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description of a simple multiplex pcr-sscp method for ab0 genotyping and its application to the peopling of the canary islands.a simple and affordable multiplex polymerase chain reaction-single-strand conformation polymorphism method is proposed for the molecular study of ab0 polymorphisms. application of this method to the peopling of the canary islands, analyzing a total of 2,200 chromosomes, detected that in addition to berbers and basques, the rare alleles 0210 and o303 are also present in the iberian peninsula and in the canary islands. allele b101, with the highest frequency in northwest (nw) africa, shows a negat ...200516133448
erythema multiforme in a spotted hyena (crocuta crocuta).a 10.5-yr-old male spotted hyena (crocuta crocuta) developed acute, severe erythematous and ulcerative dermatitis of the hindquarters, feet, face, and ears 19 days after receiving a recombinant canary pox vectored canine distemper vaccine. biopsies of the lesions supported a diagnosis of erythema multiforme (em), which is an immune-mediated disorder that can occur days to months after exposure to antigenic stimuli. during the lengthy recovery, esophageal regurgitation and ulceration developed ei ...200517312775
an epizootic of avian pox in endemic short-toed larks (calandrella rufescens) and berthelot's pipits (anthus berthelotti) in the canary islands, spain.between january 2002 and november 2003, 50% (n = 395) of short-toed larks (calandrella rufescens) and 28% (n = 139) of berthelot's pipits (anthus berthelotti) examined on the islands of fuerteventura and lanzarote, canary islands, had gross lesions compatible with avian pox. however, spanish sparrows (passer hispaniolensis, n = 128) and trumpeter finches (bucanetes githagineus, n = 228), which inhabit the same steppe habitats associated with goat husbandry, did not have poxlike lesions. histopat ...200515657273
[socioeconomic inequalities and health plans in the autonomous communities of spain].to systematically examine sensitivity to socioeconomic (se) inequalities in the policies formulated in the health plans of the autonomous communities of spain.200516050962
outbreak of shigella sonnei in a rural hotel in la gomera, canary islands, spain.shigella sonnei is a significant cause of gastroenteritis in both developing and industrialized countries. knowledge of the diversity and antimicrobial susceptibility of the bacterium may be helpful in the management of both individual cases and outbreaks. this study was undertaken to evaluate the molecular epidemiology of an outbreak of diarrhea due to s. sonnei. the outbreak involved 14 of 28 (50%) tourists in a small rural hotel in la gomera, canary islands, spain. all of the s. sonnei isolat ...200516052462
colonisation and diversification of the blue tits (parus caeruleus teneriffae-group) in the canary islands.the blue tit (parus caeruleus teneriffae group) is proposed to have colonised the canary islands from north africa according to an east-to-west stepping stone model, and today, the species group is divided into four subspecies, differing in morphological, acoustic, and ecological characters. this colonisation hypothesis was tested and the population structure between and within the islands studied using mitochondrial dna sequences of the non-coding and relatively fast evolving control region. ou ...200515683925
genetic variation in epigenetic inheritance of ribosomal rna gene methylation in arabidopsis.we investigated the fidelity of epigenetic inheritance in crosses between three accessions of the flowering plant arabidopsis thaliana (canary islands, cape verde islands, and columbia). specifically, we examined the cytosine methylation content of the ribosomal rna genes at the two nucleolus organizer regions (nor2 and nor4) in f1 and f2 hybrid individuals derived from reciprocal crosses between the high nor methylation strain, columbia, and the two other accessions, both of which have less nor ...200515686517
nuclear ribosomal dna and karyotypes indicate a nw african origin of south american hypochaeris (asteraceae, cichorieae).hypochaeris has a disjunct distribution, with more than 15 species in the mediterranean region, the canary islands, europe, and asia, and more than 40 species in south america. previous studies have suggested that the new world taxa have evolved from ancestors similar to the central european h. maculata. based on internal transcribed spacer (its) sequences and fluorescence in situ hybridization (fish) with 5s and 18s-25s rdna of the previously overlooked hypochaeris angustifolia from moyen atlas ...200515737585
a fractal analysis of pathogen detection by biosensors.a fractal analysis is presented for the detection of pathogens such as franscisela tularensis, yersinia pestis (the bacterium that causes plague), bacillus anthracis, venezuelan equine encephalitis (vee) virus, vavcinia virus, and escherichia coli using a cellular analysis and notification of antigens risks and yields (canary) biosensor [t.h. rider, m.s. petrovic, f.e. nargi, j.d harper, e.d. schwoebel, r.h. mathews, d.j. blanchard, l.t bortolin, a.m. young, j. chen, m.a. hollis, a cell-based se ...200515617812
lack of effects of bacillus sphaericus (vectolex) on nontarget organisms in a mosquito-control program in southeastern wisconsin: a 3-year study.a 3-year study (2000-2002) in southeastern wisconsin was conducted to assess the effects of bacillus sphaericus applied for mosquito control on nontarget wetland invertebrates. the experimental design consisted of control and treatment sites (that were applied by helicopter with vectolex cg), each in 2 vegetation habitat types: reed canary grass marsh (phalaris arundinacea) and cattail marsh (typha spp.). in each of these areas, a predetermined number of timed (30-sec) d-frame aquatic net sample ...200516033123
bone cadmium and lead in the ancient population from el hierro, canary islands.this study was performed to determine the levels of lead (pb) and cadmium (cd) in 63 bone samples of the prehispanic population of the island el hierro, comparing them with the values obtained on 98 prehispanic samples from tenerife, fuerteventura, gran canaria, and la palma, all of them in the canary islands, and with eight modern samples who served as controls. prehispanic individuals from el hierro showed the lowest bone pb values of all the archipelago (0.72+/-1.01 mg/kg), significantly diff ...200516034152
heat transfer and the energetic cost of singing by canaries serinus canaria.sexually selected displays, such as male passerine bird song, are predicted to be costly. however, most measurements calculated the rate of oxygen consumption during singing using respirometry have shown that bird song has a low energetic cost. since birds are reluctant to sing when enclosed inside a respirometry chamber, the energetic cost of singing could differ from that under more normal circumstances. we used heat transfer modelling, based on thermal images, to estimate the energetic cost o ...200516034602
