Publications

TitleAbstractYear(sorted ascending)
Filter
PMID
Filter
changes in airway inflammation and hyperresponsiveness after inhaled corticosteroid cessation in allergic asthma.most patients with asthma are currently controlled by pharmacotherapeutic means such as inhaled corticosteroid (ics). however, whether ics actually induces remission of asthma remains unknown. the present study evaluates changes in airway inflammation and hyperresponsiveness in adult patients with asthma after stopping ics.201020523062
comparison of the skin-prick test and phadia immunocap as tools to diagnose house-dust mite allergy.when the skin-prick test (spt) and in vitro test such as immunocap assay are performed simultaneously, results do not always coincide in some patients. our objectives, therefore, were (1) to assess differences in allergic test results according to age group and (2) to establish appropriate guidelines for diagnosing mite allergy according to age.201020537291
[analyzing of the inhaled allergens profiles of allergic rhinitis patients in district of jingmen].to investigate the profiles of inhaled allergens in allergic rhinitis patients in district of jingmen, and to provide basic epidemiologic information for the prevention and treatment of allergic rhinitis in this area.201020556967
geographical variations in the prevalence of atopic sensitization in six study sites across canada.geographical variations in atopic sensitization in canada have not been described previously. this study used the standardized protocol of the european community respiratory health survey-1 (ecrhs-1) to investigate the distribution and predictors of atopic sensitization in six sites across canada and to compare the results with some ecrhs-1 centers.201020557300
efficacy of high-dose sublingual immunotherapy in children allergic to house dust mites in real-life clinical practice.the aim of the study was to evaluate the efficacy and safety of high-dose mite sublingual immunotherapy (slit) administered in children with allergic rhinitis in real-life clinical practice. moreover, we analysed the clinical course of asthma severity.201020570032
gpr17 regulates immune pulmonary inflammation induced by house dust mites.antagonists of the type 1 cysteinyl leukotriene receptor (cyslt(1)r) are efficacious for bronchoconstriction in humans with bronchial asthma; however, the clinical response to these drugs is heterogeneous. in particular, how cyslt(1)r expression and function are constitutively regulated in vivo is not known. in this study, we show that a seven-transmembrane receptor, gpr17, negatively regulates the cyslt(1)r-mediated inflammatory cell accumulation in the bronchoalveolar lavage fluid and lung, th ...201020574000
aspergillus fumigatus synergistically enhances mite-induced allergic airway inflammation.infection is an important trigger of exacerbation of bronchial asthma. the fact that aspergillus fumigatus (af) causes a distinct clinical syndrome, allergic bronchopulmonary aspergillosis (abpa), suggests unique immunological properties in allergic asthma. the present study aimed to determine how af enhances preexisting allergic airway inflammation and colonizes the airway in asthma.201020581767
enzymatic activities of allergen extracts from three species of dust mites and cockroaches commonly found in korean home.allergen extracts from dust mites and cockroaches commonly found in korean homes were used to evaluate their enzymatic activity as they are believed to influence allergenicity. allergen extracts were prepared from 3 dust mite species (dermatophagoides farinae, d. pteronyssinus, and tyrophagus putrescentiae) and 3 cockroach species (blattella germanica, periplaneta americana, and p. fuliginosa) maintained in the korea national arthropods of medical importance resource bank. proteins were extracte ...201020585532
clinical and immunologic effects of sublingual immunotherapy on patients with allergic rhinitis to house-dust mites: 1-year follow-up results.there have been no data on sublingual immunotherapy (slit) in asian patients with allergic rhinitis (ar) sensitized to house-dust mites (hdms). this study aimed to investigate the efficacy and immunologic change after 12 months of slit in korean patients.201020819465
orally supplemented lactobacillus acidophilus strain l-92 inhibits passive and active cutaneous anaphylaxis as well as 2,4-dinitroflurobenzene and mite fecal antigen induced atopic dermatitis-like skin lesions in mice.oral supplementation of lactic acid bacteria is a potential approach to the prevention and manipulation of allergic diseases such as atopic dermatitis. our previous report showed that heat-killed lactobacillus acidophilus strain l-92 (l-92) possessed anti-allergic properties, although its physiological function in atopic dermatitis has largely remained undefined. to evaluate the anti-allergic efficacy of l-92, we used four experimental animal models with the major features of atopic dermatitis a ...201020840151
sensitization to dermatophagoides, blomia tropicalis, and other mites in atopic patients.house dust mites (hdms) are important causes of persistent allergic diseases, such as asthma and rhinitis. various types of mites are found in the house dust of many countries, including israel.201020846087
characterisation of sarcoptes scabiei antigens.in pig herds, the status of sarcoptes scabiei infections is routinely monitored by serodiagnosis. crude antigen for elisa is usually prepared from s. scabiei var. canis or other variations and may lead to variations in the outcome of different tests, making assay standardisation difficult. this study was performed to investigate the antigen profiles of s. scabiei, including differences between hydrophilic and more hydrophobic protein fractions, by western blotting with sera from pigs with define ...201020865427
comparative analyses of proteolytic activities in seven species of synanthropic acaridid mites.microplate assays with 96 wells were optimized to screen proteolytic activities in mite homogenates. whole-mite extracts of acarus siro, aleuroglyphus ovatus, tyrophagus putrescentiae, tyroborus lini, carpoglyphus lactis, lepidoglyphus destructor, and dermatophagoides farinae exhibited non-specific proteolytic activity in buffers from ph 2 to 12, and three peaks of highest activity at ph 3, 5-6, and 10 were distinguished. the reducing agent tris(2-carboxyethyl)phosphine hydrochloride decreased g ...201020936642
