Publications

investigation of cr(vi) adsorption onto chemically treated helianthus annuus: optimization using response surface methodology.	in the present study, chemically treated helianthus annuus flowers (shc) were used to optimize the removal efficiency for cr(vi) by applying response surface methodological approach. the surface structure of shc was analyzed by scanning electron microscopy (sem) coupled with energy dispersive x-ray analysis (edx). batch mode experiments were also carried out to assess the adsorption equilibrium in aqueous solution. the adsorption capacity (qe) was found to be 7.2 mg/g. the effect of three parame ...	2011	20739179
localization of lipoxygenase activity on the oil bodies and in protoplasts using a novel fluorescence imaging method.	lipoxygenase (linoleate:oxygen oxidoreductase; ec 1.13.11.12; lox) catalyzes oxygenation of polyenoic fatty acids, which precedes the degradation of storage lipids during seed germination in sunflower. in the present work, it has been confirmed that 2',7'-dichlorodihydrofluorescein diacetate (h(2)dcfda) produces fluorescence in presence of lipid hydroperoxides (lox reaction products). this work provides new information on spatial localization of transiently enhanced lox activity in protoplasts f ...	2011	21169027
a transposon-mediate inactivation of a cycloidea-like gene originates polysymmetric and androgynous ray flowers in helianthus annuus.	in several eudicots, including members of the asteraceae family, the cycloidea (cyc) genes, which belong to the tcp class of transcription factors, are key players for floral symmetry. the sunflower inflorescence is heterogamous (radiate capitulum) with sterile monosymmetric ray flowers located in the outermost whorl of the inflorescence and hermaphrodite polysymmetric disk flowers. in inflorescence of heliantheae tribe, flower primordia development initiates from the marginal ray flowers while ...	2011	22552535
genetic diversity and population structure in cultivated sunflower and a comparison to its wild progenitor, helianthus annuus l.	crop germplasm collections are valuable resources for ongoing plant breeding efforts. to fully utilize such collections, however, researchers need detailed information about the amount and distribution of genetic diversity present within collections. here, we report the results of a population genetic analysis of the primary gene pool of sunflower (helianthus annuus l.) based on a broad sampling of 433 cultivated accessions from north america and europe, as well as a range-wide collection of 24 ...	2011	21638000
molecular cloning and characterization of gene coding for γ-tocopherol methyltransferase from lettuce (lactuca sativa).	γ-tocopherol methyltransferase is an important rate-limiting enzyme involved in tocopherol biosynthesis. the full-length cdna encoding γ-tocopherol methyltransferase (designated as lstmt) was cloned from lactuca sativa for the first time by rapid amplification of cdna ends and characterized by means of quantitative rt-pcr. the full-length cdna of lstmt was 1131 bp, with an open reading frame of 897 bp encoding a γ-tocopherol methyltransferase protein of 298 amino acids, with a calculated molecul ...	2011	22194177
sunflower stem weevil and its larval parasitoids in native sunflowers: is parasitoid abundance and diversity greater in the u.s. southwest?	classical biological control programs often target a pest's region of origin as a likely source for new biological control agents. here, we use this approach to search for biological control agents of the sunflower stem weevil (cylindrocopturus adspersus leconte), an economically important pest of commercial sunflower. we conducted surveys of weevil natural enemy diversity and abundance across a transect running from the northern great plains to the southwestern u.s. (the presumed area of endemi ...	2011	22182606
characterization of long transcribed microsatellites in helianthus annuus (asteraceae).	research on the evolutionary role of exonic microsatellites currently lacks an understanding of the evolutionary pressures that promote or limit their expansion. contrasting levels of variability and genetic structures at anonymous and transcribed microsatellite loci of varying lengths are likely to provide useful insights regarding the relative strength of selection acting on different classes of microsatellites. we have developed primers for long transcribed microsatellites in helianthus annuu ...	2011	22130271
copper phytoextraction in tandem with oilseed production using commercial cultivars and mutant lines of sunflower.	use of sunflower (helianthus annuus l.) for cu phytoextraction and oilseed production on cu-contaminated topsoils was investigated in afield trial at a former wood preservation site. six commercial cultivars and two mutant lines were cultivated in plots with and without the addition of compost (5% w/w) and dolomitic limestone (0.2% w/w). total soil cu ranged from 163 to 1170 mg kg(-1). in soil solutions, cu concentration varied between 0.16-0.93 mg l(-1). the amendment increased soil ph, reduced ...	2011	22046751
greenhouse gas emissions and plant characteristics from soil cultivated with sunflower (helianthus annuus l.) and amended with organic or inorganic fertilizers.	agricultural application of wastewater sludge has become the most widespread method of disposal, but the environmental effects on soil, air, and crops must be considered. the effect of wastewater sludge or urea on sunflower's (helianthus annuus l.) growth and yield, the soil properties, and the resulting co(2) and n(2)o emissions are still unknown. the objectives of this study were to investigate: i) the effect on soil properties of organic or inorganic fertilizer added to agricultural soil cult ...	2011	22033361
increased growth in sunflower correlates with reduced defences and altered gene expression in response to biotic and abiotic stress.	cultivated plants have been selected by humans for increased yield in a relatively benign environment, where nutrient and water resources are often supplemented, and biotic enemy loads are kept artificially low. agricultural weeds have adapted to this same benign environment as crops and often have high growth and reproductive rates, even though they have not been specifically selected for yield. considering the competing demands for resources in any plant, a key question is whether adaptation t ...	2011	21988641
pollen aroma fingerprint of two sunflower (helianthus annuus l.) genotypes characterized by different pollen colors.	samples of fresh pollen grains, collected from capitula in full bloom from two genotypes of sunflower (helianthus annuus l.) and characterized by a different color, i.e., white-cream (wc) and orange (o), were analyzed by the hs-spme (headspacesolid phase microextraction)/gc/ms technique. this study defined for the first time the fingerprint of the sunflower pollen, separated from the disc flowers, to define its contribution to the inflorescence aroma. in the gc/ms fingerprints of the wc and o g ...	2011	21922665
shedding light on the evolution of plasticity in natural populations.	plasticity allows for changes in phenotype in response to environmental cues, often facilitating local adaptation to seasonal environments. phenotypic plasticity alone, however, may not always be sufficient to ensure adaptation to new localities. in particular, changing cues associated with shifting seasonal regimes may no longer induce appropriate phenotypic responses in new environments (nicotra et al. 2010). plastic responses must thus evolve to avoid being maladaptive. to date, the extent to ...	2011	21884290
