Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| toenail onychomycosis in estonia. | the aim of our study was to evaluate the clinical features, predisposing factors and pathogens of toenail onychomycosis in estonia. during study period we interviewed and examined 436 dermatological patients with clinical signs of toenail onychomycosis in all counties of estonia. in 69% of cases, the clinical diagnosis of onychomycosis was confirmed by the mycological analysis. the most common clinical symptoms of onychomycosis both in mycologically proven and non-proven cases were discolorizati ... | 2004 | 14998401 |
| trichophyton rubrum in the external auditory meatus. | we report the case of a 28-year-old immunocompetent male suffering from otitis externa. the right external auditory meatus was filled with cerumen and detritus, the tympanic membrane covered wallpaper-like with layers of fungi. mycological analysis revealed trichophyton rubrum. with further examination tinea pedis of plantar and interdigital type and concomitant onychomycosis of the toenails due to t. rubrum could be detected. the auditory meatus was cleaned and treated topically with clotrimazo ... | 2004 | 14998407 |
| antidermatophytic activity of extracts from psoralea corylifolia (fabaceae) correlated with the presence of a flavonoid compound. | extracts obtained from seeds of psoralea corylifolia showed several degrees of antifungal activity against trichophyton rubrum, trichophyton mentagrophytes, epidermophyton floccosum and microsporum gypseum by the disc diffusion method on a sabouraud dextrose agar (sda) medium. methanol extract of the seeds at 250 microg exhibited the maximum inhibition with a halo of 28 mm diameter. six different bands were obtained when the methanol extract was subjected to tlc. 13c nmr and mass spectra reveale ... | 2004 | 15036462 |
| antibacterial and antifungal activity of syzygium jambolanum seeds. | the water and methanolic extracts of syzygium jambolanum seeds were examined for antibacterial and antifungal activity in vitro using the disc diffusion method, minimum inhibitory concentration, minimum bactericidal concentration and minimum fungicidal concentration. activity against gram positive bacteria (bacillus subtilis, staphylococcus aureus), gram negative bacteria (salmonella typhimurium, pseudomonas aeruginosa, klebsiella pneumoniae and escherichia coli) and fungal strains (candida albi ... | 2004 | 15036477 |
| in vitro susceptibility testing of amorolfine in pathogenic fungi isolated from dermatomycosis patients in china. | the antifungal susceptibility of isolates from chinese dermatomycosis patients to amorolfine was investigated following national committee for clinical laboratory standards (nccls) protocols. in total, 383 isolates were tested, including 132 strains from tinea pedis, 148 strains from tinea corporis/cruris, and 103 strains from onychomycosis. the minimum inhibitory concentration (mic) of amorolfine against dermatophytes ranged from 0.01 to 0.08 microg ml(-1). the mic(50) and mic(90) of amorolfine ... | 2004 | 15504124 |
| trichoderma spp. antagonism to the dermatophyte trichophyton rubrum: implications in treatment of onychomycosis. | onychomycosis--the dermatophytic invasion of the nail--is difficult to eradicate with drug treatment. the hyphae of the main invading pathogen, trichophyton rubrum, are often interwoven with the nail plate, preventing effective anti-mycotic agents from reaching its growing tips. an alternative approach to treat onychomycosis may possibly be the application of a biological control agent against the pathogen. in analogy with the success of biocontrol of phytopathogenic fungi, we screened a series ... | 2004 | 15518345 |
| onychomycosis in cali, colombia. | this study presents the epidemiological and mycological aspects of 299 patients with nail lesions who were referred to three diagnostic laboratories in the city of cali. the diagnosis of mycoses was established through visualization of mycotic structures in a direct microscopic examination of skin scrapings and by isolation. onychomycosis was found in 183 cases (61.2%), of which 141 were in toenails (44 in males and 97 in females), 38 in fingernails (9 males and 29 females), and 4 cases in toena ... | 2004 | 15518346 |
| low-voltage direct current as a fungicidal agent for treating onychomycosis. | onychomycosis, most commonly caused by two species of dermatophyte fungi--trichophyton rubrum and trichophyton mentagrophytes--is primarily treated with regimens of topical and systemic antifungal medications. this study was undertaken to evaluate in vitro the efficacy of low-voltage direct current as an antifungal agent for treating onychomycosis. agar plate cultures of t rubrum and t mentagrophytes were subjected to low-voltage direct current electrostimulation, and antifungal effects were obs ... | 2004 | 15547124 |
| antifungal activities of essential oils from thymus quinquecostatus and t. magnus. | the antifungal activities of essential oils from thymus quinquecostatus and t. magnus, which are species native to korea, were evaluated against seven common pathogenic fungi. additionally, the effects of the oils together with ketoconazole were tested by the checkerboard titer test. both of the two thymus oils showed significant inhibition of the tested fungi, with minimal inhibitor concentrations (mics) and minimal fungicidal concentrations (mfcs) in the range of 0.04-0.39 mg/ml and 0.19-0.78 ... | 2004 | 15549669 |
| morphological evidences for onion-induced growth inhibition of trichophyton rubrum and trichophyton mentagrophytes. | the antifungal activity of onion (allium cepa l.) on two important dermatophytes, trichophyton rubrum and trichophyton mentagrophytes, with special reference to morphological aspects was studied. growth of both fungi was found to be strongly inhibited by aqueous onion extract (aoe) as a dose-dependent manner. the extract showed fungicidal effect for both fungi at concentrations >3.12% (v/v). the fungus t. mentagrophytes was more affected by the onion as compared to t. rubrum at all concentration ... | 2004 | 15567239 |
| deep dermatophytosis caused by trichophyton rubrum with concomitant disseminated nocardiosis in a renal transplant recipient. | deep dermatophytosis and nocardiosis are uncommon infections. this article describes a patient who was immunosuppressed with deep dermatophytosis caused by trichophyton rubrum and concurrent disseminated nocardiosis. the infections occurred 16 years after the patient underwent renal transplantation, and may have been related to tacrolimus therapy. | 2004 | 15577763 |
| antibacterial and xanthine oxidase inhibitory cerebrosides from fusarium sp. ifb-121, an endophytic fungus in quercus variabilis. | two antibacterial and xanthine oxidase inhibitory cerebrosides, one of which is chemically new, were characterized from the chloroform-methanol (1:1) extract of fusarium sp. ifb-121, an endophytic fungus in quercus variabilis. by means of chemical and spectral methods [ir, electrospray ionization ms (esi-ms), tandem esi-ms, 1h and 13c nmr, distortionless enhancement by polarization transfer, cosy, heteronuclear multiple-quantum coherence, heteronuclear multiple-bond correlation, and 2-d nuclear ... | 2004 | 15588024 |
