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a carrot g-box binding factor-type basic region/leucine zipper factor dcbz1 is involved in abscisic acid signal transduction in somatic embryogenesis.carrot (daucus carota) somatic embryogenesis has been extensively used as an experimental system for studying embryogenesis. in maturing zygotic embryos, abscisic acid (aba) is involved in acquisition of desiccation tolerance and dormancy. on the other hand, somatic embryos contain low levels of endogenous aba and show desiccation intolerance and lack dormancy, but tolerance and dormancy can be induced by exogenous application of aba. in aba-treated carrot embryos, some aba-inducible genes are e ...201618407508
nutrient status and phytotoxicity analysis of goat manure discharged from farms in south korea.the present study evaluated the phytotoxicity effect of goat manure (gm) collected from six different regions in south korea, namely, chupungnyeong (t1), hoengseong (t2), goesan (t3), sancheong (t4), jangsu (t5) and namwon (t6). phytotoxicity was assessed by means of the analysis of germination index (gi), relative seed germination (rsg), relative root elongation (rre) and vigor index (vi) using five commercial crop varieties, namely, sesame (sesamum indicum l.), chrysanthemum (chrysanthemum ind ...201727654710
exogenous gibberellin enhances secondary xylem development and lignification in carrot taproot.gibberellins (gas) are important growth regulators involved in plant development processes. however, limited information is known about the relationship between ga and xylogenesis in carrots. in this study, carrot roots were treated with ga3. the effects of applied ga3 on root growth, xylem development, and lignin accumulation were then investigated. results indicated that ga treatment dose-dependently inhibited carrot root growth. the cell wall significantly thickened in the xylem parenchyma. a ...201727335006
bioassessment of heavy metals in the surface soil layer of an opencast mine aimed for its rehabilitation.the contemporary reclamation method in an opencast coal mine closure comprises the use of the preserved surface soil layer (ssl) before mining, and can be directly returned to the areas being rehabilitated. the present study emphasizes a risk in the use of such a ssl in mine rehabilitation due to the possible excessive amount of heavy metals which usually derives from a metal-rich sediment or fluvial character of overburden material. this indication was approved by the bioassessment of cadmium ( ...201727823905
characterising genes associated with flowering time in carrot (daucus carota l.) using transcriptome analysis.carrot is generally regarded as a biennial plant with an obligatory vernalization requirement. early spring cultivation makes plants vulnerable to premature bolting, which results in a loss of commercial value. however, our knowledge of flowering time genes and flowering mechanisms in carrot remain limited. bolting behavior of d. carota ssp. carota 'songzi', a wild species sensitive to flower induction by vernalization and photoperiod, and orange cultivar 'amsterdam forcing', and their offspring ...201727775866
effects of chrysosporum (aphanizomenon) ovalisporum extracts containing cylindrospermopsin on growth, photosynthetic capacity, and mineral content of carrots (daucus carota).natural toxins produced by freshwater cyanobacteria, such as cylindrospermopsin, have been regarded as an emergent environmental threat. despite the risks for food safety, the impact of these water contaminants in agriculture is not yet fully understood. carrots (daucus carota) are root vegetables, extensively consumed worldwide with great importance for human nourishment and economy. it is, therefore, important to evaluate the possible effects of using water contaminated with cyanotoxins on car ...201727770233
assessing the impact of geogenic chromium uptake by carrots (daucus carota) grown in asopos river basin.a methodology was developed to assess the impact of geogenic origin hexavalent chromium uptake by carrots, and the risk of human consumption of carrots grown in asopos river basin in greece. a field scale experiment was conducted with carrots cultivated in treatment plots, with and without compost amendment, in order to assess the impact of carbon in the mobility and uptake of chromium by plants. the results suggested that there is a trend for chromium mobilization and uptake in the surface and ...201727764685
analysis of the use of microcystin-contaminated water in the growth and nutritional quality of the root-vegetable, daucus carota.toxic cyanobacterial blooms are often observed in freshwaters and may reflect the increased eutrophication of these environments and alterations in climate. cyanotoxins, such as microcystins (mcs), are an effective threat to many life forms, ranging from plants to humans. despite the research conducted to date on cyanotoxins, the risks associated to the use of contaminated water in agriculture require further elucidation. to tackle this aim, a research was conducted with the root-vegetable daucu ...201727752954