impact of planting dates on a seed maggot, neotephritis finalis (diptera: tephritidae), and sunflower bud moth (lepidoptera: tortricidae) damage in cultivated sunflower.	neotephritisfinalis (loew) (diptera: tephritidae), and sunflower bud moth, suleima helianthana (riley) (lepidoptera: tortricidae) are major head-infesting insect pests of cultivated sunflower (helianthus annuus l.). planting date was evaluated as a cultural pest management strategy for control of n. finalis and s. helianthana in several production regions of north dakota during 2009 and 2010. results of the nine site-year study revealed that late planting date (early to mid-june) reduced damage ...	2011	21882688
dna alteration and programmed cell death during ageing of sunflower seed.	sunflower (helianthus annuus l.) seed viability is affected by moisture content (mc) during ageing and is related to accumulation of hydrogen peroxide and changes in energy metabolism. the aim of the present work was to investigate the effect of ageing on dna alteration events by rapd (random amplification of polymorphic dna) analysis and to determine whether loss of seed viability might correspond to a controlled programmed cell death (pcd). ageing of sunflower seeds was carried out at 35 °c fo ...	2011	21765164
catalase is a key enzyme in seed recovery from ageing during priming.	ageing induces seed deterioration expressed as the loss of seed vigour and/or viability. priming treatment, which consists in soaking of seeds in a solution of low water potential, has been shown to reinvigorate aged seeds. we investigate the importance of catalase in oxidation protection during accelerated ageing and repair during subsequent priming treatment of sunflower (helianthus annuus l.) seeds. seeds equilibrated to 0.29g h2og(-1) dry matter (dm) were aged at 35°c for different durations ...	2011	21763542
connecting the sun to flowering in sunflower adaptation.	species living in seasonal environments often adaptively time their reproduction in response to photoperiod cues. we characterized the expression of genes in the flowering-time regulatory network across wild populations of the common sunflower, helianthus annuus, that we found to be adaptively differentiated for photoperiod response. the observed clinal variation was associated with changes at multiple hierarchical levels in multiple pathways. paralogue-specific changes in ft homologue expressio ...	2011	21676045
high temperature triggers the metabolism of s-nitrosothiols in sunflower mediating a process of nitrosative stress which provokes the inhibition of ferredoxin-nadp reductase by tyrosine nitration.	high temperature (ht) is considered a major abiotic stress that negatively affects both vegetative and reproductive growth. whereas the metabolism of reactive oxygen species (ros) is well established under ht, less is known about the metabolism of reactive nitrogen species (rns). in sunflower (helianthus annuus l.) seedlings exposed to ht, no content as well as s-nitrosoglutathione reductase (gsnor) activity and expression were down-regulated with the simultaneous accumulation of total s-nitroso ...	2011	21676000
physiology and proteomics of drought stress acclimation in sunflower (helianthus annuus l.).	an easy and manageable in vitro screening system for drought tolerance of sunflower seedlings based on ms media supplemented with polyethylene glycol 6000 was evaluated. morphological and physiological parameters were compared between control (-0.05 mpa) and drought-stressed (-0.6 mpa) seedlings of helianthus annuus l. cv. peredovick. there was a significant growth deficit in drought-stressed plants compared to control plants in terms of hypocotyl length, and shoot and root fresh mass. shoot gro ...	2011	21668604
detection of single copy sequences using bac-fish and c-prins techniques in sunflower chromosomes.	bacterial artificial chromosome-fluorescence in situ hybridization (bac-fish) and cycling-primed in situ labeling (c-prins) techniques were evaluated for integration of physical and genetic maps of sunflower (helianthus annuus l.). single-site ssr markers were selected from three linkage groups of a high-density sunflower genetic map. this selection was based on previously identified qtl associated to s. sclerotiorum. these markers were used to select bacs contaning single copy sequences for bac ...	2011	21667668
selection on seedling emergence timing and size in an annual plant, helianthus annuus (common sunflower, asteraceae).	variation in seedling emergence timing is considered adaptive over the long term in wild populations, but early emergence can result in a fitness advantage. to explore the adaptive significance of seedling emergence timing, it should be studied under realistic conditions and in the context of other traits that influence fitness.	2011	21653510
the origin and evolution of a recent agricultural weed: population genetic diversity of weedy populations of sunflower (helianthus annuus l.) in spain and france.	the recurrent evolution of crop-related weeds during agricultural history raises serious economic problems and challenging scientific questions. weedy forms of sunflower, a species native from america, have been reported in european sunflower fields for a few decades. in order to understand their origin, we analysed the genetic diversity of a sample of weedy populations from france and spain, and of conventional and ornamental varieties. a crop-specific maternally inherited marker was present in ...	2011	25567998
molecular cloning and characterization of a tocopherol cyclase gene from lactuca sativa (asteraceae).	tocopherol cyclase is a rate-limiting enzyme involved in tocopherol biosynthesis. the full-length cdna encoding tocopherol cyclase (designated as lstc) was cloned from lettuce (lactuca sativa) for the first time by rapid amplification of cdna ends (race) and characterized by means of quantitative rt-pcr. the full-length cdna of lstc was 1675 bp, with an open reading frame of 1521 bp, encoding a tocopherol cyclase protein of 506 amino acids, with a calculated molecular mass of 56.76 kd and an iso ...	2011	21523658
dynamic changes of canopy-scale mesophyll conductance to co₂ diffusion of sunflower as affected by co₂ concentration and abscisic acid.	leaf-level measurements have shown that mesophyll conductance (g(m)) can vary rapidly in response to co₂ and other environmental factors, but similar studies at the canopy-scale are missing. here, we report the effect of short-term variation of co₂ concentration on canopy-scale g(m) and other co₂ exchange parameters of sunflower (helianthus annuus l.) stands in the presence and absence of abscisic acid (aba) in their nutrient solution. g(m) was estimated from gas exchange and on-line carbon isot ...	2011	21029117
an analysis of sequence variability in eight genes putatively involved in drought response in sunflower (helianthus annuus l.).	with the aim to study variability in genes involved in ecological adaptations, we have analysed sequence polymorphisms of eight unique genes putatively involved in drought response by isolation and analysis of allelic sequences in eight inbred lines of sunflower of different origin and phenotypic characters and showing different drought response in terms of leaf relative water content (rwc). first, gene sequences were amplified by pcr on genomic dna from a highly inbred line and their products w ...	2011	21184050
mechanical wounding induces a nitrosative stress by down-regulation of gsno reductase and an increase in s-nitrosothiols in sunflower (helianthus annuus) seedlings.	nitric oxide (no) and related molecules such as peroxynitrite, s-nitrosoglutathione (gsno), and nitrotyrosine, among others, are involved in physiological processes as well in the mechanisms of response to stress conditions. in sunflower seedlings exposed to five different adverse environmental conditions (low temperature, mechanical wounding, high light intensity, continuous light, and continuous darkness), key components of the metabolism of reactive nitrogen species (rns) and reactive oxygen ...	2011	21172815
