Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| interaction of tomato yellow leaf curl virus with diverse betasatellites enhances symptom severity. | the complete nucleotide sequence was determined for a begomovirus isolated from tomato exhibiting leaf curling and yellowing symptoms in tochigi prefecture in japan. the genome organization of this virus was similar to those of other old world monopartite begomoviruses. neither a dna betasatellite nor a dna-b component was detected. it had the highest total nucleotide sequence identity (99%) with tomato yellow leaf curl virus-israel[japan:tosa:2005] (tylcv-il[jr:tos:05]) and tylcv-israel[japan:h ... | 2009 | 19575277 |
| production of biologically active human myelocytokines in plants. | an effective system for expression of human granulocyte and granulocyte macrophage colony-stimulating factors (hg-csf and hgm-csf) in nicotiana benthamiana plants was developed using viral vector based on tobacco mosaic virus infecting cruciferous plants. the genes of target proteins were cloned into the viral vector driven by actin promoter of arabidopsis thaliana. the expression vectors were delivered into plant cells by agroinjection. maximal synthesis rate was detected 5 days after injection ... | 2009 | 19916932 |
| deletions in the repertoire of pseudomonas syringae pv. tomato dc3000 type iii secretion effector genes reveal functional overlap among effectors. | the gamma-proteobacterial plant pathogen pseudomonas syringae pv. tomato dc3000 uses the type iii secretion system to inject ca. 28 avr/hop effector proteins into plants, which enables the bacterium to grow from low inoculum levels to produce bacterial speck symptoms in tomato, arabidopsis thaliana, and (when lacking hopq1-1) nicotiana benthamiana. the effectors are collectively essential but individually dispensable for the ability of the bacteria to defeat defenses, grow, and produce symptoms ... | 2009 | 19381254 |
| cucurbit yellow stunting disorder virus p25 is a suppressor of post-transcriptional gene silencing. | post-transcriptional gene silencing (ptgs) degrades rna in a sequence-specific manner and is utilised by plants as a natural defence mechanism against virus invaders. two members of the genus crinivirus have been reported to encode suppressors and counter ptgs: sweet potato chlorotic stunt virus p22 and tomato chlorosis virus (tocv) p22, coat protein and coat protein minor. using an agrobacterium-mediated transient assay on nicotiana benthamiana wildtype and 16c plants, we screened four cucurbit ... | 2009 | 19540278 |
| in vitro propagation of plant virus using different forms of plant tissue culture and modes of culture operation. | plant virus accumulation was investigated in vitro using three different forms of plant tissue culture. suspended cells, hairy roots and shooty teratomas of nicotiana benthamiana were infected with tobacco mosaic virus (tmv) using the same initial virus:biomass ratio. viral infection did not affect tissue growth or morphology in any of the three culture systems. average maximum virus concentrations in hairy roots and shooty teratomas were similar and about an order of magnitude higher than in su ... | 2009 | 19616595 |
| expression dynamics and ultrastructural localization of epitope-tagged abutilon mosaic virus nuclear shuttle and movement proteins in nicotiana benthamiana cells. | the geminivirus abutilon mosaic virus (abmv) encodes two proteins which are essential for viral spread within plants. the nuclear shuttle protein (nsp) transfers viral dna between the nucleus and cytoplasm, whereas the movement protein (mp) facilitates transport between cells through plasmodesmata and long-distance via phloem. an inducible overexpression system for epitope-tagged nsp and mp in plants yielded unprecedented amounts of both proteins. western blots revealed extensive posttranslation ... | 2009 | 19628237 |
| high-level hiv-1 nef transient expression in nicotiana benthamiana using the p19 gene silencing suppressor protein of artichoke mottled crinckle virus. | in recent years, different hiv antigens have been successfully expressed in plants by either stable transformation or transient expression systems. among hiv proteins, nef is considered a promising target for the formulation of a multi-component vaccine due to its implication in the first steps of viral infection. attempts to express nef as a single protein product (not fused to a stabilizing protein) in transgenic plants resulted in disappointingly low yields (about 0.5% of total soluble protei ... | 2009 | 19930574 |
| identification of beet necrotic yellow vein virus p25 pathogenicity factor-interacting sugar beet proteins that represent putative virus targets or components of plant resistance. | beet necrotic yellow vein virus (bnyvv) induces the most important disease threatening sugar beet. the growth of partially resistant hybrids carrying monogenic dominant resistance genes stabilize yield but are unable to entirely prevent virus infection and replication. p25 is responsible for symptom development and previous studies have shown that recently occurring resistance-breaking isolates possess increased p25 variability. to better understand the viral pathogenicity factor's interplay wit ... | 2009 | 19589075 |
| tomato bushy stunt virus recombination guided by introduced microrna target sequences. | previously we described tomato bushy stunt virus (tbsv) vectors, which retained their capsid protein gene and were engineered with magnesium chelatase (chlh) and phytoene desaturase (pds) gene sequences from nicotiana benthamiana. upon plant infection, these vectors eventually lost the inserted sequences, presumably as a result of recombination. here, we modified the same vectors to also contain the plant mir171 or mir159 target sequences immediately 3' of the silencing inserts. we inoculated n. ... | 2009 | 19640975 |
| suppression of local rna silencing is not sufficient to promote cell-to-cell movement of turnip crinkle virus in nicotiana benthamiana. | the biological relationship between suppression of rna silencing and virus movement poses an intriguing question in virus-plant interactions. here, we have used a local rna silencing assay, based on a movement-deficient turnip crinkle virus tcv/gfpdeltacp, to investigate the influence of silencing suppression by three different viral suppressors: the tcv 38k coat protein (cp), the 126k protein of tobacco mosaic virus (tmv), and p19 of tomato bushy stunt virus (tbsv) on cell-to-cell movement and ... | 2009 | 19568335 |
| molecular characterization and pathogenicity of tomato yellow leaf curl virus in china. | several tomato production regions in china were surveyed for tomato yellow leaf curl disease (tylcd), and 31 tomato leaf samples showing tylcd-like symptoms were collected. the partial or full-length genomes of these isolates were sequenced and tomato yellow leaf curl virus (tylcv) was detected in shanghai, zhejiang, jiangsu shandong and hebei provinces of china. the tylcv isolates found in china share high sequence identity ([98%) and have more than 97% sequence identity with tylcvil[ il:reo] ( ... | 2009 | 19590945 |
| turnip mosaic virus rna replication complex vesicles are mobile, align with microfilaments, and are each derived from a single viral genome. | nicotiana benthamiana plants were agroinoculated with an infectious cdna clone of turnip mosaic virus (tumv) that was engineered to express a fluorescent protein (green fluorescent protein [gfp] or mcherry) fused to the viral 6k2 protein known to induce vesicle formation. cytoplasmic fluorescent discrete protein structures were observed in infected cells, corresponding to the vesicles containing the viral rna replication complex. the vesicles were motile and aligned with microfilaments. intracel ... | 2009 | 19656892 |