identification of neryl formate as the airborne aggregation pheromone for the american house dust mite and the european house dust mite (acari: epidermoptidae).the american house dust mite, dermatophagoidesfarinae hughes, and european house dust mite, dermatophagoides pteronyssinus trouessart, are major pests of medical importance throughout the developed world, causing atopic diseases such as asthma, rhinitis, and atopic dermatitis. previous studies in our laboratory have shown that the behavioral responses of house dust mites toward volatiles from food sources could be assessed using a y-tube olfactometer assay. the current study used this y-tube ass ...201020939374
cloning, expression, and characterization of der f 7, an allergen of dermatophagoides farinae from china.a full-length cdna encoding house dust mite allergen der f 7 from dermatophagoides farina (acari: pyroglyphidae) from china was cloned, sequenced, and successfully expressed. a reference sequence (genbank accession ay283292) was used to design polymerase chain reaction primers. analysis revealed eight mismatched nucleotides in five der f 7 cdna clones, and the projected amino acid sequence contained six incompatible residues. these results suggest that the sequence of der f 7 may be polymorphic. ...201020939383
dietary supplementation with fructooligosaccharides attenuates airway inflammation related to house dust mite allergen in mice.fructooligosaccharides (fos) are prebiotic supplements that can enhance immunological responses in the host to activate mucosal immunity, probably through regulation of gastrointestinal microflora. an area that has not been investigated, however, is the therapeutic potential of prebiotics on allergic airway diseases. the purpose of this study is to evaluate the effects of dietary supplementation with fos on a murine model of allergic airway inflammation induced by the house dust mite allergen de ...201020943042
evaluation of serum specific ige for the diagnosis of allergic rhinitis with multi-allergens.objective evaluation of allergic rhinitis (ar) requires in vivo and in vitro tests. in vitro tests are important to assist or ensure the main allergens in multi-allergen-sensitive patients. the aim of this study was to evaluate the utility of serum specific ige (sige) in the diagnosis of ar patients with multi-allergens in the chinese population.201021034593
[compliance and safety evaluation of subcutaneous versus sublingual immunotherapy in mite-sensitized patients with allergic rhinitis].to investigate the safety profile of subcutaneous immunotherapy (scit) versus sublingual immunotherapy (slit) in patients with allergic rhinitis (ar) caused by house dust mites. the treatment compliance and related factors were also evaluated.201021055319
identification of astigmatid mites using its2 and coi regions.identification of astigmatid mites based on their morphological characteristics is difficult because of the similarity of their organs, especially in immature mites. the ribosomal second internal transcribed spacer (its2) and the mitochondrial cytochrome oxidase subunit i (coi) regions are highly conserved in the eukaryotes and are usually used as barcodes. the its2 and coi regions of six species of astigmatid mites (aleuroglyphus ovatus, blomia tropicalis, dermatophagoides farinae, dermatophago ...201021072538
topical application of rehmannia glutinosa extract inhibits mite allergen-induced atopic dermatitis in nc/nga mice.rehmannia glutinosa is known in asia as a traditional herbal medicine with anti-inflammatory properties. atopic dermatitis (ad) is an inflammatory skin disease associated with enhanced t-helper 2 (th2) lymphocyte responses to allergens that results in elevated serum ige levels and leukocyte infiltration. although some studies have shown that rehmannia glutinosa extract (rge) has anti-inflammatory and anti-allergic activities, these properties have not been demonstrated in ad. this study investig ...201021129471
stratum corneum removal facilitates experimental sensitization to mite allergens in atopic dogs.in humans with atopic dermatitis and in mouse models of ige-mediated allergic diseases, evidence is mounting that the stratum corneum (sc) provides an important barrier against environmental allergens. at this time, it is not known whether the sc has a similar role in dogs, especially in those with atopic dermatitis. the objectives of this pilot study were to determine whether sc removal led to earlier and stronger sensitization of atopic dogs to dermatophagoides farinae (df) house dust mites. f ...201021143539
cloning, expression, and analysis of the group 2 allergen from dermatophagoides farinae from china.to obtain the recombinant group 2 allergen product of dermatophagoides farinae (der f 2), the der f 2 gene was synthesized by rt-pcr. the full-length cdna comprised 441 nucleotides and was 99.3% identical to the reference sequence (genbank ab195580). the cdna was bound to vector pet28a to construct plasmid pet28a(+)-der f 2, which was transformed into e. coli bl21 and induced by iptg. sds-page showed a specific band of about 14kda in the hole cell lysate. as estimated by chromatography, about 3. ...201021152769
[usefulness of total ige in predicting positive allergen specific ige tests in korean subjects].total ige levels in allergic patients tend to be higher than those in healthy individuals. we evaluated the usefulness of total ige levels in predicting positive results of allergen specific iges in multiple allergen simultaneous tests.201021157154
[acarological faunistic and statistical analysis of dust from various compartments of human dwellings in north-western poland].the aim of this paper was to compare the species composition of mites in dust samples collected at different compartments of human dwellings of north-western (nw) poland. in 30 urban apartments (szczecin, police, pyrzyce and łobez) and rural quarters (przelewice, brzesko szczecińskie, bylice, krasne, kakolewice, wierzchowo, przeradz, kłodzino) from nw poland, 150 samples of dust were collected at 5 locations: bed area, bedroom carpet, sitting area in the living room, hall carpet, and kitchen flo ...201020450008
