus, and hl-23 c-type virus. it is relatively noncytotoxic at effective antiviral concentrations and is not subject to deamination or phosphorylation. it acts as a competitive inhibitor of s-adenosyl-l-homocysteine hydrolase, is at best a poor substrate, and does not inactivate the enzyme significantly. 3-deaza-c-ad ... | 1982 | 7097716 |
| optimal treatment of herpes simplex virus encephalitis in mice with oral acyclovir. | the effect of oral or intraperitoneal administration of acyclovir was evaluated in four experimental models of herpes simplex virus (hsv) encephalitis in mice. mice were inoculated with hsv-1 or hsv-2 intracerebrally or with hsv-2 intranasally, intraperitoneally, or intravaginally. with all four routes of inoculation, oral acyclovir therapy significantly reduced mortality when started as late as 72 to 96 hours after viral challenge. intraperitoneal acyclovir was not effective in protecting mice ... | 1982 | 7102699 |
| purification and partial characterization of another form of the antiviral protein from the seeds of phytolacca americana l. (pokeweed). | 1. the pokeweed antiviral protein, previously identified in two forms (pap and pap ii) in the leaves of phytolacca americana (pokeweed) [obrig. irvin & hardesty (1973) arch. biochem. biophys. 155, 278-289; irvin, kelly & robertus (1980) arch. biochem. biophys. 200, 418-425] is a protein that prevents replication of several viruses and inactivates ribosomes, thus inhibiting protein synthesis. 2. pap is present in several forms in the seeds of pokeweed. one of them, which we propose to call 'pokew ... | 1982 | 7103950 |
| an adoptive cell transfer system for the evaluation of immunity to herpes simplex virus in mice. | lymphocytes adoptively transferred from syngeneic immune donors protect mice against challenge with herpes simplex virus type 1. normal mice require transfer 3 x 10(7) spleen cells for protection. sublethal irradiation (450 rad) decreases the number required five-fold. lymphocytes from non-immune donors do not protect, and hyperimmunization does not enhance the protection efficiency of donors. the viral ld50 varies through a range 10(5)-fold during the period of recovery from this amount of radi ... | 1982 | 7105488 |
| quantitative autoradiographic mapping of herpes simplex virus encephalitis with a radiolabeled antiviral drug. | 2'-fluoro-5-methyl-l-beta-d-arabinosyluracil (fmau) labeled with carbon-14 was used to image herpes simplex virus type 1-infected regions of rat brain by quantitative autoradiography. fmau is a potent antiviral pyrimidine nucleoside which is selectively phosphorylated by virus-coded thymidine kinase. when the labeled fmau was administered 6 hours before the rats were killed, the selective uptake and concentration of the drug and its metabolites by infected cells (defined by immunoperoxidase stai ... | 1982 | 7112121 |
| the development of retinitis in mice with nonfatal herpes simplex encephalitis. | a mutant strain of herpes simplex virus, type 1 (hsv1), selected for high resistance to acyclovir (acv) was inoculated intracerebrally into mice. the mice survived with no obvious neurological signs but developed cataracts within 4-8 weeks of inoculation. histological examination revealed only a mild encephalitis, but around 7 days after injection a florid, necrotizing, viral retinitis developed. there was almost simultaneous involvement of both eyes. inflammatory cell infiltration and early mye ... | 1982 | 7133314 |
| treatment of experimental herpes simplex virus encephalitis with (e)-5-(2-bromovinyl)-2'-deoxyuridine in mice. | (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) was examined for its ability to increase the survival rate of mice infected intracerebrally with herpes simplex virus type 1 (strain kos). bvdu was administered orally (through the drinking water), intraperitoneally, or subcutaneously at doses ranging from 40 to 400 mg/kg per day, starting 0, 2, 4, or 6 days postinfection. regardless of the route of administration, bvdu effected a significant reduction in the mortality rate of mice infected with herpes ... | 1982 | 7137983 |
| activity of 2-(3,4-dichlorophenoxy)-5-nitrobenzonitrile (mdl-860) against picornaviruses in vitro. | the newly synthesized compound 2-(3,4-dichlorophenoxy)-5-nitrobenzonitrile (mdl-860) has been found to inhibit picornavirus replication. in multiple growth cycle experiments, 1 microgram of mdl-860 per ml caused a reduction in virus yield of at least 1.0 log10 50% tissue culture infectious doses per 0.2 ml for 8 of 10 enteroviruses and 72 of 90 rhinovirus serotypes. this antiviral activity was dependent on both compound concentration and virus inoculum size. at concentrations that had no toxic e ... | 1982 | 7181475 |
| chemotherapeutic efficacy of e-5-(2-bromovinyl)-2'deoxyuridine for orofacial infection with herpes simplex virus type 1 in mice. | systemic or topical treatment with e-5-(2-bromovinyl)2'-deoxyuridine (bvdu) showed significant efficacy against orofacial infection with herpes simplex virus type 1 (hsv-1) in hairless mice. the chemotherapeutic response to bvdu was dose-dependent and clearly evident even when the treatment was initiated during the clinical manifestation of hsv-1 infection at 72 hr after inoculation. early initiation of therapy with bvdu at 3 or 24 hr after inoculation significantly prevented the establishment o ... | 1982 | 6979592 |
| intracameral inoculation of herpes simplex virus type i induces anterior chamber associated immune deviation. | herpes simplex virus type 1 (hsv-1) inoculated intracamerally (ic) into the anterior chamber of balb/c eyes induces anterior chamber associated immune deviation (acaid) in which t cell-mediated delayed type hypersensitivity (dth) responses to hsv-1 are impaired while b cell-mediated anti-hsv neutralizing antibody responses are intact or enhanced. | 1982 | 6985281 |
| [short-term therapeutic effect of an inactivated herpes simplex virus type 1 vaccine on herpes simplex keratitis]. | 1983 | 6327206 | |
| clinical and experimental study of the immune complex (herpes simplex virus type 1-igm) in herpes encephalitis brain. | 1983 | 6326643 | |
| novel use of synthetic oligonucleotide insertion mutants for the study of homologous recombination in mammalian cells. | thymidine kinase-deficient mouse l cells have been transformed with plasmid dnas carrying 8-base-pair xho i linker insertion mutations in the coding region of the herpes simplex virus type 1 thymidine kinase gene. when the mutant plasmids are introduced individually into ltk- cells, transformation efficiencies are greatly reduced relative to the wild type. however, when two mutant plasmids are cotransferred into the same ltk- recipients, significantly higher frequencies of transformation are obs ... | 1983 | 6576360 |