polycyclic aromatic hydrocarbons in smoke used to smoke cheese produced by the combustion of rock rose (cistus monspeliensis) and tree heather (erica arborea) wood.in this work, the polycyclic aromatic hydrocarbons (pahs) and their methyl derivatives concentrations have been determined in smoke from the rock rose and tree heather wood combustion. the combustion is done in two types of smokers, kiln and drum, commonly used in the canary islands (spain) to smoke cheese. the low control of the operational conditions justify the great variability of the pahs concentration in the emissions, with values between 251.8 and 2547 microg/m3n. in general, the lowest c ...200515631526
taenia spp.: 18s rdna microsatellites for molecular systematic diagnosis.the 18s rdna gene of adult worms of taenia parva found in genetta genetta in the iberian peninsula and larval stages of t. pisiformis from the wild rabbit (oryctolagus cuniculus) in tenerife (canary islands) were amplified and sequenced. the sequences of the 18s rdna gene of t. parva (1768 bp) and t. pisiformis (1760 bp) are reported for the first time (genbank accession nos. aj555167-aj555168 and aj555169-aj555170, respectively). in 168 alignment positions microsatellites in the 18s rdna of bot ...200515946391
the helminth fauna of the barbary partridge alectoris barbara in tenerife, canary islands.the helminth fauna of the barbary partridge (alectoris barbara) in tenerife island (canary archipelago) was studied from 2001 to 2002, as there were no records of helminths from this host in the canary islands. seven helminth species were identified: two cestodes choanotaenia infundibulum and lyruterina nigropunctata, and five nematodes aonchotheca caudinflata, baruscapillaria obsignata, eucoleus annulatus, ascaridia galli and heterakis gallinarum. lyruterina nigropunctata, a. galli and e. annul ...200515946395
[routine preoperative screening of asymptomatic patients: beliefs and attitudes of anesthesiologists in the canary islands, spain].to identify factors that explain anesthesiologists' inappropriate use of preoperative tests in asymptomatic patients scheduled for elective surgery.200515901024
an outbreak of abortions and high neonatal mortality associated with trypanosoma evansi infection in dromedary camels in the canary islands.trypanosoma evansi was diagnosed for the first time in the canary islands (spain) in 1998 in a dromedary camel. seroprevalences of 4.8% up to 9% have been observed using different diagnostic methods. affected animals have been treated but the dissemination of the disease is unknown. this article presents an outbreak of abortions and high neonatal mortality attributable to t. evansi infection in camels as well as the clinical assessment of the affected animals. the patients were diagnosed by rout ...200515893083
accumulating pollutants in conifer needles on an atlantic island - a case study with pinus canariensis on tenerife, canary islands.concentrations of potential pollutant elements na, cl, and s were investigated in needles of pinus canariensis grown at 55 field plots in tenerife. microelement concentrations (including heavy metals) were measured at a subset of 18 plots. na and cl concentrations were high at low elevations (up to 8 mg g(-1) cl and 5.5 mg g(-1) na). na/cl ratio close to standard seawater indicated sea spray influence up to 1200 m a.s.l. only at few plots, sulphur concentrations indicated possible pollutant impa ...200515862394
molecular characterization of a new serovar of salmonella bongori 13,22:z39:- isolated from a lizard.three salmonella strains isolated from a lizard (gallotia simoni) in the "isla del hierro" (canary islands, spain) were serotyped as salmonella bongori serotype 13,22:z39:-, which has not been described in the kauffmann-white scheme of salmonella serovars. in order to shed light on the assignment of those strains to the s. bongori species, several genes were amplified and/or sequenced. the irob gene has been reported to be present only in s. enterica, while the inva gene has been described as be ...200515862460
freedom and rules: the acquisition and reprogramming of a bird's learned song.canary song is hierarchically structured: short stereotyped syllables are repeated to form phrases, which in turn are arranged to form songs. this structure occurs even in the songs of young isolates, which suggests that innate rules govern canary song development. however, juveniles that had never heard normal song imitated abnormal synthetic songs with great accuracy, even when the tutor songs lacked phrasing. as the birds matured, imitated songs were reprogrammed to form typical canary phrasi ...200515890887
intraspecific comparative analysis of the species salinibacter ruber.salinibacter ruber is the first extremely halophilic member of the bacteria domain of proven environmental relevance in hypersaline brines at or approaching nacl saturation, that has been brought to pure culture. a collection of 17 strains isolated from five different geographical locations (mallorca, alicante, ebro delta, canary islands, and peruvian andes) were studied following the currently accepted taxonomic approach. additionally, random amplification of genomic dna led to the phenetic ana ...200515841344
mitochondrial dna diversity in 17th-18th century remains from tenerife (canary islands).mitochondrial dna sequences and restriction fragment length polymorphisms were retrieved (with >80% efficiency) from a 17th-18th century sample of 213 teeth from tenerife. the genetic composition of this population reveals an important ethnic heterogeneity. although the majority of detected haplotypes are of european origin, the high frequency of sub-saharan african haplotypes (15.63%), compared to that of the present-day population (6.6%), confirms the importance of the canary islands in the bl ...200515714457
a retrospective description of a highly pathogenic avian influenza a virus (h7n1/carduelis/germany/72) in a free-living siskin (carduelis spinus linnaeus, 1758) and its accidental transmission to yellow canaries (serinus canaria linnaeus, 1758).a haemagglutinating virus was isolated in summer 1972 from a single free-living siskin (carduelis spinus linnaeus, 1758) in embryonated chicken eggs. additional cases of morbidity or mortality were not observed in the area were the sick siskin was found. the virus was characterized as an avian influenza a virus of the subtype h7n1 and designated h7n1/carduelis/germany/72. the virus induced following experimental inoculation of chicken embryos a high rate mortality (mean death time approximately ...200515714871