floret initiation, tissue expansion and carbon availability at the meristem of the sunflower capitulum as affected by water or light deficits.	• the co-ordination between floret initiation and meristem expansion, and their relationships with carbon availability, were studied and quantified in sunflower (helianthus annuus) plants subjected to light or water shortages. • meristem size, number of floret primordia, primordium size, rate of plant biomass accumulation, leaf area, photosynthetic rate, and soluble sugar content in the capitulum were measured until completion of floret initiation. • although treatments differentially affected t ...	2011	20831646
contributions of flowering time genes to sunflower domestication and improvement.	determining the identity and distribution of molecular changes leading to the evolution of modern crop species provides major insights into the timing and nature of historical forces involved in rapid phenotypic evolution. in this study, we employed an integrated candidate gene strategy to identify loci involved in the evolution of flowering time during early domestication and modern improvement of the sunflower (helianthus annuus). sunflower homologs of many genes with known functions in flower ...	2011	20944017
molecular cloning and expression profile analysis of three sunflower (helianthus annuus) diterpene synthase genes.	ent-kaurene, a key precursor of gibberellins, is formed by the action of two diterpene synthases (ditpss), ent-copalyl diphosphate synthase (cps), and ent-kaurene synthase (ks). the full-length cdnas of cps- (hacps1l) and ks-like (haks2l and haks3l) genes were isolated from sunflower. the amino acid sequences of hacps1l, haks2l, and haks3l exhibit structural features and homology to ditpss of several plant species involved in gibberellin biosynthesis. rt-pcr analysis indicates that the expressio ...	2011	20924785
identification of sunflower (helianthus annuus, asteraceae) hybrids using simple-sequence repeat markers.	hybrid identification of 16 sunflower hybrids was confirmed using simple-sequence repeat methodology. of 20 specific simple-sequence repeat primers, 18 authenticated the purity of these hybrids; the remaining two specific primer pairs gave ambiguous dna fragments. the results indicate that simple-sequence repeat analysis for the identification of hybrids derived from the crossing of different inbred sunflower lines can improve the accuracy of selection, save time and reduce cost.	2011	21268778
[integumenal embryony in cms sunflower line].	in ovules of cultural sunflower (helianthus annuus l.) cms line vir 116, which were pollinated by wild perennial specie h. occidentalis, the integumentary embryos have been revealed. they are found out at 7-9 days after pollination in embryo sacs where there was no normal fertilization. the integumentary embryos arise from somatic cells of a parent organism and their occurrence can be one of the reasons matroclinal inheritances at interspecific hybrids.	2011	21268368
photosynthesis-dependent and -independent responses of stomata to blue, red and green monochromatic light: differences between the normally oriented and inverted leaves of sunflower.	the effects of growth light environment on stomatal light responses were analyzed. we inverted leaves of sunflower (helianthus annuus) for 2 weeks until their full expansion, and measured gas exchange properties of the adaxial and abaxial sides separately. the sensitivity to light assessed as the increase in stomatal conductance was generally higher in the abaxial stomata than in the adaxial stomata, and these differences could not be completely changed by the inversion treatment. we also treate ...	2011	21257606
response to imazapyr and dominance relationships of two imidazolinone-tolerant alleles at the ahasl1 locus of sunflower.	imisun and clplus are two imidazolinone (imi) tolerance traits in sunflower (helianthus annuus l.) determined by the expression of different alleles at the same locus, ahasl1-1 and ahasl1-3, respectively. this paper reports the level of tolerance expressed by plants containing both alleles in a homozygous, heterozygous and in a heterozygous stacked state to increasing doses of imi at the enzyme and whole plant levels. six genotypes of the ahasl1 gene were compared with each other in three differ ...	2012	21964993
reconciling extremely strong barriers with high levels of gene exchange in annual sunflowers.	in several cases, estimates of gene flow between species appear to be higher than we might predict given the strength of interspecific barriers separating these species pairs. however, as far as we are aware, detailed measurements of reproductive isolation have not previously been compared with a coalescent-based assessment of gene flow. here, we contrast these two measures in two species of sunflower, helianthus annuus and h. petiolaris. we quantified the total reproductive barrier strength bet ...	2012	22519784
cynarin-rich sunflower (helianthus annuus) sprouts possess both antiglycative and antioxidant activities.	the present study examined the antiglycative and antioxidant properties of four edible sprouts popular in chinese markets. in a protein-reducing sugar model, the sunflower sprout helianthus annuus exhibited the strongest inhibitory effects against the formation of advanced glycation end products (ages). at a concentration of 1.0 mg/ml, its inhibitory rate achieved 83.29%, which is stronger than that of aminoguanidine (1 mm), a well-known synthetic antiglycative agent (with an inhibitory rate of ...	2012	22394088
capture of sunflower seedlings lipase using polyclonal antibodies.	true lipolytic activity is observed in different subcellular fractions of germinating sunflower seedlings (helianthus annuus l.) in delipidated oleosomes and microsomes. triacylglycerol lipase (ec. 3.1.1.3) catalyses the first catabolic step of lipolysis. to our knowledge, this plant lipase has not yet been identified. our aim was to develop a method to collect the lipase for further studies. an immunological method was used to capture sunflower seedling lipase from oleosomes and microsomes. thi ...	2012	22309314
snp discovery and development of a high-density genotyping array for sunflower.	recent advances in next-generation dna sequencing technologies have made possible the development of high-throughput snp genotyping platforms that allow for the simultaneous interrogation of thousands of single-nucleotide polymorphisms (snps). such resources have the potential to facilitate the rapid development of high-density genetic maps, and to enable genome-wide association studies as well as molecular breeding approaches in a variety of taxa. herein, we describe the development of a snp ge ...	2012	22238659
the population genomics of sunflowers and genomic determinants of protein evolution revealed by rnaseq.	few studies have investigated the causes of evolutionary rate variation among plant nuclear genes, especially in recently diverged species still capable of hybridizing in the wild. the recent advent of next generation sequencing (ngs) permits investigation of genome wide rates of protein evolution and the role of selection in generating and maintaining divergence. here, we use individual whole-transcriptome sequencing (rnaseq) to refine our understanding of the population genomics of wild specie ...	2012	24832509
a study on the waste metal remediation using floriculture at east calcutta wetlands, a ramsar site in india.	use of specific plant species in remediation of heavy metal-contaminated soil and water was a promising eco-friendly technology. the present study indicated the possibilities of phytoremediation of metal-contaminated (namely ca, cr, mn, fe, cu, zn, and pb) soil by using plant species important for floriculture of east calcutta wetlands, a ramsar site at the eastern fringe of calcutta city. plant species like sunflower (helianthus annuus), marigold (tagetes patula), and cock's comb (celocia crist ...	2012	21960361