| evidence for intrachromosomal gene conversion in cultured mouse cells. | in this report we present an experimental scheme that facilitates the study of homologous recombination between closely linked genes in cultured mammalian cells. two different xho i linker insertion mutants of the herpes simplex virus type 1 thymidine kinase (htk) gene were introduced into mouse ltk- cells as direct repeats on a plasmid carrying a dominant selectable marker. following stabilization of these sequences in the recipient cell, selection for tk+ was applied to detect recombinational ... | 1983 | 6578876 |
| protection of mice from fatal herpes simplex virus type 1 infection by adoptive transfer of cloned virus-specific and h-2-restricted cytotoxic t lymphocytes. | a cloned culture of secondary anti-herpes simplex virus (anti-hsv) cytotoxic t lymphocytes (ctl) generated in vitro when adoptively transferred to intact or cyclophosphamide (cp) pretreated syngeneic mice protected the recipients from death following intraperitoneal infection with hsv-1. this in vivo protective effect conferred by anti-hsv ctl was virus-specific and h-2k/d-restricted. twenty-four h after hsv-1 infection of balb/c mice (intact or cp-pretreated) relatively high levels of serum int ... | 1983 | 6601180 |
| severe acquired immune deficiency syndrome in male homosexuals: diminished capacity to make interferon-alpha in vitro associated with severe opportunistic infections. | natural killer cell function, directed against either k562 tumor targets or herpes simplex virus type 1-infected fibroblasts, was often low in patients with acquired immune deficiency syndrome (aids) but failed to distinguish these patients from either male homosexual controls or patients with lymphadenopathy. mononuclear cells from patients with aids and opportunistic infections generated diminished levels of interferon-alpha in response to herpes simplex virus type 1-infected fibroblasts. this ... | 1983 | 6606691 |
| disseminated neonatal herpes simplex virus type 1 from a maternal breast lesion. | 1983 | 6828354 | |
| relapsing herpes simplex encephalitis following antiviral therapy. | although treatment for herpes simplex virus (hsv) encephalitis with antiviral agents has improved survival, occasional patients experience unexplained clinical exacerbations. this report presents evidence that some relapses may occur from recurrent viral encephalitis. an adult male developed the classic symptoms of hsv encephalitis. the cerebrospinal fluid (csf), electroencephalogram, and isotope brain scan suggested a localized encephalitis involving the left temporal lobe. the patient was trea ... | 1983 | 6830178 |
| differential susceptibility of human neural cell types in culture to infection with herpes simplex virus. | the ability of herpes simplex virus (hsv)-1 glasgow (strain 17), hsv-2 (strain hg52), temperature sensitive mutants and a thymidine kinase negative mutant of glasgow strain 17 to produce a cytopathic effect and induce viral antigen expression in primary and passaged human fetal brain cells was studied. no difference was detected between the behaviour of hsv-1 and hsv-2. cell-type-specific markers and indirect immunofluorescence were used to define unambiguously the type of individual cells in cu ... | 1983 | 6831193 |
| detection of type-specific antibody to herpes simplex virus type 1 by radioimmunoassay with herpes simplex virus type 1 glycoprotein c purified with monoclonal antibody. | herpes simplex virus type 1 (hsv-1) and hsv-2 specify at least four glycoproteins designated ga/gb, gc, gd, and ge. previous studies have shown that gc produced by hsv-1 is antigenically distinct from the corresponding hsv-2 glycoprotein. with the exception of gc, the glycoproteins of both serotypes share antigenic sites. standard serological assays fail to differentiate the antibody to the shared antigenic determinants from the type-specific antibody. in this paper, we describe a procedure for ... | 1983 | 6832831 |
| leptomeningitis and polycaryocyte formation in the cns of rats inoculated subcutaneously with herpes simplex virus. | wistar rats were inoculated subcutaneously with either type 1 (hsv1) or type 2 (hsv2) herpes simplex virus at 5 days of age. animals were killed in extremis or at the end of the 14-day observation period postinoculation. acute destructive meningoencephalitis with hemorrhage and leukocytic infiltration was observed in both groups. polycaryocytes comprised of cells of the internal granular layer of the cerebellum were observed in some animals inoculated with hsv1. these multinucleated cells appear ... | 1983 | 6852211 |
| efficacy of (e)-5-(2-bromovinyl)-2'-deoxyuridine in the treatment of experimental herpes simplex virus encephalitis in mice. | systemic treatment of mice with (e)-5-(2-bromovinyl)-2'-deoxyuridine (bvdu) showed a significant therapeutic efficacy against herpes simplex type 1 virus (hsv-1) encephalitis. with treatment initiated 12 h after viral inoculation and continued for 10 consecutive days, bvdu administered intraperitoneally in daily doses of 100-500 mg/kg increased the 21-day survival rates from 30 to 100% and reduced brain virus titers by 3-4 log10 on day 6 post-infection. furthermore, at doses of 300-500 mg/kg per ... | 1983 | 6870230 |
| viral antibodies in maternal and cord sera. | viral antibodies were determined in paired maternal and cord blood sera of 258 consecutive deliveries. antibodies to epstein-barr virus (ebv), cytomegalovirus (cmv), herpes simplex type 1 (hsv-1), adenovirus (adv), and varicella zoster (vzv) were tested by indirect immunofluorescence and to rubella (rub) by hemagglutination inhibition. analysis of the overall pattern of differences between maternal and cord blood showed a highly significant difference for all six viruses. analysis regarding indi ... | 1983 | 6877173 |
| structural requirements of olefinic 5-substituted deoxyuridines for antiherpes activity. | a number of structurally related 5-substituted pyrimidine 2'-deoxyribonucleosides were synthesized and tested for antiviral activity against herpes simplex virus type 1 (hsv-1) in cell culture. a minimum inhibitory concentration was determined for each compound, and from a comparison of these values a number of conclusions were drawn with regard to those molecular features that enhance or reduce antiviral activity. optimum inhibition of hsv-1 in cell culture occurred when the 5-substituent was u ... | 1983 | 6887201 |