| immunomodulatory activity of a plant extract containing human papillomavirus 16-e7 protein in human monocyte-derived dendritic cells. | this study reports the immunomodulatory activity on human monocyte derived dendritic cells (mddcs) of a vaccine preparation shown to be effective against an hpv16-related tumour in an animal model. the vaccine is composed of extract from nicotiana benthamiana leaves containing hpv16 e7 protein expressed by a potato virus x-derived vector (nbpvx-e7). the effect of the extract was evaluated on mddc differentiation and maturation by monitoring the phenotypic expression of specific markers. the resu ... | 2009 | 20074460 |
| characterization of the cassava geminivirus transcription activation protein putative nuclear localization signal. | the geminivirus transcription activation protein (trap) localizes to the nucleus and contains a putative nuclear localization signal (nls) ((28)prrrr(32)) on the n-terminus. the role of individual residues of this putative nls on nuclear localization and symptom induction was investigated using trap of east african cassava mosaic cameroon virus (eacmcv). subcellular localization was conducted using the green fluorescent protein (gfp). results showed that the proline residue at position 28 (pro-2 ... | 2009 | 19665038 |
| maintenance of an old world betasatellite by a new world helper begomovirus and possible rapid adaptation of the betasatellite. | begomoviruses (family geminiviridae) cause major losses to crops throughout the tropical regions of the world. begomoviruses originating from the new world (nw) and the old world (ow) are genetically distinct. whereas the majority of ow begomoviruses have monopartite genomes and whereas most of these associate with a class of symptom-modulating satellites (known as betasatellites), the genomes of nw begomoviruses are exclusively bipartite and do not associate with satellites. here, we show for t ... | 2009 | 19570867 |
| subcellular localization of the barley stripe mosaic virus triple gene block proteins. | barley stripe mosaic virus (bsmv) spreads from cell to cell through the coordinated actions of three triple gene block (tgb) proteins (tgb1, tgb2, and tgb3) arranged in overlapping open reading frames (orfs). our previous studies (d. m. lawrence and a. o. jackson, j. virol. 75:8712-8723, 2001; d. m. lawrence and a. o. jackson, mol. plant pathol. 2:65-75, 2001) have shown that each of these proteins is required for cell-to-cell movement in monocot and dicot hosts. we recently found (h.-s. lim, j. ... | 2009 | 19570874 |
| proteolysis of a negative regulator of innate immunity is dependent on resistance genes in tomato and nicotiana benthamiana and induced by multiple bacterial effectors. | rpm1-interacting protein 4 (rin4), a negative regulator of the basal defense response in plants, is targeted by multiple bacterial virulence effectors. we show that rin4 degradation is induced by the effector avrpto from pseudomonas syringae and that this degradation in solanaceous plants is dependent on the resistance protein, pto, a protein kinase, and prf, a nucleotide binding site-leucine-rich repeat protein. our data demonstrate overlap between two of the best-characterized pathways for rec ... | 2009 | 19671880 |
| protein mislocalization in plant cells using a gfp-binding chromobody. | a key challenge in cell biology is to directly link protein localization to function. the green fluorescent protein (gfp)-binding protein, gbp, is a 13-kda soluble protein derived from a llama heavy chain antibody that binds with high affinity to gfp as well as to some gfp variants such as yellow fluorescent protein (yfp). a gbp fusion to the red fluorescent protein (rfp), a molecule termed a chromobody, was previously used to trace in vivo the localization of various animal antigens. in this st ... | 2009 | 19686537 |
| the colletotrichum orbiculare ssd1 mutant enhances nicotiana benthamiana basal resistance by activating a mitogen-activated protein kinase pathway. | plant basal resistance is activated by virulent pathogens in susceptible host plants. a colletotrichum orbiculare fungal mutant defective in the ssd1 gene, which regulates cell wall composition, is restricted by host basal resistance responses. here, we identified the nicotiana benthamiana signaling pathway involved in basal resistance by silencing the defense-related genes required for restricting the growth of the c. orbiculare mutant. only silencing of map kinase kinase2 or of both salicylic ... | 2009 | 19706796 |
| molecular characterization and experimental host range of an isolate of wissadula golden mosaic st. thomas virus. | partial genome segments of a begomovirus were previously amplified from wissadula amplissima exhibiting yellow-mosaic and leaf-curl symptoms in the parish of st. thomas, jamaica and this isolate assigned to a tentative begomovirus species, wissadula golden mosaic st. thomas virus. to clone the complete genome of this isolate of wissadula golden mosaic st. thomas virus, abutting primers were designed to pcr amplify its full-length dna-a and dna-b components. sequence analysis of the complete bego ... | 2009 | 19768650 |
| production and characterization of an orally immunogenic plasmodium antigen in plants using a virus-based expression system. | increasing numbers of plant-made vaccines and pharmaceuticals are entering the late stage of product development and commercialization. despite the theoretical benefits of such production, expression of parasite antigens in plants, particularly those from plasmodium, the causative parasites for malaria, have achieved only limited success. we have previously shown that stable transformation of tobacco plants with a plant-codon optimized form of the plasmodium yoelii merozoite surface protein 4/5 ... | 2009 | 19781007 |
| loss of the plant dead-box protein ise1 leads to defective mitochondria and increased cell-to-cell transport via plasmodesmata. | plants have intercellular channels, plasmodesmata (pd), that span the cell wall to enable cell-to-cell transport of micro- and macromolecules. we identified an arabidopsis thaliana embryo lethal mutant increased size exclusion limit 1 (ise1) that results in increased pd-mediated transport of fluorescent tracers. the ise1 mutants have a higher frequency of branched and twinned pd than wild-type embryos. silencing of ise1 in mature nicotiana benthamiana leaves also leads to increased pd transport, ... | 2009 | 19805190 |
| differential resistance to citrus psorosis virus in transgenic nicotiana benthamiana plants expressing hairpin rna derived from the coat protein and 54k protein genes. | citrus psorosis virus (cpsv), genus ophiovirus, family ophioviridae, is the causal agent of a serious disease affecting citrus trees in many countries. the viral genome consists of three ssrnas of negative polarity. post-transcriptional gene silencing (ptgs), a mechanism of plant defence against viruses, can be induced by transgenic expression of virus-derived sequences encoding hairpin rnas. since the production of transgenic citrus lines and their evaluation would take years, a herbaceous mode ... | 2009 | 19820946 |
| a myosin xi tail domain homologous to the yeast myosin vacuole-binding domain interacts with plastids and stromules in nicotiana benthamiana. | the actin cytoskeleton plays a role in mobility of many different organelles in plant cells, including chloroplasts, mitochondria, golgi, and peroxisomes. while progress has been made in identifying the myosin motors involved in trafficking of various plant organelles, not all of the cargoes mobilized by different members of the myosin xi family have yet been identified. the involvement of myosins in chloroplast positioning and mitochondrial movement was demonstrated by expression of a virus-ind ... | 2009 | 19995734 |