influence of copper on root exudate patterns in some metallophytes and agricultural plants.	a hydroponic experiment was carried out to determine the root exudation patterns in two cu-metallophytes (oenothera picensis and imperata condensata) and two agricultural plants (lupinus albus and helianthus annuus). plants were grown in nutrient solution at increasing cu doses (0, 0.125, 0.25, 0.5, 1 and 2mgcul(-1)), and plant growth, root elongation, cu accumulation and root exudates were measured. all plants showed a decrease of over 60% in root elongation at the highest cu supply level, bein ...	2012	21937112
biological activities and some physicochemical properties of sunflower honeys collected from the thrace region of turkey.	honey is a sweet food made by bees using nectar from flowers. its quality depends on a number of factors, such as floral type, ph, moisture, free acidity, diastase activity, invert sugar and sucrose. the aim of the study is to examine the qualities of 50 sunflower honey (helianthus annuus l.) collected from the thrace region of turkey, in terms of melissopalynological analysis, important chemical parameters and antioxidant activities . the total phenolic content of the honey samples was determin ...	2012	24261112
development of an ultra-dense genetic map of the sunflower genome based on single-feature polymorphisms.	the development of ultra-dense genetic maps has the potential to facilitate detailed comparative genomic analyses and whole genome sequence assemblies. here we describe the use of a custom affymetrix genechip containing nearly 2.4 million features (25 bp sequences) targeting 86,023 unigenes from sunflower (helianthus annuus l.) and related species to test for single-feature polymorphisms (sfps) in a recombinant inbred line (ril) mapping population derived from a cross between confectionery and o ...	2012	23284684
accumulation of cadmium, zinc, and copper by helianthus annuus l.: impact on plant growth and uptake of nutritional elements.	we investigated the effects on physiological response, trace elements and nutrients accumulation of sunflower plants grown in soil contaminated with: 5 mg kg(-1) of cd; 5 and 300 mg kg(-1) of cd and zn, respectively; 5, 300, and 400 mg kg(-1) of cd, zn, and cu, respectively. contaminants applied did not produce large effects on growth, except in cd-zn-cu treatment in which leaf area and total dry matter were reduced, by 15%. the contamination with cd alone did not affect neither growth nor physi ...	2012	22567714
phytoremediation potential of helianthus annuus l in sewage-irrigated indo-gangetic alluvial soils.	the study of phytoremediation potential of helianthus annuus l was conducted in the sewage-irrigated indo-gangetic alluvial soils, india. calcium @ 1.0% and zn @ 40 ppm enhanced the yield of h. annuus l and minimized the toxicity of cr in the investigated soils. the study indicated that h. annuus l is highly sensitive to cr and zn in terms of metallic pollution; and may be used as indicator plant. for cr-phytoremediation, humic acid treatment @ 500 ml/acre induced the cr-accumulation in roots (p ...	2012	22567708
root biomass response to foliar application of imazapyr for two imidazolinone tolerant alleles of sunflower (helianthus annuus l.).	imisun and clplus are two imidazolinone tolerance traits in sunflower (helianthus annuus l.) determined by the expression of two alleles at the locus ahasl1. both traits differed in their tolerance level to imazapyr -a type of imidazolinone herbicide- when aboveground biomass is considered, but the concomitant herbicide effect over the root system has not been reported. the objective of this work was to quantify the root biomass response to increased doses of imazapyr in susceptible (ahasl1/ahas ...	2012	23226083
reduced drought tolerance during domestication and the evolution of weediness results from tolerance-growth trade-offs.	the increased reproductive potential, size, shoot allocation, and growth rate of weedy plants may result from reduced resource allocation to other aspects of plant growth and defense. to investigate whether changes in resource allocation occurred during domestication or the evolution of weediness, we compared the mycorrhizal responsiveness, growth, and drought tolerance of nine native ruderal, nine agriculturally weedy (four u.s. weedy and five australian weedy), and 14 domesticated populations ...	2012	23206138
an extracellular lipid transfer protein is relocalized intracellularly during seed germination.	plant lipid transfer proteins (ltps) constitute a family of small proteins recognized as being extracellular. in agreement with this notion, several lines of evidence have shown the apoplastic localization of haap10, a ltp from helianthus annuus dry seeds. however, haap10 was recently detected intracellularly in imbibing seeds. to clarify its distribution, immunolocalization experiments were performed during the course of germination and confirmed its intracellular localization upon early seed i ...	2012	23162115
acetohydroxyacid synthase (ahas) in vivo assay for screening imidazolinone-resistance in sunflower (helianthus annuus l.).	the objective of this work was to evaluate the in vivo acetohydroxyacid synthase (ahas) activity response to imidazolinones and its possible use as a selection method for evaluating ahas inhibitor resistance. in vivo ahas assay and the comparison of parameters from dose-response curves have been used as a valid tool for comparing sunflower lines and hybrids differing in imidazolinone resistance. the sunflower resistant genotypes evaluated here were 100-fold and 20-fold more resistant compared wi ...	2012	23123550
development, characterization and experimental validation of a cultivated sunflower (helianthus annuus l.) gene expression oligonucleotide microarray.	oligonucleotide-based microarrays with accurate gene coverage represent a key strategy for transcriptional studies in orphan species such as sunflower, h. annuus l., which lacks full genome sequences. the goal of this study was the development and functional annotation of a comprehensive sunflower unigene collection and the design and validation of a custom sunflower oligonucleotide-based microarray. a large scale est (>130,000 ests) curation, assembly and sequence annotation was performed using ...	2012	23110046
morphological variation of mutant sunflowers (helianthus annuus) induced by space flight and their genetic background detection by ssr primers.	after sunflower seeds were exposed to space conditions, various mutant plants were screened from the descendent plants. the morphological characters of plants changed in flower color from golden to yellow, light yellow, or even to yellowish green. the ligulate petals of the unisexual floret broadened, or became thin, while the short tubular petals of bisexual floret elongated to some extent, or even turned into semi-ligulate petals or ligulate petals, making the phenotype of the whole infloresce ...	2012	23079831
a gene-phenotype network based on genetic variability for drought responses reveals key physiological processes in controlled and natural environments.	identifying the connections between molecular and physiological processes underlying the diversity of drought stress responses in plants is key for basic and applied science. drought stress response involves a large number of molecular pathways and subsequent physiological processes. therefore, it constitutes an archetypical systems biology model. we first inferred a gene-phenotype network exploiting differences in drought responses of eight sunflower (helianthus annuus) genotypes to two drought ...	2012	23056196