| amino acid utilization by vero cells in microcarrier culture. | vero cells were grown in microcarrier culture and subsequently infected with herpes simplex virus (type 1). amino acid analyses were conducted on culture supernatants using a rapid reverse-phase high performance liquid chromatography method. the utilization of amino acids by the cells during cell growth and virus production phases was examined. during cell growth, glutamine and histidine were rapidly depleted (80-90%) by vero cells while arginine and tryptophan-menthionine (peaks not resolved) w ... | 1983 | 6329858 |
| rapid diagnosis of varicella-zoster virus infection by detection of viral deoxythymidine kinase in serum and vesicle fluid. | a sensitive enzyme assay utilizing [125i]iododeoxyuridine as the substrate and ctp as the phosphate donor in combination with isozyme-specific antisera was used for direct detection and typing of herpesvirus deoxythymidine kinase (dtk) in clinical specimens. an investigation of 16 coded vesicle fluid specimens, taken in connection with varicella-zoster virus (vzv) and herpes simplex virus infections, revealed viral dtk activity in 14 samples. all positive samples except one were taken within 5 d ... | 1983 | 6339548 |
| fate of microfilaments in vero cells infected with measles virus and herpes simplex virus type 1. | in herpes simplex virus type 1-infected vero cells, reorganization of microfilaments was observed approximately 4 h postinfection. conversion of f (filamentous) actin to g (globular) actin, as assessed by a dnase i inhibition assay, was continuous over the next 12 to 16 h, at which time a level of g actin of about twice that observed in uninfected cells was measured. fluorescent localization of f actin, using 7-nitrobenz-2-oxa-1,3-diazole (nbd)-phallacidin, demonstrated that microfilament fibers ... | 1983 | 6343843 |
| genital herpes - the disease and its treatment including intravenous acyclovir. | this paper firstly reviews the natural history of genital herpes, giving special attention to the differences between primary and non-primary initial disease and recurrent disease and between herpes simplex virus 1 and 2. secondly, previous therapies for genital herpes, and in particular the problems associated with previous drug trials are considered. the final part of the paper reviews a double-blind placebo controlled randomized study of intravenous acyclovir in patients with severe first att ... | 1983 | 6355051 |
| current status and prospects for oral acyclovir treatment of first episode and recurrent genital herpes simplex virus. | herpes simplex genitalis is an increasingly common primary or recurrent hsv infection. acyclovir (acv) has been found to diminish virus shedding and clinical symptoms in patients with first episode genital hsv when given topically or intravenously. as most patients with first episode disease do not require hospitalization and recurrent disease is usually less severe than the primary infection, out-patient treatment forms the basis of practical management. acyclovir is absorbed orally with plasma ... | 1983 | 6355052 |
| isolation and characterization of sequences from mouse chromosomal dna with ars function in yeasts. | fragments of chromosomal dna from a variety of eucaryotes can act as arss (autonomously replicating sequence) in yeasts. arss enable plasmids to be maintained in extrachromosomal form, presumably because they function as initiation sites for dna replication. we isolated eight different sequences from mouse chromosomal dna which function as arss in saccharomyces cerevisiae (bakers' yeast). although the replication efficiency of the different mouse arss in yeasts appears to vary widely, about one- ... | 1983 | 6361521 |
| ocular herpes simplex virus infection is diminished by depletion of b lymphocytes. | rabbit anti-mouse mu-chain serum was used to deplete specifically igm-bearing b cells and b cell function in balb/c mice. b cell-depleted and normal littermates were infected via the cornea with herpes simplex virus type 1 (hsv-1) at 4 to 5 wk of age. b cell-depleted mice had a reduced number of lymphocytes bearing surface immunoglobulin and a greatly reduced ability to produce antibody. mortality was reduced from 89% in normal controls to 42% in b cell-depleted mice (p less than 0.001) and peak ... | 1983 | 6411818 |
| hsv-1 shedding by lontophoresis of 6-hydroxydopamine followed by topical epinephrine. | the development of an animal model for herpes simplex virus (hsv) reactivation is of great importance in studying hsv latency, reactivation, recurrence, and chemotherapy. epinephrine iontophoresis to the cornea can induce hsv type 1 (hsv-1) ocular shedding from latently infected rabbits. in the present experiment, adrenergic induction of hsv-1 was enhanced by iontophoresis of 6-hydroxydopamine (6-hd) to the rabbit cornea followed by topical epinephrine to the eye. eye swabs were utilized to dete ... | 1983 | 6418684 |
| broad-spectrum antiviral activity of 2-beta-d-ribofuranosylselenazole-4-carboxamide, a new antiviral agent. | the relative in vitro antiviral activities of three related nucleoside carboxamides, ribavirin (1-beta-d-ribofuranosyl-1,2,4-triazole-3-carboxamide), tiazofurin (2-beta-d-ribofuranosylthiazole-4-carboxamide), and selenazole (2-beta-d-ribofuranosylselenazole-4-carboxamide), were studied against selected dna and rna viruses. although the activity of selenazole against different viruses varied, it was significantly more potent than ribavirin and tiazofurin against all tested representatives of the ... | 1983 | 6615611 |
| antibodies to herpes simplex type 1 in patients with active duodenal ulcer. | one hundred seventy-two patients requiring upper gastrointestinal tract endoscopy were examined prospectively for evidence of herpes simplex virus type 1 (hsv-1) infection. no viruses were recovered from active ulcers (11 with duodenal and eight with gastric ulcers). using both enzyme-linked immunosorbent assay (elisa) and standard complement fixation methods, patients with endoscopically proved active duodenal ulcer had significantly higher mean serum antibody levels to hsv-1 (but not to cytome ... | 1983 | 6625778 |