| dermatophytes in the rural area of duzce, turkey. | a total of 227 subjects (112 were male and 115 female), who were residents of the rural region of duzce, were examined for dermatomycosis. a total of 120 samples (47 nail fragments, 73 skin scales) were collected from 81 patients for mycological analysis on the basis of the results of clinical evaluation. all specimens collected were analysed by direct microscopy and culture. positive results were detected in the cultures of 53 (44.1%) of the 120 samples. about 46 (86.7%) samples also presented ... | 2004 | 15601451 |
| bullous tinea pedis in two children. | tinea pedis is a dermatophytic infection with a high prevalence in adults and also occasionally observed in children. we report here two cases of bullous tinea pedis, one due to trichophyton rubrum in a 6-year-old child and the second due to t. interdigitale in a 10-year-old child. we suggest that, despite the low prevalence of this infection in childhood, a potassium hydroxide test and a culture for fungi should always be performed when a child is examined for an inflammatory eruption of the fe ... | 2004 | 15601452 |
| [dermatophytoses due to microsporum gypseum: report of eight cases and literature review]. | microsporum gypseum is a geophilic fungus infrequent agent of human dermatophytoses and world-wide in distribution. in cadiz, spain, between 1997 and 2003, a study of 133 positive cases showed that the fifth more isolated dermatophyte was m. gypseum (6.0%), followed by trichophyton mentagrophytes (24,8%), microsporun canis (24,6%), trichophyton rubrum (21,8%) y trichophyton violaceum (6,8%). during 2003 the infection due to this fungus has been repeatedly observed in our area (17.5%). we report ... | 2004 | 15709790 |
| [prevalence and clinical aspects of superficial mycosis in hospitalized diabetic patients in tunisia]. | the prevalence of mycotic infection seems to be higher among diabetic patients than in the non-diabetic population. the aims of this study were to determine the frequency of mycosis and to compare clinical and fungal results. | 2004 | 16235595 |
| importance of mycological confirmation of clinically suspected cases of tinea corporis, tinea pedis and tinea cruris. | tinea corporis, tinea cruris, and tinea pedis are of the most prevalent dermatophytoses. several conditions that mimic dermatophytoses and atypical and steroid modified forms of the disease usually present difficulties in diagnosis. hence, the present investigation aimed at studying these conditions on mycological basis. the study included 163 cases clinically diagnosed as having tinea corporis, tinea pedis or tinea cruris. specimens were taken by skin scraping. samples were cultured on sabourau ... | 2004 | 16916049 |
| the influence of surface carbohydrates during in vitro infection of mammalian cells by the dermatophyte trichophyton rubrum. | in order to better understand the role played by surface glycoconjugates during host cell adhesion and endocytosis of trichophyton rubrum, we looked for the presence of carbohydrate-binding adhesins on the microconidia surface and their role on cellular interaction with epithelial and macrophages cells. the interaction of t. rubrum with chinese hamster ovary epithelial cells and their glycosylation-deficient mutants demonstrated a higher adhesion index in lec1 and lec2 mutants, that express mann ... | 2004 | 15059626 |
| pharmacokinetic investigation of oral itraconazole in stratum corneum level of tinea pedis. | in the present study, the authors administered 100 mg itraconazole (itcz) twice daily for a period of 1 week to six patients with hyperkeratotic type tinea pedis, and examined its efficacy, safety profile, and usefulness. itcz concentration in stratum corneum was also measured to examine the mobility of the drug into the affected site of planta pedis. itcz concentration in the stratum corneum of the affected part was first detected at 1 week after the completion of administration, gradually incr ... | 2004 | 15078426 |
| cloning and functional expression of a mungbean defensin vrd1 in pichia pastoris. | it was shown previously that a bacterially expressed mungbean defensin vrcrp exhibited both antifungal and insecticidal activities. to isolate this protein in a large quantity for its characterization, the defensin cdna was expressed in pichia pastoris and the recombinant defensin (rvrd1) was purified. the recombinant vrd1 was shown to inhibit the growth of fungi such as fusarium oxysporum, pyricularia oryza, rhizoctonia solani, and trichophyton rubrum and development of bruchid larva. the prote ... | 2004 | 15080630 |
| superficial mycoses and hiv infection in yaounde. | the incidence of superficial infections in patients with human immunodeficiency virus (hiv) is increasing in yaounde. to determine the prevalence of superficial mycoses in hiv-positive subjects, a study was carried out in different hospital settings in yaounde over a period of 18 months. clinical observations were followed by the culture of samples on sabouraud's glucose agar. of the 148 hivab-positive patients examined, 79 (44 males and 35 females) had at least one superficial mycosis (53%). th ... | 2004 | 15096139 |
| epidemiologic surveillance of cutaneous fungal infection in the united states from 1999 to 2002. | cutaneous fungal infections are common in the united states, and causative organisms include dermatophytes, yeasts, and nondermatophyte molds. these organisms are in constant competition for their particular environmental niche, often resulting in the emergence of one or more predominant pathogens and displacement of other less competitive species. changes in the incidence of fungal pathogens can be followed from laboratory culture results of infected cutaneous tissues over time. these data can ... | 2004 | 15097959 |
| a comparative survey of serum androgenic hormones levels between male patients with dermatophytosis and normal subjects. | fungal growth can be influenced by human physiological mediators such as androgenic hormones. the aim of this study was to investigate a possible relationship between androgenic hormones and susceptibility to dermatophytosis. to this purpose we measured the levels of testosterone, androstendione, and dehydroepianderosterone sulfate (dhea-s) in 60 male patients with dermatophytosis due to epidermophyton floccosum and trichophyton rubrum by enzyme link immunoassay. serum testosterone concentration ... | 2004 | 15118212 |
