Publications

structure and macromolecular assembly of two isoforms of the major sperm protein (msp) from the amoeboid sperm of the nematode, ascaris suum.	ascaris sperm are amoeboid cells that crawl by extending pseudopods. although amoeboid motility is generally mediated through an actin-based cytoskeleton, ascaris sperm lack this system. instead, their major sperm protein (msp) forms an extensive filament system that appears to fulfil this function. because their motility appears to be essentially the same as that of their actin-rich counterparts, ascaris sperm offer a simple alternative system for investigation of the molecular mechanism of amo ...	1992	1527183
resistance to ascaris suum in parasite naïve and naturally exposed growers, finishers and sows.	commercially reared growers, finishers, and sows of danish landrace x yorkshire crossbred were inoculated orally with ascaris suum at 50 eggs kg-1 body weight. white spots on the serosal surface of livers and total larval recoveries from lungs were recorded 7 days later. the response in pigs originating from a specific pathogen free and parasite free herd (parasite naïve) was observed in the three different age groups and compared with age-matched pigs from a herd maintained in a facility contam ...	1992	1532874
response to repeated inoculations with ascaris suum eggs in pigs during the fattening period. i. studies on worm population kinetics.	this experimental study on pigs was designed to simulate natural, long-term exposure to ascaris suum under modern management conditions. parasite kinetics were followed in pigs receiving a. suum eggs as repeated trickle inoculations at two dose levels beginning at a body weight of 25 kg until their slaughter at 90 kg (baconers). in pigs inoculated twice weekly with 500 eggs, there was an initial marked rise in the numbers of hepatic milk spots, but as early as around week 6 after the start of in ...	1992	1534170
association of a heat-stable inhibitor protein with cyclic-3',5'-amp-dependent protein kinase from the nematode ascaris suum: purification and characterization of the inhibitor.	an inhibitor protein of the catalytic subunit of the cyclic 3',5'-amp-dependent protein kinase from the nematode ascaris suum was isolated and characterized. the molecular weight of the inhibitor was estimated as 28,000 by electrophoresis under denaturing conditions and as 30,000 by gel permeation chromatography on superose 12. the trypsin-labile inhibitor was resistant to short incubations (less than or equal to 5 min) at temperatures up to 95 degrees c and at ph 3. it affected the protein kina ...	1992	1497350
the 5-lipoxygenase inhibitor zileuton blocks antigen-induced late airway responses, inflammation and airway hyperresponsiveness in allergic sheep.	leukotrienes are thought to be involved in allergen-induced airway responses. to test this hypothesis we used a newly described 5-lipoxygenase inhibitor, zileuton, and examined its effect on antigen-induced early and late bronchial responses, airway inflammation and airway hyperresponsiveness in allergic sheep. early and late responses were determined by measuring specific lung resistance (srl) before and serially for 8 h after antigen challenge. airway inflammation was assessed by bronchoalveol ...	1992	1330588
voltage-activated currents in somatic muscle of the nematode parasite ascaris suum.	1. voltage-activated currents in cell bodies of the somatic muscle cells of ascaris suum were studied using a two-microelectrode voltage-clamp technique. cells recorded from had resting membrane potentials around -35 mv and had input conductances in the range 1-10 micros. 2. in cells bathed in artificial perienteric fluid, depolarizing steps from a holding potential of -35 mv elicited outward currents at a threshold of -15 mv. these currents had inwardly directed inflections on the rising phase, ...	1992	1336792
effect of purified components of ascaris suum extracts on ige antibody response in guinea pigs.	the effect of components p530 and p29, isolated from ascaris suum adult worm extract (asc), on the heterologous ige antibody response was studied in guinea pigs. groups of 7 guinea pigs were immunized ip with 50 micrograms of ovalbumin (oa) alone or mixed with 200 micrograms of each component precipitated in an alum gel. the primary and secondary ige antibody response was evaluated by passive cutaneous anaphylaxis reaction (pca). immunization of guinea pigs with p29 plus ovalbumin (oa) resulted ...	1992	1342594
immunologic mast cell-mediated responses and histamine release are attenuated by heparin.	heparin has been shown to act as a competitive inhibitor of inositol 1,4,5-triphosphate (insp3) receptors in various cell types. because insp3 is one of the second messengers involved in stimulus-secretion coupling in mast cells, it is possible that heparin may inhibit mast cell-mediated reactions. therefore, in allergic sheep, we tested this hypothesis in two mast cell-mediated reactions induced by immunologic and nonimmunologic stimuli: immediate cutaneous reaction (icr) and acute bronchoconst ...	1992	1383185
inhibitory reflex during bronchoconstrictions induced by histamine and ascaris suum antigen.	we investigated the involvements of sympathetic and nonadrenergic nervous systems in the inhibitory reflex following bronchoconstriction in dogs. inhalations of a 0.00125% solution of histamine and ascaris suum antigen (3 mg protein) to the bronchial side induced reflex tracheal constriction following bronchoconstriction. an intra-arterial infusion of 5 micrograms/min of atropine to the tracheal site changed the reflex tracheal constrictions by histamine and antigen inhalations into tracheal dil ...	1992	1405031
polar zipper sequence in the high-affinity hemoglobin of ascaris suum: amino acid sequence and structural interpretation.	the extracellular hemoglobin of ascaris has an extremely high oxygen affinity (p50 = 0.004 mmhg). it consists of eight identical subunits of molecular weight 40,600. their sequence, determined by protein chemistry, shows two tandemly linked globin-like sequences and an 18-residue c-terminal extension. two n-linked glycosylation sites contain equal ratios of mannose/glucosamine/fucose of 3:2:1. electron micrographs suggest that the eight subunits form a polyhedron of point symmetry d4, or 42. the ...	1992	1584800
pulmonary vascular response to anaphylaxis in isolated canine lungs.	we determined changes in vascular resistance and microvascular permeability during anaphylactic reaction in isolated canine lungs perfused at constant pressure with autologous blood. in lungs with anaphylaxis induced by an intra-arterial injection of ascaris suum antigen (10 mg), pulmonary vascular resistance and capillary pressure assessed as double occlusion pressure increased transiently by 10 times and 6.3 mmhg, respectively. pre- to postcapillary vascular resistance ratio decreased from 0.8 ...	1992	1443218
mechanism of activation of the nad-malic enzyme from ascaris suum by fumarate.	the mechanism of activation of the nad-malic enzyme from ascaris suum by fumarate has been probed using initial velocity studies, deuterium isotope effects, and isotope partitioning of the e:mg:malate complex. fumarate exerts its activating effect by decreasing the off-rate for malate from the e:mg:malate and e:nad:mg:malate complexes. fumarate is a positive heterotropic effector of the nad-malic enzyme at low concentrations (k act approximately 0.05 mm) and an inhibitor competitive against mala ...	1992	1444459