| molecular cloning and characterization of the promoter for the multiple stress-inducible gene bjchi1 from brassica juncea. | we have previously isolated a brassica juncea cdna encoding a novel chitinase bjchi1 with two chitin-binding domains (zhao and chye in plant mol biol 40:1009-1018, 1999). the expression of bjchi1 was highly inducible by methyl jasmonate (meja) treatment, wounding, caterpillar feeding, and pathogenic fungal infection. these observations suggest that the promoter of bjchi1 gene might contain specific cis-acting elements for stress responses. here, we report the cloning and characterization of the ... | 2009 | 19277702 |
| inhibitors of plant invertases do not affect the structurally related enzymes of fructan metabolism. | plant fructan active enzymes (fazys), including the enzymes involved in inulin metabolism, namely sucrose:sucrose 1-fructosyltransferase (1-sst; ec 2.4.1.99), fructan:fructan 1-fructosyltransferase (1-fft; ec 2.4.1.100) and fructan 1-exohydrolase (1-feh; ec 3.2.1.153), are evolutionarily related to acid invertases (ais), that is, plant cell wall invertase (cwi) and vacuolar invertase (vi). acid invertases are post-translationally controlled by proteinaceous inhibitors. whether fazys are subject ... | 2009 | 19037899 |
| agroinoculation of the crinivirus, lettuce infectious yellows virus, for systemic plant infection. | lettuce infectious yellows virus (liyv) is phloem-limited, non-mechanically transmissible, and is transmitted to plants only by bemisia tabaci. here, we developed agroinoculation to deliver liyv to plants thereby obviating the need for b. tabaci. agroinfiltration of rna 1 containing a green fluorescent protein gene into nicotiana benthamiana leaves resulted in subliminal infections, as judged by green fluorescence. agroinfiltration of liyv wild-type rna 1 and 2 constructs resulted in systemic in ... | 2009 | 19632699 |
| melandrium yellow fleck bromovirus infects arabidopsis thaliana and has genomic rna sequence characteristics that are unique among bromoviruses. | melandrium yellow fleck bromovirus (myfv) systemically infected arabidopsis thaliana, although the susceptibility of several a. thaliana accessions to myfv differed from their susceptibility to the other two bromoviruses infecting a. thaliana. we constructed full-length cdna clones of myfv genomic rnas 1, 2, and 3 and determined their complete nucleotide sequences. similar to broad bean mottle bromovirus, (1) the 5'-terminal nucleotide of the myfv genomic rnas was adenine, and (2) the "d-arm" wa ... | 2009 | 19597694 |
| defining the determinants for dual targeting of amino acyl-trna synthetases to mitochondria and chloroplasts. | most of the organellar amino acyl-trna synthetases (aarss) are dually targeted to both mitochondria and chloroplasts using dual targeting peptides (dtps). we have investigated the targeting properties and domain structure of dtps of seven aarss by studying the in vitro and in vivo import of n-terminal deleted constructs of dtps fused to green fluorescent protein. the deletion constructs were designed based on prediction programs, targetp and predotar, as well as logoplots derived from organellar ... | 2009 | 19733576 |
| exploiting knowledge of r/avr genes to rapidly clone a new lz-nbs-lrr family of late blight resistance genes from potato linkage group iv. | in addition to the resistance to phytophthora infestans (rpi) genes rpi-blb1 and rpi-blb2, solanum bulbocastanum appears to harbor rpi-blb3 located at a major late blight resistance locus on lg iv, which also harbors rpi-abpt, r2, r2-like, and rpi-mcd1 in other solanum spp. here, we report the cloning and functional analyses of four rpi genes, using a map-based cloning approach, allele-mining strategy, gateway technology, and transient complementation assays in nicotiana benthamiana. rpi-blb3, r ... | 2009 | 19445588 |
| phytophthora infestans isolates lacking class i ipio variants are virulent on rpi-blb1 potato. | a strategy to control the devastating late blight disease is providing potato cultivars with genes that are effective in resistance to a broad spectrum of phytophthora infestans isolates. thus far, most late blight resistance (r) genes that were introgressed in potato were quickly defeated. in contrast, the rpi-blb1 gene originating from solanum bulbocastanum has performed as an exclusive broad-spectrum r gene for many years. recently, the rxlr effector family ipio was identified to contain avr- ... | 2009 | 19888819 |
| suppression and overexpression of ubiquitin extension protein s27a affects cell proliferation and in vitro regeneration in nicotiana benthamiana. | ubiquitin is a highly conserved 76-amino-acid protein found in all eukaryotic cells. ubiquitin's expression is encoded and expressed as multimeric head-to-tail repeats (polyubiquitins) that are post-translationally cleaved into monomers, or fused with ribosomal proteins s27a and l40. s27a is highly expressed in meristematic tissues, pollen and ovules and its ubiquitin moiety is thought to act as a chaperone in ribosome biogenesis prior to cleavage. this study suggests that the ribosomal protein ... | 2009 | 26493147 |
| cell-to-cell trafficking, subcellular distribution, and binding to coat protein of broad bean wilt virus 2 vp37 protein. | broad bean wilt virus 2 (bbwv 2) is a member of the genus fabavirus of the family comoviridae. to date, a movement protein (mp) of bbwv 2 has not been described. here we demonstrate that the green fluorescent protein (gfp)-vp37 fusion protein can move from initial bombarded cells to neighboring cells in nicotiana benthamiana epidermal leaves. in addition, the gfp-vp37 fusion protein localizes as a halo around the nucleus and as punctate spots on the cell periphery in n. benthamiana epidermal lea ... | 2009 | 19463725 |
| wpp-domain proteins mimic the activity of the hsc70-1 chaperone in preventing mistargeting of rangap1-anchoring protein wit1. | arabidopsis (arabidopsis thaliana) tryptophan-proline-proline (wpp)-domain proteins, wpp1 and wpp2, are plant-unique, nuclear envelope-associated proteins of unknown function. they have sequence similarity to the nuclear envelope-targeting domain of plant rangap1, the gtpase activating protein of the small gtpase ran. wpp domain-interacting tail-anchored protein 1 (wit1) and wit2 are two arabidopsis proteins containing a coiled-coil domain and a c-terminal predicted transmembrane domain. they ar ... | 2009 | 19617588 |
| the arabidopsis thaliana double-stranded rna binding protein drb1 directs guide strand selection from microrna duplexes. | in arabidopsis thaliana (arabidopsis), dicer-like1 (dcl1) functions together with the double-stranded rna binding protein (dsrbp), drb1, to process micrornas (mirnas) from their precursor transcripts prior to their transfer to the rna-induced silencing complex (risc). mirna-loaded risc directs rna silencing of cognate mrnas via argonaute1 (ago1)-catalyzed cleavage. short interefering rnas (sirnas) are processed from viral-derived or transgene-encoded molecules of double-stranded rna (dsrna) by t ... | 2009 | 19861421 |
| transmission studies with cassava brown streak uganda virus (potyviridae: ipomovirus) and its interaction with abiotic and biotic factors in nicotiana benthamiana. | cassava brown streak disease (cbsd), caused by two distinct species, cassava brown streak uganda virus (cbsuv) and cassava brown streak virus (cbsv), is a major constraint to cassava (manihot esculenta crantz) production in africa. absence of infectious clones of cbsuv or cbsv and the lack of efficient means of mechanical transmission of cbsd has hampered laboratory studies of this disease. mechanical transmission, achieved mainly by plant sap inoculation, is a widely used technique for characte ... | 2010 | 20691215 |
| rapid expression of transgenes driven by seed-specific constructs in leaf tissue: dha production. | metabolic engineering of seed biosynthetic pathways to diversify and improve crop product quality is a highly active research area. the validation of genes driven by seed-specific promoters is time-consuming since the transformed plants must be grown to maturity before the gene function can be analysed. | 2010 | 20222981 |