| antifungal drug response in an in vitro model of dermatophyte nail infection. | despite terbinafine being fungicidal against trichophyton rubrum in standard nccls assays and rapidly accumulating in nails in vivo, onychomycosis patients require prolonged terbinafine treatment to be cured. to investigate this, we developed a more clinically relevant onychomycosis in vitro test model. human nail powder inoculated with t. rubrum and incubated in liquid rpmi 1640 salt medium, which did not support growth alone, developed extensive and invasive mycelial growth. antifungal drugs w ... | 2004 | 15124869 |
| prevalence of fungal foot infections in patients with diabetes mellitus type 1 - underestimation of moccasin-type tinea. | in diabetic patients, mycotic infections may increase the risk of developing diabetic foot syndrome. however, few data are available on the prevalence of fungal foot infections in patients with diabetes. during a conference attended by patients with long-term diabetes, 95 individuals with type 1 diabetes mellitus (52 men, 43 women, mean disease duration 35.8 years) were examined for fungal infections of the feet. as well as frequency of infection and risk profiles, the level of patient awareness ... | 2004 | 15146373 |
| severe generalized deep dermatophytosis due to trichophyton rubrum (trichophytic granuloma) in a patient with atopic dermatitis. | 2004 | 15148118 | |
| agar sublimation test for the in vitro determination of the antifungal activity of morpholine derivatives. | we studied the in vitro antifungal activities of a wide range of antimycotic agents, including amorolfine, terbinafine, naftifine, five morpholine derivatives, ciclopiroxolamine, bifonazole, clotrimazole, ketoconazole, itraconazole, fluconazole, voriconazole, flucytosine, amphotericin b, nystatin, and caspofungin, against candida albicans and trichophyton rubrum by conventional agar diffusion tests and by a novel sublimation method. for the sublimation method, 6 mm filter paper disks were soaked ... | 2004 | 15189181 |
| the successful treatment of trichophyton rubrum nail bed (distal subungual) onychomycosis with intermittent pulse-dosed terbinafine. | the standard treatment of trichophyton rubrum nail bed onychomycosis (or distal subungual onychomycosis [dso]) with daily terbinafine for 12 weeks involves treating for a fixed period shorter than the time required for complete replacement of the nail bed and overlying nail plate by normal growth. the same total amount of terbinafine pulse-dosed for approximately 12 months would treat the patient until normal replacement of the mycotic nail bed has occurred. | 2004 | 15210459 |
| white superficial onychomycosis: epidemiological, clinical, and pathological study of 79 patients. | to analyze the epidemiology, responsible agents, clinical features, and outcome of white superficial onychomycosis (wso). | 2004 | 15210460 |
| intra- and interlaboratory study of a method for testing the antifungal susceptibilities of dermatophytes. | the national committee for clinical laboratory standards (nccls) m38-a standard for the susceptibility testing of conidium-forming filamentous fungi does not explicitly address the testing of dermatophytes. this multicenter study, involving six laboratories, investigated the mic reproducibility of seven antifungal agents tested against 25 dermatophyte isolates (5 blinded pairs of five dermatophyte species per site for a total of 300 tests), using the method of dermatophyte testing developed at t ... | 2004 | 15243047 |
| cloning and characterization of trichophyton rubrum genes encoding actin, tri r2, and tri r4. | the three structural genes of trichophyton rubrum encoding actin (3,429 bp) and two antigens, tri r2 (2,950 bp) and tri r4 (3,988 bp), were cloned and characterized. they contained six, four, and five exons, respectively. the t. rubrum actin protein sequence revealed extremely high homology to other fungal actins. | 2004 | 15243098 |
| photodynamic treatment of the dermatophyte trichophyton rubrum and its microconidia with porphyrin photosensitizers. | the application of photosensitizers for the treatment of fungal infections is a new and promising development within the field of photodynamic treatment (pdt). dermatophytes, fungi that can cause infections of the skin, hair and nails, are able to feed on keratin. superficial mycoses are probably the most prevalent of infectious diseases in all parts of the world. one of the most important restrictions of the current therapeutic options is the return of the infection and the duration of the trea ... | 2004 | 15244503 |
| scanning electron microscope imaging of onychomycosis. | although scanning electron microscope technology has been used for more than 60 years in many fields of medical research, no studies have focused on obtaining high-resolution microscopic images of onychomycosis of the toenail caused by trichophyton rubrum in a geriatric population. to provide new insight into the intricate structure and behavior of chronic toenail onychomycosis, we produced three-dimensional images of onychomycosis obtained from two geriatric patients with confirmed growth of t ... | 2004 | 15265994 |
| frequency and risk factors of dermatophytosis in students living in rural areas in eskişehir, turkey. | our study included 2384 students from five villages around eskişehir, turkey. we asked every student for their personal identification and also for their sanitation in order to get an idea about dermatophytosis. samples taken from suspicious lesion were collected and inoculated onto sabouraud dextrose agar slants. for identification of fungi which were grown, macroscopic appearance of colonies, microscopic examination and biochemical tests were used. we found suspicious lesions in 245 (10.3%) an ... | 2004 | 15281399 |
| onychomycosis caused by an isolate conforming to the description of trichophyton raubitschekii. | trichophyton raubitschekii is currently regarded as a synonymous name betokening a variant form of trichophyton rubrum. nonetheless, isolates conforming to this morphotaxonomic concept have morphological, physiological and clinical features very different from those of typical t. rubrum. isolates are mainly obtained from subjects originating from certain tropical and subtropical countries, and are mainly obtained from upper body skin infections, rarely from onychomycosis. in this paper the autho ... | 2004 | 15283242 |
| pcr and pcr-rflp techniques targeting the dna topoisomerase ii gene for rapid clinical diagnosis of the etiologic agent of dermatophytosis. | we have focused on the dna topoisomerase ii genes of several pathogenic fungi, and developed polymerase chain reaction (pcr) and pcr-restriction fragment length polymorphism (rflp) methods targeting this gene for identification of dermatophytes. | 2004 | 14757280 |
| antimicrobial activity of hyptis ovalifolia towards dermatophytes. | the essential oil and the aqueous, hexane and methanolic fractions from hyptis ovalifolia leaves were evaluated for their antifungal activity in vitro against 60 strains of dermatophytes: 10 strains of microsporum canis, 10 of m. gypseum, 20 of trichophyton rubrum and 20 of t. mentagrophytes. the extracts inhibited growth of the dermatophytes tested at different concentrations. the most biologically active was the essential oil from the leaves which inhibited 57 isolates (95%) at a concentration ... | 2004 | 14762526 |