isolation and identification of pathogenic acanthamoeba strains in tenerife, canary islands, spain from water sources.a comprehensive survey to document the presence of free-living amoebae of the genus acanthamoeba was conducted in tap water and sea water sources related to human environments in tenerife, canary islands, spain. acanthamoeba identification was based on the morphology of cyst and trophozoite forms and pcr amplification with a genus-specific primer pair. the pathogenic potential of acanthamoeba isolates was characterized by temperature and osmotolerance assays and pcr reactions with two primer pai ...200515678350
antimicrobial susceptibility of enterococcus strains isolated from poultry faeces.we have investigated the resistance of enterococcus isolated from poultry faeces to antibiotics commonly used as therapy of enterococcal infections. identification was made by the method of facklam and collins. minimal inhibitory concentrations of penicillin, ampicillin, vancomycin and teicoplanin were determined and high level aminoglycoside resistance was investigated. genes codifying high level aminoglycoside resistance (hlar) were determined by pcr. fifty five enterococcus strains were isola ...200515500837
inadvertent exposure to organochlorine pesticides ddt and derivatives in people from the canary islands (spain).in 1998, one of the largest determinations of organochlorine pesticides in a representative sample of a spanish population (682 serum samples from the canary islands) was made in the context of the "canary islands nutrition survey" (enca). in the canary islands, extensive farming areas have been developed in these last decades, with greenhouses dedicated to intensive cultivation using ddt in huge amounts. in spain, similarly to other european countries, ddt was banned in the late 1970s. the pest ...200515740757
bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (papilionoideae: genisteae) from the canary islands, along with bradyrhizobium japonicum bv. genistearum, bradyrhizobium genospecies alpha and bradyrhizobium genospecies beta.highly diverse bradyrhizobium strains nodulate genistoid legumes (brooms) in the canary islands, morocco, spain and the americas. phylogenetic analyses of its, atpd, glnii and reca sequences revealed that these isolates represent at least four distinct evolutionary lineages within the genus, namely bradyrhizobium japonicum and three unnamed genospecies. dna-dna hybridization experiments confirmed that one of the latter represents a new taxonomic species for which the name bradyrhizobium canarien ...200515774626
(gcg)11 founder mutation in the pabpn1 gene of opmd uruguayan families.the dominant oculo-pharyngeal muscular dystrophy mutation consists of an expanded (gcn)(12-17) in the coding region of the polya binding protein nuclear 1 gene. a founder effect has been demonstrated in canadian and bukhara jewish populations with relatively high prevalence of this disease. since the oculo-pharyngeal muscular dystrophy prevalence was remarkably high in southern uruguay, a founder effect was hypothesized. to identify the ancestral haplotype we determined the (gcn) repeat number a ...200515694141
smoking and sickness absence among public health workers.as well as causing sickness and death, smoking has high socio-economic costs. the aim of this paper was to examine how closely smoking is associated with sickness absence among healthcare workers.200515694961
morphology, cytochemical staining, and ultrastructural characteristics of the blood cells of the giant lizard of el hierro (gallotia simonyi).the object of this study was to examine the erythrocytes, leukocytes and thrombocytes of the giant lizard of el hierro (gallotia simonyi) by light and electron (tem) microscopy, and cytochemical staining. smears were prepared from blood from the ventral coccygeal vein of 10 healthy adult lizards (five males and five females) from the giant lizard of el hierro reproduction and research centre, canary islands, spain. the cytochemical stains used were: benzidine peroxidase (bp), chloroacetate ester ...200515563919
linguistic perception: neural processing of a whistled language.silbo gomero is a whistled language that is a rare and endangered surrogate of spanish, used by shepherds on the island of la gomera in the canary islands for communication over long distances on difficult terrain. here we show that areas of the brain normally associated with spoken-language function are also activated in proficient whistlers, but not in controls, when they are listening to silbo gomero. our findings demonstrate that the language-processing regions of the human brain can adapt t ...200515635400
y-chromosome str haplotypes in the canary islands population (spain).haplotype frequencies of eight y-chromosome str loci (dys19, dys389i, dys389ii, dys390, dys391, dys392, dys393 and dys385) were determined from a sample of 285 unrelated males from canary islands.200515639620
bahia grass pollen specific ige is common in seasonal rhinitis patients but has limited cross-reactivity with ryegrass.perennial ryegrass is a major cause of rhinitis in spring and early summer. bahia grass, paspalum notatum, flowers late into summer and could account for allergic rhinitis at this time. we determined the frequency of serum immunoglobulin (ig)e reactivity with bahia grass in ryegrass pollen allergic patients and investigated ige cross-reactivity between bahia and ryegrass.200515647049
measles virus genotype circulation in spain after implementation of the national measles elimination plan 2001-2003.molecular characterization of measles virus is important for disease surveillance and for monitoring elimination of the virus throughout the world. furthermore, knowledge of genotype distribution in as many countries as possible, is useful for tracing the origin of a strain, especially in countries without endemic measles disease, where most cases are imported. data on genotypes circulating in spain from 1970 to 1997 showed the prevalence of genotypes c1, c2, and d6, with subsequent replacement ...200515543577
prevalence and incidence of multiple sclerosis in las palmas, canary islands, spain.to determine the prevalence and incidence of multiple sclerosis (ms) in the city of las palmas (canary islands, spain), geographically belonging to north-western africa, but with european ancestry.200515459512
detour behaviour in three species of birds: quails (coturnix sp.), herring gulls (larus cachinnans) and canaries (serinus canaria).detour behaviour is the ability of an animal to reach a goal stimulus by moving round any interposed obstacle. it has been widely studied and has been proposed as a test of insight learning in several species of mammals, but few data are available in birds. a comparative study in three species of birds, belonging to different eco-ethological niches, allows a better understanding of the cognitive mechanism of such detour behaviour. young quails (coturnix sp.), herring gulls (larus cachinnans) and ...200515449104