| evaluation of the minimal replication time of cauliflower mosaic virus in different hosts. | though the duration of a single round of replication is an important biological parameter, it has been determined for only few viruses. here, this parameter was determined for cauliflower mosaic virus (camv) in transfected protoplasts from different hosts: the highly susceptible arabidopsis and turnip, and nicotiana benthamiana, where camv accumulates only slowly. four methods of differing sensitivity were employed: labelling of (1) progeny dna and (2) capsid protein, (3) immunocapture pcr,, and ... | 2010 | 19913268 |
| in vivo effects of nbsir silencing on chloroplast development in nicotiana benthamiana. | sulfite reductase (sir) performs dual functions, acting as a sulfur assimilation enzyme and as a chloroplast (cp-) nucleoid binding protein. in this study, we examined the in vivo effects of sir deficiency on chloroplast development in nicotiana benthamiana. virus-induced gene silencing of nbsir resulted in leaf yellowing and growth retardation phenotypes, which were not rescued by cysteine supplementation. nbsir:gfp fusion protein was targeted to chloroplasts and colocalized with cp-nucleoids. ... | 2010 | 20047069 |
| a quantitative real-time pcr method for in planta monitoring of phytophthora infestans growth. | to establish a reliable and rapid protocol to simultaneously obtain high quality dna from an infected host plant and the infecting pathogen. to develop an accurate and sensitive low-cost assay for the quantification and in planta monitoring of phytophthora infestans growth. | 2010 | 21039667 |
| age-related resistance of nicotiana benthamiana against hemibiotrophic pathogen phytophthora infestans requires both ethylene- and salicylic acid-mediated signaling pathways. | phytophthora infestans, the agent of late blight disease of potato, is a hemibiotrophic pathogen with biotrophic action during early infection and necrotrophic in the later stage of colonization. mature nicotiana benthamiana was resistant to p. infestans, whereas relatively young plants were susceptible to this pathogen. young plants became resistant following a pretreatment with acibenzolar-s-methyl, a functional analog of salicylic acid (sa), indicating that susceptibility of young plants is d ... | 2010 | 20687803 |
| plastidial thioredoxin z interacts with two fructokinase-like proteins in a thiol-dependent manner: evidence for an essential role in chloroplast development in arabidopsis and nicotiana benthamiana. | here, we characterize a plastidial thioredoxin (trx) isoform from arabidopsis thaliana that defines a previously unknown branch of plastidial trxs lying between x- and y-type trxs and thus was named trx z. an arabidopsis knockout mutant of trx z had a severe albino phenotype and was inhibited in chloroplast development. quantitative real-time rt-pcr analysis of the mutant suggested that the expressions of genes that depend on a plastid-encoded rna polymerase (pep) were specifically decreased. si ... | 2010 | 20511297 |
| arabidopsis thaliana pattern recognition receptors for bacterial elongation factor tu and flagellin can be combined to form functional chimeric receptors. | the receptor kinase efr of arabidopsis thaliana detects the microbe-associated molecular pattern elf18, a peptide that represents the n terminus of bacterial elongation factor tu. here, we tested subdomains of efr for their importance in receptor function. transient expression of tagged versions of efr and efr lacking its cytoplasmic domain in leaves of nicotiana benthamiana resulted in functional binding sites for elf18. no binding of ligand was found with the ectodomain lacking the transmembra ... | 2010 | 20410299 |
| methods to study pamp-triggered immunity using tomato and nicotiana benthamiana. | understanding the molecular basis of plant responses to pathogen-associated molecular patterns (pamps) is an active area of research in the field of plant-microbe interactions. a growing number of plant genes involved in various steps of pamp-triggered immunity (pti) pathways and microbial factors involved in the elicitation or suppression of pti have been identified. these studies have largely relied on arabidopsis thaliana and, therefore, most of the pti assays have been developed and optimize ... | 2010 | 20615110 |
| multiple regulatory elements in the arabidopsis nia1 promoter act synergistically to form a nitrate enhancer. | to accommodate fluctuating nutrient levels in the soil, plants modulate their metabolism and root development via signaling mechanisms that rapidly reprogram the plant transcriptome. in the case of nitrate, over 1,000 genes are induced or repressed within minutes of nitrate exposure. to identify cis-regulatory elements that mediate these responses, an enhancer screen was performed in transgenic arabidopsis (arabidopsis thaliana) plants. a 1.8-kb promoter fragment from the nitrate reductase gene ... | 2010 | 20668061 |
| interfamily transfer of a plant pattern-recognition receptor confers broad-spectrum bacterial resistance. | plant diseases cause massive losses in agriculture. increasing the natural defenses of plants may reduce the impact of phytopathogens on agricultural productivity. pattern-recognition receptors (prrs) detect microbes by recognizing conserved pathogen-associated molecular patterns (pamps). although the overall importance of pamp-triggered immunity for plant defense is established, it has not been used to confer disease resistance in crops. we report that activity of a prr is retained after its tr ... | 2010 | 20231819 |
| multiple receptor complexes assembled for transmitting clv3 signaling in arabidopsis. | in arabidopsis, the feedback regulatory loop between clavata3 (clv3) signaling pathway and transcription factor, wuschel (wus) plays a significant role in shoot apical meristems (sam) maintenance. previously, clv1/clv2 heterodimers were supposed to perceive and transmit clv3 signaling. recent genetic analysis isolated a novel receptor kinase, coryne (crn), which was found to be involved in the clv3 pathway. therefore, new hypothesis was put forward that crn probably acts with clv2 to transmit cl ... | 2010 | 20220313 |
| a host rna helicase-like protein, atrh8, interacts with the potyviral genome-linked protein, vpg, associates with the virus accumulation complex, and is essential for infection. | the viral genome-linked protein, vpg, of potyviruses is a multifunctional protein involved in viral genome translation and replication. previous studies have shown that both eukaryotic translation initiation factor 4e (eif4e) and eif4g or their respective isoforms from the eif4f complex, which modulates the initiation of protein translation, selectively interact with vpg and are required for potyvirus infection. here, we report the identification of two dead-box rna helicase-like proteins, ppddx ... | 2010 | 19880609 |
| rpk2 is an essential receptor-like kinase that transmits the clv3 signal in arabidopsis. | the shoot apical meristem (sam) is the fundamental structure that is located at the growing tip and gives rise to all aerial parts of plant tissues and organs, such as leaves, stems and flowers. in arabidopsis thaliana, the clavata3 (clv3) pathway regulates the stem cell pool in the sam, in which a small peptide ligand derived from clv3 is perceived by two major receptor complexes, clv1 and clv2-coryne (crn)/suppressor of llp1 2 (sol2), to restrict wuschel (wus) expression. in this study, we use ... | 2010 | 20978082 |
| bean dwarf mosaic virus: a model system for the study of viral movement. | bean dwarf mosaic virus-[colombia:1987] (bdmv-[co:87]) is a single-stranded plant dna virus, a member of the genus begomovirus of the family geminiviridae. | 2010 | 20618704 |