glutathione sulfotransferase inhibition activity of a self-fermented beverage, kanji.kanji, a liquid preparation of roots of daucus carota l. ssp. sativus (hoffm.) arcang. var. vavilovii mazk. (apiaceae), may inhibit glutathione sulfotransferase (gst) activity due to ferulic acid content.201727951746
carotenoid gene expression explains the difference of carotenoid accumulation in carrot root tissues.main conclusion variations in gene expression can partially explain the difference of carotenoid accumulation in secondary phloem and xylem of fleshy carrot roots. the carrot root is well divided into two different tissues separated by vascular cambium: the secondary phloem and xylem. the equilibrium between these two tissues represents an important issue for carrot quality, but the knowledge about the respective carotenoid accumulation is sparse. the aim of this work was (i) to investigate if v ...201727999990
wild carrot pentane-based fractions suppress proliferation of human hacat keratinocytes and protect against chemically-induced skin cancer.previous studies in our laboratory showed that the lebanese daucus carota ssp. carota (wild carrot) oil extract possesses in vitro and in vivo anticancer activities. the present study aims to examine the cytotoxic effect of daucus carota oil fractions on human epidermal keratinocytes and evaluate the chemopreventive activity of the pentane diethyl ether fraction on dmba/tpa induced skin carcinogenesis in mice.201728073348
characterization of a genomic region under selection in cultivated carrot (daucus carota subsp. sativus) reveals a candidate domestication gene.carrot is one of the most important vegetables worldwide, owing to its capability to develop fleshy, highly nutritious storage roots. it was domesticated ca. 1,100 years ago in central asia. no systematic knowledge about the molecular mechanisms involved in the domestication syndrome in carrot are available, however, the ability to form a storage root is undoubtedly the essential transition from the wild daucus carota to the cultivated carrot. here, we expand on the results of a previous study w ...201728149306
foliar-applied ethephon enhances the content of anthocyanin of black carrot roots (daucus carota ssp. sativus var. atrorubens alef.).black carrots (daucus carota ssp. sativus var. atrorubens alef.) constitute a valuable source of anthocyanins, which are used as natural red, blue and purple food colourants. anthocyanins and phenolic compounds are specialised metabolites, accumulation of which often requires elicitors, which act as molecular signals in plant stress responses. in the present study, ethephon, an ethylene-generating compound was explored as enhancer of anthocyanin and phenolic contents during growth of 'deep purpl ...201728376712
different lengths, copies and expression levels of the mitochondrial atp6 gene in male sterile and fertile lines of carrot (daucus carota l.).the male-sterile carrot is an effective material for carrot breeding. the atp6 gene is involved in carrot fertility. however, the differences in lengths, copies, and expression profiles of the atp6 gene in fertile and male-sterile lines of carrot are unclear. in this study, one copy atp6 gene was found in the mtdnas of 'kuroda' (fertility, 954 bp) and 'wuye-by' (male sterility, 819 bp) carrot lines, while two copies atp6 genes (wuye-l and wuye-d, 954 bp and 819 bp, respectively) were found in th ...201728335670
carrot solution culture bioproduction of uniformly labeled (13)c-lutein and in vivo dosing in non-human primates.lutein is a xanthophyll abundant in nature and most commonly present in the human diet through consumption of leafy green vegetables. with zeaxanthin and meso-zeaxanthin, lutein is a component of the macular pigment of the retina, where it protects against photooxidation and age-related macular degeneration. recent studies have suggested that lutein may positively impact cognition throughout the lifespan, but outside of the retina, the deposition, metabolism, and function(s) of lutein are poorly ...201727798119
carrot (daucus carota l.): nephroprotective against gentamicin-induced nephrotoxicity in rats.daucus carota l.(dc) commonly known as carrot, folkorically used as ethnomedicine to treat nephrosis and other urinary disorders. hence, the present study was aimed to investigate the nephroprotective effects of ethanolic root extract of dc against gentamicin-induced nephrotoxicity in albino wistar rats.201727127313