| multiplication of an ancestral gene encoding secreted fungalysin preceded species differentiation in the dermatophytes trichophyton and microsporum. | dermatophytes are human and animal pathogenic fungi which cause cutaneous infections and grow exclusively in the stratum corneum, nails and hair. in a culture medium containing soy proteins as sole nitrogen source a substantial proteolytic activity was secreted by trichophyton rubrum, trichophyton mentagrophytes and microsporum canis. this proteolytic activity was 55-75 % inhibited by o-phenanthroline, attesting that metalloproteases were secreted by all three species. using a consensus probe co ... | 2004 | 14766908 |
| derivatives of phosphate schiff base transition metal complexes: synthesis, studies and biological activity. | we report the synthesis and structural characterization of series of tetra- and hexacoordinate metal chelate complexes of phosphate schiff base ligands having the general composition lmx(n).h(2)o and l(2)mx(n) (l=phosphate schiff base ligand; m=ag(+), mn(2+), cu(2+), zn(2+), cd(2+), hg(2+), or fe(3+) and x=no(3)(-), br(-) or cl(-)). the structure of the prepared compounds was investigated using elemental analysis, ir, 1h and 31p nmr, uv-vis, mass spectra, solid reflectance, magnetic susceptibili ... | 2004 | 14670488 |
| antifungal activity of fluid extract and essential oil from anise fruits (pimpinella anisum l., apiaceae). | antifungal activities of fluid extract and essential oil obtained from anise fruits pimpinella anisum l. (apiaceae) were tested in vitro on clinical isolates of seven species of yeasts and four species of dermatophytes. diffusion method with cylinders and the broth dilution method were used for antifungal activity testing. anise fluid extract showed antimycotic activity against candida albicans, c. parapsilosis, c. tropicalis, c. pseudotropicalis and c. krusei with mic values between 17 and 20% ... | 2005 | 16375827 |
| trichophyton rubrum infection of the prepuce. | 2005 | 16394393 | |
| antidermatophytic and bacterial activity of mimosine. | mimosine, a non-protein aromatic amino acid was tested at 100, 50 and 25 microg/ml on some human pathogenic bacteria and fungi. mimosine exhibited total lethality towards trichophyton tonsurans and trichophyton rubrum at 100 microg/ml. among the tested bacteria staphylococcus aureus was inhibited to a larger extent than the other bacteria. the studies revealed mimosine to be potent against fungi rather than bacteria. this study reports the effect of mimosine on dermatophytes for the first time. | 2005 | 16317659 |
| [onychomycosis in morocco: experience of the parasitology and medical mycology laboratory from rabat children hospital (1982-2003)]. | cases of onychomycosis diagnosed by mycological examination in the parasitology and mycology laboratory (children hospital, rabat, marocco) over the 22-year period, between 1982 and 2003, have been reviewed. 17,177 dermatophytes have been isolated in 4,940 patients. dermatophytes presented 61.46% of onychomycosis, yeasts candida albicans were responsible in 25.5%, moulds in 1.53% and for 12% fungi identification was not determined (positive direct examination or negative direct examination and c ... | 2005 | 16330383 |
| epidemiology of tinea pedis and toenail tinea unguium in worshippers in the mosques in adana, turkey. | the aim of this study was to determine the prevalence of dermatomycosis and the risk factors in those who perform their prayers in the mosques in the city center of adana province. the diagnosis of dermatomycosis was made on the basis of direct microscopy and/or culture in addition to clinical findings. among 461 subjects, 136 (29.5%) had tinea pedis, 23 (5.0%) had tinea unguium, and 21 (4.5%) had both infections simultaneously, with a resulting total of 180 (39.0%) cases of dermatomycosis. the ... | 2005 | 16361711 |
| dermatophyte growth and degradation of human stratum corneum in vitro (pathogenesis of dermatophytosis). | this study was carried out to determine growth of dermatophytes using human stratum corneum in vitro and the degrading effect of keratinases (proteinases) on stratum corneum for a complete understanding of the host parasite relationship. | 2005 | 16599028 |
| analysis of dermatomycoses in lanzhou district of northwestern china. | the skin mycoses, perticularly dermatophytoses, in lanzhou district, northwestern china, was investigated during july 2002-june 2003. the specimens from patients suspected of having dermatomycoses were examined microscopically in koh preparations and cultured on sabouraud dextrose agar (sda). among 1443 suspected cases, 594 were koh positive and 221 cultures of fungi were isolated. the most frequently isolated fungi were trichophyton rubrum (43.9%) trichophyton mentagrophytes (29.4%) and candida ... | 2005 | 16244895 |
| [analysis of secreted proteases of trichophyton rubrum]. | secreted proteases are thought as potential virulent factors of tricophyton rubrum. based on cdna libraries of 6 physiological phases of tricophyton rubrum, 9683 unique ests were obtained by dna sequencing. by bioinformatic analysis, 18 ests of putative secreted proteases were obtained from the established ests library, including 4 secreted peptidases, 1 secreted metalloprotease, 2 extracellular serine proteases, 1 secreted aspartic proteinase, 9 secreted subtilisin-like proteases and 1 vacuolar ... | 2005 | 16245880 |
| markers for host-induced gene expression in trichophyton dermatophytosis. | dermatophytes are adapted to infect keratinized tissues by their ability to utilize keratin as a nutrient source. although there have been numerous reports that dermatophytes like trichophyton sp. secrete proteolytic enzymes, virtually nothing is known about the patterns of gene expression in the host or even when the organisms are cultured on protein substrates in the absence of a host. we characterized the expression of an aminopeptidase gene, the trichophyton mentagrophytes homolog of the tri ... | 2005 | 16177334 |
| onychomycosis in adana, turkey: a 5-year study. | onychomycosis results from invasion of the nail plate by dermatophytes, yeasts or mould species of fungi. this report documents the etiological agents of onychomycosis in adana, turkey. | 2005 | 16207188 |
| onychomycosis in children: a survey of 46 cases. | this is a retrospective study of the agents, clinical aspects, sources of infection and therapy of onychomycosis in children. in the period 1989-2000, we observed 46 consecutive children, until 16 years of age with onychomycosis (29 boys, 17 girls, mean age 10.8 years). dermatophytes were isolated in 30 cases (trichophyton rubrum in 22 cases, trichophyton mentagrophytes in five, epidermophyton floccosum in two and trichophyton violaceum in one) and candida spp. in 16, associated with trichophyto ... | 2005 | 16262881 |