| a mutation in the lettuce infectious yellows virus minor coat protein disrupts whitefly transmission but not in planta systemic movement. | the lettuce infectious yellows virus (liyv) rna 2 mutant p1-5b was previously isolated from bemisia tabaci-transmitted virus maintained in chenopodium murale plants. p1-5b rna 2 contains a single-nucleotide deletion in the minor coat protein (cpm) open reading frame (orf) that is predicted to result in a frameshift and premature termination of the protein. using the recently developed agroinoculation system for liyv, we tested rna 2 containing the p1-5b cpm mutant genotype (agro-pr6-5b) in nicot ... | 2010 | 20861267 |
| a role for chloroplast-localized thylakoid formation 1 (thf1) in bacterial speck disease development. | coronatine (cor), a jasmonate mimic produced by pseudomonas syringae pv. tomato dc3000 (pst dc3000) is required for full virulence of pst dc3000 in tomato and arabidopsis. cor is shown to induce a range of physiological processes including chlorosis, root growth inhibition and anthocyanin accumulation in tomato. to elucidate the host/signaling genes involved in cor-responses, we utilized a forward genetics approach using nicotiana benthamiana and virus-induced gene silencing (vigs) and identifie ... | 2010 | 20339321 |
| the pathogenicity determinant of citrus tristeza virus causing the seedling yellows syndrome maps at the 3'-terminal region of the viral genome. | citrus tristeza virus (ctv) (genus closterovirus, family closteroviridae) causes some of the more important viral diseases of citrus worldwide. the ability to map disease-inducing determinants of ctv is needed to develop better diagnostic and disease control procedures. a distinctive phenotype of some isolates of ctv is the ability to induce seedling yellows (sy) in sour orange, lemon and grapefruit seedlings. in florida, the decline isolate of ctv, t36, induces sy, whereas a widely distributed ... | 2010 | 20078776 |
| identification of tomato phosphatidylinositol-specific phospholipase-c (pi-plc) family members and the role of plc4 and plc6 in hr and disease resistance. | the perception of pathogen-derived elicitors by plants has been suggested to involve phosphatidylinositol-specific phospholipase-c (pi-plc) signalling. here we show that plc isoforms are required for the hypersensitive response (hr) and disease resistance. we characterised the tomato [solanum lycopersicum (sl)] plc gene family. six sl plc-encoding cdnas were isolated and their expression in response to infection with the pathogenic fungus cladosporium fulvum was studied. we found significant reg ... | 2010 | 20088897 |
| a tobamovirus expression vector for agroinfection of legumes and nicotiana. | the highest recombinant protein expression levels in plants have been achieved using tobacco mosaic virus (tmv) vectors via agroinoculation of the tobacco, nicotiana benthamiana. these vectors have been utilized for pharmaceutical protein production and also can serve as rapid gene expression screens for proteonomics. we have constructed a similar vector based on the legume-infecting tobamovirus, sunn hemp mosaic virus (shmv), by deleting the coat protein gene (shmv eliminate coat protein gene o ... | 2010 | 20380855 |
| molecular characterization and infectivity of papaya leaf curl china virus infecting tomato in china. | papaya leaf curl china virus (palcucnv) was previously reported as a distinct begomovirus infecting papaya in southern china. based on molecular diagnostic survey, 13 palcucnv isolates were obtained from tomato plants showing leaf curl symptoms in henan and guangxi provinces of china. complete nucleotide sequences of 5 representative isolates (aj558116, aj558117, aj704604, fn256260, and fn297834) were determined to be 2738-2751 nucleotides, which share 91.7%-97.9% sequence identities with palcuc ... | 2010 | 20104645 |
| s-glycoprotein-like protein regulates defense responses in nicotiana plants against ralstonia solanacearum. | rsrga4 (for ralstonia solanacearum-responsive gene a4) encodes a polypeptide similar to s-locus glycoprotein (sgp) from brassica rapa and sgp-like proteins from ipomoea trifida and medicago truncatula. therefore, we designated rsrga4 as ntsglp (for nicotiana tabacum sgp-like protein) and nbsglp (its nicotiana benthamiana ortholog). nbsglp is expressed in root, leaf, petal, gynoecium, and stamen. expression of nbsglp was strongly induced by inoculation with an avirulent strain of r. solanacearum ... | 2010 | 20118275 |
| a progeny virus from a cucumovirus pseudorecombinant evolved to gain the ability to accumulate its rna-silencing suppressor leading to systemic infection in tobacco. | two isolates of tomato aspermy virus (tav), v-tav and c-tav, can systemically infect nicotiana benthamiana but only c-tav can move systemically in n. tabacum. any pseudorecombinants between the two strains could not move systemically in tobacco as efficiently as c-tav. however, a pseudorecombinant consisting of rnas 1 and 3 of v-tav and rna 2 of c-tav (v1c2v3), which cannot infect tobacco systemically, generated progeny with a mutation in v1 and a recombination in c2 (v1(m)c2(r)v3), enabling the ... | 2010 | 20121454 |
| the tobacco etch virus p3 protein forms mobile inclusions via the early secretory pathway and traffics along actin microfilaments. | plant potyviruses encode two membrane proteins, 6k and p3. the 6k protein has been shown to induce virus replication vesicles. however, the function of p3 remains unclear. in this study, subcellular localization of the tobacco etch virus (tev) p3 protein was investigated in nicotiana benthamiana leaf cells. the tev p3 protein localized on the endoplasmic reticulum (er) membrane and formed punctate inclusions in association with the golgi apparatus. the trafficking of p3 to the golgi was mediated ... | 2010 | 19945728 |
| one-step, zero-background ligation-independent cloning intron-containing hairpin rna constructs for rnai in plants. | *the hairpin-based rna interference (rnai) technique plays an important role in exploring gene function in plants. although there are several methods for making hairpin rna (hprna) constructs, these methods usually need multiple relatively laborious, time-consuming or high-cost cloning steps. here we describe a one-step, zero-background ligation-independent cloning (oz-lic) method for making intron-containing hprna (ihprna) constructs by our vector prnai-lic. *to generate the ihprna constructs w ... | 2010 | 20406406 |
| 2-de proteome maps for the leaf apoplast of nicotiana benthamiana. | we provide 2-d gel reference maps for the apoplastic proteome of nicotiana benthamiana leaves infiltrated or not with the bacterial gene vector agrobacterium tumefaciens. about 90 proteins were analyzed by lc-ms/ms for identification and function assignment. we show, overall, an effective response of the plant to agroinfiltration involving a specific, cell wall maintenance-independent up-regulation of defense protein secretion. the proteome maps described should be a useful tool for systemic stu ... | 2010 | 20422621 |
| green biofactories: recombinant protein production in plants. | until recently, low accumulation levels have been the major bottleneck for plant-made recombinant protein production. however, several breakthroughs have been described in the past few years allowing for very high maccumulation levels, mainly through chloroplast transformation and transient expression, coupled with subcellular targeting and protein fusions. another important factor influencing our ability to use plants for the production of recombinant proteins is the availability of quick and s ... | 2010 | 20423324 |
| p0 proteins of european beet-infecting poleroviruses display variable rna silencing suppression activity. | post-transcriptional gene silencing (ptgs), or rna silencing, is one of the key mechanisms of antiviral defence used by plants. to counter this defence response, viruses produce suppressor proteins that are able to inhibit the ptgs pathway or to interfere with some of its function. the aim of this study was to evaluate the rna silencing suppressor (rss) activity of p0 proteins from selected european isolates of the beet-infecting poleroviruses beet chlorosis virus (bchv) and beet mild yellowing ... | 2010 | 19955562 |