relationship of airway responsiveness to agents causing bronchoconstriction and cough in sensitized guinea-pigs.	the relationship between airway responsiveness to bronchoconstrictor- and cough-inducing stimuli has been examined in ascaris suum-sensitized conscious guinea-pigs. guinea-pigs were sensitized to ascaris suum [4000 pnu and 100 mg al(oh)3 i.p. on days 1 and 7] and then challenged with aerosolized antigen on days 21, 28 and 35. at day 35, antigen-exposure produced an early bronchoconstrictor response (ebr) and in about 50% of the animals also a late bronchoconstrictor response (lbr) commencing 4-8 ...	1992	1446141
biochemical characterisation of the enzyme responsible for "activated l-serine sulphydrase" activity in nematodes.	the biochemical properties of the enzyme responsible for nematode "activated l-serine sulphydrase" activity (l-cysteine + r-sh----cysteine thioether + h2s) have been investigated using primarily the gastro-intestinal nematodes nippostrongylus brasiliensis and haemonchus contortus. the activated l-serine sulphydrase enzyme was found to be cytosolic in origin and exhibited maximal activity at ph 9.0. enzyme activity was widely distributed amongst the major tissues of adult female ascaris suum but ...	1992	1740180
the primary structure of te-6: a novel neuropeptide from the nematode ascaris suum.	extensive immunoreactivity (ir) towards a hexapeptide (sequence kgqele), which flanks the c-terminus of the pancreastatin sequence in rat chromogranin a (cga), is found throughout the nervous system of the nematode parasite ascaris suum. the peptide ir was purified from the gonoduct of the parasite and found to have the sequence tkqele. this peptide, designated te-6, has some c-terminal homology with several regions of the cga molecule. however, te-6 was the only peptide isolated suggesting that ...	1992	1417808
interaction between ascaris suum and pasteurella multocida in the lungs of mice.	in an experiment including 8 groups of 15 mice, the effect of migrating ascaris suum larvae in the lungs on the establishment and pathogenicity of aerosol exposure to pasteurella multocida was investigated. following aerosol exposure to p. multocida, mice with migrating a. suum in their lungs developed more severe pneumonia and septicaemia than did parasite-free mice. the parasite-induced effect on bacterial pathogenicity was more marked for a non-toxin-producing p. multocida as compared with a ...	1992	1438140
[ascaris suum infection as a cause of clinical problems in cattle?].	a sudden decrease in milk yield, increased respiratory rate and occasional coughing were observed in dairy cows on two farms in spring 1991. pigs were also kept on these farms, and pastures grazed by the cattle had been fertilised with pig slurry. laboratory investigations of some of the cattle showed eosinophilia and high elisa titres of antibodies against ascaris suum. on one farm the clinical symptoms disappeared after the animals had been treated with oxfendazole and on the other farm the sy ...	1992	1604447
standardization of dot-elisa for the serological diagnosis of toxocariasis and comparison of the assay with elisa.	the dot-enzyme-linked immunosorbent assay (dot-elisa) was standardized using somatic (s) and excretory-secretory (es) antigens of toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. all samples were used before and after absorption with ascaris suum extract. when the results ...	1992	1307410
mechanistic studies in the transcuticular delivery of antiparasitic drugs. ii: ex vivo/in vitro correlation of solute transport by ascaris suum.	using live, intact ascaris suum and a closed perfusion system, the absorption kinetics and tissue distribution of selected radiolabeled permeants were measured to determine the importance of the transcuticular pathway for drug absorption. the data support the conclusions established by previous in vitro transport studies which utilized excised cuticle-hypocuticle tissue preparations. the external surface of a. suum can be breached by drugs and the rate-determining barrier is the lipoidal hypocut ...	1992	1625697
dna synthesis in the early embryo of the nematode ascaris suum.	we have used microspectrofluorimetry to measure the rate of dna synthesis in the first two embryonic cell cycles of the parasitic nematode ascaris suum. the s-phase of the early ascaris cell cycles occupies at most 1 hr; g2 phase is prominent and occupies approximately 11 hr; no g1 phase could be detected. these results contrast with our previous measurements made with embryos of the free-living nematode caenorhabditis elegans, in which the earliest cell cycles consist of simple alternations bet ...	1992	1628758
ascaris suum: are trypsin inhibitors involved in species specificity of ascarid nematodes?	inhibitors of porcine trypsin were prepared from aqueous extracts of the parasitic nematodes ascaris suum (hogs) and ascaris lumbricoides (human). in this study three experiments were performed. (1) polyclonal antibodies were prepared against one isoform of trypsin inhibitor from each parasitic nematode. each antibody reacted with all isoforms from itself as well as all isoforms from the other parasite. (2) association equilibrium constants were measured by titrating host trypsins (porcine or hu ...	1992	1639157
cross-reactions of sera from toxocara canis-infected mice with toxascaris leonina and ascaris suum antigens.	the elisa method using larval excretory-secretory (e/s) products and homogenized toxocara canis, toxascaris leonina and ascaris suum adult worm extract were used to determine possible cross-reactions in balb/c and c57bl/10 mice, inoculated with embryonated eggs or adult worm extract of t. canis in single and multiple doses. when we used sera of mice infected with embryonated eggs of t. canis against different heterologous antigens, we observed no cross-reactions in balb/c mice against a. suum e/ ...	1992	1639565
extracellular recordings from the motor nervous system of the nematode, ascaris suum.	1. the close association of muscle and neurons in ascaris suum makes it difficult to determine whether spikes recorded from nerve cords originate in muscle or neurons. we have developed criteria that distinguish muscle and neuronal activity. there are two categories of extracellular spikes. 2. the first category consists of spikes with a wide range of amplitudes, marked by large spikes. these spikes, which can be recorded over lateral muscle and over the dorsal and ventral nerve cords, are aboli ...	1992	1328624
on the distribution of a fluorescent ivermectin probe (4" 5,7 dimethyl-bodipy proprionylivermectin) in ascaris membranes.	the distribution of the fluorescent ivermectin derivative (4" 5,7 dimethyl bodipy proprionylivermectin, referred to here as bodipy ivermectin) was studied in muscle vesicle membranes from the parasite ascaris suum. incubation in bodipy ivermectin, at concentrations greater than 1 ng/ml, produced a dose-dependent fluorescence in the vesicles. the level of fluorescence was not depressed (but increased) by prior incubation with 1 microm unlabelled ivermectin. this observation suggested that most of ...	1992	1641253
comparison between the mhc-restricted antibody repertoire to ascaris antigens in adjuvant-assisted immunization or infection.	genetic restrictions to the immune repertoire will be an important consideration in the development of anti-nematode vaccines. it has already been established that the major histocompatibility complex (mhc) limits responsiveness to nematode antigens in infection, but little is known of whether this also applies under other routes of sensitization, such as with adjuvants. the specificity of the antibody response was, therefore, compared in infection and adjuvant-assisted immunization using secret ...	1992	1557231