presence and density of domestic mites in the microenvironment of mite-sensitive dogs with atopic dermatitis.this study was designed to investigate the presence and density of domestic mites (dms) in households with atopic dogs sensitive to these mites (group a; n=20), in households with clinically healthy, nonatopic dogs (group b; n=20) and in households without pets (group c; n=25). dust samples were vacuum-collected from the owner mattress (all groups) and dog sleeping area (groups a and b) or living room couch (group c) on four consecutive occasions, reflecting the four seasons of the year. dms wer ...201020456719
prostaglandin e(2) exerts homeostatic regulation of pulmonary vascular remodeling in allergic airway inflammation.nonselective inhibition of pg synthesis augments inflammation in mouse models of airway disease, but the roles of individual pgs are not completely clarified. to investigate the role of pge(2) in a mouse model of airway inflammation induced by a natural allergen, we used mice lacking the critical terminal synthetic enzyme, microsomal pge(2) synthase (mpges)-1. mice lacking mpges-1 (ptges(-/-) mice) and wild-type c57bl/6 controls were challenged intranasally with low doses of an extract derived f ...201020028661
sublingual immunotherapy efficacy of dermatophagoides farinae vaccine in a murine asthma model.allergen-specific sublingual immunotherapy is a potential treatment for allergic diseases. its effective dose and underlying mechanism are still to be explored. here, we investigated the efficacy and mechanism of sublingually administered dermatophagoides farinae (der f) vaccine in a murine asthma model.201019940504
[skin prick tests with standardized extracts of mites of different precedence in patients with asthma and allergic rhinitis].background: house dust mites are important airborne allergens worldwide, particularly in the tropical environment. objective: to assess sensitization of allergic patients to different mite species in the cuban city of camaguey; as well as to compare standardized allergen extracts from different sources by skin prick tests. patients and method: skin prick tests were performed in 60 patients with asthma and/or allergic rhinitis and in 60 non-allergic volunteers, ran ...201021944487
effects of polyunsaturated fatty acids on isolated canine peripheral blood mononuclear cells and cytokine expression (il-4, ifn-gamma, tgf-beta) in healthy and atopic dogs.polyunsaturated fatty acids (pufa) have been used to treat dogs with atopic dermatitis but the mechanism of action has not been well understood. the aim of this study was to evaluate the in vitro influence of pufa on canine peripheral blood mononuclear cells (pbmc). pbmc isolated from eleven dogs with atopic dermatitis and eleven healthy control dogs were stimulated with concanavalin a and dermatophagoides farinae extract in the presence of linoleic acid (la), gamma-linolenic acid (gla), alpha-l ...201020187917
airway hyperresponsiveness is associated with total serum immunoglobulin e and sensitization to aeroallergens in korean adolescents.allergen sensitization and airway hyperresponsiveness (ahr) are the most important characteristics of bronchial asthma and their correlation has been speculated.201020812252
hypersensitivity to house dust mite and cockroach is the most common allergy in north of iran.asthma and allergic rhinitis are among the most common diseases in the world.201021189446
role of pollen allergy in taiwanese patients with allergic rhinitis.pollen allergy is believed to be less common in east asia, latin america, and tropical areas. the purpose of this study was to understand the role of pollen allergy in taiwan.201021195885
clinical and histopathological evaluation of dermatophagoides farinae-induced dermatitis in nc/nga mice orally administered bacillus subtilis.probiotic strains have been reported to have the ability to control allergic and inflammatory diseases. in this study, we studied the inhibitory effect of bacillus subtilis (natto) (bs) on atopic dermatitis. the effects of continuous oral administration of bs for 4 weeks on the development of atopic dermatitis induced by dermatophagoides farinae body antigen (df) in nc/nga (nc) mice were evaluated using 4 groups of mice: group (gp) df, df(+) with no administration of bacteria (n=3); gp df/bs, df ...201021206175
effects of microwave radiation on house dust mites, dermatophagoides pteronyssinus and dermatophagoides farinae (astigmata: pyroglyphidae).dermatophagoides pteronyssinus and dermatophagoides farinae mites are commonly found in house dust, and are important sources of allergens affecting humans. various approaches to killing the mites have been examined. this study investigated the mortalities of adult mites exposed to 2,450 mhz microwave radiation produced by 3 ovens at various exposure times and power settings. the ovens all had 3 power settings. the average maximum water temperatures generated at high, medium and low power settin ...201021329308
factors for high-risk asthma in taiwanese children.asthma is one of the major causes of death in otherwise healthy young individuals. however, many of these deaths may have been prevented by more aggressive treatment. to determine factors correlated with a high risk of death in taiwanese children with atopic asthma.201021337908
temperature preference and respiration of acaridid mites.the thermal preferences in a grain mass and respiration at various temperatures in mites (acari: acarididae) of medical and economical importance [acarus siro (l. 1758), dermatophagoides farinae hughes 1961, lepidoglyphus destructor (schrank 1871), and tyrophagus putrescentiae (schrank 1781)] were studied under laboratory conditions. based on the distribution of mites in wheat, triticum aestivum l., grain along a thermal gradient from 10 to 40 degrees c, l. destructor, d. farinae, and a. siro we ...201021309251
comparison of optical rhinometry to acoustic rhinometry using nasal provocation testing with dermatophagoides farinae.to evaluate optical rhinometry (orm) as an objective evaluation of nasal patency using nasal provocation testing with dermatophagoides farinae (df) as compared with acoustic rhinometry.201020647137
analysis of expression and amino acid sequence of the allergen mag 3 in two species of house dust mites-dermatophagoides farinae and d. pteronyssinus (acari: astigmata: pyroglyphidae).in the 90's of the xx century, 2 new and important allergens of house dust mites mites were cloned and sequenced: mag 1 and mag 3. however, the second allergen has been identified to date only in extracts of dermatophagoides farinae [df ]. in this work, we aimed to detect expression of this important allergen and for the first time analyze to the amino acid sequence in other species of house dust mite - dermatophagoides pteronyssinus [dp ]. we were able to confirm the expression of allergen mag ...201020684479