| the hypersensitive response induced by the v2 protein of a monopartite begomovirus is countered by the c2 protein. | a functional analysis of the v2 protein of two monopartite begomoviruses, papaya leaf curl virus (palcuv) and cotton leaf curl kokhran virus (clcukov), has been performed. expression of the v2 gene from a potato virus x (pvx) vector resulted in severe leaf curling followed by a hypersensitive response (hr) in nicotiana benthamiana and n. tabacum, demonstrating that the v2 protein is a pathogenicity determinant and a target of host defence responses. agroinfiltration of a pvx vector expressing th ... | 2010 | 20447273 |
| role of nitric oxide and reactive oxide species in disease resistance to necrotrophic pathogens. | nitric oxide (no) and reactive oxygen species (ros) are important signaling molecules in plant immunity. however, roles of no and ros in disease resistance to necrotrophic pathogens are not fully understood. we have recently demonstrated that no plays a pivotal role in basal defense against botrytis cinerea and the expression of the salicylic acid (sa) -responsive gene pr-1in nicotiana benthamiana. by contrast, ros function negatively in resistance or positively in expansion of disease lesions d ... | 2010 | 20448455 |
| inhibition of sterol biosynthesis reduces tombusvirus replication in yeast and plants. | the replication of plus-strand rna viruses depends on subcellular membranes. recent genome-wide screens have revealed that the sterol biosynthesis genes erg25 and erg4 affected the replication of tomato bushy stunt virus (tbsv) in a yeast model host. to further our understanding of the role of sterols in tbsv replication, we demonstrate that the downregulation of erg25 or the inhibition of the activity of erg25p with an inhibitor (6-amino-2-n-pentylthiobenzothiazole; apb) leads to a 3- to 5-fold ... | 2010 | 20015981 |
| nicotiana tabacum eil2 directly regulates expression of at least one tobacco gene induced by sulphur starvation. | sulphur deficiency severely affects plant growth and their agricultural productivity leading to diverse changes in development and metabolisms. molecular mechanisms regulating gene expression under low sulphur conditions remain largely unknown. atslim1, a member of the ein3-like (eil) family was reported to be a central transcriptional regulator of the plant sulphur response, however, no direct interaction of this protein with any sulphur-responsive promoters was demonstrated. the focus of this ... | 2010 | 20018902 |
| pathogenicity of alternanthera mosaic virus is affected by determinants in rna-dependent rna polymerase and by reduced efficacy of silencing suppression in a movement-competent tgb1. | four biologically active cdna clones were derived from the alternanthera mosaic virus (altmv; genus potexvirus) isolate, altmv-sp, which differ in symptoms in infected nicotiana benthamiana plants. two clones induced necrosis and plant death; a mixture of all four clones induced milder symptoms than altmv-sp. replication of all clones was enhanced by a minimum of fourfold at 15 degrees c. a mixture of clones 4-7 (severe) and 3-1 (mild) was indistinguishable from altmv-sp, but the ratio of 4-7 to ... | 2010 | 20019006 |
| competition between phytophthora infestans effectors leads to increased aggressiveness on plants containing broad-spectrum late blight resistance. | the destructive plant disease potato late blight is caused by the oomycete pathogen phytophthora infestans (mont.) de bary. this disease has remained particularly problematic despite intensive breeding efforts to integrate resistance into cultivated potato, largely because of the pathogen's ability to quickly evolve to overcome major resistance genes. the rb gene, identified in the wild potato species s. bulbocastanum, encodes a protein that confers broad-spectrum resistance to most p. infestans ... | 2010 | 20479869 |
| cloning and molecular analysis of hlbzip1 and hlbzip2 transcription factors putatively involved in the regulation of the lupulin metabolome in hop (humulus lupulus l.). | hop (humulus lupulus l.), the essential source of beer flavor is of interest from a medicinal perspective in view of its high content in health-beneficial terpenophenolics including prenylflavonoids. the dissection of biosynthetic pathway(s) of these compounds in lupulin glands, as well as its regulation by transcription factors (tfs), is important for efficient biotechnological manipulation of the hop metabolome. tfs of the bzip class were preselected from the hop transcriptome using a cdna-afl ... | 2010 | 20028133 |
| tomato 14-3-3 protein 7 positively regulates immunity-associated programmed cell death by enhancing protein abundance and signaling ability of mapkkk {alpha}. | programmed cell death (pcd) is triggered when pto, a ser-thr protein kinase, recognizes either the avrpto or avrptob effector from pseudomonas syringae pv tomato. this pcd requires mitogen-activated protein kinase kinase kinase (mapkkk alpha ) as a positive regulator in tomato (solanum lycopersicum) and nicotiana benthamiana. to examine how pcd-eliciting activity of the tomato mapkkk alpha protein is regulated, we screened for mapkkk alpha -interacting proteins in tomato and identified a 14-3-3 ... | 2010 | 20061552 |
| a plastid-targeted heat shock cognate 70kda protein interacts with the abutilon mosaic virus movement protein. | the movement protein (mp) of bipartite geminiviruses facilitates cell-to-cell as well as long-distance transport within plants and influences viral pathogenicity. yeast two-hybrid assays identified a chaperone, the nuclear-encoded and plastid-targeted heat shock cognate 70kda protein (cphsc70-1) of arabidopsis thaliana, as a potential binding partner for the abutilon mosaic virus (abmv) mp. in planta, bimolecular fluorescence complementation (bifc) analysis showed cphsc70-1/mp complexes and mp h ... | 2010 | 20193958 |
| optimisation of the purification process of a tumour-targeting antibody produced in n. benthamiana using vacuum-agroinfiltration. | it was previously demonstrated that the tumour-targeting antibody mab h10 can be transiently expressed and purified at high levels in nicotiana benthamiana by using a vacuum-agroinfiltration system boosted by the use of a virus silencing suppressor protein. scope of this work was to analyse different steps of protein extraction from agroinfiltrated leaves to optimise the purification process of the secretory mab h10 providing new insights in the field of large-scale plant production. two differe ... | 2010 | 20229286 |
| changes induced by the pepper mild mottle tobamovirus on the chloroplast proteome of nicotiana benthamiana. | we have analyzed the chloroplast proteome of nicotiana benthamiana using two-dimensional gel electrophoresis and mass spectrometry followed by a database search. in order to improve the resolution of the two-dimensional electrophoresis gels, we have made separate maps for the low and the high ph range. at least 200 spots were detected. we identified 72 polypeptides, some being isoforms of different multiprotein families. in addition, changes in this chloroplast proteome induced by the infection ... | 2010 | 19823941 |
| evolutionary trajectory of turnip mosaic virus populations adapting to a new host. | little is known about how some plant viruses establish successful cross-species transmission whilst others do not; the genetic basis for adaptation is largely unknown. this study investigated the genetic changes that occurred using the progeny of an infectious clone, p35tunos, derived from the turnip mosaic virus (tumv) uk 1 isolate, which has a brassica host type, but rarely infects raphanus systemically and then only asymptomatically. the genetic trajectory leading to viral adaptation was stud ... | 2010 | 19906944 |