effect of glucan preparation on immunocompetent cells and phagocytic ability of blood leucocytes in experimental ascariosis of pigs.	in experimental ascariosis (ascaris suum) of pigs a preparation of glucan combined with pig immunoglobulin and zinc was found to significantly elevate the levels of both t and b lymphocytes in the blood, and to significantly stimulate the phagocytic ability of monocytes and neutrophilic granulocytes in experimental pigs. the protective effect of the preparation, assessed by the reduction in the number of migrating ascarid larvae in the lungs of pigs was 65%.	1992	1561757
characterization of cdna clones for the alpha subunit of pyruvate dehydrogenase from ascaris suum.	the pyruvate dehydrogenase complex of the adult parasitic nematode ascaris suum functions in the reducing environment present in their anaerobic mitochondria. these organelles use fumarate and enoyl coas as terminal electron acceptors instead of oxygen. a lambda gt11 cdna library was constructed from rna isolated from adult ascarid muscle. partial clones for the pyruvate dehydrogenase alpha subunit were isolated by screening the lambda gt11 library with a specific antiserum. full-length clones ( ...	1992	1565136
the onset and recovery from airway hyperresponsiveness: relationship with inflammatory cell infiltrates and release of cytotoxic granule proteins.	previous studies from our laboratory have demonstrated a temporal relationship between eosinophil influx into the airways and the onset of airway hyperresponsiveness to inhaled methacholine. the purpose of the present study was to extend this observation by evaluating changes in airway cellular composition and measuring the levels of granulocyte-derived mediators recovered in bal fluid during the onset and recovery from antigen-induced airway hyperresponsiveness. airway cellular composition, air ...	1992	1571822
purification of glutathione reductase from muscle of the adult parasitic nematode ascaris suum.		1992	1574090
mechanisms of microenvironmental ph regulation in the cuticle of ascaris suum.	the excretion kinetics of various organic acids by ascaris suum were quantified to determine if the excretion of these metabolic end-products could generate and maintain a microclimate ph within the aqueous compartment of the cuticle. ligated and nonligated a. suum were incubated in media buffered with 0.25 or 2.5 mm hepes (initial ph 7.5) or 0.5 or 5 mm glycine (initial ph 3.25). the concentration of organic acids and the ph of the media were followed for 24 h. several volatile fatty acids, inc ...	1992	1501633
effects of inhaled heparin on immunologic and nonimmunologic bronchoconstrictor responses in sheep.	the glycosaminoglycan heparin has been shown to block inositol triphosphate (ip3) receptors in various tissues. because ip3 is one of the pathways involved in stimulus-secretion coupling in mast cells, it is possible that heparin may inhibit airway anaphylaxis. thus, the purpose of the present investigation was to study the effect of inhaled heparin on immunologically and nonimmunologically induced bronchoconstriction in allergic sheep. specific lung resistance (srl) was measured in 15 sheep all ...	1992	1546836
ascaris suum--vaccination of mice with liposome encapsulated antigen.	vaccination with liposome encapsulated adult crude antigen with and without coencapsulated immunomodulator (levamisole) in a mice/larval ascaris suum model provided protection against a challenge infection (2000 eggs) in mice immunised by immobilised antigen. the best results (88.9% protection) were obtained with a combination of two doses of liposome entrapped antigen with leamisole. vaccination with liposome vaccine without modulator was slightly less effective (78.7% protection). a single dos ...	1992	1496793
immunoreactivity to two specific regions of chromogranin a in the nervous system of ascaris suum: an immunocytochemical study.	antisera to a highly conserved region of chromogranin a (sequence keltae) and to a hexapeptide (sequence kgqele) adjacent to the putative c-terminus of pancreastatin, a peptide whose sequence is found within the chromogranin a molecule, have been used to examine the localisation of immunoreactivity (ir) to these peptides in ascaris suum. ir to both peptides was found in the nerve rings and nerve cords. in addition, kgqele-ir was also observed in the pharyngeal neurones and in a network of fibres ...	1992	1409533
distribution of a fluorescent ivermectin probe, bodipy ivermectin, in tissues of the nematode parasite ascaris suum.	a fluorescent derivative of the anthelmintic ivermectin (4''-5,7-dimethyl bodipy proprionylivermectin, referred to hereafter as bodipy ivermectin) was synthesized for an investigation of the distribution of avermectins. injected into adult ascaris suum at doses greater than 0.1 micron per worm, it produced a dose-dependent immobilization. fluorescent microscopy of frozen sections revealed the distribution of the probe in the whole nematode. staining of collagenase-isolated muscle cells was studi ...	1992	1409535
development of an animal model of late asthmatic response in guinea pigs and effects of anti-asthmatic drugs.	we developed an animal model of late asthmatic response (lar) in guinea pigs and examined the effects of anti-asthmatic drugs and peptide leukotriene antagonist, mci-826, on this model. bronchial challenge of dnp-as (dinitrophenylated-ascaris suum extract)-sensitized guinea pigs induced a biphasic increase in pulmonary resistance (rl) with the maximal increase being observed immediately (iar, immediate asthmatic response) and 3 to 5 hr after antigen inhalation (lar). twelve of 22 guinea pigs sho ...	1992	1410517
detection of circulating antigens in human trichinellosis.	a 'sandwich' enzyme-linked immunosorbent assay was established to detect circulating antigens of trichinella spiralis in human sera, its sensitivity and specificity was evaluated using 4 antigens (trichinella spiralis, trichuris trichiura, dirofilaria immitis and ascaris suum), and it was found to be sensitive and specific for t. spiralis antigen. samples of 347 individuals with suspected trichinellosis, who had eaten incompletely cooked bear meat containing larvae of t. spiralis, were examined. ...	1992	1412659
patent infections of ascaris suum in pigs: effect of previous exposure to multiple, high doses of eggs and various treatment regimes.	fifty-four crossbred, 4-week-old pigs divided into nine equal groups were used to test whether multiple inoculations with high numbers of a. suum eggs with or without anthelmintic would result in patent infections. all pigs exposed to multiple prechallenge inoculations of 500, 1000, 2000, 5000, 10,000 and 20,000 and challenged orally 2 weeks later with 10,000 eggs harboured adult worms. when prechallenge infections were removed by pyrantel tartrate treatment the animals were more susceptible to ...	1992	1399243