| the effects of dietary t-2 toxin on acute herpes simplex virus type 1 infection in mice. | young male white swiss mice were fed control diet or diet supplemented with 20 or 10 parts per million (ppm) of t-2 toxin for two or three weeks. these mice then were inoculated with herpes simplex virus type 1 (hsv-1) (9.6 x 10(6) plaque forming units) intraperitoneally. to compare the effects of t-2 toxin against a known immunosuppressive drug, cyclophosphamide was injected intraperitoneally at 150 mg/kg, 24 hours after treatment with hsv-1, into mice fed the control diet. mice were necropsied ... | 1983 | 6649341 |
| comparison of the efficacy of vidarabine, its carbocyclic analog (cyclaradine), and cyclaradine-5'-methoxyacetate in the treatment of herpes simplex virus type 1 encephalitis in mice. | the relative therapeutic effects of vidarabine (9-beta-d-arabinofuranosyladenine), cyclaradine (the adenosine deaminase-resistant carbocyclic analog of vidarabine), and cyclaradine-5'-methoxyacetate in the parenteral treatment of systemic herpes simplex virus type 1 infections in swiss mice were determined. among control mice inoculated intraperitoneally with virus, a mortality rate of 95% was observed. the intraperitoneal administration of nontoxic doses of vidarabine (125 to 250 mg/kg per day) ... | 1983 | 6651280 |
| the efficiency of genetic transformation of mammalian cells by transfection and microinjection depends on the transferred gene. | the efficiency of genetic transformation of mammalian cells was analysed with respect to the kind of the transferred gene and the selective system. plasmids pago and pag60 harboring the thymidine kinase gene of herpes simplex virus type 1 and the bacterial neomycin resistance gene, respectively, were compared concerning their ability to transform mouse ltk-aprt- cells. using the calcium phosphate technique the neomycin resistance gene transformed at least ten times more efficiently than the thym ... | 1983 | 6651803 |
| activity of amp against experimental herpes simplex virus type 1 infections in mice. | administration of amp soon after inoculation of mice with herpes simplex virus type 1 inhibited development of virus-induced lesions and appeared to prevent establishment of virus latency. these effects were dependent on both the amp dose and the time of amp administration. regression of herpes simplex virus type 1-induced lesions was also accelerated significantly by amp treatment in a time- and dose-dependent manner. | 1983 | 6660854 |
| inhibition of terminal deoxynucleotidyltransferase by (e)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate. | (e)-5-(2-bromovinyl)-2'-deoxyuridine 5'-triphosphate (bvdutp), known as a specific inhibitor of herpes simplex virus (type 1)-dna polymerase, was found to be a potent inhibitor of the activity of terminal deoxynucleotidyltransferase (tdt) from calf thymus. bvdutp was not an efficient substrate of tdt, but it inhibited the incorporation of normal deoxynucleotide substrates in competitive fashion at the nucleotide binding site of tdt molecule. the ki value for bvdutp (5 microm) was much less than ... | 1983 | 6664844 |
| [herpes simplex infection as a sexually transmitted disease]. | genital herpes simplex virus (hsv-)infection is a disease of growing importance in sexually transmitted diseases. it is the most common cause of genital ulcerations throughout the industrialized nations. it is caused by either herpes simplex virus type 1 or 2. genital herpes displays variable clinical manifestations in a primary or recurrent form. various seroepidemiologic studies suggest that asymptomatic infection is common. primary infection causes severe genital lesions and has a relatively ... | 1983 | 6689458 |
| fragments from both termini of the herpes simplex virus type 1 genome contain signals required for the encapsidation of viral dna. | a 535 base pair dna fragment which maps entirely within the irs/trs regions of the herpes simplex virus type 1 (hsv-1) genome and contains all the cis-acting signals necessary for it to function as an origin of viral dna replication has previously been identified (n.d. stow and e.c. mcmonagle, virology, in press). when bhk cells were transfected with circular plasmid molecules containing cloned copies of this dna fragment, and superinfected with wt hsv-1 as helper, amplification of the input pla ... | 1983 | 6324078 |
| [effect of ribamidil on the reproduction of the herpes simplex virus in a cv1 cell culture]. | cytotoxic properties and antiviral activity of abnormal nucleoside ribamidile were studied on the model of herpes simplex virus type 1 in cv1, continuous cell culture. the drug produced no cytotoxic effect even in concentrations of 500 and 250 micrograms/ml but had a marked capacity to inhibit herpes simplex virus reproduction in this system in concentrations of 125 and 62.5 micrograms/ml. antiviral effect of ribamidile was enhanced by its combined use with 5'-deoxy-5'-fluorothymidine. | 1983 | 6322451 |
| dna sequence of the herpes simplex virus type 2 glycoprotein d gene. | we describe a 1635-bp herpes simplex virus type 2 (hsv-2) dna sequence containing the entire coding region of glycoprotein d (gd-2). the amino acid sequence of gd-2, deduced from the nucleotide sequence, was compared to that of the analogous herpes simplex virus type 1 (hsv-1) glycoprotein (gd-1). the two glycoproteins are 85% homologous and contain highly conserved regions of as much as 49 amino acids in length. comparison of dna sequences upstream from gd-1 and gd-2 coding regions identified p ... | 1983 | 6323270 |
| [evaluation of 80 virologically-confirmed cases of ocular herpes. study of the correlation between the type of virus and the epidemiologic characteristics of the disease]. | 80 strains were isolated from patients with herpetic ocular infection during the period may 1979 to may 1981: 17 patients with herpetic blepharitis, 40 patients with superficial keratitis and 23 with deep stromal keratitis or kerato-uveitis. among 63 patients treated with antiviral agents, clinical resistance to the drug was observed in 17 cases. the strains were typed as hsv 1.1, 1.2 or 2.2 by seroneutralization and thermosensitivity in cellular cultures. no relationship between the viral types ... | 1983 | 6323567 |
| characterization of eukaryotic transcriptional control signals by assay of herpes simplex virus type 1 thymidine kinase. | we describe the characteristics of a general assay for eukaryote transcription-control sequences using the herpes simplex virus (hsv) thymidine kinase (tk) gene. after transfection of cultured cells with tk-containing recombinant plasmids, two assays were used to measure gene expression: short term or transient levels of tk mrna and tk enzyme activity, and the rate of biochemical transformation from a tk- to a tk+ phenotype in selective growth medium (hat). deletion of the endogenous tk promoter ... | 1983 | 6319553 |