molecular phylogenetics of limonium and related genera (plumbaginaceae): biogeographical and systematic implications.phylogenetic relationships within limonium (plumbaginaceae) are evaluated using sequence data from three plastid regions (rbcl, the trnl intron, and the trnl-trnf intergenic spacer). sixty-six species representing the major genera of staticoideae, including representatives of all sections and genera formerly included in limonium, have been analyzed using four species of plumbaginoideae as an outgroup. analyses of each separate and combined data set yield similar results. afrolimon is embedded in ...200521646141
patterns of genetic divergence of three canarian endemic lotus (fabaceae): implications for the conservation of the endangered l. kunkelii.we examined data for 11 allozyme loci in 14 populations that represent the distribution of the endangered lotus kunkelii, the narrowly distributed l. arinagensis (both endemic to gran canaria), and the broad-ranging l. lancerottensis (endemic to the easternmost canary islands, fuerteventura and lanzarote) to explore and construe patterns of genetic variation and use this data to assess the controversial taxonomic status of l. kunkelii relative to l. lancerottensis. while l. kunkelii maintains lo ...200621642177
the utility of automated analysis of inter-simple sequence repeat (issr) loci for resolving relationships in the canary island species of tolpis (asteraceae).plants of oceanic islands, often remarkably divergent morphologically from continental relatives, are useful models for studying evolution and speciation because evolution is telescoped in time and space. prior studies revealed little dna sequence variation within the clade of ca. 10 canary island species of tolpis, which precluded resolving species relationships. the present study assessed the utility of automated analysis of inter-simple sequence repeat (issr) loci for resolving relationships ...200621642181
population divergence for heteroblasty in the canary island pine (pinus canariensis, pinaceae).a heteroblastic (or vegetative phase) change is an abrupt manifestation in the general heteroblastic development during the ontogeny of plants. the canary island pine undergoes an especially marked and delayed heteroblastic change, including both the formation of secondary needles on dwarf shoots and the onset of preformed growth. to assess genetic and environmental effects on the heteroblastic change in this species, we followed plants from 19 populations at a dry site and a wetter site. compar ...200621642146
genetic structure of the population of pepino mosaic virus infecting tomato crops in spain.abstract the population structure of pepino mosaic virus (pepmv), which has caused severe epidemics in tomato in spain since 2000, was analyzed. isolates were characterized by the nucleotide sequence of the triple gene block and coat protein gene and, for a subset of isolates, a part of the rna-dependent rna polymerase gene. the full-length sequence of the genomic rna of a solanum muricatum isolate from peru also was determined. in spite of high symptom diversity, the spanish population of pepmv ...200618944442
allozyme diversity within and divergence among species oftolpis(asteraceae-lactuceae) in the canary islands: systematic, evolutionary, and biogeographical implications.plants endemic to oceanic islands represent some of the most unusual and rare taxa in the world. enzyme electrophoresis was used to assess genetic diversity within and divergence among all endemic species of a small genus of plants on the canary islands. our results show that the genus tolpis is similar to many other island groups in having generally low allozyme divergence among species, with the highest divergence found among four groups of endemics. the two rare and highly localized species t ...200621646225
reproductive biology of the dioecious canary islands endemic withania aristata (solanaceae).we confirmed functional dioecy of withania aristata via field and greenhouse studies. male flowers are significantly larger. female flowers bear stamens with no pollen; males bear 220?000 grains. stigmata of male flowers senesce in buds. anatomical observations confirm more ovules in females and an ovarian nectary in both sexes. we detected nectar in female flowers in the greenhouse but found no nectar in males. thus, males offer pollen and females nectar. females bear large numbers of fruits an ...200621642194
dynamical systems techniques reveal the sexual dimorphic nature of motor patterns in birdsong.in this work we analyze the pressure motor patterns used by canaries (serinus canaria) during song, both in the cases of males and testosterone treated females. we found a qualitative difference between them which was not obvious from the acoustical features of the uttered songs. we also show the diversity of patterns, both for males and females, to be consistent with a recently proposed model for the dynamics of the oscine respiratory system. the model not only allows us to reproduce qualitativ ...200617155106
characterization of estrogen receptors alpha and beta in uterine leiomyoma cells.cellular and subcellular localization of estrogen receptor alpha (eralpha) and estrogen receptor beta (erbeta) in uterine leiomyomas.200617011556
increasing incidence of megabacteriosis in canaries (serinus canarius domesticus).a total of 312 post-mortem examinations of 178 canaries (serinus canarius domesticus), 40 parakeets (melopsittacus undulatus, nymphicus hollandicus) and 94 parrots (amazona aestiva, psitaccus erithacus) were conducted at the birds and rabbits service of the university of liège, belgium. after a detailed gross examination, tissue samples were collected for virological and/or bacteriological and/or parasitological examination to complete the diagnosis. in all cases, a microscopic examination of th ...200616140025
genetic diversity and historical demography of atlantic bigeye tuna (thunnus obesus).bigeye (thunnus obesus) is a large, pelagic, and migratory species of tuna that inhabits tropical and temperate marine waters worldwide. previous studies based on mitochondrial rflp data have shown that bigeye tunas from the atlantic ocean are the most interesting from a genetic point of view. two highly divergent mitochondrial haplotype clades (i and ii) coexist in the atlantic ocean. one is almost exclusive of the atlantic ocean whereas the other is also found in the indo-pacific ocean. bigeye ...200616188460