anti-inflammatory potential of black carrot (daucus carota l.) polyphenols in a co-culture model of intestinal caco-2 and endothelial ea.hy926 cells.the present study was developed to determine the ability of polyphenol-rich black carrot and its by-products, i.e., peel and pomace, to modulate the inflammatory response in tumor necrosis factor α (tnf-α) treated endothelial cells after gastrointestinal digestion and in a co-culture of intestinal caco-2 and endothelial ea.hy926 cell model.201727561918
a novel β-carotene-associated carrot (daucus carota l.) pectic polysaccharide.carrot (daucus carota l.) is a nutritional source with enriched β-carotene (0.01%) and pectins (0.8%). although studies have highlighted the association between β-carotene and carrot pectic polysaccharide (crpp), the precise binding of β-carotene to crpp and its biological implications were not yet clearly understood. the aim of the present study is to report for the first time a natural carbohydrate polymer-crpp-with associated β-carotene.201727068881
inhibitory activity and chemical characterization of daucus carota subsp. maximus essential oils.the essential oils (eos) of green seeds from daucus carota subsp. maximus growing wild in pantelleria island (sicily, italy) were characterised. eos were extracted by steam distillation, examined for their inhibitory properties against food-borne gram positive and gram negative bacteria and analysed for the chemical composition by gas chromatography (gc) and mass spectrometry (ms). undiluted eos showed a large inhibition spectrum against gram positive strains and also vs acinetobacter spp. and s ...201728171692
identification of candidates for interacting partners of the tail domain of dcnmcp1, a major component of the daucus carota nuclear lamina-like structure.nmcp/crwn (nuclear matrix constituent protein/crowded nuclei) is a major component of a protein fibrous meshwork (lamina-like structure) on the plant inner nuclear membrane. nmcp/crwn contributes to regulating nuclear shape and nuclear functions. an nmcp/crwn protein in daucus carota (dcnmcp1) is localized to the nuclear periphery in interphase cells, and surrounds chromosomes in cells in metaphase and anaphase. the n-terminal region and the c-terminal region of dcnmcp1 are both necessary for lo ...201728146370
stereoselective reduction of prochiral ketones by plant and microbial biocatalysts.chiral alcohols are the key chiral building blocks to many enantiomerically pure pharmaceuticals. the biocatalytic approach in asymmetric reduction of corresponding prochiral ketones to the preparation of these optically pure substances is one of the most promising routes. the stereoselective reduction of different kinds of prochiral ketones catalyzed by various plants and microorganisms was studied in this work. benzyl acetoacetate, methyl 3-oxopentanoate, ethyl 3-oxopentanoate, and ethyl butyr ...201727168684
antibacterial, antifungal, and antiviral effects of three essential oil blends.new agents that are effective against common pathogens are needed particularly for those resistant to conventional antimicrobial agents. essential oils (eos) are known for their antimicrobial activity. using the broth microdilution method, we showed that (1) two unique blends of cinnamomum zeylanicum, daucus carota, eucalyptus globulus and rosmarinus officinalis eos (ab1 and ab2; cinnamon eos from two different suppliers) were active against the fourteen gram-positive and -negative bacteria stra ...201728296357
laser capture microdissection for amplification of alternative oxidase (aox) genes in target tissues in daucus carota l.laser microdissection provides a useful method for isolating specific cell types from complex biological samples for downstream applications. in contrast to the texture of mammalian cells, most plant tissues exhibit a cell organization with hard, cellulose-containing cell walls, large vacuoles, and air spaces, thus complicating tissue preparation and extraction of macromolecules such as dna. in this study, we report a method that allows tissue-specific gene amplification. an improved perception ...201728871549
studying individual plant aox gene functionality in early growth regulation: a new approach.aox1 and aox2 genes are thought to play different physiological roles. whereas aox1 is typically expected to associate to stress and growth responses, aox2 was more often found to be linked to development and housekeeping functions. however, this view is questioned by several adverse observations. for example, co-regulated expression for dcaox1 and dcaox2a genes was recently reported during growth induction in carrot (daucus carota l.). early expression peaks for both genes during the lag phase ...201728871548