| variable restriction endonuclease sites of herpes simplex virus type 1 isolates from encephalitic, facial and genital lesions and ganglia. | the distribution of restriction endonuclease (re) sites was compared for 84 herpes simplex virus type 1 (hsv-1) isolates obtained from the ganglia, facial lesions, genital lesions and from brain tissue from herpes encephalitis cases. the isolates came from canada, the u.k., the u.s.a. and japan. out of a total of 224 sites identified, 87 were variable. three of the 30 most variable sites were at significantly (p less than 0.05) different frequencies in groups of isolates from distinct anatomical ... | 1983 | 6319555 |
| glycoprotein c of herpes simplex virus type 1: characterization of o-linked oligosaccharides. | in contrast to other viral glycoproteins, the herpes simplex virus (hsv) glycoprotein c(gc) binds to the n-acetylgalactosamine-specific helix pomatia lectin (hpa). in the present paper gc was purified by affinity chromatography with monospecific antibodies and the purified glycoprotein was subjected to protease digestion. hpa-binding protease-resistant glycopeptides were isolated by lectin affinity chromatography. the isolated structures did not bind to concanavalin a and seemed to lack charged ... | 1983 | 6319556 |
| alterations in the recognition of nucleoside analogues as substrates by the deoxythymidine kinase of a 5-methoxymethyldeoxyuridine-resistant mutant of herpes simplex virus type 1. | inhibition constants (kis) were used as an estimate of the ability of various nucleoside analogues to be recognized as substrates by the deoxythymidine kinases (dtks) of a 5-methoxymethyldeoxyuridine-resistant (mmdur) mutant of herpes simplex virus type 1 (hsv-1) and its parent wild-type (wt). it was found that the kis for the 5-position analogues mmdu, [e]-5-(2-bromovinyl)deoxyuridine, bromodeoxyuridine and iododeoxyuridine were increased approximately three-to fivefold, suggesting that they we ... | 1983 | 6319559 |
| o-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the golgi apparatus. | the o-linked oligosaccharides on mature forms of herpes simplex virus type 1 (hsv1) glycoproteins were characterized, and were found to account largely for the lower electrophoretic mobilities of these forms relative to the mobilities of immature forms. other posttranslational modifications of hsv1 glycoproteins (designated gb, gc, gd and ge) were related temporally to the discrete shifts in electrophoretic mobilities that signal acquisition of the o-linked oligosaccharides. fatty acid acylation ... | 1983 | 6299584 |
| ribonucleotide reductase induced by herpes simplex virus has a virus-specified constituent. | ribonucleotide reductase, an enzyme found in all prokaryotic and eukaryotic cells that synthesize dna, is induced by herpes simplex virus (hsv). in this study the effect of anti-hsv antiserum on the induced ribonucleotide reductase has been examined and the ability of different temperature-sensitive (ts) mutants of hsv-1 to induce the enzyme has been investigated. the hsv-1-induced ribonucleotide reductase was inhibited by antiserum raised against infected cell lysates but not by preimmune serum ... | 1983 | 6298343 |
| characterization of abnormal thymidine kinases induced by drug-resistant strains of herpes simplex virus type 1. | two tk+ acyclovir-resistant variants of herpes simplex virus (hsv) (s1 and tr7) and one tk+ bvdu-resistant variant (b3) induce abnormal thymidine kinases with impaired ability to phosphorylate the drugs used in their isolation. these enzymes have been purified and their properties compared with those of the wild-type (wt) parent, sc16. the enzyme induced by s1 differed markedly from the other three in both its responses to salt and to ph. b3 tk recognized the enzyme's natural substrates, thymidi ... | 1983 | 6298344 |
| induction of a latent herpes simplex virus from a rat tumour initiated by herpes simplex virus-transformed cells. | a rat tumour induced by cells transformed with the sheared dna of herpes simplex virus (hsv) type 1 hfem alpha (re2a) was injected with the intertypic virus hsv-2 hg52 ts 1. separate plaques, isolated from cocultivation of excised tumour tissue with susceptible cells, yielded virus the dna of which had the restriction enzyme profile either of the injected hsv-2 virus or that of the hsv-1 virus, originally used to transform the cells. no evidence of in vivo recombination was detected. in hooded l ... | 1983 | 6298356 |
| expression of herpes simplex virus beta and gamma genes integrated in mammalian cells and their induction by an alpha gene product. | the proteins of herpes simplex virus type 1 (hsv-1) form three kinetic groups termed alpha, beta, and gamma, whose synthesis is regulated in a cascade fashion. alpha products are synthesized first during infection, and they are required for synthesis of beta and gamma proteins. to examine the expression of several hsv-1 beta and gamma genes in the absence of alpha functions, we transferred into mammalian cells a plasmid containing a region of the hsv-1 genome that codes for only beta and gamma g ... | 1983 | 6318078 |
| efficient transformation of dictyostelium discoideum amoebae. | we have transformed dictyostelium discoideum amoebae by using derivatives of a plasmid, pag60, which was designed for transformation of mammalian cells. the plasmid carries the promoter region of the herpes simplex virus type 1 thymidine kinase gene linked to the bacterial gene kan, which codes for the enzyme aminoglycoside 3'-phosphotransferase. kan is derived from the tn5 transposon. expression of the phosphotransferase permits direct selection of transformed cells by their resistance to the a ... | 1983 | 6318083 |
| sequential genital infections by herpes simplex viruses types 1 and 2: restriction nuclease analyses of viruses from recurrent infections. | virus isolated from a woman presenting with the first symptomatic episode of genital herpes was identified as herpes simplex virus type 1 (hsv-1) by restriction nuclease fingerprinting. testing for igm antibody to hsv indicated that the patient had recently contracted a new hsv infection. virus microneutralization and the micro-solid phase radioimmunometric test for igg, however, showed that the patient had had prior infection with herpes simplex virus type 2 (hsv-2); thus the hsv-1 infection wa ... | 1983 | 6318371 |