presentation and role of transplantation in adult patients with type 1 primary hyperoxaluria and the i244t agxt mutation: single-center experience.primary hyperoxaluria type 1 (ph1) is a rare genetic disorder characterized by allelic and clinical heterogeneity. we aim to describe the presentation and full single-center experience of the management of ph1 patients bearing the mutation described in our community (i244t mutation+polymorphism p11l). since 1983, 12 patients with recurrent renal lithiasis have been diagnosed with ph1 and renal failure in the canary islands, spain. diagnostic confirmation was based on the presence of oxalosis in ...200616912707
pet therapy and institutionalized elderly: a study on 144 cognitively unimpaired subjects.the aim of this study was to assess whether a pet therapy program had a favorable effect on psychopathological status and perception of quality of life in cognitively unimpaired institutionalized elderly. seven elderly rest homes in veneto region of northern italy participated in the project, which was conducted on 144 cognitively intact elderly residents (97 females and 47 males). the participants were randomly divided into three groups: 48 subjects were given a canary, 43 subjects were given a ...200616191447
script-like attachment representations and behavior in families and across cultures: studies of parental secure base narratives.the articles included in this special issue of attachment and human development were originally presented as contributions to symposia at the society for research in child development (atlanta, georgia, april 2005) and at the european developmental psychology conference (laguna, canary islands, august 2005). the articles represent efforts of independent research teams studying the emergence, maintenance, and implications of attachment representations. in each study, a central measure of attachme ...200616938701
circovirus inclusion bodies in intestinal muscle cells of a canary.multiple cytoplasmic inclusion bodies were observed in the intestinal smooth muscle cells of an adult canary from an aviary with a history of high mortality (50%) both in adult and young birds. grossly, a mild enteritis was the only lesion appreciable. smears of the proventricular contents contained a few megabacteria (macrorhabdus ornithogaster). the intestinal inclusions were found in very high numbers in all parts of the tract examined. they appeared round to oval, amphophilic and hyaline in ...200616854639
seroprevalence of canine heartworm disease (dirofilaria immitis) on tenerife island: an epidemiological update.blood samples from 823 dogs were tested for circulating dirofilaria immitis antigen during a 1-year period (may 2002 to may 2003) on tenerife island, canary islands, spain. seroprevalence of heartworm infection was 21%. heartworm infection was similar in males and females and was more common in dogs aged >6 years. distribution of infection in varying climatic zones was not statistically different.200616821033
explaining sex differences in dental caries prevalence: saliva, hormones, and "life-history" etiologies.when dental caries rates are reported by sex, females are typically found to exhibit higher prevalence rates than males. this finding is generally true for diverse cultures with different subsistence systems and for a wide range of chronological periods. exceptions exist, but are not common. in this paper, we present new data for sex differences in dental caries rates among the guanches (tenerife, canary islands), summarize results of meta-analyses of dental caries prevalence, and emphasize new ...200616788889
[coronary risk in the population of the canary islands, spain, using the framingham function].although ischemic cardiopathy mortality in the canary islands is among the highest in spain, the specific coronary risk for its population has not been estimated. this study presents the first cardiovascular risk charts for the canarian adult population and compares them with those previously published on gerona, spain.200616756902
[christopher columbus flu. a hypothesis for an ecological catastrophe].when christopher columbus and his men embarked on the second colombian expedition to the new world (1493), the crew suffered from fever, respiratory symptoms and malaise. it is generally accepted that the disease was influenza. pigs, horses and hens acquired in gomera (canary islands) traveled in the same ship. the pigs may well have been the origin of the flu and the intermediary hosts for genetic recombination of other viral subtypes. the caribbean archipelago had a large population of birds, ...200616762260
genome of a novel circovirus of starlings, amplified by multiply primed rolling-circle amplification.the genus circovirus comprises small non-enveloped viruses with a circular single-stranded dna genome. by using pcr with degenerate primers, a novel circovirus (starling circovirus, stcv) was detected in spleen samples of wild starlings (sturnus vulgaris and sturnus unicolor) found dead during an epidemic outbreak of septicaemic salmonellosis in northeastern spain. using a specific pcr, stcv was also detected in apparently healthy birds from the same population. the genome was amplified using mu ...200616603520
antigenic and genetic characterization of an avian poxvirus isolated from an endangered hawaiian goose (branta sandvicensis).an avian poxvirus from cutaneous lesions in a hawaiian goose (branta sandvicensis) was characterized in this study. the virus was isolated by inoculation onto the chorioallantoic membranes (cams) of developing chicken embryos. cytoplasmic inclusion bodies were observed on histopathological examination of cam lesions. western blotting analysis using polyclonal antiserum against fowl poxvirus (fwpv) showed differences from fwpv, but a similar antigenic profile between hawaiian goosepox (hgp) isola ...200616617975
population genetics of ochlerotatus eatoni (diptera: culicidae) endemic species to two macaronesian islands.analyses of 11 isoenzyme loci of ochlerotatus eatoni (edwards, 1916), endemic to two macaronesian islands (madeira and tenerife, canary islands), revealed substantial genetic structure in the study populations. samples from sites on the south and north of madeira displayed a significant reduction of variability compared with those from central madeira and tenerife. the tenerife population exhibited a severe deficit of heterozygosity with similar magnitude across all the loci examined. the comple ...200616619604
mutation g47r in the bsnd gene causes bartter syndrome with deafness in two spanish families.bartter syndrome (bs) is a heterogeneous group of autosomal recessive hypokalaemic salt-losing tubulopathies. five types of bs caused by different genetic defects have been identified, and one of them is associated with sensorineural deafness (bsnd). mutations in the recently described bsnd gene, mapped in chromosome 1p31, have been reported to be associated with bsnd. this gene encodes barttin, an essential beta-subunit subunit for clc-ka and clc-kb channels. both subunits are co-expressed in b ...200616572343