evaluating the antibacterial properties of polyacetylene and glucosinolate compounds with further identification of their presence within various carrot (daucus carota) and broccoli (brassica oleracea) cultivars using high-performance liquid chromatography with a diode array detector and ultra performance liquid chromatography-tandem mass spectrometry analyses.ongoing consumer concerns over using synthetic additives in foods has strongly influenced efforts worldwide to source suitable natural alternatives. in this study, the antibacterial efficacy of polyacetylene and glucosinolate compounds was evaluated against both gram positive and gram negative bacterial strains. falcarinol [minimum inhibitory concentration (mic) = 18.8-37.6 μg/ml] demonstrated the best overall antibacterial activity, while sinigrin (mic = 46.9-62.5 μg/ml) was the most active glu ...201728805380
third-harmonic generation stokes-mueller polarimetric microscopy.an experimental implementation of the nonlinear stokes-mueller polarimetric (nsmp) microscopy in third-harmonic generation modality is presented. the technique is able to extract all eight 2d-accessible χ(3) components for any sample from 64 polarization measurements, and can be applied to noninvasive ultrastructural characterization. the polarization signature of an isotropic glass coverslip is presented, and carotenoid crystallites in the root of orange carrot (daucus carota) are investigated, ...201728788853
antitumor activity of β-2-himachalen-6-ol in colon cancer is mediated through its inhibition of the pi3k and mapk pathways.previous studies in our laboratory showed that daucus carota oil extract (dcoe) possesses in vitro and in vivo anticancer activities. chemical analysis of dcoe led to the isolation of β-2-himachalen-6-ol (hc) which exhibited potent anticancer activity against colon, breast, brain and skin cancer cells. the present study investigates the anticancer activity of hc against sw1116 colon cancer cell lines, and evaluates its effect in a 1,2-dimethylhydrazine (dmh) colon carcinogenesis black6 mice mode ...201728782499
nicotinamide; antioxidative and dna hypomethylation effects in plant cells.the effects of nicotinamide (nic) and its natural plant metabolites nicotinic acid (nia) and trigonelline (trig) were studied with respect to defense in plant cell cultures. nic and nia could protect against oxidative stress damage caused by 2,2'-azobis(2-amidinopropane) dihydrochloride (aaph), which generates free radicals. damage was analyzed as dna strand breaks in cell cultures of pisum sativum (garden pea), daucus carota (carrot), populus tremula l. × p. tremuloides (hybrid aspen) and catha ...201728780454
miniature inverted repeat transposable element insertions provide a source of intron length polymorphism markers in the carrot (daucus carota l.).the prevalence of non-autonomous class ii transposable elements (tes) in plant genomes may serve as a tool for relatively rapid and low-cost development of gene-associated molecular markers. miniature inverted-repeat transposable element (mite) copies inserted within introns can be exploited as potential intron length polymorphism (ilp) markers. ilps can be detected by pcr with primers anchored in exon sequences flanking the target introns. here, we designed primers for 209 dcsto (daucus carota ...201728536590
asymmetric bio- and chemoreduction of 2-benzylidenecyclopentanone derivatives.highly efficient asymmetric reduction of 2-benzylidenecyclopentanone derivatives to give the respective exocyclic allylic alcohols in ee's up to 96% was performed with chiral oxazaborolidine-based catalysts. complete enantioselectivity furnishing (s)-configured alcohol product could be achieved by bioreduction of 2-(4-chlorobenzylidene)cyclopentanone with daucus carota root. the synthesized compounds may be used as enantiomerically enriched standards for the monitoring of the enzyme-catalyzed re ...201729318315
effect of cetyltrimethyl ammonium bromide on uptake of polycyclic aromatic hydrocarbons by carrots.this is the first study investigating the effect of cationic surfactants on the mobility of polycyclic aromatic hydrocarbons (pahs) in aged contaminated soils and on pah bioaccumulation in tuberous vegetables. in an aerobic soil incubation experiment, 150 mg/kg cetyltrimethyl ammonium bromide (ctmab) decreased the bioavailability of pahs primarily via immobilization (by 13%). in a carrot pot experiment, the effectiveness of ctmab to reduce pah uptake by carrots decreased with time. accordingly, ...201729277355
queen anne's lace, gooseneck loosestrife, untitled (poppy maidenhair fern), blushing deathcap, untitled (trillium), and untitled (trillium, pear blossom, salamander) by victor schrager.recognizing the contribution art has had in the mayo clinic environment since the original mayo clinic building was finished in 1914, mayo clinic proceedings features some of the numerous works of art displayed throughout the buildings and grounds on mayo clinic campuses as interpreted by the author.201729202948