| identification and characterization of deoxyribonucleoprotein complexes containing the major dna-binding protein of herpes simplex virus type 1. | nuclear extracts were prepared from cells infected with herpes simplex virus type 1 (hsv-1) and fractionated by sucrose gradient centrifugation to identify deoxyribonucleoprotein complexes involved in viral replication. large amounts of an hsv-1 induced protein with a molecular weight of about 133,000 sedimented as a broad peak in the 25 s region of the gradient and cosedimented with 13 s dna fragments. the sedimentation of both the protein and dna decreased upon treatment of nuclear extracts wi ... | 1983 | 6318432 |
| poly(adp-ribosyl)ation of a herpes simplex virus immediate early polypeptide. | this paper reports in vitro poly(adp-ribosyl)ation of the herpes simplex virus type 1 (hsv-1) immediate early polypeptide vmw175. the phenomenon was most clearly observed by use of the temperature-sensitive mutant tsk, which overproduces vmw175 at the nonpermissive temperature (npt) and has a mutation in the coding sequences for this polypeptide. nuclei prepared from cells which were infected with tsk at npt and subsequently downshifted to the permissive temperature incorporated [32p]nad into vm ... | 1983 | 6318441 |
| lifestyle and antiviral antibody studies among homosexual men in denmark. | two-hundred and fifty-nine male homosexuals (hs) of a large and a small danish town were studied for antibodies to various virus and their statistical relationship to sex habits, drug use and other lifestyle factors. prevalence rates against cytomegalovirus (cmv) were 86.7% of hs men from copenhagen and 73.3% of hs men from the smaller community of aarhus, against 30% of a control group. antibody prevalence in homosexuals was significantly related to number of years of homosexual activity (p les ... | 1983 | 6318513 |
| kinetic analysis in cell culture of the reversal of antiherpes activity of nucleoside analogs by thymidine. | the inhibition of herpes simplex virus type 1 plaque formation by acyclovir, bromovinyldeoxyuridine, 9-(3,4-dihydroxybutyl)guanine, and 9-(4-hydroxybutyl)guanine at different thymidine concentrations was analyzed in lineweaver-burk plots. linear competitive patterns between thymidine and the nucleoside analogs were observed for the inhibition of herpes simplex virus type 1 plaque formation. a new constant, the reversal constant kr, was introduced to describe the sensitivity in cell culture of an ... | 1983 | 6318659 |
| neurovirulence of herpes simplex virus type 1 depends on age in mice and thymidine kinase expression. | the susceptibility of mice of different ages (from four to 28 days) to infection with herpes simplex virus type 1 (hsv-1) mutants inoculated onto scarified corneas was studied. the tk+ isolate from wild type virus was pathogenic in mice of all age groups. an hsv-1 mutant (designated tk1/4) with a less active thymidine kinase (tk) gene expressing 25 per cent of the tk activity of the tk+ isolate was pathogenic for mice up to 10 days of age. in older mice, virus pathogenicity was dependent on the ... | 1983 | 6318700 |
| recovery of herpes simplex virus 1 ts mutants from the dorsal root ganglia of mice. | 1983 | 6320265 | |
| [critical study of the serological diagnosis of herpetic encephalitis. apropos of 4 cases]. | virologic study was performed in four cases of herpes simplex virus encephalitis. herpes simplex virus type 1 was recovered from surgical brain biopsy specimens taken in three cases. anti-hsv antibodies were assayed in simultaneous blood and cerebrospinal fluid samples by elisa. csf and serum specific antibody ratios were compared to those of antibodies directed against other viral antigens whenever possible. a significant and early increase in this csf/serum ratio was demonstrated in two patien ... | 1983 | 6320376 |
| tumor-dependent resistance of rat peritoneal macrophages to herpes simplex virus. | by their position at sites of initial infection and their wide distribution in major organs of the body, macrophages may be decisive in determining the susceptibility or resistance of the host to virus infection. macrophage restriction of virus replication has been shown to be closely related to virus strains or virus types and to the age of the infected host. we report the effects of the development of a solid tumor in rats on intrinsic in vitro macrophage activity against herpes simplex virus ... | 1983 | 6299945 |
| frequency of cytotoxic t lymphocyte precursors to herpes simplex virus type 1 as determined by limiting dilution analysis. | the conditions for establishing a limiting dilution assay to measure cytotoxic t lymphocyte precursors (ctl-p) against herpes simplex virus type 1 (hsv-1) were determined. analysis by poisson statistics demonstrated that the estimated frequency of hsv-1-reactive cells in the spleens of normal mice was less than 1/250,000. in contrast, mice immunized previously with infectious hsv-1 demonstrated a ctl-p frequency between 1/3,500 and 1/15,670. the generation of a maximum cytotoxic t lymphocyte res ... | 1983 | 6299949 |
| latency of herpes simplex virus in ocular tissue of mice. | at 5 to 7 months after corneal inoculation of herpes simplex virus type 1 in mice, explants of ocular tissue yielded virus. immunoperoxidase study of explants undergoing reactivation revealed herpes simplex virus antigens in retinal tissue. these results indicate that herpes simplex virus can establish and maintain latency in ocular tissue, most probably in the retina. | 1983 | 6299954 |
| comparative neurovirulence of herpes simplex virus type 1 strains after peripheral or intracerebral inoculation of balb/c mice. | twenty-three strains of herpes simplex virus type 1 were compared for their pathogenicity in 4-week-old balb/c mice after peripheral (footpad) or intracerebral inoculation. among those strains examined were (i) six clinical isolates of brain or cerebrospinal fluid origin, (ii) seven clinical isolates of oral or genital origin, (iii) five prototype laboratory strains that have been passaged numerous times in culture, and (iv) five syncytial variants capable of producing cell fusion in culture. ba ... | 1983 | 6299955 |
| a comparative analysis of restriction enzyme digests of the dna of herpes simplex virus isolated from genital and facial lesions. | the restriction endonuclease (re) cleavage patterns of the dna of herpes simplex virus (hsv) genital isolates from two geographically distinct groups and from one group of facial isolates were examined. eleven of 21 genital isolates from females, 1 of 27 genital isolates from males and all 17 of the facial isolates were hsv type 1 (hsv-1). the groups of isolates of the same serotype could not be distinguished by significant differences in the frequency of variable restriction endonuclease sites ... | 1983 | 6300289 |