| a virus-induced gene silencing screen identifies a role for thylakoid formation1 in pseudomonas syringae pv tomato symptom development in tomato and arabidopsis. | pseudomonas syringae pv tomato dc3000 (pst dc3000), which causes disease in tomato (solanum lycopersicum) and arabidopsis (arabidopsis thaliana), produces coronatine (cor), a non-host-specific phytotoxin. cor, which functions as a jasmonate mimic, is required for full virulence of pst dc3000 and for the induction of chlorosis in host plants. previous genetic screens based on insensitivity to cor and/or methyl jasmonate identified several potential targets for cor and methyl jasmonate. in this st ... | 2010 | 19915014 |
| biosynthesis of glucosinolates--gene discovery and beyond. | glucosinolates are sulfur-rich secondary metabolites characteristic of the brassicales order with important biological and economic roles in plant defense and human nutrition. application of systems biology tools continues to identify genes involved in the biosynthesis of glucosinolates. recent progress includes genes in all three phases of the pathway, i.e. side-chain elongation of precursor amino acids, formation of the core glucosinolate structure and side-chain decoration. major breakthrough ... | 2010 | 20303821 |
| northern blotting techniques for small rnas. | in eukaryotes, rna silencing encompasses a range of biochemical processes mediated by approximately 20-25 nt small rnas (smrnas). this chapter describes northern blot hybridization techniques optimized for detection of such smrnas, whether extracted from plant or animal tissues. the basic protocol is described, and control blots illustrate the detection specificity and sensitivity of this method using dna oligonucleotide probes. known endogenous smrnas are analyzed in samples prepared from sever ... | 2010 | 20204871 |
| hsp70 and its cochaperone cpip promote potyvirus infection in nicotiana benthamiana by regulating viral coat protein functions. | this study demonstrates that heat shock protein 70 (hsp70) together with its cochaperone cpip regulates the function of a potyviral coat protein (cp), which in turn can interfere with viral gene expression. hsp70 was copurified as a component of a membrane-associated viral ribonucleoprotein complex from potato virus a-infected plants. downregulation of hsp70 caused a cp-mediated defect associated with replication. when pva cp was expressed in trans, it interfered with viral gene expression and r ... | 2010 | 20154150 |
| hc-pro, a potyvirus rna silencing suppressor, cancels cycling of cucumber mosaic virus in nicotiana benthamiana plants. | the mixed infection of cucumber mosaic virus (cmv) and a potyvirus has been known to increase cmv titer in nicotiana benthamiana plants, resulting in synergistic viral symptoms. we found that among three potyviruses--potato virus y (pvy), turnip mosaic virus (tumv), and clover yellow vein virus (c1yvv)--synergistic effects on cmv (or a recombinant cmv vector) titers were most efficiently induced by a co-infection with pvy in n. benthamiana plants. in addition, the helper component-proteinase (hc ... | 2010 | 20162445 |
| nucleolin/nsr1p binds to the 3' noncoding region of the tombusvirus rna and inhibits replication. | previous genome-wide screens identified >100 host genes affecting tombusvirus replication using yeast model host. one of those factors was nsr1p (nucleolin), which is an abundant rna-binding shuttle protein involved in rrna maturation and ribosome assembly. we find that overexpression of nsr1p in yeast or in nicotiana benthamiana inhibited the accumulation of tombusvirus rna by approximately 10-fold. regulated overexpression of nsr1p revealed that nsr1p should be present at the beginning of vira ... | 2010 | 19861225 |
| handygun: an improved custom-designed, non-vacuum gene gun suitable for virus inoculation. | particle bombardment with a non-vacuum gene gun is an efficient method for transfection of plant cells with cloned viruses and initiation of virus infection. the handygun developed in this study is an improved version of a non-vacuum gene gun. bombardment parameters were studied by inoculating an infectious, 35s promoter-driven cdna of potato virus a (pva; potyvirus) to the potato clone 'a6', nicotiana benthamiana and n. tabacum as plasmid dna coated on microprojectiles (gold particles). the lar ... | 2010 | 20188761 |
| multiple coat protein mutations abolish recognition of pepino mosaic potexvirus (pepmv) by the potato rx resistance gene in transgenic tomatoes. | despite the fact that pepino mosaic virus (pepmv) and potato virus x (pvx) share less than 40% identity in their coat proteins (cp), the known pvx elicitor of rx, transgenic tomato (cv. microtom) plants expressing a functional potato rx resistance gene showed resistance toward pepmv. however, in a low percentage of plants, pepmv accumulation was observed and back inoculation experiments demonstrated that these plants contained resistance-breaking pepmv variants. sequencing of the cp gene of thes ... | 2010 | 20192825 |
| immunogenic properties of chimeric potato virus x particles displaying the hepatitis c virus hypervariable region i peptide r9. | the immunogenic properties of chimeric potato virus x (pvx) particles engineered to display the synthetic r9 peptide have been evaluated. the r9 peptide is a consensus sequence derived from diverse variants of the hypervariable region 1 from the hepatitis c virus (hcv) envelope protein e2. two different constructs were designed, with the r9 peptide expressed either as an indirect fusion via the ribosomal skip 2a (pvx(r9-2a)cp) sequence or as a direct pvx coat protein fusion (pvx(r9)cp). systemic ... | 2010 | 20138085 |
| viral-induced systemic necrosis in plants involves both programmed cell death and the inhibition of viral multiplication, which are regulated by independent pathways. | resistant plants respond rapidly to invading avirulent plant viruses by triggering a hypersensitive response (hr). an hr is accompanied by a restraint of virus multiplication and programmed cell death (pcd), both of which have been observed in systemic necrosis triggered by a successful viral infection. here, we analyzed signaling pathways underlying the hr in resistance genotype plants and those leading to systemic necrosis. we show that systemic necrosis in nicotiana benthamiana, induced by pl ... | 2010 | 20121450 |
| identification of the virulence factors and suppressors of posttranscriptional gene silencing encoded by ageratum yellow vein virus, a monopartite begomovirus. | ageratum yellow vein disease (ayvd) is caused by the association of a tomato leaf curl java betasatellite [indonesia:indonesia 1:2003] (tolcjb-[id:id1:03]) with a begomovirus component. our previous results demonstrated that tolcjb-[id:id:03] is essential for induction of leaf curl symptoms in plants and transgene expression of its betac1 gene in nicotiana benthamiana plants induces virus-like symptoms. here we show that ageratum yellow vein virus-indonesia [indonesia: tomato] (ayvv-id[id:tom]) ... | 2010 | 20079777 |
| analysis of an autoproteolytic activity of rice yellow mottle virus silencing suppressor p1. | ectopically expressed rice yellow mottle virus p1 fusion proteins were found to be cleaved in planta and in escherichia coli. cleavage takes place in the absence of bacterial protease activity, indicating that the p1 fusion is autocatalytically processed independently of host factors. n-terminal sequencing of the c-terminal cleavage product of transiently expressed p1/gfp (green fluorescence protein) in nicotiana benthamiana showed that the cleavage site is located between the first two amino ac ... | 2010 | 20030588 |
| high-level rapid production of full-size monoclonal antibodies in plants by a single-vector dna replicon system. | plant viral vectors have great potential in rapid production of important pharmaceutical proteins. however, high-yield production of hetero-oligomeric proteins that require the expression and assembly of two or more protein subunits often suffers problems due to the "competing" nature of viral vectors derived from the same virus. previously we reported that a bean yellow dwarf virus (beydv)-derived, three-component dna replicon system allows rapid production of single recombinant proteins in pla ... | 2010 | 20047189 |