dissecting the genetic architecture of shoot growth in carrot (daucus carota l.) using a diallel mating design.carrot production suffers yield losses under weed pressure, and thus improved competitive ability is a breeding priority. however, continued improvement and in-depth genetic studies for these traits relies on knowledge of the underlying genetic architecture. this study estimated heritable and non-heritable components of carrot shoot growth from a diallel mating design using a bayesian mixed model. results directly contribute to improvement efforts by ranking hybrids, identifying useful tester li ...201729187419
the terpene synthase gene family of carrot (daucus carota l.): identification of qtls and candidate genes associated with terpenoid volatile compounds.terpenes are an important group of secondary metabolites in carrots influencing taste and flavor, and some of them might also play a role as bioactive substances with an impact on human physiology and health. understanding the genetic and molecular basis of terpene synthases (tps) involved in the biosynthesis of volatile terpenoids will provide insights for improving breeding strategies aimed at quality traits and for developing specific carrot chemotypes possibly useful for pharmaceutical appli ...201729170675
emergence of leuconostoc mesenteroides as a causative agent of oozing in carrots stored under non-ventilated conditions.long-term storage and transport of post-harvest carrots (daucus carota l.) require a low-temperature, high-relative-humidity environment, usually with low ventilation. following long-term storage, a slimy exudate (oozing) often appears on the carrots, leading to severe spoilage. we characterized the environmental conditions leading to these symptoms and identified the causative agent. simulation of non-ventilated storage conditions revealed accumulation of co2 (to 80%) and ethanol (to 1000 ppm); ...201728834204
effects of cover crops on pratylenchus penetrans and the nematode community in carrot production.cover cropping is a common practice in u.s. midwest carrot production for soil conservation, and may affect soil ecology and plant-parasitic nematodes-to which carrots are very susceptible. this study assessed the impact of cover crops-oats (avena sativa), radish (raphanus sativus) cv. defender, rape (brassica napus) cv. dwarf essex, and a mixture of oats and radish-on plant-parasitic nematodes and soil ecology based on the nematode community in michigan carrot production systems. research was c ...201728512383
an enhancement of antimicrobial efficacy of biogenic and ceftriaxone-conjugated silver nanoparticles: green approach.of the various methods explored for the synthesis of nanoparticles, biogenesis of silver nanoparticles (agnps) received great attention due to their versatile properties. in this report, daucus carota extract was used for the synthesis of agnps and ceftriaxone was conjugated with agnps to enhance their antimicrobial efficacy. the conjugated and unconjugated agnps were characterized by adopting uv-vis spectroscopy, ftir, afm, dls, and tem, which revealed the spr peak at 420 nm and spherical shape ...201728600792
heterologous expression of the carrot hsp17.7 gene increased growth, cell viability, and protein solubility in transformed yeast (saccharomyces cerevisiae) under heat, cold, acid, and osmotic stress conditions.in industrial fermentation of yeast (saccharomyces cerevisiae), culture conditions are often modified from the optimal growth conditions of the cells to maintain large-scale cultures and/or to increase recombinant protein production. however, altered growth conditions can be stressful to yeast cells resulting in reduced cell growth and viability. in this study, a small heat shock protein gene from carrot (daucus carota l.), hsp17.7, was inserted into the yeast genome via homologous recombination ...201728573339
assessment of the potential health risks associated with the aluminium, arsenic, cadmium and lead content in selected fruits and vegetables grown in jamaica.thirteen jamaican-grown food crops - ackee (blighia sapida), banana (musa acuminate), cabbage (brassica oleracea), carrot (daucus carota), cassava (manihot esculenta), coco (xanthosoma sagittifolium), dasheen (colocasia esculenta), irish potato (solanum tuberosum), pumpkin (cucurbita pepo), sweet pepper (capsicum annuum), sweet potato (ipomoea batatas), tomato (solanum lycopersicum) and turnip (brassica rapa) - were analysed for aluminium, arsenic, cadmium and lead by atomic absorption spectroph ...201728959639