gene expression assays for actin, ubiquitin, and three microsatellite-encoding genes in helianthus annuus (asteraceae).	the "tuning knob" model of king et al. (endeavor 21: 36-40, 1997) postulates that microsatellite mutations can alter phenotypes in a stepwise fashion. some proposed mechanisms involve regulation of gene expression. to study the effect of microsatellites harbored in untranslated regions on gene expression in helianthus annuus, we have developed taqman assays for three microsatellite-encoding genes, and two constitutively expressed genes, actin and ubiquitin, to serve as standards.	2012	22935362
total synthesis of natural enantiomers of heliespirones a and c via the diastereoselective intramolecular hosomi-sakurai reaction.	a full account of the development of a novel type of the intramolecular hosomi-sakurai reactions of the substrates with a p-benzoquinone and an allylsilane moieties connected by an ether linkage is described. this transformation proceeds via an addition-elimination sequence and provides the products with two stereogenic centers through a 1,3(or 1,4)-asymmetric induction in good to excellent diastereoselectivities. a reasonable mechanistic possibility for the reaction, determination of the stereo ...	2012	22924604
development of a 10,000 locus genetic map of the sunflower genome based on multiple crosses.	genetic linkage maps have the potential to facilitate the genetic dissection of complex traits and comparative analyses of genome structure, as well as molecular breeding efforts in species of agronomic importance. until recently, the majority of such maps was based on relatively low-throughput marker technologies, which limited marker density across the genome. the availability of high-throughput genotyping technologies has, however, made possible the efficient development of high-density genet ...	2012	22870395
role of recently evolved mirna regulation of sunflower hawrky6 in response to temperature damage.	micrornas (mirnas) are small 21-nucleotide rnas that post-transcriptionally regulate gene expression. mir396 controls leaf development by targeting grf and bhlh transcription factors in arabidopsis. wrky transcription factors, unique to plants, have been identified as mediating varied stress responses. the sunflower (helianthus annuus) hawrky6 is a particularly divergent wrky gene exhibiting a putative target site for the mir396. a possible post-transcriptional regulation of hawrky6 by mir396 wa ...	2012	22846054
growth under elevated atmospheric co(2) concentration accelerates leaf senescence in sunflower (helianthus annuus l.) plants.	some morphogenetic and metabolic processes were sensitive to a high atmospheric co(2) concentration during sunflower primary leaf ontogeny. young leaves of sunflower plants growing under elevated co(2) concentration exhibited increased growth, as reflected by the high specific leaf mass referred to as dry weight in young leaves (16 days). the content of photosynthetic pigments decreased with leaf development, especially in plants grown under elevated co(2) concentrations, suggesting that high co ...	2012	22818664
the tolerance efficiency of panicum maximum and helianthus annuus in tnt-contaminated soil and nzvi-contaminated soil.	this study was designed to compare the initial method for phytoremediation involving germination and transplantation. the study was also to determine the tolerance efficiency of panicum maximum (purple guinea grass) and helianthus annuus (sunflower) in tnt-contaminated soil and nzvi-contaminated soil. it was found that the transplantation of panicum maximum and helianthus annuus was more suitable than germination as the initiate method of nano-phytoremediation potting test. the study also showed ...	2012	22702809
the sunflower (helianthus annuus l.) genome reflects a recent history of biased accumulation of transposable elements.	aside from polyploidy, transposable elements are the major drivers of genome size increases in plants. thus, understanding the diversity and evolutionary dynamics of transposable elements in sunflower (helianthus annuus l.), especially given its large genome size (∼3.5 gb) and the well-documented cases of amplification of certain transposons within the genus, is of considerable importance for understanding the evolutionary history of this emerging model species. by analyzing approximately 25% of ...	2012	22691070
response of sunflower (helianthus annuus l.) leaf surface defenses to exogenous methyl jasmonate.	helianthus annuus, the common sunflower, produces a complex array of secondary compounds that are secreted into glandular trichomes, specialized structures found on leaf surfaces and anther appendages of flowers. the primary components of these trichome secretions are sesquiterpene lactones (stl), a diverse class of compounds produced abundantly by the plant family compositae and believed to contribute to plant defense against herbivory. we treated wild and cultivated h. annuus accessions with e ...	2012	22623991
evidence of selection on fatty acid biosynthetic genes during the evolution of cultivated sunflower.	the identification of genes underlying the phenotypic transitions that took place during crop evolution, as well as the genomic extent of resultant selective sweeps, is of great interest to both evolutionary biologists and applied plant scientists. in this study, we report the results of a molecular evolutionary analysis of 11 genes that underlie fatty acid biosynthesis and metabolism in wild and cultivated sunflower (helianthus annuus). seven of these 11 genes showed evidence of selection at th ...	2012	22580969
molecular cloning and biochemical characterization of three phosphoglycerate kinase isoforms from developing sunflower (helianthus annuus l.) seeds.	three cdnas encoding different phosphoglycerate kinase (pgk, ec 2.7.2.3) isoforms, two cytosolic (hacpgk1 and hacpgk2) and one plastidic (happgk), were cloned and characterized from developing sunflower (helianthus annuus l.) seeds. the expression profiles of these genes showed differences in heterotrophic tissues, such as developing seeds and roots, where hacpgk1 was predominant, while happgk was highly expressed in photosynthetic tissues. the cdnas were expressed in escherichia coli, and the c ...	2012	22552275
genetics and mapping of the r₁₁ gene conferring resistance to recently emerged rust races, tightly linked to male fertility restoration, in sunflower (helianthus annuus l.).	sunflower oil is one of the major sources of edible oil. as the second largest hybrid crop in the world, hybrid sunflowers are developed by using the pet1 cytoplasmic male sterility system that contributes to a 20 % yield advantage over the open-pollinated varieties. however, sunflower production in north america has recently been threatened by the evolution of new virulent pathotypes of sunflower rust caused by the fungus puccinia helianthi schwein. rf ann-1742, an 'ha 89' backcross restorer li ...	2012	22610307
root colonization and growth promotion of sunflower (helianthus annuus l.) by phosphate solubilizing enterobacter sp. fs-11.	an enterobacter sp. fs-11 was isolated from sunflower rhizosphere, identified on the basis of 16s rrna gene sequence analysis (genebank accession no. gq179978) and studied for its root colonization and growth promotion ability in sunflower. morphologically, it was rod shaped gram-negative, motile bacterium, producing 4.5 μg ml(-1) indole acetic acid in tryptophan-supplemented medium. it utilized 27 out of 95 substrates in biolog gn2 micro plate system. it was able to convert insoluble tri-calciu ...	2012	22806201