| role of chrysosporium keratinophillum in the parasitic evolution of dermatophytes. | anti-dermatophytic activity of chrysosporium keratinophillum against species of the genera trichophyton, microsporum and epidermophyton floccosum was tested in vitro. when c. keratinophillum and different species of dermatophytes were inoculated on sabouraud's dextrose agar plates 2 cm apart, no antagonistic effect of c. keratinophillum on the mycelial growth of dermatophytes was observed. however, conidia production was not observed on the hyphae of trichophyton rubrum, trichophyton tonsurans a ... | 2005 | 16262884 |
| trichophyton rubrum skin infection in two premature infants. | trichophyton rubrum is a dermatophyte that causes tinea infection in adults and children but infection is surprisingly rare in the preterm infant. we present two cases of t. rubrum infection in preterm infants, in which mother-to-child transmission appears to have occurred. we describe the clinical features, differential diagnosis, appropriate investigation and management of this condition. | 2005 | 16014146 |
| single strains of trichophyton rubrum in cases of tinea pedis. | multiple colonies of trichophyton rubrum were isolated from single skin specimens from 10 patients with tinea pedis and were typed using a pcr-based analysis of repeats in the rrna intergenic spacer. in each case only a single strain type of t. rubrum was isolated, suggesting a monotypic aetiology of tinea pedis. this is in contrast to the multiple strains previously shown to be involved in many cases of onychomycosis. | 2005 | 16014424 |
| onychomycosis in ceará (northeast brazil): epidemiological and laboratory aspects. | knowledge of epidemiological and mycological characteristics of onychomycosis has been noted by many authors as being an important tool for control of these fungal infections. this study seeks to improve knowledge of onychomycosis epidemiology and mycological features. samples were taken from infected fingernails and toenails of 976 patients undergoing treatment at a respected dermatology center in ceará, fortaleza, ce, brazil. specimens from 512 patients (52%) were positive for onychomycosis. f ... | 2005 | 16021299 |
| first report on autochthonous urease-positive trichophyton rubrum (t. raubitschekii) from south-east europe. | trichophyton raubitschekii is a dermatophyte belonging to the t. rubrum complex and is differentiated principally by its positive urease activity and production of profuse macroconidia and microconidia in culture. it is classically isolated from african, south-east asian and australian aboriginal patients with tinea corporis or tinea cruris. | 2005 | 16029346 |
| antifungal susceptibilities of dermatophytic agents isolated from clinical specimens. | the aim of this study was to investigate the susceptibility to four antifungal agents: ketoconazole, terbinafine, itraconazole and fluconazole, of the different species of dermatophyte strains isolated from clinical specimens. a total of 128 specimens were collected from toe nail, foot, inguinal region, trunk, hands and head. the dermatophytes tested included trichophyton rubrum 108 (84.4%), trichophyton mentagrophytes 11 (8.6%), epidermophyton floccosum 5 (3.9%), microsporum canis 2 (1.5%) and ... | 2005 | 16048753 |
| in vitro antifungal assay of traditional argentine medicinal plants. | methanol extracts from 11 traditionally used argentine medicinal plants were assayed in vitro for antifungal activity against yeasts, hialohyphomycetes as well as dermatophytes with the microbroth dilution method. among them, the strongest effect was presented by eupatorium buniifolium and terminalia triflora, trichophyton mentagrophytes and trichophyton rubrum being the most susceptible species with mics ranging from 100 to 250 microg/ml. lithrea molleoides showed the broadest spectrum of actio ... | 2005 | 16055292 |
| dermatophytes growth curve and in vitro susceptibility test: a broth micro-titration method. | the introduction of systemic antifungal drugs which act upon different targets is the main issue of the in vivo antifungal resistance control. different factors, such as growth curve phase, quality of the specimen, quantity of the inoculum, temperature, ph, culture medium composition, incubation duration and solvent, are believed important factors affecting minimum inhibitory concentration (mic) value to most of the antifungal agents. we assayed an in vitro susceptibility test with 40 isolates o ... | 2005 | 16110777 |
| eighty-four consecutive cases of tinea faciei in siena, a retrospective study (1989-2003). | consecutive cases of tinea faciei diagnosed in siena between 1989 and 2003 were studied retrospectively for differences in clinical form, demographic data and species of dermatophyte isolated. the series consisted of 84 cases (59 females, 25 males) with a mean age of 27 years. mean age of females (32.4 years) was significantly greater than that of males (14.2 years). the dermatophytes most frequently isolated were microsporum canis (38 cases) and trichophyton rubrum (31 cases). clinical form was ... | 2005 | 16115106 |
| the importance of mycological investigations in diagnostics of nail changes. | the aim of the study was an analysis of correlation between mycological examination results and clinical features of changed toenails in patients that visited mycological laboratory due to suspected onychomycosis. | 2005 | 16119622 |
| patients at risk of onychomycosis--risk factor identification and active prevention. | objectives: the aims of this workshop were to identify risk factors for onychomycosis and to reach consensus on the management of high-risk groups to allow the development of guidelines to help doctors recognize risk factors that complicate treatment. results and conclusions: previous trichophyton rubrum infection, older age, abnormal nail morphology, immunodeficiency and genetic factors were identified as risk factors for initial infections. risk factors for recurrence (relapse and re-infection ... | 2005 | 16120200 |
| genetic predisposition--understanding underlying mechanisms of onychomycosis. | there are numerous factors that predispose to onychomycosis including genetic predisposition, diabetes mellitus, immunosuppression, vascular disease and psoriasis. | 2005 | 16120201 |
| [enzyme activities of dermatophytes isolated from different clinical samples by apizym method]. | dermatophytes' enzymes may have a role in chronic superficial infections. in this study it was planned to investigate the possible relationship between the enzymatic activities of the dermatophytes and acute or chronic course of the infections. dermatophytes were isolated from 58 (72%) out of 81 patients with superficial infections. the infections were divided into two groups as acute (< or = 6 weeks) or chronic (> 6 weeks). isolated fungi were identified by the classical methods and apizym meth ... | 2005 | 16128029 |