distinct transcription profile of genes involved in carotenoid biosynthesis among six different color carrot (daucus carota l.) cultivars.carotenoid, a group of lipophilic molecules, is widely distributed in nature, and is important for plant photosynthesis and photoprotection. in carrot taproot, different types of dominant carotenoid accumulation lead to yellow, orange, and red colors. in this study, six different carrot cultivars were used to simultaneously analyze carotenoid contents by high performance liquid chromatography. the expression levels of genes involved in carotenoid biosynthesis of carrot were also detected by real ...201728910981
β-2-himachalen-6-ol protects against skin cancer development in vitro and in vivo.previous studies in our laboratory showed that daucus carota oil extract (dcoe) possesses remarkable in-vitro anticancer activity and antitumour promoting effect against dmba/tpa skin carcinogenesis in mice. chemical analysis of dcoe led to the isolation of the β-2-himachalen-6-ol (hc), major sesquiterpene with a potent anticancer activity against various colon, breast, brain and skin cancer cells. this study investigated the anticancer activity of hc against invasive epidermal squamous cell car ...201728872682
toxic metal tolerance in native plant species grown in a vanadium mining area.vanadium (v) has been extensively mined in china and caused soil pollution in mining area. it has toxic effects on plants, animals and humans, posing potential health risks to communities that farm and graze cattle adjacent to the mining area. to evaluate in situ phytoremediation potentials of native plants, v, chromium, copper and zinc concentrations in roots and shoots were measured and the bioaccumulation (baf) and translocation (tf) efficiencies were calculated. the results showed that setar ...201728963601
simultaneous determination of fluoroquinolones and sulfonamides originating from sewage sludge compost.a simultaneous method for quantitative determination of traces of fluoroquinolones (fqs) and sulfonamides (sas) in edible plants fertilized with sewage sludge was developed. the compounds were extracted from the plants by rapid and simple liquid extraction followed by extracts clean-up using solid phase extraction. the eluent additive 1,1,1,3,3,3-hexafluoro-2-propanol was used for liquid chromatographic detection to achieve separation of structurally similar antimicrobials like ciprofloxacin and ...201728695191
adaptogenic potential of oxitard in experimental chronic stress and chronic unpredictable stress induced dysfunctional homeostasis in rodents.oxitard, a polyherbal formulation comprising the extracts of withania somnifera, mangifera indica, glycyrrhiza glabra, daucus carota, vitis vinifera, powders of syzygium aromaticum, yashada bhasma and emblica officinalis; and oils of triticum sativum.201728693955
fine mapping, transcriptome analysis, and marker development for y2 , the gene that conditions β-carotene accumulation in carrot (daucus carota l.).domesticated carrots, daucus carota subsp. sativus, are the richest source of β-carotene in the us diet, which, when consumed, is converted into vitamin a, an essential component of eye health and immunity. the y2 locus plays a significant role in beta-carotene accumulation in carrot roots, but a candidate gene has not been identified. to advance our understanding of this locus, the genetic basis of β-carotene accumulation was explored by utilizing an advanced mapping population, transcriptome a ...201728663343
morphological and molecular characteristics of pratylenchus haiduongensis sp. n., a new species of root-lesion nematodes associated with carrot in vietnam.pratylenchus haiduongensis sp. n. is described as associated with carrot (daucus carota subsp. sativus (hoffm.) schübl. & g. martens) in hai duong province, vietnam. p. haiduongensis sp. n. is characterized by the lip region with three annuli and slightly separated from the body. stylet knobs are rounded (never indented anteriorly). the lateral field includes four incisures, bearing areolation at the pharynx region and tail region and occasionally appears in the vulval region. sometimes the appe ...201729062150
respiration traits as novel markers for plant robustness under the threat of climate change: a protocol for validation.respiration traits allow calculating temperature-dependent carbon use efficiency and prediction of growth rates. this protocol aims (1) to enable validation of respiration traits as non-dna biomarkers for breeding on robust plants in support of sustainable and healthy plant production; (2) to provide an efficient, novel way to identify and predict functionality of dna-based markers (genes, polymorphisms, edited genes, transgenes, genomes, and hologenomes), and (3) to directly help farmers select ...201728871543
tween® 20-enhanced bioreduction of acetophenones 