low temperature acclimation of photosynthetic capacity and leaf morphology in the context of phloem loading type.	carbon export from leaf mesophyll to sugar-transporting phloem occurs via either an apoplastic (across the cell membrane) or symplastic (through plasmodesmatal cell wall openings) pathway. herbaceous apoplastic loaders generally exhibit an up-regulation of photosynthetic capacity in response to growth at lower temperature. however, acclimation of photosynthesis to temperature by symplastically loading species, whose geographic distribution is particularly strong in tropical and subtropical areas ...	2012	22791016
ileal digestibility of sunflower meal, pea, rapeseed cake, and lupine in pigs.	the standardized ileal digestibility (sid) of cp and aa was evaluated in soybean (glycine max) meal, sunflower (helianthus annuus) meal, rapeseed cake, and field pea (pisum sativum) using 10 pigs and in lupine (lupinus angustifolius) using 7 pigs. pigs were fitted with either a t-cannula or a steered ileocecal valve-cannula. diets contained 170 to 186 g cp/kg dm. endogenous losses of cp and aa were estimated by feeding a n-free diet. the sid was calculated using the average of cr(2)o(3) and tio( ...	2012	23365330
using a plant hormone and a thioligand to improve phytoremediation of hg-contaminated soil from a petrochemical plant.	mercury-contaminated soils from a petrochemical plant in southern italy were investigated to assess the phytoextraction efficiency of crop plants treated with the phytohormone, cytokinine (ck foliar treatment), and with the thioligand, ammonium thiosulfate (ts, soil application). plant biomass, evapotranspiration, hg uptake and distribution in plant tissues following treatment were compared. results indicate the effectiveness of ck in increasing plant biomass and the evapotranspiration rate whil ...	2012	22771350
inheritance and molecular characterization of broad range tolerance to herbicides targeting acetohydroxyacid synthase in sunflower.	ahasl1 is a multilallelic locus where all the induced and natural mutations for herbicide tolerance were described thus far in sunflower (helianthus annuus l.). the allele ahasl1-1 confers moderate tolerance to imidazolinone (imi), ahasl1-2, and ahasl1-3 provides high levels of tolerance solely to sulfonylurea (su) and imi, respectively. an argentinean wild sunflower population showing plants with high level of tolerance to either an imi and a su herbicide was discovered and used to develop an i ...	2012	21959907
lapacho tea (tabebuia impetiginosa) extract inhibits pancreatic lipase and delays postprandial triglyceride increase in rats.	earlier work in our laboratory indicated that ethanolic extracts of tabebuia impetiginosa, arctium lappa l., calendula officinalis, helianthus annuus, linum usitatissimum and l. propolis, inhibit pancreatic lipase in vitro. in a follow-up study we assessed their effects on plasma triglycerides in rats fed on a fatty meal. extracts, orlistat or only ethanol were given orally to the rats together with the test meal and the rate of increase of postprandial triglycerides was assessed over 4 h. clear ...	2012	22431070
interactive effects of large- and small-scale sources of feral honey-bees for sunflower in the argentine pampas.	pollinators for animal pollinated crops can be provided by natural and semi-natural habitats, ranging from large vegetation remnants to small areas of non-crop land in an otherwise highly modified landscape. it is unknown, however, how different small- and large-scale habitat patches interact as pollinator sources. in the intensively managed argentine pampas, we studied the additive and interactive effects of large expanses (up to 2200 ha) of natural habitat, represented by untilled isolated "si ...	2012	22303477
enhancing phytoextraction: the effect of chemical soil manipulation on mobility, plant accumulation, and leaching of heavy metals.	for heavy metal-contaminated agricultural land, low-cost, plant-based phytoextraction measures can be a key element for a new land management strategy. when agents are applied into the soil, the solubility of heavy metals and their subsequent accumulation by plants can be increased, and, therefore, phytoextraction enhanced. an overview is given of the state of the art of enhancing heavy metal solubility in soils, increasing the heavy metal accumulation of several high-biomass-yielding and metal- ...	2013	14674516
the determination of phenolic profiles of serbian unifloral honeys using ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry.	polyphenolic profiles of 44 unifloral serbian honeys were analyzed using ultra-high-performance liquid chromatography (uhplc) coupled with hybrid mass spectrometer which combines the linear trap quadrupole (ltq) and orbitrap mass analyzer. rapid uhplc method was developed in combination with a high sensitivity accurate mass scan and a simultaneous data dependent scan. the honey samples were of different botanical origin: acacia (robinia pseudoacacia), sunflower (helianthus annuus), linden (tilia ...	2013	23265452
enhanced accumulation of copper and lead in amaranth (amaranthus paniculatus), indian mustard (brassica juncea) and sunflower (helianthus annuus).	soil contamination by copper (cu) and lead (pb) is a widespread environmental problem. for phytoextraction to be successful and viable in environmental remediation, strategies that can improve plant uptake must be identified. in the present study we investigated the use of nitrogen (n) fertilizer as an efficient way to enhance accumulation of cu and pb from contaminated industrial soils into amaranth, indian mustard and sunflower.	2013	23667546
carbon storage in a heavy clay soil landfill site after biosolid application.	applying organic amendments including biosolids and composts to agricultural land could increase carbon (c) storage in soils and contribute significantly to the reduction of greenhouse gas emissions. although a number of studies have examined the potential value of biosolids as a soil conditioner and nutrient source, there has been only limited work on the impact of biosolid application on c sequestration in soils. the objective of this study was to examine the potential value of biosolids in c ...	2013	23380138
preference of a polyphagous mirid bug, apolygus lucorum (meyer-dür) for flowering host plants.	apolygus lucorum (meyer-dür) (hemiptera: miridae) is one of the most important herbivores in a broad range of cultivated plants, including cotton, cereals, vegetables, and fruit crops in china. in this manuscript, we report on a 6-year long study in which (adult) a. lucorum abundance was recorded on 174 plant species from 39 families from early july to mid-september. through the study period per year, the proportion of flowering plants exploited by adult a. lucorum was significantly greater than ...	2013	23874835
cryopreservation of lipid-rich seeds: effect of moisture content and cooling rate on germination.	the effect of fast and slow cooling on germination of seeds from two brassicaceae species (eruca vesicaria (l.) cav., brassica napus l. var. oleifera (moench) dc cv. bingo) and cypselas from three compositae species (onopordum nervosum boiss., onopordum acanthium l., helianthus annuus l. cv. viky) at different moisture contents was studied. seed lipid content (dry weight basis) ranged from 15% (o. nervosum) to 41% (h. annuus). for each species, seeds with four moisture contents were cryopreserve ...	2013	11788852
evaluation and characterisation of citrullus colocynthis (l.) schrad seed oil: comparison with helianthus annuus (sunflower) seed oil.	the physicochemical properties, fatty acid, tocopherol, thermal properties, (1)h nmr, ftir and profiles of non-conventional oil extracted from citrullus colocynthis (l.) schrad seeds were evaluated and compared with conventional sunflower seed oil. in addition, the antioxidant properties of c. colocynthis seed oil were also evaluated. the oil content of the c. colocynthis seeds was 23.16%. the main fatty acids in the oil were linoleic acid (66.73%) followed by oleic acid (14.78%), palmitic acid ...	2013	23122069