| in vitro antimycotic activity and nail permeation models of a piroctone olamine (octopirox) containing transungual water soluble technology. | several in vitro studies with a new medical device (myfungar) containing 0.5% of piroctone olamine (cas 68890-66-4, octopirox) in a hydroxypropyl chitosan hydroalcoholic solution were performed. the chemical name of the active ingredient is 1-hydroxy-4-methyl-6 (2,4,4-trimethylpentyl)-2(1h)-pyridone; combination with 2-amino-ethanol (1:1). the antimycotic activity was determined in the most common fungi responsible of nail infections such as candida parapsilosis, scopulariopsis brevicaulis or tr ... | 2005 | 16149717 |
| trichophyton rubrum isolated from aids and human immunodeficiency virus-infected patients in são paulo, brazil: antifungal susceptibility and extracellular enzyme production. | in order to identify intraspecific variations in trichophyton rubrum and to correlate them to the immunological status of the host, sixty strains isolated from aids, hiv-positive and hiv-negative patients were compared for the production of extracellular enzymes and for their susceptibility to several antifungal drugs. | 2005 | 15722629 |
| antifungal effect of 5-aminolevulinic acid pdt in trichophyton rubrum. | in the present investigation, we have shown for the first time that the onychomycosis-inducing dermatophyte trichophyton rubrum was able to metabolize 5-aminolevulinic acid (ala) to protoporphyrin ix (ppix) in liquid culture medium. we have established and optimized the culture conditions and could show the typical ppix-induced red fluorescence which was evaluated qualitatively by wood's light examination and fluorescent microscopic analysis. the optimum concentration of ala was in the range of ... | 2005 | 15743426 |
| proteolytic peptide patterns as indicators for fungal infections and nonfungal affections of human nails measured by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. | the discrimination of onychomycoses from endogenous diseases showing macroscopically similar symptoms is difficult. long-lasting but ineffective antifungal therapies using systemic medicaments with often severe adverse reactions may be the consequence. we introduce a novel mass spectrometric method for the discrimination of fungal infections and nonfungal affections. horn samples from patients infected by trichophyton rubrum, from patients with psoriasis affecting nails, and from healthy persons ... | 2005 | 15745754 |
| [superficial mycoses: comparative study between type 2 diabetic patients and a non-diabetic control group]. | superficial mycoses are considered to affect more frequently patients with type 2 diabetes mellitus (dm-2), specially onychomycosis and tinea pedis. the purpose of this study was to compare the dermatophytoses, candidiasis and pitiriasis versicolor frequency between 40 patients with dm-2 and 40 healthy persons of either sex, 40 years old or more. clinical, metabolic, mycologic and inmunologic studies against candida albicans, were carried out. both diabetics 75% (30/40) and controls 65% (26/40) ... | 2005 | 15782538 |
| antifungal activity and studies on mode of action of novel xanthoxyline-derived chalcones. | chalcones and chalcone-like compounds, most of them new ones, prepared by base-catalyzed condensation of appropriate aldehydes and xanthoxyline, were tested for antifungal properties against a panel of yeasts, hialohyphomycetes as well as dermatophytes with the agar dilution assay. results indicate that neither the sole presence of a "xanthoxyline-like" substitution pattern nor a 2'-oh substituent on ring a are sufficient for these compounds to have antifungal properties. the chalcone 3-(2-chlor ... | 2005 | 15799012 |
| fungal infection of the feet in soccer players and non-athlete individuals. | this study was conducted to evaluate the occurrence of mycoses affecting the feet of soccer players and to compare this results with those in non-athlete individuals of the same age and sex. initial evaluation consisted of a dermatological examination of the foot in 22 chinese athletes, 83 brazilian athletes and 24 brazilian non-athletes. scales of plantar skin, interdigital and subungual areas of the foot were collected for mycological examination (direct and culture). nail clippings were obtai ... | 2005 | 15813681 |
| evaluation of broth microdilution antifungal susceptibility testing conditions for trichophyton rubrum. | fifty clinical isolates of trichophyton rubrum were selected to test with ketoconazole, fluconazole, itraconazole, griseofulvin, and terbinafine by following the national committee for clinical laboratory standards susceptibility testing guidelines for filamentous fungi (m38-a). in addition, other susceptibility testing conditions were evaluated: (i) three medium formulations including rpmi 1640 (standard medium), mcveigh & morton (mvm), and sabouraud dextrose broth (sdb); (ii) two incubation te ... | 2005 | 15815018 |
| [dermatophytosis with concurrent lesions in distant locations. prognostic and therapeutic significance]. | recurrence and a lack of treatment response are common in dermatophytosis. in patients with cutaneous and concurrent toenail lesions, often only the former are investigated, which may result in inappropriate treatment due to misdiagnosis. | 2005 | 15826541 |
| antifungal activity of ocimum gratissimum towards dermatophytes. | the development of more effective and less toxic antifungal agents is required for the treatment of dermatophytosis. plants and their preparations have been used as medicines against infectious diseases. extracts of ocimum gratissimum leaves were investigated for in vitro antifungal activity, using agar dilution technique against dermatophytes. the extracts (hexane, chloroform fractions, the essential oil and eugenol) produced antifungal activities against microsporum canis, m. gypseum, trichoph ... | 2005 | 15842332 |
| dermatophyte infections in the ljubljana region, slovenia, 1995-2002. | we studied dermatophyte infections in patients, examined in the mycological laboratory of the department of dermatology, university medical centre ljubljana during the period 1995-2002. a total of 42,494 samples were collected from 33,974 patients suspected to have dermatomycosis. dermatophytes were identified in 8286 (71.2%) positive cultures. microsporum canis was the most frequent dermatophyte isolated (46.8%), followed by trichophyton rubrum (36.7%), t. mentagrophytes var. interdigitale (7.9 ... | 2005 | 15842334 |
| nine male cases of tinea genitalis. | nine cases of tinea genitalis observed in siena and terni (italy) between 1988 and 2003 are reported because of their infrequency. the patients were males ranging in age from 23 to 45 years. the lesions, situated on the penis, glans and scrotum, were preceded by dermatophytosis in other sites (groin five cases; feet two cases; toenails two cases; hands in one; beard in another). mycological examination consisting of direct microscopy and culture led to isolation of trichophyton rubrum in five ca ... | 2005 | 15842338 |