pharmacological profiles of the gaba and acetylcholine receptors from the nematode, ascaris suum.	the pharmacological profiles of the acetylcholine and gaba receptors of ascaris muscle have been investigated. acetylcholine excites the muscle through activation of a nicotinic receptor which resembles the mammalian ganglionic receptor. dmpp is a potent agonist and alpha-bungarotoxin is a weak antagonist. gaba inhibits the muscle through activation of a chloride-linked receptor which in terms of agonist profile resembles the mammalian gaba-a receptor. however, bicuculline is inactive and picrot ...	1992	1338558
the mitochondrial genomes of two nematodes, caenorhabditis elegans and ascaris suum.	the nucleotide sequences of the mitochondrial dna (mtdna) molecules of two nematodes, caenorhabditis elegans [13,794 nucleotide pairs (ntp)], and ascaris suum (14,284 ntp) are presented and compared. each molecule contains the genes for two ribosomal rnas (s-rrna and l-rrna), 22 transfer rnas (trnas) and 12 proteins, all of which are transcribed in the same direction. the protein genes are the same as 12 of the 13 protein genes found in other metazoan mtdnas: cyt b, cytochrome b; coi-iii, cytoch ...	1992	1551572
ascaris hemoglobin gene: plant-like structure reflects the ancestral globin gene.	animal globin genes have two introns at strictly conserved positions, while plant globin genes have both of these as well as an additional, central intron. it has been proposed that a common ancestor gene had three introns, one of which was subsequently lost from animal but not plant globin genes. we have elucidated the cdna sequence and gene structure of a hemoglobin from the parasitic nematode ascaris suum and found a plant-like central intron, providing strong evidence for a three-intron ance ...	1992	1465385
isoenzymatic pattern and structure of glutamate dehydrogenase from ascaris suum.	the isoenzymatic pattern of glutamate dehydrogenase (gdh) has been described for ascaris suum a parasite of sus scrofa domestica. only one band of activity has been revealed, suggesting a monomorphic condition for this enzyme. also, the structure of gdh has been assayed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and silver staining. only one subunit was present with a molecular weight of about 55,000. a hexameric structure for gdh of a. suum is suggested.	1992	1293199
antigen-induced late-phase airway obstruction in the guinea pig?	hartley or dunkin-hartley guinea pigs (n = 136) were actively sensitized to ovalbumin or ascaris suum protein by five different regimes. specific airway resistance (sr(aw)) was measured in conscious animals by a plethysmographic procedure before, immediately following and at various intervals (up to 96 h) after aerosolized antigen or vehicle challenge. each sensitization and challenge regime produced an immediate allergic response with positive responses (defined as a 2-fold increase in sr(aw)) ...	1992	1295358
lack of late-phase airway responses in conscious guinea pigs after a variety of antigen challenges.	guinea pigs were actively sensitized by parenteral injections of ovalbumin (oa), house dust extract (hd) or ascaris suum extract (as) in a variety of multidose regimens. at least 3 weeks after the initial sensitization injection, aerosols of the appropriate antigen were administered to conscious guinea pigs in a double-chamber body plethysmograph. oa elicited the most consistent and intense bronchoconstriction (bc) as measured by decreases in specific airway conductance (sgaw). the airway respon ...	1992	1295368
immunocytochemical and electrophysiological investigations of central neurones in the parasitic nematode ascaris suum.	here we report on preliminary investigations into the morphological, neurochemical and electrophysiological characteristics of central neurones in the parasitic nematode ascaris suum. the neurones do not display all or none action potentials and apparently have low resting membrane potentials. neurones have been shown to respond to acetylcholine. immunoreactivity to a diverse range of neuropeptides has been demonstrated.	1992	1299113
sk&f 104353, a selective leukotriene receptor antagonist, inhibits leukotriene d4- and antigen-induced bronchoconstriction in cynomolgus monkeys.	the ability of sk&f 104353 to prevent and reverse leukotriene (lt) d4- and antigen (ag)-induced bronchoconstriction was examined in anesthetized, spontaneously breathing cynomolgus monkeys. aerosol administration of ltd4 (10 micrograms/ml; 20 breaths) produced a sustained increase in pulmonary resistance and decrease in dynamic lung compliance. aerosolized sk&f 104353 (150 breaths, 0.3 or 4.4 mg/ml) administered 15 min prior to ltd4 challenge antagonized these changes in a dose-dependent manner. ...	1992	1332792
characterization of the major phosphofructokinase-dephosphorylating protein phosphatases from ascaris suum muscle.	in contrast to the mammalian enzyme, pfk from the nematode ascaris suum is activated following phosphorylation (daum et al. (1986) biochem. biophys. res. commun. 139, 215-221) catalyzed by a camp-dependent protein kinase (thalhofer et al. (1988) j. biol. chem. 263, 952-957). in the present report, we describe the characterization of the major pfk dephosphorylating phosphatases from ascaris muscle. two of these phosphatases exhibit apparent m(r) values of 174,000 and 126,000, respectively, and ar ...	1992	1321672
identification of a novel 5-htn (nematoda) receptor from ascaris suum muscle.	1. the abilities of various serotonergic drugs to bind with the 5-ht receptor of ascaris suum muscle and to affect cyclic amp levels in muscle tissue were examined. 2. ligands which selectively interact with either the 5-ht1 or the 5-ht2 receptor in mammalian systems interact with the 5-ht receptor from a. suum muscle and increase cyclic amp levels. 3. no binding of 5-ht3 ligands to 5-ht receptors from a. suum muscle was observed. 4. the 5-ht receptor of a. suum muscle should be called the 5-htn ...	1992	1354123
chromatographic and immunological characterisation of neuropeptide y-like and pancreatic polypeptide-like peptides from the nematode ascaris suum.	1. immunoreactivity (ir) towards neuropeptide y (npy) and pancreatic polypeptide (pp) has previously been demonstrated in nematodes by immunocytochemistry (icc). 2. the pp- and npy-ir in the nematode parasite ascaris suum were analysed using hplc and radioimmunoassays (rias) specific for either peptide. 3. quantitative ria data on the distribution of these irs in the parasite disagreed with icc data suggesting that different tissue treatments used in the two techniques lead to different antigen ...	1992	1360356
the pyruvate dehydrogenase complex from the parasitic nematode ascaris suum: novel subunit composition and domain structure of the dihydrolipoyl transacetylase component.	the pyruvate dehydrogenase complex (pdc) from muscle of the adult parasitic nematode ascaris suum plays a unique role in its anaerobic mitochondrial metabolism. resolution of the intact complex in high salt dissociates the pyruvate dehydrogenase subunit but leaves the dihydrolipoyl dehydrogenase subunit (e3) and two other proteins with apparent m(r)s of 45 and 43 kda bound to the dihydrolipoyl transacetylase (e2) core. these proteins are not observable on coomassie brilliant blue-stained gels of ...	1992	1376597
a monoclonal antibody-based immunodiagnostic assay for onchocerciasis.	five murine monoclonal antibodies raised against onchocerca volvulus cuticular extracts and termed movs (1-4 and 6) were selected based on reactivity with o. volvulus cryosections, and non-reactivity with cryosections of human skin and/or nodular tissue. two others movs 5 and 7 reacted with both. using the peroxidase-anti- peroxidase (pap) histochemical method, the target epitopes of mov 1 were located in the cuticle's basal and cortical layers, those of mov 2 in the cortical layer; whilst mov 3 ...	1992	1381517