the activity of antioxidant enzymes in response to salt stress in safflower (carthamus tinctorius l.) and sunflower (helianthus annuus l.) seedlings raised from seed treated with chitosan.	salt tolerance is a complex trait which involves the coordinated action of many genes that perform a variety of functions, such as ion sequestration, metabolic adjustment, osmotic adjustment and antioxidative defence. in this article, the growth and the generation and scavenging of reactive oxygen species (ros) under normal (eciw [electrical conductivity of irrigation water] = 0.5 ds m(-1)) and salt stress conditions (eciw = 3.4, 6.1, 8.6 and 10.8 ds m(-1) ) in relation to the priming of seeds o ...	2013	23197363
cadmium accumulation in sunflower plants influenced by arbuscular mycorrhiza.	in order to investigate the cadmium (cd) accumulation patterns and possible alleviation of cd stress by mycorrhization, sunflower plants (helianthus annuus l.) were grown in the presence or absence of cd (20 micromol l(-1)) and inoculated or not inoculated with the arbuscular mycorrhizal fungus (amf) glomus intraradices. no visual symptoms of cd phytotoxicity were observed; nevertheless, in non-mycorrhizal plants the presence of cd decreased plant growth. the addition of cd had no significant ef ...	2013	18709928
rhizosphere colonization and arsenic translocation in sunflower (helianthus annuus l.) by arsenate reducing alcaligenes sp. strain dhal-l.	in the present study, six arsenic-resistant strains previously isolated were tested for their plant growth promoting characteristics and heavy metal resistance, in order to choose one model strain as an inoculum for sunflower plants in pot experiments. the aim was to investigate the effect of arsenic-resistant strain on sunflower growth and on arsenic uptake from arsenic contaminated soil. based on plant growth promoting characteristics and heavy metal resistance, alcaligenes sp. strain dhal-l w ...	2013	23632906
bioaugmentation with cadmium-resistant plant growth-promoting rhizobacteria to assist cadmium phytoextraction by helianthus annuus.	micrococcus sp. mu1 and klebsiella sp. bam1, the cadmium-resistant plant growth-promoting rhizobacteria (pgpr), produce high levels of indole-3-acetic acid (iaa) during the late stationary phase of their growth. the ability of pgpr to promote root elongation, plant growth and cadmium uptake in sunflowers (helianthus annuus) was evaluated. both species of bacteria were able to remove cadmium ions from an aqueous solution and enhanced cadmium mobilization in contaminated soil. micrococcus sp. and ...	2013	23478127
laser ablation (imaging) for mapping and determining se and s in sunflower leaves.	qualitative and quantitative methods were developed for selenium and sulphur mapping/quantification in sunflower (helianthus annuus l.) leaves. the plants were grown for 50 days in a greenhouse, being divided into two groups, one irrigated with only deionized water and the other treated with a daily dose of 7.58 mg of na(2)seo(3). leaves were collected during the growth period for directly evaluating the distribution of se and s in these structures. for quantification analysis, pellets were prod ...	2013	23235562
bsta: a targeted approach combines bulked segregant analysis with next- generation sequencing and de novo transcriptome assembly for snp discovery in sunflower.	sunflower belongs to the largest plant family on earth, the genomically poorly explored compositae. downy mildew plasmopara halstedii (farlow) berlese & de toni is one of the major diseases of cultivated sunflower (helianthus annuus l.). in the search for new sources of downy mildew resistance, the locus pl(arg)on linkage group 1 (lg1) originating from h. argophyllus is promising since it confers resistance against all known races of the pathogen. however, the mapping resolution in the pl(arg) r ...	2013	24330545
reappraisal of the genus alternariaster (dothideomycetes).	alternariaster was erected in 2007 to accommodate alternaria helianthi, a fungal species known to cause leaf spots on helianthus annuus (sunflower). it was segregated from alternaria based on conidial morphology. recently an unknown alternaria-like dematiaceous fungus was found associated with leaf spots on bidens sulphurea (yellow cosmos) in brazil. based on a multi-gene phylogeny of parts of the its and lsu genes, this fungus was placed within the leptosphaeriaceae with alternariaster helianth ...	2013	24761036
comprehensive protein-based artificial microrna screens for effective gene silencing in plants.	artificial microrna (amirna) approaches offer a powerful strategy for targeted gene manipulation in any plant species. however, the current unpredictability of amirna efficacy has limited broad application of this promising technology. to address this, we developed epitope-tagged protein-based amirna (etpamir) screens, in which target mrnas encoding epitope-tagged proteins were constitutively or inducibly coexpressed in protoplasts with amirna candidates targeting single or multiple genes. this ...	2013	23645631
interrelationship between viscosity and electrical properties for edible oils.	electrical properties of edible oil depend upon its total polar component, temperature and the frequency of the applied voltage. dielectric constant, dielectric loss tangent and electrical conductivity were measured for cottonseed (gossypium sp.), ground nut (apios americana), mustard (brasicca compestriss) and sun flower (helianthus annuus) oils in the temperature range of 20 to 100°c so as to assess the potential of their applicability for assessing the quality of oils. viscosity of the oils i ...	2013	24425951
evaluation of cover crops with potential for use in anaerobic soil disinfestation (asd) for susceptibility to three species of meloidogyne.	several cover crops with potential for use in tropical and subtropical regions were assessed for susceptibility to three common species of root-knot nematode, meloidogyne arenaria, m. incognita, and m. javanica. crops were selected based on potential use as organic amendments in anaerobic soil disinfestation (asd) applications. nematode juvenile (j2) numbers in soil and roots, egg production, and host plant root galling were evaluated on arugula (eruca sativa, cv. nemat), cowpea (vigna unguicula ...	2013	24379486
complete chloroplast genome sequences of mongolia medicine artemisia frigida and phylogenetic relationships with other plants.	artemisia frigida willd. is an important mongolian traditional medicinal plant with pharmacological functions of stanch and detumescence. however, there is little sequence and genomic information available for artemisia frigida, which makes phylogenetic identification, evolutionary studies, and genetic improvement of its value very difficult. we report the complete chloroplast genome sequence of artemisia frigida based on 454 pyrosequencing.	2013	23460871
genetic mapping of rust resistance genes in confection sunflower line ha-r6 and oilseed line rha 397.	few widely effective resistance sources to sunflower rust, incited by puccinia helianthi schwein., have been identified in confection sunflower (helianthus annuus l.). the usda inbred line ha-r6 is one of the few confection sunflower lines resistant to rust. a previous allelism test indicated that rust resistance genes in ha-r6 and rha 397, an oilseed-type restorer line, are either allelic or closely linked; however, neither have been characterized nor molecularly mapped. the objectives of this ...	2013	23719761
molecular mapping of a sunflower rust resistance gene from har6.	sunflower rust, caused by puccinia helianthi schw., can result in significant yield losses in cultivated sunflower (helianthus annuus l. var. macrocarpus ckll.). har6 is a germplasm population resistant to most predominant rust races. the objectives of this study were to map the resistance factor present in har6 (r har6 ), and to provide and validate molecular tools for the identification of this gene for marker assisted selection purposes. virulence reaction of seedlings for the f2 population a ...	2013	23641190