| comparison of in vitro activities of antifungal drugs and ethanolic extract of propolis against trichophyton rubrum and t. mentagrophytes by using a microdilution assay. | the in vitro activities of propolis against 29 strains of dermatophytes were compared with those of terbinafine, itraconazole, ketoconazole, and fluconazole. minimal inhibitory concentrations (mics) were determined according to a national committee for clinical laboratory standards broth microdilution method. among the systemic antifungals tested, terbinafine was the most potent. propolis showed important antifungal activity and it merits further investigation as a potentially useful agent for t ... | 2005 | 15842339 |
| superficial fungal infections seen at the national skin centre, singapore. | cutaneous fungal infections are common in singapore. the national skin centre is a tertiary referral centre for dermatological diseases in the country, and sees more than 2,500 cases of superficial fungal infections annually. | 2005 | 15864251 |
| identification of trichophyton rubrum by nested pcr analysis from paraffin embedded specimen in trichophytia profunda acuta of the glabrous skin. | trichophytia profunda acuta of the glabrous skin (tpags) arose in a 67-year-old japanese man. the patient presented indurated erythematous plaques and nodules on his left forearm. direct microscopic examination of the scale in koh preparation was negative for fungal elements, and culture for dermatophytes was also negative. although fungal infection could not be proven in hematoxillin-eosin stained sections, deep-cut sections of the biopsied skin lesion with pas stain revealed the ectothrix pres ... | 2005 | 15864260 |
| repetitive-sequence-pcr-based dna fingerprinting using the diversilab system for identification of commonly encountered dermatophytes. | the performance of repetitive-sequence-based pcr (rep-pcr) using the diversilab system for identification of dermatophytes commonly isolated in a clinical laboratory was assessed by comparing results to those of conventional tests (colony morphology, microscopic examination of slide cultures, and, for suspected trichophyton species, use of additional media). sixty-one cultures were tested in phase 1, the feasibility portion of the study; 64 additional cultures were tested in phase 2, the validat ... | 2005 | 15872233 |
| a prospective epidemiologic survey on the prevalence of onychomycosis and dermatophytosis in male boarding school residents. | dermatophyte infections and onychomycosis are not usually serious in term of mortality; however, they may have significant clinical consequences such as secondary bacterial infections, chronicity, therapeutic difficulties and esthetic disfigurement in addition to serving as a reservoir of infection. our aim was to determine the prevalence of onychomycosis and dermatophytosis in a selected high risk group, consisting of male boarding school residents. a total of 410 males inhabiting two houses we ... | 2005 | 15883717 |
| tinea cruris in routine urology practice. | tinea cruris is almost exclusively a male dermatophytosis. this infection is also sexually transmitted, and may cause epidemics in public areas such as common bathing facilities, dormitories and among military recruits. it has been aimed to investigate the prevalence and the causative agents of tinea cruris and tinea pedis in patients who were admitted to the department of urology with various pathologies. | 2005 | 15897702 |
| antidermatophyte activity of ether extract of nigella sativa and its active principle, thymoquinone. | the antifungal activity of ether extract of nigella sativa seed and its active principle thymoquinone was tested against eight species of dermatophytes: four species of trichophyton rubrum and one each of trichophyton interdigitale, trichophyton mentagrophytes, epidermophyton floccosum and microsporum canis. agar diffusion method with serial dilutions of ether extract of nigella sativa, thymoquinone and griseofulvin was employed. the incubation was carried out at 30 degrees c for 14 days. the di ... | 2005 | 15908151 |
| [evaluation the antimycotic activity of itraconazole in human stratum corneum with vitro fungi cultivation]. | to establish a new model to evaluate the antifungal activity of antimycotics. | 2005 | 15949332 |
| amino acid substitution in trichophyton rubrum squalene epoxidase associated with resistance to terbinafine. | there has only been one clinically confirmed case of terbinafine resistance in dermatophytes, where six sequential trichophyton rubrum isolates from the same patient were found to be resistant to terbinafine and cross-resistant to other squalene epoxidase (se) inhibitors. microsomal se activity from these resistant isolates was insensitive to terbinafine, suggesting a target-based mechanism of resistance (b. favre, m. ghannoum, and n. s. ryder, med. mycol. 42:525-529, 2004). in this study, we ha ... | 2005 | 15980358 |
| dermatophytoses in forestry workers and farmers. | to assess the frequency of superficial mycoses in forestry workers and farmers in the rural region of duzce, a total of 467 residents of the rural region were examined for dermatomycosis infection. of these, 349 were forestry workers and 118 farmers. all specimens collected were analysed by direct microscopy and culture. tinea pedis et manus was found in 23 (19.4%), and onycomycosis in 21 (17.7%) farmers. tinea pedis et manus was found in 50 (14.3%), and onycomycosis in 28 (8%) forestry workers. ... | 2005 | 15982208 |
| [the raubitschekii variant of trichophyton rubrum has reached germany]. | this is the second report on an isolation of the variety raubitschekii of trichophyton (t.) rubrum in germany. the patient was a young man born in cameroon who presented with an extensive tinea corporis et manuum. the variety raubitschekii can be distinguished from similar looking dermatophytes by its brownish color, by the formation of abundant t. rubrum-like macroconidia, by the production of urease, by a negative hair perforation test, and by dna analysis. in addition, further characteristics ... | 2005 | 15619081 |
| prevalence of tinea capitis and tinea pedis in barcelona schoolchildren. | although dermatophytoses can appear at any age, some types are particularly prevalent in children. there are no prior data on the prevalence of tinea capitis and tinea pedis in barcelona, spain. to identify the prevalence of tinea in school children in the area with the highest immigrant population in this city, a cross-sectional study was performed. a second objective was to identify the etiologic agent to study the possibility of the introduction of foreign dermatophyte species and to evaluate ... | 2005 | 15702042 |
| in vitro antifungal activity of dragon's blood from croton urucurana against dermatophytes. | based on ethnobotanical approach, the dragon's blood collected from croton urucurana baill. bark (euphorbiaceae) was tested for antifungal activity against five dermatophytes by paper disk diffusion method. the minimal inhibitory concentration (mic) showing no visible fungal growth was also determined, using tube dilution technique. the test dermatophytes were tricophyton tonsurans, trichophyton mentagrophytes, trichophyton rubrum, microsporum canis and epidermophyton floccossum. the dragon's bl ... | 2005 | 15707783 |