impact of ige-mediated hypersensitivity on nasal mucociliary clearance.to evaluate the effect of ige-mediated hypersensitivity on mucociliary clearance time (mcct) and clinical severity, as indicated by total nasal symptoms score (tnss) and peak expiratory flow index (pefi).201020713757
recurrent hopkin's syndrome: a case report and review of the literature.flaccid paralysis affecting one or more limbs after an asthma attack is a poliomyelitis-like illness known as hopkins' syndrome (hs). although a viral infection or multifactorial immune suppression during an acute attack of bronchial asthma has been proposed to be the mechanism involved in this syndrome, the precise etiopathogenetic mechanism remains unknown. we report a 13-year-old girl who had recurrent acute episodes of myelitis after asthma attacks. she had four episodes of acute flaccid par ...201020692673
group v secretory phospholipase a2 reveals its role in house dust mite-induced allergic pulmonary inflammation by regulation of dendritic cell function.we have previously shown that group v secretory phospholipase a(2) (spla(2)) regulates phagocytosis of zymosan and candida albicans by a mechanism that depends on fusion of phagosomes with late endosomes in macrophages. in this study, we report that group v spla(2) (pla2g5)-null mice exposed to an extract of house dust mite dermatophagoides farinae had markedly reduced pulmonary inflammation and goblet cell metaplasia compared with wild-type (wt) mice. pla2g5-null mice had also impaired th2-type ...201020817863
incrimination of blomia tropicalis as a potent allergen in house dust and its role in allergic asthma in kolkata metropolis, india.the increasing trend in allergic diseases has become obvious in the present day, especially in developing countries like india, because of many factors such as change in ambient air quality, increased air pollution, metamorphic change in living habits and lifestyle, and climate.1 mites present in house dust represent a major source of allergens, resulting in different allergic manifestations all over the world, and hypersensitivity to these dust mites may play a pivotal role in pathogenesis of s ...201023268430
association between serum ige levels and the ctla4 +49a/g and fcer1b -654c/t polymorphisms in korean children with asthma.t cells play a central role in cell-mediated immunity, atopic disease, and asthma. the balance of cd28/cytotoxic t-lymphocyte antigen 4 (ctla4)-derived signal transduction plays an important role in the activation of t cells and an increased immunoglobulin e (ige) response. the aim of the current study was to investigate the association between polymorphisms in the genes encoding both ctla4 and the high-affinity ige receptor 1b (fcer1b) and serum ige levels in korean children with asthma.201020358027
[clinical efficacy and safety of sublingual immunotherapy using standardized dermatophagoides farinae extract for children with combined allergic rhinitis and asthma syndrome].to study the clinical efficacy of sublingual immunotherapy using standardized dermatophagoides farinae extract for children with combined allergic rhinitis and asthma syndrome.201121781561
Seasonal Dynamics of House Dust Mites in Dust Samples Collected From Sleeping Places in North-Western Poland.The most common families of mites found in house dust are Pyroglyphidae, Glycyphagidae and Acaridae; all are a source of many antigens responsible for allergic diseases. The aim of this study was to examine the seasonal dynamics of allergenic mite populations in dust samples collected from sleeping places in apartments in north-western Poland. The mites were isolated from the dust using a saturated saline floating method. In 132 dust samples we determined: Dermatophagoides farinae, Dermatophagoi ...201121824362
sensitization to aeroallergens in korean children: a population-based study in 2010.we performed this study to assess the prevalence of sensitization to aeroallergens and to analyze the difference between prevalence rates according to children's ages and residential areas. in this nationwide cross-sectional study, first grade students of 45 elementary schools and 40 middle schools were randomly selected, and skin prick tests were performed for 18 inhalant allergens between october and november 2010. of 7,829 analyzed subjects, 3,753 (47.9%) were sensitized to at least one aeroa ...201121935271
Correlation of specific IgE to shrimp with cockroach and dust mite exposure and sensitization in an inner-city population.Studies have demonstrated that IgE-binding cross-reactive epitopes between shrimp, cockroach, and house dust mite tropomyosins can account for the presence of detectable IgE to shrimp in patients with cockroach and dust mite allergies.201121872304
effect of sublingual immunotherapy on level of cytokines in pbmcs of patients with allergic asthma.this study examined the possible mechanism of sublingual immunotherapy (slit) in the treatment of allergic asthma. forty asthma patients allergic to dust mite were enrolled. they received slit with dermatophagoides farinae (der. f) drops for one year. thirty healthy subjects served as controls. the levels of il-4 and ifn-╬│ of peripheral blood mononuclear cells (pbmcs) were determined in allergic asthma patients before and after the slit as well as the healthy subjects. the results showed that t ...201121671181
allergen micro-array detection of specific ige-reactivity in chinese allergy patients.allergen micro-arrays are powerful tools for screening of serum ige-reactivity. in this study allergen micro-arrays were used to identify dominating ige-binding allergens and cross-reactivity patterns among selected chinese allergy patients.201122340412
contact and fumigant toxicity of armoracia rusticana essential oil, allyl isothiocyanate and related compounds to dermatophagoides farinae.background: the toxicity to adult dermatophagoides farinae of allyl isothiocyanate identified in horseradish, armoracia rusticana, oil and another 27 organic isothiocyanates was evaluated using contact + fumigant and vapour-phase mortality bioassays. results were compared with those of two conventional acaricides, benzyl benzoate and dibutyl phthalate. results: horseradish oil (24 h lc(50) , 1.54 µg cm(-2) ) and allyl isothiocyanate (2.52 µg cm(-2) ) were highly toxic. benzyl isothiocyanate (lc( ...201122102526