| herpes simplex virus type 1-induced interferon production and activation of natural killer cells in mice. | cultures of peritoneal exudate cells (pec) of non-immune c57bl/6 mice reacted to infectious herpes simplex virus type 1 (hsv), but not to non-infectious virions by producing interferon. similarly, when the virus preparations were injected intraperitoneally (i.p.) and interferon was determined in the wash-out fluid, interferon could only be detected after injection of infectious hsv. all interferons were mixtures of interferon-alpha and -beta. the cell that produced interferon was not sensitive t ... | 1983 | 6300294 |
| processing of herpes simplex virus type 1 glycoproteins: two-dimensional gel analysis using monoclonal antibodies. | the number of discrete species of glycoprotein induced by herpes simplex virus type 1 (hsv-1, strain 17 syn+) and their processing has been examined by a combination of immunoprecipitation with monoclonal antibodies and analysis of immune precipitates by two-dimensional (2d) gel electrophoresis. seventeen different monoclonal antibodies directed against glycoproteins a/b, c, d and e were used. polypeptides intermediate in the synthesis of ga/b, c and d were visualized and two early intermediates ... | 1983 | 6300307 |
| synthesis and biological activity of 5-(trifluoromethyl)- and 5-(pentafluoroethyl)pyrimidine nucleoside analogues. | various 5-substituted perfluoroalkylpyrimidine nucleoside analogues have been synthesized, and their biological activity against l1210, s-180, vero cells, and herpes simplex virus type 1 (hsv-1) was evaluated. the 5-trifluoromethyl derivatives, 7 and 9, showed significant antiviral activity against hsv-1 with ed50 values of 7 and 5 microm, respectively. in addition, the unblocked nucleoside 9 was found to be about 64-fold less toxic to the host vero cells and gave a favorable therapeutic index o ... | 1983 | 6300402 |
| synthesis and antiviral activity of water-soluble esters of acyclovir [9-[(2-hydroxyethoxy)methyl]guanine]. | several water-soluble ester derivatives of acyclovir [9-[(2-hydroxyethoxy)methyl]guanine], i.e., the 2'-o-glycyl-, 2'-o-alpha-alanyl-, 2'-o-beta-alanyl- and 2'-o-3-carboxypropionyl esters, were synthesized and evaluated for their antiviral activity in cell culture. the compounds were all prepared directly from acyclovir by application of the usual esterification methods with the appropriate acyl precursors and isolated as their hydrochloride or sodium salts. when assayed in primary rabbit kidney ... | 1983 | 6300403 |
| detailed analysis of the portion of the herpes simplex virus type 1 genome encoding glycoprotein c. | we previously showed that the right third of hindiii fragment l (0.59 to 0.65) of herpes simplex virus type 1 (hsv-1) encodes a family of mrnas some members of which appear to be related by splicing. in the experiments described in this communication, we determined the nucleotide sequence of the dna encoding this mrna family and precisely located the mrnas associated with this dna sequence. the major mrna species is unspliced and encoded by a 2.520-nucleotide region. just upstream of the 5' end ... | 1983 | 6300426 |
| identification and characterization of a herpes simplex virus gene product required for encapsidation of virus dna. | a mutant of herpes simplex virus type 1, 17tsvp1201, has a temperature-sensitive processing defect in a late virus polypeptide. immunoprecipitation studies with monoclonal antibodies showed that the aberrant polypeptide in mutant virus-infected cells was the nucleocapsid polypeptide known as p40. since a revertant, ts(+) for growth, processed the polypeptide normally under conditions restrictive for the mutant, the processing event must be essential for virus replication. electron microscopic an ... | 1983 | 6300447 |
| use of monoclonal antibodies against two 75,000-molecular-weight glycoproteins specified by herpes simplex virus type 2 in glycoprotein identification and gene mapping. | we produced two monoclonal antibodies that precipitate different glycoproteins of similar apparent molecular weight (70,000 to 80,000) from extracts of cells infected with herpes simplex virus type 2. evidence is presented that one of these glycoproteins is the previously characterized glycoprotein ge, whereas the other maps to a region of the herpes simplex virus type 2 genome collinear with the region in herpes simplex virus type 1 dna that encodes gc. | 1983 | 6300459 |
| analysis in cos-1 cells of processing and polyadenylation signals by using derivatives of the herpes simplex virus type 1 thymidine kinase gene. | bal31 nuclease was used to resect the herpes simplex virus type 1 thymidine kinase (tk) gene from its 3' end, and a plasmid, ptk206, was isolated that lacked the processing and polyadenylation signals normally found at the 3' end of the gene. the wild-type gene, ptk2, and ptk206 were each transferred to psv010, a plasmid containing the simian virus 40 (sv40) origin of dna replication, allowing replication and analysis of the patterns of transcription in cos-1 cells. fragments of dna containing p ... | 1983 | 6300661 |
| the study of dna-repair defects using [125i]iododeoxycytidine incorporation as an assay for the growth of herpes simplex virus. | [125i]iododeoxycytidine incorporation was used to measure herpes virus (hsv-1) dna synthesis following specific dna damage. xeroderma pigmentosum fibroblasts were less able to replicate uv-irradiated viral dna than were normal fibroblasts, indicating the necessity for excision repair for the survival of uv-irradiated virus. because of its rapidity and ease of quantitation, this assay had advantages over standard viral mediated assays of dna excision repair. it was possible to monitor viral repli ... | 1983 | 6300669 |
| detection of hsv-1 genome in central nervous system of latently infected mice. | herpes simplex virus type 1 (hsv-1) can establish latent infections in peripheral nerve ganglia and the central nervous system (cns) of experimentally infected mice. latent infections of peripheral nervous tissue are characterized by the ability to recover infectious virus from explant cultures of most of the latently infected ganglia. in contrast, infectious virus is infrequently recovered from the cns of latently infected mice following explant culture, although viral dna can be detected in cn ... | 1983 | 6300686 |