| use of magnetic beads to extract fungal dna. | authors compare two methods of extracting dna from different fungi: the classic method with phenol/chloroform (p/c) and that with magnetic beads. both were tested on candida albicans and cryptococcus neoformans var. neoformans, belonging to the yeast group and microsporum canis, m. gypseum, trichophyton rubrum, t. interdigitale, t. ajelloi, epidermophyton floccosum, belonging to the dermatophytes group. extraction products underwent polymerase chain reaction (pcr) fingerprinting with the appropr ... | 2005 | 15679657 |
| epidemiology of dermatomycoses in children living in northern greece 1996-2000. | in the 5-years period, 1996-2000, 1045 children under 13 years old were examined for suspected dermatomycosis. in 611 cases fungi were isolated. male children were mainly affected on the scalp and body area. girls were more affected in the location of the arms and legs. there was a greater proportion of cases in the age range 2-12 years. the most prominent fungus was microsporum canis (515 cases) followed by trichophyton rubrum (34) and candida albicans (20). tinea capitis (280 cases) mainly cau ... | 2005 | 15679659 |
| retrospective study of onychomycosis in italy: 1985-2000. | cases of onychomycosis diagnosed by mycological examination in three mycology units (florence, siena and milan) of central and northern italy over the 15-year period, 1985-2000, were studied retrospectively. the number of cases was 4046 (1952 women, 2094 men). dermatophytes were isolated in 2859, yeasts in 655 and moulds in 532 cases. the most frequent dermatophyte was trichophyton rubrum (87%), followed by t. mentagrophytes var. interdigitale (10%). candida albicans (93.2%) was the prevalent ye ... | 2005 | 15679665 |
| trichophyton rubrum-induced inflammatory tinea capitis in a 63-year-old man. | tinea capitis caused by trichophyton rubrum is a rare event worldwide. we report on an elderly otherwise healthy man with inflammatory fungal disease of the scalp caused by this pathogen. he had no signs of concomitant mycosis of the skin and nails and no underlying immunosuppressive disease. the fungal infection primarily had been misdiagnosed in terms of a pyoderma. failure of antibiotic treatment, a positive mycological study and molecular diagnostics eventually led to the correct diagnosis. ... | 2005 | 15679672 |
| aminopeptidases and dipeptidyl-peptidases secreted by the dermatophyte trichophyton rubrum. | the nature of secreted aminopeptidases in trichophyton rubrum was investigated by using a reverse genetic approach. t. rubrum genomic and cdna libraries were screened with aspergillus spp. and saccharomyces cerevisiae aminopeptidase genes as the probes. two leucine aminopeptidases, rulap1 and rulap2, and two dipeptidyl-peptidases, rudppiv and rudppv, were characterized and compared to orthologues secreted by aspergillus fumigatus using a recombinant protein from pichia pastoris. rulap1 is a 33 k ... | 2005 | 15632434 |
| bilateral breast enlargement due to trichophyton rubrum. | gynecomastia is the most common cause of breast enlargement in males. trichophyton rubrum is a common dermatophyte, is responsible for a variety of infections, and may rarely manifest as a dermatophytic pseudomycetoma. we report the case of a 52-year-old man who presented with progressive bilateral breast enlargement. this is the first reported case of bilateral breast pseudomycetoma due to t. rubrum. long-term antifungal therapy with itraconazole was successful. | 2006 | 15998588 |
| chemical composition and antidermatophytic properties of volatile fractions of hexanic extract from leaves of cupressus lusitanica mill. from cameroon. | the chemical composition of five column fractions of hexanic leaf extract of cupressus lusitanica were analysed by gas chromatography and gas chromatography-mass spectrometry and then tested for their antidermatophytic activities using the agar dilution method. the first fraction (f(1)) has only hydrocabon monoterpenes with alpha-pinene (80.0%) as major component. the main constituents of the second fraction (f(2)) were epi-bicyclosesquiphellandrene (35.3%), epi-zonarene (10.3%), 1s, cis-calamen ... | 2006 | 16169171 |
| activity of the triazole antifungal r126638 as assessed by corneofungimetry. | r126638 is a novel triazole exhibiting potent in vitro and in vivo antifungal activity against fungal pathogens including dermatophytes and yeasts. | 2006 | 16247249 |
| antimicrobial, wound healing and antioxidant activity of plagiochasma appendiculatum lehm. et lind. | plagiochasma appendiculatum (aytoniaceae) of the order marchantiales is widely used in the form of paste ethnomedicinally by gaddi tribe in kangra valley for treating skin diseases. in this context, antimicrobical potential of plagiochasma appendiculatum against a wide range of microorganisms was studied. to validate the ethnotherapeutic claims of the plant in skin diseases, wound healing activity was studied, besides antioxidant activity to understand the mechanism of wound healing activity. th ... | 2006 | 16600543 |
| epidemiology of superficial mycosis (tinea pedis, onychomycosis) in elementary school children in istanbul, turkey. | the purpose of this study is to determine the prevalence of tinea pedis and onychomycosis in children of elementary school age and to examine the socio-demographic attributes that may be effective in correlation of both mycoses. 3,390 female and 3,768 male children between ages 6-14 have been examined in seven schools. skin scrapings and nail samples were taken from 13 students who were suspected to have tinea pedis and from 49 students who were suspected to have onychomycosis. according to dire ... | 2006 | 16617585 |
| synthesis and evaluation of antimicrobial and anticonvulsant activities of some new 3-[2- (5-aryl-1,3,4-oxadiazol-2-yl/4-carbethoxymethylthiazol-2-yl) imino-4-thiazolidinon-5-ylidene]-5-substituted/nonsubstituted 1h-indole-2-ones and investigation of their structure-activity relationships. | in the present study, 20 new compounds having 3-[2-(5-aryl-1,3,4-oxadiazol-2-yl) imino-4-thiazolidinon-5-ylidene]-5-substituted/nonsubstituted 1h-indole-2-one (i-xii) and 3-[2-(4-carbethoxymethylthiazol-2-yl)imino-4-thiazoldinon-5-ylidenel-5-substituted/nonsubstituted ih-indole-2-one (xiii-xx) systems were synthesized. the structures were confirmed by spectral methods (uv, ir, 1h-nmr, 13c-nmr, 13c-dept (135), electron impact mass spectrometry) and elemental analysis. all compounds were tested fo ... | 2006 | 16618017 |
| [a new bioassay model of antibiotic susceptibility by corneofungimetry]. | to study the value of a new bioassay model of antibiotic susceptibility by corneofungimetry which is more similar to human internal environment. | 2006 | 16677528 |