[Therapeautic effect on murine asthma with sublingual use of Dermatophagoides farinae/chitosan nanoparticle vaccine].To prepare Dermatophagoides farinae (Der f)/chitosan nanoparticle vaccine (DCN), and to investigate the effect of sublingual administration with DCN in asthma mice model.201121823315
[a cohort investigation of the allergen and pathogenesis in the patients with allergic rhinitis from three hospital of guangdong province].to study the pathogenesis of the patients with allergic rhinitis diagnosed by skin prick test (spt), especially about the epidemiologic data of the involved allergens.201122384564
dectin-2 mediates th2 immunity through the generation of cysteinyl leukotrienes.the innate signaling pathways for th2 immunity activated by inhaled antigens are not well defined. we previously identified dectin-2 as a receptor for glycans in allergen extracts from the house dust mite dermatophagoides farinae (df) that mediates cysteinyl leukotriene (cys-lt) generation from pulmonary cd11c+ cells and from gm-csf-cultured bone marrow cells (bmcs(gm-csf)). using lentiviral knockdown of dectin-2 in bmcs(gm-csf) and adoptive transfer of df-pulsed bmcs(gm-csf) to sensitize naive ...201121357742
efficacy of sublingual immunotherapy with house dust mite extract in polyallergen sensitized patients with allergic rhinitis.it is suggested that polysensitized patients might not benefit from specific allergic rhinitis immunotherapy as much as monosensitized patients, although further research on this subject is needed.201121704889
acaricidal activities of apiol and its derivatives from petroselinum sativum seeds against dermatophagoides pteronyssinus, dermatophagoides farinae, and tyrophagus putrescentiae.the acaricidal effects of an active constituent derived from petroselinum sativum seeds and its derivatives were determined using impregnated fabric disk bioassay against dermatophagoides farinae , dermatophagoides pteronyssinus , and tyrophagus putrescentiae and compared with that of synthetic acaricide. the acaricidal constituent of p. sativum was isolated by various chromatographic techniques and identified as apiol. on the basis of ld(50) values against d. farinae and d. pteronyssinus, apiol ...201121688847
fluorescence immunoassay using an optical fiber for determination of dermatophagoides farinae (der f1).immunoassay methods are generally used for measuring of allergenic substances. however, they need special facilities, skilled handling, and time-consuming procedure. in this work, a fiber-optic immunoassay system which could measure allergen by fluorescent intensities of immune complexes formed by allergens and fluorescently labeled antibodies was established. immune complexes absorbed on the optical fiber probe surface, and excitation light was injected into the probe, then evanescent field is ...201121271355
observational study of the safety of an ultra-rush sublingual immunotherapy regimen to treat rhinitis due to house dust mites.ultra-rush regimens for administering sublingual immunotherapy to patients with allergies are becoming more widespread. we aimed to assess treatment safety for patients with allergic rhinitis with or without asthma caused by dermatophagoides house dust mites.201120664280
house dust mite sublingual immunotherapy: results of a us trial.few trials of sublingual immunotherapy (slit) in the united states have been reported.201121333346
allergy-related outcomes in relation to serum ige: results from the national health and nutrition examination survey 2005-2006.the national health and nutrition examination survey (nhanes) 2005-2006 was the first population-based study to investigate levels of serum total and allergen-specific ige in the general us population.201121320720
glucosamine improved atopic dermatitis-like skin lesions in nc/nga mice by inhibition of th2 cell development.dysregulated th subset responses, characterized by th2-dominant allergic inflammation, are thought to be central to the pathogenesis of atopic dermatitis (ad). glucosamine has been shown to have immunosuppressive properties, but its effect on ad has not been examined. in this study, the immunoregulatory effects of glucosamine, using dermatophagoides farinae (df)-induced ad-like skin lesions in nc/nga mice, were investigated. the clinical scores were reduced significantly by the treatment with gl ...201121284687
high allergen-specific serum immunoglobulin e levels in nonatopic west highland white terriers.human and canine atopic dermatitis (ad) share an association with ige specific to environmental allergens, but few studies have evaluated serum allergen-specific ige in nonatopic dogs. this study compared serum allergen-specific ige levels in 30 atopic and 18 nonatopic west highland white terriers. atopic dermatitis was confirmed using standard criteria. nonatopic dogs were over 5 years of age and had no clinical signs or history of ad. serum allergen-specific ige levels were measured with aller ...201121265887
suppression of dust mite extract and 2,4-dinitrochlorobenzene-induced atopic dermatitis by the water extract of lindera obtusiloba.ethnopharmacological relevance: the lindera obtusiloba has been used in traditional medicine for the treatment of inflammation and dermatitis. in this study, we investigated the effect of topical application of lindera obtusiloba water extract (lowe) on the house dust mite extract (dermatophagoides farinae extract, dfe) and 2,4-dinitrochlorobenzene (dncb)-induced atopic dermatitis (ad). materials and methods: we established ad model in balb/c mice by repeated local exposure of dfe/dncb to the ea ...201121762765
allergic conjunctivitis and conjunctival provocation tests in atopic dogs.canine atopic dermatitis (cad) is a very common disease, but little is known about eye involvement. the conjunctival provocation test (cpt) is used in human to study the ocular response to allergenic stimuli and to evaluate anti-allergic therapy. to our knowledge it has not been used in dogs.201121733066
effect of inhaled dust mite allergen on regional particle deposition and mucociliary clearance in allergic asthmatics.background acute exacerbations in allergic asthmatics may lead to impaired ability to clear mucus from the airways, a key factor in asthma morbidity. objective the purpose of this study was to determine the effect of inhaled house dust mite challenge on the regional deposition of inhaled particles and mucociliary clearance (mcc) in allergic asthmatics. methods we used gamma scintigraphy (inhalation of (99m) tc -sulphur colloid particles) to measure the regional particle deposition and mcc in all ...201121729182