electron-transfer complexes of mitochondria in ascaris suum.	during the past ten years, studies on the respiratory chain of mitochondria in parasites have progressed to provide new insight into the structural organization and physiological significance of the mitochondrial respiratory chain. in this review, kiyoshi kita focuses on studies on the respiratory chain of ascaris mitochondria in which major advances have recently been made. these include the identification of the unique features of anaerobic respiration, the elucidation of the molecular structu ...	1992	15463602
patch-clamp and chloride channels in ascaris suum.	single-channel electrophysiology is an invaluable tool fo the study of ion channels. however, it is a technique that has failed to attract widespread use by parasitologists. here, diane dixon and richard martin outline the principles undelrying single channel recording and highlight its uses in the discovery of a new and unusual chloride channel in the musculature of ascaris suum.	1993	15463802
major sperm protein genes from globodera rostochiensis.	three genes in the major sperm protein (msp) gene family from the potato cyst nematode globodera rostochiensis were cloned and sequenced. in contrast to the absence of introns in caenorhabditis elegans msp genes, these genes in g. rostochiensis contained a 57 nucleotide intron, with normal exon-intron boundaries, in the same relative location as the intron in onchocerca volvulus. the msp genes of g. rostochiensis had putative caat, tata, and polyadenylation signals. the predicted g. rostochiensi ...	1993	19279808
mitochondrial dna sequence divergence among meloidogyne incognita, romanomermis culicivorax, ascaris suum, and caenorhabditis elegans.	mitochondrial dna sequences were obtained from the nadh dehydrogenase subunit 3 (nd3), large rrna, and cytochrome b genes from meloidogyne incognita and romanomermis culicivorax. both species show considerable genetic distance within these same genes when compared with caenorhabditis elegans or ascaris suum, two species previously analyzed. caenorhabditis, ascaris, and meloidogyne were selected as representatives of three subclasses in the nematode class secernentea: rhabditia, spiruria, and dip ...	1993	19279810
immunocytochemical demonstration of neuropeptides in the central nervous system of the roundworm, ascaris suum (nematoda: ascaroidea).	the localization and distribution of neuropeptides in the central nervous system of the pig roundworm, ascaris suum, have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. antisera to 25 vertebrate peptides and two invertebrate peptides were used to screen the worm for immunoreactivity (ir). immunostaining was obtained with antisera to pancreatic polypeptide (pp), peptide yy (pyy), neuropeptide y (npy), gastrin, cholecystokinin (cck), substance ...	1993	7683785
adhesion molecules in acute and chronic lung inflammation.		1993	7524284
use of specific antisera for the localisation and quantitation of leucokinin immunoreactivity in the nematode, ascaris suum.	1. the leucokinins (lks) are a group of eight related peptides isolated from the cockroach, leucophaea maderae. 2. antisera raised against lk-v, which were specific for the conserved mid to c-terminal region of the lks, were used to immunostain the parasitic nematode ascaris suum. 3. lk-ir was observed in neurons in the anterior nerve ring, retrovesicular ganglion, and ventral and dorsal nerve cords of the parasite. immunostaining was specific in that it was abolished by preabsorption of the ant ...	1993	7904924
the aba-1 allergen of the nematode ascaris suum: epitope stability, mass spectrometry, and n-terminal sequence comparison with its homologue in toxocara canis.	aba-1 is a major allergen of nematode parasites of the genus ascaris which includes the large roundworms of humans and pigs, a. lumbricoides and a. suum, respectively. the allergen was purified from a. suum by immunoaffinity chromatography for immunochemical examination. the ige antibody repertoire is under mhc control in infected rodents and the ige-binding epitopes were robust to treatment with heat or periodate, and electroblotting on nitrocellulose. this implies that the ige epitopes compris ...	1993	7682160
inhibition of antigen-induced airway and cutaneous responses by heparin: a pharmacodynamic study.	we have previously shown that heparin attenuates the acute bronchoconstrictor response and immediate cutaneous reaction (icr) to antigen in allergic sheep. in the present investigation, we studied the pharmacodynamics of the antiallergic action of heparin. specific lung resistance (srl) was measured in eight sheep, allergic to ascaris suum antigen, before and 5 min after inhalation challenge with the antigen. on different experiment days, antigen challenge was repeated after pretreatment with 1) ...	1993	7685753
characterization of cdna clones for the 2-methyl branched-chain enoyl-coa reductase. an enzyme involved in branched-chain fatty acid synthesis in anaerobic mitochondria of the parasitic nematode ascaris suum.	the 2-methyl branched-chain enoyl-coa reductase plays a pivotal role in the reversal of beta-oxidation operating in anaerobic mitochondria of the parasitic nematode ascaris suum. an affinity-purified polyclonal anti-serum against the reductase was used to screen a cdna library constructed in lambda gt11 with poly(a)+ rna from adult a. suum muscle. a 1.2-kilobase partial cdna clone was isolated, subcloned, and sequenced in both directions. additional sequence at the 5' end of the mrna was determi ...	1993	7693666
a patch-clamp study of the ionic selectivity of the large conductance, ca-activated chloride channel in muscle vesicles prepared from ascaris suum.	plasma membrane vesicles prepared from the bag region of the somatic muscle cell of the parasite ascaris suum contain a large conductance, voltage-sensitive, calcium-activated chloride channel. the ability of this channel to conduct a variety of anions has been investigated using the patch-clamp technique on isolated inside-out patches of muscle membrane. symmetrical cl solutions (140 mm) produced single-channel i/v plots with reversal potentials of 0 mv, substitution of bath cl by 140 mm no3, b ...	1993	7680073
[in vitro study of anthelmintic influence on activities of digestive enzymes in extracts from the gut of ascaris suum and pig's pancreas].	the effect of eight anthelmintics (rintal, fenbesan, telmin, banminth, pyrantel, nilverm, levamisol and bioscardina) on the alpha- and gamma-amylases, trypsin, and lipase from pig's pancreas and gut of ascaris suum was determined. in extracts from a. suum gut also the maltase, trehalase and saccharose activities were examined. all drugs tested did not influence on the host's alpha-amylase. telmin and bioscardina were inhibitors of gamma-amylase, rintal and telmin--of trypsin, fenbesan and biosca ...	1993	8128727
characterization and biological activities of anti-brugia pahangi tubulin monoclonal antibodies.	three monoclonal antibodies (mab) specific to beta-tubulin were used to investigate the heterogeneity of tubulins from nematodes and mammals. western blot analysis of one-dimensional sds-page showed that anti-brugia pahangi tubulin mab 1b6 and p3d react with epitope(s) specific to nematode beta-tubulin and recognize tubulin from adults and microfilariae of b. pahangi, adult b. malayi and dirofilaria immitis, eggs of haemonchus contortus and adult ascaris suum. however, the same mab did not recog ...	1993	8106183