increasing serum levels of non-ddt-derivative organochlorine pesticides in the younger population of the canary islands (spain).organochlorine pesticides are a lipophilic class of chemicals that persist in the environment and tend to accumulate in human tissues for years. they came into widespread use in the late 1940s. because of their capacity to bioaccumulate and biomagnify in food chains and their toxic effects, most of them were banned in industrialized countries, among them spain, in the late 1970s and 1980s. in 1998 organochlorine pesticides were determined in a representative sample of a spanish population (aroun ...200616580052
a souvenir from the canary islands. 200616581412
identification of a novel circovirus in australian ravens (corvus coronoides) with feather disease.the complete genome of a novel circovirus isolated from an australian raven (corvus coronoides) with feather lesions similar to those that occur in psittacine beak and feather disease is reported. degenerate polymerase chain reaction primers were designed to amplify and sequence novel circovirus dna from affected feathers. sequence analysis indicated that the tentatively named raven circovirus (racv) was 1898 nucleotides in size with two major open reading frames synonymous with other avian circ ...200616595298
new founding mutation in msh2 associated with hereditary nonpolyposis colorectal cancer syndrome on the island of tenerife.lynch syndrome or hereditary nonpolyposis colorectal cancer (hnpcc) is a hereditary syndrome with genetic heterogeneity. the disease is caused by mutations or epigenetic silencing in dna mismatch repair genes, mlh1, msh2, msh6, pms2 and mlh3, although the vast majority of cases correspond to mutations of mlh1 and msh2. we herein describe a nucleotide change, c.2063t>g in exon 13 of the msh2 gene, present in families that fulfill the amsterdam criteria for lynch syndrome and originate from northe ...200616500024
olive oil consumption and risk of breast cancer in the canary islands: a population-based case-control study.breast cancer mortality and incidence rates in the canary islands, and particularly in gran canaria, are higher than those in the rest of spain.200616512965
renal replacement therapy in the canary islands: demographic and survival analysis.the continuous increase in the number of patients on renal replacement therapy (rrt) has heightened the importance of renal patient registries to respond to the demand for data on the state of health, quality and cost of care provided for these patients. our aim was to analyze the epidemiological profile of this population in the canary islands.200616523433
testing phylogeographic predictions on an active volcanic island: brachyderes rugatus (coleoptera: curculionidae) on la palma (canary islands).volcanic islands with well-characterized geological histories can provide ideal templates for generating and testing phylogeographic predictions. many studies have sought to utilize these to investigate patterns of colonization and speciation within groups of closely related species across a number of islands. here we focus attention within a single volcanic island with a well-characterized geological history to develop and test phylogeographic predictions. we develop phylogeographic predictions ...200616448412
[diabetes mellitus in spain: death rates, prevalence, impact, costs and inequalities].describing the situation of diabetes mellitus (dm) in spain from a public health perspective. material and method: manual review of books and other documents on diabetes mellitus in spain was conducted. in addition, a specific research of articles published using mesh terms diabetes mortality, prevalence, incidence, cost, inequalities and spain was conducted in medline through internet (pubmed). minimun basic data set was utilized as source for complication description by communities autonomus.200616539961
evaluation of 5 new media containing extracts of seeds applied to candida dubliniensis screening.candida dubliniensis is a recently described pathogenic species that shares many phenotypic features with candida albicans and so may be misidentified in microbiologic laboratories. the aim of this study is to find a useful and cost-effective method suitable for screening c. dubliniensis before proceeding to further identification. we examined the colony morphology and chlamydospore production of 26 c. dubliniensis isolates and 100 c. albicans isolates on the following 5 proposed media: sesame s ...200616545931
qualitative inheritance of rind pattern and flesh color in watermelon.watermelon [citrullus lanatus (thunb.) matsum. & nakai var. lanatus] is a diverse species, with fruits of different sizes, shapes, rind patterns, and flesh colors. this study measured the inheritance of novel rind phenotypes and verified the genetics of white, red, salmon yellow, and canary yellow flesh colors. for each of the 11 crosses, six generations (p(a)s1, p(b)s1, f1, f2, bc1p(a), and bc1p(b)) were produced to form 11 families. three new genes were identified and designated as follows: sc ...200616489140
analysis by gas chromatography-mass spectrometry of the volatiles from the fruits of ammodaucus leucotrichus subsp. leucotrichus and subsp. nanocarpus grown in north africa and the canary islands, respectively.the volatiles from the fruits of ammodaucus leucotrichus subsp. leucotrichus and subsp. nanocarpus (two endemic species, the first from north africa and the second from the canary islands, spain) were studied by gas chromatography and gas chromatography-mass spectrometry. the major components of the volatiles of subsp. nanocarpus were found to be, beta-pinene (22.2-33.6%), bornyl angelate (20.6-21.8%) and camphor (8.3-11.7%) whereas in the fruits of subsp. leucotrichus, the main constituents wer ...200616472529
the questionable contribution of the neolithic and the bronze age to european craniofacial form.many human craniofacial dimensions are largely of neutral adaptive significance, and an analysis of their variation can serve as an indication of the extent to which any given population is genetically related to or differs from any other. when 24 craniofacial measurements of a series of human populations are used to generate neighbor-joining dendrograms, it is no surprise that all modern european groups, ranging all of the way from scandinavia to eastern europe and throughout the mediterranean ...200616371462
raillietiella morenoi sp. n. (pentastomida) from gallotia atlantica (peters and doria, 1882) (lacertidae) in the canary islands.raillietiella morenoi sp. n., a new cephalobaenid pentastomid found in the lungs of gallotia atlantica collected in the canary islands, is described. the new species belongs to the sharp-tipped posterior-hook type. the annulus number, morphology, and dimensions of copulatory spicules and the dimensions of anterior and posterior hooks separate raillietiella sp. n. from the other raillietiedid sharp-tipped posterior-hook species of small lizard parasites in africa. the host character of endemic pr ...200616380839