The purinergic g protein-coupled receptor 6 inhibits effector T cell activation in allergic pulmonary inflammation.We show that the P2Y(6) receptor, a purinergic G protein-coupled receptor with a high affinity for the nucleotide uridine diphosphate, is an important endogenous inhibitor of T cell function in allergic pulmonary inflammation. Mice conditionally deficient in P2Y(6) receptors [p2ry6 (flox/flox);cre/+ mice] exhibited severe airway and tissue pathology relative to P2Y(6)-sufficient [p2ry6 (flox/flox)] littermates (+/+ mice) when treated intranasally with an extract of the dust mite Dermatophagoides ...201121724990
administration of poly(i:c) improved dermatophagoides farinae-induced atopic dermatitis-like skin lesions in nc/nga mice by the regulation of th1/th2 balance.atopic dermatitis (ad) is characterized by a chronic and replapsing skin disease with th2-dominant allergic inflammation. poly(i:c) has been shown to have immunopotentiator properties, but its effect on ad has not been examined. in this study, the immunomodulatory effects of poly(i:c), using dermatophagoides farinae (df)-induced ad-like skin lesions in nc/nga mice, were investigated. the clinical scores were reduced significantly by the treatment with poly(i:c) at 25 and 50μg/mouse. histological ...201122119586
clinical comparison of human and canine atopic dermatitis using human diagnostic criteria (japanese dermatological association, 2009): proposal of provisional diagnostic criteria for canine atopic dermatitis.atopic dermatitis (ad) is a common skin disease encountered in both humans and dogs. canine ad can be used in the analysis of naturally occurring ad; however, details of clinical comparison have been lacking. the purpose of this study is to compare those clinical features using the human diagnostic criteria (japanese dermatological association, 2009). fifty-one dogs with canine ad were evaluated by the human criteria. prior to this study, canine ad was basically diagnosed by the fulfillment of t ...201121434981
evaluation of patch test with airbone allergic agents in patients with atopic dermatitis.atopic dermatitis is an inflammatory skin disease that can be triggered by many factors. several reports confirm the role of airborne allergic agents as aggravating or triggering factors. the patch test with airborne allergic agents or the atopy patch test was suggested to evaluate the role of these allergens in atopic dermatitis.201121437520
the effect of peptidoglycan stimulation on basophil-mediated atopic responses during pregnancy and in newborns.toll-like receptor (tlr) molecules play critical roles in directing the course of atopic diseases by recognizing specific microbial products that activate immune effector cell function.201121438704
new sensitization to house dust mites in cefteram-induced occupational asthma: a case report.occupational asthma (oa) can improve after cessation of exposure; however, some patients suffer from persistence or aggravation of their asthmatic symptoms. here we report a case of a new sensitization to house dust mites during the follow-up period in a 37-year-old female patient with oa induced by cefteram pivoxil powder (cefteram powder). she was previously diagnosed with oa caused by inhalation of cefteram powder. consequently, she left her job and had been well for 9 subsequent years. she b ...201121461254
contact and fumigant toxicity of cinnamaldehyde and cinnamic acid and related compounds to dermatophagoides farinae and dermatophagoides pteronyssinus (acari: pyroglyphidae).toxicities of (e)-cinnamaldehyde and (e)-cinnamic acid and their 41 structurally related compounds to adult dermatophagoides farinae hughes and dermatophagoides pteronyssinus trouessart (acari: pyroglyphidae) were examined using fabric-circle contact plus fumigant and vapor-phase mortality bioassays. results were compared with those of two acaricides, benzylbenzoate and dibutyl phthalate. in contact plus fumigant mortality bioassays, the most toxic compounds were (e)-cinnamaldehyde, methyl (e)-c ...201121485375
da-9601 suppresses 2, 4-dinitrochlorobenzene and dust mite extract-induced atopic dermatitis-like skin lesions.da-9601 (stillen™) is a novel anti-peptic formulation prepared from the ethanol extracts of artemisia asiatica possessing anti-oxidative, anti-allergic and anti-inflammatory activities. however, their effect on atopic dermatitis (ad) has not been studied yet. in this study, we report that topical application of da-9601 suppressed house dust mite extract (dermatophagoides farinae extract, dfe) and 2, 4-dinitrochlorobenzene (dncb)-induced ad-like skin lesions in balb/c mice model. we established a ...201121511060
lack of preventing effect of systemically and topically administered histamine h(1) or h(4) receptor antagonists in a dog model of acute atopic dermatitis.as there is evidence for an anti-inflammatory efficacy of histamine h(4) receptor (h4r) selective antagonists, we aimed at testing the efficacy of the h4r antagonists jnj7777120 and jnj28307474 in comparison with histamine h(1) receptor (h1r) antagonists hydroxyzine and cetirizine for skin lesion prevention in a canine model of acute atopic dermatitis. six atopic maltese-beagle dogs experimentally sensitized to dermatophagoides farinae (df) house dust mites were selected for this study. twenty-f ...201121521369
mite exposure in a spanish mediterranean region.background: knowledge of the domestic mite fauna and allergen levels is important for a correct diagnosis and treatment of mite allergy. our objectives were to describe the domestic mite fauna in the region of murcia, spain, to quantify mite allergens in dust samples obtained from mattresses of this area and to assess the influence of geographical, climatic and dwelling factors. methods: dust samples were collected in a transversal descriptive study from mattresses of 51 patients who went to the ...201121550163
in-vivo allergenic potency of siriraj mite allergen vaccine (smav) comparing with standardized vaccine in mite-sensitive patients.the prevalence of allergic diseases, particularly asthma and allergic rhinitis, has increased tremendously in thailand and worldwide. house dust mite (hdm) is the major ige sensitizer among allergic children and adults. we have developed local standardized mite allergen extracts, siriraj mite allergen vaccine (smav) from dermatophagoides pteronyssinus (dp) and dermatophagoides farinae (df) from our source materials which were highly purified (99%).201121560488