| molecular mimicry in virus infection: crossreaction of measles virus phosphoprotein or of herpes simplex virus protein with human intermediate filaments. | using monoclonal antibodies, we demonstrate that the phosphoprotein of measles virus and a protein of herpes simplex virus type 1 crossreact with an intermediate filament protein of human cells. this intermediate filament protein, probably vimentin, has a molecular weight of 52,000, whereas the molecular weights of the measles viral phosphoprotein and the herpes virus protein are 70,000 and 146,000, respectively. crossreactivity was shown by immunofluorescent staining of infected and uninfected ... | 1983 | 6300911 |
| the effect of ammonium chloride and tunicamycin on the glycoprotein content and infectivity of herpes simplex virus type 1. | infectious virions of mp, a syncytial strain of herpes simplex virus type 1, are formed in the presence of 50 mm nh4cl. underglycosylated virion glycoproteins are synthesized in infected cells and are incorporated into virions in the presence of the same concentration of nh4cl. we conclude that fully glycosylated glycoproteins are not required for viral infectivity. virus particles, deficient in glycosylated glycoproteins, are assembled in the presence of tunicamycin but they are not infectious. ... | 1983 | 6301148 |
| effect of acyclovir on viral protein synthesis in cells infected with herpes simplex virus type 1. | the effect of the antiviral agent 9-(2-hydroxyethoxymethyl)guanine (acyclovir) on herpes simplex virus type 1 protein synthesis during virus replication was examined. treatment of infected cells with acyclovir markedly affected the amounts of the four major glycosylated and certain non-glycosylated viral polypeptides synthesized; other viral polypeptides were made in normal amounts. the reduced amount of late protein synthesis was most likely due to the inhibition of progeny viral dna synthesis ... | 1983 | 6301368 |
| restriction of multiplication of herpes simplex virus type 1 in a myoepithelial cell line derived from human minor salivary gland. | 1983 | 6301408 | |
| neonatal herpes simplex virus infection in king county, washington. increasing incidence and epidemiologic correlates. | the incidence of neonatal herpes simplex virus (hsv) infection in king county, washington, has increased progressively from a rate of 2.6 per 100,000 live births to king county residents during the years 1966 through 1969 to 4.8, 7.4, and 11.9 cases per 100,000 live births for the years 1970 through 1973, 1974 through 1977, and 1978 through 1981, respectively. detailed interviews of the parents of newborns with neonatal hsv-2 infection revealed clinically symptomatic genital herpes in the mother ... | 1983 | 6315977 |
| experimental herpesvirus keratitis in the rabbit: topical versus intrastromal infection routes. | intrastromal and topical routes of infection of rabbit corneas with the hf strain of herpes simplex virus type 1 were compared clinically to determine which route of infection would present the best model of disciform edema and of deep stromal keratitis for our further studies on how the immune response of the infected animals may influence the expression of clinical disease. in addition, virus clearing and persistence of viral antigens as immunologic stimuli were monitored by virologic and elec ... | 1983 | 6316227 |
| characterization of the trs/irs origin of dna replication of herpes simplex virus type 1. | in previous experiments, an origin of viral dna replication was localized within a 995-bp dna fragment that mapped entirely within the trs/irs repeated region of the herpes simplex virus type 1 genome (n. d. stow, embo j. 1, 863-867, 1982). in this paper, this origin is now shown to reside within a 535-bp segment subcloned from the above fragment. deletions extending various distances into each end of the 535-bp segment were generated using nuclease bal 31, and the resulting plasmids screened fo ... | 1983 | 6316638 |
| physical location of a herpes simplex virus type-1 gene function(s) specifically associated with a 10 million-fold increase in hsv neurovirulence. | in this paper we present a technique developed to physically locate the hsv-1 gene(s) which restore neurovirulence to a non-neurovirulent hsv intertypic recombinant described in the preceding report. in brief, tissue culture cells are co-transfected with unit length re6 dna and restriction endonuclease fragmented hsv-1 (strain 17 syn+) dna. in this way, random recombinations between re6 and 17 syn+ are produced. an in vivo enrichment in mouse brains is then employed to select recombinants which ... | 1983 | 6316650 |
| processing of herpes simplex virus-1 glycans in cells defective in glycosyl transferases of the golgi system: relationship to cell fusion and virion egress. | we studied herpes simplex virus-1 (hsv-1) glycan structure and the expression of hsv-1 functions regulated by viral glycoproteins in ric21 cells (p. vischer and r. c. hughes, eur. j. bioch. 117, 275-284, 1981). this is a line of ricin-resistant mutant bhk cells defective in the enzymes of the golgi system which add terminal sugars to n-linked glycans. two kinds of alterations were observed in the glycosylation of hsv glycoproteins in ric21 cells. first, there was a defective processing of comple ... | 1983 | 6316656 |
| efficacy and selectivity of some nucleoside analogs as anti-human cytomegalovirus agents. | 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodocytosine (fiac), 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-methyluridine (fmau), 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-iodouridine (fiau), and 1-(2'-deoxy-2'-fluoro-beta-d-arabinofuranosyl)-5-ethyluridine (feau) were evaluated for antiviral activities against human cytomegalovirus (hcmv) and compared with 9-[(2-hydroxyethoxy)methyl]guanine (acyclovir) and e-5-(2'-bromovinyl)-2'-deoxyuridine (bvdu). the relative anti-hcmv pote ... | 1983 | 6316843 |
| antiherpes activity of (e)-5-(2-bromovinyl)- and 5-vinyl-1-beta-d-arabinofuranosyluracil and some other 5-substituted uracil arabinosyl nucleosides in two different cell lines. | of a series of 5-substituted 1-beta-d-arabinofuranosyluracil (5-x-arau) analogues, (e)-5-(2-bromovinyl)-arau(brvarau) and 5-vinyl-arau (varau) were the most potent inhibitors of plaque formation by two herpes simplex virus type 1 (hsv-1) strains in human embryonic lung fibroblast (helf) cell cultures. they were not only more active than 5-methyl-arau (marau, arat) and 5-ethyl-arau (earau), but even more than 1000 times more potent than the 5-fluoro, 5-iodo, 5-formyl and 5-trifluoromethyl (farau, ... | 1983 | 6316849 |