enhanced viral and tumor immunity with intranodal injection of canary pox viruses expressing the melanoma antigen, gp100.the route of administration and extent of helper t-cell activation are factors that are likely to be important for the development of effective cancer vaccines. in order to optimize cd8(+) cytotoxic t-lymphocyte (ctl) responses, the immunologic effects of direct lymph node (ln) injections of canary pox virus (alvac) vectors (expressing the melanoma antigen, gp100) and immunogenic gp100 peptides, along with concomitant injections of the helper adjuvant, tetanus toxoid, were studied in high-risk h ...200616404742
cadmium dietary intake in the canary islands, spain.cadmium (cd) in the human diet constitutes a potential chronic hazard to health. in the nonsmoking general population, diet is the major source of cadmium exposure; therefore, it is important to monitor the dietary intake of this heavy metal to quantify and improve the understanding of cd accumulation in the human body. the purpose of this study was to determine the levels of cd in a range of food and drink commonly consumed in the canary islands. food samples (420) were analyzed for cd by atomi ...200616337849
auditory topography and temporal response dynamics of canary caudal telencephalon.to map the encoding of auditory cues in songbirds, multiunit electrophysiological responses to pure tone stimuli (250-5000 hz) were recorded at 373 sites throughout the avian analogue of the mammalian auditory cortex in the caudal telencephalon of awake, restrained canaries. we found that a dorso-ventral tonotopic gradient from low to high frequency stimuli extends from the rostral field l2 to caudal-most caudo-medial nidopallium (ncm), similar to the frequency-dependent patterns of zenk gene ex ...200616329130
mediterranean diet and metabolic syndrome: a cross-sectional study in the canary islands.assessment of relation between metabolic syndrome (ms) and mediterranean diet (md) adherence.200617378946
nonlinear model predicts diverse respiratory patterns of birdsong.a central aspect of the motor control of birdsong production is the capacity to generate diverse respiratory rhythms, which determine the coarse temporal pattern of song. the neural mechanisms that underlie this diversity of respiratory gestures and the resulting acoustic syllables are largely unknown. we show that the respiratory patterns of the highly complex and variable temporal organization of song in the canary (serinus canaria) can be generated as solutions of a simple model describing th ...200616486997
three times out of asia minor: the phylogeography of arabis alpina l. (brassicaceae).arabis alpina is a characteristic plant in arctic-alpine habitats and serves as a classical example to demonstrate biology, ecology and biogeography of arctic-alpine disjuncts. it has a wider distribution than most other arctic-alpine plants, covering all european mountain systems, the canary islands, north africa, the high mountains of east africa and ethiopia, the arabian peninsula and mountain ranges of central asia in iran and iraq. additionally it is found in the northern amphi-atlantic are ...200616499705
social context affects testosterone-induced singing and the volume of song control nuclei in male canaries (serinus canaria).the contribution of social factors to seasonal plasticity in singing behavior and forebrain nuclei controlling song, and their interplay with gonadal steroid hormones are still poorly understood. in many songbird species, testosterone (t) enhances singing behavior but elevated plasma t concentrations are not absolutely required for singing to occur. singing is generally produced either to defend a territory or to attract a mate and it is therefore not surprising that singing rate can be influenc ...200616838373
historical population dynamics and demography of the eastern atlantic pomacentrid chromis limbata (valenciennes, 1833).recent studies have focused on the relationship between the marine fauna of the eastern atlantic and the mediterranean sea, but within the atlantic, little is known about genetic relationships between populations of the macaronesian islands. in this study, we tested whether the paleo-climatology and paleo-oceanography of the region could predict the genetic relationships among three eastern atlantic populations (azores, madeira, and canaries) of a damselfish, chromis limbata, and compared our re ...200616603388
respiratory patterns in oscine birds during normal respiration and song production.in this work we study the generation of respiratory patterns by oscine birds. we present a model capable of generating realistic respiratory patterns, during normal respiration and song production. the model accounts for the interaction between neural nuclei and air sac dynamics. we performed experiments in vivo in order to test the predictions of the model, measuring air sac pressure during song and normal respiration in canaries (serinus canaria).200616906868
molecular evidence for adaptive radiation of micromeria benth. (lamiaceae) on the canary islands as inferred from chloroplast and nuclear dna sequences and issr fingerprint data.the canary islands have been a focus for phylogeographic studies on the colonization and diversification of endemic angiosperm taxa. based on phylogeographic patterns, both inter island colonization and adaptive radiation seem to be the driving forces for speciation in most taxa. here, we investigated the diversification of micromeria on the canary islands and madeira at the inter- and infraspecific level using inter simple sequence repeat pcr (issr), the trnk-intron and the trnt-trnl-spacer of ...200616839782
chloroplast microsatellites reveal colonization and metapopulation dynamics in the canary island pine.chloroplast microsatellites are becoming increasingly popular markers for population genetic studies in plants, but there has been little focus on their potential for demographic inference. in this work the utility of chloroplast microsatellites for the study of population expansions was explored. first, we investigated the power of mismatch distribution analysis and the f(s) test with coalescent simulations of different demographic scenarios. we then applied these methods to empirical data obta ...200616911194
apparent 'sympatric' speciation in ecologically similar herbivorous beetles facilitated by multiple colonizations of an island.coexistence of recently diverged and ecologically similar sister species in complete sympatry represents a particularly compelling case for sympatric speciation. this study investigates the possible sympatric origin of two coexisting bark beetle taxa that utilize the same host plant on the island of la palma in the canary islands. aphanarthrum subglabrum and aphanarthrum glabrum ssp. nudum breed inside dead twigs of euphorbia lamarckii plants and are closely related to the allopatric a. glabrum ...200616911212