quantification of der f 1 in houses of patients allergic to house dust mite, dermatophagoides farinae, using a locally produced detection reagents.house dust mite (hdm) allergen quantification in house dust samples before and after the allergen elimination is one means of convincing the target population about the health benefits of allergen removal from their environment.201121560492
immunomodulation of skin cytokine secretion by house dust mite extracts.background: skin contact with house dust mites may contribute to atopic dermatitis and other skin diseases. we sought to determine if molecules from house dust mites could influence the release of proinflammatory cytokines and chemokines from epidermal keratinocytes and dermal fibroblasts grown in a human skin equivalent (hse) model. methods: hses consisting of an epidermis of keratinocytes with stratum corneum over a dermis of fibroblasts in a collagen matrix were challenged with dermatophagoid ...201121576987
allergenic dermatophagoides mites causing asthma among schoolchildren at ain-shams district, cairo, egypt.the present study was performed on house dust samples collected from ten homes of schoolchildren suffering from asthma at ain-shams district, over a period of two years (2008 & 2009). the data revealed that the total annual density of the two allergenic mites (dermatophagoides pteronyssinus (trouessart) & dermatophagoides farinae (hughes) was 202 individual mites with 22 asthmatic children in the first year, and individual mites increased to 268 with 36 asthmatic children in the second year. the ...201121634241
[Aeroallergen spectrum of patients with allergic rhinitis in Enshi area].To investigate aeroallergen spectrum of patients with allergic rhinitis in Enshi area and analyze its related factors.201122032124
prior or coinstantaneous oral exposure to environmental immunosuppressive agents aggravates mite allergen-induced atopic dermatitis-like immunoreaction in nc/nga mice.immunosuppressive environmental chemicals may increase the potency of allergens and thereby play a role in the development of allergic diseases such as allergic rhinitis, asthma and atopic dermatitis (ad).201121864637
skin prick testing to aeroallergen extracts: what is the optimal panel in children and adolescents in turkey?background/objective: the skin prick test (spt) is the standard tool for the diagnosis of ige-mediated allergic diseases. the number and spectrum of allergen extracts to be applied for the diagnosis of sensitized patients in order to achieve the most cost-effective and informative results are critical from both economic and scientific viewpoints. methods: in order to determine the minimum test battery panel necessary to cover at least 95% of the cases of spt sensitization in children and adolesc ...201122123205
histamine and allergen skin reactivity in the elderly population: results from the korean longitudinal study on health and aging.the allergen skin prick test is the most representative diagnostic test for atopy, but its use has been limited because of the belief that skin reactivity decreases with aging.201121962095
significance of specific igg against sensitizing antigens in extrinsic allergic alveolitis: serological methods in eaa.the aim of our study is to find differences in igg in sera of potentially exposed and nonexposed individuals and to detect differences in concentrations of specific serum igg among subjects with and without eaa. seventy-two patients being followed for suspected interstitial lung disease were included. specific igg in sera were established by immunocap. serum concentrations of aspergillus fumigatus, candida albicans igg and mixture of moulds igg were higher in subjects with exposure to relevant i ...201121924863
Detection and Identification of Species-Specific Bacteria Associated with Synanthropic Mites.Internal bacterial communities of synanthropic mites Acarus siro, Dermatophagoides farinae, Lepidoglyphus destructor, and Tyrophagus putrescentiae (Acari: Astigmata) were analyzed by culturing and culture-independent approaches from specimens obtained from laboratory colonies. Homogenates of surface-sterilized mites were used for cultivation on non-selective agar and DNA extraction. Isolated bacteria were identified by sequencing of the 16S rRNA gene. PCR amplified 16S rRNA genes were analyzed b ...201122057398
Obesity-associated asthma in children- a distinct entity.Abstract BACKGROUND:Obesity-associated asthma has been proposed to be a distinct entity, differing in immune pathogenesis from atopic asthma. Both obesity-mediated inflammation and increase in adiposity are potential mechanistic factors that are poorly defined among children. We hypothesize that pediatric obesity-associated asthma will be characterized by T helper 1(Th1), rather than the Th2 polarization associated with atopic asthma. Moreover, we speculate that Th1 biomarkers and anthropometric ...201121980061
a comparison between ige and igg4 as markers of allergy in children: an experimental trial in a model of natural antigen avoidance.igg4 have been hypothesized to act as blocking antibodies capable of preventing ige-mediated effector cell triggering. this study aims to evaluate the changes in igg4 in children during a period of natural antigen avoidance. serum ige and igg4 were evaluated in a group of asthmatic children, aged between 7 and 17 years, admitted to the residential house istituto pio xii (misurina, bl, italy), located at 1,756 m, in a natural model of antigen avoidance. all the patients were skin prick test posit ...201122230410
ige responsiveness to dermatophagoides farinae in west highland white terrier dogs is associated with region on cfa35.immunoglobulin e (ige)-mediated hypersensitivity against environmental allergens, commonly including dermatophagoides farinae, is associated with atopic diseases in both humans and dogs. we have recently identified a family of clinically healthy west highland white terriers (whwts) with high-serum d. farinae-ige levels. in this study, we investigated the genetic mechanism controlling ige responsiveness in dogs by performing a genome-wide association study (gwas) using the affymetrix v2 dog snp a ...201121846750