mechanisms of bronchoconstriction after allergen ingestion in sensitized guinea pigs.	we examined whether oral administration of allergen induced bronchoconstriction in sensitized guinea pigs and investigated the mechanisms of bronchoconstriction. the animals had been immunized intraperitoneally with a mixture of ascaris suum extract and silica gel, and exposed to ozone. they were then challenged with an oral dose of a. suum extract (6 mg/kg), and respiratory resistance (rrs) was measured up to 7 h. after oral administration of the allergen, an increase in rrs was observed. the m ...	1993	8219783
isolation of porcine ige, and preparation of polyclonal antisera.	high titre, ascaris specific, porcine reaginic antibody was induced by combining parasitic infestation and antigen challenge. the reagin was characterised as being heat labile at 56 degrees c, possessing long term skin sensitising activity, and as having physicochemical properties similar to ige in other species. porcine ige was purified by conventional immunochemical techniques, and an epsilon chain isolate prepared using sds-page gel excision and electro elution. antisera raised against the in ...	1993	8236796
levamisole-activated single-channel currents from muscle of the nematode parasite ascaris suum.	1. the patch-clamp technique was used to examine levamisole-activated channels in muscle vesicles from ascaris suum. cell-attached and isolated inside-out patches were used. 2. levamisole (1-90 microm), applied to the extracellular surface, activated channels which had apparent mean open-times in the range 0.80-2.85 ms and linear i/v relationships with conductances in the range 19-46 ps. ion-replacement experiments showed the channels to be cation selective. 3. the kinetics of the channels were ...	1993	7679027
comparison of three enzyme immunoassays for diagnosis of dictyocaulus viviparus infection.	three enzyme-linked immunosorbent assays (elisa) that detect antibodies against dictyocaulus viviparus in cattle were compared for sensitivity, specificity and seroconversion after primary infection. these assays were (i) an indirect elisa with crude somatic antigens from adult d. viviparus (ca-elisa), (ii) an indirect elisa with purified antigens (sa-elisa) isolated from somatic antigens of adult d. viviparus and (iii) a competition elisa with antigen purified with anion chromatography in combi ...	1993	8249247
an ion-sensitive microelectrode study on the effect of a high concentration of ivermectin on chloride balance in the somatic muscle bag cells of ascaris suum.	ivermectin has been shown to increase chloride conductances of invertebrate cells. on the muscle cells of the parasitic nematode ascaris, ivermectin acts as both a gaba receptor antagonist and a chloride channel opener. in this study, ion-sensitive microelectrodes were used to investigate the effect of ivermectin on intracellular cl- concentration of the somatic muscle bag cells of ascaris suum. incubation of muscle cells with ivermectin (10 microm in 1% dimethyl sulphoxide vehicle for 60 min) i ...	1993	8316438
classification of fumarate reductases and succinate dehydrogenases based upon their contrasting behaviour in the reduced benzylviologen/fumarate assay.	reduction of fumarate by soluble beef heart succinate dehydrogenase has been shown previously by voltammetry to become increasingly retarded as the potential is lowered below a threshold potential of -80 mv at ph 7.5. the behaviour resembles that of a tunnel diode, an electronic device exhibiting the property of negative resistance. the enzyme thus acts to oppose fumarate reduction under conditions of high thermodynamic driving force. we now provide independent evidence for this phenomenon from ...	1993	8325393
novel gene structure and evolutionary context of caenorhabditis elegans globin.	animal and plant globin-encoding genes (glo) contain two introns in strictly conserved positions. plant glo genes possess an additional, centrally located intron. we have determined the cdna sequence and gene structure of a putative glo gene from the free-living nematode, caenorhabditis elegans. the gene encodes a one-domain globin with a single intron, corresponding to the central intron of plant glo genes. the two introns common to virtually all animal and plant glo genes are missing. comparis ...	1993	8325507
histological changes in hamster liver by es antigen of ascaris suum.	polyacrylamide gel electrophoresis with sds (page-sds) of the es antigens of a. suum revealed several protein molecules which differed from those obtained in es antigens of a. lumbricoides. nature of liver damage caused by es antigens of a. suum was studied in hamsters to find out the nature of damage and to compare with those caused by es antigens of a. lumbricoides. feeding of es antigens of a. suum was carried out in 7 hamsters for 75 days. after such feeding gross hepatic damage was noticed. ...	1993	8262573
doramectin, a new avermectin highly efficacious against gastrointestinal nematodes and lungworms of cattle and pigs: two studies carried out under field conditions in germany.	two field studies were carried out to confirm the efficacy of doramectin in cattle and pigs in europe. a total of 40 cattle harbouring naturally acquired infections of gastrointestinal nematodes, including ostertagia spp., oesophagostomum spp., cooperia spp., haemonchus spp., nematodirus spp. and trichuris spp., were allocated to a treated or a control group (20 animals/group), and 100 harbouring naturally acquired infections of gastrointestinal nematodes and lungworms, including oesophagostomum ...	1993	8278344
immediate allergic and nonallergic reactivity of the airways in ascaris skin-sensitive sheep.	immediate airway reactivity to ascaris suum allergen was compared with the responses to nonallergic airway challenge using histamine and acetylcholine in sheep with positive skin reactions to the allergen. in a control group of 6 sheep with negative skin test responses, allergen aerosol inhalation and allergen extract infusion only caused minor changes in mechanics of breathing as well as in arterial plasma histamine concentration. nearly 40% of the animals with a positive skin test responded to ...	1993	8284521
isolation and preparation of antisera to ovine ige.	elevated levels of ovine reaginic antibody were induced by immunization with ascaris suum antigens. the pca activity of this antibody persisted in the skin of sheep for 20 days and was abolished by heating to 56 degrees c, suggesting that it was immunoglobulin e. attempts to isolate ige from this hyperimmune serum by gel filtration, ion exchange and affinity chromatography resulted in the preparation of a pca-positive fraction containing proteins with molecular weights of 70, 56 and 22 kd on sds ...	1993	8353460
the diagnostic antigen encoded by gene fragment dv3-14: a major sperm protein of dictyocaulus viviparus.	the dv3-14 gene fragment of dictyocaulus viviparus is 471 bp, of which about 90% is translated. the calculated molecular mass of the translation product is 15.5 kda. dna sequence homologies were found with major sperm proteins (msps) from ascaris suum, caenorhabditis elegans and onchocerca volvulus. within a segment of 62 amino acids the predicted amino acid sequence of dv3-14 shows 84% homology with an a. suum msp and 84% homology with a c. elegans msp. computer analysis of the protein sequence ...	1993	8359987