optimization of an analytical methodology for the determination of alkyl- and methoxy-phenolic compounds by hs-spme in biomass smoke.a sampling and analysis method for the determination of 21 phenolic compounds in smoke samples from biomass combustion has been developed. the smoke is used to make smoked foods, following an artisanal procedure used in some parts of the canary islands. the sampling system consists of a bravo h air sampler, two impingers, each one containing an aqueous solution of sodium hydroxide 0.1 mol l(-1), followed by a silica gel trap. the variables optimized to reach the best sampling conditions were vol ...200616710695
the "canary incident" in w.c. field's 1928 earl carroll's vanities stage dental skit.w.c. fields (1880-1946), a gifted writer, director and performer, became one of the greatest comedians, jugglers and pantomimes of all time. this crusty, opinionated curmudgeon, whose multifaceted career spanned most of the 20th century, made his name in burlesque, vaudeville, the legitimate stage, silent pictures, talkies, radio, books and recordings. 1-3 he died before a new media -- television - could have brought him even more success. the following article explores a fascinating incident wh ...200616764237
the canary in the coal mine. 200616789679
randomized clinical trial of telepsychiatry through videoconference versus face-to-face conventional psychiatric treatment.although telepsychiatry in the form of videoconferencing has been well received in terms of increasing access to care and user satisfaction, few data on treatment outcomes and efficacy from telepsychiatry applications are available at the present time. this paper evaluates the efficacy of telepsychiatry through videoconference in the treatment of mental disorders by comparing to face-to-face conventional (f2fc) treatment. we carried out a randomized clinical trial where 140 psychiatric outpatien ...200616796502
gathered mediterranean food plants--ethnobotanical investigations and historical development.the diversity of local mediterranean food elements is not known in detail, but offers itself to search for new vegetables, salads, fruits and spices which could be used in to enrich diets outside their region of origin. most amid those interesting local elements are edible wild plants and weeds. ethnobotanical research has identified ca. 2,300 different plant and fungi taxa, which are gathered and consumed in the mediterranean. among these, >1,000 are only consumed in one single zone, therefore ...200616917173
geological history and within-island diversity: a debris avalanche and the tenerife lizard gallotia galloti.several processes have been described that could explain geographical variation and speciation within small islands, including fragmentation of populations through volcanic eruptions. massive landslides, or debris avalanches, could cause similar effects. here we analyse the potential impact of the 0.8 million-year-ago (ma) güimar valley debris avalanche on the phylogeography of the lizard gallotia galloti on the canary island of tenerife. distributions of mitochondrial dna lineages (based on cyt ...200617032262
the canary in the mind: on the fate of dreams in psychoanalysis and in contemporary culture.dreams have been central in the birth and evolution of psychoanalysis. this paper explores the remarkable story of the relationship between dreams and psychoanalysis as a modern version of the long history of dreams in most healing traditions. but psychoanalysis seems to have turned away from dreams as central inspiration in a way parallel to the general culture's turn away from dreams and the reality of inner life. yet modern postindustrial culture is transfixed by a version of "dream life" in ...200616909221
[etiology of acute viral respiratory tract infections in children from gran canaria, the canary islands (spain)].acute respiratory tract infections (arti) of viral origin are a frequent cause of pediatric consultations and hospital admissions. the aim of this study was to investigate the etiology of these infections in gran canaria, the canary islands, (spain).200617125674
diabetes mellitus and impaired glucose regulation in the canary islands (spain): prevalence and associated factors in the adult population of telde, gran canaria.to estimate the prevalence and the determinants of diabetes mellitus and impaired glucose regulation (igr) in an adult canarian population.200616433712
gerronema wildpretii sp. nov. (agaricales, basidiomycetes) a new species from the canary islands.gerronema wildpretii, collected in climactic sites of the monteverde forest of the canary islands is described and illustrated. its macro- and microscopic features delimit this taxon as a new species.200617040074
effect of climatic change in children with atopic eczema.climate and sunlight (ultraviolet radiation) influence activity of atopic eczema.200617073869
determination of changes in blood pressure during administration of sildenafil (viagra) in patients with spinal cord injury and erectile dysfunction.prospective, open-label, comparative study, to assess the effects of sildenafil on blood pressure in a population of patients with spinal cord injury (sci).200616249788
successful management of mite-allergic asthma with modified extracts of dermatophagoides pteronyssinus and dermatophagoides farinae in a double-blind, placebo-controlled study.mite sensitivity is highly prevalent in tropical and subtropical regions, such as the canary islands.200617088125
[analysis of the formulation of policies on aging in plans for social and health care and care of the elderly in autonomous communities in spain].to describe and compare the formulation of policies on aging in the autonomous communities of spain available in 2002.200616942717
scientific meeting of the american institute for psychoanalysis. the canary in the mind: on the fate of dreams in psychoanalysis and in contemporary culture. 200616964537
impact on health-related quality of life and perceived burden of informal caregivers of individuals with alzheimer's disease.this study assessed the impact on health-related quality of life (hrql) and the perceived burden of informal caregivers of individuals with alzheimer's disease (ad) on the canary islands (spain). we utilized a multicenter, cross-sectional design, based on questionnaire responses of 237 informal caregivers of ad patients. patients were classified according to the degree of severity utilizing the clinical dementia rating scale. sociodemographic, hrql (eq-5d) and functional dependency data were gat ...200616974108
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