evaluation of therapeutic sublingual vaccines in a murine model of chronic house dust mite allergic airway inflammation.second generation therapeutic vaccines based upon recombinant allergens or natural extracts, potentially formulated in vector systems or adjuvants, are being developed. to this aim, preclinical studies in relevant animal models are needed to select proper allergens, formulations and administration schemes.201122092967
The Fruits of Cudrania tricuspidata Suppress Development of Atopic Dermatitis in NC/Nga Mice.The fruits of Cudrania tricuspidata are a medicinal herb in Korea, known for its antiatherosclerotic and antiinflammatory effects. Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by the influx of lymphocytes into the dermis. Using an animal model of AD, we assessed whether C. tricuspidata suppresses the development of AD-like skin lesions. Cudrania tricuspidata was administered orally to NC/Nga mice with Dermatophagoides-farinae-induced AD-like lesions for 49?days. Th ...201121972057
exhaled nitric oxide is associated with allergic inflammation in children.exhaled nitric oxide (eno) has been proposed as a noninvasive marker of airway inflammation in asthma. in asthmatic patients, exhaled no levels have been shown to relate with other markers of eosinophilic recruitment, which are detected in blood, sputum, bronchoalveolar lavage fluid and bronchial biopsy samples. the purpose of this study was to assess the possible relationship between eno and allergic inflammation or sensitization in childhood asthma and allergic rhinitis. subjects consisted of ...201122022176
In planta expression of a mature Der p 1 allergen isolated from an Italian strain of Dermatophagoides pteronyssinus.European (Dermatophagoides pteronyssinus) and American (Dermatophagoides farinae) house dust mite species are considered the most common causes of asthma and allergic symptoms worldwide. Der p 1 protein, one of the main allergens of D. pteronyssinus, is found in high concentration in mites faecal pellets, which can became easily airborne and, when inhaled, can cause perennial rhinitis and bronchial asthma. Here we report the isolation of the Der p 1 gene from an Italian strain of D. pteronyssinu ...201121904913
association of house dust mite-specific ige with asthma control, medications and household pets.evidence is conflicting regarding the effectiveness of creating a low-allergen environment or reducing allergen exposure to control asthma exacerbations.201122053311
[efficacy of sublingual immunotherapy with dermatophagoides farinae drops in patients with allergic rhinitis].to evaluate the efficacy of sublingual immunotherapy (slit) with dermatophagoides farinae drops in patients with allergic rhinitis (ar).201122321427
effect of respiratory syncytial virus infection on regulated on activation, normal t-cells expressed and secreted production in a murine model of asthma.synthesis of regulated on activation, normal t-cells expressed and secreted (rantes) in the airway has previously been shown to be elevated after respiratory syncytial virus (rsv) infection. however, since few studies have examined whether rsv-infected asthma patients express a higher level of rantes than do normal individuals, we used a murine model of asthma to address this question.201122253642
immunotherapy is allergen-specific: a double-blind trial of mite or timothy extract in mite and grass dual-allergic patients.one hundred years ago, noon [lancet 1911;1:1572-1573], using conjunctival provocation testing (cpt), was the first to demonstrate the effectiveness of subcutaneous immunotherapy (scit) in grass-allergic subjects with hay fever. in this centenary year, we present data that, by use of cpt and allergen-specific igg, replicate this observation and additionally confirm the allergen specificity of scit by using a double-blind design employing either grass or mite scit in dual grass- and mite-allergic ...201122212720
acaricidal activity of cymbopogon citratus and azadirachta indica against house dust mites.to examine the acaricidal effects of the essential oil of cymbopogon citratus leaf extract (lemongrass) and ethanolic azadirachta indica leaf extract (neem) against house dust mites dermatophagoides farinae (d. farinae) and dermatophagoides pteronyssinus (d. pteronyssinus).201123569794
oral curcumin supplementation in patients with atopic asthma.oral curcumin is recognized to have anti-inflammatory properties and has been used by ancient traditional medicine for centuries to treat a variety of diseases. in vitro studies have confirmed the ability of curcumin to inhibit allergic inflammatory cytokine responses from lymphocytes; however, there are no in vivo studies of curcumin to treat inflammation associated with allergic asthma. this study was designed to determine the effect of oral curcumin supplementation on patients with stable, pe ...201122852117
Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of a major group 7 allergen, Der f 7, from the dust mite Dermatophagoides farinae.Der f 7 is a major group 7 allergen from the dust mite Dermatophagoides farinae that shows 86% sequence identity to the homologous allergen Der p 7 from D. pteronyssinus. Der f 7 was successfully overexpressed in an Escherichia coli expression system and purified to homogeneity using Ni-NTA affinity and size-exclusion column chromatography. SeMet-labelled Der f 7 was crystallized by the hanging-drop vapour-diffusion method using a reservoir solution consisting of 0.1 M bis-tris pH 7.4 and 28% po ...201122139179
[aeroallergen spectrum of patients with child allergic rhinitis in changsha area of china].to investigate aeroallergen spectrum and allergy positive rates of patients with child allergic rhinitis and analyze its related factors.201122070068
acaricidal activities of clove bud oil and red thyme oil using microencapsulation against hdms.the purpose of this study was to produce a safer microcapsule loaded with clove bud oil and red thyme oil to reduce the population of house dust mites (hdms). gelatin-based microcapsules 4-85 µm in size were created, with agitation speed and type of oil playing a critical role in governing their size. microcapsules made up of single spherical units less than 30 µm in diameter remained separate on the fibre, whereas larger microcapsules of over 30 µm ruptured or aggregated. thermogravimetric anal ...201121171819
Refresh
firstprev
...111213141516
nextlast
Displaying items 1001 - 1100 of 1559