ascaris suum: cloning of a cdna encoding phosphoenolpyruvate carboxykinase.	a cdna encoding phosphoenolpyruvate carboxykinase (pepck) from ascaris suum was cloned by complementation of a strain of escherichia coli deficient in pepck and malic enzyme. the product of this cdna was enzymatically similar to a recombinant pepck obtained from haemonchus contortus by the same method. comparison of the predicted amino acid sequence of a. suum pepck with other pepcks showed that this enzyme is most closely related to the h. contortus enzyme. the two nematode enzymes share consid ...	1993	8375484
mitochondrial heterogeneity in the parasitic nematode, ascaris suum.	the anaerobic metabolism of ascaris suum body wall muscle mitochondria has been well characterized, but little is known about the metabolism of other adult tissues. the present study was designed to further characterize the metabolism of mitochondria isolated from a. suum male reproductive tissues, which contain predominately sperm, and to compare it with that of muscle. cytochrome oxidase activity could not be detected in muscle, testis, or sperm mitochondria either by diaminobenzidine staining ...	1993	8390371
[alpha-amylase release by pancreatic acini isolated from guinea pigs infected with invasive eggs of ascaris suum].	the studies were carried out on dispersed acini prepared from pancreas of guinea pigs infected with 5000 invasive eggs of ascaris suum. the basal amylase secretion and that stimulated with acetylcholine and carbachol or blocked with atropine was determined. the basal secretion of amylase was significantly higher in pancreatic acini isolated from infected animals. the stimulation by acetylcholine and carbachol of these acini was weaker than in the control group. these results suggest that choline ...	1993	8122421
the filarial antigens av33/ov33-3 show striking similarities to the major pepsin inhibitor from ascaris suum.		1993	8433724
use of body fluid of adult female ascaris suum as an antigen in the enzyme-linked immunosorbent assay (elisa) for diagnosis of swine ascariosis.	the enzyme-linked immunosorbent assay (elisa) was used to detect ascaris suum antibodies in swine sera with adult body fluid (abf) as an antigen. the assay was standardized with respect to the antigen concentration and serum and conjugate dilutions. cross reaction was found between the antigen and the sera from the swine infected with metastrongylus apri. the elisa was more sensitive than the complement fixation test. five protein peaks were obtained from abf by gel filtration on sephacryle s-30 ...	1993	8132972
the development and evolution of actin-containing organelles during spermiogenesis of a primitive nematode.	spermatogenesis in the primitive marine nematode sphaerolaimus hirsutus (chromadoria, sphaerolaimidae) was investigated by examining the ultrastructure and cytochemistry. spermatozoa are lenticular cells of about 15 microns in diameter and are devoid of flagellum and acrosome as in other nematodes. in spermatocytes, dictyosomes produced transient structures, the fibrous body-membranous organelle complexes (fb-mo). in spermatids, the fbs were arranged as cartwheel spokes, with the fbs in the cent ...	1993	8004009
inhibition of ige-mediated leukotriene generation and bronchoconstriction in primates with a new 5-lipoxygenase inhibitor, e6080.	the inhibitory effects of a novel compound, 6-hydroxy-2-(4-sulfamoylbenzylamino)-4,5,7-trimethylbenzothiazo le hydrochloride (e6080) on ige-mediated reactions in vitro and in vivo were determined in rhesus monkeys. e6080 inhibited both antigen- and anti-human ige-induced leukotriene c4 generations from lung fragments at similar concentrations: the ic50s of e6080 were 1.11 and 0.96 microm, respectively. aa861 also inhibited both leukotriene c4 generations with ic50s of 0.79 and 0.76 microm, respe ...	1993	8443470
[the effect of experimental aerobic stabilization of swine slurry on survival of salmonella typhimurium and ascaris suum].	laboratory aerobic mesophilic stabilisation (fermentation) of pig slurry reduced the survival time of s. typhimurium, compared with their prevalence in anaerobic excrements. the decimation time t90 for s. typhimurium, was 32 hours. the effect of aerobic stabilisation on the survival of nonembryoed eggs of the model helminth a. suum was lower than that observed in salmonella. after 54 hours of aerobic stabilization of slurry only 32% of eggs were devitalized. stabilized control group did not deve ...	1993	8236636
inhibition of antigen-induced airway hyperresponsiveness by a thromboxane a2 receptor antagonist (aa-2414) in ascaris suum-allergic dogs.	we studied changes in airway responsiveness to acetylcholine (ach) after antigen inhalation in ascaris suum (a. suum)-allergic dogs. airway responsiveness was determined by obtaining a dose-response curve of lung resistance plotted against increasing concentrations of ach aerosol before and after inhalation of a. suum antigen. to determine the role of thromboxane a2 (txa2) in the airway response, we tested the effect of a txa2 receptor antagonist, aa-2414, in a. suum-allergic dogs. the procedure ...	1993	8248544
inhalation of platelet-activating factor increases respiratory resistance in rats: determination by means of an astograph under nonanesthetized conditions.	intratracheal administration of platelet-activating factor (paf) to dogs, baboons, and humans has been shown to induce hyperreactivity of the airways and contraction of the smooth muscle. however, it has not yet been reported whether intratracheal administration of paf to rats induces hyperreactivity. in the present study, the authors estimated the respiratory resistance of rats during intratracheal administration of paf in order to evaluate the reactivity of the airways to paf. in both the nons ...	1993	8459752
ecdysteroids during development in the ovine parasitic nematode, haemonchus contortus.	1. samples of embryonating eggs, infective larvae, fourth-stage larvae, young adults, and male and female mature adults of haemonchus contortus were collected for steroids analysis; lipids were extracted, partially purified, fractionated with hplc, and analyzed for ecdysteroids by radioimmunoassay. 2. free ecdysone and 20-hydroxyecdysone were detected in all samples; maximal concentrations occurred in the fourth-stage larvae and young adults. 3. conjugated ecdysone and 20-hydroxyecdysone were de ...	1993	8472534
isoenzymatic pattern of glucose 6-phosphate dehydrogenase from ascaris suum.	the isoenzymatic pattern of glucose 6-phosphate dehydrogenase (abbreviation g6pd) from ascaris suum has been studied by vertical polyacrylamide gel (page) and horizontal starch gel electrophoresis. after polyacrylamide gel electrophoresis, two stained zones could be identified. one corresponded to tetrazolium oxidase activity; since this zone was stained even in the absence of glucose 6-phosphate (g6p), non-specific staining could be detected. in the other zone of activity, seven regularly-space ...	1993	8288854
immunocytochemical demonstration of neuropeptides in the peripheral nervous system of the roundworm ascaris suum (nematoda, ascaroidea).	the localisation and distribution of neuropeptides in the peripheral nervous system of the pig roundworm ascaris suum have been determined by an indirect immunofluorescence technique in conjunction with confocal microscopy. of the 31 antisera tested, immunostaining was obtained only with antisera to peptide yy (pyy), pancreatic polypeptide (pp) and fmrfamide. immunostaining for pyy and fmrfamide was evident in the amphidial and papillary ganglia associated with the anterior nerve ring and in the ...	1993	8327453