| covalent dimerization of camelidae anti-human tnf-alpha single domain antibodies by the constant kappa light chain domain improves neutralizing activity. | the tumor necrosis factor-alpha (tnfalpha) plays an important role in a number of chronic inflammatory disorders. monoclonal camelidae variable heavy chain domain-only antibodies (v(h)h) have been developed to antagonize the action of human tnfalpha (anti-tnf-v(h)h). here we describe a strategy to obtain functional dimeric anti-tnf-v(h)h molecules, based on the c-terminal fusion of a kappa light chain domain to the anti-tnf-v(h)h. the resulting fusion protein was transiently expressed by use of ... | 2010 | 20047190 |
| hydrophobin fusions for high-level transient protein expression and purification in nicotiana benthamiana. | insufficient accumulation levels of recombinant proteins in plants and the lack of efficient purification methods for recovering these valuable proteins have hindered the development of plant biotechnology applications. hydrophobins are small and surface-active proteins derived from filamentous fungi that can be easily purified by a surfactant-based aqueous two-phase system. in this study, the hydrophobin hfbi sequence from trichoderma reesei was fused to green fluorescent protein (gfp) and tran ... | 2010 | 20018596 |
| characterization of tomato yellow vein streak virus, a begomovirus from brazil. | tomato yellow vein streak virus (toyvsv) is a tentative begomovirus (family geminiviridae) species that seriously affects tomato and potato production in brazil. here, we have determined the genomic and biological characteristics of a toyvsv isolate (ba3) from a potato plant sampled in rio grande do sul state, brazil. the dna-a nucleotide sequence of ba3 and another previously reported toyvsv isolate share 89.7% sequence identity. these toyvsv isolates should be classified as a new species in th ... | 2010 | 19937269 |
| significance of eukaryotic translation elongation factor 1a in tobacco mosaic virus infection. | eukaryotic translation elongation factor 1a (eef1a) has been shown to interact with both the viral rna-dependent rna polymerase and the 3'-terminal genomic rna of tobacco mosaic virus (tmv). in this study, we demonstrated that the down-regulation of eef1a mrna levels by virus-induced gene silencing using potato virus x vector dramatically reduced the accumulation of tmv rna and the spread of tmv infection. the translation activity of the eef1a-silenced nicotiana benthamiana leaves was not severe ... | 2010 | 20012112 |
| an efficient system to detect protein ubiquitination by agroinfiltration in nicotiana benthamiana. | the ubiquitination proteasome pathway has been demonstrated to regulate all plant developmental and signaling processes. e3 ligase/substrate-specific interactions and ubiquitination play important roles in this pathway. however, due to technical limitations only a few instances of e3 ligase-substrate binding and protein ubiquitination in plants have been directly evidenced. an efficient in vivo and in vitro ubiquitination assay was developed for analysis of protein ubiquitination reactions by ag ... | 2010 | 20015064 |
| rna-dependent rna polymerase 6 delays accumulation and precludes meristem invasion of a viroid that replicates in the nucleus. | the detection of viroid-derived small rnas (vd-srnas) similar to the small interfering rnas (sirnas, 21 to 24 nucleotides [nt]) in plants infected by nuclear-replicating members of the family pospiviroidae (type species, potato spindle tuber viroid [pstvd]) indicates that they are inducers and targets of the rna-silencing machinery of their hosts. rna-dependent rna polymerase 6 (rdr6) catalyzes an amplification circuit producing the double-stranded precursors of secondary sirnas. recently, the r ... | 2010 | 20015979 |
| high level protein expression in plants through the use of a novel autonomously replicating geminivirus shuttle vector. | we constructed a novel autonomously replicating gene expression shuttle vector, with the aim of developing a system for transiently expressing proteins at levels useful for commercial production of vaccines and other proteins in plants. the vector, pric, is based on the mild strain of the geminivirus bean yellow dwarf virus (beydv-m) and is replicationally released into plant cells from a recombinant agrobacterium tumefaciens ti plasmid. pric differs from most other geminivirus-based vectors in ... | 2010 | 19929900 |
| the nuclear interactor pyl8/rcar3 of fagus sylvatica fspp2c1 is a positive regulator of abscisic acid signaling in seeds and stress. | the functional protein phosphatase type 2c from beechnut (fagus sylvatica; fspp2c1) was a negative regulator of abscisic acid (aba) signaling in seeds. in this report, to get deeper insight on fspp2c1 function, we aim to identify pp2c-interacting partners. two closely related members (pyl8/rcar3 and pyl7/rcar2) of the arabidopsis (arabidopsis thaliana) betv i family were shown to bind fspp2c1 in a yeast two-hybrid screening and in an aba-independent manner. by transient expression of fspp2c1 and ... | 2010 | 19889877 |
| the rice yellow mottle virus p1 protein exhibits dual functions to suppress and activate gene silencing. | in plants rna silencing is a host defense mechanism against viral infection, in which double-strand rna is processed into 21-24-nt short interfering rna (sirna). silencing spreads from cell to cell and systemically through a sequence-specific signal to limit the propagation of the virus. to counteract this defense mechanism, viruses encode suppressors of silencing. the p1 protein encoded by the rice yellow mottle virus (rymv) displays suppression activity with variable efficiency, according to t ... | 2010 | 19891703 |
| tomato leaf curl java virus v2 protein is a determinant of virulence, hypersensitive response and suppression of posttranscriptional gene silencing. | we previously identified the tomato leaf curl java virus-a (tolcjv-a[id]) from southeast asia as a new member of the emerging group of monopartite begomoviruses that require a betasatellite component for symptom induction. in this study, the role of v2 in viral pathogenesis and posttranscriptional gene silencing (ptgs) was studied. our results showed v2 of tolcjv-a[id] elicits a reaction resembling the hypersensitive response (hr) associated with the induction of necrosis and a systemic burst of ... | 2010 | 19896687 |
| potatovirus x and tobacco mosaic virus-based vectors compatible with the gateway cloning system. | virus-based expression vectors are important tools for high-level production of foreign proteins and for gene function analysis through virus induced gene silencing. to exploit further their advantages as fast, high yield replicons, a set of vectors was produced by converting and adapting potato virus x (pvx) and tobacco mosaic virus (tmv)-based vectors to allow easy cloning of foreign sequences by the gateway cloning system. target genes were cloned efficiently by recombination and successfully ... | 2010 | 19903495 |
| satellite panicum mosaic virus coat protein enhances the performance of plant virus gene vectors. | the coat protein of satellite panicum mosaic virus (spcp) is known to effectively protect its cognate rna from deleterious events, and here, we tested its stabilizing potential for heterologous virus-based gene vectors in planta. in support of this, a potato virus x (pvx) vector carrying the spmv capsid protein (pvx-spcp) gene was stable for at least three serial systemic passages through nicotiana benthamiana. to test the effect of spcp in trans, pvx-spcp was co-inoculated onto n. benthamiana t ... | 2010 | 19903565 |