promoted by daucus carota root.the effect of surfactants on the bioreduction of acetophenones mediated by carrot (daucus carota) root was investigated. among the tested surfactants, tween® 20 slightly improved the conversion. the amount of surfactant was optimised, and the presented methodology was applied to other substituted acetophenones, which led to the preparation of their respective optically enriched 1-phenylethanols with high conversion levels and high enantioselectivities.201728867953
transcriptional regulation of brassinosteroid accumulation during carrot development and the potential role of brassinosteroids in petiole elongation.it is widely known that brassinosteroids (brs) are involved in various physiological processes during plant growth and development. roles of brs have been reported in many plants. however, relevant report is yet not found in carrot. carrot is a nutrient-rich vegetable from the apiaceae family. here, we measured the bioactive contents of brs at five successive stages and analyzed the expression profiles of genes involved in br biosynthesis, signaling pathway and catabolism. we found that most bio ...201728848570
polyphenolic profile and biological activities of black carrot crude extract (daucus carota l. ssp. sativus var. atrorubens alef.).black carrot (daucus carota l. ssp. sativus var. atrorubens alef.) is a valuable source of carbohydrates, minerals and vitamins and contains also high amounts of anthocyanins giving the characteristic deep-purple color. these latter compounds are known as natural dyes used in the food and pharmaceutical industry that have recently attracted much attention for their healthful properties. the aim of this work was to investigate for the first time the polyphenolic profile and biological properties ...201829050970
ozone treatment for pesticide removal from carrots: optimization by response surface methodology.the present study aimed to optimize ozone (o3) treatments, as gas and dissolved in water, to remove difenoconazole and linuron in carrots. we employed a central composite design to study three variables governing the efficacy of treatments: o3 concentration, temperature and treatment time. the temperature did not influence the efficacy of treatments. the removal percentage of pesticides increases with increases in ozone concentration and the time of treatment. o3 application promoted the removal ...201829146362
efficient crispr/cas9-based genome editing in carrot cells.the first report presenting successful and efficient carrot genome editing using crispr/cas9 system. clustered regularly interspaced short palindromic repeats (crispr)/crispr-associated (cas9) is a powerful genome editing tool that has been widely adopted in model organisms recently, but has not been used in carrot-a model species for in vitro culture studies and an important health-promoting crop grown worldwide. in this study, for the first time, we report application of the crispr/cas9 system ...201829332168
may humic acids or mineral fertilisation mitigate arsenic mobility and availability to carrot plants (daucus carota l.) in a volcanic soil polluted by as from irrigation water?carrot (daucus carota l.) is a widely consumed root vegetable, whose growth and safety might be threatened by growing-medium arsenic (as) contamination. by this work, we evaluated the effects of humic acids from leonardite and npk mineral fertilisation on as mobility and availability to carrot plants grown for 60 days in a volcanic soil irrigated with as-contaminated water - representing the most common scenario occurring in as-affected italian areas. as expected, the irrigation with as-contamin ...201829156331
salicylaldehyde synthase activity from venturia inaequalis elicitor-treated cell culture of apple.salicylic acid (sa) is known to trigger a number of plant defense responses upon pathogen attack. it is well known that apple (malus domestica) plants respond to pathogen invasion by synthesizing sa, but its biosynthesis is not well understood. in this study, we report salicylaldehyde synthase (sas) activity from venturia inaequalis elicitor (vie)-treated cell suspension cultures of apple (malus domestica 'florina'). sas catalyzes non-oxidative c2-side chain cleavage of 2-coumaric acid to form s ...201829247889
transcript profiling of sucrose synthase genes involved in sucrose metabolism among four carrot (daucus carota l.) cultivars reveals distinct patterns.carrot which contains lots of nutrients has a large demand around the world. the soluble sugar content in fleshy root of carrot directly influences its taste and quality. sucrose, as an important member of soluble sugar, is the main product of photosynthesis in higher plants and it plays pivotal roles in physiological processes including energy supply, signal transduction, transcriptional regulation, starch and cellulose synthesis, and stress tolerance. sucrose synthase is a key enzyme involved ...201829304728
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