crystallization of the motile major sperm protein (msp) of the nematode ascaris suum.	we have obtained orthorhombic crystals (space group p2(1)2(1)2(1), a = 72 a, b = 78 a, c = 452 a) of the major cytoskeletal protein associated with the amoeboid motility of ascaris sperm. these crystals diffract past 3.5 a and appear to be constructed from arrays of helical fibres of the major sperm protein. the fibres within the crystals appear to be closely analogous to those seen in vitro and in vivo.	1993	8331665
af2, an ascaris neuropeptide: isolation, sequence, and bioactivity.	a fmrfamide-like neuropeptide, kheylrfamide (lys-his-glu-tyr-leu-arg-phe-amide; af2) was isolated from a head extract of the nematode ascaris suum by using a three-step hplc separation. in a dorsal muscle strip preparation, synthetic af2 produced multiple effects on muscle tension: a slow relaxation was followed by contraction and rhythmic activity. sulfated af2 was no more potent than af2. the effects on muscle tension were correlated with electrical activity recorded intracellularly from muscl ...	1993	8332542
effects of heavy metals imission on the cellular immunity of guinea pigs with experimental ascariosis.	a study was performed on the short-term effects of industrial heavy metals imission (hg, cu, pb, zn), collected at 5 km from a mercury-producing plant, on t- and b-lymphocytes and peritoneal macrophages of guinea pigs with experimental ascariosis (ascaris suum). administration of the imission for 5 days showed a significant decrease in t- and b-cell population and in macrophage phagocytic ability during the migration phase of ascaris suum when compared with an infected but untreated group of ani ...	1993	8333130
[the helminth fauna of wild boars of the west berlin forests].	samples of muscles and internal organs obtained from 96 wild boars in the forests of west berlin between september 1989 and august 1990, were examined for helminths. nine nematode species, metastrongylus pudendotectus, m. salmi, m. confusus, m. apri, physocephalus sexalatus, ascarops strongylina, globocephalus urosubulatus, ascaris suum and trichuris suis belonging to five families could be found. larvae of toxocara spp. were isolated from most of the enzymatically digested samples of the gastro ...	1993	8343108
kinetics of ligand binding to pseudoterranova decipiens and ascaris suum hemoglobins and to leu-29-->tyr sperm whale myoglobin mutant.	the kinetics of binding of o2, co, and no to the octameric, two-domain hemoglobins of the parasitic nematodes pseudoterranova decipiens and ascaris suum were determined on nanosecond and picosecond time scales using flash photolysis. the two nematode hemoglobins have very similar kinetic properties. on the picosecond time scale, they exhibit an unusual behavior in showing a geminate reaction with oxygen that is biphasic and dependent on the flash intensity. the geminate reaction with no is also ...	1993	8349589
expression, characterization, and crystallization of oxygen-avid ascaris hemoglobin domains.	the ascaris perienteric hemoglobin is 10(4) times more oxygen-avid than mammalian hemoglobins. inspection of its primary structure fails to explain this extraordinary association with oxygen. the ascaris hemoglobin gene encodes a 40-kda, two-domain globin; the two domains (d1 and d2) are 63% identical, and each is capable of binding a single heme. the native protein is an octamer. at the end of d2 is a highly charged carboxyl-terminal extension containing four direct repeats of hkee. we have exp ...	1993	8349648
localization and differential expression of fmrfamide-like immunoreactivity in the nematode ascaris suum.	by immunocytochemical and immunohistochemical methods, fmrfamide-like immunoreactivity (fli) was localized to many neurons and processes in the ascaris nervous system, including the head, tail, and lateral lines. some of these cells were identified; they included sensory neurons, interneurons, and motor neurons. fli was also present in the pharyngeal neurons and in their varicosities near the surface of the pharynx. by hplc analysis of extracts, only a subset of the fmrfamide-like peptides (flps ...	1993	8349852
purification and characterization of electron-transfer flavoprotein: rhodoquinone oxidoreductase from anaerobic mitochondria of the adult parasitic nematode, ascaris suum.	electron-transfer flavoprotein:rhodoquinone oxidoreductase (etf-ro) was purified to homogeneity from anaerobic mitochondria of the parasitic nematode, ascaris suum. the enzyme has a subunit molecular mass of 64.5 kda and is similar in many respects to the electron-transfer flavoprotein:ubiquinone oxidoreductase (etf-uo) characterized in mammalian tissues. epr spectroscopy of the purified enzyme revealed signals at g = 2.076, 1,936, and 1.883, arising from an iron-sulfur center, as well as signal ...	1993	8376393
adjuvant effect of interleukin-1 on the development of late asthmatic response in guinea pigs.	the adjuvant effect of silica and il-1 in the development of late asthmatic responses (lars) in guinea pigs was studied. different doses of silica or recombinant human (rh) il-1 (1 microgram) with ascaris suum were used for the immunization. the serum il-1 concentration was measured after the immunization. one week after the immunization, antigen was challenged and respiratory resistance (rrs) was measured. rrs increased silica dose dependently in the late phase, and the increment of rrs in the ...	1993	8382978
potentiation of ige responses to third-party antigens mediated by ascaris suum soluble products.	a reductive approach was used to examine the potentiation of ige responses by nematode infection. ascaris homogenized extract, ascaris pseudocoelomic (body) fluid (abf) and purified ascaris allergen (aba) were tested for their ability to act as protein carriers and as mediators of potentiated ige responses to third-party (ovalbumin; ova) responses. all three nematode products were excellent protein carriers for the hapten dinitrophenol and showed significantly better activity in this respect tha ...	1993	8400897
oxygen radicals contribute to antigen-induced airway hyperresponsiveness in conscious sheep.	we previously showed that oxygen radicals can induce airway hyperresponsiveness (ahr) in allergic sheep. the purpose of this study was to determine whether antigen challenge results in the generation of free oxygen radicals and if these radicals contribute to antigen-induced ahr. we first determined baseline airway responsiveness in seven ascaris suum-sensitive sheep by calculating the cumulative provocative concentration of carbachol in breath units (bu; one bu defined as one breath of a 1% wt/ ...	1993	8430955
developmental changes in the respiratory chain of ascaris mitochondria.	the ascaris larval respiratory chain, particularly complex ii (succinate-ubiquinone oxidoreductase), was characterized in isolated mitochondria. low-temperature difference spectra showed the presence of substrate-reducible cytochromes aa3 of complex iv, c+c1 and b of complex iii (ubiquinol-cytochrome c oxidoreductase) in mitochondria from second-stage larvae (l2 mitochondria). quinone analysis by high-performance liquid chromatography showed that, unlike adult mitochondria, which contain only rh ...	1993	8435436