molecular tagging of a novel rust resistance gene r(12) in sunflower (helianthus annuus l.).	sunflower production in north america has recently suffered economic losses in yield and seed quality from sunflower rust (puccinia helianthi schwein.) because of the increasing incidence and lack of resistance to new rust races. rha 464, a newly released sunflower male fertility restorer line, is resistant to both of the most predominant and most virulent rust races identified in the northern great plains of the usa. the gene conditioning rust resistance in rha 464 originated from wild helianth ...	2013	22907633
antioxidant and antimicrobial properties of monofloral bee pollen.	the main aim of this study was to determine antioxidant properties and antibacterial activity of monofloral bee pollen samples to pathogenic bacteria. these samples were collected in different localities in slovakia. the antioxidant properties of examined plant species were different and decreasing in the following order: brassica napus subsp. napus l > papaver somniferum l. > helianthus annuus l. the antimicrobial effect of the bee product samples were tested by using the agar well diffusion me ...	2013	23305281
suitability of different pollen as alternative food for the predatory mite amblyseius swirskii (acari, phytoseiidae).	the predacious mite amblyseius swirskii athias-henriot is used as a biological control agent against various pests in greenhouses. pollen offered as supplementary food is reported to improve their fast establishment and performance. however, the nutritional suitability of different pollens for a. swirskii is not sufficiently known yet. pollens of 21 plant species were offered to the mites as exclusive food during preimaginal development. preimaginal mortality and developmental time have been ass ...	2013	23670826
seed germination-influencing bioactive secondary metabolites secreted by the endophyte cladosporium cladosporioides lwl5.	the present study was aimed to isolate bioactive metabolites produced by a fungal endophyte from helianthus annuus, capsicum annuum, and cucumis sativus and to assess their role in seed germination. culture filtrate of the endophyte ha-3b from h. annuus was significantly inhibitory towards the germination and growth of lettuce seeds. ha-3b was identified as cladosporium cladosporioides lwl5 through molecular techniques. different concentrations (100, 500 and 1000 ppm) of the ethyl acetate extrac ...	2013	24352011
endogenous ascorbate restrains apoplastic peroxidase activity during sunflower leaf development.	several apoplastic enzymes have been implicated in the control of elongation growth of plant cells. among them, peroxidases contribute to both loosening and stiffening of the cell wall. they appear to be regulated by various mechanisms, including the action of extracellular inhibitors. to obtain evidence of the role of the enzyme-inhibitor interaction during leaf development, the intercellular washing fluids from helianthus annuus leaves of different ages were isolated using standard methods of ...	2013	23043732
malic acid or orthophosphoric acid-heat treatments for protecting sunflower (helianthus annuus) meal proteins against ruminal degradation and increasing intestinal amino acid supply.	the protection of sunflower meal (sfm) proteins by treatments with solutions of malic acid (1 m) or orthophosphoric acid (0.67 m) and heat was studied in a 3 × 3 latin-square design using three diets and three rumen and duodenum cannulated wethers. acid solutions were applied to sfm at a rate of 400 ml/kg under continuous mixing. subsequently, treated meals were dried in an oven at 150°c for 6 h. diets (ingested at 75 g/kg bw0.75) were isoproteic and included 40% italian ryegrass hay and 60% con ...	2013	23032153
phenotypic characterization, genetic mapping and candidate gene analysis of a source conferring reduced plant height in sunflower.	reduced height germplasm has the potential to increase stem strength, standability, and also yields potential of the sunflower crop (helianthus annuus l. var. macrocarpus ckll.). in this study, we report on the inheritance, mapping, phenotypic and molecular characterization of a reduced plant height trait in inbred lines derived from the source ddr. this trait is controlled by a semidominant allele, rht1, which maps on linkage group 12 of the sunflower public consensus map. phenotypic effects of ...	2013	22972203
plant response to lead in the presence or absence edta in two sunflower genotypes (cultivated h. annuus cv. 1114 and interspecific line h. annuus × h. argophyllus).	the aim of the present work was to study the response of two sunflower genotypes (cultivated sunflower helianthus annuus cv. 1114 and newly developed genotype h. annuus × helianthus argophyllus) to pb medium-term stress and the role of exogenously applied edta in alleviating pb toxicity in hydroponics. plant growth, morpho-anatomical characteristics of the leaf tissues, electrolyte leakage, total antioxidant activity, free radical scavenging capacity, total flavonoid content, and superoxide dism ...	2013	23135752
how to escape from crop-to-weed gene flow: phenological variation and isolation-by-time within weedy sunflower populations.	the evolution of crop-related weeds may be constrained by recurrent gene flow from the crop. however, flowering time variation within weedy populations may open the way for weed adaptation by allowing some weeds to escape from this constraint. we investigated this link between phenology, gene flow and adaptation in weedy sunflower populations that have recently emerged in europe from crop-wild hybridization. we studied jointly flowering phenology and genetic diversity for 15 microsatellite loci ...	2013	23181709
divergence is focused on few genomic regions early in speciation: incipient speciation of sunflower ecotypes.	early in speciation, as populations undergo the transition from local adaptation to incipient species, is when a number of transient, but potentially important, processes appear to be most easily detected. these include signatures of selective sweeps that can point to asymmetry in selection between habitats, divergence hitchhiking, and associations of adaptive genes with environments. in a genomic comparison of ecotypes of the prairie sunflower, helianthus petiolaris, occurring at great sand dun ...	2013	24033161
interacting duplications, fluctuating selection, and convergence: the complex dynamics of flowering time evolution during sunflower domestication.	changes in flowering time and its regulation by environmental signals have played crucial roles in the evolutionary origin and spread of many cultivated plants. recent investigations into the genetics of flowering time evolution in the common sunflower, helianthus annuus, have provided insight into the historical and mechanistic dynamics of this process. genetic mapping studies have confirmed phenotypic observations that selection on flowering time fluctuated in direction over sunflower's multis ...	2013	23267017
tyrosine nitration provokes inhibition of sunflower carbonic anhydrase (β-ca) activity under high temperature stress.	protein tyrosine nitration is a post-translational modification (ptm) mediated by reactive nitrogen species (rns) and it is a new area of research in higher plants. previously, it was demonstrated that the exposition of sunflower (helianthus annuus l.) seedlings to high temperature (ht) caused both oxidative and nitrosative stress. the nitroproteome analysis under this stress condition showed the induction of 13 tyrosine-nitrated proteins being the carbonic anhydrase (ca) one of these proteins. ...	2013	23266784